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Carl Roth GmbH m nacl
M Nacl, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m nacl/product/Carl Roth GmbH
Average 92 stars, based on 4 article reviews
Price from $9.99 to $1999.99
m nacl - by Bioz Stars, 2021-01
92/100 stars

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Sonication:

Article Title: PqsL uses reduced flavin to produce 2-hydroxylaminobenzoylacetate, a preferred PqsBC substrate in alkyl quinolone biosynthesis in Pseudomonas aeruginosa
Article Snippet: .. For preparation of cell-free protein extracts, pellets were thawed, suspended in 20 m m Tris-Cl, pH 8, 150 m m NaCl, and 0.1% Nonidet P-40, and sonicated for four times for 2 min. Lysates were centrifuged (40 min, 25,000 × g , 4 °C) and filtered through a 0.45-μm polyvinylidene difluoride syringe filter (Carl Roth, Karlsruhe, Germany). .. PqsL was purified by means of either a hexahistidine or a StrepII tag fused to the N terminus.

Centrifugation:

Article Title: Role of Nucleotide Binding and GTPase Domain Dimerization in Dynamin-like Myxovirus Resistance Protein A for GTPase Activation and Antiviral Activity *
Article Snippet: .. Following centrifugation, bacterial pellets were resuspended in ice-cold 50 m m HEPES (pH 7.5), 800 m m NaCl, 30 m m imidazole, 5 m m MgCl2 , 1 μ m DNase I, 2.5 m m β-mercaptoethanol (β-ME), 500 μ m Pefabloc SC (Roth) and lysed in a microfluidizer (Microfluidics). ..

Article Title: The GTPase Activity of Murine Guanylate-binding Protein 2 (mGBP2) Controls the Intracellular Localization and Recruitment to the Parasitophorous Vacuole of Toxoplasma gondii *
Article Snippet: .. 4 h after induction, cells were harvested by centrifugation at 4000 × g , resuspended in the high salt buffer (50 m m Tris-HCl (pH 8.0), 5 m m MgCl2 , 300 m m NaCl, 10 m m imidazole, 1 m m β-mercaptoethanol) and 250 μ m Pefabloc (Roth), and disrupted in the Constant Cell Disruption System (Constant Cell) at a pressure of 2.7 kbars. .. After centrifugation for 1 h at 205,000 × g, the supernatant was applied to a Ni-NTA-Superflow column (Qiagen) with a bed volume of 25 ml connected to an ÄKTA prime System (GE Healthcare).

Lysis:

Article Title: Epstein-Barr Viral BNLF2a Protein Hijacks the Tail-anchored Protein Insertion Machinery to Block Antigen Processing by the Transport Complex TAP *
Article Snippet: .. Membranes were solubilized in 1 ml of lysis buffer L1 (20 m m Tris/HCl (pH 7.5), 150 m m NaCl, 5 m m MgCl2 , 2% (w/v) digitonin (Carl Roth, Karlsruhe, Germany)). .. Membranes isolated from Sf 9 insect cells (1 mg of total protein) or 2 × 106 transfected HeLa cells, respectively, were resuspended in 0.5 ml lysis buffer as described above.

Article Title: β-Secretase BACE1 Promotes Surface Expression and Function of Kv3.4 at Hippocampal Mossy Fiber Synapses
Article Snippet: .. Cells were resuspended in lysis buffer [50 m m Tris-HCl, pH 7.4; Sigma-Aldrich), 150 m m NaCl (Carl Roth), 2 m m EDTA (Carl Roth), 1% NP-40 (Sigma-Aldrich), 1% Triton X-100 (Sigma-Aldrich), and cOmplete MINI EDTA-free protease inhibitor mixture (Roche)]. .. Lysates were sonicated for 5 min using a water bath ultrasonic device (VWR), incubated on ice for 30 min, sonicated for another 5 min, and centrifuged at 6000 × g for 10 min.

Blocking Assay:

Article Title: Establishment of a Heterologous RNA Editing Event in Chloroplasts 1Establishment of a Heterologous RNA Editing Event in Chloroplasts 1 [OPEN]
Article Snippet: .. Blocking was performed in TBS-T buffer [20 m m Tris-HCl (pH 7.6), 137 m m NaCl, Tween 20 0.1% (w/v)] in the presence of 4% (w/v) skimmed milk powder and 0.5% (w/v) bovine serum albumin (Carl Roth GmbH) for 1 h at room temperature under continuous shaking. ..

Protease Inhibitor:

Article Title: A Membrane-bound Hemoglobin from Gills of the Green Shore Crab Carcinus maenas *
Article Snippet: .. Selected tissues from C. maenas were homogenized in 10 m m Tris/HCl, pH 7.4, 10 m m NaCl, 5 m m MgCl2 , 1 m m DTT, 0.1% Nonidet P40, 1 m m Pefabloc SC protease inhibitor (Roth) and Complete protease inhibitor mixture (Roche Applied Science). ..

Article Title: β-Secretase BACE1 Promotes Surface Expression and Function of Kv3.4 at Hippocampal Mossy Fiber Synapses
Article Snippet: .. Cells were resuspended in lysis buffer [50 m m Tris-HCl, pH 7.4; Sigma-Aldrich), 150 m m NaCl (Carl Roth), 2 m m EDTA (Carl Roth), 1% NP-40 (Sigma-Aldrich), 1% Triton X-100 (Sigma-Aldrich), and cOmplete MINI EDTA-free protease inhibitor mixture (Roche)]. .. Lysates were sonicated for 5 min using a water bath ultrasonic device (VWR), incubated on ice for 30 min, sonicated for another 5 min, and centrifuged at 6000 × g for 10 min.

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  • 90
    Carl Roth GmbH m nacl carl roth
    ORFV stability after freeze/thawing with and without sugar supplementation. Infective ORFV titers in SXC elution buffer (20 mM TRIS-HCL pH 7.4 with 0.4 M NaCl), supplemented with 0 %, 5 % and 10 % sucrose. The quantification was done by flow cytometry, directly after the purification and after each of up to three freeze/thaw cycles. Error bars indicate the standard deviation of technical triplicates.
    M Nacl Carl Roth, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m nacl carl roth/product/Carl Roth GmbH
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m nacl carl roth - by Bioz Stars, 2021-01
    90/100 stars
      Buy from Supplier

    92
    Carl Roth GmbH m nacl
    ORFV stability after freeze/thawing with and without sugar supplementation. Infective ORFV titers in SXC elution buffer (20 mM TRIS-HCL pH 7.4 with 0.4 M NaCl), supplemented with 0 %, 5 % and 10 % sucrose. The quantification was done by flow cytometry, directly after the purification and after each of up to three freeze/thaw cycles. Error bars indicate the standard deviation of technical triplicates.
    M Nacl, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m nacl/product/Carl Roth GmbH
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    m nacl - by Bioz Stars, 2021-01
    92/100 stars
      Buy from Supplier

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    ORFV stability after freeze/thawing with and without sugar supplementation. Infective ORFV titers in SXC elution buffer (20 mM TRIS-HCL pH 7.4 with 0.4 M NaCl), supplemented with 0 %, 5 % and 10 % sucrose. The quantification was done by flow cytometry, directly after the purification and after each of up to three freeze/thaw cycles. Error bars indicate the standard deviation of technical triplicates.

    Journal: Journal of Biotechnology

    Article Title: Selection of chromatographic methods for the purification of cell culture-derived Orf virus for its application as a vaccine or viral vector

    doi: 10.1016/j.jbiotec.2020.07.023

    Figure Lengend Snippet: ORFV stability after freeze/thawing with and without sugar supplementation. Infective ORFV titers in SXC elution buffer (20 mM TRIS-HCL pH 7.4 with 0.4 M NaCl), supplemented with 0 %, 5 % and 10 % sucrose. The quantification was done by flow cytometry, directly after the purification and after each of up to three freeze/thaw cycles. Error bars indicate the standard deviation of technical triplicates.

    Article Snippet: After sample application, the column was washed with equilibration buffer (at least 30 column volumes) and the virus was finally eluted using PBS, or 20 mM TRIS-HCl buffer pH 7.4 without PEG, but supplemented with 0.4 M NaCl (Carl Roth).

    Techniques: Flow Cytometry, Purification, Standard Deviation