m mulv reverse transcriptase  (New England Biolabs)


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    New England Biolabs m mulv reverse transcriptase
    M Mulv Reverse Transcriptase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m mulv reverse transcriptase/product/New England Biolabs
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    m mulv reverse transcriptase - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Evolutionary and epidemiological analyses based on spike genes of porcine epidemic diarrhea virus circulating in Thailand in 2008-2015.
    Article Snippet: Paragraph title: Cloning, plasmid purification and sequence determination ... Briefly, total RNA was extracted from the supernatant using the Nucleospin®RNA Virus kit (Macherey-Nagel Inc., PA, USA) in accordance with the manufacturer's instructions. cDNA was synthesized from the extracted RNA using M-MuLV Reverse Transcriptase (New England BioLabs Inc., MA, USA).

    Amplification:

    Article Title: Evolutionary and epidemiological analyses based on spike genes of porcine epidemic diarrhea virus circulating in Thailand in 2008-2015.
    Article Snippet: Briefly, total RNA was extracted from the supernatant using the Nucleospin®RNA Virus kit (Macherey-Nagel Inc., PA, USA) in accordance with the manufacturer's instructions. cDNA was synthesized from the extracted RNA using M-MuLV Reverse Transcriptase (New England BioLabs Inc., MA, USA). .. Cloning, plasmid purification and sequence determination PCR amplification was performed on the cDNA.

    Synthesized:

    Article Title: Evolutionary and epidemiological analyses based on spike genes of porcine epidemic diarrhea virus circulating in Thailand in 2008-2015.
    Article Snippet: .. Briefly, total RNA was extracted from the supernatant using the Nucleospin®RNA Virus kit (Macherey-Nagel Inc., PA, USA) in accordance with the manufacturer's instructions. cDNA was synthesized from the extracted RNA using M-MuLV Reverse Transcriptase (New England BioLabs Inc., MA, USA). .. Cloning, plasmid purification and sequence determination PCR amplification was performed on the cDNA.

    Isolation:

    Article Title: Reduction of the therapeutic dose of silencing RNA by packaging it in extracellular vesicles via a pre-microRNA backbone.
    Article Snippet: A small percentage of the short interfering RNA (siRNA) delivered via passive lipid nanoparticles and other delivery vehicles reaches the cytoplasm of cells. .. A small percentage of the short interfering RNA (siRNA) delivered via passive lipid nanoparticles and other delivery vehicles reaches the cytoplasm of cells.

    Article Title: Foot and mouth disease virus undergoes non-progressive replication in mice peritoneal macrophages and induces M1 polarization.
    Article Snippet: Paragraph title: RNA isolation and gene expression ... One microgram of the total RNA from each sample was reverse transcribed into cDNA using Oligo(dT) primer and the M-MuLV Reverse Transcriptase (New England BioLabs, UK) according to the manufacturer's instructions.

    Polymerase Chain Reaction:

    Article Title: Foot and mouth disease virus undergoes non-progressive replication in mice peritoneal macrophages and induces M1 polarization.
    Article Snippet: One microgram of the total RNA from each sample was reverse transcribed into cDNA using Oligo(dT) primer and the M-MuLV Reverse Transcriptase (New England BioLabs, UK) according to the manufacturer's instructions. .. Gene expression analysis was performed by relative qPCR using SYBR Green PCR Master Mix (Applied Biosystems, USA) according to the manufacturer's instructions.

    Article Title: Evolutionary and epidemiological analyses based on spike genes of porcine epidemic diarrhea virus circulating in Thailand in 2008-2015.
    Article Snippet: Briefly, total RNA was extracted from the supernatant using the Nucleospin®RNA Virus kit (Macherey-Nagel Inc., PA, USA) in accordance with the manufacturer's instructions. cDNA was synthesized from the extracted RNA using M-MuLV Reverse Transcriptase (New England BioLabs Inc., MA, USA). .. Cloning, plasmid purification and sequence determination PCR amplification was performed on the cDNA.

    Infection:

    Article Title: Foot and mouth disease virus undergoes non-progressive replication in mice peritoneal macrophages and induces M1 polarization.
    Article Snippet: Total RNA was extracted from FMDV infected and uninfected peritoneal macrophages using Trizol reagent (Ambion, USA) according to the manufacturer's instructions. .. One microgram of the total RNA from each sample was reverse transcribed into cDNA using Oligo(dT) primer and the M-MuLV Reverse Transcriptase (New England BioLabs, UK) according to the manufacturer's instructions.

    Purification:

    Article Title: Foot and mouth disease virus undergoes non-progressive replication in mice peritoneal macrophages and induces M1 polarization.
    Article Snippet: The purified RNA was further treated with DNase1 (Thermo Scientific, USA) for 60 min at 37°C. .. One microgram of the total RNA from each sample was reverse transcribed into cDNA using Oligo(dT) primer and the M-MuLV Reverse Transcriptase (New England BioLabs, UK) according to the manufacturer's instructions.

    Article Title: Evolutionary and epidemiological analyses based on spike genes of porcine epidemic diarrhea virus circulating in Thailand in 2008-2015.
    Article Snippet: Paragraph title: Cloning, plasmid purification and sequence determination ... Briefly, total RNA was extracted from the supernatant using the Nucleospin®RNA Virus kit (Macherey-Nagel Inc., PA, USA) in accordance with the manufacturer's instructions. cDNA was synthesized from the extracted RNA using M-MuLV Reverse Transcriptase (New England BioLabs Inc., MA, USA).

    SYBR Green Assay:

    Article Title: Foot and mouth disease virus undergoes non-progressive replication in mice peritoneal macrophages and induces M1 polarization.
    Article Snippet: One microgram of the total RNA from each sample was reverse transcribed into cDNA using Oligo(dT) primer and the M-MuLV Reverse Transcriptase (New England BioLabs, UK) according to the manufacturer's instructions. .. Gene expression analysis was performed by relative qPCR using SYBR Green PCR Master Mix (Applied Biosystems, USA) according to the manufacturer's instructions.

    Concentration Assay:

    Article Title: Reduction of the therapeutic dose of silencing RNA by packaging it in extracellular vesicles via a pre-microRNA backbone.
    Article Snippet: A small percentage of the short interfering RNA (siRNA) delivered via passive lipid nanoparticles and other delivery vehicles reaches the cytoplasm of cells. .. A small percentage of the short interfering RNA (siRNA) delivered via passive lipid nanoparticles and other delivery vehicles reaches the cytoplasm of cells.

    Quantitative RT-PCR:

    Article Title: Foot and mouth disease virus undergoes non-progressive replication in mice peritoneal macrophages and induces M1 polarization.
    Article Snippet: RNA isolation and gene expression The expression of the cellular genes including markers of macrophage polarization (iNOS and Arg1), cytokines (TNFα, IL12, IL10, IFNα, IFNβ, IFNγ and IFNλ3) and antiviral molecules (PKR, OAS1a, Mx1 and viperin) was analyzed by qRT-PCR. .. One microgram of the total RNA from each sample was reverse transcribed into cDNA using Oligo(dT) primer and the M-MuLV Reverse Transcriptase (New England BioLabs, UK) according to the manufacturer's instructions.

    Expressing:

    Article Title: Foot and mouth disease virus undergoes non-progressive replication in mice peritoneal macrophages and induces M1 polarization.
    Article Snippet: Paragraph title: RNA isolation and gene expression ... One microgram of the total RNA from each sample was reverse transcribed into cDNA using Oligo(dT) primer and the M-MuLV Reverse Transcriptase (New England BioLabs, UK) according to the manufacturer's instructions.

    Sequencing:

    Article Title: Evolutionary and epidemiological analyses based on spike genes of porcine epidemic diarrhea virus circulating in Thailand in 2008-2015.
    Article Snippet: Paragraph title: Cloning, plasmid purification and sequence determination ... Briefly, total RNA was extracted from the supernatant using the Nucleospin®RNA Virus kit (Macherey-Nagel Inc., PA, USA) in accordance with the manufacturer's instructions. cDNA was synthesized from the extracted RNA using M-MuLV Reverse Transcriptase (New England BioLabs Inc., MA, USA).

    Real-time Polymerase Chain Reaction:

    Article Title: Reduction of the therapeutic dose of silencing RNA by packaging it in extracellular vesicles via a pre-microRNA backbone.
    Article Snippet: A small percentage of the short interfering RNA (siRNA) delivered via passive lipid nanoparticles and other delivery vehicles reaches the cytoplasm of cells. .. A small percentage of the short interfering RNA (siRNA) delivered via passive lipid nanoparticles and other delivery vehicles reaches the cytoplasm of cells.

    Article Title: Foot and mouth disease virus undergoes non-progressive replication in mice peritoneal macrophages and induces M1 polarization.
    Article Snippet: One microgram of the total RNA from each sample was reverse transcribed into cDNA using Oligo(dT) primer and the M-MuLV Reverse Transcriptase (New England BioLabs, UK) according to the manufacturer's instructions. .. Gene expression analysis was performed by relative qPCR using SYBR Green PCR Master Mix (Applied Biosystems, USA) according to the manufacturer's instructions.

    Fluorescence In Situ Hybridization:

    Article Title: Reduction of the therapeutic dose of silencing RNA by packaging it in extracellular vesicles via a pre-microRNA backbone.
    Article Snippet: A small percentage of the short interfering RNA (siRNA) delivered via passive lipid nanoparticles and other delivery vehicles reaches the cytoplasm of cells. .. A small percentage of the short interfering RNA (siRNA) delivered via passive lipid nanoparticles and other delivery vehicles reaches the cytoplasm of cells.

    Transformation Assay:

    Article Title: Evolutionary and epidemiological analyses based on spike genes of porcine epidemic diarrhea virus circulating in Thailand in 2008-2015.
    Article Snippet: Briefly, total RNA was extracted from the supernatant using the Nucleospin®RNA Virus kit (Macherey-Nagel Inc., PA, USA) in accordance with the manufacturer's instructions. cDNA was synthesized from the extracted RNA using M-MuLV Reverse Transcriptase (New England BioLabs Inc., MA, USA). .. The PCR products were cloned into plasmid vectors for the subsequent transformation of Escherichia coli cells by using a commercial kit (pGEM-T® Easy Vector System I (Promega, WI, USA), and the controls were included at all stages of cloning and transformation.

    Plasmid Preparation:

    Article Title: Evolutionary and epidemiological analyses based on spike genes of porcine epidemic diarrhea virus circulating in Thailand in 2008-2015.
    Article Snippet: Paragraph title: Cloning, plasmid purification and sequence determination ... Briefly, total RNA was extracted from the supernatant using the Nucleospin®RNA Virus kit (Macherey-Nagel Inc., PA, USA) in accordance with the manufacturer's instructions. cDNA was synthesized from the extracted RNA using M-MuLV Reverse Transcriptase (New England BioLabs Inc., MA, USA).

    Software:

    Article Title: Reduction of the therapeutic dose of silencing RNA by packaging it in extracellular vesicles via a pre-microRNA backbone.
    Article Snippet: A small percentage of the short interfering RNA (siRNA) delivered via passive lipid nanoparticles and other delivery vehicles reaches the cytoplasm of cells. .. A small percentage of the short interfering RNA (siRNA) delivered via passive lipid nanoparticles and other delivery vehicles reaches the cytoplasm of cells.

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    New England Biolabs mmlv rt
    Reverse transcriptase assays. A. Fluorogenic assay. RT activity was measured by detection of RNA:DNA heteroduplex by fluorescence of EvaGreen binding. Oligo dT primed poly A was incubated at 37°C and 65°C in the presence of indicated Pol enzymes in manufacturer recommended buffers and dTTP. Fluorescence measurements were obtained every 6 seconds for 10 minutes. The initial slopes from a plot of RFU vs. time in seconds were determined by linear least square regression from 30 to 150 seconds at 37°C and from 30 to 90 seconds at 65°C. Error bars are standard error of regression slope. B. RT primer extension assay. HEX-labeled dT 20 primed poly A was incubated 10 minutes at 37°C and then 10 minutes at 65°C in the presence of indicated Pol enzymes and dTTP in manufacturer recommended buffers. Primer extension products were resolved by 10% denaturing PAGE and imaged on a Molecular Imager FX (Bio-Rad). Left facing triangle indicates migration of unextended dT20 primer and asterisk indicates bromophenol blue dye front. C. RT MS2-specific primer extension. 5′-labeled primer was annealed to MS2 RNA and incubated 10 minutes at 37°C and then 30 minutes at 65°C in the presence of indicated Pol enzymes with dNTPS (N = A,C,G,T) in manufacturer recommended buffers. Primer extension products were resolved by 5% denaturing PAGE. Lane 1 No <t>RNA+MMLV</t> RT; Lane 2: MS2 RNA No RT; Lane 3 MS2 RNA+MMLV RT, Lane 3 MS2 <t>RNA+3173</t> Pol. Molecular weight in bases indicted. Red Arrow: ∼650 base MMLV extension product. Blue Arrow: ∼715 base PyroScript extension product. Green arrow: Non-templated MMLV reaction product.
    Mmlv Rt, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mmlv rt/product/New England Biolabs
    Average 99 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    mmlv rt - by Bioz Stars, 2020-04
    99/100 stars
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    Reverse transcriptase assays. A. Fluorogenic assay. RT activity was measured by detection of RNA:DNA heteroduplex by fluorescence of EvaGreen binding. Oligo dT primed poly A was incubated at 37°C and 65°C in the presence of indicated Pol enzymes in manufacturer recommended buffers and dTTP. Fluorescence measurements were obtained every 6 seconds for 10 minutes. The initial slopes from a plot of RFU vs. time in seconds were determined by linear least square regression from 30 to 150 seconds at 37°C and from 30 to 90 seconds at 65°C. Error bars are standard error of regression slope. B. RT primer extension assay. HEX-labeled dT 20 primed poly A was incubated 10 minutes at 37°C and then 10 minutes at 65°C in the presence of indicated Pol enzymes and dTTP in manufacturer recommended buffers. Primer extension products were resolved by 10% denaturing PAGE and imaged on a Molecular Imager FX (Bio-Rad). Left facing triangle indicates migration of unextended dT20 primer and asterisk indicates bromophenol blue dye front. C. RT MS2-specific primer extension. 5′-labeled primer was annealed to MS2 RNA and incubated 10 minutes at 37°C and then 30 minutes at 65°C in the presence of indicated Pol enzymes with dNTPS (N = A,C,G,T) in manufacturer recommended buffers. Primer extension products were resolved by 5% denaturing PAGE. Lane 1 No RNA+MMLV RT; Lane 2: MS2 RNA No RT; Lane 3 MS2 RNA+MMLV RT, Lane 3 MS2 RNA+3173 Pol. Molecular weight in bases indicted. Red Arrow: ∼650 base MMLV extension product. Blue Arrow: ∼715 base PyroScript extension product. Green arrow: Non-templated MMLV reaction product.

    Journal: PLoS ONE

    Article Title: Thermostable DNA Polymerase from a Viral Metagenome Is a Potent RT-PCR Enzyme

    doi: 10.1371/journal.pone.0038371

    Figure Lengend Snippet: Reverse transcriptase assays. A. Fluorogenic assay. RT activity was measured by detection of RNA:DNA heteroduplex by fluorescence of EvaGreen binding. Oligo dT primed poly A was incubated at 37°C and 65°C in the presence of indicated Pol enzymes in manufacturer recommended buffers and dTTP. Fluorescence measurements were obtained every 6 seconds for 10 minutes. The initial slopes from a plot of RFU vs. time in seconds were determined by linear least square regression from 30 to 150 seconds at 37°C and from 30 to 90 seconds at 65°C. Error bars are standard error of regression slope. B. RT primer extension assay. HEX-labeled dT 20 primed poly A was incubated 10 minutes at 37°C and then 10 minutes at 65°C in the presence of indicated Pol enzymes and dTTP in manufacturer recommended buffers. Primer extension products were resolved by 10% denaturing PAGE and imaged on a Molecular Imager FX (Bio-Rad). Left facing triangle indicates migration of unextended dT20 primer and asterisk indicates bromophenol blue dye front. C. RT MS2-specific primer extension. 5′-labeled primer was annealed to MS2 RNA and incubated 10 minutes at 37°C and then 30 minutes at 65°C in the presence of indicated Pol enzymes with dNTPS (N = A,C,G,T) in manufacturer recommended buffers. Primer extension products were resolved by 5% denaturing PAGE. Lane 1 No RNA+MMLV RT; Lane 2: MS2 RNA No RT; Lane 3 MS2 RNA+MMLV RT, Lane 3 MS2 RNA+3173 Pol. Molecular weight in bases indicted. Red Arrow: ∼650 base MMLV extension product. Blue Arrow: ∼715 base PyroScript extension product. Green arrow: Non-templated MMLV reaction product.

    Article Snippet: Two-step RT-PCR reactions were performed using either 5 units 3173 Pol, exonuclease negative mutant or 200 Units MMLV RT (NEB).

    Techniques: Activity Assay, Fluorescence, Binding Assay, Incubation, Primer Extension Assay, Labeling, Polyacrylamide Gel Electrophoresis, Migration, Molecular Weight

    RT-PCR detection of human transcript RNAs. Beta-actin, beta2-microglobulin and cyclophilin target sequences of the indicated sizes were amplified from human liver total RNA using the primers described in Table 1 . Shown are products of two step reactions where either MMLV RT or 3173 Pol were used for first strand cDNA synthesis, as indicated. Taq Pol was used for PCR. Products were resolved on a 1% agarose gel.

    Journal: PLoS ONE

    Article Title: Thermostable DNA Polymerase from a Viral Metagenome Is a Potent RT-PCR Enzyme

    doi: 10.1371/journal.pone.0038371

    Figure Lengend Snippet: RT-PCR detection of human transcript RNAs. Beta-actin, beta2-microglobulin and cyclophilin target sequences of the indicated sizes were amplified from human liver total RNA using the primers described in Table 1 . Shown are products of two step reactions where either MMLV RT or 3173 Pol were used for first strand cDNA synthesis, as indicated. Taq Pol was used for PCR. Products were resolved on a 1% agarose gel.

    Article Snippet: Two-step RT-PCR reactions were performed using either 5 units 3173 Pol, exonuclease negative mutant or 200 Units MMLV RT (NEB).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification, Polymerase Chain Reaction, Agarose Gel Electrophoresis

    Two step RT-PCR comparing 3173 Pol and MMLV RT. A. MS2 viral RNA and B., C. total human liver RNA were reverse transcribed using either 3173 Pol or MMLV RT and then PCR amplified using Taq Polymerase. Target amplicons : A. MS2 RNA phage 77 bp amplicon, 2% gel, B. Human beta-actin 144 bp amplicon, 2% gel, C. Human beta-actin 821 bp amplicon, 1% gel. Lanes : 1 , 11 : oligo dT primer; 2–4 , 12–14 : Gene specific primers; 5 , 15 : random hexamers; 6 , 16 : random nonamers; 7 , 17 : No primer plus RT; 8 : No RT enzyme; 9 : PCR No Target Control; 10 : Molecular Weight Marker (MW), 100 bp (50 bp lowest) for Panels A, B and 1000 bp (300, 500, 700 lowest) for Panel C. Correct PCR product size indicated by black triangle.

    Journal: PLoS ONE

    Article Title: Thermostable DNA Polymerase from a Viral Metagenome Is a Potent RT-PCR Enzyme

    doi: 10.1371/journal.pone.0038371

    Figure Lengend Snippet: Two step RT-PCR comparing 3173 Pol and MMLV RT. A. MS2 viral RNA and B., C. total human liver RNA were reverse transcribed using either 3173 Pol or MMLV RT and then PCR amplified using Taq Polymerase. Target amplicons : A. MS2 RNA phage 77 bp amplicon, 2% gel, B. Human beta-actin 144 bp amplicon, 2% gel, C. Human beta-actin 821 bp amplicon, 1% gel. Lanes : 1 , 11 : oligo dT primer; 2–4 , 12–14 : Gene specific primers; 5 , 15 : random hexamers; 6 , 16 : random nonamers; 7 , 17 : No primer plus RT; 8 : No RT enzyme; 9 : PCR No Target Control; 10 : Molecular Weight Marker (MW), 100 bp (50 bp lowest) for Panels A, B and 1000 bp (300, 500, 700 lowest) for Panel C. Correct PCR product size indicated by black triangle.

    Article Snippet: Two-step RT-PCR reactions were performed using either 5 units 3173 Pol, exonuclease negative mutant or 200 Units MMLV RT (NEB).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Amplification, Molecular Weight, Marker

    Reverse transcription using AHP dUTP. ( A ) RNA template T5 with primer P3. ( B and C ) Twenty percent denaturing PAGE analysis of reactions using AMV and M-MuLV (RNase H-) reverse transcriptases at 42°C for 15 h.

    Journal: Nucleic Acids Research

    Article Title: Efficient enzymatic synthesis and dual-colour fluorescent labelling of DNA probes using long chain azido-dUTP and BCN dyes

    doi: 10.1093/nar/gkw028

    Figure Lengend Snippet: Reverse transcription using AHP dUTP. ( A ) RNA template T5 with primer P3. ( B and C ) Twenty percent denaturing PAGE analysis of reactions using AMV and M-MuLV (RNase H-) reverse transcriptases at 42°C for 15 h.

    Article Snippet: Klenow large fragment, Therminator™ II, M-MuLV (RNase H− ) reverse transcriptase, AMV reverse transcriptase, RNase inhibitor and λ-exonuclease were purchased from New England Biolabs.

    Techniques: Polyacrylamide Gel Electrophoresis