proprietary m extorquens atcc 55366  (ATCC)


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    ATCC proprietary m extorquens atcc 55366
    Utilization of various fatty acids, unsaturated or saturated, for growth and PHA accumulation by M. extorquens ATCC 55366.
    Proprietary M Extorquens Atcc 55366, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Production of functionalized polyhydroxyalkanoates by genetically modified Methylobacterium extorquens strains"

    Article Title: Production of functionalized polyhydroxyalkanoates by genetically modified Methylobacterium extorquens strains

    Journal: Microbial Cell Factories

    doi: 10.1186/1475-2859-9-70


    Figure Legend Snippet: Utilization of various fatty acids, unsaturated or saturated, for growth and PHA accumulation by M. extorquens ATCC 55366.

    Techniques Used: Produced

    m extorquens  (ATCC)


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    ATCC m extorquens
    M Extorquens, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    m extorquens  (ATCC)


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    ATCC m extorquens
    ( a ) A photograph of the cultured solution after culturing M. <t>extorquens</t> in GP media for 7 days; ( b ) photographs of an MMS agar plate streaked with the cultured solution of M. extorquens and incubated at 30 °C for 7 days.
    M Extorquens, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Growth Suppression of a Robust Bacterium Methylobacterium extorquens by Porous Materials with Oxygen Functional Groups"

    Article Title: Growth Suppression of a Robust Bacterium Methylobacterium extorquens by Porous Materials with Oxygen Functional Groups

    Journal: Life

    doi: 10.3390/life13112185

    ( a ) A photograph of the cultured solution after culturing M. extorquens in GP media for 7 days; ( b ) photographs of an MMS agar plate streaked with the cultured solution of M. extorquens and incubated at 30 °C for 7 days.
    Figure Legend Snippet: ( a ) A photograph of the cultured solution after culturing M. extorquens in GP media for 7 days; ( b ) photographs of an MMS agar plate streaked with the cultured solution of M. extorquens and incubated at 30 °C for 7 days.

    Techniques Used: Cell Culture, Incubation

    Photographs of the cultured solutions after culturing M. extorquens in GP liquid medium for 7 days with the porous materials.
    Figure Legend Snippet: Photographs of the cultured solutions after culturing M. extorquens in GP liquid medium for 7 days with the porous materials.

    Techniques Used: Cell Culture

    methylobacterium strains m extorquens pa1  (ATCC)


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    ATCC methylobacterium strains m extorquens pa1
    Strains and plasmids used in this study.
    Methylobacterium Strains M Extorquens Pa1, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Characterization of C 30 carotenoid and identification of its biosynthetic gene cluster in Methylobacterium extorquens AM1"

    Article Title: Characterization of C 30 carotenoid and identification of its biosynthetic gene cluster in Methylobacterium extorquens AM1

    Journal: Synthetic and Systems Biotechnology

    doi: 10.1016/j.synbio.2023.08.002

    Strains and plasmids used in this study.
    Figure Legend Snippet: Strains and plasmids used in this study.

    Techniques Used: Plasmid Preparation, Expressing

    HPLC analysis (A), mass spectrophotometry (MS) analysis (B), and structure (C) of the carotenoids extracted from M. extorquens AM1. (A) The HPLC profile of the metabolites produced by M. extorquens AM1. The compound 1 with retention time at 11.6 min was isolated for further NMR analyses; (B) The mass of compound 1 , [M − H] - = 885.5521 (observed), [M − H] - = 885.5517 (calculated); (C) the structure of compound 1 .
    Figure Legend Snippet: HPLC analysis (A), mass spectrophotometry (MS) analysis (B), and structure (C) of the carotenoids extracted from M. extorquens AM1. (A) The HPLC profile of the metabolites produced by M. extorquens AM1. The compound 1 with retention time at 11.6 min was isolated for further NMR analyses; (B) The mass of compound 1 , [M − H] - = 885.5521 (observed), [M − H] - = 885.5517 (calculated); (C) the structure of compound 1 .

    Techniques Used: Spectrophotometry, Produced, Isolation

    The relative titer of 1 in M. extorquens AM1 derivative strains. YA is M. extorquens AM1 harboring empty pCM80, which is used as control strain. YCM1815, YCM1816, and YCM1815-16 are M. extorquens AM1 derivative strains which carry plasmids for overexpressing the genes META1p1815 , META1p1816 , META1p1815 and META1p1816 , respectively. YCM3665-70 is M. extorquens AM1 derivative strain which carries plasmid for overexpressing genes META1p3665 and META1p3670 . YCM1816-3665-70 is M. extorquens AM1 derivative strain which carries the plasmid for overexpressing the cassette of META1p1816 , META1p3665 and META1p3670 .
    Figure Legend Snippet: The relative titer of 1 in M. extorquens AM1 derivative strains. YA is M. extorquens AM1 harboring empty pCM80, which is used as control strain. YCM1815, YCM1816, and YCM1815-16 are M. extorquens AM1 derivative strains which carry plasmids for overexpressing the genes META1p1815 , META1p1816 , META1p1815 and META1p1816 , respectively. YCM3665-70 is M. extorquens AM1 derivative strain which carries plasmid for overexpressing genes META1p3665 and META1p3670 . YCM1816-3665-70 is M. extorquens AM1 derivative strain which carries the plasmid for overexpressing the cassette of META1p1816 , META1p3665 and META1p3670 .

    Techniques Used: Plasmid Preparation

    Generating META1p3663 deletion mutant strain YA3663 and analyzing its metabolites. (A) Verification of genotype of YA3663 by using PCR, lane M: DNA ladder marker, lane 1: PCR fragments (2106 bp) from M. extorquens AM1 wild type, lane 2: PCR fragments (3267 bp) from mutant strain YA3663. (B) HPLC analyses of metabolites from YA3663, M. extorquens AM1 wild type, and YCM3663. (C) LC-HR-MS analyses of compound 2 generated by strain YA3663. The observed mass of compound 2 is [M − H] - = 459.2541, [2M − H] - = 919.5324, the calculated mass is [M − H] - = 459.2535. (D) The proposed structure of compound 2 based on mass and biosynthetic pathway.
    Figure Legend Snippet: Generating META1p3663 deletion mutant strain YA3663 and analyzing its metabolites. (A) Verification of genotype of YA3663 by using PCR, lane M: DNA ladder marker, lane 1: PCR fragments (2106 bp) from M. extorquens AM1 wild type, lane 2: PCR fragments (3267 bp) from mutant strain YA3663. (B) HPLC analyses of metabolites from YA3663, M. extorquens AM1 wild type, and YCM3663. (C) LC-HR-MS analyses of compound 2 generated by strain YA3663. The observed mass of compound 2 is [M − H] - = 459.2541, [2M − H] - = 919.5324, the calculated mass is [M − H] - = 459.2535. (D) The proposed structure of compound 2 based on mass and biosynthetic pathway.

    Techniques Used: Mutagenesis, Marker, Generated

    Generating META1p3664 deletion mutant strain YA3664 and analyzing its metabolites. (A) Verification of the genotype of the mutant strain YA3664 by using PCR, lane M: DNA ladder marker, lane 1: PCR fragments (2830 bp) from M. extorquens AM1 wild type, lane 2: PCR fragments (2014 bp) from mutant strain YA3664. (B) HPLC analyses of the metabolites from YA3664, M. extorquens AM1 wild type, and YCM3664 (YA3664 complemented with META1p3664 ). (C) LC-HR-MS analyses of compound 3 generated by mutant strain YA3664. The observed mass of compound 3 is [M − H] - = 621.3110, the calculated mass is [M − H] - = 621.3064. (D) The proposed structure of compound 3 based on mass and biosynthetic pathway analyses.
    Figure Legend Snippet: Generating META1p3664 deletion mutant strain YA3664 and analyzing its metabolites. (A) Verification of the genotype of the mutant strain YA3664 by using PCR, lane M: DNA ladder marker, lane 1: PCR fragments (2830 bp) from M. extorquens AM1 wild type, lane 2: PCR fragments (2014 bp) from mutant strain YA3664. (B) HPLC analyses of the metabolites from YA3664, M. extorquens AM1 wild type, and YCM3664 (YA3664 complemented with META1p3664 ). (C) LC-HR-MS analyses of compound 3 generated by mutant strain YA3664. The observed mass of compound 3 is [M − H] - = 621.3110, the calculated mass is [M − H] - = 621.3064. (D) The proposed structure of compound 3 based on mass and biosynthetic pathway analyses.

    Techniques Used: Mutagenesis, Marker, Generated

    The gene cassettes containing META1p3663 (encoding glycosyltransferase), META1p3664 (acyl transferase) and META1p3665 ( crtP ) were identified from Methylobacterium strains which produce C 30 carotenoids with characterized structures.
    Figure Legend Snippet: The gene cassettes containing META1p3663 (encoding glycosyltransferase), META1p3664 (acyl transferase) and META1p3665 ( crtP ) were identified from Methylobacterium strains which produce C 30 carotenoids with characterized structures.

    Techniques Used:

    m extorquen am1  (ATCC)


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    ATCC m extorquen am1
    M Extorquen Am1, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    proprietary m extorquens atcc 55366  (ATCC)


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    ATCC proprietary m extorquens atcc 55366
    Utilization of various fatty acids, unsaturated or saturated, for growth and PHA accumulation by M. extorquens ATCC 55366.
    Proprietary M Extorquens Atcc 55366, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Production of functionalized polyhydroxyalkanoates by genetically modified Methylobacterium extorquens strains"

    Article Title: Production of functionalized polyhydroxyalkanoates by genetically modified Methylobacterium extorquens strains

    Journal: Microbial Cell Factories

    doi: 10.1186/1475-2859-9-70


    Figure Legend Snippet: Utilization of various fatty acids, unsaturated or saturated, for growth and PHA accumulation by M. extorquens ATCC 55366.

    Techniques Used: Produced

    m extorquens atcc 55366 strain  (ATCC)


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    ATCC m extorquens atcc 55366 strain
    Utilization of various fatty acids, unsaturated or saturated, for growth and PHA accumulation by M. extorquens ATCC 55366.
    M Extorquens Atcc 55366 Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Production of functionalized polyhydroxyalkanoates by genetically modified Methylobacterium extorquens strains"

    Article Title: Production of functionalized polyhydroxyalkanoates by genetically modified Methylobacterium extorquens strains

    Journal: Microbial Cell Factories

    doi: 10.1186/1475-2859-9-70


    Figure Legend Snippet: Utilization of various fatty acids, unsaturated or saturated, for growth and PHA accumulation by M. extorquens ATCC 55366.

    Techniques Used: Produced

    pink facultative methylotroph m extorquens atcc 55366  (ATCC)


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    ATCC pink facultative methylotroph m extorquens atcc 55366
    Utilization of various fatty acids, unsaturated or saturated, for growth and PHA accumulation by M. extorquens ATCC 55366.
    Pink Facultative Methylotroph M Extorquens Atcc 55366, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Production of functionalized polyhydroxyalkanoates by genetically modified Methylobacterium extorquens strains"

    Article Title: Production of functionalized polyhydroxyalkanoates by genetically modified Methylobacterium extorquens strains

    Journal: Microbial Cell Factories

    doi: 10.1186/1475-2859-9-70


    Figure Legend Snippet: Utilization of various fatty acids, unsaturated or saturated, for growth and PHA accumulation by M. extorquens ATCC 55366.

    Techniques Used: Produced

    m extorquens atcc 55366 strain  (ATCC)


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    ATCC m extorquens atcc 55366 strain
    Utilization of various fatty acids, unsaturated or saturated, for growth and PHA accumulation by M. extorquens ATCC 55366.
    M Extorquens Atcc 55366 Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Production of functionalized polyhydroxyalkanoates by genetically modified Methylobacterium extorquens strains"

    Article Title: Production of functionalized polyhydroxyalkanoates by genetically modified Methylobacterium extorquens strains

    Journal: Microbial Cell Factories

    doi: 10.1186/1475-2859-9-70


    Figure Legend Snippet: Utilization of various fatty acids, unsaturated or saturated, for growth and PHA accumulation by M. extorquens ATCC 55366.

    Techniques Used: Produced

    m extorquens strain am1  (ATCC)


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    ATCC m extorquens strain am1
    Ribosomal subunit proteins of <t> M. extorquens strain AM1 </t> detected by MALDI-TOF/MS.
    M Extorquens Strain Am1, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "High-Throughput Identification and Screening of Novel Methylobacterium Species Using Whole-Cell MALDI-TOF/MS Analysis"

    Article Title: High-Throughput Identification and Screening of Novel Methylobacterium Species Using Whole-Cell MALDI-TOF/MS Analysis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0040784

    Ribosomal subunit proteins of  M. extorquens strain AM1  detected by MALDI-TOF/MS.
    Figure Legend Snippet: Ribosomal subunit proteins of M. extorquens strain AM1 detected by MALDI-TOF/MS.

    Techniques Used: Methylation

    m extorquens  (ATCC)


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    ATCC m extorquens
    M Extorquens, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC proprietary m extorquens atcc 55366
    Utilization of various fatty acids, unsaturated or saturated, for growth and PHA accumulation by M. extorquens ATCC 55366.
    Proprietary M Extorquens Atcc 55366, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC m extorquens
    Utilization of various fatty acids, unsaturated or saturated, for growth and PHA accumulation by M. extorquens ATCC 55366.
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    ATCC methylobacterium strains m extorquens pa1
    Strains and plasmids used in this study.
    Methylobacterium Strains M Extorquens Pa1, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC m extorquen am1
    Strains and plasmids used in this study.
    M Extorquen Am1, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC m extorquens atcc 55366 strain
    Utilization of various fatty acids, unsaturated or saturated, for growth and PHA accumulation by M. extorquens ATCC 55366.
    M Extorquens Atcc 55366 Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC pink facultative methylotroph m extorquens atcc 55366
    Utilization of various fatty acids, unsaturated or saturated, for growth and PHA accumulation by M. extorquens ATCC 55366.
    Pink Facultative Methylotroph M Extorquens Atcc 55366, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC m extorquens strain am1
    Ribosomal subunit proteins of <t> M. extorquens strain AM1 </t> detected by MALDI-TOF/MS.
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    Journal: Microbial Cell Factories

    Article Title: Production of functionalized polyhydroxyalkanoates by genetically modified Methylobacterium extorquens strains

    doi: 10.1186/1475-2859-9-70

    Figure Lengend Snippet: Utilization of various fatty acids, unsaturated or saturated, for growth and PHA accumulation by M. extorquens ATCC 55366.

    Article Snippet: Our proprietary M. extorquens ATCC 55366 was found unable to yield functionalized PHAs when fed methanol and selected unsaturated carboxylic acids as secondary substrates.

    Techniques: Produced

    Strains and plasmids used in this study.

    Journal: Synthetic and Systems Biotechnology

    Article Title: Characterization of C 30 carotenoid and identification of its biosynthetic gene cluster in Methylobacterium extorquens AM1

    doi: 10.1016/j.synbio.2023.08.002

    Figure Lengend Snippet: Strains and plasmids used in this study.

    Article Snippet: Bioinformatics analyses revealed that the three genes cassette containing META1p3663 , META1p3664 and META1p3665 located in the genomes of Methylobacterium strains M. extorquens PA1, M. populi BJ001, M. radiotolerans JCM2831 and M. rhodinum ATCC 14821 ( ).

    Techniques: Plasmid Preparation, Expressing

    HPLC analysis (A), mass spectrophotometry (MS) analysis (B), and structure (C) of the carotenoids extracted from M. extorquens AM1. (A) The HPLC profile of the metabolites produced by M. extorquens AM1. The compound 1 with retention time at 11.6 min was isolated for further NMR analyses; (B) The mass of compound 1 , [M − H] - = 885.5521 (observed), [M − H] - = 885.5517 (calculated); (C) the structure of compound 1 .

    Journal: Synthetic and Systems Biotechnology

    Article Title: Characterization of C 30 carotenoid and identification of its biosynthetic gene cluster in Methylobacterium extorquens AM1

    doi: 10.1016/j.synbio.2023.08.002

    Figure Lengend Snippet: HPLC analysis (A), mass spectrophotometry (MS) analysis (B), and structure (C) of the carotenoids extracted from M. extorquens AM1. (A) The HPLC profile of the metabolites produced by M. extorquens AM1. The compound 1 with retention time at 11.6 min was isolated for further NMR analyses; (B) The mass of compound 1 , [M − H] - = 885.5521 (observed), [M − H] - = 885.5517 (calculated); (C) the structure of compound 1 .

    Article Snippet: Bioinformatics analyses revealed that the three genes cassette containing META1p3663 , META1p3664 and META1p3665 located in the genomes of Methylobacterium strains M. extorquens PA1, M. populi BJ001, M. radiotolerans JCM2831 and M. rhodinum ATCC 14821 ( ).

    Techniques: Spectrophotometry, Produced, Isolation

    The relative titer of 1 in M. extorquens AM1 derivative strains. YA is M. extorquens AM1 harboring empty pCM80, which is used as control strain. YCM1815, YCM1816, and YCM1815-16 are M. extorquens AM1 derivative strains which carry plasmids for overexpressing the genes META1p1815 , META1p1816 , META1p1815 and META1p1816 , respectively. YCM3665-70 is M. extorquens AM1 derivative strain which carries plasmid for overexpressing genes META1p3665 and META1p3670 . YCM1816-3665-70 is M. extorquens AM1 derivative strain which carries the plasmid for overexpressing the cassette of META1p1816 , META1p3665 and META1p3670 .

    Journal: Synthetic and Systems Biotechnology

    Article Title: Characterization of C 30 carotenoid and identification of its biosynthetic gene cluster in Methylobacterium extorquens AM1

    doi: 10.1016/j.synbio.2023.08.002

    Figure Lengend Snippet: The relative titer of 1 in M. extorquens AM1 derivative strains. YA is M. extorquens AM1 harboring empty pCM80, which is used as control strain. YCM1815, YCM1816, and YCM1815-16 are M. extorquens AM1 derivative strains which carry plasmids for overexpressing the genes META1p1815 , META1p1816 , META1p1815 and META1p1816 , respectively. YCM3665-70 is M. extorquens AM1 derivative strain which carries plasmid for overexpressing genes META1p3665 and META1p3670 . YCM1816-3665-70 is M. extorquens AM1 derivative strain which carries the plasmid for overexpressing the cassette of META1p1816 , META1p3665 and META1p3670 .

    Article Snippet: Bioinformatics analyses revealed that the three genes cassette containing META1p3663 , META1p3664 and META1p3665 located in the genomes of Methylobacterium strains M. extorquens PA1, M. populi BJ001, M. radiotolerans JCM2831 and M. rhodinum ATCC 14821 ( ).

    Techniques: Plasmid Preparation

    Generating META1p3663 deletion mutant strain YA3663 and analyzing its metabolites. (A) Verification of genotype of YA3663 by using PCR, lane M: DNA ladder marker, lane 1: PCR fragments (2106 bp) from M. extorquens AM1 wild type, lane 2: PCR fragments (3267 bp) from mutant strain YA3663. (B) HPLC analyses of metabolites from YA3663, M. extorquens AM1 wild type, and YCM3663. (C) LC-HR-MS analyses of compound 2 generated by strain YA3663. The observed mass of compound 2 is [M − H] - = 459.2541, [2M − H] - = 919.5324, the calculated mass is [M − H] - = 459.2535. (D) The proposed structure of compound 2 based on mass and biosynthetic pathway.

    Journal: Synthetic and Systems Biotechnology

    Article Title: Characterization of C 30 carotenoid and identification of its biosynthetic gene cluster in Methylobacterium extorquens AM1

    doi: 10.1016/j.synbio.2023.08.002

    Figure Lengend Snippet: Generating META1p3663 deletion mutant strain YA3663 and analyzing its metabolites. (A) Verification of genotype of YA3663 by using PCR, lane M: DNA ladder marker, lane 1: PCR fragments (2106 bp) from M. extorquens AM1 wild type, lane 2: PCR fragments (3267 bp) from mutant strain YA3663. (B) HPLC analyses of metabolites from YA3663, M. extorquens AM1 wild type, and YCM3663. (C) LC-HR-MS analyses of compound 2 generated by strain YA3663. The observed mass of compound 2 is [M − H] - = 459.2541, [2M − H] - = 919.5324, the calculated mass is [M − H] - = 459.2535. (D) The proposed structure of compound 2 based on mass and biosynthetic pathway.

    Article Snippet: Bioinformatics analyses revealed that the three genes cassette containing META1p3663 , META1p3664 and META1p3665 located in the genomes of Methylobacterium strains M. extorquens PA1, M. populi BJ001, M. radiotolerans JCM2831 and M. rhodinum ATCC 14821 ( ).

    Techniques: Mutagenesis, Marker, Generated

    Generating META1p3664 deletion mutant strain YA3664 and analyzing its metabolites. (A) Verification of the genotype of the mutant strain YA3664 by using PCR, lane M: DNA ladder marker, lane 1: PCR fragments (2830 bp) from M. extorquens AM1 wild type, lane 2: PCR fragments (2014 bp) from mutant strain YA3664. (B) HPLC analyses of the metabolites from YA3664, M. extorquens AM1 wild type, and YCM3664 (YA3664 complemented with META1p3664 ). (C) LC-HR-MS analyses of compound 3 generated by mutant strain YA3664. The observed mass of compound 3 is [M − H] - = 621.3110, the calculated mass is [M − H] - = 621.3064. (D) The proposed structure of compound 3 based on mass and biosynthetic pathway analyses.

    Journal: Synthetic and Systems Biotechnology

    Article Title: Characterization of C 30 carotenoid and identification of its biosynthetic gene cluster in Methylobacterium extorquens AM1

    doi: 10.1016/j.synbio.2023.08.002

    Figure Lengend Snippet: Generating META1p3664 deletion mutant strain YA3664 and analyzing its metabolites. (A) Verification of the genotype of the mutant strain YA3664 by using PCR, lane M: DNA ladder marker, lane 1: PCR fragments (2830 bp) from M. extorquens AM1 wild type, lane 2: PCR fragments (2014 bp) from mutant strain YA3664. (B) HPLC analyses of the metabolites from YA3664, M. extorquens AM1 wild type, and YCM3664 (YA3664 complemented with META1p3664 ). (C) LC-HR-MS analyses of compound 3 generated by mutant strain YA3664. The observed mass of compound 3 is [M − H] - = 621.3110, the calculated mass is [M − H] - = 621.3064. (D) The proposed structure of compound 3 based on mass and biosynthetic pathway analyses.

    Article Snippet: Bioinformatics analyses revealed that the three genes cassette containing META1p3663 , META1p3664 and META1p3665 located in the genomes of Methylobacterium strains M. extorquens PA1, M. populi BJ001, M. radiotolerans JCM2831 and M. rhodinum ATCC 14821 ( ).

    Techniques: Mutagenesis, Marker, Generated

    The gene cassettes containing META1p3663 (encoding glycosyltransferase), META1p3664 (acyl transferase) and META1p3665 ( crtP ) were identified from Methylobacterium strains which produce C 30 carotenoids with characterized structures.

    Journal: Synthetic and Systems Biotechnology

    Article Title: Characterization of C 30 carotenoid and identification of its biosynthetic gene cluster in Methylobacterium extorquens AM1

    doi: 10.1016/j.synbio.2023.08.002

    Figure Lengend Snippet: The gene cassettes containing META1p3663 (encoding glycosyltransferase), META1p3664 (acyl transferase) and META1p3665 ( crtP ) were identified from Methylobacterium strains which produce C 30 carotenoids with characterized structures.

    Article Snippet: Bioinformatics analyses revealed that the three genes cassette containing META1p3663 , META1p3664 and META1p3665 located in the genomes of Methylobacterium strains M. extorquens PA1, M. populi BJ001, M. radiotolerans JCM2831 and M. rhodinum ATCC 14821 ( ).

    Techniques:

    Journal: Microbial Cell Factories

    Article Title: Production of functionalized polyhydroxyalkanoates by genetically modified Methylobacterium extorquens strains

    doi: 10.1186/1475-2859-9-70

    Figure Lengend Snippet: Utilization of various fatty acids, unsaturated or saturated, for growth and PHA accumulation by M. extorquens ATCC 55366.

    Article Snippet: It was found that the wildtype M. extorquens ATCC 55366 strain could not produce functionalized PHAs starting from a methanol + unsaturated carboxylic acid mixture.

    Techniques: Produced

    Journal: Microbial Cell Factories

    Article Title: Production of functionalized polyhydroxyalkanoates by genetically modified Methylobacterium extorquens strains

    doi: 10.1186/1475-2859-9-70

    Figure Lengend Snippet: Utilization of various fatty acids, unsaturated or saturated, for growth and PHA accumulation by M. extorquens ATCC 55366.

    Article Snippet: The potential of the pink facultative methylotroph M. extorquens ATCC 55366 was tested to utilize various fatty acids including fatty acids with C-C double bonds.

    Techniques: Produced

    Ribosomal subunit proteins of  M. extorquens strain AM1  detected by MALDI-TOF/MS.

    Journal: PLoS ONE

    Article Title: High-Throughput Identification and Screening of Novel Methylobacterium Species Using Whole-Cell MALDI-TOF/MS Analysis

    doi: 10.1371/journal.pone.0040784

    Figure Lengend Snippet: Ribosomal subunit proteins of M. extorquens strain AM1 detected by MALDI-TOF/MS.

    Article Snippet: To check whether WC-MS patterns change depending on cultivation time, M. extorquens strain AM1 (ATCC 14718) was cultured in liquid and on solid medium of methanol medium and R2A, and the samples were analyzed every day for 2 weeks.

    Techniques: Methylation