source m extorquens atcc 55366 wild type atcc transformants cymr1 modified host strain  (ATCC)


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    ATCC source m extorquens atcc 55366 wild type atcc transformants cymr1 modified host strain
    Strains and plasmids used in this study
    Source M Extorquens Atcc 55366 Wild Type Atcc Transformants Cymr1 Modified Host Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Bestowing Inducibility on the Cloned Methanol Dehydrogenase Promoter (P mxaF ) of Methylobacterium extorquens by Applying Regulatory Elements of Pseudomonas putida F1"

    Article Title: Bestowing Inducibility on the Cloned Methanol Dehydrogenase Promoter (P mxaF ) of Methylobacterium extorquens by Applying Regulatory Elements of Pseudomonas putida F1

    Journal:

    doi: 10.1128/AEM.02002-06

    Strains and plasmids used in this study
    Figure Legend Snippet: Strains and plasmids used in this study

    Techniques Used: Plasmid Preparation, Modification, Expressing, Clone Assay, PCR Cloning, Construct

    source m extorquens strains atcc 55366 wild type atcc cymr1 modified host strain  (ATCC)


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    ATCC source m extorquens strains atcc 55366 wild type atcc cymr1 modified host strain
    Source M Extorquens Strains Atcc 55366 Wild Type Atcc Cymr1 Modified Host Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    source m extorquens strains atcc 55366 wild type atcc bgl one copy integrant  (ATCC)


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    ATCC source m extorquens strains atcc 55366 wild type atcc bgl one copy integrant
    Source M Extorquens Strains Atcc 55366 Wild Type Atcc Bgl One Copy Integrant, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    source m extorquens atcc 55366 wild type atcc transformants cymr1 modified host strain  (ATCC)


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    ATCC source m extorquens atcc 55366 wild type atcc transformants cymr1 modified host strain
    Strains and plasmids used in this study
    Source M Extorquens Atcc 55366 Wild Type Atcc Transformants Cymr1 Modified Host Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/source m extorquens atcc 55366 wild type atcc transformants cymr1 modified host strain/product/ATCC
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    94/100 stars

    Images

    1) Product Images from "Bestowing Inducibility on the Cloned Methanol Dehydrogenase Promoter (P mxaF ) of Methylobacterium extorquens by Applying Regulatory Elements of Pseudomonas putida F1"

    Article Title: Bestowing Inducibility on the Cloned Methanol Dehydrogenase Promoter (P mxaF ) of Methylobacterium extorquens by Applying Regulatory Elements of Pseudomonas putida F1

    Journal:

    doi: 10.1128/AEM.02002-06

    Strains and plasmids used in this study
    Figure Legend Snippet: Strains and plasmids used in this study

    Techniques Used: Plasmid Preparation, Modification, Expressing, Clone Assay, PCR Cloning, Construct

    source m extorquens atcc 55366 wild type atcc transformants cymr1 modified host strain  (ATCC)


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    ATCC source m extorquens atcc 55366 wild type atcc transformants cymr1 modified host strain
    Strains and plasmids used in this study
    Source M Extorquens Atcc 55366 Wild Type Atcc Transformants Cymr1 Modified Host Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/source m extorquens atcc 55366 wild type atcc transformants cymr1 modified host strain/product/ATCC
    Average 94 stars, based on 1 article reviews
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    Images

    1) Product Images from "Bestowing Inducibility on the Cloned Methanol Dehydrogenase Promoter (P mxaF ) of Methylobacterium extorquens by Applying Regulatory Elements of Pseudomonas putida F1 "

    Article Title: Bestowing Inducibility on the Cloned Methanol Dehydrogenase Promoter (P mxaF ) of Methylobacterium extorquens by Applying Regulatory Elements of Pseudomonas putida F1

    Journal:

    doi: 10.1128/AEM.02002-06

    Strains and plasmids used in this study
    Figure Legend Snippet: Strains and plasmids used in this study

    Techniques Used: Plasmid Preparation, Modification, Expressing, Clone Assay, PCR Cloning, Construct

    m extorquens type strain  (ATCC)


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    ATCC m extorquens type strain
    Bacterial strains and plasmids used in this study
    M Extorquens Type Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m extorquens type strain/product/ATCC
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    Images

    1) Product Images from "tRNA Is the Source of Low-Level trans- Zeatin Production in Methylobacterium spp."

    Article Title: tRNA Is the Source of Low-Level trans- Zeatin Production in Methylobacterium spp.

    Journal:

    doi: 10.1128/JB.184.7.1832-1842.2002

    Bacterial strains and plasmids used in this study
    Figure Legend Snippet: Bacterial strains and plasmids used in this study

    Techniques Used: Plasmid Preparation

    Amino acid alignment for isopentenyl tRNA transferase (MiaA) from A. tumefaciens (18) and R. prowazekii (2). Partially degenerate primers based on the amino acid sequences located above the dotted lines were constructed based on known codon usage in M. extorquens AM1.
    Figure Legend Snippet: Amino acid alignment for isopentenyl tRNA transferase (MiaA) from A. tumefaciens (18) and R. prowazekii (2). Partially degenerate primers based on the amino acid sequences located above the dotted lines were constructed based on known codon usage in M. extorquens AM1.

    Techniques Used: Construct

    Cytokinins secreted into liquid cultures by a soil isolate of M. extorquens (A) and by plant leaf PPFM isolates obtained from maize (B), soybean (C), and barley (D). The retention times of trans-zeatin (Z) and trans-zeatin riboside (ZR) standards are indicated by bars. The retention times varied due to changes in HPLC gradient conditions resulting from a change in equipment. (B and C) Averages based on triplicate determinations; (A and D) averages based on duplicate determinations. The values were not corrected for recovery.
    Figure Legend Snippet: Cytokinins secreted into liquid cultures by a soil isolate of M. extorquens (A) and by plant leaf PPFM isolates obtained from maize (B), soybean (C), and barley (D). The retention times of trans-zeatin (Z) and trans-zeatin riboside (ZR) standards are indicated by bars. The retention times varied due to changes in HPLC gradient conditions resulting from a change in equipment. (B and C) Averages based on triplicate determinations; (A and D) averages based on duplicate determinations. The values were not corrected for recovery.

    Techniques Used:

    Cytokinins recovered from supernatants of free-living PPFM cultures
    Figure Legend Snippet: Cytokinins recovered from supernatants of free-living PPFM cultures

    Techniques Used:

    RIA of the M. extorquens tRNA hydrolysate purified by anti-cis-zeatin immunoaffinity. The values are averages based on two separate determinations. Twenty percent of the total tRNA hydrolysate was used for analysis. The expected retention time of cis-zeatin (cZR) is indicated by a bar.
    Figure Legend Snippet: RIA of the M. extorquens tRNA hydrolysate purified by anti-cis-zeatin immunoaffinity. The values are averages based on two separate determinations. Twenty percent of the total tRNA hydrolysate was used for analysis. The expected retention time of cis-zeatin (cZR) is indicated by a bar.

    Techniques Used: Purification

    HPLC analysis showing the absence of trans-zeatin riboside (tZR) in tRNA of miaA mutants of M. extorquens. The position of trans-zeatin riboside isolated from wild-type M. extorquens tRNA is indicated in panel A, while a corresponding peak is not present for the mutant in panel B. Ev, electron potential.
    Figure Legend Snippet: HPLC analysis showing the absence of trans-zeatin riboside (tZR) in tRNA of miaA mutants of M. extorquens. The position of trans-zeatin riboside isolated from wild-type M. extorquens tRNA is indicated in panel A, while a corresponding peak is not present for the mutant in panel B. Ev, electron potential.

    Techniques Used: Isolation, Mutagenesis

    HPLC analysis showing the absence of trans-zeatin (tZ) and trans-zeatin riboside (tZR) in the medium of an miaA mutant of M. extorquens. Peaks corresponding to trans isomers isolated from wild-type M. extorquens medium are indicated in panel A, while corresponding peaks are not present for the mutant in panel B. Ev, electron potential.
    Figure Legend Snippet: HPLC analysis showing the absence of trans-zeatin (tZ) and trans-zeatin riboside (tZR) in the medium of an miaA mutant of M. extorquens. Peaks corresponding to trans isomers isolated from wild-type M. extorquens medium are indicated in panel A, while corresponding peaks are not present for the mutant in panel B. Ev, electron potential.

    Techniques Used: Mutagenesis, Isolation

    m extorquens type strain  (ATCC)


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    ATCC m extorquens type strain
    Bacterial strains and plasmids used in this study
    M Extorquens Type Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m extorquens type strain/product/ATCC
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    Images

    1) Product Images from "tRNA Is the Source of Low-Level trans- Zeatin Production in Methylobacterium spp. "

    Article Title: tRNA Is the Source of Low-Level trans- Zeatin Production in Methylobacterium spp.

    Journal:

    doi: 10.1128/JB.184.7.1832-1842.2002

    Bacterial strains and plasmids used in this study
    Figure Legend Snippet: Bacterial strains and plasmids used in this study

    Techniques Used: Plasmid Preparation

    Amino acid alignment for isopentenyl tRNA transferase (MiaA) from A. tumefaciens (18) and R. prowazekii (2). Partially degenerate primers based on the amino acid sequences located above the dotted lines were constructed based on known codon usage in M. extorquens AM1.
    Figure Legend Snippet: Amino acid alignment for isopentenyl tRNA transferase (MiaA) from A. tumefaciens (18) and R. prowazekii (2). Partially degenerate primers based on the amino acid sequences located above the dotted lines were constructed based on known codon usage in M. extorquens AM1.

    Techniques Used: Construct

    Cytokinins secreted into liquid cultures by a soil isolate of M. extorquens (A) and by plant leaf PPFM isolates obtained from maize (B), soybean (C), and barley (D). The retention times of trans-zeatin (Z) and trans-zeatin riboside (ZR) standards are indicated by bars. The retention times varied due to changes in HPLC gradient conditions resulting from a change in equipment. (B and C) Averages based on triplicate determinations; (A and D) averages based on duplicate determinations. The values were not corrected for recovery.
    Figure Legend Snippet: Cytokinins secreted into liquid cultures by a soil isolate of M. extorquens (A) and by plant leaf PPFM isolates obtained from maize (B), soybean (C), and barley (D). The retention times of trans-zeatin (Z) and trans-zeatin riboside (ZR) standards are indicated by bars. The retention times varied due to changes in HPLC gradient conditions resulting from a change in equipment. (B and C) Averages based on triplicate determinations; (A and D) averages based on duplicate determinations. The values were not corrected for recovery.

    Techniques Used:

    Cytokinins recovered from supernatants of free-living PPFM cultures
    Figure Legend Snippet: Cytokinins recovered from supernatants of free-living PPFM cultures

    Techniques Used:

    RIA of the M. extorquens tRNA hydrolysate purified by anti-cis-zeatin immunoaffinity. The values are averages based on two separate determinations. Twenty percent of the total tRNA hydrolysate was used for analysis. The expected retention time of cis-zeatin (cZR) is indicated by a bar.
    Figure Legend Snippet: RIA of the M. extorquens tRNA hydrolysate purified by anti-cis-zeatin immunoaffinity. The values are averages based on two separate determinations. Twenty percent of the total tRNA hydrolysate was used for analysis. The expected retention time of cis-zeatin (cZR) is indicated by a bar.

    Techniques Used: Purification

    HPLC analysis showing the absence of trans-zeatin riboside (tZR) in tRNA of miaA mutants of M. extorquens. The position of trans-zeatin riboside isolated from wild-type M. extorquens tRNA is indicated in panel A, while a corresponding peak is not present for the mutant in panel B. Ev, electron potential.
    Figure Legend Snippet: HPLC analysis showing the absence of trans-zeatin riboside (tZR) in tRNA of miaA mutants of M. extorquens. The position of trans-zeatin riboside isolated from wild-type M. extorquens tRNA is indicated in panel A, while a corresponding peak is not present for the mutant in panel B. Ev, electron potential.

    Techniques Used: Isolation, Mutagenesis

    HPLC analysis showing the absence of trans-zeatin (tZ) and trans-zeatin riboside (tZR) in the medium of an miaA mutant of M. extorquens. Peaks corresponding to trans isomers isolated from wild-type M. extorquens medium are indicated in panel A, while corresponding peaks are not present for the mutant in panel B. Ev, electron potential.
    Figure Legend Snippet: HPLC analysis showing the absence of trans-zeatin (tZ) and trans-zeatin riboside (tZR) in the medium of an miaA mutant of M. extorquens. Peaks corresponding to trans isomers isolated from wild-type M. extorquens medium are indicated in panel A, while corresponding peaks are not present for the mutant in panel B. Ev, electron potential.

    Techniques Used: Mutagenesis, Isolation

    m extorquens type strain  (ATCC)


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    ATCC m extorquens type strain
    M Extorquens Type Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC source m extorquens atcc 55366 wild type atcc transformants cymr1 modified host strain
    Strains and plasmids used in this study
    Source M Extorquens Atcc 55366 Wild Type Atcc Transformants Cymr1 Modified Host Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    ATCC source m extorquens strains atcc 55366 wild type atcc cymr1 modified host strain
    Strains and plasmids used in this study
    Source M Extorquens Strains Atcc 55366 Wild Type Atcc Cymr1 Modified Host Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC source m extorquens strains atcc 55366 wild type atcc bgl one copy integrant
    Strains and plasmids used in this study
    Source M Extorquens Strains Atcc 55366 Wild Type Atcc Bgl One Copy Integrant, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/source m extorquens strains atcc 55366 wild type atcc bgl one copy integrant/product/ATCC
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    ATCC m extorquens type strain
    Bacterial strains and plasmids used in this study
    M Extorquens Type Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Strains and plasmids used in this study

    Journal:

    Article Title: Bestowing Inducibility on the Cloned Methanol Dehydrogenase Promoter (P mxaF ) of Methylobacterium extorquens by Applying Regulatory Elements of Pseudomonas putida F1

    doi: 10.1128/AEM.02002-06

    Figure Lengend Snippet: Strains and plasmids used in this study

    Article Snippet: Antibiotics were used for E. coli and M. extorquens at the following concentrations (in μg/ml): ampicillin, 100; kanamycin (Km), 40; tetracycline (Tc), 35. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain or plasmid Description Reference(s) or source M. extorquens ATCC 55366 Wild type ATCC Transformants CymR1 Modified host strain containing single copy of cym R expression cassette in the chromosome This study CymR2 Modified host strain containing double copy of cym R expression cassette in the chromosome This study GFP M. extorquens (CymR2) containing pCUM-gfp This study BGL M. extorquens (CymR2) containing pCUM-bgl This study EST M. extorquens (CymR2) containing pCUM-est This study Pseudomonas putida F1 Source of cymR gene in the cym operon 11 , 12 E. coli strains Top10 Strain for cloning and propagating plasmid DNA Invitrogen, Inc. S17-1/ λ ( pir ) Host strain for pUX-BF13 17 Plasmids pBK-miniTn7-ΩSm2 pUC19-based delivery plasmid for a miniTn 7 -Km transposon; Km r Sm r 17 pBRI-tet pUC19-based delivery plasmid for a mini-Tn 7 transposon; Tet r This study pBRI80 pUC19-based delivery plasmid for a mini-Tn 7 transposon containing P mxaF and RBS; Tet r This study pBRI-cymR1 pBRI80 plasmid containing one copy of cym R expression cassette This study pBRI-cymR2 pBRI80 plasmid containing two copies of cym R expression cassettes This study pUX-BF13 R6K replicon-based helper plasmid 17 pCR2.1-TOPO PCR cloning vector Invitrogen, Inc. pCR-cymR pCR2.1-TOPO plasmid containing cym R This study PCR-MDHOP pCR2.1-TOPO plasmid containing P mxaF +operator This study pCR-bgl pCR2.1-TOPO plasmid containing bgl This study pCR-est pCR2.1-TOPO plasmid containing est I This study pCR-gfp pCR2.1-TOPO plasmid containing gfp This study pCM110 Wide-host-range cloning vector containing P mxaF ; Tet r 19 pCHOI3 Wide-host-range cloning vector containing P mxaF ; Km r This study pCUM50 Newly constructed regulative expression vector for M. extorquens (CymR2) This study pCUM-bgl pCUM50 plasmid containing β-galactosidase expression cassette This study pCUM-est pCUM50 plasmid containing esterase expression cassette This study pCUM-gfp pCUM50 plasmid containing GFP expression cassette This study pUC19 Multipurpose cloning vector Invitrogen, Inc. pCESTa est I gene source 9 pEBIG4 bgl gene source 15 pQBI63 gfp gene source Qbiogene, Inc. Open in a separate window Strains and plasmids used in this study Construction of expression hosts.

    Techniques: Plasmid Preparation, Modification, Expressing, Clone Assay, PCR Cloning, Construct

    Bacterial strains and plasmids used in this study

    Journal:

    Article Title: tRNA Is the Source of Low-Level trans- Zeatin Production in Methylobacterium spp.

    doi: 10.1128/JB.184.7.1832-1842.2002

    Figure Lengend Snippet: Bacterial strains and plasmids used in this study

    Article Snippet: The M. extorquens type strain (ATCC 43645) was obtained from the American Type Culture Collection; this strain is a soil isolate that was described by Urakami and Komagata ( 45 ) and Bousfield and Green ( 5 ).

    Techniques: Plasmid Preparation

    Amino acid alignment for isopentenyl tRNA transferase (MiaA) from A. tumefaciens (18) and R. prowazekii (2). Partially degenerate primers based on the amino acid sequences located above the dotted lines were constructed based on known codon usage in M. extorquens AM1.

    Journal:

    Article Title: tRNA Is the Source of Low-Level trans- Zeatin Production in Methylobacterium spp.

    doi: 10.1128/JB.184.7.1832-1842.2002

    Figure Lengend Snippet: Amino acid alignment for isopentenyl tRNA transferase (MiaA) from A. tumefaciens (18) and R. prowazekii (2). Partially degenerate primers based on the amino acid sequences located above the dotted lines were constructed based on known codon usage in M. extorquens AM1.

    Article Snippet: The M. extorquens type strain (ATCC 43645) was obtained from the American Type Culture Collection; this strain is a soil isolate that was described by Urakami and Komagata ( 45 ) and Bousfield and Green ( 5 ).

    Techniques: Construct

    Cytokinins secreted into liquid cultures by a soil isolate of M. extorquens (A) and by plant leaf PPFM isolates obtained from maize (B), soybean (C), and barley (D). The retention times of trans-zeatin (Z) and trans-zeatin riboside (ZR) standards are indicated by bars. The retention times varied due to changes in HPLC gradient conditions resulting from a change in equipment. (B and C) Averages based on triplicate determinations; (A and D) averages based on duplicate determinations. The values were not corrected for recovery.

    Journal:

    Article Title: tRNA Is the Source of Low-Level trans- Zeatin Production in Methylobacterium spp.

    doi: 10.1128/JB.184.7.1832-1842.2002

    Figure Lengend Snippet: Cytokinins secreted into liquid cultures by a soil isolate of M. extorquens (A) and by plant leaf PPFM isolates obtained from maize (B), soybean (C), and barley (D). The retention times of trans-zeatin (Z) and trans-zeatin riboside (ZR) standards are indicated by bars. The retention times varied due to changes in HPLC gradient conditions resulting from a change in equipment. (B and C) Averages based on triplicate determinations; (A and D) averages based on duplicate determinations. The values were not corrected for recovery.

    Article Snippet: The M. extorquens type strain (ATCC 43645) was obtained from the American Type Culture Collection; this strain is a soil isolate that was described by Urakami and Komagata ( 45 ) and Bousfield and Green ( 5 ).

    Techniques:

    Cytokinins recovered from supernatants of free-living PPFM cultures

    Journal:

    Article Title: tRNA Is the Source of Low-Level trans- Zeatin Production in Methylobacterium spp.

    doi: 10.1128/JB.184.7.1832-1842.2002

    Figure Lengend Snippet: Cytokinins recovered from supernatants of free-living PPFM cultures

    Article Snippet: The M. extorquens type strain (ATCC 43645) was obtained from the American Type Culture Collection; this strain is a soil isolate that was described by Urakami and Komagata ( 45 ) and Bousfield and Green ( 5 ).

    Techniques:

    RIA of the M. extorquens tRNA hydrolysate purified by anti-cis-zeatin immunoaffinity. The values are averages based on two separate determinations. Twenty percent of the total tRNA hydrolysate was used for analysis. The expected retention time of cis-zeatin (cZR) is indicated by a bar.

    Journal:

    Article Title: tRNA Is the Source of Low-Level trans- Zeatin Production in Methylobacterium spp.

    doi: 10.1128/JB.184.7.1832-1842.2002

    Figure Lengend Snippet: RIA of the M. extorquens tRNA hydrolysate purified by anti-cis-zeatin immunoaffinity. The values are averages based on two separate determinations. Twenty percent of the total tRNA hydrolysate was used for analysis. The expected retention time of cis-zeatin (cZR) is indicated by a bar.

    Article Snippet: The M. extorquens type strain (ATCC 43645) was obtained from the American Type Culture Collection; this strain is a soil isolate that was described by Urakami and Komagata ( 45 ) and Bousfield and Green ( 5 ).

    Techniques: Purification

    HPLC analysis showing the absence of trans-zeatin riboside (tZR) in tRNA of miaA mutants of M. extorquens. The position of trans-zeatin riboside isolated from wild-type M. extorquens tRNA is indicated in panel A, while a corresponding peak is not present for the mutant in panel B. Ev, electron potential.

    Journal:

    Article Title: tRNA Is the Source of Low-Level trans- Zeatin Production in Methylobacterium spp.

    doi: 10.1128/JB.184.7.1832-1842.2002

    Figure Lengend Snippet: HPLC analysis showing the absence of trans-zeatin riboside (tZR) in tRNA of miaA mutants of M. extorquens. The position of trans-zeatin riboside isolated from wild-type M. extorquens tRNA is indicated in panel A, while a corresponding peak is not present for the mutant in panel B. Ev, electron potential.

    Article Snippet: The M. extorquens type strain (ATCC 43645) was obtained from the American Type Culture Collection; this strain is a soil isolate that was described by Urakami and Komagata ( 45 ) and Bousfield and Green ( 5 ).

    Techniques: Isolation, Mutagenesis

    HPLC analysis showing the absence of trans-zeatin (tZ) and trans-zeatin riboside (tZR) in the medium of an miaA mutant of M. extorquens. Peaks corresponding to trans isomers isolated from wild-type M. extorquens medium are indicated in panel A, while corresponding peaks are not present for the mutant in panel B. Ev, electron potential.

    Journal:

    Article Title: tRNA Is the Source of Low-Level trans- Zeatin Production in Methylobacterium spp.

    doi: 10.1128/JB.184.7.1832-1842.2002

    Figure Lengend Snippet: HPLC analysis showing the absence of trans-zeatin (tZ) and trans-zeatin riboside (tZR) in the medium of an miaA mutant of M. extorquens. Peaks corresponding to trans isomers isolated from wild-type M. extorquens medium are indicated in panel A, while corresponding peaks are not present for the mutant in panel B. Ev, electron potential.

    Article Snippet: The M. extorquens type strain (ATCC 43645) was obtained from the American Type Culture Collection; this strain is a soil isolate that was described by Urakami and Komagata ( 45 ) and Bousfield and Green ( 5 ).

    Techniques: Mutagenesis, Isolation