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Millipore m edta
Lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P) induce Ca 2+ mobilization and phospholipase D (PLD) activation in type II alveolar epithelial cells. (a, c) A549 cells were labelled with 3 μ m Fluo-3/AM, treated or not with <t>EDTA,</t> <t>EGTA</t>
M Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 163 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m edta/product/Millipore
Average 92 stars, based on 163 article reviews
Price from $9.99 to $1999.99
m edta - by Bioz Stars, 2020-07
92/100 stars

Images

1) Product Images from "Natural lysophospholipids reduce Mycobacterium tuberculosis-induced cytotoxicity and induce anti-mycobacterial activity by a phagolysosome maturation-dependent mechanism in A549 type II alveolar epithelial cells"

Article Title: Natural lysophospholipids reduce Mycobacterium tuberculosis-induced cytotoxicity and induce anti-mycobacterial activity by a phagolysosome maturation-dependent mechanism in A549 type II alveolar epithelial cells

Journal: Immunology

doi: 10.1111/j.1365-2567.2009.03145.x

Lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P) induce Ca 2+ mobilization and phospholipase D (PLD) activation in type II alveolar epithelial cells. (a, c) A549 cells were labelled with 3 μ m Fluo-3/AM, treated or not with EDTA, EGTA
Figure Legend Snippet: Lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P) induce Ca 2+ mobilization and phospholipase D (PLD) activation in type II alveolar epithelial cells. (a, c) A549 cells were labelled with 3 μ m Fluo-3/AM, treated or not with EDTA, EGTA

Techniques Used: Activation Assay

2) Product Images from "Interacting Regions of CD81 and Two of Its Partners, EWI-2 and EWI-2wint, and Their Effect on Hepatitis C Virus Infection *"

Article Title: Interacting Regions of CD81 and Two of Its Partners, EWI-2 and EWI-2wint, and Their Effect on Hepatitis C Virus Infection *

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M111.220103

Mutations in EWI-2/EWI-2wint that abolish interaction with CD81 also affect the inhibitory effect of EWI-2wint on HCV infection. A , Huh-7 cell clones expressing the different FLAG-tagged mutants of EWI-2/EWI-2wint were biotinylated, lysed in PBS/Brij97/EDTA,
Figure Legend Snippet: Mutations in EWI-2/EWI-2wint that abolish interaction with CD81 also affect the inhibitory effect of EWI-2wint on HCV infection. A , Huh-7 cell clones expressing the different FLAG-tagged mutants of EWI-2/EWI-2wint were biotinylated, lysed in PBS/Brij97/EDTA,

Techniques Used: Infection, Clone Assay, Expressing

3) Product Images from "Cathepsin L Is Responsible for Processing and Activation of Proheparanase through Multiple Cleavages of a Linker Segment *"

Article Title: Cathepsin L Is Responsible for Processing and Activation of Proheparanase through Multiple Cleavages of a Linker Segment *

Journal:

doi: 10.1074/jbc.M801327200

Cathepsin L knock-out ( KO ) fibroblasts and tissues are unable to process proheparanase. Fibroblasts derived from either cathepsin L knock-out RT2 tumors (○) or wild-type RT2 tumors (•) were lysed (1% Nonidet P-40, 10 m m EDTA in PBS
Figure Legend Snippet: Cathepsin L knock-out ( KO ) fibroblasts and tissues are unable to process proheparanase. Fibroblasts derived from either cathepsin L knock-out RT2 tumors (○) or wild-type RT2 tumors (•) were lysed (1% Nonidet P-40, 10 m m EDTA in PBS

Techniques Used: Knock-Out, Derivative Assay

4) Product Images from "Tau Protein Assembles into Isoform- and Disulfide-dependent Polymorphic Fibrils with Distinct Structural Properties *"

Article Title: Tau Protein Assembles into Isoform- and Disulfide-dependent Polymorphic Fibrils with Distinct Structural Properties *

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M111.248963

A seeding reaction of Tau fibrils monitored by ThT fluorescence intensity. 10 μ m Tau of 1N3R CA ( A ), 1N3R S-S ( B ), 1N4R CA ( C ), and 1N4R S-S ( D ) was prepared in a 50 m m Tris buffer, pH 7.2, containing 1 m m EDTA, 2.5 μ m heparin, and 16.7 μ
Figure Legend Snippet: A seeding reaction of Tau fibrils monitored by ThT fluorescence intensity. 10 μ m Tau of 1N3R CA ( A ), 1N3R S-S ( B ), 1N4R CA ( C ), and 1N4R S-S ( D ) was prepared in a 50 m m Tris buffer, pH 7.2, containing 1 m m EDTA, 2.5 μ m heparin, and 16.7 μ

Techniques Used: Fluorescence

Tau fibrils with alternative structural properties are emerged by a seeding reaction. A , a seeding reaction of 10 μ m 1N3R CA in a 50 m m Tris buffer, pH 7.2, containing 1 m m EDTA, 2.5 μ m heparin, and 16.7 μ m ThT is monitored by a
Figure Legend Snippet: Tau fibrils with alternative structural properties are emerged by a seeding reaction. A , a seeding reaction of 10 μ m 1N3R CA in a 50 m m Tris buffer, pH 7.2, containing 1 m m EDTA, 2.5 μ m heparin, and 16.7 μ m ThT is monitored by a

Techniques Used:

5) Product Images from "DNA Sequence Context as a Determinant of the Quantity and Chemistry of Guanine Oxidation Produced by Hydroxyl Radicals and One-electron Oxidants *DNA Sequence Context as a Determinant of the Quantity and Chemistry of Guanine Oxidation Produced by Hydroxyl Radicals and One-electron Oxidants * S⃞"

Article Title: DNA Sequence Context as a Determinant of the Quantity and Chemistry of Guanine Oxidation Produced by Hydroxyl Radicals and One-electron Oxidants *DNA Sequence Context as a Determinant of the Quantity and Chemistry of Guanine Oxidation Produced by Hydroxyl Radicals and One-electron Oxidants * S⃞

Journal:

doi: 10.1074/jbc.M806809200

Ratios of relative amounts of Fpg- to piperidine-sensitive oxidized G lesions produced by Fe 2+ -EDTA/H 2 O 2 ( white bars ) and γ-radiation ( black bars ) ( A ) and riboflavin-mediated photooxidation ( white bars ) and ( black bars ; data from Ref.
Figure Legend Snippet: Ratios of relative amounts of Fpg- to piperidine-sensitive oxidized G lesions produced by Fe 2+ -EDTA/H 2 O 2 ( white bars ) and γ-radiation ( black bars ) ( A ) and riboflavin-mediated photooxidation ( white bars ) and ( black bars ; data from Ref.

Techniques Used: Produced

6) Product Images from "Molecular Characterization of Arylsulfatase G"

Article Title: Molecular Characterization of Arylsulfatase G

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M114.584144

Lysosomal cysteine proteases cathepsin B and cathepsin L mediate the processing of the ARSG precursor. A, [ 35 S]methionine/cysteine pulse-chase analysis of stably mArsg transfected WT MEFs exposed to a mixture of different protease inhibitors and various inhibitors reveals impaired processing in E64D (cysteine protease inhibitor)- and leupeptin ( Leupep. ) (a general lysosomal inhibitor)-treated cells. Other inhibitors now show changes compared with vehicle-treated cells ((4-(2-aminoethyl)benzenesulfonyl fluoride ( AEBSF ), pepstatin ( Pepst. ), and EDTA) or completely abolished processing (phenanthroline ( Phenant. )). B, stably mArsg-transfected MEFs derived from mice deficient for cathepsin B ( CtsB −/− ), cathepsin L ( CtsL −/− ), cathepsin B and L ( CtsB/L −/− ), cathepsin Z ( CtsZ ), cathepsin H ( CtsH −/− ), asparagine endopeptidase ( AEP −/− ), and cathepsin D ( CtsD −/− ) were analyzed by immunoblotting and showed impaired ARSG processing in CtsB/L −/− double-deficient cells. C, Western blot of brain homogenates shows reduced levels of the processed 34-kDa ARSG polypeptide band in double-deficient CtsB/L mice on the endogenous level.
Figure Legend Snippet: Lysosomal cysteine proteases cathepsin B and cathepsin L mediate the processing of the ARSG precursor. A, [ 35 S]methionine/cysteine pulse-chase analysis of stably mArsg transfected WT MEFs exposed to a mixture of different protease inhibitors and various inhibitors reveals impaired processing in E64D (cysteine protease inhibitor)- and leupeptin ( Leupep. ) (a general lysosomal inhibitor)-treated cells. Other inhibitors now show changes compared with vehicle-treated cells ((4-(2-aminoethyl)benzenesulfonyl fluoride ( AEBSF ), pepstatin ( Pepst. ), and EDTA) or completely abolished processing (phenanthroline ( Phenant. )). B, stably mArsg-transfected MEFs derived from mice deficient for cathepsin B ( CtsB −/− ), cathepsin L ( CtsL −/− ), cathepsin B and L ( CtsB/L −/− ), cathepsin Z ( CtsZ ), cathepsin H ( CtsH −/− ), asparagine endopeptidase ( AEP −/− ), and cathepsin D ( CtsD −/− ) were analyzed by immunoblotting and showed impaired ARSG processing in CtsB/L −/− double-deficient cells. C, Western blot of brain homogenates shows reduced levels of the processed 34-kDa ARSG polypeptide band in double-deficient CtsB/L mice on the endogenous level.

Techniques Used: Pulse Chase, Stable Transfection, Transfection, Protease Inhibitor, Derivative Assay, Mouse Assay, Western Blot

Related Articles

Protease Inhibitor:

Article Title: Molecular Characterization of Arylsulfatase G
Article Snippet: .. Inhibitor concentrations were 1 m m 4-(2-aminoethyl)benzenesulfonyl fluoride, 40 μ m E-64d, 100 μ m pepstatin A, 100 μ m leupeptin, 5 m m EDTA, 5 m m 1,10-phenanthroline, and a commercial protease inhibitor mixture according to the manufacturer's recommendations (Sigma). ..

Article Title: Adenomatous Polyposis Coli (APC) Regulates Multiple Signaling Pathways by Enhancing Glycogen Synthase Kinase-3 (GSK-3) Activity *
Article Snippet: .. Cells were lysed in buffer containing 20 m m Tris pH 7.5, 140 m m NaCl, 1 m m EDTA, 10% glycerol, 1% Triton X-100, 1 m m DTT, 50 m m NaF, and protease inhibitor mixture (Sigma P8340), phosphatase inhibitor mixture #1 (Sigma P2850) and #2 (Sigma P5726) or #2 and #3 (Sigma P0044) used 1:100 each. .. Lysis buffer used in contained 20 m m Tris, pH 8.0 instead of pH 7.5.

Incubation:

Article Title: DNA Sequence Context as a Determinant of the Quantity and Chemistry of Guanine Oxidation Produced by Hydroxyl Radicals and One-electron Oxidants *DNA Sequence Context as a Determinant of the Quantity and Chemistry of Guanine Oxidation Produced by Hydroxyl Radicals and One-electron Oxidants * S⃞
Article Snippet: .. To remove ExoIII activity prior to Fpg reactions, oligodeoxynucleotides were incubated with 20 m m EDTA to chelate Mg2+ present in 1× NE buffer 1 and passed through protein-binding Micropure-EZ filters (Millipore, Bedford, MA). ..

other:

Article Title: Proteasomal Degradation of Eukaryotic Elongation Factor-2 Kinase (EF2K) Is Regulated by cAMP-PKA Signaling and the SCFβTRCP Ubiquitin E3 Ligase *
Article Snippet: Cells were stimulated with either forskolin (Tocris, 10 μ m in DMSO), IGF-1 (Millipore, 100 ng/ml in water), CGS 21680 hydrochloride (Tocris, 100 n m in DMSO), or 6-BNZ-cAMP (Sigma, 100 μ m in water) for up to 48 h and lysed in buffer containing 1% Nonidet P-40, 50 m m Tris, pH 7.4, 200 m m NaCl, 1 m m EDTA, and protease and phosphatase inhibitor cocktails (Sigma, 1:100).

Activity Assay:

Article Title: DNA Sequence Context as a Determinant of the Quantity and Chemistry of Guanine Oxidation Produced by Hydroxyl Radicals and One-electron Oxidants *DNA Sequence Context as a Determinant of the Quantity and Chemistry of Guanine Oxidation Produced by Hydroxyl Radicals and One-electron Oxidants * S⃞
Article Snippet: .. To remove ExoIII activity prior to Fpg reactions, oligodeoxynucleotides were incubated with 20 m m EDTA to chelate Mg2+ present in 1× NE buffer 1 and passed through protein-binding Micropure-EZ filters (Millipore, Bedford, MA). ..

Protein Binding:

Article Title: DNA Sequence Context as a Determinant of the Quantity and Chemistry of Guanine Oxidation Produced by Hydroxyl Radicals and One-electron Oxidants *DNA Sequence Context as a Determinant of the Quantity and Chemistry of Guanine Oxidation Produced by Hydroxyl Radicals and One-electron Oxidants * S⃞
Article Snippet: .. To remove ExoIII activity prior to Fpg reactions, oligodeoxynucleotides were incubated with 20 m m EDTA to chelate Mg2+ present in 1× NE buffer 1 and passed through protein-binding Micropure-EZ filters (Millipore, Bedford, MA). ..

Western Blot:

Article Title: Cathepsin L Is Responsible for Processing and Activation of Proheparanase through Multiple Cleavages of a Linker Segment *
Article Snippet: .. SDS-PAGE and Western Blot Analysis —Cells (1 × 106 ) were lysed in buffer containing 1% Brij 35, 150 m m NaCl, 50 m m Tris-HCl, pH 7.5, or in buffer containing 1% Nonidet P-40, 10 m m EDTA in PBS, both supplemented with a mixture of protease inhibitors (Sigma) ( ). .. Cells were incubated with the lysis buffer for 15–30 min on ice, cell debris were removed by centrifugation and the protein concentration in the supernatants was determined using the Bradford protein assay (Bio-Rad).

SDS Page:

Article Title: Cathepsin L Is Responsible for Processing and Activation of Proheparanase through Multiple Cleavages of a Linker Segment *
Article Snippet: .. SDS-PAGE and Western Blot Analysis —Cells (1 × 106 ) were lysed in buffer containing 1% Brij 35, 150 m m NaCl, 50 m m Tris-HCl, pH 7.5, or in buffer containing 1% Nonidet P-40, 10 m m EDTA in PBS, both supplemented with a mixture of protease inhibitors (Sigma) ( ). .. Cells were incubated with the lysis buffer for 15–30 min on ice, cell debris were removed by centrifugation and the protein concentration in the supernatants was determined using the Bradford protein assay (Bio-Rad).

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    Millipore m edta
    Lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P) induce Ca 2+ mobilization and phospholipase D (PLD) activation in type II alveolar epithelial cells. (a, c) A549 cells were labelled with 3 μ m Fluo-3/AM, treated or not with <t>EDTA,</t> <t>EGTA</t>
    M Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 163 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m edta/product/Millipore
    Average 92 stars, based on 163 article reviews
    Price from $9.99 to $1999.99
    m edta - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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    Lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P) induce Ca 2+ mobilization and phospholipase D (PLD) activation in type II alveolar epithelial cells. (a, c) A549 cells were labelled with 3 μ m Fluo-3/AM, treated or not with EDTA, EGTA

    Journal: Immunology

    Article Title: Natural lysophospholipids reduce Mycobacterium tuberculosis-induced cytotoxicity and induce anti-mycobacterial activity by a phagolysosome maturation-dependent mechanism in A549 type II alveolar epithelial cells

    doi: 10.1111/j.1365-2567.2009.03145.x

    Figure Lengend Snippet: Lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P) induce Ca 2+ mobilization and phospholipase D (PLD) activation in type II alveolar epithelial cells. (a, c) A549 cells were labelled with 3 μ m Fluo-3/AM, treated or not with EDTA, EGTA

    Article Snippet: In several experiments, 2 m m EDTA or 3 m m EGTA (Calbiochem) were added 15 min before the addition of LPA or S1P, whereas 20 μ m 1,2-bis(2-aminophenoxy)ethane-N,N,N1,N1-tetraacetic acid tetraicis (acetoxymethyl ester) (BAPTA-AM) (Sigma-Aldrich, Milan, Italy) was added 30 min before stimulation with LPA or S1P.

    Techniques: Activation Assay

    Ratios of relative amounts of Fpg- to piperidine-sensitive oxidized G lesions produced by Fe 2+ -EDTA/H 2 O 2 ( white bars ) and γ-radiation ( black bars ) ( A ) and riboflavin-mediated photooxidation ( white bars ) and ( black bars ; data from Ref.

    Journal:

    Article Title: DNA Sequence Context as a Determinant of the Quantity and Chemistry of Guanine Oxidation Produced by Hydroxyl Radicals and One-electron Oxidants *DNA Sequence Context as a Determinant of the Quantity and Chemistry of Guanine Oxidation Produced by Hydroxyl Radicals and One-electron Oxidants * S⃞

    doi: 10.1074/jbc.M806809200

    Figure Lengend Snippet: Ratios of relative amounts of Fpg- to piperidine-sensitive oxidized G lesions produced by Fe 2+ -EDTA/H 2 O 2 ( white bars ) and γ-radiation ( black bars ) ( A ) and riboflavin-mediated photooxidation ( white bars ) and ( black bars ; data from Ref.

    Article Snippet: To remove ExoIII activity prior to Fpg reactions, oligodeoxynucleotides were incubated with 20 m m EDTA to chelate Mg2+ present in 1× NE buffer 1 and passed through protein-binding Micropure-EZ filters (Millipore, Bedford, MA).

    Techniques: Produced