m dtt  (Roche)


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  • 92

    Structured Review

    Roche m dtt
    M Dtt, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dtt/product/Roche
    Average 92 stars, based on 121 article reviews
    Price from $9.99 to $1999.99
    m dtt - by Bioz Stars, 2020-09
    92/100 stars

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    Related Articles

    Protease Inhibitor:

    Article Title: Aryl Hydrocarbon Receptor-interacting Protein-like 1 Is an Obligate Chaperone of Phosphodiesterase 6 and Is Assisted by the γ-Subunit of Its Client *
    Article Snippet: .. Cell pellets were sonicated on ice (five 30-s pulses) in 50 m m Tris-HCl buffer (pH 8) containing 100 m m NaCl, 50 m m l -arginine, 50 m m l -glutamic acid, 10% glycerol, 10 m m DTT (buffer B), and EDTA-free mini protease inhibitor mixture (Roche Applied Science). .. His6 -tagged AIPL1 protein and mutant forms were purified over nickel-nitrilotriacetic acid resin (Novagen), using buffer B containing 250 m m imidazole for elution.

    Article Title: High Boron-induced Ubiquitination Regulates Vacuolar Sorting of the BOR1 Borate Transporter in Arabidopsis thaliana *
    Article Snippet: .. Microsomes containing 20 μg of protein were dissolved in 2× solubilization buffer (50 m m Tris-Cl (pH 7.5), 50 m m DTT, 5% glycerol, 5% SDS, 5 m m EDTA, 0.03% bromphenol blue, and one tablet/10 ml protease inhibitor mixture (Complete mini, EDTA-free; Roche Applied Science)), incubated at 65 °C for 5 min, and then separated by SDS-PAGE using a modified Laemmli system ( ) with 8% acrylamide and 0.064% N , N ′-methylene-bis-acrylamide. ..

    Article Title: Insights into Herpesvirus Tegument Organization from Structural Analyses of the 970 Central Residues of HSV-1 UL36 Protein
    Article Snippet: .. The pellet was resuspended in 50 m m HEPES, pH 7.0, 500 m m NaCl, 10% glycerol, 1 m m DTT supplemented by a tablet of EDTA-free “Complete” protease inhibitor mixture tablet (Roche Diagnostics) and lysed by sonication at 4 °C. .. The supernatant was recovered and centrifuged at 4 °C for 30 min at 48,000 × g in a JA25.50 rotor (Beckman Coulter).

    Article Title: Molecular Characterization of Plant Ubiquitin-Conjugating Enzymes Belonging to the UbcP4/E2-C/UBCx/UbcH10 Gene Family 1
    Article Snippet: .. The Arabidopsis cells were homogenized using pestle and mortar in extraction buffer (25 m m Tris-HCl, pH 7.5, 15 m m MgCl2 , 15 m m EGTA, 150 m m NaCl, 0.1% [w/v] Tween 20, 1 m m DTT, and complete protease inhibitor cocktail mix [Roche Diagnostics, Indianapolis]) with PolyClar AT (Serva, Garden City Park, NY) and quartz and centrifuged at 20,000 g . .. The protein content was determined by using the Bio-Rad protein assay kit.

    Article Title: Impaired Cyclic Electron Flow around Photosystem I Disturbs High-Light Respiratory Metabolism 1
    Article Snippet: .. Aliquots of 20 mg of frozen-powdered leaf tissue (i.e. from the same frozen-powdered material used for metabolite analysis) were used to extract proteins by adding 100 μL of SDS sample buffer (2% [w/v] SDS, 62.5 m m Tris-HCl [pH 6.8], 10% [v/v] glycerol, and 0.007% [w/v] Bromophenol Blue) as well as 50 m m DTT and a protease inhibitor tablet (Roche). .. Samples were then incubated for 30 min on ice to allow full reduction of the AOX protein.

    Article Title: Intraspinal microstimulation preferentially recruits fatigue-resistant muscle fibres and generates gradual force in rat
    Article Snippet: .. Briefly, muscles were homogenized in ice-cold buffer containing 100 m m Na4 P2 O7 (pH 8.5), 5 m m EGTA, 5 m m MgCl2 , 0.3 m KCl, 10 m m DTT and 5 mg ml−1 of a protease inhibitor cocktail (Complete™, Roche Diagnostics Corporation, Indianapolis, IN, USA). .. Total protein content of the extracts was analysed using the Bio-Rad protein assay (Bio-Rad Laboratories, Hercules, CA, USA) , diluted to 0.2 μg μl−1 in Laemmli-lysis buffer ( ) and boiled for 10 min. Five microlitres of each extract was electrophoresed for 24 h at 275 V and 12°C on 7% polyacrylamide gels containing glycerol, under denaturing conditions.

    Incubation:

    Article Title: High Boron-induced Ubiquitination Regulates Vacuolar Sorting of the BOR1 Borate Transporter in Arabidopsis thaliana *
    Article Snippet: .. Microsomes containing 20 μg of protein were dissolved in 2× solubilization buffer (50 m m Tris-Cl (pH 7.5), 50 m m DTT, 5% glycerol, 5% SDS, 5 m m EDTA, 0.03% bromphenol blue, and one tablet/10 ml protease inhibitor mixture (Complete mini, EDTA-free; Roche Applied Science)), incubated at 65 °C for 5 min, and then separated by SDS-PAGE using a modified Laemmli system ( ) with 8% acrylamide and 0.064% N , N ′-methylene-bis-acrylamide. ..

    Lysis:

    Article Title: Efficient Production of Dual Recombinant Adeno-Associated Viral Vectors for Factor VIII Delivery
    Article Snippet: .. Total protein fraction was extracted with a lysis buffer consisting of 50 m M Tris at pH 8.0, 150 m M NaCl, 1% Triton X-100, 10 m M DTT, and 1× protein inhibitor (Roche). .. Cell lysates (50 μg protein) were resolved on 10% SDS-PAGE gel and transferred to a nitrocellulose membrane (Bio-Rad).

    Modification:

    Article Title: High Boron-induced Ubiquitination Regulates Vacuolar Sorting of the BOR1 Borate Transporter in Arabidopsis thaliana *
    Article Snippet: .. Microsomes containing 20 μg of protein were dissolved in 2× solubilization buffer (50 m m Tris-Cl (pH 7.5), 50 m m DTT, 5% glycerol, 5% SDS, 5 m m EDTA, 0.03% bromphenol blue, and one tablet/10 ml protease inhibitor mixture (Complete mini, EDTA-free; Roche Applied Science)), incubated at 65 °C for 5 min, and then separated by SDS-PAGE using a modified Laemmli system ( ) with 8% acrylamide and 0.064% N , N ′-methylene-bis-acrylamide. ..

    Sonication:

    Article Title: Aryl Hydrocarbon Receptor-interacting Protein-like 1 Is an Obligate Chaperone of Phosphodiesterase 6 and Is Assisted by the γ-Subunit of Its Client *
    Article Snippet: .. Cell pellets were sonicated on ice (five 30-s pulses) in 50 m m Tris-HCl buffer (pH 8) containing 100 m m NaCl, 50 m m l -arginine, 50 m m l -glutamic acid, 10% glycerol, 10 m m DTT (buffer B), and EDTA-free mini protease inhibitor mixture (Roche Applied Science). .. His6 -tagged AIPL1 protein and mutant forms were purified over nickel-nitrilotriacetic acid resin (Novagen), using buffer B containing 250 m m imidazole for elution.

    Article Title: Insights into Herpesvirus Tegument Organization from Structural Analyses of the 970 Central Residues of HSV-1 UL36 Protein
    Article Snippet: .. The pellet was resuspended in 50 m m HEPES, pH 7.0, 500 m m NaCl, 10% glycerol, 1 m m DTT supplemented by a tablet of EDTA-free “Complete” protease inhibitor mixture tablet (Roche Diagnostics) and lysed by sonication at 4 °C. .. The supernatant was recovered and centrifuged at 4 °C for 30 min at 48,000 × g in a JA25.50 rotor (Beckman Coulter).

    SDS Page:

    Article Title: High Boron-induced Ubiquitination Regulates Vacuolar Sorting of the BOR1 Borate Transporter in Arabidopsis thaliana *
    Article Snippet: .. Microsomes containing 20 μg of protein were dissolved in 2× solubilization buffer (50 m m Tris-Cl (pH 7.5), 50 m m DTT, 5% glycerol, 5% SDS, 5 m m EDTA, 0.03% bromphenol blue, and one tablet/10 ml protease inhibitor mixture (Complete mini, EDTA-free; Roche Applied Science)), incubated at 65 °C for 5 min, and then separated by SDS-PAGE using a modified Laemmli system ( ) with 8% acrylamide and 0.064% N , N ′-methylene-bis-acrylamide. ..

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  • m dtt  (Roche)
    92
    Roche m dtt
    Purified CpIscA can acquire an Fe-S cluster. A, Gel filtration analysis. CpIscA (300 μ g) was incubated with CpNifS (75 μ g) in reconstitution buffer (50 m m <t>Tricine-NaOH,</t> pH 7.5, 5 m m <t>DTT,</t> 1 m m l -Cys, 1 m m ferrous ammonium sulfate, 20 μ
    M Dtt, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dtt/product/Roche
    Average 92 stars, based on 121 article reviews
    Price from $9.99 to $1999.99
    m dtt - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    85
    Roche m dithiothreitol buffer
    Removal of Zn 2+ causes loss of structure in parkin. CD spectra of full-length parkin (3 μ m ) in 5 m m Tris, 20 m m NaCl, and 1 m m <t>dithiothreitol</t> at pH 7.4. The spectra show full-length parkin in the absence ( thick solid line ), and presence of
    M Dithiothreitol Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dithiothreitol buffer/product/Roche
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m dithiothreitol buffer - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    92
    Roche m dithiothreitol
    Unfolding plots in guanidine chloride of the purified heteropolymers. The ferritins (50 μg/ml) were incubated for 18 h at 4 °C in 0.1 m phosphate buffer, pH 7.4, 1 m m <t>dithiothreitol,</t> with the different guanidine HCl concentrations. Unfolding
    M Dithiothreitol, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dithiothreitol/product/Roche
    Average 92 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    m dithiothreitol - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    Image Search Results


    Purified CpIscA can acquire an Fe-S cluster. A, Gel filtration analysis. CpIscA (300 μ g) was incubated with CpNifS (75 μ g) in reconstitution buffer (50 m m Tricine-NaOH, pH 7.5, 5 m m DTT, 1 m m l -Cys, 1 m m ferrous ammonium sulfate, 20 μ

    Journal:

    Article Title: Iron-Sulfur Cluster Biogenesis in Chloroplasts. Involvement of the Scaffold Protein CpIscA 1

    doi: 10.1104/pp.104.058602

    Figure Lengend Snippet: Purified CpIscA can acquire an Fe-S cluster. A, Gel filtration analysis. CpIscA (300 μ g) was incubated with CpNifS (75 μ g) in reconstitution buffer (50 m m Tricine-NaOH, pH 7.5, 5 m m DTT, 1 m m l -Cys, 1 m m ferrous ammonium sulfate, 20 μ

    Article Snippet: The role of IscA in Fe-S cluster assembly was assayed by incubating IscA (30 μ g) with NifS (7.5 μ g) for 30 min in reconstitution buffer containing 50 m m Tricine-NaOH, pH 7.5, 5 m m DTT (Roche Diagnostics, Palo Alto, CA), 1 m m l -Cys (Sigma, St. Louis), 1 m m ferrous ammonium sulfate, and 20 μ m PLP.

    Techniques: Purification, Filtration, Incubation

    Removal of Zn 2+ causes loss of structure in parkin. CD spectra of full-length parkin (3 μ m ) in 5 m m Tris, 20 m m NaCl, and 1 m m dithiothreitol at pH 7.4. The spectra show full-length parkin in the absence ( thick solid line ), and presence of

    Journal: The Journal of Biological Chemistry

    Article Title: Identification of a Novel Zn2+-binding Domain in the Autosomal Recessive Juvenile Parkinson-related E3 Ligase Parkin *-binding Domain in the Autosomal Recessive Juvenile Parkinson-related E3 Ligase Parkin * S⃞

    doi: 10.1074/jbc.M808700200

    Figure Lengend Snippet: Removal of Zn 2+ causes loss of structure in parkin. CD spectra of full-length parkin (3 μ m ) in 5 m m Tris, 20 m m NaCl, and 1 m m dithiothreitol at pH 7.4. The spectra show full-length parkin in the absence ( thick solid line ), and presence of

    Article Snippet: The E. coli cells were harvested and lysed by sonication on ice in 20 m m Tris-HCl (pH 7.4), 120 m m NaCl, 1 m m dithiothreitol buffer supplemented with EDTA-free protease inhibitor mixture (Roche Applied Science).

    Techniques:

    Unfolding plots in guanidine chloride of the purified heteropolymers. The ferritins (50 μg/ml) were incubated for 18 h at 4 °C in 0.1 m phosphate buffer, pH 7.4, 1 m m dithiothreitol, with the different guanidine HCl concentrations. Unfolding

    Journal: The Journal of Biological Chemistry

    Article Title: Mutant Ferritin L-chains That Cause Neurodegeneration Act in a Dominant-negative Manner to Reduce Ferritin Iron Incorporation *

    doi: 10.1074/jbc.M109.096404

    Figure Lengend Snippet: Unfolding plots in guanidine chloride of the purified heteropolymers. The ferritins (50 μg/ml) were incubated for 18 h at 4 °C in 0.1 m phosphate buffer, pH 7.4, 1 m m dithiothreitol, with the different guanidine HCl concentrations. Unfolding

    Article Snippet: Bands of interest were excised from gels, subjected to reduction by 10 m m dithiothreitol, alkylation by 55 m m iodoacetic acid, and finally digested with trypsin overnight (Roche Applied Science) ( ).

    Techniques: Purification, Incubation