m dtt  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    DTT
    Description:

    Catalog Number:
    ge17-1318-01
    Price:
    None
    Buy from Supplier


    Structured Review

    Millipore m dtt
    DTT

    https://www.bioz.com/result/m dtt/product/Millipore
    Average 99 stars, based on 75 article reviews
    Price from $9.99 to $1999.99
    m dtt - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "An Autoinhibitory Domain Confers Redox Regulation to Maize Glycerate Kinase 1An Autoinhibitory Domain Confers Redox Regulation to Maize Glycerate Kinase 1 [W]"

    Article Title: An Autoinhibitory Domain Confers Redox Regulation to Maize Glycerate Kinase 1An Autoinhibitory Domain Confers Redox Regulation to Maize Glycerate Kinase 1 [W]

    Journal: Plant Physiology

    doi: 10.1104/pp.110.157719

    Redox titration of ZmGLYK activity. Recombinant ZmGLYK was incubated with varying ratios of DTT ox /DTT and 0.9 μ m EcoTrx at pH 7.7 and assayed for activity. Data points represent means from three independent experiments. The redox midpoint potential
    Figure Legend Snippet: Redox titration of ZmGLYK activity. Recombinant ZmGLYK was incubated with varying ratios of DTT ox /DTT and 0.9 μ m EcoTrx at pH 7.7 and assayed for activity. Data points represent means from three independent experiments. The redox midpoint potential

    Techniques Used: Titration, Activity Assay, Recombinant, Incubation

    Light and thiol activation of GLYK in desalted maize leaf soluble crude extracts. A, Light activation after 4 h of dark adaptation of leaves. After 10 min of illumination, leaves were darkened again. B, Thiol activation by addition of 10 m m DTT to extracts
    Figure Legend Snippet: Light and thiol activation of GLYK in desalted maize leaf soluble crude extracts. A, Light activation after 4 h of dark adaptation of leaves. After 10 min of illumination, leaves were darkened again. B, Thiol activation by addition of 10 m m DTT to extracts

    Techniques Used: Activation Assay

    2) Product Images from "Folding of a Cyclin Box"

    Article Title: Folding of a Cyclin Box

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M113.467316

    RbAB particle size distribution at different GdmCl concentrations. Dynamic light scattering measurements of a 10 μ m RbAB solution in 20 m m phosphate buffer, pH 7.0, 200 m m NaCl, and 2 m m DTT with different GdmCl concentrations. The GdmCl concentration
    Figure Legend Snippet: RbAB particle size distribution at different GdmCl concentrations. Dynamic light scattering measurements of a 10 μ m RbAB solution in 20 m m phosphate buffer, pH 7.0, 200 m m NaCl, and 2 m m DTT with different GdmCl concentrations. The GdmCl concentration

    Techniques Used: Concentration Assay

    3) Product Images from "Discovery of a Novel Alginate Lyase from Nitratiruptor sp. SB155-2 Thriving at Deep-sea Hydrothermal Vents and Identification of the Residues Responsible for Its Heat Stability *"

    Article Title: Discovery of a Novel Alginate Lyase from Nitratiruptor sp. SB155-2 Thriving at Deep-sea Hydrothermal Vents and Identification of the Residues Responsible for Its Heat Stability *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M115.713230

    Bacterial expression and purification of rNitAly. A , schematic drawing of rNitAly. B , SDS-PAGE of purified rNitAly. Reduced and non-reduced indicate that the samples were prepared in the presence and absence of 20 m m DTT, respectively. C , SDS-PAGE of
    Figure Legend Snippet: Bacterial expression and purification of rNitAly. A , schematic drawing of rNitAly. B , SDS-PAGE of purified rNitAly. Reduced and non-reduced indicate that the samples were prepared in the presence and absence of 20 m m DTT, respectively. C , SDS-PAGE of

    Techniques Used: Expressing, Purification, SDS Page

    4) Product Images from "Dithiol-based Compounds Maintain Expression of Antioxidant Protein Peroxiredoxin 1 That Counteracts Toxicity of Mutant Huntingtin *"

    Article Title: Dithiol-based Compounds Maintain Expression of Antioxidant Protein Peroxiredoxin 1 That Counteracts Toxicity of Mutant Huntingtin *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M111.334565

    DMP treatment confers protection against mHtt-induced toxicity. PC12–103Q cells were treated with various thiol-based compounds including β-mercaptoethanol ( BME ), DTT, cysteamine ( Cys ), NAC, eeGSH, DMP, DMSA, and penicillimine ( Pen ) for
    Figure Legend Snippet: DMP treatment confers protection against mHtt-induced toxicity. PC12–103Q cells were treated with various thiol-based compounds including β-mercaptoethanol ( BME ), DTT, cysteamine ( Cys ), NAC, eeGSH, DMP, DMSA, and penicillimine ( Pen ) for

    Techniques Used:

    5) Product Images from "Arabidopsis thaliana AMY3 Is a Unique Redox-regulated Chloroplastic α-Amylase *"

    Article Title: Arabidopsis thaliana AMY3 Is a Unique Redox-regulated Chloroplastic α-Amylase *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M113.514794

    Midpoint redox potential of At AMY3 at pH 7. 9. The ambient redox potential ( E h ), adjusted by defined ratios of DTT oxidized /DTT reduced , ranged from −270 to −420 mV. Preoxidized At AMY3 protein (10 μg) was incubated in Tricine/NaOH
    Figure Legend Snippet: Midpoint redox potential of At AMY3 at pH 7. 9. The ambient redox potential ( E h ), adjusted by defined ratios of DTT oxidized /DTT reduced , ranged from −270 to −420 mV. Preoxidized At AMY3 protein (10 μg) was incubated in Tricine/NaOH

    Techniques Used: Incubation

    C587S site-specific mutant of At AMY3 is redox-insensitive. Purified At AMY3 and its cysteine mutant variants (1 μg each) were electrophoresed on native polyacrylamide gels containing amylopectin. Gels were incubated with 5 m m DTT ( A ) or 50 μ
    Figure Legend Snippet: C587S site-specific mutant of At AMY3 is redox-insensitive. Purified At AMY3 and its cysteine mutant variants (1 μg each) were electrophoresed on native polyacrylamide gels containing amylopectin. Gels were incubated with 5 m m DTT ( A ) or 50 μ

    Techniques Used: Mutagenesis, Purification, Incubation

    Redox regulation of AtAMY3 activity. A and B , redox sensitivity of At AMY3 and Hv AMY1. Purified recombinant proteins were exposed to 5 m m DTT or 50 μ m CuCl 2 for 2 h prior to activity assay. Reduced and oxidized proteins were then incubated for
    Figure Legend Snippet: Redox regulation of AtAMY3 activity. A and B , redox sensitivity of At AMY3 and Hv AMY1. Purified recombinant proteins were exposed to 5 m m DTT or 50 μ m CuCl 2 for 2 h prior to activity assay. Reduced and oxidized proteins were then incubated for

    Techniques Used: Activity Assay, Purification, Recombinant, Incubation

    Related Articles

    Centrifugation:

    Article Title: Autophagy participates in the unfolded protein response in Toxoplasma gondii
    Article Snippet: .. Freshly egressed parasites were collected by centrifugation and incubated for 6 hours in Hank's Balanced Salt Solution (HBSS, Gibco), or DMEM in the presence of ER stress-inducing agents: 5 mM dithiothreitol (DTT), 10 μM brefeldin A (BFA) and 10 μg/ml tunicamycin (Tuni) (all purchased from Sigma-Aldrich, Saint Quentin Fallavier, France). .. Parasites were then centrifuged, and the pellet was resuspended in loading buffer before being separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and analyzed by immunoblot.

    other:

    Article Title: A critical cysteine residue in monoacylglycerol lipase is targeted by a new class of isothiazolinone-based enzyme inhibitors
    Article Snippet: Compounds 1, 2, 6–8 and dithiothreitol (DTT) were from Sigma-Aldrich (St. Louis, MO, USA).

    Activity Assay:

    Article Title: Inhibition of Helicobacter pylori and Its Associated Urease by Palmatine: Investigation on the Potential Mechanism
    Article Snippet: .. Acetohydroxamic acid (AHA, CAS number: 546-88-3, purity: 98%), urea (Molecular Biology Reagent), jack bean urease (JBU, type III with specific activity 40.3 U/mg solid), HEPES (Amresco > 99%), L-cysteine (L-cys), glutathione (GSH), dithiothreithol (DTT), boric acid (BA) and sodium fluoride (NaF) were all purchased from Sigma-Aldrich (St Louis, MO, USA). .. Bradford Protein Assay Kit was purchased from Beyotime Institute of Biotechnology (Shanghai, China).

    Article Title: Lipase Activity in the Larval Midgut of Rhynchophorus palmarum: Biochemical Characterization and the Effects of Reducing Agents
    Article Snippet: .. Effects of the Reducing Agents To determine the effects of reducing agents on lipase activity, reduced glutathione (GSH), dithiothreitol (DTT), and oxidized glutathione (GSSG) (Sigma-Aldrich Co, St Louis, MO, USA) were incubated with the posterior midgut tissue homogenates for 30 min with different concentrations of each reagent. ..

    Incubation:

    Article Title: Autophagy participates in the unfolded protein response in Toxoplasma gondii
    Article Snippet: .. Freshly egressed parasites were collected by centrifugation and incubated for 6 hours in Hank's Balanced Salt Solution (HBSS, Gibco), or DMEM in the presence of ER stress-inducing agents: 5 mM dithiothreitol (DTT), 10 μM brefeldin A (BFA) and 10 μg/ml tunicamycin (Tuni) (all purchased from Sigma-Aldrich, Saint Quentin Fallavier, France). .. Parasites were then centrifuged, and the pellet was resuspended in loading buffer before being separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and analyzed by immunoblot.

    Article Title: Lipase Activity in the Larval Midgut of Rhynchophorus palmarum: Biochemical Characterization and the Effects of Reducing Agents
    Article Snippet: .. Effects of the Reducing Agents To determine the effects of reducing agents on lipase activity, reduced glutathione (GSH), dithiothreitol (DTT), and oxidized glutathione (GSSG) (Sigma-Aldrich Co, St Louis, MO, USA) were incubated with the posterior midgut tissue homogenates for 30 min with different concentrations of each reagent. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore m dtt
    DMP treatment confers protection against mHtt-induced toxicity. PC12–103Q cells were treated with various thiol-based compounds including <t>β-mercaptoethanol</t> ( BME ), <t>DTT,</t> cysteamine ( Cys ), NAC, eeGSH, DMP, DMSA, and penicillimine ( Pen ) for
    M Dtt, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dtt/product/Millipore
    Average 99 stars, based on 108 article reviews
    Price from $9.99 to $1999.99
    m dtt - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    DMP treatment confers protection against mHtt-induced toxicity. PC12–103Q cells were treated with various thiol-based compounds including β-mercaptoethanol ( BME ), DTT, cysteamine ( Cys ), NAC, eeGSH, DMP, DMSA, and penicillimine ( Pen ) for

    Journal: The Journal of Biological Chemistry

    Article Title: Dithiol-based Compounds Maintain Expression of Antioxidant Protein Peroxiredoxin 1 That Counteracts Toxicity of Mutant Huntingtin *

    doi: 10.1074/jbc.M111.334565

    Figure Lengend Snippet: DMP treatment confers protection against mHtt-induced toxicity. PC12–103Q cells were treated with various thiol-based compounds including β-mercaptoethanol ( BME ), DTT, cysteamine ( Cys ), NAC, eeGSH, DMP, DMSA, and penicillimine ( Pen ) for

    Article Snippet: Extracts resolved under reducing conditions were treated with 100 m m DTT and 2% β-ME and boiled for 5 min. Gels were electroblotted onto Immobilon-P membrane (Millipore, Bedford, MA), blocked with 1% milk and 3% BSA (Sigma) in Tris-buffered saline with 0.5% Tween 20 (TBST).

    Techniques: