m alpina strain atcc 32222  (ATCC)

Journal: Journal of Fungi

Article Title: Applications of Diacylglycerol Acyltransferase for Triacylglycerol Production in Mortierella alpina

doi: 10.3390/jof9020219

Figure Lengend Snippet: Total biomass and fatty acid yield of M. alpina-MaDGAT1B , M. alpina-MaDGAT2A , and M. alpina ATCC 32222. For each group, three biological replicates were used.

Article Snippet: The uracil-auxotrophic strain M. alpina CCFM 501 was constructed by homologous recombination to knock out the ura5 gene encoding orotate phosphoribosyl transferase (OPRTase, EC.2.4.2.10), based on the wild-type strain M. alpina ATCC 32222.

Techniques:

Journal: Journal of Fungi

Article Title: Applications of Diacylglycerol Acyltransferase for Triacylglycerol Production in Mortierella alpina

doi: 10.3390/jof9020219

Figure Lengend Snippet: Major lipid content in M. alpina ATCC 32222, M. alpina-MaDGAT1B , and M. alpina-MaDGAT2A . ( a ) The content of TG and ( b ) PC, DG, PE, and other lipids. The significant differences are indicated by * p < 0.05, ** p < 0.01, and *** p < 0.001. For each group, three biological replicates were used. TG, triacylglycerol; PC, phosphatidylcholine; DG, diacylglycerol; PE, phosphatidylethanolamine.

Article Snippet: The uracil-auxotrophic strain M. alpina CCFM 501 was constructed by homologous recombination to knock out the ura5 gene encoding orotate phosphoribosyl transferase (OPRTase, EC.2.4.2.10), based on the wild-type strain M. alpina ATCC 32222.

Techniques:

Journal: Journal of Fungi

Article Title: Applications of Diacylglycerol Acyltransferase for Triacylglycerol Production in Mortierella alpina

doi: 10.3390/jof9020219

Figure Lengend Snippet: Lipidomics profiles in M. alpina ATCC 32222, M. alpina-MaDGAT1B , and M. alpina-MaDGAT2A . ( a ) The distribution of TG profiles, ( b ) DG profiles, and ( c ) PC profiles. For each group, three biological replicates were used.

Article Snippet: The uracil-auxotrophic strain M. alpina CCFM 501 was constructed by homologous recombination to knock out the ura5 gene encoding orotate phosphoribosyl transferase (OPRTase, EC.2.4.2.10), based on the wild-type strain M. alpina ATCC 32222.

Techniques:

Journal: Journal of Fungi

Article Title: Applications of Diacylglycerol Acyltransferase for Triacylglycerol Production in Mortierella alpina

doi: 10.3390/jof9020219

Figure Lengend Snippet: Total biomass and total lipid yield of M. alpina ATCC 32222 and M. alpina-MaDGAT2A without LSO and with 0.5 g/L LSO supplementation. For each group, three biological replicates were used. LSO, linseed oil.

Article Snippet: The uracil-auxotrophic strain M. alpina CCFM 501 was constructed by homologous recombination to knock out the ura5 gene encoding orotate phosphoribosyl transferase (OPRTase, EC.2.4.2.10), based on the wild-type strain M. alpina ATCC 32222.

Techniques: Concentration Assay

Journal: Journal of Fungi

Article Title: Applications of Diacylglycerol Acyltransferase for Triacylglycerol Production in Mortierella alpina

doi: 10.3390/jof9020219

Figure Lengend Snippet: Major lipid content in M. alpina ATCC 32222 and M. alpina-MaDGAT2A with or without LSO supplementation. ( a ) The content of TG and ( b ) PC, DG, PE, and other lipids. For each group, three biological replicates were used.

Article Snippet: The uracil-auxotrophic strain M. alpina CCFM 501 was constructed by homologous recombination to knock out the ura5 gene encoding orotate phosphoribosyl transferase (OPRTase, EC.2.4.2.10), based on the wild-type strain M. alpina ATCC 32222.

Techniques:

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Application of a ω-3 Desaturase with an Arachidonic Acid Preference to Eicosapentaenoic Acid Production in Mortierella alpina

doi: 10.3389/fbioe.2017.00089

Figure Lengend Snippet: Construction of the binary vector pBIG2-ura5s- oPaFADS17 . The his 550 promoter fragment was derived from Mortierella alpina ATCC 32222. The trpCt transcription terminator region was derived from Aspergillus nidulans . LB, left border; RB, right border. M, marker; lane 1, M. alpina (negative control); lanes 2–7: M. alpina - oPaFADS17 -(1–6). Bands at 818 and 1,244 bp indicate the presence of the oPaFADS17 gene.

Article Snippet: The wild-type M. alpina ATCC 32222 strain was purchased from American Type Culture Collection (ATCC, Manassas, VA, USA) and conserved in our lab; the M. alpina uracil-auxotrophic strain (CCFM501, Culture Collection of Food Microorganisms, Jiangnan University, Jiangsu Province, PR China) was modified from wild-type M. alpina in our lab (Hao et al., ).

Techniques: Plasmid Preparation, Derivative Assay, Marker, Negative Control