streptavidin coated m 280 dynabeads  (Thermo Fisher)


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    Structured Review

    Thermo Fisher streptavidin coated m 280 dynabeads
    Streptavidin Coated M 280 Dynabeads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin coated m 280 dynabeads/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    streptavidin coated m 280 dynabeads - by Bioz Stars, 2020-05
    99/100 stars

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    Magnetic Beads:

    Article Title: Allele-specific quantitative proteomics unravels molecular mechanisms modulated by cis-regulatory PPARG locus variation
    Article Snippet: .. According to the manufacturer΄s instructions, streptavidin coupled magnetic beads (Dynabeads M-280, Invitrogen, Darmstadt, Germany) were washed and collected using Bind & Wash buffer (Dynabeads M-280, Invitrogen, Darmstadt, Germany) and Magnetic particle separator (Magna-Sep™, Invitrogen, Darmstadt, Germany), discarding the supernatant. .. Magnetic beads were coupled to biotinylated oligonucleotides at 4°C overnight.

    Article Title: MC159 of Molluscum Contagiosum Virus Suppresses Autophagy by Recruiting Cellular SH3BP4 via an SH3 Domain-Mediated Interaction
    Article Snippet: .. For precipitation of the BD fusion proteins, streptavidin-coated magnetic beads (Dynabeads M-280-Streptavidin; Invitrogen, CA, USA) were used according to the manufacturer’s instructions. .. Then the beads were washed once with 1 ml of cold lysis buffer and twice with 1 ml of cold PBST (PBS plus 0,05% Tween 20) for 1 min each wash. For performing the SH3 phage display on the precipitated BD-fusion proteins, the target protein-containing beads were incubated with a mixture of human SH3 library-displaying phages about 1 × 1011 colony forming units (CFU) and 5% milk in PBST at a ratio of 2:1 for 90 min under rotation at room temperature.

    Article Title: A phospho-dependent mechanism involving NCoR and KMT2D controls a permissive chromatin state at Notch target genes
    Article Snippet: .. For Western blot purposes 500 μg of nuclear extract were incubated with streptavidin magnetic beads (Dynabeads M-280, Invitrogen 112.06) in a binding buffer containing 120 mM NaCl and 0.1% NP-40. .. After binding, beads were washed five times with streptavidin washing buffer containing 300 mM NaCl and two times with PBS.

    Article Title: Hepatoma-derived growth factor-related protein 2 promotes DNA repair by homologous recombination
    Article Snippet: .. Histone peptide pulldown Biotin-conjugated histone 3 (H3) peptides (Eurogentech) were immobilized on streptavidin magnetic beads (Life Technologies, Dynabeads M-280 Streptavidin) and incubated with nuclear extracts prepared as in the immunoprecipitation procedure but resolved in binding buffer (150 mM Hepes-KOH (pH7.6), 200 mM KCl, 1 mM MgCl2 , 1 mM EDTA, 10% Glycerol, 0.5% NP-40, 1 mM DTT + Protease Inhibitors) for 2 h at 4°C. .. Bound proteins were washed five times in binding buffer, boiled in 2× SDS sample buffer and analysed by immunoblotting.

    Purification:

    Article Title: TRAP/SMCC/Mediator-Dependent Transcriptional Activation from DNA and Chromatin Templates by Orphan Nuclear Receptor Hepatocyte Nuclear Factor 4
    Article Snippet: .. 600 bp) from plasmid pA4xMLΔ53 ( , ), which contains the HNF-4 cognate sites and the core promoter elements, was filled in with biotinylated dATP by using the Klenow fragment of DNA Pol I, gel purified, and bound to M280-streptavidin Dynabeads (Dynal), as suggested by the manufacturer. ..

    Immunoprecipitation:

    Article Title: Hepatoma-derived growth factor-related protein 2 promotes DNA repair by homologous recombination
    Article Snippet: .. Histone peptide pulldown Biotin-conjugated histone 3 (H3) peptides (Eurogentech) were immobilized on streptavidin magnetic beads (Life Technologies, Dynabeads M-280 Streptavidin) and incubated with nuclear extracts prepared as in the immunoprecipitation procedure but resolved in binding buffer (150 mM Hepes-KOH (pH7.6), 200 mM KCl, 1 mM MgCl2 , 1 mM EDTA, 10% Glycerol, 0.5% NP-40, 1 mM DTT + Protease Inhibitors) for 2 h at 4°C. .. Bound proteins were washed five times in binding buffer, boiled in 2× SDS sample buffer and analysed by immunoblotting.

    Incubation:

    Article Title: A phospho-dependent mechanism involving NCoR and KMT2D controls a permissive chromatin state at Notch target genes
    Article Snippet: .. For Western blot purposes 500 μg of nuclear extract were incubated with streptavidin magnetic beads (Dynabeads M-280, Invitrogen 112.06) in a binding buffer containing 120 mM NaCl and 0.1% NP-40. .. After binding, beads were washed five times with streptavidin washing buffer containing 300 mM NaCl and two times with PBS.

    Article Title: Hepatoma-derived growth factor-related protein 2 promotes DNA repair by homologous recombination
    Article Snippet: .. Histone peptide pulldown Biotin-conjugated histone 3 (H3) peptides (Eurogentech) were immobilized on streptavidin magnetic beads (Life Technologies, Dynabeads M-280 Streptavidin) and incubated with nuclear extracts prepared as in the immunoprecipitation procedure but resolved in binding buffer (150 mM Hepes-KOH (pH7.6), 200 mM KCl, 1 mM MgCl2 , 1 mM EDTA, 10% Glycerol, 0.5% NP-40, 1 mM DTT + Protease Inhibitors) for 2 h at 4°C. .. Bound proteins were washed five times in binding buffer, boiled in 2× SDS sample buffer and analysed by immunoblotting.

    Western Blot:

    Article Title: A phospho-dependent mechanism involving NCoR and KMT2D controls a permissive chromatin state at Notch target genes
    Article Snippet: .. For Western blot purposes 500 μg of nuclear extract were incubated with streptavidin magnetic beads (Dynabeads M-280, Invitrogen 112.06) in a binding buffer containing 120 mM NaCl and 0.1% NP-40. .. After binding, beads were washed five times with streptavidin washing buffer containing 300 mM NaCl and two times with PBS.

    Binding Assay:

    Article Title: A phospho-dependent mechanism involving NCoR and KMT2D controls a permissive chromatin state at Notch target genes
    Article Snippet: .. For Western blot purposes 500 μg of nuclear extract were incubated with streptavidin magnetic beads (Dynabeads M-280, Invitrogen 112.06) in a binding buffer containing 120 mM NaCl and 0.1% NP-40. .. After binding, beads were washed five times with streptavidin washing buffer containing 300 mM NaCl and two times with PBS.

    Article Title: Hepatoma-derived growth factor-related protein 2 promotes DNA repair by homologous recombination
    Article Snippet: .. Histone peptide pulldown Biotin-conjugated histone 3 (H3) peptides (Eurogentech) were immobilized on streptavidin magnetic beads (Life Technologies, Dynabeads M-280 Streptavidin) and incubated with nuclear extracts prepared as in the immunoprecipitation procedure but resolved in binding buffer (150 mM Hepes-KOH (pH7.6), 200 mM KCl, 1 mM MgCl2 , 1 mM EDTA, 10% Glycerol, 0.5% NP-40, 1 mM DTT + Protease Inhibitors) for 2 h at 4°C. .. Bound proteins were washed five times in binding buffer, boiled in 2× SDS sample buffer and analysed by immunoblotting.

    Plasmid Preparation:

    Article Title: TRAP/SMCC/Mediator-Dependent Transcriptional Activation from DNA and Chromatin Templates by Orphan Nuclear Receptor Hepatocyte Nuclear Factor 4
    Article Snippet: .. 600 bp) from plasmid pA4xMLΔ53 ( , ), which contains the HNF-4 cognate sites and the core promoter elements, was filled in with biotinylated dATP by using the Klenow fragment of DNA Pol I, gel purified, and bound to M280-streptavidin Dynabeads (Dynal), as suggested by the manufacturer. ..

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    Thermo Fisher streptavidin coated magnetic beads
    Aβ22-41 binds to fibrinogen and fragment D. (A-B) Biotin-labeled Aβ42, Aβ1-16, Aβ15-25, and Aβ22-41 were incubated with fibrinogen (FBG) or fragment D (FD), and pulldown assays were carried out using <t>streptavidin-coated</t>
    Streptavidin Coated Magnetic Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 385 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin coated magnetic beads/product/Thermo Fisher
    Average 99 stars, based on 385 article reviews
    Price from $9.99 to $1999.99
    streptavidin coated magnetic beads - by Bioz Stars, 2020-05
    99/100 stars
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    Aβ22-41 binds to fibrinogen and fragment D. (A-B) Biotin-labeled Aβ42, Aβ1-16, Aβ15-25, and Aβ22-41 were incubated with fibrinogen (FBG) or fragment D (FD), and pulldown assays were carried out using streptavidin-coated

    Journal: Blood

    Article Title: Biochemical and structural analysis of the interaction between β-amyloid and fibrinogen

    doi: 10.1182/blood-2016-03-705228

    Figure Lengend Snippet: Aβ22-41 binds to fibrinogen and fragment D. (A-B) Biotin-labeled Aβ42, Aβ1-16, Aβ15-25, and Aβ22-41 were incubated with fibrinogen (FBG) or fragment D (FD), and pulldown assays were carried out using streptavidin-coated

    Article Snippet: Streptavidin-coated magnetic beads (Dynabeads M-280; Thermo-Fisher) were added for 30 minutes, washed, and eluted with nonreducing 1× lithium dodecyl sulfate sample buffer (Thermo Fisher Scientific).

    Techniques: Labeling, Incubation

    COUP-TFI Is Expressed in bmMSCs and Binds to the Ins2 Promoter (A) The DNA affinity precipitation assay was carried out with the nuclear extracts and biotinylated PCR products. The protein-DNA complexes were separated with streptavidin-labeled beads. The numbers 1 and 2 indicate nuclear extracts from MIN6 cells and from bmMSCs, respectively. (B and C) Western blotting (B) and semiquantitative PCR analysis (C) for COUP-TFI expression levels in MIN6 cells and bmMSCs. (D) MIN6 cells and bmMSCs were stained for COUP-TFI (left) and insulin (middle). Nuclei were stained with DAPI (right). Scale bars represent 50 μm.

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: Repression of COUP-TFI Improves Bone Marrow-Derived Mesenchymal Stem Cell Differentiation into Insulin-Producing Cells

    doi: 10.1016/j.omtn.2017.06.016

    Figure Lengend Snippet: COUP-TFI Is Expressed in bmMSCs and Binds to the Ins2 Promoter (A) The DNA affinity precipitation assay was carried out with the nuclear extracts and biotinylated PCR products. The protein-DNA complexes were separated with streptavidin-labeled beads. The numbers 1 and 2 indicate nuclear extracts from MIN6 cells and from bmMSCs, respectively. (B and C) Western blotting (B) and semiquantitative PCR analysis (C) for COUP-TFI expression levels in MIN6 cells and bmMSCs. (D) MIN6 cells and bmMSCs were stained for COUP-TFI (left) and insulin (middle). Nuclei were stained with DAPI (right). Scale bars represent 50 μm.

    Article Snippet: The nuclear extract (200 μg) was incubated at 4°C for 4 hr with biotinylated PCR products previously coupled to streptavidin-coated magnetic beads (Dynabeads M-280; Thermo Scientific).

    Techniques: Affinity Precipitation, Polymerase Chain Reaction, Labeling, Western Blot, Expressing, Staining