streptavidin coated m 280 dynabeads  (Thermo Fisher)


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    Name:
    Dynabeads M 280 Streptavidin
    Description:
    Dynabeads M 280 Streptavidin are the gold standard for the isolation and handling of biotinylated nucleic acids antibodies or other biotinylated ligands and targets The strong binding affinity of the streptavidin biotin interaction Kd 10 15 is used in a vast number of applications Benefits and features include • Direct and fast isolation of any biotinylated molecule• Low charged and neutral beads optimal for binding of DNA fragments proteins peptides and antibodies• Flexible protocols with gentle and efficient liquid phase reaction kinetics• Biomagnetic protocols are easily adapted to automated platforms• High batch to batch reproducibility securing consistent results in your applicationAbout Dynabeads M 280 StreptavidinThese uniform and superparamagnetic beads are 2 8 µm in diameter with a monolayer not a multilayer of recombinant streptavidin covalently coupled to the surface and further blocked with BSA The monolayer of streptavidin leaves the vast majority of the biotin binding sites sterically available for binding not only of free biotin but also for binding of biotinylated ligands targets They show rapid liquid phase reaction kinetics Their specific and defined surface allow for efficient capture separation and downstream handling The streptavidin monolayer ensures negligible leakage and the lack of excess adsorbed streptavidin ensures batch consistency and reproducibility of your results ApplicationsOver the past 15 years Dynabeads M 280 Streptavidin have been used and cited for a very wide variety of applications Key applications include preparing 2 5 kb samples for mate pair library sequencing using Illumina platforms preparing single stranded DNA templates isolation of RNA and DNA binding proteins immobilization of large DNA fragments purifying sequencing products and the specific capture of nucleic acids Dynabeads are used on more than 25 000 routine IVD instruments worldwide The product holds high standards with respect to reproducibility both within and between batches and automation ability and drives reliability for your results Binding capacityThe size of the molecule and the biotinylation procedure will affect the binding capacity The capacity also depends on steric availability and charge interaction between bead and molecule and between molecules There are two or three biotin binding sites available for each streptavidin molecule on the surface of the bead after immobilization One mg of Dynabeads M 280 Streptavidin typically binds • 650 900 pmoles free biotin• 200 pmol biotinylated peptides• 10 µg biotinylated IgG• 10 µg ds DNA• 200 pmol ss oligonucleotides
    Catalog Number:
    11205d
    Price:
    None
    Applications:
    Antibody Production and Labeling|Antibody Purification|ChIP-on-Chip|Chromatin Immunoprecipitation (ChIP)|DNA & RNA Purification & Analysis|DNA Extraction|Immunoprecipitation|Kits & Reagents for Sanger Sequencing|Phage Display|Protein Assays and Analysis|Protein Biology|Protein Complex Isolation & Analysis|Protein Purification|Protein Purification & Isolation|Protein-Protein Interactions|RNAi, Epigenetics & Non-Coding RNA Research|Sanger Sequencing|Sequencing Reaction Purification|Chromatin Biology|Sequencing
    Category:
    Beads Microspheres
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    Structured Review

    Thermo Fisher streptavidin coated m 280 dynabeads
    Dynabeads M 280 Streptavidin are the gold standard for the isolation and handling of biotinylated nucleic acids antibodies or other biotinylated ligands and targets The strong binding affinity of the streptavidin biotin interaction Kd 10 15 is used in a vast number of applications Benefits and features include • Direct and fast isolation of any biotinylated molecule• Low charged and neutral beads optimal for binding of DNA fragments proteins peptides and antibodies• Flexible protocols with gentle and efficient liquid phase reaction kinetics• Biomagnetic protocols are easily adapted to automated platforms• High batch to batch reproducibility securing consistent results in your applicationAbout Dynabeads M 280 StreptavidinThese uniform and superparamagnetic beads are 2 8 µm in diameter with a monolayer not a multilayer of recombinant streptavidin covalently coupled to the surface and further blocked with BSA The monolayer of streptavidin leaves the vast majority of the biotin binding sites sterically available for binding not only of free biotin but also for binding of biotinylated ligands targets They show rapid liquid phase reaction kinetics Their specific and defined surface allow for efficient capture separation and downstream handling The streptavidin monolayer ensures negligible leakage and the lack of excess adsorbed streptavidin ensures batch consistency and reproducibility of your results ApplicationsOver the past 15 years Dynabeads M 280 Streptavidin have been used and cited for a very wide variety of applications Key applications include preparing 2 5 kb samples for mate pair library sequencing using Illumina platforms preparing single stranded DNA templates isolation of RNA and DNA binding proteins immobilization of large DNA fragments purifying sequencing products and the specific capture of nucleic acids Dynabeads are used on more than 25 000 routine IVD instruments worldwide The product holds high standards with respect to reproducibility both within and between batches and automation ability and drives reliability for your results Binding capacityThe size of the molecule and the biotinylation procedure will affect the binding capacity The capacity also depends on steric availability and charge interaction between bead and molecule and between molecules There are two or three biotin binding sites available for each streptavidin molecule on the surface of the bead after immobilization One mg of Dynabeads M 280 Streptavidin typically binds • 650 900 pmoles free biotin• 200 pmol biotinylated peptides• 10 µg biotinylated IgG• 10 µg ds DNA• 200 pmol ss oligonucleotides
    https://www.bioz.com/result/streptavidin coated m 280 dynabeads/product/Thermo Fisher
    Average 90 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    streptavidin coated m 280 dynabeads - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Screening and Identifying a Novel ssDNA Aptamer against Alpha-fetoprotein Using CE-SELEX
    Article Snippet: Dynabeads M-280 Streptavidin was purchased from Invitrogen (Invitrogen Life Technologies, Carlsbad, CA, USA). .. Bovine serum albumin was purchased from Sigma-Aldrich (Saint Louis, MO, USA). pMDTM 19-T Vector Cloning Kit was purchased from Takara (TaKaRa Biomedicals, Shiga, Japan).

    Article Title: Development of PCR Assays for Species- and Type-Specific Identification of Pasteurella multocida Isolates
    Article Snippet: The incorporation of streptavidin-coated paramagnetic particles and a low-background cloning strategy ( , ) has exponentially increased the efficiency of the subtraction procedure and remains applicable to the employment of competitive reassociation of DNA fragments of any cell types to identify unique DNA sequences. .. This report details the replacement of Streptavidin Magnesphere Paramagnetic Particles (Promega, Sydney, Australia) with Dynabeads M-280 streptavidin (Dynal), allowing the addition of solid-phase driver fragments to ensure the enrichment of unique tester DNA sequences following magnetic separation.

    Amplification:

    Article Title: A tissue-specific landscape of sense/antisense transcription in the mouse intestine
    Article Snippet: 13 cycles of amplification led to a yield of more than 2 µg cDNA. .. Subsequently, SOLiD V2 fragment library protocol (Applied Biosystems) was applied and transcript ends were depleted by two rounds of Dynabeads M-280 streptavidin (Invitrogen) treatment.

    Article Title: Does BLM helicase unwind nucleosomal DNA?
    Article Snippet: Two types of DNA were amplified using 208-FT-208-R, and 208-FA-208-R to generate dT15 -208 and dA15 -208. .. The non-biotinylated strand of dT15 -208 and biotinylated strand of dA15 -208 are purified using Dynabeads M-280 Streptavidin (Invitrogen, Dynal AS, Oslo, Norway) as described previously ( ).

    Filtration:

    Article Title: Nuclear Receptors TR2 and TR4 Recruit Multiple Epigenetic Transcriptional Corepressors That Associate Specifically with the Embryonic ?-Type Globin Promoters in Differentiated Adult Erythroid Cells ▿
    Article Snippet: After pooling fractions 20 to 38 from the preparative gel filtration, the KCl and NP-40 concentrations were adjusted to 150 mM and 0.3%, respectively. .. Diluted, pooled fractions were then divided into 50-ml Falcon tubes, to which Dynabeads M-280–streptavidin (Invitrogen), preblocked as described previously , was added for overnight incubation at 4°C on a rotating wheel.

    Synthesized:

    Article Title: RNA Splicing at Human Immunodeficiency Virus Type 1 3? Splice Site A2 Is Regulated by Binding of hnRNP A/B Proteins to an Exonic Splicing Silencer Element
    Article Snippet: Substrate RNAs for bead immobilization were synthesized by in vitro transcription using T7 RNA polymerase (Ambion, Austin, Tex.) and biotin-14 CTP (Life Technologies/Gibco/BRL) with a ratio of modified nucleotide to standard nucleotide of 1:2. .. RNAs were noncovalently linked to Dynabeads M-280–streptavidin (6.7 × 108 beads/ml) (Dynal, Lake Success, N.Y.) as suggested by the manufacturer.

    Article Title: RNAs Are Packaged into Human Cytomegalovirus Virions in Proportion to Their Intracellular Concentration
    Article Snippet: Sense-strand UL21.5 RNA was synthesized in vitro with the RiboMax large scale RNA production system (Promega). .. Biotinylated UL21.5 RNA (≈10 μg) was bound to 75 μl of Dynabeads M-280 streptavidin (Dynal Biotech) according to the manufacturer's instructions.

    Quantitative RT-PCR:

    Article Title: Mir-338-3p Inhibits Malignant Biological Behaviors of Glioma Cells by Targeting MACC1 Gene
    Article Snippet: The samples were incubated with Dynabeads M-280 Streptavidin (Invitrogen, CA) following the manufacturer’s protocol. .. The bound RNAs were obtained and analyzed by qRT-PCR.

    Incubation:

    Article Title: Nuclear Receptors TR2 and TR4 Recruit Multiple Epigenetic Transcriptional Corepressors That Associate Specifically with the Embryonic ?-Type Globin Promoters in Differentiated Adult Erythroid Cells ▿
    Article Snippet: .. Diluted, pooled fractions were then divided into 50-ml Falcon tubes, to which Dynabeads M-280–streptavidin (Invitrogen), preblocked as described previously , was added for overnight incubation at 4°C on a rotating wheel. ..

    Article Title: Transcription of the Schizosaccharomyces pombe U2 gene in vivo and in vitro is directed by two essential promoter elements
    Article Snippet: .. Biotinylated spUSE DNA or control DNA (1 µg) was incubated with 1.0 mg Dynabeads M-280 streptavidin (Dynal, Inc., Oslo, Norway) in 1× binding and washing buffer (10 mM Tris–HCl pH 7.5, 1 mM EDTA and 1 M NaCl) for 30 min at room temperature. .. After washing three times with 1× binding and washing buffer, the beads were re-suspended in 100 µl Buffer A (50 mM HEPES pH 7.5, 90 mM potassium glutamate, 10 mM magnesium acetate, 5 mM EGTA, 10% glycerol, 0.75% PEG-3350).

    Article Title: RNA Splicing at Human Immunodeficiency Virus Type 1 3? Splice Site A2 Is Regulated by Binding of hnRNP A/B Proteins to an Exonic Splicing Silencer Element
    Article Snippet: RNAs were noncovalently linked to Dynabeads M-280–streptavidin (6.7 × 108 beads/ml) (Dynal, Lake Success, N.Y.) as suggested by the manufacturer. .. Two micrograms of RNA was incubated with 40 μl of Dynabeads in 1 M NaCl–10 mM Tris HCl (pH 7.5)–1 mM EDTA at room temperature for 15 min with gentle agitation.

    Article Title: Mir-338-3p Inhibits Malignant Biological Behaviors of Glioma Cells by Targeting MACC1 Gene
    Article Snippet: .. The samples were incubated with Dynabeads M-280 Streptavidin (Invitrogen, CA) following the manufacturer’s protocol. .. Biotinylated miR-338-3p was incubated with beads for 10 min, and then treated with washing buffer.

    Article Title: RNAs Are Packaged into Human Cytomegalovirus Virions in Proportion to Their Intracellular Concentration
    Article Snippet: Virus extract was prepared by pelleting cell-free virus through 20% sorbitol and resuspending in lysis buffer (10 mM Tris-HCl, pH 7.8, 50 mM NaCl, 2 mM MgCl2 , 1 mM dithiothreitol, 0.1% Nonidet P-40) containing EDTA-free complete protease inhibitor cocktail (Roche) and incubated for 1 h at 4°C prior to use. .. Biotinylated UL21.5 RNA (≈10 μg) was bound to 75 μl of Dynabeads M-280 streptavidin (Dynal Biotech) according to the manufacturer's instructions.

    Article Title: Identification of strong intron enhancer in the heparanase gene: effect of functional rs4693608 variant on HPSE enhancer activity in hematological and solid malignancies
    Article Snippet: The pull-down assay was performed using Dynabeads M-280 Streptavidin (Invitrogene by Thermo Fisher Scientific, Norway). .. An aliquot of 20 µl of beads was washed in PBS-T (0.1% Triton X-100) and incubated with 500 ng of each biotinylated DNA probe overnight at 4 °C.

    Article Title: Ctk1 promotes dissociation of basal transcription factors from elongating RNA polymerase II
    Article Snippet: Dynabeads M-280 Streptavidin (Dynal) were concentrated with a magnetic particle concentrator (MPC) (Dynal) and washed twice in Buffer T (10 mM Tris (pH 7.5), 1 mM EDTA, 1 M NaCl). .. Dynabeads were then incubated with 8.6 ng biotinylated template per μg of bead in Buffer T for 30 min at room temperature with constant agitation.

    Article Title: Does BLM helicase unwind nucleosomal DNA?
    Article Snippet: For the 18 bp replication fork mimic, the biotin-labeled 50-R and 27lead, and the 50-F2 and 27lag are incubated pair-wise at 95°C for 5 min, followed by a 15 min incubation at 65°C and an hour incubation at 37°C; then the two annealed pairs were mixed and incubated at 25°C for 12–18 h. For the 35 bp forked-DNA, the biotin-labeled 50-R and 50-F1 were incubated at 95°C for 5 min, followed by a 15 min incubation at 65°C and an hour incubation at 37°C. .. The non-biotinylated strand of dT15 -208 and biotinylated strand of dA15 -208 are purified using Dynabeads M-280 Streptavidin (Invitrogen, Dynal AS, Oslo, Norway) as described previously ( ).

    Formalin-fixed Paraffin-Embedded:

    Article Title: Detection of DNA fusion junctions for BCR-ABL translocations by Anchored ChromPET
    Article Snippet: .. Reagents Reagents used were APex Heat-Labile Alkaline Phosphatase (Epicentre, Madison, WI, USA; AP49010), Biotin-16-UTP (Roche, Indianapolis, IN, USA; 11388908910), DNAZol reagent (Invitrogen, Carlsbad, CA, USA; 10503-027), Dynabeads M-280 streptavidin (Invitorgen; 112-05D), End-It DNA End Repair Kit (Epicentre; ER0720), human Cot-1 DNA (Invitrogen; 15279-011), MAXIscript Kit (Ambion, Austin, TX, USA; AM1312), MinElute Reaction Cleanup Kit (Qiagen, Valencia, CA, USA; 28204), pCR4-TOPO-TA vector (Invitrogen; K4575-01), QIAquick Gel Extraction Kit (Qiagen; 28704), QIAquick PCR Purification Kit (Qiagen; 28104), QuickExtract FFPE DNA Extraction Kit (Epicentre; QEF81805), QuickExtract FFPE RNA Extraction Kit (Epicentre; QFR82805), Quick Ligation Kit (NEB, Ipswich, MA, USA; M2200S), SuperScript III Reverse Transcriptase (Invitrogen; 18080-093), TaKaRa Ex Taq DNA Polymerase (Takara, Otsu, Shiga, Japan; TAK RR001A), Taq DNA Polymerase (Roche; 11146165001), TRIzol (Invitrogen; 15596-026), and TURBO DNase (Ambion; AM2238). .. Cell lines K562 cells (CCL-243) and KU812 cells (CRL-2099) were purchased from ATCC and cultured according to ATCC instructions.

    Expressing:

    Article Title: A tissue-specific landscape of sense/antisense transcription in the mouse intestine
    Article Snippet: Subsequently, SOLiD V2 fragment library protocol (Applied Biosystems) was applied and transcript ends were depleted by two rounds of Dynabeads M-280 streptavidin (Invitrogen) treatment. .. The full datasets have been submitted to a public data repository (Gene Expression Omnibus, http://www.ncbi.nlm.nih.gov/geo accession number: GSE21746 ).

    Modification:

    Article Title: RNA Splicing at Human Immunodeficiency Virus Type 1 3? Splice Site A2 Is Regulated by Binding of hnRNP A/B Proteins to an Exonic Splicing Silencer Element
    Article Snippet: Substrate RNAs for bead immobilization were synthesized by in vitro transcription using T7 RNA polymerase (Ambion, Austin, Tex.) and biotin-14 CTP (Life Technologies/Gibco/BRL) with a ratio of modified nucleotide to standard nucleotide of 1:2. .. RNAs were noncovalently linked to Dynabeads M-280–streptavidin (6.7 × 108 beads/ml) (Dynal, Lake Success, N.Y.) as suggested by the manufacturer.

    Article Title: Gαi Signaling Promotes Marginal Zone B Cell Development by Enabling Transitional B Cell ADAM10 Expression
    Article Snippet: Splenic B cells were isolated by negative depletion using biotinylated antibodies to CD4, CD8, CD11b, and CD11c and Dynabeads M-280 Streptavidin (Thermo Fisher Scientific). .. Bone marrow cells were cultured in complete Iscove’s Modified Dulbecco’s Medium (GIBCO) in the presence of IL-7 (20 ng/ml) for 4 days to enrich for IgM+ immature B cells.

    Hybridization:

    Article Title: Development of PCR Assays for Species- and Type-Specific Identification of Pasteurella multocida Isolates
    Article Snippet: The original subtractive hybridization method described was designed to isolate and clone differentially expressed mRNA sequences ( ). .. This report details the replacement of Streptavidin Magnesphere Paramagnetic Particles (Promega, Sydney, Australia) with Dynabeads M-280 streptavidin (Dynal), allowing the addition of solid-phase driver fragments to ensure the enrichment of unique tester DNA sequences following magnetic separation.

    Transfection:

    Article Title: Mir-338-3p Inhibits Malignant Biological Behaviors of Glioma Cells by Targeting MACC1 Gene
    Article Snippet: Pull-down assay MiR-338-3p, miR-338-3p-Mut, and miR-338-3p-NC were biotinylated to be bio-miR-338-3p-wt, bio-miR-338-3p-mut, and bio-NC, respectively, by GenePharma Company (Shanghai, China), and transfected into glioma cells. .. The samples were incubated with Dynabeads M-280 Streptavidin (Invitrogen, CA) following the manufacturer’s protocol.

    Chromatography:

    Article Title: Transcription of the Schizosaccharomyces pombe U2 gene in vivo and in vitro is directed by two essential promoter elements
    Article Snippet: Biotinylated spUSE DNA or control DNA (1 µg) was incubated with 1.0 mg Dynabeads M-280 streptavidin (Dynal, Inc., Oslo, Norway) in 1× binding and washing buffer (10 mM Tris–HCl pH 7.5, 1 mM EDTA and 1 M NaCl) for 30 min at room temperature. .. Fraction 45 (320 µl; obtained by gel-filtration chromatography as described above) was incubated with 40 µl of DNA-coupled Dynabeads in 1× Buffer A for 30 min at room temperature.

    Ligation:

    Article Title: A tissue-specific landscape of sense/antisense transcription in the mouse intestine
    Article Snippet: Subsequently, SOLiD V2 fragment library protocol (Applied Biosystems) was applied and transcript ends were depleted by two rounds of Dynabeads M-280 streptavidin (Invitrogen) treatment. .. First type of libraries (cDNA fragmented) was sequenced on a SOLiD V2 and V2.5 (replicates) sequencing by ligation sequencer following manufacturer's instructions, second type of libraries (RNA fragmented) on a SOLiD V4.

    Article Title: Detection of DNA fusion junctions for BCR-ABL translocations by Anchored ChromPET
    Article Snippet: .. Reagents Reagents used were APex Heat-Labile Alkaline Phosphatase (Epicentre, Madison, WI, USA; AP49010), Biotin-16-UTP (Roche, Indianapolis, IN, USA; 11388908910), DNAZol reagent (Invitrogen, Carlsbad, CA, USA; 10503-027), Dynabeads M-280 streptavidin (Invitorgen; 112-05D), End-It DNA End Repair Kit (Epicentre; ER0720), human Cot-1 DNA (Invitrogen; 15279-011), MAXIscript Kit (Ambion, Austin, TX, USA; AM1312), MinElute Reaction Cleanup Kit (Qiagen, Valencia, CA, USA; 28204), pCR4-TOPO-TA vector (Invitrogen; K4575-01), QIAquick Gel Extraction Kit (Qiagen; 28704), QIAquick PCR Purification Kit (Qiagen; 28104), QuickExtract FFPE DNA Extraction Kit (Epicentre; QEF81805), QuickExtract FFPE RNA Extraction Kit (Epicentre; QFR82805), Quick Ligation Kit (NEB, Ipswich, MA, USA; M2200S), SuperScript III Reverse Transcriptase (Invitrogen; 18080-093), TaKaRa Ex Taq DNA Polymerase (Takara, Otsu, Shiga, Japan; TAK RR001A), Taq DNA Polymerase (Roche; 11146165001), TRIzol (Invitrogen; 15596-026), and TURBO DNase (Ambion; AM2238). .. Cell lines K562 cells (CCL-243) and KU812 cells (CRL-2099) were purchased from ATCC and cultured according to ATCC instructions.

    Protease Inhibitor:

    Article Title: RNAs Are Packaged into Human Cytomegalovirus Virions in Proportion to Their Intracellular Concentration
    Article Snippet: Virus extract was prepared by pelleting cell-free virus through 20% sorbitol and resuspending in lysis buffer (10 mM Tris-HCl, pH 7.8, 50 mM NaCl, 2 mM MgCl2 , 1 mM dithiothreitol, 0.1% Nonidet P-40) containing EDTA-free complete protease inhibitor cocktail (Roche) and incubated for 1 h at 4°C prior to use. .. Biotinylated UL21.5 RNA (≈10 μg) was bound to 75 μl of Dynabeads M-280 streptavidin (Dynal Biotech) according to the manufacturer's instructions.

    Article Title: Identification of strong intron enhancer in the heparanase gene: effect of functional rs4693608 variant on HPSE enhancer activity in hematological and solid malignancies
    Article Snippet: The pull-down assay was performed using Dynabeads M-280 Streptavidin (Invitrogene by Thermo Fisher Scientific, Norway). .. The beads were washed in PBS-T and then in pull-down wash buffer containing 20% glycerol, 0.1 M KCl, 0.2 mM EDTA, 20 mM Hepes-KOH pH 7.9, 1 mM DTT, 0.1% Triton X-100, 0.1× Halt Protease inhibitor Cocktail (Thermo Scientifec, Rockford, IL, USA), 0.1 mM PMSF.

    Cell Culture:

    Article Title: Gαi Signaling Promotes Marginal Zone B Cell Development by Enabling Transitional B Cell ADAM10 Expression
    Article Snippet: Paragraph title: Cells and Cell Culture ... Splenic B cells were isolated by negative depletion using biotinylated antibodies to CD4, CD8, CD11b, and CD11c and Dynabeads M-280 Streptavidin (Thermo Fisher Scientific).

    Sequencing:

    Article Title: A tissue-specific landscape of sense/antisense transcription in the mouse intestine
    Article Snippet: Subsequently, SOLiD V2 fragment library protocol (Applied Biosystems) was applied and transcript ends were depleted by two rounds of Dynabeads M-280 streptavidin (Invitrogen) treatment. .. First type of libraries (cDNA fragmented) was sequenced on a SOLiD V2 and V2.5 (replicates) sequencing by ligation sequencer following manufacturer's instructions, second type of libraries (RNA fragmented) on a SOLiD V4.

    Article Title: Structural and Functional Analyses of DNA-Sensing and Immune Activation by Human cGAS
    Article Snippet: DNA-binding experiment The cGAS wild type and mutant proteins were mixed with biotinylated ISD (Interferon stimulatory DNA, Sense strand sequence 5′-TACAGATCTACTAGTGATCTATGACTGATCTGTACATGATCTACA-3′ ) at room temperature for 1 hour. .. They were then mixed with Dynabeads M-280 Streptavidin (Life Technologies) in buffer (50 mM Tris-HCl (pH 7.5) containing 100 mM NaCl, 10% glycerol, and 0.5% NP-40) for 1 hour.

    Article Title: Does BLM helicase unwind nucleosomal DNA?
    Article Snippet: For the nucleosomal DNA, a 208 bp nucleosome positioning sequence from 5S rDNA of sea urchin was used for the template ( ). .. The non-biotinylated strand of dT15 -208 and biotinylated strand of dA15 -208 are purified using Dynabeads M-280 Streptavidin (Invitrogen, Dynal AS, Oslo, Norway) as described previously ( ).

    Affinity Purification:

    Article Title: Transcription of the Schizosaccharomyces pombe U2 gene in vivo and in vitro is directed by two essential promoter elements
    Article Snippet: Paragraph title: DNA affinity purification of spUSE binding proteins ... Biotinylated spUSE DNA or control DNA (1 µg) was incubated with 1.0 mg Dynabeads M-280 streptavidin (Dynal, Inc., Oslo, Norway) in 1× binding and washing buffer (10 mM Tris–HCl pH 7.5, 1 mM EDTA and 1 M NaCl) for 30 min at room temperature.

    Article Title: RNAs Are Packaged into Human Cytomegalovirus Virions in Proportion to Their Intracellular Concentration
    Article Snippet: Paragraph title: Affinity purification. ... Biotinylated UL21.5 RNA (≈10 μg) was bound to 75 μl of Dynabeads M-280 streptavidin (Dynal Biotech) according to the manufacturer's instructions.

    Binding Assay:

    Article Title: Nuclear Receptors TR2 and TR4 Recruit Multiple Epigenetic Transcriptional Corepressors That Associate Specifically with the Embryonic ?-Type Globin Promoters in Differentiated Adult Erythroid Cells ▿
    Article Snippet: Paragraph title: Binding to streptavidin beads. ... Diluted, pooled fractions were then divided into 50-ml Falcon tubes, to which Dynabeads M-280–streptavidin (Invitrogen), preblocked as described previously , was added for overnight incubation at 4°C on a rotating wheel.

    Article Title: Transcription of the Schizosaccharomyces pombe U2 gene in vivo and in vitro is directed by two essential promoter elements
    Article Snippet: .. Biotinylated spUSE DNA or control DNA (1 µg) was incubated with 1.0 mg Dynabeads M-280 streptavidin (Dynal, Inc., Oslo, Norway) in 1× binding and washing buffer (10 mM Tris–HCl pH 7.5, 1 mM EDTA and 1 M NaCl) for 30 min at room temperature. .. After washing three times with 1× binding and washing buffer, the beads were re-suspended in 100 µl Buffer A (50 mM HEPES pH 7.5, 90 mM potassium glutamate, 10 mM magnesium acetate, 5 mM EGTA, 10% glycerol, 0.75% PEG-3350).

    Article Title: Mitotic phosphorylation of histone H3 threonine 80
    Article Snippet: .. Peptide binding assay Twenty microlitre of Streptavidin-coupled Dynabeads® (Invitrogen, http://products.invitrogen.com/ivgn/product/11205D ) were washed in binding buffer (50 mM Tris pH7.5, 100 mM NaCl, 0.05% NP40). .. Beads were re-suspended in 195 µl of binding buffer.

    DNA Extraction:

    Article Title: Detection of DNA fusion junctions for BCR-ABL translocations by Anchored ChromPET
    Article Snippet: .. Reagents Reagents used were APex Heat-Labile Alkaline Phosphatase (Epicentre, Madison, WI, USA; AP49010), Biotin-16-UTP (Roche, Indianapolis, IN, USA; 11388908910), DNAZol reagent (Invitrogen, Carlsbad, CA, USA; 10503-027), Dynabeads M-280 streptavidin (Invitorgen; 112-05D), End-It DNA End Repair Kit (Epicentre; ER0720), human Cot-1 DNA (Invitrogen; 15279-011), MAXIscript Kit (Ambion, Austin, TX, USA; AM1312), MinElute Reaction Cleanup Kit (Qiagen, Valencia, CA, USA; 28204), pCR4-TOPO-TA vector (Invitrogen; K4575-01), QIAquick Gel Extraction Kit (Qiagen; 28704), QIAquick PCR Purification Kit (Qiagen; 28104), QuickExtract FFPE DNA Extraction Kit (Epicentre; QEF81805), QuickExtract FFPE RNA Extraction Kit (Epicentre; QFR82805), Quick Ligation Kit (NEB, Ipswich, MA, USA; M2200S), SuperScript III Reverse Transcriptase (Invitrogen; 18080-093), TaKaRa Ex Taq DNA Polymerase (Takara, Otsu, Shiga, Japan; TAK RR001A), Taq DNA Polymerase (Roche; 11146165001), TRIzol (Invitrogen; 15596-026), and TURBO DNase (Ambion; AM2238). .. Cell lines K562 cells (CCL-243) and KU812 cells (CRL-2099) were purchased from ATCC and cultured according to ATCC instructions.

    RNA Sequencing Assay:

    Article Title: A tissue-specific landscape of sense/antisense transcription in the mouse intestine
    Article Snippet: Paragraph title: RNA-Seq ... Subsequently, SOLiD V2 fragment library protocol (Applied Biosystems) was applied and transcript ends were depleted by two rounds of Dynabeads M-280 streptavidin (Invitrogen) treatment.

    Pull Down Assay:

    Article Title: Mir-338-3p Inhibits Malignant Biological Behaviors of Glioma Cells by Targeting MACC1 Gene
    Article Snippet: Paragraph title: Pull-down assay ... The samples were incubated with Dynabeads M-280 Streptavidin (Invitrogen, CA) following the manufacturer’s protocol.

    Article Title: Identification of strong intron enhancer in the heparanase gene: effect of functional rs4693608 variant on HPSE enhancer activity in hematological and solid malignancies
    Article Snippet: .. The pull-down assay was performed using Dynabeads M-280 Streptavidin (Invitrogene by Thermo Fisher Scientific, Norway). .. An aliquot of 20 µl of beads was washed in PBS-T (0.1% Triton X-100) and incubated with 500 ng of each biotinylated DNA probe overnight at 4 °C.

    Mutagenesis:

    Article Title: Structural and Functional Analyses of DNA-Sensing and Immune Activation by Human cGAS
    Article Snippet: DNA-binding experiment The cGAS wild type and mutant proteins were mixed with biotinylated ISD (Interferon stimulatory DNA, Sense strand sequence 5′-TACAGATCTACTAGTGATCTATGACTGATCTGTACATGATCTACA-3′ ) at room temperature for 1 hour. .. They were then mixed with Dynabeads M-280 Streptavidin (Life Technologies) in buffer (50 mM Tris-HCl (pH 7.5) containing 100 mM NaCl, 10% glycerol, and 0.5% NP-40) for 1 hour.

    Isolation:

    Article Title: Gαi Signaling Promotes Marginal Zone B Cell Development by Enabling Transitional B Cell ADAM10 Expression
    Article Snippet: .. Splenic B cells were isolated by negative depletion using biotinylated antibodies to CD4, CD8, CD11b, and CD11c and Dynabeads M-280 Streptavidin (Thermo Fisher Scientific). ..

    Labeling:

    Article Title: RNAs Are Packaged into Human Cytomegalovirus Virions in Proportion to Their Intracellular Concentration
    Article Snippet: UL21.5 RNA (20 μg) was linked to biotin with the 5′ EndTag nucleic acid labeling system (Vector Laboratories) and biotin maleimide (Vector Laboratories) following the manufacturer's instructions. .. Biotinylated UL21.5 RNA (≈10 μg) was bound to 75 μl of Dynabeads M-280 streptavidin (Dynal Biotech) according to the manufacturer's instructions.

    Purification:

    Article Title: Screening and Identifying a Novel ssDNA Aptamer against Alpha-fetoprotein Using CE-SELEX
    Article Snippet: Chemicals, ssDNA and cell lines The purified AFP protein (100 μg/ml) was purchased from Fitzgerald (Fitzgerald, MA, USA). .. Dynabeads M-280 Streptavidin was purchased from Invitrogen (Invitrogen Life Technologies, Carlsbad, CA, USA).

    Article Title: Detection of DNA fusion junctions for BCR-ABL translocations by Anchored ChromPET
    Article Snippet: .. Reagents Reagents used were APex Heat-Labile Alkaline Phosphatase (Epicentre, Madison, WI, USA; AP49010), Biotin-16-UTP (Roche, Indianapolis, IN, USA; 11388908910), DNAZol reagent (Invitrogen, Carlsbad, CA, USA; 10503-027), Dynabeads M-280 streptavidin (Invitorgen; 112-05D), End-It DNA End Repair Kit (Epicentre; ER0720), human Cot-1 DNA (Invitrogen; 15279-011), MAXIscript Kit (Ambion, Austin, TX, USA; AM1312), MinElute Reaction Cleanup Kit (Qiagen, Valencia, CA, USA; 28204), pCR4-TOPO-TA vector (Invitrogen; K4575-01), QIAquick Gel Extraction Kit (Qiagen; 28704), QIAquick PCR Purification Kit (Qiagen; 28104), QuickExtract FFPE DNA Extraction Kit (Epicentre; QEF81805), QuickExtract FFPE RNA Extraction Kit (Epicentre; QFR82805), Quick Ligation Kit (NEB, Ipswich, MA, USA; M2200S), SuperScript III Reverse Transcriptase (Invitrogen; 18080-093), TaKaRa Ex Taq DNA Polymerase (Takara, Otsu, Shiga, Japan; TAK RR001A), Taq DNA Polymerase (Roche; 11146165001), TRIzol (Invitrogen; 15596-026), and TURBO DNase (Ambion; AM2238). .. Cell lines K562 cells (CCL-243) and KU812 cells (CRL-2099) were purchased from ATCC and cultured according to ATCC instructions.

    Article Title: RNAs Are Packaged into Human Cytomegalovirus Virions in Proportion to Their Intracellular Concentration
    Article Snippet: Unincorporated nucleotides were removed through several precipitation steps followed by purification with a Sephadex G-50 column (Roche). .. Biotinylated UL21.5 RNA (≈10 μg) was bound to 75 μl of Dynabeads M-280 streptavidin (Dynal Biotech) according to the manufacturer's instructions.

    Article Title: Does BLM helicase unwind nucleosomal DNA?
    Article Snippet: .. The non-biotinylated strand of dT15 -208 and biotinylated strand of dA15 -208 are purified using Dynabeads M-280 Streptavidin (Invitrogen, Dynal AS, Oslo, Norway) as described previously ( ). .. The two ssDNAs were mixed in an equal proportion and were incubated at 95°C for 5 min, followed by a gradual (1°C/min) decrease to 37°C to generate dT15 forked-DNA.

    Polymerase Chain Reaction:

    Article Title: Transcription of the Schizosaccharomyces pombe U2 gene in vivo and in vitro is directed by two essential promoter elements
    Article Snippet: The control biotinylated DNA was obtained the same way except that insertless pSP1 vector was used as the PCR template. .. Biotinylated spUSE DNA or control DNA (1 µg) was incubated with 1.0 mg Dynabeads M-280 streptavidin (Dynal, Inc., Oslo, Norway) in 1× binding and washing buffer (10 mM Tris–HCl pH 7.5, 1 mM EDTA and 1 M NaCl) for 30 min at room temperature.

    Article Title: A tissue-specific landscape of sense/antisense transcription in the mouse intestine
    Article Snippet: For second strand synthesis and amplification, a 5'-biotinylated version of PCR primer II was employed. .. Subsequently, SOLiD V2 fragment library protocol (Applied Biosystems) was applied and transcript ends were depleted by two rounds of Dynabeads M-280 streptavidin (Invitrogen) treatment.

    Article Title: Screening and Identifying a Novel ssDNA Aptamer against Alpha-fetoprotein Using CE-SELEX
    Article Snippet: Taq PCR MasterMix and 50 bp DNA Ladder were purchased from Tiangen (Tiangen Biotech Co., Ltd., Beijing, China). .. Dynabeads M-280 Streptavidin was purchased from Invitrogen (Invitrogen Life Technologies, Carlsbad, CA, USA).

    Article Title: Detection of DNA fusion junctions for BCR-ABL translocations by Anchored ChromPET
    Article Snippet: .. Reagents Reagents used were APex Heat-Labile Alkaline Phosphatase (Epicentre, Madison, WI, USA; AP49010), Biotin-16-UTP (Roche, Indianapolis, IN, USA; 11388908910), DNAZol reagent (Invitrogen, Carlsbad, CA, USA; 10503-027), Dynabeads M-280 streptavidin (Invitorgen; 112-05D), End-It DNA End Repair Kit (Epicentre; ER0720), human Cot-1 DNA (Invitrogen; 15279-011), MAXIscript Kit (Ambion, Austin, TX, USA; AM1312), MinElute Reaction Cleanup Kit (Qiagen, Valencia, CA, USA; 28204), pCR4-TOPO-TA vector (Invitrogen; K4575-01), QIAquick Gel Extraction Kit (Qiagen; 28704), QIAquick PCR Purification Kit (Qiagen; 28104), QuickExtract FFPE DNA Extraction Kit (Epicentre; QEF81805), QuickExtract FFPE RNA Extraction Kit (Epicentre; QFR82805), Quick Ligation Kit (NEB, Ipswich, MA, USA; M2200S), SuperScript III Reverse Transcriptase (Invitrogen; 18080-093), TaKaRa Ex Taq DNA Polymerase (Takara, Otsu, Shiga, Japan; TAK RR001A), Taq DNA Polymerase (Roche; 11146165001), TRIzol (Invitrogen; 15596-026), and TURBO DNase (Ambion; AM2238). .. Cell lines K562 cells (CCL-243) and KU812 cells (CRL-2099) were purchased from ATCC and cultured according to ATCC instructions.

    Article Title: Ctk1 promotes dissociation of basal transcription factors from elongating RNA polymerase II
    Article Snippet: Immobilized template assay Biotinylated templates were prepared by PCR as described previously, with pSH515 as a template ( ). .. Dynabeads M-280 Streptavidin (Dynal) were concentrated with a magnetic particle concentrator (MPC) (Dynal) and washed twice in Buffer T (10 mM Tris (pH 7.5), 1 mM EDTA, 1 M NaCl).

    Article Title: Development of PCR Assays for Species- and Type-Specific Identification of Pasteurella multocida Isolates
    Article Snippet: This report details the replacement of Streptavidin Magnesphere Paramagnetic Particles (Promega, Sydney, Australia) with Dynabeads M-280 streptavidin (Dynal), allowing the addition of solid-phase driver fragments to ensure the enrichment of unique tester DNA sequences following magnetic separation. .. Oligonucleotide primers designed from cloned subtracted fragments have contributed to the development of PCR-based assays for species- and type-specific identification of P. multocida and of P. multocida type B, the causal agent of HS.

    Blocking Assay:

    Article Title: Ctk1 promotes dissociation of basal transcription factors from elongating RNA polymerase II
    Article Snippet: Dynabeads M-280 Streptavidin (Dynal) were concentrated with a magnetic particle concentrator (MPC) (Dynal) and washed twice in Buffer T (10 mM Tris (pH 7.5), 1 mM EDTA, 1 M NaCl). .. Immobilized templates were washed once in Buffer T and blocked in blocking buffer (1 ml/mg beads) for 15 min at room temperature.

    Gel Extraction:

    Article Title: Detection of DNA fusion junctions for BCR-ABL translocations by Anchored ChromPET
    Article Snippet: .. Reagents Reagents used were APex Heat-Labile Alkaline Phosphatase (Epicentre, Madison, WI, USA; AP49010), Biotin-16-UTP (Roche, Indianapolis, IN, USA; 11388908910), DNAZol reagent (Invitrogen, Carlsbad, CA, USA; 10503-027), Dynabeads M-280 streptavidin (Invitorgen; 112-05D), End-It DNA End Repair Kit (Epicentre; ER0720), human Cot-1 DNA (Invitrogen; 15279-011), MAXIscript Kit (Ambion, Austin, TX, USA; AM1312), MinElute Reaction Cleanup Kit (Qiagen, Valencia, CA, USA; 28204), pCR4-TOPO-TA vector (Invitrogen; K4575-01), QIAquick Gel Extraction Kit (Qiagen; 28704), QIAquick PCR Purification Kit (Qiagen; 28104), QuickExtract FFPE DNA Extraction Kit (Epicentre; QEF81805), QuickExtract FFPE RNA Extraction Kit (Epicentre; QFR82805), Quick Ligation Kit (NEB, Ipswich, MA, USA; M2200S), SuperScript III Reverse Transcriptase (Invitrogen; 18080-093), TaKaRa Ex Taq DNA Polymerase (Takara, Otsu, Shiga, Japan; TAK RR001A), Taq DNA Polymerase (Roche; 11146165001), TRIzol (Invitrogen; 15596-026), and TURBO DNase (Ambion; AM2238). .. Cell lines K562 cells (CCL-243) and KU812 cells (CRL-2099) were purchased from ATCC and cultured according to ATCC instructions.

    IA:

    Article Title: Does BLM helicase unwind nucleosomal DNA?
    Article Snippet: All oligonucleotides used for the preparation of DNA substrates were purchased from Integrated DNA Technologies, Inc (Coralville, IA). .. The non-biotinylated strand of dT15 -208 and biotinylated strand of dA15 -208 are purified using Dynabeads M-280 Streptavidin (Invitrogen, Dynal AS, Oslo, Norway) as described previously ( ).

    SDS Page:

    Article Title: Nuclear Receptors TR2 and TR4 Recruit Multiple Epigenetic Transcriptional Corepressors That Associate Specifically with the Embryonic ?-Type Globin Promoters in Differentiated Adult Erythroid Cells ▿
    Article Snippet: Diluted, pooled fractions were then divided into 50-ml Falcon tubes, to which Dynabeads M-280–streptavidin (Invitrogen), preblocked as described previously , was added for overnight incubation at 4°C on a rotating wheel. .. Bound proteins were eluted with 1× Laemmli sample buffer (50 μl of sample buffer per 20 μl of beads), and then resolved by SDS-PAGE.

    Plasmid Preparation:

    Article Title: Transcription of the Schizosaccharomyces pombe U2 gene in vivo and in vitro is directed by two essential promoter elements
    Article Snippet: The control biotinylated DNA was obtained the same way except that insertless pSP1 vector was used as the PCR template. .. Biotinylated spUSE DNA or control DNA (1 µg) was incubated with 1.0 mg Dynabeads M-280 streptavidin (Dynal, Inc., Oslo, Norway) in 1× binding and washing buffer (10 mM Tris–HCl pH 7.5, 1 mM EDTA and 1 M NaCl) for 30 min at room temperature.

    Article Title: Screening and Identifying a Novel ssDNA Aptamer against Alpha-fetoprotein Using CE-SELEX
    Article Snippet: Dynabeads M-280 Streptavidin was purchased from Invitrogen (Invitrogen Life Technologies, Carlsbad, CA, USA). .. Bovine serum albumin was purchased from Sigma-Aldrich (Saint Louis, MO, USA). pMDTM 19-T Vector Cloning Kit was purchased from Takara (TaKaRa Biomedicals, Shiga, Japan).

    Article Title: Detection of DNA fusion junctions for BCR-ABL translocations by Anchored ChromPET
    Article Snippet: .. Reagents Reagents used were APex Heat-Labile Alkaline Phosphatase (Epicentre, Madison, WI, USA; AP49010), Biotin-16-UTP (Roche, Indianapolis, IN, USA; 11388908910), DNAZol reagent (Invitrogen, Carlsbad, CA, USA; 10503-027), Dynabeads M-280 streptavidin (Invitorgen; 112-05D), End-It DNA End Repair Kit (Epicentre; ER0720), human Cot-1 DNA (Invitrogen; 15279-011), MAXIscript Kit (Ambion, Austin, TX, USA; AM1312), MinElute Reaction Cleanup Kit (Qiagen, Valencia, CA, USA; 28204), pCR4-TOPO-TA vector (Invitrogen; K4575-01), QIAquick Gel Extraction Kit (Qiagen; 28704), QIAquick PCR Purification Kit (Qiagen; 28104), QuickExtract FFPE DNA Extraction Kit (Epicentre; QEF81805), QuickExtract FFPE RNA Extraction Kit (Epicentre; QFR82805), Quick Ligation Kit (NEB, Ipswich, MA, USA; M2200S), SuperScript III Reverse Transcriptase (Invitrogen; 18080-093), TaKaRa Ex Taq DNA Polymerase (Takara, Otsu, Shiga, Japan; TAK RR001A), Taq DNA Polymerase (Roche; 11146165001), TRIzol (Invitrogen; 15596-026), and TURBO DNase (Ambion; AM2238). .. Cell lines K562 cells (CCL-243) and KU812 cells (CRL-2099) were purchased from ATCC and cultured according to ATCC instructions.

    RNA Extraction:

    Article Title: Detection of DNA fusion junctions for BCR-ABL translocations by Anchored ChromPET
    Article Snippet: .. Reagents Reagents used were APex Heat-Labile Alkaline Phosphatase (Epicentre, Madison, WI, USA; AP49010), Biotin-16-UTP (Roche, Indianapolis, IN, USA; 11388908910), DNAZol reagent (Invitrogen, Carlsbad, CA, USA; 10503-027), Dynabeads M-280 streptavidin (Invitorgen; 112-05D), End-It DNA End Repair Kit (Epicentre; ER0720), human Cot-1 DNA (Invitrogen; 15279-011), MAXIscript Kit (Ambion, Austin, TX, USA; AM1312), MinElute Reaction Cleanup Kit (Qiagen, Valencia, CA, USA; 28204), pCR4-TOPO-TA vector (Invitrogen; K4575-01), QIAquick Gel Extraction Kit (Qiagen; 28704), QIAquick PCR Purification Kit (Qiagen; 28104), QuickExtract FFPE DNA Extraction Kit (Epicentre; QEF81805), QuickExtract FFPE RNA Extraction Kit (Epicentre; QFR82805), Quick Ligation Kit (NEB, Ipswich, MA, USA; M2200S), SuperScript III Reverse Transcriptase (Invitrogen; 18080-093), TaKaRa Ex Taq DNA Polymerase (Takara, Otsu, Shiga, Japan; TAK RR001A), Taq DNA Polymerase (Roche; 11146165001), TRIzol (Invitrogen; 15596-026), and TURBO DNase (Ambion; AM2238). .. Cell lines K562 cells (CCL-243) and KU812 cells (CRL-2099) were purchased from ATCC and cultured according to ATCC instructions.

    Recombinant:

    Article Title: Mitotic phosphorylation of histone H3 threonine 80
    Article Snippet: Peptide binding assay Twenty microlitre of Streptavidin-coupled Dynabeads® (Invitrogen, http://products.invitrogen.com/ivgn/product/11205D ) were washed in binding buffer (50 mM Tris pH7.5, 100 mM NaCl, 0.05% NP40). .. Ten microliters of 0.45 µg/µl recombinant histone octamers , were added and allowed to bind on a nutator for 2–3 h at 4 °C.

    In Vitro:

    Article Title: RNA Splicing at Human Immunodeficiency Virus Type 1 3? Splice Site A2 Is Regulated by Binding of hnRNP A/B Proteins to an Exonic Splicing Silencer Element
    Article Snippet: Substrate RNAs for bead immobilization were synthesized by in vitro transcription using T7 RNA polymerase (Ambion, Austin, Tex.) and biotin-14 CTP (Life Technologies/Gibco/BRL) with a ratio of modified nucleotide to standard nucleotide of 1:2. .. RNAs were noncovalently linked to Dynabeads M-280–streptavidin (6.7 × 108 beads/ml) (Dynal, Lake Success, N.Y.) as suggested by the manufacturer.

    Article Title: RNAs Are Packaged into Human Cytomegalovirus Virions in Proportion to Their Intracellular Concentration
    Article Snippet: Sense-strand UL21.5 RNA was synthesized in vitro with the RiboMax large scale RNA production system (Promega). .. Biotinylated UL21.5 RNA (≈10 μg) was bound to 75 μl of Dynabeads M-280 streptavidin (Dynal Biotech) according to the manufacturer's instructions.

    Lysis:

    Article Title: RNAs Are Packaged into Human Cytomegalovirus Virions in Proportion to Their Intracellular Concentration
    Article Snippet: Virus extract was prepared by pelleting cell-free virus through 20% sorbitol and resuspending in lysis buffer (10 mM Tris-HCl, pH 7.8, 50 mM NaCl, 2 mM MgCl2 , 1 mM dithiothreitol, 0.1% Nonidet P-40) containing EDTA-free complete protease inhibitor cocktail (Roche) and incubated for 1 h at 4°C prior to use. .. Biotinylated UL21.5 RNA (≈10 μg) was bound to 75 μl of Dynabeads M-280 streptavidin (Dynal Biotech) according to the manufacturer's instructions.

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    Thermo Fisher streptavidin coated m 280 dynabeads
    Streptavidin Coated M 280 Dynabeads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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