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Roche lysis buffer b
Lysis Buffer B, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lysis buffer b/product/Roche
Average 92 stars, based on 53 article reviews
Price from $9.99 to $1999.99
lysis buffer b - by Bioz Stars, 2020-07
92/100 stars

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Centrifugation:

Article Title: Multiple and Variable NHEJ-Like Genes Are Involved in Resistance to DNA Damage in Streptomyces ambofaciens
Article Snippet: .. Cells were harvested by centrifugation and the pellet was resuspended in 40 mL lysis buffer B (50 mM Tris-HCl pH 8, 0.25 M NaCl, 0.2 mg/mL lysozyme) supplemented with Complete Antiprotease (Roche) and incubated 1 h at 20°C. .. The mixture was then centrifuged and the pellet was treated with DnaseI, as described for KuA purification.

Article Title: Phospho-tyrosine dependent protein–protein interaction network
Article Snippet: .. For each binding reaction, 50 ml bacterial culture was harvested by centrifugation at 2,000× g for 20 min, resuspended in 0.5 ml lysis buffer B [50 mM Hepes (pH 7.4); 150 mM NaCl; 1 mM EDTA; 5% glycerol; 1 mM DTT; 1 mg/ml lysozyme; 0.5% Brij 58; protease inhibitor (Roche, 11836170001)] and lysed by sonication. .. Fractions of the lysates with comparable amount of soluble protein were mixed for immobilization in a final volume of 0.5 ml lysis buffer B with 50 μl of 50% glutathione agarose slurry and incubated for 1 h. The agarose beads were washed twice with lysis buffer B and resuspended as a 50% slurry in lysis buffer H [50 mM Hepes (pH 7.4); 150 mM NaCl; 1 mM EDTA; 10% glycerol; 1% Triton X-100; 1% Phosphatase Inhibitor cocktail 2 (Sigma-Aldrich, P5726), Protease Inhibitor (Roche, 11836170001)].

Protease Inhibitor:

Article Title: Decreased Tissue COX5B Expression and Mitochondrial Dysfunction during Sepsis-Induced Kidney Injury in Rats
Article Snippet: .. The final volume of each sample was adjusted to 100 μ L with lysis buffer B [7 M urea; 2 M thiourea; 4% CHAPS; Roche complete protease inhibitor cocktail; Pefablock SC protease inhibitor] and samples were supplemented with 2% Servalyte and 1% DTT. .. Finally, 350 μ L of rehydration buffer [6 M urea; 2 M thiourea; 2% CHAPS; 1% DTT; 1 v/v% Servalyte 3–10 Iso-Dalt for 2D, Roche complete protease inhibitor cocktail; Pefablock SC protease inhibitor] was added to each sample.

Article Title: Interaction between RING1 (R1) and the Ubiquitin-like (UBL) Domains Is Critical for the Regulation of Parkin Activity *
Article Snippet: .. Cells were lysed with Lysis buffer B (2% SDS, 150 m m NaCl, and 10 m m Tris, pH 8.0) with the protease inhibitor mixture (Roche Applied Science). .. Lysis buffer A and lysis buffer B were mixed at a 10:1 ratio.

Article Title: Glycolysis promotes caspase-3 activation in lipid rafts in T cells
Article Snippet: .. bVAD active caspase precipitation Cells were washed once with PBS containing 1% bovine serum albumin (PBS/1% BSA) and once with PBS, and then incubated on ice for at least 20 min with Lysis Buffer B (0.2% Nonidet P-40,150 mM NaCl, 20 mM Tris-HCl (pH 7.4), 10% glycerol, 2 mM sodium orthovanadate, and Complete Protease Inhibitor (Roche Diagnostics)) supplemented with 20 μM biotin-VAD-fmk (bVAD; MP Biomedicals). ..

Article Title: Phospho-tyrosine dependent protein–protein interaction network
Article Snippet: .. For each binding reaction, 50 ml bacterial culture was harvested by centrifugation at 2,000× g for 20 min, resuspended in 0.5 ml lysis buffer B [50 mM Hepes (pH 7.4); 150 mM NaCl; 1 mM EDTA; 5% glycerol; 1 mM DTT; 1 mg/ml lysozyme; 0.5% Brij 58; protease inhibitor (Roche, 11836170001)] and lysed by sonication. .. Fractions of the lysates with comparable amount of soluble protein were mixed for immobilization in a final volume of 0.5 ml lysis buffer B with 50 μl of 50% glutathione agarose slurry and incubated for 1 h. The agarose beads were washed twice with lysis buffer B and resuspended as a 50% slurry in lysis buffer H [50 mM Hepes (pH 7.4); 150 mM NaCl; 1 mM EDTA; 10% glycerol; 1% Triton X-100; 1% Phosphatase Inhibitor cocktail 2 (Sigma-Aldrich, P5726), Protease Inhibitor (Roche, 11836170001)].

Incubation:

Article Title: Nuclear F-actin and myosins drive relocalization of heterochromatic breaks
Article Snippet: .. Pellets were incubated in lysis buffer B (50 mM Tris, 150 mM NaCl, 2 mM EDTA, 10% glycerol, 0.2% Nonidet P-40, protease inhibitors (Complete, Roche), 1 mM PMSF, and 25 mM NEM) for 30 min at 4 °C. .. Lysates were centrifuged and the supernatant was incubated with FLAG-M2 agarose beads (Sigma).

Article Title: Multiple and Variable NHEJ-Like Genes Are Involved in Resistance to DNA Damage in Streptomyces ambofaciens
Article Snippet: .. Cells were harvested by centrifugation and the pellet was resuspended in 40 mL lysis buffer B (50 mM Tris-HCl pH 8, 0.25 M NaCl, 0.2 mg/mL lysozyme) supplemented with Complete Antiprotease (Roche) and incubated 1 h at 20°C. .. The mixture was then centrifuged and the pellet was treated with DnaseI, as described for KuA purification.

Article Title: Glycolysis promotes caspase-3 activation in lipid rafts in T cells
Article Snippet: .. bVAD active caspase precipitation Cells were washed once with PBS containing 1% bovine serum albumin (PBS/1% BSA) and once with PBS, and then incubated on ice for at least 20 min with Lysis Buffer B (0.2% Nonidet P-40,150 mM NaCl, 20 mM Tris-HCl (pH 7.4), 10% glycerol, 2 mM sodium orthovanadate, and Complete Protease Inhibitor (Roche Diagnostics)) supplemented with 20 μM biotin-VAD-fmk (bVAD; MP Biomedicals). ..

Immunoprecipitation:

Article Title: Defects of Vps15 in skeletal muscles lead to autophagic vacuolar myopathy and lysosomal disease
Article Snippet: .. Protein extraction, immunoblotting and immunoprecipitation For immunoblot analysis, cells were washed twice with cold phosphate-buffered saline (PBS), scraped off of the culture dish in lysis buffer B containing 50 mM Tris (pH 8.0), 1% NP-40, 120 mM NaCl, 20 mM NaF, 1× protease inhibitors (Roche), 1× PhosphoStop Inhibitors (Roche). .. Protein extract from muscle tissue was prepared in 20 mM Tris-HCl (pH 8.0), 5% glycerol, 138 mM NaCl, 2.7 mM KCl, 1% NP-40, 20 mM NaF, 5 mM EDTA, 1× protease inhibitors (Roche), 1× PhosphoStop Inhibitors (Roche).

Protein Extraction:

Article Title: Defects of Vps15 in skeletal muscles lead to autophagic vacuolar myopathy and lysosomal disease
Article Snippet: .. Protein extraction, immunoblotting and immunoprecipitation For immunoblot analysis, cells were washed twice with cold phosphate-buffered saline (PBS), scraped off of the culture dish in lysis buffer B containing 50 mM Tris (pH 8.0), 1% NP-40, 120 mM NaCl, 20 mM NaF, 1× protease inhibitors (Roche), 1× PhosphoStop Inhibitors (Roche). .. Protein extract from muscle tissue was prepared in 20 mM Tris-HCl (pH 8.0), 5% glycerol, 138 mM NaCl, 2.7 mM KCl, 1% NP-40, 20 mM NaF, 5 mM EDTA, 1× protease inhibitors (Roche), 1× PhosphoStop Inhibitors (Roche).

Lysis:

Article Title: Reconstitution and quantification of dynamic microtubule end tracking in vitro using TIRF microscopy.
Article Snippet: .. Several microtubule-associated proteins localize in living cells selectively to an extended region at the growing microtubule plus ends. ..

Article Title: Decreased Tissue COX5B Expression and Mitochondrial Dysfunction during Sepsis-Induced Kidney Injury in Rats
Article Snippet: .. The final volume of each sample was adjusted to 100 μ L with lysis buffer B [7 M urea; 2 M thiourea; 4% CHAPS; Roche complete protease inhibitor cocktail; Pefablock SC protease inhibitor] and samples were supplemented with 2% Servalyte and 1% DTT. .. Finally, 350 μ L of rehydration buffer [6 M urea; 2 M thiourea; 2% CHAPS; 1% DTT; 1 v/v% Servalyte 3–10 Iso-Dalt for 2D, Roche complete protease inhibitor cocktail; Pefablock SC protease inhibitor] was added to each sample.

Article Title: Interaction between RING1 (R1) and the Ubiquitin-like (UBL) Domains Is Critical for the Regulation of Parkin Activity *
Article Snippet: .. Cells were lysed with Lysis buffer B (2% SDS, 150 m m NaCl, and 10 m m Tris, pH 8.0) with the protease inhibitor mixture (Roche Applied Science). .. Lysis buffer A and lysis buffer B were mixed at a 10:1 ratio.

Article Title: Nuclear F-actin and myosins drive relocalization of heterochromatic breaks
Article Snippet: .. Pellets were incubated in lysis buffer B (50 mM Tris, 150 mM NaCl, 2 mM EDTA, 10% glycerol, 0.2% Nonidet P-40, protease inhibitors (Complete, Roche), 1 mM PMSF, and 25 mM NEM) for 30 min at 4 °C. .. Lysates were centrifuged and the supernatant was incubated with FLAG-M2 agarose beads (Sigma).

Article Title: Multiple and Variable NHEJ-Like Genes Are Involved in Resistance to DNA Damage in Streptomyces ambofaciens
Article Snippet: .. Cells were harvested by centrifugation and the pellet was resuspended in 40 mL lysis buffer B (50 mM Tris-HCl pH 8, 0.25 M NaCl, 0.2 mg/mL lysozyme) supplemented with Complete Antiprotease (Roche) and incubated 1 h at 20°C. .. The mixture was then centrifuged and the pellet was treated with DnaseI, as described for KuA purification.

Article Title: Glycolysis promotes caspase-3 activation in lipid rafts in T cells
Article Snippet: .. bVAD active caspase precipitation Cells were washed once with PBS containing 1% bovine serum albumin (PBS/1% BSA) and once with PBS, and then incubated on ice for at least 20 min with Lysis Buffer B (0.2% Nonidet P-40,150 mM NaCl, 20 mM Tris-HCl (pH 7.4), 10% glycerol, 2 mM sodium orthovanadate, and Complete Protease Inhibitor (Roche Diagnostics)) supplemented with 20 μM biotin-VAD-fmk (bVAD; MP Biomedicals). ..

Article Title: Defects of Vps15 in skeletal muscles lead to autophagic vacuolar myopathy and lysosomal disease
Article Snippet: .. Protein extraction, immunoblotting and immunoprecipitation For immunoblot analysis, cells were washed twice with cold phosphate-buffered saline (PBS), scraped off of the culture dish in lysis buffer B containing 50 mM Tris (pH 8.0), 1% NP-40, 120 mM NaCl, 20 mM NaF, 1× protease inhibitors (Roche), 1× PhosphoStop Inhibitors (Roche). .. Protein extract from muscle tissue was prepared in 20 mM Tris-HCl (pH 8.0), 5% glycerol, 138 mM NaCl, 2.7 mM KCl, 1% NP-40, 20 mM NaF, 5 mM EDTA, 1× protease inhibitors (Roche), 1× PhosphoStop Inhibitors (Roche).

Article Title: Phospho-tyrosine dependent protein–protein interaction network
Article Snippet: .. For each binding reaction, 50 ml bacterial culture was harvested by centrifugation at 2,000× g for 20 min, resuspended in 0.5 ml lysis buffer B [50 mM Hepes (pH 7.4); 150 mM NaCl; 1 mM EDTA; 5% glycerol; 1 mM DTT; 1 mg/ml lysozyme; 0.5% Brij 58; protease inhibitor (Roche, 11836170001)] and lysed by sonication. .. Fractions of the lysates with comparable amount of soluble protein were mixed for immobilization in a final volume of 0.5 ml lysis buffer B with 50 μl of 50% glutathione agarose slurry and incubated for 1 h. The agarose beads were washed twice with lysis buffer B and resuspended as a 50% slurry in lysis buffer H [50 mM Hepes (pH 7.4); 150 mM NaCl; 1 mM EDTA; 10% glycerol; 1% Triton X-100; 1% Phosphatase Inhibitor cocktail 2 (Sigma-Aldrich, P5726), Protease Inhibitor (Roche, 11836170001)].

Sonication:

Article Title: Phospho-tyrosine dependent protein–protein interaction network
Article Snippet: .. For each binding reaction, 50 ml bacterial culture was harvested by centrifugation at 2,000× g for 20 min, resuspended in 0.5 ml lysis buffer B [50 mM Hepes (pH 7.4); 150 mM NaCl; 1 mM EDTA; 5% glycerol; 1 mM DTT; 1 mg/ml lysozyme; 0.5% Brij 58; protease inhibitor (Roche, 11836170001)] and lysed by sonication. .. Fractions of the lysates with comparable amount of soluble protein were mixed for immobilization in a final volume of 0.5 ml lysis buffer B with 50 μl of 50% glutathione agarose slurry and incubated for 1 h. The agarose beads were washed twice with lysis buffer B and resuspended as a 50% slurry in lysis buffer H [50 mM Hepes (pH 7.4); 150 mM NaCl; 1 mM EDTA; 10% glycerol; 1% Triton X-100; 1% Phosphatase Inhibitor cocktail 2 (Sigma-Aldrich, P5726), Protease Inhibitor (Roche, 11836170001)].

Binding Assay:

Article Title: Phospho-tyrosine dependent protein–protein interaction network
Article Snippet: .. For each binding reaction, 50 ml bacterial culture was harvested by centrifugation at 2,000× g for 20 min, resuspended in 0.5 ml lysis buffer B [50 mM Hepes (pH 7.4); 150 mM NaCl; 1 mM EDTA; 5% glycerol; 1 mM DTT; 1 mg/ml lysozyme; 0.5% Brij 58; protease inhibitor (Roche, 11836170001)] and lysed by sonication. .. Fractions of the lysates with comparable amount of soluble protein were mixed for immobilization in a final volume of 0.5 ml lysis buffer B with 50 μl of 50% glutathione agarose slurry and incubated for 1 h. The agarose beads were washed twice with lysis buffer B and resuspended as a 50% slurry in lysis buffer H [50 mM Hepes (pH 7.4); 150 mM NaCl; 1 mM EDTA; 10% glycerol; 1% Triton X-100; 1% Phosphatase Inhibitor cocktail 2 (Sigma-Aldrich, P5726), Protease Inhibitor (Roche, 11836170001)].

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    Roche cathepsin b cell lysis buffer kit
    <t>Cathepsin</t> B inhibition by E64 does not lead to α-synuclein accumulation
    Cathepsin B Cell Lysis Buffer Kit, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cathepsin b cell lysis buffer kit/product/Roche
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cathepsin b cell lysis buffer kit - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    92
    Roche lysis buffer b
    <t>Cathepsin</t> B inhibition by E64 does not lead to α-synuclein accumulation
    Lysis Buffer B, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lysis buffer b/product/Roche
    Average 92 stars, based on 53 article reviews
    Price from $9.99 to $1999.99
    lysis buffer b - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    Image Search Results


    Cathepsin B inhibition by E64 does not lead to α-synuclein accumulation

    Journal: Journal of neurochemistry

    Article Title: Overexpression of an inactive mutant cathepsin D increases endogenous alpha-synuclein and cathepsin B activity in SH-SY5Y cells*

    doi: 10.1111/jnc.12497

    Figure Lengend Snippet: Cathepsin B inhibition by E64 does not lead to α-synuclein accumulation

    Article Snippet: Briefly, cells were collected by scraping and centrifugation (1500 x g for 5 minutes at 4°C) and then lysed using cathepsin B cell lysis buffer (kit) with added protease (Roche) and phosphatase (Sigma) inhibitors followed by 30 minutes incubation on ice.

    Techniques: Inhibition