Structured Review

Millipore lysine
Lysine, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lysine/product/Millipore
Average 86 stars, based on 5 article reviews
Price from $9.99 to $1999.99
lysine - by Bioz Stars, 2020-09
86/100 stars

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Related Articles

Electron Microscopy:

Article Title: A Carbohydrate Moiety of Secreted Stage-Specific Glycoprotein 4 Participates in Host Cell Invasion by Trypanosoma cruzi Extracellular Amastigotes
Article Snippet: .. For scanning electron microscopy (SEM), EAs adhered to Vero cells or to coverslips covered with 0.1% poly-L-lysine (Sigma-Aldrich) were fixed with 4% paraformaldehyde, washed with 0.1 M cacodylate solution, post-fixed with osmium tetroxide, treated with tannic acid, dehydrated with ethanol, dried in a critical point dryer (Bal-Tec AG, Balzers, Liechtenstein), and sputtered with gold. ..

Labeling:

Article Title: Dose-Response of Superparamagnetic Iron Oxide Labeling on Mesenchymal Stem Cells Chondrogenic Differentiation: A Multi-Scale In Vitro Study
Article Snippet: .. Labeling of MSCs in monolayers with superparamagnetic iron oxide particles (SPIO) MSC labeling was initiated by the addition of medium that contained superparamagnetic iron oxide (SPIO) (Endorem, Guerbet S.A., Paris, France) and Poly-L-Lysine mixture (375 ng/mL, PLL Mw: 70,000–150,000, Sigma) and incubation was at 37°C/5% CO2 for 24 hours. .. Different concentrations of iron were tested for the labeling: without PLL, and PLL associated with 12.5 µg/mL, 25 µg/mL, 50 µg/mL, 100 µg/ml, 200 µg/ml, 400 µg/ml, 800 µg/ml and 1600 µg/ml.

Incubation:

Article Title: Dose-Response of Superparamagnetic Iron Oxide Labeling on Mesenchymal Stem Cells Chondrogenic Differentiation: A Multi-Scale In Vitro Study
Article Snippet: .. Labeling of MSCs in monolayers with superparamagnetic iron oxide particles (SPIO) MSC labeling was initiated by the addition of medium that contained superparamagnetic iron oxide (SPIO) (Endorem, Guerbet S.A., Paris, France) and Poly-L-Lysine mixture (375 ng/mL, PLL Mw: 70,000–150,000, Sigma) and incubation was at 37°C/5% CO2 for 24 hours. .. Different concentrations of iron were tested for the labeling: without PLL, and PLL associated with 12.5 µg/mL, 25 µg/mL, 50 µg/mL, 100 µg/ml, 200 µg/ml, 400 µg/ml, 800 µg/ml and 1600 µg/ml.

other:

Article Title: Production of Interleukin-8 by Human Neutrophils Stimulated with Trichomonas vaginalis
Article Snippet: Leupeptin (acetyl-Leu-Leu-Arg-al), TLCK ( N α- p -tosyl- l -lysine chloromethyl ketone), TPCK ( N -tosyl- l -phenylethyl chloromethyl ketone), EDTA, colchicine, cytochalasin D, cycloheximide, PIPES [piperazine- N , N ′-bis(2-ethanesulfonic acid)], and Histopaque 1077 were purchased from Sigma (St. Louis, Mo.).

Article Title: Importance of Protein-tyrosine Phosphatase-? Catalytic Domains for Interactions with SHP-2 and Interleukin-1-induced Matrix Metalloproteinase-3 Expression *
Article Snippet: Fibronectin, poly- l -lysine, BSA, puromycin, doxycycline, and mouse monoclonal antibodies to vinculin, phosphotyrosine (clone PY-20), and β-actin were from Sigma.

Activity Assay:

Article Title: Imaging effector functions of human cytotoxic CD4+ T cells specific for Plasmodium falciparum circumsporozoite protein ✰
Article Snippet: .. The activity was determined by adding 30 μl of sample supernatant to 170 μl of 20 mM Tris-HCl, pH 7.5, containing 1 mM EDTA, 220 μM 5,5′-dithio-bis-(2-nitrobenzoic acid), and 200 μM N-benzyloxycarbonyl-L-lysine thiobenzyl ester (BLT) (all from Sigma) as a substrate solution. ..

Modification:

Article Title: Enhancement of Peripheral Nerve Regrowth by the Purine Nucleoside Analog and Cell Cycle Inhibitor, Roscovitine
Article Snippet: .. After the final spin, the cells were resuspended in L15 and passed through a 70 mm mesh (VWR International Co.) and then placed in 500 ml L15 enriched with 1:100 dilution of N2 supplement (Invitrogen) and 0.1% BSA (Sigma) and placed into a culture medium of Dulbecco’s Modified Eagle Medium/F12 (DMEM/F12; Invitrogen) + 1:100 dilution N-2 supplement, 0.5–0.8% BSA and 0.2 ng/ml NGF (Cedarlane Labs) plus Penicillin 50 U/ml, Streptomycin 50 U/ml (Invitrogen) and plated on poly-L-lysine (Sigma-Aldrich) and 10 μg/ml mouse laminin (Invitrogen)-coated cover slips. ..

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  • 99
    Millipore anti mono ubiquitinated histone h2a lysine 119
    Dramatically decreased mono-ubiquitination of histone <t>H2A</t> <t>lysine</t> 119 (H2AK119u1) and phosphorylation of enhancer of zeste homolog 2 (Ezh2) methyltransferase in parthenogenetic blastocysts. Immunostaining was used to reveal that levels of total Ezh2 methyltransferase
    Anti Mono Ubiquitinated Histone H2a Lysine 119, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mono ubiquitinated histone h2a lysine 119/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti mono ubiquitinated histone h2a lysine 119 - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    91
    Millipore lysine 9
    Comparison of global histone modification patterns of KSHV genomes in BrK.219 and PEL cells. Global patterns of histone H3 trimethylated at lysine 4 (H3K4-me3), <t>lysine</t> 9 (H3K9-me3), or lysine 27 (H3K27-me3) or acetylated at lysine 9 and/or lysine 14 (H3K27-me3
    Lysine 9, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lysine 9/product/Millipore
    Average 91 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    lysine 9 - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    91
    Millipore lysine 4
    Chromatin immunoprecipitation using antibodies specific for methylated, unmodified and acetylated histones . Representative results of quantified DNA derived from unstimulated (basal) and 5-aza-CdR- or TSA-stimulated DU145 and MCF-7 cells immunoprecipitated by antibodies specific for methylated histones (left diagrams) as well as for unmodified and acetylated histones (right diagrams). Examples of PTEN in DU145 cells (A, B), CD44 in MCF-7 cells (C, D), GLIPR1 in DU145 cells (E, F) and Cyclin D2 in DU145 cells (G, H) are shown. All values obtained were normalized and referred to 100% of the input DNA. IgG, negative control; H3K9, Lysine 9 of histone H3; H3K4, <t>Lysine</t> 4 of histone H3; H4K20, Lysine 20 of histone H4; H2A, histone H2A; H2B, histone H2B; me, mono-methylated; me2, dimethylated; me3, trimethylated; Ac, acetylated.
    Lysine 4, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lysine 4/product/Millipore
    Average 91 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    lysine 4 - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    Image Search Results


    Dramatically decreased mono-ubiquitination of histone H2A lysine 119 (H2AK119u1) and phosphorylation of enhancer of zeste homolog 2 (Ezh2) methyltransferase in parthenogenetic blastocysts. Immunostaining was used to reveal that levels of total Ezh2 methyltransferase

    Journal: Stem Cells and Development

    Article Title: Epigenetic Disruptions of Histone Signatures for the Trophectoderm and Inner Cell Mass in Mouse Parthenogenetic Embryos

    doi: 10.1089/scd.2014.0310

    Figure Lengend Snippet: Dramatically decreased mono-ubiquitination of histone H2A lysine 119 (H2AK119u1) and phosphorylation of enhancer of zeste homolog 2 (Ezh2) methyltransferase in parthenogenetic blastocysts. Immunostaining was used to reveal that levels of total Ezh2 methyltransferase

    Article Snippet: After blocking, embryos were incubated at 4°C overnight with the following primary antibodies diluted in blocking solution: anti-acetylated histone H3 lysine 9 (rabbit polyclonal, 1:200 dilution; Abcam), anti-CARM1 (both mouse monoclonal and rabbit polyclonal, 1:200 dilution; Abcam), anti-Cdx2 (mouse monoclonal, 1:100 dilution; BioGenex), anti-dimethylated histone H3 arginine 26 (rabbit polyclonal, 1:200 dilution; Abcam), anti-Ezh2 (rabbit polyclonal, 1:200 dilution; Millipore), anti-mono-ubiquitinated histone H2A lysine 119 (mouse monoclonal, 1:200 dilution; Millipore), anti-phosphorylated Akt1 (phospho-S473) (rabbit polyclonal, 1:100 dilution; Abcam), anti-phosphorylated Ezh2 (phospho-S21) (rabbit polyclonal, 1:100 dilution; Abcam), anti-TCF7L2 (rabbit polyclonal, 1:100 dilution; Abcam), anti-trimethylated histone H3 lysine 4 (rabbit polyclonal, 1:200 dilution; Abcam), anti-trimethylated histone H3 lysine 9 (rabbit polyclonal, 1:200 dilution; Millipore), or anti-trimethylated histone H3 lysine 27 (rabbit polyclonal, 1:200 dilution; Millipore).

    Techniques: Immunostaining

    Comparison of global histone modification patterns of KSHV genomes in BrK.219 and PEL cells. Global patterns of histone H3 trimethylated at lysine 4 (H3K4-me3), lysine 9 (H3K9-me3), or lysine 27 (H3K27-me3) or acetylated at lysine 9 and/or lysine 14 (H3K27-me3

    Journal: Journal of Virology

    Article Title: Activation of the B Cell Antigen Receptor Triggers Reactivation of Latent Kaposi's Sarcoma-Associated Herpesvirus in B Cells

    doi: 10.1128/JVI.00506-13

    Figure Lengend Snippet: Comparison of global histone modification patterns of KSHV genomes in BrK.219 and PEL cells. Global patterns of histone H3 trimethylated at lysine 4 (H3K4-me3), lysine 9 (H3K9-me3), or lysine 27 (H3K27-me3) or acetylated at lysine 9 and/or lysine 14 (H3K27-me3

    Article Snippet: Briefly, chromatin was prepared from KSHV-infected BrK.219 cells and subjected to immunoprecipitation with antibodies specific for histone H3 trimethylated at lysine 4, lysine 9, or lysine 27 or acetylated at lysine 9 and/or lysine 14 (H3K4-me3, H3K9-me3, H3K27-me3, and H3K9/14-ac [Upstate/Millipore]) or with a matched isotype control antibody (IgG).

    Techniques: Modification

    Chromatin immunoprecipitation using antibodies specific for methylated, unmodified and acetylated histones . Representative results of quantified DNA derived from unstimulated (basal) and 5-aza-CdR- or TSA-stimulated DU145 and MCF-7 cells immunoprecipitated by antibodies specific for methylated histones (left diagrams) as well as for unmodified and acetylated histones (right diagrams). Examples of PTEN in DU145 cells (A, B), CD44 in MCF-7 cells (C, D), GLIPR1 in DU145 cells (E, F) and Cyclin D2 in DU145 cells (G, H) are shown. All values obtained were normalized and referred to 100% of the input DNA. IgG, negative control; H3K9, Lysine 9 of histone H3; H3K4, Lysine 4 of histone H3; H4K20, Lysine 20 of histone H4; H2A, histone H2A; H2B, histone H2B; me, mono-methylated; me2, dimethylated; me3, trimethylated; Ac, acetylated.

    Journal: BMC Cancer

    Article Title: Promoter- and cell-specific epigenetic regulation of CD44, Cyclin D2, GLIPR1 and PTEN by Methyl-CpG binding proteins and histone modifications

    doi: 10.1186/1471-2407-10-297

    Figure Lengend Snippet: Chromatin immunoprecipitation using antibodies specific for methylated, unmodified and acetylated histones . Representative results of quantified DNA derived from unstimulated (basal) and 5-aza-CdR- or TSA-stimulated DU145 and MCF-7 cells immunoprecipitated by antibodies specific for methylated histones (left diagrams) as well as for unmodified and acetylated histones (right diagrams). Examples of PTEN in DU145 cells (A, B), CD44 in MCF-7 cells (C, D), GLIPR1 in DU145 cells (E, F) and Cyclin D2 in DU145 cells (G, H) are shown. All values obtained were normalized and referred to 100% of the input DNA. IgG, negative control; H3K9, Lysine 9 of histone H3; H3K4, Lysine 4 of histone H3; H4K20, Lysine 20 of histone H4; H2A, histone H2A; H2B, histone H2B; me, mono-methylated; me2, dimethylated; me3, trimethylated; Ac, acetylated.

    Article Snippet: After blocking, the membrane was probed with a 1:500 or 1:1000 dilution of antibodies which are directed against the following human proteins: MBD1, MeCP2 (Abcam, Cambridge, UK), MBD2, monomethylated lysine 9 of histone H3 (H3K9me), dimethylated lysine 9 of histone H3 (H3K9me2), trimethylated lysine 9 of histone H3 (H3K9me3), monomethylated lysine 4 of histone H3 (H3K4me), dimethylated lysine 4 of histone H3 (H3K4me2), trimethylated lysine 4 of histone H3 (H3K4me3), monomethylated lysine 20 of histone H4 (H4K20me), dimethylated lysine 20 of histone H4 (H4K20me2), trimethylated lysine 20 of histone H4 (H4K20me3) (Millipore, Schwalbach, Germany).

    Techniques: Chromatin Immunoprecipitation, Methylation, Derivative Assay, Immunoprecipitation, Negative Control