luria bertani media  (Millipore)


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    Structured Review

    Millipore luria bertani media
    Luria Bertani Media, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luria bertani media/product/Millipore
    Average 97 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    luria bertani media - by Bioz Stars, 2020-01
    97/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Engineering ligand-responsive RNA controllers in yeast through the assembly of RNase III tuning modules
    Article Snippet: .. Ligation products were electroporated with a GenePulser XCell (Bio-Rad, Hercules, CA, USA) into Escherichia coli DH10B (Invitrogen, Carlsbad, CA), where cells harboring cloned plasmids were maintained in Luria-Bertani media containing 50 mg/ml ampicillin (EMD Chemicals). .. Clones were initially verified through colony PCR and restriction mapping.

    Article Title: De novo production of the key branch point benzylisoquinoline alkaloid reticuline in yeast
    Article Snippet: Cloning was performed with chemically competent Escherichia coli (TOP10, LifeTech, F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR ) endA1 λ- ). .. E. coli were cultured in Luria-Bertani media (EMD Chemicals) with appropriate antibiotic: 100 μg/mL ampicillin (EMD Chemicals) or 50 μg/mL kanamycin (EMD Chemicals).

    Article Title: Discovery and functional prioritization of Parkinson’s disease candidate genes from large-scale whole exome sequencing
    Article Snippet: shRNA virus production Bacterial glycerol stocks containing the shRNA vectors (Sigma, St. Louis, MO, USA; TRC1 and 1.5) were grown overnight in Luria-Bertani media containing 100 μg/mL of ampicillin (Sigma-Aldrich, St. Louis, MO, USA). .. We selected at least five shRNA clones per gene.

    Article Title: Engineering strategies for the fermentative production of plant alkaloids in yeast
    Article Snippet: Cloning was performed with chemically competent Escherichia coli (TOP10, LifeTech, F- mcrA Δ(mrr-hsdRMS-mcrBC) φp80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR ) endA1 λ− ). .. E. coli were cultured in Luria-Bertani media (EMD Chemicals) with appropriate antibiotic: 100 µg/mL ampicillin (EMD Chemicals) or 50 µg/mL kanamycin (EMD Chemicals).

    Article Title: Cloning, recombinant production, crystallization and preliminary X-ray diffraction studies of a family 84 glycoside hydrolase from Clostridium perfringens
    Article Snippet: Paragraph title: 2.1. Cloning, production and purification of GH84C ... The GH84C catalytic module was overproduced in Escherichia coli BL21 Star (DE3) (Invitrogen, Carlsbad, CA, USA) in Luria–Bertani media containing 50 µg ml−1 kanamycin (Sigma).

    Centrifugation:

    Article Title: Cloning, recombinant production, crystallization and preliminary X-ray diffraction studies of a family 84 glycoside hydrolase from Clostridium perfringens
    Article Snippet: The GH84C catalytic module was overproduced in Escherichia coli BL21 Star (DE3) (Invitrogen, Carlsbad, CA, USA) in Luria–Bertani media containing 50 µg ml−1 kanamycin (Sigma). .. Following resuspension in binding buffer (20 m M Tris pH 8.0 containing 500 m M NaCl), the cells were lysed with a French pressure cell and the cell debris was pelleted by centrifugation at 17 608 g .

    Amplification:

    Article Title: Cloning, recombinant production, crystallization and preliminary X-ray diffraction studies of a family 84 glycoside hydrolase from Clostridium perfringens
    Article Snippet: The amplified DNA fragment was cloned into pET-28a(+) (Novagen, Mississauga, ON, Canada) via the introduced Nhe I and Bam HI restriction endonuclease sites to produce pET-GH84Ccat. .. The GH84C catalytic module was overproduced in Escherichia coli BL21 Star (DE3) (Invitrogen, Carlsbad, CA, USA) in Luria–Bertani media containing 50 µg ml−1 kanamycin (Sigma).

    DNA Synthesis:

    Article Title: Engineering ligand-responsive RNA controllers in yeast through the assembly of RNase III tuning modules
    Article Snippet: DNA synthesis was performed by Integrated DNA Technologies (Coralville, IA, USA) or the Protein and Nucleic Acid Facility (Stanford, CA, USA). .. Ligation products were electroporated with a GenePulser XCell (Bio-Rad, Hercules, CA, USA) into Escherichia coli DH10B (Invitrogen, Carlsbad, CA), where cells harboring cloned plasmids were maintained in Luria-Bertani media containing 50 mg/ml ampicillin (EMD Chemicals).

    Synthesized:

    Article Title: De novo production of the key branch point benzylisoquinoline alkaloid reticuline in yeast
    Article Snippet: Oligonucleotides were synthesized by either the Stanford Protein and Nucleic Acid Facility (Stanford, CA) or Integrated DNA Technologies (Coralville, IA). .. E. coli were cultured in Luria-Bertani media (EMD Chemicals) with appropriate antibiotic: 100 μg/mL ampicillin (EMD Chemicals) or 50 μg/mL kanamycin (EMD Chemicals).

    Article Title: Engineering strategies for the fermentative production of plant alkaloids in yeast
    Article Snippet: Oligonucleotides were synthesized by either the Stanford Protein and Nucleic Acid Facility (Stanford, CA) or Integrated DNA Technologies (Coralville, IA). .. E. coli were cultured in Luria-Bertani media (EMD Chemicals) with appropriate antibiotic: 100 µg/mL ampicillin (EMD Chemicals) or 50 µg/mL kanamycin (EMD Chemicals).

    Nuclear Magnetic Resonance:

    Article Title: Millisecond Timescale Motions Connect Amino Acid Interaction Networks in Alpha Tryptophan Synthase
    Article Snippet: .. Overexpression and purification of αTS samples All samples were overexpressed using Escherichia coli BL21(DE3* ) cells grown in either Luria-Bertani media (EMD Millipore, Billerica, MA, USA) or M9 minimal media for NMR experiments. .. Samples for backbone relaxation dispersion were grown in 2 H2 O-based media with 15 N-labeled ammonium chloride (Cambridge Isotopes, Tewksbury, MA, USA).

    Article Title: Millisecond Timescale Motions Connect Amino Acid Interaction Networks in Alpha Tryptophan Synthase
    Article Snippet: .. All samples were overexpressed using Escherichia coli BL21(DE3* ) cells grown in either Luria-Bertani media (EMD Millipore, Billerica, MA, USA) or M9 minimal media for NMR experiments. .. Samples for backbone relaxation dispersion were grown in 2 H2 O-based media with 15 N-labeled ammonium chloride (Cambridge Isotopes, Tewksbury, MA, USA).

    Expressing:

    Article Title: Engineering ligand-responsive RNA controllers in yeast through the assembly of RNase III tuning modules
    Article Snippet: Ligation products were electroporated with a GenePulser XCell (Bio-Rad, Hercules, CA, USA) into Escherichia coli DH10B (Invitrogen, Carlsbad, CA), where cells harboring cloned plasmids were maintained in Luria-Bertani media containing 50 mg/ml ampicillin (EMD Chemicals). .. The construction of the Rnt1p characterization plasmid, pCS321, and the Rnt1p expression plasmid, pRNT1, have been previously described ( ).

    Filtration:

    Article Title: Millisecond Timescale Motions Connect Amino Acid Interaction Networks in Alpha Tryptophan Synthase
    Article Snippet: Overexpression and purification of αTS samples All samples were overexpressed using Escherichia coli BL21(DE3* ) cells grown in either Luria-Bertani media (EMD Millipore, Billerica, MA, USA) or M9 minimal media for NMR experiments. .. Samples were concentrated with a Corning Spin-X UF centrifugal concentrator (Sigma Aldrich, St. Louis, MO, USA) to a volume of ~1 mL then purified on a S100 gel filtration column (GE Healthcare) in buffer A with 200 mM NaCl.

    Article Title: Millisecond Timescale Motions Connect Amino Acid Interaction Networks in Alpha Tryptophan Synthase
    Article Snippet: All samples were overexpressed using Escherichia coli BL21(DE3* ) cells grown in either Luria-Bertani media (EMD Millipore, Billerica, MA, USA) or M9 minimal media for NMR experiments. .. Samples were concentrated with a Corning Spin-X UF centrifugal concentrator (Sigma Aldrich, St. Louis, MO, USA) to a volume of ~1 mL then purified on a S100 gel filtration column (GE Healthcare) in buffer A with 200 mM NaCl.

    Over Expression:

    Article Title: Millisecond Timescale Motions Connect Amino Acid Interaction Networks in Alpha Tryptophan Synthase
    Article Snippet: .. Overexpression and purification of αTS samples All samples were overexpressed using Escherichia coli BL21(DE3* ) cells grown in either Luria-Bertani media (EMD Millipore, Billerica, MA, USA) or M9 minimal media for NMR experiments. .. Samples for backbone relaxation dispersion were grown in 2 H2 O-based media with 15 N-labeled ammonium chloride (Cambridge Isotopes, Tewksbury, MA, USA).

    Article Title: Millisecond Timescale Motions Connect Amino Acid Interaction Networks in Alpha Tryptophan Synthase
    Article Snippet: Paragraph title: Overexpression and purification of αTS samples ... All samples were overexpressed using Escherichia coli BL21(DE3* ) cells grown in either Luria-Bertani media (EMD Millipore, Billerica, MA, USA) or M9 minimal media for NMR experiments.

    Derivative Assay:

    Article Title: Engineering strategies for the fermentative production of plant alkaloids in yeast
    Article Snippet: The S. cerevisiae strains described in this work are all derived from W303α (MATα leu2–3,112 trp1-1 can1–100 ura3-1 ade2-1 his3–11,15). .. E. coli were cultured in Luria-Bertani media (EMD Chemicals) with appropriate antibiotic: 100 µg/mL ampicillin (EMD Chemicals) or 50 µg/mL kanamycin (EMD Chemicals).

    Ligation:

    Article Title: Engineering ligand-responsive RNA controllers in yeast through the assembly of RNase III tuning modules
    Article Snippet: .. Ligation products were electroporated with a GenePulser XCell (Bio-Rad, Hercules, CA, USA) into Escherichia coli DH10B (Invitrogen, Carlsbad, CA), where cells harboring cloned plasmids were maintained in Luria-Bertani media containing 50 mg/ml ampicillin (EMD Chemicals). .. Clones were initially verified through colony PCR and restriction mapping.

    Cell Culture:

    Article Title: De novo production of the key branch point benzylisoquinoline alkaloid reticuline in yeast
    Article Snippet: .. E. coli were cultured in Luria-Bertani media (EMD Chemicals) with appropriate antibiotic: 100 μg/mL ampicillin (EMD Chemicals) or 50 μg/mL kanamycin (EMD Chemicals). ..

    Article Title: Discovery and functional prioritization of Parkinson’s disease candidate genes from large-scale whole exome sequencing
    Article Snippet: shRNA virus production Bacterial glycerol stocks containing the shRNA vectors (Sigma, St. Louis, MO, USA; TRC1 and 1.5) were grown overnight in Luria-Bertani media containing 100 μg/mL of ampicillin (Sigma-Aldrich, St. Louis, MO, USA). .. Lentivirus was produced as follows: HEK293T packaging cells were seeded at a density of 4 × 10Δ5 /mL (100 μL per well) in cell culture media, Optimem (Invitrogen, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) in 96-well tissue culture plates.

    Article Title: Engineering strategies for the fermentative production of plant alkaloids in yeast
    Article Snippet: .. E. coli were cultured in Luria-Bertani media (EMD Chemicals) with appropriate antibiotic: 100 µg/mL ampicillin (EMD Chemicals) or 50 µg/mL kanamycin (EMD Chemicals). ..

    Polymerase Chain Reaction:

    Article Title: Engineering ligand-responsive RNA controllers in yeast through the assembly of RNase III tuning modules
    Article Snippet: Ligation products were electroporated with a GenePulser XCell (Bio-Rad, Hercules, CA, USA) into Escherichia coli DH10B (Invitrogen, Carlsbad, CA), where cells harboring cloned plasmids were maintained in Luria-Bertani media containing 50 mg/ml ampicillin (EMD Chemicals). .. Clones were initially verified through colony PCR and restriction mapping.

    Article Title: Engineering strategies for the fermentative production of plant alkaloids in yeast
    Article Snippet: E. coli were cultured in Luria-Bertani media (EMD Chemicals) with appropriate antibiotic: 100 µg/mL ampicillin (EMD Chemicals) or 50 µg/mL kanamycin (EMD Chemicals). .. DNA polymerases used were either PfuUltraII Fusion HS DNA Polymerase (Life Technologies) or Expand High Fidelity Polymerase (Roche), and PCR products were cleaned up using QIAquick PCR purification kit (Qiagen).

    Article Title: Cloning, recombinant production, crystallization and preliminary X-ray diffraction studies of a family 84 glycoside hydrolase from Clostridium perfringens
    Article Snippet: The PCR product was obtained using Platimum Pfx polymerase (Invitrogen, Carlsbad, CA, USA) with an initial 5 min 367 K hot start followed by 30 thermal cycles of 367 K for 45 s, 317 K for 45 s and 341 K for 2 min and a final 5 min extension time at 341 K. This allowed amplification of nucleotides 91–1872 of the gh84C gene, which encode amino acids 31–624 of GH84C. .. The GH84C catalytic module was overproduced in Escherichia coli BL21 Star (DE3) (Invitrogen, Carlsbad, CA, USA) in Luria–Bertani media containing 50 µg ml−1 kanamycin (Sigma).

    Binding Assay:

    Article Title: Cloning, recombinant production, crystallization and preliminary X-ray diffraction studies of a family 84 glycoside hydrolase from Clostridium perfringens
    Article Snippet: The GH84C catalytic module was overproduced in Escherichia coli BL21 Star (DE3) (Invitrogen, Carlsbad, CA, USA) in Luria–Bertani media containing 50 µg ml−1 kanamycin (Sigma). .. Following resuspension in binding buffer (20 m M Tris pH 8.0 containing 500 m M NaCl), the cells were lysed with a French pressure cell and the cell debris was pelleted by centrifugation at 17 608 g .

    Purification:

    Article Title: Millisecond Timescale Motions Connect Amino Acid Interaction Networks in Alpha Tryptophan Synthase
    Article Snippet: .. Overexpression and purification of αTS samples All samples were overexpressed using Escherichia coli BL21(DE3* ) cells grown in either Luria-Bertani media (EMD Millipore, Billerica, MA, USA) or M9 minimal media for NMR experiments. .. Samples for backbone relaxation dispersion were grown in 2 H2 O-based media with 15 N-labeled ammonium chloride (Cambridge Isotopes, Tewksbury, MA, USA).

    Article Title: Engineering strategies for the fermentative production of plant alkaloids in yeast
    Article Snippet: E. coli were cultured in Luria-Bertani media (EMD Chemicals) with appropriate antibiotic: 100 µg/mL ampicillin (EMD Chemicals) or 50 µg/mL kanamycin (EMD Chemicals). .. DNA polymerases used were either PfuUltraII Fusion HS DNA Polymerase (Life Technologies) or Expand High Fidelity Polymerase (Roche), and PCR products were cleaned up using QIAquick PCR purification kit (Qiagen).

    Article Title: Millisecond Timescale Motions Connect Amino Acid Interaction Networks in Alpha Tryptophan Synthase
    Article Snippet: Paragraph title: Overexpression and purification of αTS samples ... All samples were overexpressed using Escherichia coli BL21(DE3* ) cells grown in either Luria-Bertani media (EMD Millipore, Billerica, MA, USA) or M9 minimal media for NMR experiments.

    Article Title: Cloning, recombinant production, crystallization and preliminary X-ray diffraction studies of a family 84 glycoside hydrolase from Clostridium perfringens
    Article Snippet: Paragraph title: 2.1. Cloning, production and purification of GH84C ... The GH84C catalytic module was overproduced in Escherichia coli BL21 Star (DE3) (Invitrogen, Carlsbad, CA, USA) in Luria–Bertani media containing 50 µg ml−1 kanamycin (Sigma).

    Sequencing:

    Article Title: Engineering ligand-responsive RNA controllers in yeast through the assembly of RNase III tuning modules
    Article Snippet: Ligation products were electroporated with a GenePulser XCell (Bio-Rad, Hercules, CA, USA) into Escherichia coli DH10B (Invitrogen, Carlsbad, CA), where cells harboring cloned plasmids were maintained in Luria-Bertani media containing 50 mg/ml ampicillin (EMD Chemicals). .. All cloned constructs were sequence verified by Laragen (Los Angeles, CA, USA) or Elim Biopharmaceuticals (Hayward, CA, USA).

    Article Title: Cloning, recombinant production, crystallization and preliminary X-ray diffraction studies of a family 84 glycoside hydrolase from Clostridium perfringens
    Article Snippet: The encoded polypeptide comprises an N-terminal six-histidine tag separated from the GH84 module by a thrombin-cleavage sequence. .. The GH84C catalytic module was overproduced in Escherichia coli BL21 Star (DE3) (Invitrogen, Carlsbad, CA, USA) in Luria–Bertani media containing 50 µg ml−1 kanamycin (Sigma).

    Positron Emission Tomography:

    Article Title: Cloning, recombinant production, crystallization and preliminary X-ray diffraction studies of a family 84 glycoside hydrolase from Clostridium perfringens
    Article Snippet: The amplified DNA fragment was cloned into pET-28a(+) (Novagen, Mississauga, ON, Canada) via the introduced Nhe I and Bam HI restriction endonuclease sites to produce pET-GH84Ccat. .. The GH84C catalytic module was overproduced in Escherichia coli BL21 Star (DE3) (Invitrogen, Carlsbad, CA, USA) in Luria–Bertani media containing 50 µg ml−1 kanamycin (Sigma).

    IA:

    Article Title: Engineering ligand-responsive RNA controllers in yeast through the assembly of RNase III tuning modules
    Article Snippet: DNA synthesis was performed by Integrated DNA Technologies (Coralville, IA, USA) or the Protein and Nucleic Acid Facility (Stanford, CA, USA). .. Ligation products were electroporated with a GenePulser XCell (Bio-Rad, Hercules, CA, USA) into Escherichia coli DH10B (Invitrogen, Carlsbad, CA), where cells harboring cloned plasmids were maintained in Luria-Bertani media containing 50 mg/ml ampicillin (EMD Chemicals).

    Article Title: De novo production of the key branch point benzylisoquinoline alkaloid reticuline in yeast
    Article Snippet: Oligonucleotides were synthesized by either the Stanford Protein and Nucleic Acid Facility (Stanford, CA) or Integrated DNA Technologies (Coralville, IA). .. E. coli were cultured in Luria-Bertani media (EMD Chemicals) with appropriate antibiotic: 100 μg/mL ampicillin (EMD Chemicals) or 50 μg/mL kanamycin (EMD Chemicals).

    Article Title: Engineering strategies for the fermentative production of plant alkaloids in yeast
    Article Snippet: Oligonucleotides were synthesized by either the Stanford Protein and Nucleic Acid Facility (Stanford, CA) or Integrated DNA Technologies (Coralville, IA). .. E. coli were cultured in Luria-Bertani media (EMD Chemicals) with appropriate antibiotic: 100 µg/mL ampicillin (EMD Chemicals) or 50 µg/mL kanamycin (EMD Chemicals).

    Activated Clotting Time Assay:

    Article Title: Cloning, recombinant production, crystallization and preliminary X-ray diffraction studies of a family 84 glycoside hydrolase from Clostridium perfringens
    Article Snippet: The DNA fragment encoding the GH84C catalytic module was amplified from C. perfringens genomic DNA (strain ATCC 13124; purchased from Sigma, St Louis, MO, USA) by PCR using the oligonucleotide primers 5′-CAT ATG GCT AGC GTA GGA CCT AAA ACT GGG-3′ and 5′-GAA TTC GGA TCC TTA TAT TAA TGT TAA ATC AAA ACT TAA AGC-3′ (Qiagen, Mississauga, ON, Canada). .. The GH84C catalytic module was overproduced in Escherichia coli BL21 Star (DE3) (Invitrogen, Carlsbad, CA, USA) in Luria–Bertani media containing 50 µg ml−1 kanamycin (Sigma).

    SDS Page:

    Article Title: Cloning, recombinant production, crystallization and preliminary X-ray diffraction studies of a family 84 glycoside hydrolase from Clostridium perfringens
    Article Snippet: The GH84C catalytic module was overproduced in Escherichia coli BL21 Star (DE3) (Invitrogen, Carlsbad, CA, USA) in Luria–Bertani media containing 50 µg ml−1 kanamycin (Sigma). .. Fractions were electrophoresed by SDS–PAGE and those fractions containing protein judged to be greater than 95% pure were pooled, concentrated and buffer-exchanged into 20 m M Tris pH 8.0 using a stirred ultrafiltration cell (Amicon) with a 3.5 kDa cutoff membrane (Fisher, Nepean, ON, Canada).

    Plasmid Preparation:

    Article Title: Engineering ligand-responsive RNA controllers in yeast through the assembly of RNase III tuning modules
    Article Snippet: Paragraph title: Plasmid construction ... Ligation products were electroporated with a GenePulser XCell (Bio-Rad, Hercules, CA, USA) into Escherichia coli DH10B (Invitrogen, Carlsbad, CA), where cells harboring cloned plasmids were maintained in Luria-Bertani media containing 50 mg/ml ampicillin (EMD Chemicals).

    Article Title: De novo production of the key branch point benzylisoquinoline alkaloid reticuline in yeast
    Article Snippet: Paragraph title: 1.2.1 Yeast strain and plasmid construction ... E. coli were cultured in Luria-Bertani media (EMD Chemicals) with appropriate antibiotic: 100 μg/mL ampicillin (EMD Chemicals) or 50 μg/mL kanamycin (EMD Chemicals).

    Article Title: Discovery and functional prioritization of Parkinson’s disease candidate genes from large-scale whole exome sequencing
    Article Snippet: shRNA virus production Bacterial glycerol stocks containing the shRNA vectors (Sigma, St. Louis, MO, USA; TRC1 and 1.5) were grown overnight in Luria-Bertani media containing 100 μg/mL of ampicillin (Sigma-Aldrich, St. Louis, MO, USA). .. Endotoxin-free shRNA plasmids were extracted according to the manufacturer’s protocol (Zymo, Irvine, CA, USA; ZR Plasmid Miniprep Classic kit).

    Article Title: Engineering strategies for the fermentative production of plant alkaloids in yeast
    Article Snippet: Paragraph title: 2.1 Plasmid and yeast strain construction ... E. coli were cultured in Luria-Bertani media (EMD Chemicals) with appropriate antibiotic: 100 µg/mL ampicillin (EMD Chemicals) or 50 µg/mL kanamycin (EMD Chemicals).

    shRNA:

    Article Title: Discovery and functional prioritization of Parkinson’s disease candidate genes from large-scale whole exome sequencing
    Article Snippet: .. shRNA virus production Bacterial glycerol stocks containing the shRNA vectors (Sigma, St. Louis, MO, USA; TRC1 and 1.5) were grown overnight in Luria-Bertani media containing 100 μg/mL of ampicillin (Sigma-Aldrich, St. Louis, MO, USA). .. We selected at least five shRNA clones per gene.

    Transgenic Assay:

    Article Title: The Role of NF-κB Factor REL2 in the Aedes aegypti Immune Response
    Article Snippet: To conduct survival experiments for REL2 transgenic mosquitoes, 4-day-old females were blood fed on White Leghorn chickens and then bacteria challenged at 6–10 h PBM. .. Bacteria Pseudomonas aeruginosa (ATCC 012406/10145) and Enterobacter cloacae were grown in Luria Bertani media (Sigma-Aldrich, St-Louis, MO, USA), and the following concentrations were used for bacterial challenge: P. aeruginosa OD600 = 0.6, E. cloacae OD600 = 2.8.

    Produced:

    Article Title: Discovery and functional prioritization of Parkinson’s disease candidate genes from large-scale whole exome sequencing
    Article Snippet: shRNA virus production Bacterial glycerol stocks containing the shRNA vectors (Sigma, St. Louis, MO, USA; TRC1 and 1.5) were grown overnight in Luria-Bertani media containing 100 μg/mL of ampicillin (Sigma-Aldrich, St. Louis, MO, USA). .. Lentivirus was produced as follows: HEK293T packaging cells were seeded at a density of 4 × 10Δ5 /mL (100 μL per well) in cell culture media, Optimem (Invitrogen, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) in 96-well tissue culture plates.

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  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    Millipore luria bertani media
    Luria Bertani Media, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luria bertani media/product/Millipore
    Average 97 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    luria bertani media - by Bioz Stars, 2020-01
    97/100 stars
      Buy from Supplier

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