luria bertani media  (Millipore)


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    Structured Review

    Millipore luria bertani media
    Luria Bertani Media, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luria bertani media/product/Millipore
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    luria bertani media - by Bioz Stars, 2020-04
    94/100 stars

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    Related Articles

    Clone Assay:

    Article Title: A high-throughput, quantitative cell-based screen for efficient tailoring of RNA device activity
    Article Snippet: .. Ligation products were electroporated with a GenePulser XCell (Bio-Rad, Hercules, CA) into an Escherichia coli DH10B strain (Invitrogen, Carlsbad, CA), where cells harboring cloned plasmids were maintained in Luria-Bertani media containing 50 µg/mL ampicillin (EMD Chemicals, Philadelphia, PA). ..

    Article Title: Engineering ligand-responsive RNA controllers in yeast through the assembly of RNase III tuning modules
    Article Snippet: .. Ligation products were electroporated with a GenePulser XCell (Bio-Rad, Hercules, CA, USA) into Escherichia coli DH10B (Invitrogen, Carlsbad, CA), where cells harboring cloned plasmids were maintained in Luria-Bertani media containing 50 mg/ml ampicillin (EMD Chemicals). .. Clones were initially verified through colony PCR and restriction mapping.

    Article Title: De novo production of the key branch point benzylisoquinoline alkaloid reticuline in yeast
    Article Snippet: Cloning was performed with chemically competent Escherichia coli (TOP10, LifeTech, F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR ) endA1 λ- ). .. E. coli were cultured in Luria-Bertani media (EMD Chemicals) with appropriate antibiotic: 100 μg/mL ampicillin (EMD Chemicals) or 50 μg/mL kanamycin (EMD Chemicals).

    Article Title: Crystallization and preliminary X-ray diffraction studies of a protein disulfide oxidoreductase from Aeropyrum pernix K1
    Article Snippet: The Ap PDO-encoding gene was amplified by PCR and cloned between the Nco I and Bam HI sites of the pTrc99A vector (Novagen). .. Cells were grown to an OD600 of approximately 1 in Luria–Bertani media containing 0.1 mg ml−1 ampicillin (Sigma) at 310 K and the expression of Ap PDO was induced by 1 m M isopropyl-β- d -thiogalactoside (IPTG; Inalco).

    Article Title: Engineering strategies for the fermentative production of plant alkaloids in yeast
    Article Snippet: Cloning was performed with chemically competent Escherichia coli (TOP10, LifeTech, F- mcrA Δ(mrr-hsdRMS-mcrBC) φp80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR ) endA1 λ− ). .. E. coli were cultured in Luria-Bertani media (EMD Chemicals) with appropriate antibiotic: 100 µg/mL ampicillin (EMD Chemicals) or 50 µg/mL kanamycin (EMD Chemicals).

    Article Title: A synthetic library of RNA control modules for predictable tuning of gene expression in yeast
    Article Snippet: .. Ligation products were electroporated into Escherichia coli DH10B (Invitrogen, Carlsbad, CA), and cells harboring cloned plasmids were maintained in Luria–Bertani media containing 50 mg/ml ampicillin (EMD Chemicals). .. Clones were initially verified through colony PCR and restriction mapping.

    Centrifugation:

    Article Title: Crystallization and preliminary X-ray diffraction studies of a protein disulfide oxidoreductase from Aeropyrum pernix K1
    Article Snippet: Cells were grown to an OD600 of approximately 1 in Luria–Bertani media containing 0.1 mg ml−1 ampicillin (Sigma) at 310 K and the expression of Ap PDO was induced by 1 m M isopropyl-β- d -thiogalactoside (IPTG; Inalco). .. After sonication and subsequent centrifugation, the cell extract was heated and kept at 353 K for 10 min. After centrifugation at 35 000 g for 30 min, the supernatant was loaded onto a S75 Superdex gel-filtration column (Amersham Pharmacia Biotech) previously equilibrated with buffer A containing 200 m M KCl.

    Amplification:

    Article Title: A high-throughput, quantitative cell-based screen for efficient tailoring of RNA device activity
    Article Snippet: Ligation products were electroporated with a GenePulser XCell (Bio-Rad, Hercules, CA) into an Escherichia coli DH10B strain (Invitrogen, Carlsbad, CA), where cells harboring cloned plasmids were maintained in Luria-Bertani media containing 50 µg/mL ampicillin (EMD Chemicals, Philadelphia, PA). .. The TEF1 promoter was polymerase chain reaction (PCR) amplified from pG2M ( ) using forward and reverse primers SacI-TEF1-fwd (5′-GAGAGCTCAAGCTTCAAAATGTTTCTACTCC) and SacII-TEF1-rev (5′-GGCCGCGGCAAAACTTAGATTAGATTGCTATGC), respectively, and inserted into pCS321 via the unique restriction sites SacI and SacII.

    Article Title: Crystallization and preliminary X-ray diffraction studies of a protein disulfide oxidoreductase from Aeropyrum pernix K1
    Article Snippet: The Ap PDO-encoding gene was amplified by PCR and cloned between the Nco I and Bam HI sites of the pTrc99A vector (Novagen). .. Cells were grown to an OD600 of approximately 1 in Luria–Bertani media containing 0.1 mg ml−1 ampicillin (Sigma) at 310 K and the expression of Ap PDO was induced by 1 m M isopropyl-β- d -thiogalactoside (IPTG; Inalco).

    DNA Synthesis:

    Article Title: A high-throughput, quantitative cell-based screen for efficient tailoring of RNA device activity
    Article Snippet: DNA synthesis was performed by Integrated DNA Technologies (Coralville, IA) or the Protein and Nucleic Acid Facility (Stanford, CA). .. Ligation products were electroporated with a GenePulser XCell (Bio-Rad, Hercules, CA) into an Escherichia coli DH10B strain (Invitrogen, Carlsbad, CA), where cells harboring cloned plasmids were maintained in Luria-Bertani media containing 50 µg/mL ampicillin (EMD Chemicals, Philadelphia, PA).

    Article Title: Engineering ligand-responsive RNA controllers in yeast through the assembly of RNase III tuning modules
    Article Snippet: DNA synthesis was performed by Integrated DNA Technologies (Coralville, IA, USA) or the Protein and Nucleic Acid Facility (Stanford, CA, USA). .. Ligation products were electroporated with a GenePulser XCell (Bio-Rad, Hercules, CA, USA) into Escherichia coli DH10B (Invitrogen, Carlsbad, CA), where cells harboring cloned plasmids were maintained in Luria-Bertani media containing 50 mg/ml ampicillin (EMD Chemicals).

    Article Title: A synthetic library of RNA control modules for predictable tuning of gene expression in yeast
    Article Snippet: DNA synthesis was performed by Integrated DNA Technologies (Coralville, IA) or the Protein and Nucleic Acid Facility (Stanford, CA). .. Ligation products were electroporated into Escherichia coli DH10B (Invitrogen, Carlsbad, CA), and cells harboring cloned plasmids were maintained in Luria–Bertani media containing 50 mg/ml ampicillin (EMD Chemicals).

    Synthesized:

    Article Title: De novo production of the key branch point benzylisoquinoline alkaloid reticuline in yeast
    Article Snippet: Oligonucleotides were synthesized by either the Stanford Protein and Nucleic Acid Facility (Stanford, CA) or Integrated DNA Technologies (Coralville, IA). .. E. coli were cultured in Luria-Bertani media (EMD Chemicals) with appropriate antibiotic: 100 μg/mL ampicillin (EMD Chemicals) or 50 μg/mL kanamycin (EMD Chemicals).

    Article Title: Mechanical stability of αT-catenin and its activation by force for vinculin binding
    Article Snippet: Protein expression Two fragments of the αT-catenin middle domains (M123: 259–667; M1: 259–295) were synthesized by PCR using plasmid template containing the full-length αT-catenin sequence. .. Subsequently, the proteins of interest were expressed in Escherichia coli BL21 (DE3) cultured in Luria-Bertani media with D-Biotin (Sigma-Aldrich) and affinity purified using the His-tag.

    Article Title: Engineering strategies for the fermentative production of plant alkaloids in yeast
    Article Snippet: Oligonucleotides were synthesized by either the Stanford Protein and Nucleic Acid Facility (Stanford, CA) or Integrated DNA Technologies (Coralville, IA). .. E. coli were cultured in Luria-Bertani media (EMD Chemicals) with appropriate antibiotic: 100 µg/mL ampicillin (EMD Chemicals) or 50 µg/mL kanamycin (EMD Chemicals).

    Incubation:

    Article Title: Roles for Drosophila melanogaster Myosin IB in Maintenance of Enterocyte Brush-Border Structure and Resistance to the Bacterial Pathogen Pseudomonas entomophila
    Article Snippet: P. entomophila was grown for 24 h in Luria-Bertani media supplemented with 50 μg/ml rifampicin (Sigma Aldrich, St. Louis, MO) at 29°C. .. The larvae were then transferred to fresh apple juice plates and incubated at 29°C.

    Cell Culture:

    Article Title: De novo production of the key branch point benzylisoquinoline alkaloid reticuline in yeast
    Article Snippet: .. E. coli were cultured in Luria-Bertani media (EMD Chemicals) with appropriate antibiotic: 100 μg/mL ampicillin (EMD Chemicals) or 50 μg/mL kanamycin (EMD Chemicals). ..

    Article Title: Mechanical stability of αT-catenin and its activation by force for vinculin binding
    Article Snippet: .. Subsequently, the proteins of interest were expressed in Escherichia coli BL21 (DE3) cultured in Luria-Bertani media with D-Biotin (Sigma-Aldrich) and affinity purified using the His-tag. .. Single-molecule manipulation The single-molecule manipulation experiments were carried out on a custom-built magnetic tweezers platform that can exert forces up to 100 pN with ∼1 nm extension resolution for tethered bead at a 200 Hz sampling rate ( ; ).

    Article Title: Engineering strategies for the fermentative production of plant alkaloids in yeast
    Article Snippet: .. E. coli were cultured in Luria-Bertani media (EMD Chemicals) with appropriate antibiotic: 100 µg/mL ampicillin (EMD Chemicals) or 50 µg/mL kanamycin (EMD Chemicals). ..

    Expressing:

    Article Title: Engineering ligand-responsive RNA controllers in yeast through the assembly of RNase III tuning modules
    Article Snippet: Ligation products were electroporated with a GenePulser XCell (Bio-Rad, Hercules, CA, USA) into Escherichia coli DH10B (Invitrogen, Carlsbad, CA), where cells harboring cloned plasmids were maintained in Luria-Bertani media containing 50 mg/ml ampicillin (EMD Chemicals). .. The construction of the Rnt1p characterization plasmid, pCS321, and the Rnt1p expression plasmid, pRNT1, have been previously described ( ).

    Article Title: Crystallization and preliminary X-ray diffraction studies of a protein disulfide oxidoreductase from Aeropyrum pernix K1
    Article Snippet: .. Cells were grown to an OD600 of approximately 1 in Luria–Bertani media containing 0.1 mg ml−1 ampicillin (Sigma) at 310 K and the expression of Ap PDO was induced by 1 m M isopropyl-β- d -thiogalactoside (IPTG; Inalco). ..

    Article Title: Mechanical stability of αT-catenin and its activation by force for vinculin binding
    Article Snippet: Paragraph title: Protein expression ... Subsequently, the proteins of interest were expressed in Escherichia coli BL21 (DE3) cultured in Luria-Bertani media with D-Biotin (Sigma-Aldrich) and affinity purified using the His-tag.

    Filtration:

    Article Title: Millisecond Timescale Motions Connect Amino Acid Interaction Networks in Alpha Tryptophan Synthase
    Article Snippet: Overexpression and purification of αTS samples All samples were overexpressed using Escherichia coli BL21(DE3* ) cells grown in either Luria-Bertani media (EMD Millipore, Billerica, MA, USA) or M9 minimal media for NMR experiments. .. Samples were concentrated with a Corning Spin-X UF centrifugal concentrator (Sigma Aldrich, St. Louis, MO, USA) to a volume of ~1 mL then purified on a S100 gel filtration column (GE Healthcare) in buffer A with 200 mM NaCl.

    Article Title: Millisecond Timescale Motions Connect Amino Acid Interaction Networks in Alpha Tryptophan Synthase
    Article Snippet: All samples were overexpressed using Escherichia coli BL21(DE3* ) cells grown in either Luria-Bertani media (EMD Millipore, Billerica, MA, USA) or M9 minimal media for NMR experiments. .. Samples were concentrated with a Corning Spin-X UF centrifugal concentrator (Sigma Aldrich, St. Louis, MO, USA) to a volume of ~1 mL then purified on a S100 gel filtration column (GE Healthcare) in buffer A with 200 mM NaCl.

    Transformation Assay:

    Article Title: The Role of Ceramide Synthases in the Pathogenicity of Cryptococcus neoformans
    Article Snippet: S. cerevisiae transformed with pYES2/CT was grown in YNB (ura− ), and S. cerevisiae transformed with pRS425 was grown in synthetic leucine (leu− ) dropout media. .. Bacterial strains were grown at 37°C in Luria-Bertani media containing 75 mg/L of ampicillin (Sigma-Aldrich).

    Article Title: Crystallization and preliminary X-ray diffraction studies of a protein disulfide oxidoreductase from Aeropyrum pernix K1
    Article Snippet: This vector was then transformed into Escherichia coli strain RB791. .. Cells were grown to an OD600 of approximately 1 in Luria–Bertani media containing 0.1 mg ml−1 ampicillin (Sigma) at 310 K and the expression of Ap PDO was induced by 1 m M isopropyl-β- d -thiogalactoside (IPTG; Inalco).

    Over Expression:

    Article Title: The Role of Ceramide Synthases in the Pathogenicity of Cryptococcus neoformans
    Article Snippet: The three putative ceramide synthase genes in this study have the following identifiers: CNAG_06717 (GenBank: ), CNAG_02086 (GenBank: ), and CNAG_02087 (GenBank: ). pYES2/CT was used for overexpression of CNAG_06717 in S. cerevisiae BY4741 and pRS425 for CNAG_02086 and CNAG_02087. .. Bacterial strains were grown at 37°C in Luria-Bertani media containing 75 mg/L of ampicillin (Sigma-Aldrich).

    Article Title: Millisecond Timescale Motions Connect Amino Acid Interaction Networks in Alpha Tryptophan Synthase
    Article Snippet: .. Overexpression and purification of αTS samples All samples were overexpressed using Escherichia coli BL21(DE3* ) cells grown in either Luria-Bertani media (EMD Millipore, Billerica, MA, USA) or M9 minimal media for NMR experiments. .. Samples for backbone relaxation dispersion were grown in 2 H2 O-based media with 15 N-labeled ammonium chloride (Cambridge Isotopes, Tewksbury, MA, USA).

    Article Title: Millisecond Timescale Motions Connect Amino Acid Interaction Networks in Alpha Tryptophan Synthase
    Article Snippet: Paragraph title: Overexpression and purification of αTS samples ... All samples were overexpressed using Escherichia coli BL21(DE3* ) cells grown in either Luria-Bertani media (EMD Millipore, Billerica, MA, USA) or M9 minimal media for NMR experiments.

    Derivative Assay:

    Article Title: Engineering strategies for the fermentative production of plant alkaloids in yeast
    Article Snippet: The S. cerevisiae strains described in this work are all derived from W303α (MATα leu2–3,112 trp1-1 can1–100 ura3-1 ade2-1 his3–11,15). .. E. coli were cultured in Luria-Bertani media (EMD Chemicals) with appropriate antibiotic: 100 µg/mL ampicillin (EMD Chemicals) or 50 µg/mL kanamycin (EMD Chemicals).

    Ligation:

    Article Title: A high-throughput, quantitative cell-based screen for efficient tailoring of RNA device activity
    Article Snippet: .. Ligation products were electroporated with a GenePulser XCell (Bio-Rad, Hercules, CA) into an Escherichia coli DH10B strain (Invitrogen, Carlsbad, CA), where cells harboring cloned plasmids were maintained in Luria-Bertani media containing 50 µg/mL ampicillin (EMD Chemicals, Philadelphia, PA). ..

    Article Title: Engineering ligand-responsive RNA controllers in yeast through the assembly of RNase III tuning modules
    Article Snippet: .. Ligation products were electroporated with a GenePulser XCell (Bio-Rad, Hercules, CA, USA) into Escherichia coli DH10B (Invitrogen, Carlsbad, CA), where cells harboring cloned plasmids were maintained in Luria-Bertani media containing 50 mg/ml ampicillin (EMD Chemicals). .. Clones were initially verified through colony PCR and restriction mapping.

    Article Title: A synthetic library of RNA control modules for predictable tuning of gene expression in yeast
    Article Snippet: .. Ligation products were electroporated into Escherichia coli DH10B (Invitrogen, Carlsbad, CA), and cells harboring cloned plasmids were maintained in Luria–Bertani media containing 50 mg/ml ampicillin (EMD Chemicals). .. Clones were initially verified through colony PCR and restriction mapping.

    Infection:

    Article Title: Roles for Drosophila melanogaster Myosin IB in Maintenance of Enterocyte Brush-Border Structure and Resistance to the Bacterial Pathogen Pseudomonas entomophila
    Article Snippet: Paragraph title: Bacterial Infection ... P. entomophila was grown for 24 h in Luria-Bertani media supplemented with 50 μg/ml rifampicin (Sigma Aldrich, St. Louis, MO) at 29°C.

    other:

    Article Title: Sense overlapping transcripts in IS1341-type transposase genes are functional non-coding RNAs in archaea
    Article Snippet: E. coli strain DH5α was grown at 37°C with air shaking at 225 rpm in Luria-Bertani media and supplemented with 50 μg/mL of carbenicilin (Sigma) when necessary.

    Polymerase Chain Reaction:

    Article Title: A high-throughput, quantitative cell-based screen for efficient tailoring of RNA device activity
    Article Snippet: Ligation products were electroporated with a GenePulser XCell (Bio-Rad, Hercules, CA) into an Escherichia coli DH10B strain (Invitrogen, Carlsbad, CA), where cells harboring cloned plasmids were maintained in Luria-Bertani media containing 50 µg/mL ampicillin (EMD Chemicals, Philadelphia, PA). .. The TEF1 promoter was polymerase chain reaction (PCR) amplified from pG2M ( ) using forward and reverse primers SacI-TEF1-fwd (5′-GAGAGCTCAAGCTTCAAAATGTTTCTACTCC) and SacII-TEF1-rev (5′-GGCCGCGGCAAAACTTAGATTAGATTGCTATGC), respectively, and inserted into pCS321 via the unique restriction sites SacI and SacII.

    Article Title: Engineering ligand-responsive RNA controllers in yeast through the assembly of RNase III tuning modules
    Article Snippet: Ligation products were electroporated with a GenePulser XCell (Bio-Rad, Hercules, CA, USA) into Escherichia coli DH10B (Invitrogen, Carlsbad, CA), where cells harboring cloned plasmids were maintained in Luria-Bertani media containing 50 mg/ml ampicillin (EMD Chemicals). .. Clones were initially verified through colony PCR and restriction mapping.

    Article Title: Crystallization and preliminary X-ray diffraction studies of a protein disulfide oxidoreductase from Aeropyrum pernix K1
    Article Snippet: The Ap PDO-encoding gene was amplified by PCR and cloned between the Nco I and Bam HI sites of the pTrc99A vector (Novagen). .. Cells were grown to an OD600 of approximately 1 in Luria–Bertani media containing 0.1 mg ml−1 ampicillin (Sigma) at 310 K and the expression of Ap PDO was induced by 1 m M isopropyl-β- d -thiogalactoside (IPTG; Inalco).

    Article Title: Mechanical stability of αT-catenin and its activation by force for vinculin binding
    Article Snippet: Protein expression Two fragments of the αT-catenin middle domains (M123: 259–667; M1: 259–295) were synthesized by PCR using plasmid template containing the full-length αT-catenin sequence. .. Subsequently, the proteins of interest were expressed in Escherichia coli BL21 (DE3) cultured in Luria-Bertani media with D-Biotin (Sigma-Aldrich) and affinity purified using the His-tag.

    Article Title: Engineering strategies for the fermentative production of plant alkaloids in yeast
    Article Snippet: E. coli were cultured in Luria-Bertani media (EMD Chemicals) with appropriate antibiotic: 100 µg/mL ampicillin (EMD Chemicals) or 50 µg/mL kanamycin (EMD Chemicals). .. DNA polymerases used were either PfuUltraII Fusion HS DNA Polymerase (Life Technologies) or Expand High Fidelity Polymerase (Roche), and PCR products were cleaned up using QIAquick PCR purification kit (Qiagen).

    Article Title: A synthetic library of RNA control modules for predictable tuning of gene expression in yeast
    Article Snippet: Ligation products were electroporated into Escherichia coli DH10B (Invitrogen, Carlsbad, CA), and cells harboring cloned plasmids were maintained in Luria–Bertani media containing 50 mg/ml ampicillin (EMD Chemicals). .. Clones were initially verified through colony PCR and restriction mapping.

    Sonication:

    Article Title: Crystallization and preliminary X-ray diffraction studies of a protein disulfide oxidoreductase from Aeropyrum pernix K1
    Article Snippet: Cells were grown to an OD600 of approximately 1 in Luria–Bertani media containing 0.1 mg ml−1 ampicillin (Sigma) at 310 K and the expression of Ap PDO was induced by 1 m M isopropyl-β- d -thiogalactoside (IPTG; Inalco). .. After sonication and subsequent centrifugation, the cell extract was heated and kept at 353 K for 10 min. After centrifugation at 35 000 g for 30 min, the supernatant was loaded onto a S75 Superdex gel-filtration column (Amersham Pharmacia Biotech) previously equilibrated with buffer A containing 200 m M KCl.

    Affinity Purification:

    Article Title: Mechanical stability of αT-catenin and its activation by force for vinculin binding
    Article Snippet: .. Subsequently, the proteins of interest were expressed in Escherichia coli BL21 (DE3) cultured in Luria-Bertani media with D-Biotin (Sigma-Aldrich) and affinity purified using the His-tag. .. Single-molecule manipulation The single-molecule manipulation experiments were carried out on a custom-built magnetic tweezers platform that can exert forces up to 100 pN with ∼1 nm extension resolution for tethered bead at a 200 Hz sampling rate ( ; ).

    Purification:

    Article Title: Crystallization and preliminary X-ray diffraction studies of a protein disulfide oxidoreductase from Aeropyrum pernix K1
    Article Snippet: Paragraph title: 2.1. Expression and purification ... Cells were grown to an OD600 of approximately 1 in Luria–Bertani media containing 0.1 mg ml−1 ampicillin (Sigma) at 310 K and the expression of Ap PDO was induced by 1 m M isopropyl-β- d -thiogalactoside (IPTG; Inalco).

    Article Title: Millisecond Timescale Motions Connect Amino Acid Interaction Networks in Alpha Tryptophan Synthase
    Article Snippet: .. Overexpression and purification of αTS samples All samples were overexpressed using Escherichia coli BL21(DE3* ) cells grown in either Luria-Bertani media (EMD Millipore, Billerica, MA, USA) or M9 minimal media for NMR experiments. .. Samples for backbone relaxation dispersion were grown in 2 H2 O-based media with 15 N-labeled ammonium chloride (Cambridge Isotopes, Tewksbury, MA, USA).

    Article Title: Engineering strategies for the fermentative production of plant alkaloids in yeast
    Article Snippet: E. coli were cultured in Luria-Bertani media (EMD Chemicals) with appropriate antibiotic: 100 µg/mL ampicillin (EMD Chemicals) or 50 µg/mL kanamycin (EMD Chemicals). .. DNA polymerases used were either PfuUltraII Fusion HS DNA Polymerase (Life Technologies) or Expand High Fidelity Polymerase (Roche), and PCR products were cleaned up using QIAquick PCR purification kit (Qiagen).

    Article Title: Millisecond Timescale Motions Connect Amino Acid Interaction Networks in Alpha Tryptophan Synthase
    Article Snippet: Paragraph title: Overexpression and purification of αTS samples ... All samples were overexpressed using Escherichia coli BL21(DE3* ) cells grown in either Luria-Bertani media (EMD Millipore, Billerica, MA, USA) or M9 minimal media for NMR experiments.

    Sequencing:

    Article Title: Engineering ligand-responsive RNA controllers in yeast through the assembly of RNase III tuning modules
    Article Snippet: Ligation products were electroporated with a GenePulser XCell (Bio-Rad, Hercules, CA, USA) into Escherichia coli DH10B (Invitrogen, Carlsbad, CA), where cells harboring cloned plasmids were maintained in Luria-Bertani media containing 50 mg/ml ampicillin (EMD Chemicals). .. All cloned constructs were sequence verified by Laragen (Los Angeles, CA, USA) or Elim Biopharmaceuticals (Hayward, CA, USA).

    Article Title: Mechanical stability of αT-catenin and its activation by force for vinculin binding
    Article Snippet: Protein expression Two fragments of the αT-catenin middle domains (M123: 259–667; M1: 259–295) were synthesized by PCR using plasmid template containing the full-length αT-catenin sequence. .. Subsequently, the proteins of interest were expressed in Escherichia coli BL21 (DE3) cultured in Luria-Bertani media with D-Biotin (Sigma-Aldrich) and affinity purified using the His-tag.

    Article Title: A synthetic library of RNA control modules for predictable tuning of gene expression in yeast
    Article Snippet: Ligation products were electroporated into Escherichia coli DH10B (Invitrogen, Carlsbad, CA), and cells harboring cloned plasmids were maintained in Luria–Bertani media containing 50 mg/ml ampicillin (EMD Chemicals). .. All cloned constructs and chromosomal integrations were sequence verified by Laragen (Los Angeles, CA) or the Protein and Nucleic Acid Facility (Stanford, CA).

    IA:

    Article Title: A high-throughput, quantitative cell-based screen for efficient tailoring of RNA device activity
    Article Snippet: DNA synthesis was performed by Integrated DNA Technologies (Coralville, IA) or the Protein and Nucleic Acid Facility (Stanford, CA). .. Ligation products were electroporated with a GenePulser XCell (Bio-Rad, Hercules, CA) into an Escherichia coli DH10B strain (Invitrogen, Carlsbad, CA), where cells harboring cloned plasmids were maintained in Luria-Bertani media containing 50 µg/mL ampicillin (EMD Chemicals, Philadelphia, PA).

    Article Title: Engineering ligand-responsive RNA controllers in yeast through the assembly of RNase III tuning modules
    Article Snippet: DNA synthesis was performed by Integrated DNA Technologies (Coralville, IA, USA) or the Protein and Nucleic Acid Facility (Stanford, CA, USA). .. Ligation products were electroporated with a GenePulser XCell (Bio-Rad, Hercules, CA, USA) into Escherichia coli DH10B (Invitrogen, Carlsbad, CA), where cells harboring cloned plasmids were maintained in Luria-Bertani media containing 50 mg/ml ampicillin (EMD Chemicals).

    Article Title: De novo production of the key branch point benzylisoquinoline alkaloid reticuline in yeast
    Article Snippet: Oligonucleotides were synthesized by either the Stanford Protein and Nucleic Acid Facility (Stanford, CA) or Integrated DNA Technologies (Coralville, IA). .. E. coli were cultured in Luria-Bertani media (EMD Chemicals) with appropriate antibiotic: 100 μg/mL ampicillin (EMD Chemicals) or 50 μg/mL kanamycin (EMD Chemicals).

    Article Title: Engineering strategies for the fermentative production of plant alkaloids in yeast
    Article Snippet: Oligonucleotides were synthesized by either the Stanford Protein and Nucleic Acid Facility (Stanford, CA) or Integrated DNA Technologies (Coralville, IA). .. E. coli were cultured in Luria-Bertani media (EMD Chemicals) with appropriate antibiotic: 100 µg/mL ampicillin (EMD Chemicals) or 50 µg/mL kanamycin (EMD Chemicals).

    Article Title: A synthetic library of RNA control modules for predictable tuning of gene expression in yeast
    Article Snippet: DNA synthesis was performed by Integrated DNA Technologies (Coralville, IA) or the Protein and Nucleic Acid Facility (Stanford, CA). .. Ligation products were electroporated into Escherichia coli DH10B (Invitrogen, Carlsbad, CA), and cells harboring cloned plasmids were maintained in Luria–Bertani media containing 50 mg/ml ampicillin (EMD Chemicals).

    Plasmid Preparation:

    Article Title: A high-throughput, quantitative cell-based screen for efficient tailoring of RNA device activity
    Article Snippet: Paragraph title: Plasmid and strain construction ... Ligation products were electroporated with a GenePulser XCell (Bio-Rad, Hercules, CA) into an Escherichia coli DH10B strain (Invitrogen, Carlsbad, CA), where cells harboring cloned plasmids were maintained in Luria-Bertani media containing 50 µg/mL ampicillin (EMD Chemicals, Philadelphia, PA).

    Article Title: Engineering ligand-responsive RNA controllers in yeast through the assembly of RNase III tuning modules
    Article Snippet: Paragraph title: Plasmid construction ... Ligation products were electroporated with a GenePulser XCell (Bio-Rad, Hercules, CA, USA) into Escherichia coli DH10B (Invitrogen, Carlsbad, CA), where cells harboring cloned plasmids were maintained in Luria-Bertani media containing 50 mg/ml ampicillin (EMD Chemicals).

    Article Title: De novo production of the key branch point benzylisoquinoline alkaloid reticuline in yeast
    Article Snippet: Paragraph title: 1.2.1 Yeast strain and plasmid construction ... E. coli were cultured in Luria-Bertani media (EMD Chemicals) with appropriate antibiotic: 100 μg/mL ampicillin (EMD Chemicals) or 50 μg/mL kanamycin (EMD Chemicals).

    Article Title: Crystallization and preliminary X-ray diffraction studies of a protein disulfide oxidoreductase from Aeropyrum pernix K1
    Article Snippet: This vector was then transformed into Escherichia coli strain RB791. .. Cells were grown to an OD600 of approximately 1 in Luria–Bertani media containing 0.1 mg ml−1 ampicillin (Sigma) at 310 K and the expression of Ap PDO was induced by 1 m M isopropyl-β- d -thiogalactoside (IPTG; Inalco).

    Article Title: Mechanical stability of αT-catenin and its activation by force for vinculin binding
    Article Snippet: Each of the resulting plasmids were cotransformed with a BirA plasmid ( ). .. Subsequently, the proteins of interest were expressed in Escherichia coli BL21 (DE3) cultured in Luria-Bertani media with D-Biotin (Sigma-Aldrich) and affinity purified using the His-tag.

    Article Title: Engineering strategies for the fermentative production of plant alkaloids in yeast
    Article Snippet: Paragraph title: 2.1 Plasmid and yeast strain construction ... E. coli were cultured in Luria-Bertani media (EMD Chemicals) with appropriate antibiotic: 100 µg/mL ampicillin (EMD Chemicals) or 50 µg/mL kanamycin (EMD Chemicals).

    Article Title: A synthetic library of RNA control modules for predictable tuning of gene expression in yeast
    Article Snippet: Paragraph title: Plasmid construction ... Ligation products were electroporated into Escherichia coli DH10B (Invitrogen, Carlsbad, CA), and cells harboring cloned plasmids were maintained in Luria–Bertani media containing 50 mg/ml ampicillin (EMD Chemicals).

    Nuclear Magnetic Resonance:

    Article Title: Millisecond Timescale Motions Connect Amino Acid Interaction Networks in Alpha Tryptophan Synthase
    Article Snippet: .. Overexpression and purification of αTS samples All samples were overexpressed using Escherichia coli BL21(DE3* ) cells grown in either Luria-Bertani media (EMD Millipore, Billerica, MA, USA) or M9 minimal media for NMR experiments. .. Samples for backbone relaxation dispersion were grown in 2 H2 O-based media with 15 N-labeled ammonium chloride (Cambridge Isotopes, Tewksbury, MA, USA).

    Article Title: Millisecond Timescale Motions Connect Amino Acid Interaction Networks in Alpha Tryptophan Synthase
    Article Snippet: .. All samples were overexpressed using Escherichia coli BL21(DE3* ) cells grown in either Luria-Bertani media (EMD Millipore, Billerica, MA, USA) or M9 minimal media for NMR experiments. .. Samples for backbone relaxation dispersion were grown in 2 H2 O-based media with 15 N-labeled ammonium chloride (Cambridge Isotopes, Tewksbury, MA, USA).

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  • 94
    Millipore luria bertani media
    Luria Bertani Media, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luria bertani media/product/Millipore
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    luria bertani media - by Bioz Stars, 2020-04
    94/100 stars
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