luria bertani lb medium  (Thermo Fisher)


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    Structured Review

    Thermo Fisher luria bertani lb medium
    Luria Bertani Lb Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luria bertani lb medium/product/Thermo Fisher
    Average 94 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    luria bertani lb medium - by Bioz Stars, 2020-04
    94/100 stars

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    Clone Assay:

    Article Title: Modulation of Enteropathogenic Escherichia coli Virulence by Quorum Sensing
    Article Snippet: Plasmid pVS241 was constructed by amplifying the qseA gene with Pfx polymerase (Invitrogen) by using primers qseAF and qseAR (Table ) and cloning this amplicon into pBADMycHisA (Invitrogen) digested with XhoI and HindIII. .. All E. coli strains were grown aerobically at 37°C in Luria-Bertani (LB) medium or Dulbecco's modified Eagle's medium (DMEM) (Invitrogen).

    Centrifugation:

    Article Title: Role and Mechanism of Action of C ? PvuII, a Regulatory Protein Conserved among Restriction-Modification Systems
    Article Snippet: To detect the active (C · Pvu IIFLAG ) and inactive (CLeu · Pvu IIFLAG ) fusion proteins analytically, cells were grown at 37°C in Luria-Bertani (LB) medium to mid-log phase and isopropyl-β- d -thiogalactopyranoside (IPTG; Life Technologies) was added to a final concentration of 1 mM to induce expression of C · Pvu IIFLAG . .. The cells were incubated a further 2 h and then sedimented by centrifugation at 5,000 × g for 10 min. Pellets were resuspended in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) sample buffer and analyzed on SDS–17.5% polyacrylamide gels.

    Amplification:

    Article Title: Modulation of Enteropathogenic Escherichia coli Virulence by Quorum Sensing
    Article Snippet: Plasmid pVS241 was constructed by amplifying the qseA gene with Pfx polymerase (Invitrogen) by using primers qseAF and qseAR (Table ) and cloning this amplicon into pBADMycHisA (Invitrogen) digested with XhoI and HindIII. .. All E. coli strains were grown aerobically at 37°C in Luria-Bertani (LB) medium or Dulbecco's modified Eagle's medium (DMEM) (Invitrogen).

    Acetylene Reduction Assay:

    Article Title: Oxygen-Insensitive Nitroreductases: Analysis of the Roles of nfsA and nfsB in Development of Resistance to 5-Nitrofuran Derivatives in Escherichia coli
    Article Snippet: AB1157 (F− thr-1 leu-6 thi-1 supE44 lacY1 galK2 ara-14 xyl-5 mtl-1 proA2 his-4 argE3 str-31 tsx-33 λ− sup-37 ) ( ) is the parent strain of the putative nitroreductase mutants NFR402, and NFR502 ( ). .. Bacterial strains were routinely grown at 37°C in Luria-Bertani (LB) medium or on LBA (LB medium with 1.5% agar), obtained as a premixed powder from Canadian Life Technologies Inc. (Burlington, Ontario, Canada).

    TA Cloning:

    Article Title: Oxygen-Insensitive Nitroreductases: Analysis of the Roles of nfsA and nfsB in Development of Resistance to 5-Nitrofuran Derivatives in Escherichia coli
    Article Snippet: The vectors used in this study were pUC118 , pKK232-8 from Pharmacia Biotech Inc. , and pCR 2.1 from the Original TA Cloning Kit. .. Bacterial strains were routinely grown at 37°C in Luria-Bertani (LB) medium or on LBA (LB medium with 1.5% agar), obtained as a premixed powder from Canadian Life Technologies Inc. (Burlington, Ontario, Canada).

    Construct:

    Article Title: Modulation of Enteropathogenic Escherichia coli Virulence by Quorum Sensing
    Article Snippet: Plasmid pVS241 was constructed by amplifying the qseA gene with Pfx polymerase (Invitrogen) by using primers qseAF and qseAR (Table ) and cloning this amplicon into pBADMycHisA (Invitrogen) digested with XhoI and HindIII. .. All E. coli strains were grown aerobically at 37°C in Luria-Bertani (LB) medium or Dulbecco's modified Eagle's medium (DMEM) (Invitrogen).

    Electrophoresis:

    Article Title: Role and Mechanism of Action of C ? PvuII, a Regulatory Protein Conserved among Restriction-Modification Systems
    Article Snippet: To detect the active (C · Pvu IIFLAG ) and inactive (CLeu · Pvu IIFLAG ) fusion proteins analytically, cells were grown at 37°C in Luria-Bertani (LB) medium to mid-log phase and isopropyl-β- d -thiogalactopyranoside (IPTG; Life Technologies) was added to a final concentration of 1 mM to induce expression of C · Pvu IIFLAG . .. After electrophoresis the samples were electroeluted (Bio-Rad Transblot electroeluter) onto nitrocellulose membranes for 1 h at 90 V. The primary and secondary antibodies for immunodetection were the mouse anti-FLAG.2 monoclonal antibody (Kodak) and a sheep antimouse antibody linked to horseradish peroxidase (Amersham).

    Incubation:

    Article Title: Isolation of a Bacterial Strain Able To Degrade Branched Nonylphenol
    Article Snippet: Liquid cultures were incubated on a shaker (40 to 50 rpm) in the dark. .. Luria-Bertani (LB) medium was composed of 10 g of tryptone, 5 g of yeast extract, and 10 g of sodium chloride per liter (Oxoid Ltd., Basingstoke, England; and VEL, Haasrode, Belgium) and used to culture the bacterial isolates, before they were stored at −70°C.

    Article Title: Role and Mechanism of Action of C ? PvuII, a Regulatory Protein Conserved among Restriction-Modification Systems
    Article Snippet: To detect the active (C · Pvu IIFLAG ) and inactive (CLeu · Pvu IIFLAG ) fusion proteins analytically, cells were grown at 37°C in Luria-Bertani (LB) medium to mid-log phase and isopropyl-β- d -thiogalactopyranoside (IPTG; Life Technologies) was added to a final concentration of 1 mM to induce expression of C · Pvu IIFLAG . .. The cells were incubated a further 2 h and then sedimented by centrifugation at 5,000 × g for 10 min. Pellets were resuspended in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) sample buffer and analyzed on SDS–17.5% polyacrylamide gels.

    Article Title: Characterization of a Glycosyl Transferase Inactivating Macrolides, Encoded by gimA from Streptomyces ambofaciens
    Article Snippet: Micrococcus luteus cells grown at 37°C in Luria-Bertani (LB) medium (Gibco BRL) were added to soft agar overlay on LB plates. .. Observation of growth inhibition zones was done after incubation of petri plates for 24 to 48 h at 37°C.

    Solubility:

    Article Title: Isolation of a Bacterial Strain Able To Degrade Branched Nonylphenol
    Article Snippet: Shaking of the mixture resulted in an emulsion of NP in the aqueous liquid agar (solubility of NP, ∼5 mg/liter). .. Luria-Bertani (LB) medium was composed of 10 g of tryptone, 5 g of yeast extract, and 10 g of sodium chloride per liter (Oxoid Ltd., Basingstoke, England; and VEL, Haasrode, Belgium) and used to culture the bacterial isolates, before they were stored at −70°C.

    Expressing:

    Article Title: Role and Mechanism of Action of C ? PvuII, a Regulatory Protein Conserved among Restriction-Modification Systems
    Article Snippet: .. To detect the active (C · Pvu IIFLAG ) and inactive (CLeu · Pvu IIFLAG ) fusion proteins analytically, cells were grown at 37°C in Luria-Bertani (LB) medium to mid-log phase and isopropyl-β- d -thiogalactopyranoside (IPTG; Life Technologies) was added to a final concentration of 1 mM to induce expression of C · Pvu IIFLAG . .. The cells were incubated a further 2 h and then sedimented by centrifugation at 5,000 × g for 10 min. Pellets were resuspended in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) sample buffer and analyzed on SDS–17.5% polyacrylamide gels.

    Modification:

    Article Title: Influence of environmental factors in the adherence of an atypical enteropathogenic Escherichia coli strain to epithelial cells
    Article Snippet: .. Bacterial strains were routinely grown aerobically in Luria-Bertani (LB) medium or Dulbecco’s modified Eagle’s medium (DMEM, Gibco, USA) at 37°C. .. When appropriate, strains were cultured in the presence of nalidixic acid (20 μg/ml) and/or zeocin (60 μg/ml).

    Article Title: Modulation of Enteropathogenic Escherichia coli Virulence by Quorum Sensing
    Article Snippet: .. All E. coli strains were grown aerobically at 37°C in Luria-Bertani (LB) medium or Dulbecco's modified Eagle's medium (DMEM) (Invitrogen). ..

    Derivative Assay:

    Article Title: Oxygen-Insensitive Nitroreductases: Analysis of the Roles of nfsA and nfsB in Development of Resistance to 5-Nitrofuran Derivatives in Escherichia coli
    Article Snippet: Strain SIL41 was derived from a cross between AB1157 and an HfrH strain lacking NfsA and NfsB activities ( ). .. Bacterial strains were routinely grown at 37°C in Luria-Bertani (LB) medium or on LBA (LB medium with 1.5% agar), obtained as a premixed powder from Canadian Life Technologies Inc. (Burlington, Ontario, Canada).

    Conjugation Assay:

    Article Title: Characterization of a Glycosyl Transferase Inactivating Macrolides, Encoded by gimA from Streptomyces ambofaciens
    Article Snippet: AS1 medium ( ) was used for conjugation experiments, and cultures were grown at 37°C (see below). .. Micrococcus luteus cells grown at 37°C in Luria-Bertani (LB) medium (Gibco BRL) were added to soft agar overlay on LB plates.

    Transferring:

    Article Title: Isolation of a Bacterial Strain Able To Degrade Branched Nonylphenol
    Article Snippet: Approximately 1.5 g of NP per liter was added with a sterile Pasteur pipette. .. Luria-Bertani (LB) medium was composed of 10 g of tryptone, 5 g of yeast extract, and 10 g of sodium chloride per liter (Oxoid Ltd., Basingstoke, England; and VEL, Haasrode, Belgium) and used to culture the bacterial isolates, before they were stored at −70°C.

    Infection:

    Article Title: Deletion of TnAbaR23 Results in both Expected and Unexpected Antibiogram Changes in a Multidrug-Resistant Acinetobacter baumannii Strain
    Article Snippet: A. baumannii strain A424, provided by Kevin Towner, Queen's Medical Centre, Nottingham, United Kingdom, was originally isolated from a patient in Croatia with an invasive infection. .. All strains were grown at 37°C at 200 rpm in Luria-Bertani (LB) medium, except for selection of A. baumannii merodiploids which was performed on Simmons citrate agar (Oxoid, Basingstoke, United Kingdom) ( ).

    Generated:

    Article Title: Modulation of Enteropathogenic Escherichia coli Virulence by Quorum Sensing
    Article Snippet: The EPEC luxS mutant strain, named VS102, was generated by allelic exchange of the luxS gene in pVS98 by using chloramphenicol and sucrose selection as previously described ( ). .. All E. coli strains were grown aerobically at 37°C in Luria-Bertani (LB) medium or Dulbecco's modified Eagle's medium (DMEM) (Invitrogen).

    Inhibition:

    Article Title: Characterization of a Glycosyl Transferase Inactivating Macrolides, Encoded by gimA from Streptomyces ambofaciens
    Article Snippet: Micrococcus luteus cells grown at 37°C in Luria-Bertani (LB) medium (Gibco BRL) were added to soft agar overlay on LB plates. .. Observation of growth inhibition zones was done after incubation of petri plates for 24 to 48 h at 37°C.

    Polymerase Chain Reaction:

    Article Title: Oxygen-Insensitive Nitroreductases: Analysis of the Roles of nfsA and nfsB in Development of Resistance to 5-Nitrofuran Derivatives in Escherichia coli
    Article Snippet: The vectors used in this study were pUC118 , pKK232-8 from Pharmacia Biotech Inc. , and pCR 2.1 from the Original TA Cloning Kit. .. Bacterial strains were routinely grown at 37°C in Luria-Bertani (LB) medium or on LBA (LB medium with 1.5% agar), obtained as a premixed powder from Canadian Life Technologies Inc. (Burlington, Ontario, Canada).

    Sucrose Selection:

    Article Title: Modulation of Enteropathogenic Escherichia coli Virulence by Quorum Sensing
    Article Snippet: The EPEC luxS mutant strain, named VS102, was generated by allelic exchange of the luxS gene in pVS98 by using chloramphenicol and sucrose selection as previously described ( ). .. All E. coli strains were grown aerobically at 37°C in Luria-Bertani (LB) medium or Dulbecco's modified Eagle's medium (DMEM) (Invitrogen).

    Nucleic Acid Electrophoresis:

    Article Title: Role and Mechanism of Action of C ? PvuII, a Regulatory Protein Conserved among Restriction-Modification Systems
    Article Snippet: To detect the active (C · Pvu IIFLAG ) and inactive (CLeu · Pvu IIFLAG ) fusion proteins analytically, cells were grown at 37°C in Luria-Bertani (LB) medium to mid-log phase and isopropyl-β- d -thiogalactopyranoside (IPTG; Life Technologies) was added to a final concentration of 1 mM to induce expression of C · Pvu IIFLAG . .. The cells were incubated a further 2 h and then sedimented by centrifugation at 5,000 × g for 10 min. Pellets were resuspended in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) sample buffer and analyzed on SDS–17.5% polyacrylamide gels.

    Mutagenesis:

    Article Title: Influence of environmental factors in the adherence of an atypical enteropathogenic Escherichia coli strain to epithelial cells
    Article Snippet: The fimA mutant and complemented strains were obtained in a subsequent study from our laboratory [ ]. .. Bacterial strains were routinely grown aerobically in Luria-Bertani (LB) medium or Dulbecco’s modified Eagle’s medium (DMEM, Gibco, USA) at 37°C.

    Article Title: Modulation of Enteropathogenic Escherichia coli Virulence by Quorum Sensing
    Article Snippet: The qseA mutant strain VS193 has been previously described ( ). .. All E. coli strains were grown aerobically at 37°C in Luria-Bertani (LB) medium or Dulbecco's modified Eagle's medium (DMEM) (Invitrogen).

    Article Title: Salmonella enterica Serovar Typhimurium Alters the Extracellular Proteome of Macrophages and Leads to the Production of Proinflammatory Exosomes
    Article Snippet: .. Typhimurium wild-type ATCC 14028 (a gift from David Holden [ ]), wild-type UK-1 (χ3761; a gift from Roy Curtiss III), and mutant UK-1 (Δ pagL7 Δ pagP81 ::Plpp lpxE Δ lpxR9 UK-1; χ9705, produces 1-monophosphoryl lipid A, in the background of χ3761; a generous gift from Roy Curtiss III [ ]) were cultured in Luria-Bertani (LB) medium at 37°C and 200 rpm (MaxQ 6000 incubator; Thermo Scientific, USA) for 18 h. Overnight cultures were split to achieve an optical density at 600 nm (OD600 ) of 0.05 and were cultured until early exponential phase (OD600 = 0.5). .. Cells were washed with PBS and incubated in growth medium containing no FBS or antibiotics for 90 min before infection.

    Article Title: Characterization of a Glycosyl Transferase Inactivating Macrolides, Encoded by gimA from Streptomyces ambofaciens
    Article Snippet: Micrococcus luteus cells grown at 37°C in Luria-Bertani (LB) medium (Gibco BRL) were added to soft agar overlay on LB plates. .. The M. luteus congocidin (CG)-resistant mutant was grown in LB medium in the presence of CG at 5 μg/ml.

    Isolation:

    Article Title: Influence of environmental factors in the adherence of an atypical enteropathogenic Escherichia coli strain to epithelial cells
    Article Snippet: Bacterial strain The aEPEC 1551–2 strain (serotype ONT:H− ) was isolated as part of standard patient care from a diarrheic child (23 months old), in the absence of other recognized pathogens, during an epidemiological study of diarrhea carried out at the Universidade Federal de São Paulo (UNIFESP), Brazil. .. Bacterial strains were routinely grown aerobically in Luria-Bertani (LB) medium or Dulbecco’s modified Eagle’s medium (DMEM, Gibco, USA) at 37°C.

    Article Title: Swine-Derived Probiotic Lactobacillus plantarum Inhibits Growth and Adhesion of Enterotoxigenic Escherichia coli and Mediates Host Defense
    Article Snippet: Lactobacillus plantarum ZLP001 was isolated from a healthy piglet in our laboratory, identified by the China Center of Industrial Culture Collection (Beijing, China), and preserved in the China General Microbiological Culture Collection Center (CGMCC No. 7370). .. F4+ ETEC were grown in Luria-Bertani (LB) medium (Oxoid, Basingstoke, United Kingdom) at 37°C.

    Article Title: Deletion of TnAbaR23 Results in both Expected and Unexpected Antibiogram Changes in a Multidrug-Resistant Acinetobacter baumannii Strain
    Article Snippet: A. baumannii strain A424, provided by Kevin Towner, Queen's Medical Centre, Nottingham, United Kingdom, was originally isolated from a patient in Croatia with an invasive infection. .. All strains were grown at 37°C at 200 rpm in Luria-Bertani (LB) medium, except for selection of A. baumannii merodiploids which was performed on Simmons citrate agar (Oxoid, Basingstoke, United Kingdom) ( ).

    Immunodetection:

    Article Title: Role and Mechanism of Action of C ? PvuII, a Regulatory Protein Conserved among Restriction-Modification Systems
    Article Snippet: To detect the active (C · Pvu IIFLAG ) and inactive (CLeu · Pvu IIFLAG ) fusion proteins analytically, cells were grown at 37°C in Luria-Bertani (LB) medium to mid-log phase and isopropyl-β- d -thiogalactopyranoside (IPTG; Life Technologies) was added to a final concentration of 1 mM to induce expression of C · Pvu IIFLAG . .. After electrophoresis the samples were electroeluted (Bio-Rad Transblot electroeluter) onto nitrocellulose membranes for 1 h at 90 V. The primary and secondary antibodies for immunodetection were the mouse anti-FLAG.2 monoclonal antibody (Kodak) and a sheep antimouse antibody linked to horseradish peroxidase (Amersham).

    Labeling:

    Article Title: Salmonella enterica Serovar Typhimurium Alters the Extracellular Proteome of Macrophages and Leads to the Production of Proinflammatory Exosomes
    Article Snippet: Cells were stained with fluorochrome-conjugated anti-mouse immunoglobulin antibodies, including labeled mouse CD11b, CD11c, and MHC-II, and analyzed using a BD LSR Fortessa cell analyzer (BD Biosciences, USA). .. Typhimurium wild-type ATCC 14028 (a gift from David Holden [ ]), wild-type UK-1 (χ3761; a gift from Roy Curtiss III), and mutant UK-1 (Δ pagL7 Δ pagP81 ::Plpp lpxE Δ lpxR9 UK-1; χ9705, produces 1-monophosphoryl lipid A, in the background of χ3761; a generous gift from Roy Curtiss III [ ]) were cultured in Luria-Bertani (LB) medium at 37°C and 200 rpm (MaxQ 6000 incubator; Thermo Scientific, USA) for 18 h. Overnight cultures were split to achieve an optical density at 600 nm (OD600 ) of 0.05 and were cultured until early exponential phase (OD600 = 0.5).

    Mouse Assay:

    Article Title: Salmonella enterica Serovar Typhimurium Alters the Extracellular Proteome of Macrophages and Leads to the Production of Proinflammatory Exosomes
    Article Snippet: Bone marrow cells were harvested from the femurs and tibias of BALB/c mice to generate BMDMs and BMDCs. .. Typhimurium wild-type ATCC 14028 (a gift from David Holden [ ]), wild-type UK-1 (χ3761; a gift from Roy Curtiss III), and mutant UK-1 (Δ pagL7 Δ pagP81 ::Plpp lpxE Δ lpxR9 UK-1; χ9705, produces 1-monophosphoryl lipid A, in the background of χ3761; a generous gift from Roy Curtiss III [ ]) were cultured in Luria-Bertani (LB) medium at 37°C and 200 rpm (MaxQ 6000 incubator; Thermo Scientific, USA) for 18 h. Overnight cultures were split to achieve an optical density at 600 nm (OD600 ) of 0.05 and were cultured until early exponential phase (OD600 = 0.5).

    Cell Culture:

    Article Title: Influence of environmental factors in the adherence of an atypical enteropathogenic Escherichia coli strain to epithelial cells
    Article Snippet: Bacterial strains were routinely grown aerobically in Luria-Bertani (LB) medium or Dulbecco’s modified Eagle’s medium (DMEM, Gibco, USA) at 37°C. .. When appropriate, strains were cultured in the presence of nalidixic acid (20 μg/ml) and/or zeocin (60 μg/ml).

    Article Title: Salmonella enterica Serovar Typhimurium Alters the Extracellular Proteome of Macrophages and Leads to the Production of Proinflammatory Exosomes
    Article Snippet: .. Typhimurium wild-type ATCC 14028 (a gift from David Holden [ ]), wild-type UK-1 (χ3761; a gift from Roy Curtiss III), and mutant UK-1 (Δ pagL7 Δ pagP81 ::Plpp lpxE Δ lpxR9 UK-1; χ9705, produces 1-monophosphoryl lipid A, in the background of χ3761; a generous gift from Roy Curtiss III [ ]) were cultured in Luria-Bertani (LB) medium at 37°C and 200 rpm (MaxQ 6000 incubator; Thermo Scientific, USA) for 18 h. Overnight cultures were split to achieve an optical density at 600 nm (OD600 ) of 0.05 and were cultured until early exponential phase (OD600 = 0.5). .. Cells were washed with PBS and incubated in growth medium containing no FBS or antibiotics for 90 min before infection.

    SDS Page:

    Article Title: Role and Mechanism of Action of C ? PvuII, a Regulatory Protein Conserved among Restriction-Modification Systems
    Article Snippet: To detect the active (C · Pvu IIFLAG ) and inactive (CLeu · Pvu IIFLAG ) fusion proteins analytically, cells were grown at 37°C in Luria-Bertani (LB) medium to mid-log phase and isopropyl-β- d -thiogalactopyranoside (IPTG; Life Technologies) was added to a final concentration of 1 mM to induce expression of C · Pvu IIFLAG . .. The cells were incubated a further 2 h and then sedimented by centrifugation at 5,000 × g for 10 min. Pellets were resuspended in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) sample buffer and analyzed on SDS–17.5% polyacrylamide gels.

    Plasmid Preparation:

    Article Title: Modulation of Enteropathogenic Escherichia coli Virulence by Quorum Sensing
    Article Snippet: Plasmid pVS241 was constructed by amplifying the qseA gene with Pfx polymerase (Invitrogen) by using primers qseAF and qseAR (Table ) and cloning this amplicon into pBADMycHisA (Invitrogen) digested with XhoI and HindIII. .. All E. coli strains were grown aerobically at 37°C in Luria-Bertani (LB) medium or Dulbecco's modified Eagle's medium (DMEM) (Invitrogen).

    Article Title: The Staphylococcus aureus KdpDE Two-Component System Couples Extracellular K+ Sensing and Agr Signaling to Infection Programming ▿ Sensing and Agr Signaling to Infection Programming ▿ † Sensing and Agr Signaling to Infection Programming ▿ † ‡
    Article Snippet: .. Staphylococcus aureus and E. coli were grown in Luria-Bertani (LB) medium or tryptic soy broth (TSB; soybean-casein digest medium USP; Oxoid) medium with the appropriate antibiotics for plasmid selection and maintenance. ..

    Article Title: Oxygen-Insensitive Nitroreductases: Analysis of the Roles of nfsA and nfsB in Development of Resistance to 5-Nitrofuran Derivatives in Escherichia coli
    Article Snippet: DH5α [ supE44 lacU169 (φ80 lacZ ΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 ] is a standard laboratory strain used for most plasmid manipulations. .. Bacterial strains were routinely grown at 37°C in Luria-Bertani (LB) medium or on LBA (LB medium with 1.5% agar), obtained as a premixed powder from Canadian Life Technologies Inc. (Burlington, Ontario, Canada).

    Selection:

    Article Title: Deletion of TnAbaR23 Results in both Expected and Unexpected Antibiogram Changes in a Multidrug-Resistant Acinetobacter baumannii Strain
    Article Snippet: .. All strains were grown at 37°C at 200 rpm in Luria-Bertani (LB) medium, except for selection of A. baumannii merodiploids which was performed on Simmons citrate agar (Oxoid, Basingstoke, United Kingdom) ( ). ..

    Article Title: The Staphylococcus aureus KdpDE Two-Component System Couples Extracellular K+ Sensing and Agr Signaling to Infection Programming ▿ Sensing and Agr Signaling to Infection Programming ▿ † Sensing and Agr Signaling to Infection Programming ▿ † ‡
    Article Snippet: .. Staphylococcus aureus and E. coli were grown in Luria-Bertani (LB) medium or tryptic soy broth (TSB; soybean-casein digest medium USP; Oxoid) medium with the appropriate antibiotics for plasmid selection and maintenance. ..

    Concentration Assay:

    Article Title: Role and Mechanism of Action of C ? PvuII, a Regulatory Protein Conserved among Restriction-Modification Systems
    Article Snippet: .. To detect the active (C · Pvu IIFLAG ) and inactive (CLeu · Pvu IIFLAG ) fusion proteins analytically, cells were grown at 37°C in Luria-Bertani (LB) medium to mid-log phase and isopropyl-β- d -thiogalactopyranoside (IPTG; Life Technologies) was added to a final concentration of 1 mM to induce expression of C · Pvu IIFLAG . .. The cells were incubated a further 2 h and then sedimented by centrifugation at 5,000 × g for 10 min. Pellets were resuspended in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) sample buffer and analyzed on SDS–17.5% polyacrylamide gels.

    Article Title: pAM401-Based Shuttle Vectors That Enable Overexpression of Promoterless Genes and One-Step Purification of Tag Fusion Proteins Directly from Enterococcus faecalis
    Article Snippet: E. coli strains were grown in Luria-Bertani (LB) medium (GIBCO BRL, Life Technologies, Grand Island, N.Y.) unless otherwise stated. .. X-Gal (5-bromo-4-chloro-3-indolyl-β- d -galactopyranoside) (Wako Pure Chemical Industries, Ltd., Osaka, Japan) was used at a concentration of 200 μg/ml to obtain blue color development on agar plates.

    Staining:

    Article Title: Salmonella enterica Serovar Typhimurium Alters the Extracellular Proteome of Macrophages and Leads to the Production of Proinflammatory Exosomes
    Article Snippet: Cells were stained with fluorochrome-conjugated anti-mouse immunoglobulin antibodies, including labeled mouse CD11b, CD11c, and MHC-II, and analyzed using a BD LSR Fortessa cell analyzer (BD Biosciences, USA). .. Typhimurium wild-type ATCC 14028 (a gift from David Holden [ ]), wild-type UK-1 (χ3761; a gift from Roy Curtiss III), and mutant UK-1 (Δ pagL7 Δ pagP81 ::Plpp lpxE Δ lpxR9 UK-1; χ9705, produces 1-monophosphoryl lipid A, in the background of χ3761; a generous gift from Roy Curtiss III [ ]) were cultured in Luria-Bertani (LB) medium at 37°C and 200 rpm (MaxQ 6000 incubator; Thermo Scientific, USA) for 18 h. Overnight cultures were split to achieve an optical density at 600 nm (OD600 ) of 0.05 and were cultured until early exponential phase (OD600 = 0.5).

    other:

    Article Title: The Escherichia coli Efflux Pump TolC Promotes Aggregation of Enteroaggregative E. coli 042 ▿
    Article Snippet: All E. coli strains were grown aerobically at 37°C in Luria-Bertani (LB) medium or Dulbecco's minimal essential medium with 0.45% glucose (high-glucose DMEM) (Invitrogen, Carlsbad, CA).

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  • 99
    Thermo Fisher luria bertani medium
    Detection of Q in tRNA Asp GUC as a representative of the salvage of the Q precursors preQ 0 and preQ 1 . E. coli bulk tRNAs were separated in an 8 M urea, 8% polyacrylamide gel containing 0.5% 3-(acrylamido)phenylboronic acid and then transferred to a nylon membrane. The transferred tRNAs were probed with a biotinylated primer, and visualized by chemiluminescence. ( A ) tRNAs modified with Q migrate slower than unmodified tRNA, as illustrated with tRNA from Wild Type (WT), and Δ tgt grown in <t>Luria-Bertani</t> (LB - positive and negative control, respectively). tRNAs from Δ queD and Δ queD Δ yhhQ grown in defined minimal medium M9 + 0.5% glycerol do not have Q; ( B ) test of the salvage capability of the WT (positive control for Q detection), Δ queD and Δ queD Δ yhhQ strains towards mock (negative control), 10 nM preQ 0 and 10 nM preQ 1 treatments. Representative Northern blots shown.
    Luria Bertani Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luria bertani medium/product/Thermo Fisher
    Average 99 stars, based on 41 article reviews
    Price from $9.99 to $1999.99
    luria bertani medium - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    94
    Thermo Fisher luria bertani lb liquid medium
    Detection of Q in tRNA Asp GUC as a representative of the salvage of the Q precursors preQ 0 and preQ 1 . E. coli bulk tRNAs were separated in an 8 M urea, 8% polyacrylamide gel containing 0.5% 3-(acrylamido)phenylboronic acid and then transferred to a nylon membrane. The transferred tRNAs were probed with a biotinylated primer, and visualized by chemiluminescence. ( A ) tRNAs modified with Q migrate slower than unmodified tRNA, as illustrated with tRNA from Wild Type (WT), and Δ tgt grown in <t>Luria-Bertani</t> (LB - positive and negative control, respectively). tRNAs from Δ queD and Δ queD Δ yhhQ grown in defined minimal medium M9 + 0.5% glycerol do not have Q; ( B ) test of the salvage capability of the WT (positive control for Q detection), Δ queD and Δ queD Δ yhhQ strains towards mock (negative control), 10 nM preQ 0 and 10 nM preQ 1 treatments. Representative Northern blots shown.
    Luria Bertani Lb Liquid Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luria bertani lb liquid medium/product/Thermo Fisher
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    luria bertani lb liquid medium - by Bioz Stars, 2020-04
    94/100 stars
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    Detection of Q in tRNA Asp GUC as a representative of the salvage of the Q precursors preQ 0 and preQ 1 . E. coli bulk tRNAs were separated in an 8 M urea, 8% polyacrylamide gel containing 0.5% 3-(acrylamido)phenylboronic acid and then transferred to a nylon membrane. The transferred tRNAs were probed with a biotinylated primer, and visualized by chemiluminescence. ( A ) tRNAs modified with Q migrate slower than unmodified tRNA, as illustrated with tRNA from Wild Type (WT), and Δ tgt grown in Luria-Bertani (LB - positive and negative control, respectively). tRNAs from Δ queD and Δ queD Δ yhhQ grown in defined minimal medium M9 + 0.5% glycerol do not have Q; ( B ) test of the salvage capability of the WT (positive control for Q detection), Δ queD and Δ queD Δ yhhQ strains towards mock (negative control), 10 nM preQ 0 and 10 nM preQ 1 treatments. Representative Northern blots shown.

    Journal: Biomolecules

    Article Title: The Escherichia coli COG1738 Member YhhQ Is Involved in 7-Cyanodeazaguanine (preQ0) Transport

    doi: 10.3390/biom7010012

    Figure Lengend Snippet: Detection of Q in tRNA Asp GUC as a representative of the salvage of the Q precursors preQ 0 and preQ 1 . E. coli bulk tRNAs were separated in an 8 M urea, 8% polyacrylamide gel containing 0.5% 3-(acrylamido)phenylboronic acid and then transferred to a nylon membrane. The transferred tRNAs were probed with a biotinylated primer, and visualized by chemiluminescence. ( A ) tRNAs modified with Q migrate slower than unmodified tRNA, as illustrated with tRNA from Wild Type (WT), and Δ tgt grown in Luria-Bertani (LB - positive and negative control, respectively). tRNAs from Δ queD and Δ queD Δ yhhQ grown in defined minimal medium M9 + 0.5% glycerol do not have Q; ( B ) test of the salvage capability of the WT (positive control for Q detection), Δ queD and Δ queD Δ yhhQ strains towards mock (negative control), 10 nM preQ 0 and 10 nM preQ 1 treatments. Representative Northern blots shown.

    Article Snippet: Strains and Growth Conditions For standard procedures, E. coli strains were grown in Luria–Bertani medium (LB - Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C.

    Techniques: Modification, Negative Control, Positive Control, Northern Blot