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Difco luria bertani lb medium
Luria Bertani Lb Medium, supplied by Difco, used in various techniques. Bioz Stars score: 94/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Amplification:

Article Title: Biomimetic strategy for constructing Clostridium thermocellum cellulosomal operons in Bacillus subtilis
Article Snippet: All the bacterial strains used were cultured in Luria–Bertani (LB) medium (Difco Laboratories, Detroit, MI) supplemented with ampicillin 50 µg/ml (for E. coli ) or tetracycline 10 µg/ml (for B. subtilis ) and incubated at 37 °C for 24 h. The restriction enzymes were purchased from New England Biolabs and all the chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise stated. .. The cellulosomal genes were amplified from C. thermocellum ATCC 27405 genomic DNA using gene-specific primers (Additional file : Table S2).

Binding Assay:

Article Title: Structural Basis for the Inhibition of the Chromatin Repressor BAHD1 by the Bacterial Nucleomodulin LntA
Article Snippet: A 72-nucleotide insertion upstream of the ribosome binding site (RBS; highlighted in gray) provides an additional putative σB binding site. .. L. monocytogenes and E. coli strains were grown in brain heart infusion (BHI) and Luria-Bertani (LB) medium (Difco), respectively, at 37°C.

Synthesized:

Article Title: A Nuclease from Streptococcus mutans Facilitates Biofilm Dispersal and Escape from Killing by Neutrophil Extracellular Traps
Article Snippet: Escherichia coli strains were grown in Luria–Bertani (LB) medium (Difco, Sparks, MD, USA). .. The competence stimulating peptide (CSP) was synthesized by Life Invitrogen (Shanghai, China).

Mutagenesis:

Article Title: Important Role of Class I Heat Shock Genes hrcA and dnaK in the Heat Shock Response and the Response to pH and NaCl Stress of Group I Clostridium botulinum Strain ATCC 3502 ▿
Article Snippet: The C. botulinum ATCC 3502 parent strain was stored as a spore stock culture at 4°C; the mutant strains were stored as frozen stock cultures at −70°C in tryptone-peptone-glucose-yeast extract (TPGY) broth (50 g/liter tryptone, 5 g/liter peptone, 20 g/liter yeast extract [Difco, BD Diagnostic Systems, Sparks, MD], 4 g/liter glucose [VWR International, Leuven, Belgium], 1 g/liter sodium thioglycolate [Merck, Darmstadt, Germany]) supplemented with 20% glycerol. .. Escherichia coli was grown in Luria-Bertani (LB) medium (Difco) or on 2× TY agar plates (16 g/liter tryptone, 10 g/liter yeast extract, 5 g/liter NaCl [J. T. Baker, Deventer, Netherlands]) at 37°C under aerobic conditions.

Article Title: Biomimetic strategy for constructing Clostridium thermocellum cellulosomal operons in Bacillus subtilis
Article Snippet: Restriction modification-deficient mutant strain B. subtilis RM125 and protease-mutant B. subtilis WB800 were used as hosts for expressing various cellulosomal complexes [ ]. .. All the bacterial strains used were cultured in Luria–Bertani (LB) medium (Difco Laboratories, Detroit, MI) supplemented with ampicillin 50 µg/ml (for E. coli ) or tetracycline 10 µg/ml (for B. subtilis ) and incubated at 37 °C for 24 h. The restriction enzymes were purchased from New England Biolabs and all the chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise stated.

Cell Culture:

Article Title: Biomimetic strategy for constructing Clostridium thermocellum cellulosomal operons in Bacillus subtilis
Article Snippet: .. All the bacterial strains used were cultured in Luria–Bertani (LB) medium (Difco Laboratories, Detroit, MI) supplemented with ampicillin 50 µg/ml (for E. coli ) or tetracycline 10 µg/ml (for B. subtilis ) and incubated at 37 °C for 24 h. The restriction enzymes were purchased from New England Biolabs and all the chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise stated. ..

Sequencing:

Article Title: Structural Basis for the Inhibition of the Chromatin Repressor BAHD1 by the Bacterial Nucleomodulin LntA
Article Snippet: In contrast, the consensus PrfA box (highlighted in purple in the EGD-e sequence) is degenerated in FSL S4-120. .. L. monocytogenes and E. coli strains were grown in brain heart infusion (BHI) and Luria-Bertani (LB) medium (Difco), respectively, at 37°C.

Incubation:

Article Title: Biomimetic strategy for constructing Clostridium thermocellum cellulosomal operons in Bacillus subtilis
Article Snippet: .. All the bacterial strains used were cultured in Luria–Bertani (LB) medium (Difco Laboratories, Detroit, MI) supplemented with ampicillin 50 µg/ml (for E. coli ) or tetracycline 10 µg/ml (for B. subtilis ) and incubated at 37 °C for 24 h. The restriction enzymes were purchased from New England Biolabs and all the chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise stated. ..

Article Title: Burkholderia cenocepacia and Salmonella enterica ArnT proteins that transfer 4-amino-4-deoxy-l-arabinose to lipopolysaccharide share membrane topology and functional amino acids
Article Snippet: Bacteria were grown aerobically at 37 °C in Luria-Bertani (LB) medium (Difco). .. For recombinant protein expression, bacteria were grown overnight in 5 mL of LB, diluted to A 600 of 0.1, and incubated at 37 °C for 2 h until cultures reached A 600 of 0.5, at which point l -arabinose was added and cells incubated for 3 h at 37 °C.

other:

Article Title: Antifungal amphiphilic aminoglycoside K20: bioactivities and mechanism of action
Article Snippet: Bacterial strains were grown at 37°C for 24 h on Luria-Bertani (LB) medium (Sambrook et al., ) except for S. aureus ATCC6538 which was grown on Mueller-Hinton medium (Difco, BD, Franklin Lakes, NJ, USA).

Article Title: Efflux-mediated resistance to a benzothiadiazol derivative effective against Burkholderia cenocepacia
Article Snippet: Bacterial Strains and Growth Conditions Burkholderia cenocepacia strains and B. cepacia complex (Bcc) species were grown in Luria-Bertani (LB) medium (Difco), with shaking at 200 rpm, or on LB agar, at 37°C.

Article Title: Analysis of a Heme-Dependent Signal Transduction System in Corynebacterium diphtheriae: Deletion of the chrAS Genes Results in Heme Sensitivity and Diminished Heme-Dependent Activation of the hmuO Promoter
Article Snippet: Luria-Bertani (LB) medium was used for culturing of E. coli , and heart infusion broth (Difco, Detroit, MI) containing 0.2% Tween 80 (HIBTW) was used for growth of C. diphtheriae strains.

Article Title: Protection from Oxidative Stress Relies Mainly on Derepression of OxyR-Dependent KatB and Dps in Shewanella oneidensis
Article Snippet: Unless otherwise noted, E. coli and S. oneidensis strains under aerobic conditions were grown in Luria-Bertani (LB) medium (Difco, Detroit, MI) at 37°C and 30°C, respectively.

Article Title: Pacbio sequencing of copper-tolerant Xanthomonas citri reveals presence of a chimeric plasmid structure and provides insights into reassortment and shuffling of transcription activator-like effectors among X. citri strains
Article Snippet: Xc-03-1638-1-1 and Escherichia coli (Ec) strains were maintained on Luria-Bertani (LB) medium [ ], and also stored in nutrient broth (Difco™) containing 30% glycerol, in a − 80 °C freezer.

Article Title: Characterization of a Stress-Induced Alternate Sigma Factor, RpoS, of Coxiella burnetii and Its Expression during the Development Cycle
Article Snippet: Luria-Bertani (LB) medium was purchased from Difco Laboratories (Detroit, Mich.), and M9 minimal medium was prepared according to a laboratory manual ( ).

Expressing:

Article Title: Structural Basis for the Inhibition of the Chromatin Repressor BAHD1 by the Bacterial Nucleomodulin LntA
Article Snippet: L. monocytogenes and E. coli strains were grown in brain heart infusion (BHI) and Luria-Bertani (LB) medium (Difco), respectively, at 37°C. .. *, except for pP1 vectors designed for expression in Listeria , the lntA insert corresponds to the sequence encoding a mature form of the protein, devoid of its signal peptide (residues 1 to 33), which is only of use for secretion by Listeria monocytogenes ; †, the sequence of lntA hu has optimized codon usage for expression in human cells.

Article Title: Biomimetic strategy for constructing Clostridium thermocellum cellulosomal operons in Bacillus subtilis
Article Snippet: Restriction modification-deficient mutant strain B. subtilis RM125 and protease-mutant B. subtilis WB800 were used as hosts for expressing various cellulosomal complexes [ ]. .. All the bacterial strains used were cultured in Luria–Bertani (LB) medium (Difco Laboratories, Detroit, MI) supplemented with ampicillin 50 µg/ml (for E. coli ) or tetracycline 10 µg/ml (for B. subtilis ) and incubated at 37 °C for 24 h. The restriction enzymes were purchased from New England Biolabs and all the chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise stated.

Article Title: Burkholderia cenocepacia and Salmonella enterica ArnT proteins that transfer 4-amino-4-deoxy-l-arabinose to lipopolysaccharide share membrane topology and functional amino acids
Article Snippet: Bacteria were grown aerobically at 37 °C in Luria-Bertani (LB) medium (Difco). .. For recombinant protein expression, bacteria were grown overnight in 5 mL of LB, diluted to A 600 of 0.1, and incubated at 37 °C for 2 h until cultures reached A 600 of 0.5, at which point l -arabinose was added and cells incubated for 3 h at 37 °C.

Modification:

Article Title: Biomimetic strategy for constructing Clostridium thermocellum cellulosomal operons in Bacillus subtilis
Article Snippet: Restriction modification-deficient mutant strain B. subtilis RM125 and protease-mutant B. subtilis WB800 were used as hosts for expressing various cellulosomal complexes [ ]. .. All the bacterial strains used were cultured in Luria–Bertani (LB) medium (Difco Laboratories, Detroit, MI) supplemented with ampicillin 50 µg/ml (for E. coli ) or tetracycline 10 µg/ml (for B. subtilis ) and incubated at 37 °C for 24 h. The restriction enzymes were purchased from New England Biolabs and all the chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise stated.

Recombinant:

Article Title: Burkholderia cenocepacia and Salmonella enterica ArnT proteins that transfer 4-amino-4-deoxy-l-arabinose to lipopolysaccharide share membrane topology and functional amino acids
Article Snippet: Bacteria were grown aerobically at 37 °C in Luria-Bertani (LB) medium (Difco). .. For recombinant protein expression, bacteria were grown overnight in 5 mL of LB, diluted to A 600 of 0.1, and incubated at 37 °C for 2 h until cultures reached A 600 of 0.5, at which point l -arabinose was added and cells incubated for 3 h at 37 °C.

Cellular Antioxidant Activity Assay:

Article Title: Blocking Phosphatidylcholine Utilization in Pseudomonas aeruginosa, via Mutagenesis of Fatty Acid, Glycerol and Choline Degradation Pathways, Confirms the Importance of This Nutrient Source In Vivo
Article Snippet: .. Briefly, seven growth media with different carbon sources, including Luria-Bertani (LB) medium, casamino acids (CAA), glucose, PC, C18∶1 Δ9 , choline, and glycerol, were used in this study. .. The bacterial CFU were determined after inoculation into each of the medium for 24–48 h. The CI was calculated as the CFU ratio of mutant/wildtype recovered at each time point divided by the CFU ratio of mutant/wildtype in the input inoculum .

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    94
    Difco nacl
    Titration of 2,2’-dipyridyl concentration. A. <t>salmonicida</t> strain LFI1238 was grown in LB containing 1% <t>NaCl</t> to an optical density at 0.4 (600 nm). The culture was split into six individual flasks and supplemented with different concentrations of 2,2'-dipyridyl before growth was monitored for 44 hours. Culture treated with 50 μM 2,2'–dipyridyl showed a slight reduction in growth and this concentration was therefore used in all subsequent experiments.
    Nacl, supplied by Difco, used in various techniques. Bioz Stars score: 94/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nacl/product/Difco
    Average 94 stars, based on 64 article reviews
    Price from $9.99 to $1999.99
    nacl - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    84
    Difco luria bertani lb medium medium 10 g of bacto tryptone
    Titration of 2,2’-dipyridyl concentration. A. <t>salmonicida</t> strain LFI1238 was grown in LB containing 1% <t>NaCl</t> to an optical density at 0.4 (600 nm). The culture was split into six individual flasks and supplemented with different concentrations of 2,2'-dipyridyl before growth was monitored for 44 hours. Culture treated with 50 μM 2,2'–dipyridyl showed a slight reduction in growth and this concentration was therefore used in all subsequent experiments.
    Luria Bertani Lb Medium Medium 10 G Of Bacto Tryptone, supplied by Difco, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luria bertani lb medium medium 10 g of bacto tryptone/product/Difco
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    luria bertani lb medium medium 10 g of bacto tryptone - by Bioz Stars, 2020-04
    84/100 stars
      Buy from Supplier

    94
    Difco luria bertani lb medium
    Titration of 2,2’-dipyridyl concentration. A. <t>salmonicida</t> strain LFI1238 was grown in LB containing 1% <t>NaCl</t> to an optical density at 0.4 (600 nm). The culture was split into six individual flasks and supplemented with different concentrations of 2,2'-dipyridyl before growth was monitored for 44 hours. Culture treated with 50 μM 2,2'–dipyridyl showed a slight reduction in growth and this concentration was therefore used in all subsequent experiments.
    Luria Bertani Lb Medium, supplied by Difco, used in various techniques. Bioz Stars score: 94/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luria bertani lb medium/product/Difco
    Average 94 stars, based on 39 article reviews
    Price from $9.99 to $1999.99
    luria bertani lb medium - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

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    Titration of 2,2’-dipyridyl concentration. A. salmonicida strain LFI1238 was grown in LB containing 1% NaCl to an optical density at 0.4 (600 nm). The culture was split into six individual flasks and supplemented with different concentrations of 2,2'-dipyridyl before growth was monitored for 44 hours. Culture treated with 50 μM 2,2'–dipyridyl showed a slight reduction in growth and this concentration was therefore used in all subsequent experiments.

    Journal: BMC Microbiology

    Article Title: The immediate global responses of Aliivibrio salmonicida to iron limitations

    doi: 10.1186/s12866-015-0342-7

    Figure Lengend Snippet: Titration of 2,2’-dipyridyl concentration. A. salmonicida strain LFI1238 was grown in LB containing 1% NaCl to an optical density at 0.4 (600 nm). The culture was split into six individual flasks and supplemented with different concentrations of 2,2'-dipyridyl before growth was monitored for 44 hours. Culture treated with 50 μM 2,2'–dipyridyl showed a slight reduction in growth and this concentration was therefore used in all subsequent experiments.

    Article Snippet: Bacterial strains, culture conditions and sampling A. salmonicida strain LFI1238 [ ] was cultured in LB medium containing 1% NaCl (Luria-Bertani broth Miller, Difco) at 8°C with 200 rpm in all experiments.

    Techniques: Titration, Concentration Assay