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Difco luria bertani broth lb
Luria Bertani Broth Lb, supplied by Difco, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 2 article reviews
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luria bertani broth lb - by Bioz Stars, 2020-04
94/100 stars

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Related Articles

Clone Assay:

Article Title: Quorum sensing is a key regulator for the antifungal and biocontrol activity of chitinase‐producing Chromobacterium sp. C61
Article Snippet: Chromobacterium strain C61 was grown in nutrient broth (NB; Difco, Detroit, MI, USA) or Luria–Bertani broth (LB; Difco) (Sambrook et al ., ) containing 50 μg/mL ampicillin at 28 °C. .. To isolate mutants of Chromobacterium C61 defective in antifungal activity, a mutant library of Chromobacterium C61 containing 2000 clones was generated by random insertion of a Tn 5 transposon from the PRL1063a vector.

Article Title: Evaluation of Clumping Factor A Binding Region A in a Subunit Vaccine against Staphylococcus aureus-Induced Mastitis in Mice ▿
Article Snippet: .. Escherichia coli strain DH5α grown in Luria-Bertani broth or agar (Difco) at 37°C in the presence of 50 μg/ml kanamycin when necessary was used as the host for cloning. .. Genomic DNA extracted from S. aureus strain J9 using an extraction kit (Clontech, Palo Alto, CA) was used as the PCR template.

Selection:

Article Title: Molecular determinants for CRISPR RNA maturation in the Cas10–Csm complex and roles for non-Cas nucleases
Article Snippet: E. coli DH5α and BL21 (DE3) codon plus cells (EMD Millipore) were grown in Luria Bertani broth (LB, Difco) or Terrific broth (Amresco) for protein purification. .. Media was supplemented with the following as needed: 10 μg/ml chloramphenicol (for selection of pcrispr -based plasmids), 15 μg/mL neomycin (for selection of S. epidermidis ), 5 μg/ml mupirocin (for selection of pG0400), 30 μg/ml chloramphenicol (for selection of E. coli BL21 (DE3)) and 50 μg/ml kanamycin (for selection of pET28b-based plasmids).

Electron Microscopy:

Article Title: Mutations in the csgD Promoter Associated with Variations in Curli Expression in Certain Strains of Escherichia coli O157:H7
Article Snippet: Red colonies were dry, rough, and curliated as verified by electron microscopy (results not shown). .. Red variants passaged daily (1:100) in Luria-Bertani broth (Difco Laboratories, Detroit, Mich.) at 37°C generated mixed red and white phenotypes in as few as 3 passages, with white variants persisting at 40 to 60% of total colonies over 10 passages.

Mutagenesis:

Article Title: Quorum sensing is a key regulator for the antifungal and biocontrol activity of chitinase‐producing Chromobacterium sp. C61
Article Snippet: Chromobacterium strain C61 was grown in nutrient broth (NB; Difco, Detroit, MI, USA) or Luria–Bertani broth (LB; Difco) (Sambrook et al ., ) containing 50 μg/mL ampicillin at 28 °C. .. To isolate mutants of Chromobacterium C61 defective in antifungal activity, a mutant library of Chromobacterium C61 containing 2000 clones was generated by random insertion of a Tn 5 transposon from the PRL1063a vector.

Isolation:

Article Title: Evaluation of Clumping Factor A Binding Region A in a Subunit Vaccine against Staphylococcus aureus-Induced Mastitis in Mice ▿
Article Snippet: S. aureus strain J9 was isolated and identified from a case of bovine mastitis in Dongxihu District, Wuhan City, China. .. Escherichia coli strain DH5α grown in Luria-Bertani broth or agar (Difco) at 37°C in the presence of 50 μg/ml kanamycin when necessary was used as the host for cloning.

Article Title: Detection, Isolation, and Identification of Vibrio cholerae from the Environment
Article Snippet: With a sterile loop or toothpick dab the upper pellicle layer of the incubated sample and streak for isolation onto a selective medium for V. cholerae isolation (TTGA, TCBS, or CA). .. For arginine dihydrolase assay simultaneously inoculate Luria-Bertani broth containing 1% (wt/vol) L-arginine (pH 6.8) and phenol red powder (Difco) added as an indicator.

Generated:

Article Title: Quorum sensing is a key regulator for the antifungal and biocontrol activity of chitinase‐producing Chromobacterium sp. C61
Article Snippet: Chromobacterium strain C61 was grown in nutrient broth (NB; Difco, Detroit, MI, USA) or Luria–Bertani broth (LB; Difco) (Sambrook et al ., ) containing 50 μg/mL ampicillin at 28 °C. .. To isolate mutants of Chromobacterium C61 defective in antifungal activity, a mutant library of Chromobacterium C61 containing 2000 clones was generated by random insertion of a Tn 5 transposon from the PRL1063a vector.

Article Title: Mutations in the csgD Promoter Associated with Variations in Curli Expression in Certain Strains of Escherichia coli O157:H7
Article Snippet: .. Red variants passaged daily (1:100) in Luria-Bertani broth (Difco Laboratories, Detroit, Mich.) at 37°C generated mixed red and white phenotypes in as few as 3 passages, with white variants persisting at 40 to 60% of total colonies over 10 passages. .. White variants were stable under all growth conditions tested except for one, strain ATCC 43894 (stored frozen for 6 months at −80°C in brain heart infusion broth with 15% glycerol), which generated rare (10−4 ) red variants.

Cell Culture:

Article Title: Intracellular Induction of the Bartonella henselae virB Operon by Human Endothelial Cells
Article Snippet: Human microvascular endothelial cells (HMEC-1) ( ) were cultured in MCBD131 cell culture media (Gibco/BRL, Grand Island, N.Y.) supplemented with 10% fetal bovine serum, 10 ng of epidermal growth factor/ml, and 1 μg of hydrocortisone/ml. .. Escherichia coli strains were grown using Luria-Bertani broth or agar (Difco, Detroit, Mich.) with appropriate antibiotics (ampicillin, 100 μg/ml; kanamycin, 50 μg/ml).

Protein Purification:

Article Title: Molecular determinants for CRISPR RNA maturation in the Cas10–Csm complex and roles for non-Cas nucleases
Article Snippet: .. E. coli DH5α and BL21 (DE3) codon plus cells (EMD Millipore) were grown in Luria Bertani broth (LB, Difco) or Terrific broth (Amresco) for protein purification. .. Media was supplemented with the following as needed: 10 μg/ml chloramphenicol (for selection of pcrispr -based plasmids), 15 μg/mL neomycin (for selection of S. epidermidis ), 5 μg/ml mupirocin (for selection of pG0400), 30 μg/ml chloramphenicol (for selection of E. coli BL21 (DE3)) and 50 μg/ml kanamycin (for selection of pET28b-based plasmids).

Polymerase Chain Reaction:

Article Title: Evaluation of Clumping Factor A Binding Region A in a Subunit Vaccine against Staphylococcus aureus-Induced Mastitis in Mice ▿
Article Snippet: The identity of the strain was confirmed by PCR and 16S rRNA sequencing. .. Escherichia coli strain DH5α grown in Luria-Bertani broth or agar (Difco) at 37°C in the presence of 50 μg/ml kanamycin when necessary was used as the host for cloning.

Incubation:

Article Title: Effects of growth conditions on biofilm formation by Actinobacillus pleuropneumoniae
Article Snippet: .. A colony was transferred into 5 mL of Luria-Bertani broth (LB; Difco), tryptic soy broth (TSB; Difco), Mueller Hinton broth (MH; Difco) or BHI (BHI-A; Difco or BHI-B; Oxoid Ltd, Basingstoke, Hampshire, UK) with 5 μg/mL NAD and incubated at 37 °C overnight with agitation. ..

Article Title: Intracellular Induction of the Bartonella henselae virB Operon by Human Endothelial Cells
Article Snippet: Escherichia coli strains were grown using Luria-Bertani broth or agar (Difco, Detroit, Mich.) with appropriate antibiotics (ampicillin, 100 μg/ml; kanamycin, 50 μg/ml). .. For some experiments, B. henselae strains were incubated with supplemented MCBD131 at 37°C in 5% CO2 .

Article Title: Detection, Isolation, and Identification of Vibrio cholerae from the Environment
Article Snippet: With a sterile loop or toothpick dab the upper pellicle layer of the incubated sample and streak for isolation onto a selective medium for V. cholerae isolation (TTGA, TCBS, or CA). .. For arginine dihydrolase assay simultaneously inoculate Luria-Bertani broth containing 1% (wt/vol) L-arginine (pH 6.8) and phenol red powder (Difco) added as an indicator.

other:

Article Title: Cloning, Characterization, and Inactivation of the Gene pbpC, Encoding Penicillin-Binding Protein 3 of Staphylococcus aureus
Article Snippet: Escherichia coli strains were grown in Luria-Bertani broth (Difco) with aeration.

Article Title: Chemical Genetic Analysis and Functional Characterization of Staphylococcal Wall Teichoic Acid 2-Epimerases Reveals Unconventional Antibiotic Drug Targets
Article Snippet: MRSE strains were tested in Luria Bertani broth (Difco).

Article Title: Reconstitution of the Mycobacterium tuberculosis pupylation pathway in Escherichia coli
Article Snippet: E. coli strains were grown in Luria–Bertani broth or on Luria–Bertani-agar plates (Difco).

Article Title: Development of a Thermally Regulated Broad-Spectrum Promoter System for Use in Pathogenic Gram-Positive Species
Article Snippet: The following growth media (Difco) were used: Luria-Bertani broth for E. coli , tryptic soy broth for S. aureus , brain heart infusion broth for E. faecalis , and Todd-Hewitt broth for E. faecium .

Article Title: Imipenem Resistance of Enterobacter aerogenes Mediated by Outer Membrane Permeability
Article Snippet: Exponential-phase bacterial cells grown in Luria-Bertani broth were collected.

Activity Assay:

Article Title: Quorum sensing is a key regulator for the antifungal and biocontrol activity of chitinase‐producing Chromobacterium sp. C61
Article Snippet: Paragraph title: Identification of C61 Mutants Defective in Antifungal Activity ... Chromobacterium strain C61 was grown in nutrient broth (NB; Difco, Detroit, MI, USA) or Luria–Bertani broth (LB; Difco) (Sambrook et al ., ) containing 50 μg/mL ampicillin at 28 °C.

Plasmid Preparation:

Article Title: Quorum sensing is a key regulator for the antifungal and biocontrol activity of chitinase‐producing Chromobacterium sp. C61
Article Snippet: Chromobacterium strain C61 was grown in nutrient broth (NB; Difco, Detroit, MI, USA) or Luria–Bertani broth (LB; Difco) (Sambrook et al ., ) containing 50 μg/mL ampicillin at 28 °C. .. To isolate mutants of Chromobacterium C61 defective in antifungal activity, a mutant library of Chromobacterium C61 containing 2000 clones was generated by random insertion of a Tn 5 transposon from the PRL1063a vector.

Expressing:

Article Title: Mutations in the csgD Promoter Associated with Variations in Curli Expression in Certain Strains of Escherichia coli O157:H7
Article Snippet: Red and white colonies retained their parental phenotypes when subcultured on CRI plates with or without 1% NaCl and at either 28 or 37°C, suggesting that curli expression was neither low-temperature nor low-osmolarity dependent. .. Red variants passaged daily (1:100) in Luria-Bertani broth (Difco Laboratories, Detroit, Mich.) at 37°C generated mixed red and white phenotypes in as few as 3 passages, with white variants persisting at 40 to 60% of total colonies over 10 passages.

Sequencing:

Article Title: Evaluation of Clumping Factor A Binding Region A in a Subunit Vaccine against Staphylococcus aureus-Induced Mastitis in Mice ▿
Article Snippet: The identity of the strain was confirmed by PCR and 16S rRNA sequencing. .. Escherichia coli strain DH5α grown in Luria-Bertani broth or agar (Difco) at 37°C in the presence of 50 μg/ml kanamycin when necessary was used as the host for cloning.

Derivative Assay:

Article Title: Mutations in the csgD Promoter Associated with Variations in Curli Expression in Certain Strains of Escherichia coli O157:H7
Article Snippet: Red variants passaged daily (1:100) in Luria-Bertani broth (Difco Laboratories, Detroit, Mich.) at 37°C generated mixed red and white phenotypes in as few as 3 passages, with white variants persisting at 40 to 60% of total colonies over 10 passages. .. Red and white colonies derived from CRI plate passage of strains ATCC 43895 and ATCC 43894 ( n = 16) (Fig. ), Tarr1A, Tarr4A, and two randomly selected bovine isolates (strains 84-2 and 161-2) were further analyzed.

Hydrolysis Assay:

Article Title: Detection, Isolation, and Identification of Vibrio cholerae from the Environment
Article Snippet: For esculin hydrolysis assay gently touch the top and center of a yellow non-swarming colony with a sterile loop or toothpick and inoculate into heart infusion agar containing 0.1% esculin and 0.05% ferric chloride. .. For arginine dihydrolase assay simultaneously inoculate Luria-Bertani broth containing 1% (wt/vol) L-arginine (pH 6.8) and phenol red powder (Difco) added as an indicator.

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  • 97
    Difco luria bertani lb broth
    Heat map of microarray analysis and the corresponding values of fold change for the 70 significantly upregulated or downregulated genes of in vitro M cells and Caco-2 cells after infection with wild-type (WT) S . Typhimurium and Δ speG or remaining uninfected for 1 h. We found 70 genes of in vitro M cells and Caco-2 cells that were significantly upregulated ( > 2-fold change, red bricks) after treatment with WT S . Typhimurium SL1344, the speG -deleted mutant (Δ speG ), and only <t>Luria-Bertani</t> broth (LB). These 70 genes were classified into 8 groups according to their relative or potential functions. Red bricks represent higher gene expression levels compared with average levels (black bricks).
    Luria Bertani Lb Broth, supplied by Difco, used in various techniques. Bioz Stars score: 97/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luria bertani lb broth/product/Difco
    Average 97 stars, based on 35 article reviews
    Price from $9.99 to $1999.99
    luria bertani lb broth - by Bioz Stars, 2020-04
    97/100 stars
      Buy from Supplier

    95
    Difco luria bertani lb
    Escherichia coli growth curve in the presence of chitosan polymers and nanoparticles. The DH5α strain was grown in <t>Luria–Bertani</t> medium at 37 °C and 180 rpm. The black arrow indicates the addition of medium (M), and low molecular (L) polymers or MN LN nanoparticles in order to reach final concentration of 3 × minimum inhibitory concentration (MIC) values, of 1.41, 0.9, 1.2 and 0.9 mg/mL, of CS polymers/nanoparticles respectively.
    Luria Bertani Lb, supplied by Difco, used in various techniques. Bioz Stars score: 95/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luria bertani lb/product/Difco
    Average 95 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    luria bertani lb - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    Image Search Results


    Heat map of microarray analysis and the corresponding values of fold change for the 70 significantly upregulated or downregulated genes of in vitro M cells and Caco-2 cells after infection with wild-type (WT) S . Typhimurium and Δ speG or remaining uninfected for 1 h. We found 70 genes of in vitro M cells and Caco-2 cells that were significantly upregulated ( > 2-fold change, red bricks) after treatment with WT S . Typhimurium SL1344, the speG -deleted mutant (Δ speG ), and only Luria-Bertani broth (LB). These 70 genes were classified into 8 groups according to their relative or potential functions. Red bricks represent higher gene expression levels compared with average levels (black bricks).

    Journal: PLoS ONE

    Article Title: Impacts of Salmonella enterica Serovar Typhimurium and Its speG Gene on the Transcriptomes of In Vitro M Cells and Caco-2 Cells

    doi: 10.1371/journal.pone.0153444

    Figure Lengend Snippet: Heat map of microarray analysis and the corresponding values of fold change for the 70 significantly upregulated or downregulated genes of in vitro M cells and Caco-2 cells after infection with wild-type (WT) S . Typhimurium and Δ speG or remaining uninfected for 1 h. We found 70 genes of in vitro M cells and Caco-2 cells that were significantly upregulated ( > 2-fold change, red bricks) after treatment with WT S . Typhimurium SL1344, the speG -deleted mutant (Δ speG ), and only Luria-Bertani broth (LB). These 70 genes were classified into 8 groups according to their relative or potential functions. Red bricks represent higher gene expression levels compared with average levels (black bricks).

    Article Snippet: For cell infections, the single colonies of each strains were aerobically grown in 3 mL of Luria–Bertani (LB) broth (Difco) at 37°C for 18 h to reach a bacterial density of 1 × 109 CFU/mL as overnight cultures.

    Techniques: Microarray, In Vitro, Infection, Mutagenesis, Expressing

    Population growth curves of the bacterial isolate C1-7 with the time after culturing in different media including brain heart infusion broth (BHIB), Luria–Bertani broth (LBB), tryptic soy broth (TSB), and nutrient broth (NB). Vertical lines denote standard deviations of three replications.

    Journal: The Plant Pathology Journal

    Article Title: Biological Control of Meloidogyne hapla Using an Antagonistic Bacterium

    doi: 10.5423/PPJ.OA.02.2014.0013

    Figure Lengend Snippet: Population growth curves of the bacterial isolate C1-7 with the time after culturing in different media including brain heart infusion broth (BHIB), Luria–Bertani broth (LBB), tryptic soy broth (TSB), and nutrient broth (NB). Vertical lines denote standard deviations of three replications.

    Article Snippet: Growth characteristics of C1-7 in different culture media To determine the optimal population density and culture media for the most efficient application of isolate C1-7, growth curves were examined every 6 h for 114 h after inoculation on four different media including nutrient broth (NB; Conda S.A.), BHIB, Luria–Bertani (LB) broth (LBB; Difco, Detroit, MI, USA), and Bacto™ tryptic soy (TS) broth (TSB; Becton, Dickinson and Company, Le Pont de Claix Cedex, France).

    Techniques:

    Biofilm formation by A . pleuropneumoniae serotype 1 reference strain S4074 grown in different culture media using the crystal violet staining protocol described in Materials and methods. LB: Luria-Bertani; TSB: tryptic soy broth; M-H: Mueller Hinton; BHI: brain heart infusion.

    Journal: Veterinary Research

    Article Title: Effects of growth conditions on biofilm formation by Actinobacillus pleuropneumoniae

    doi: 10.1051/vetres/2009051

    Figure Lengend Snippet: Biofilm formation by A . pleuropneumoniae serotype 1 reference strain S4074 grown in different culture media using the crystal violet staining protocol described in Materials and methods. LB: Luria-Bertani; TSB: tryptic soy broth; M-H: Mueller Hinton; BHI: brain heart infusion.

    Article Snippet: A colony was transferred into 5 mL of Luria-Bertani broth (LB; Difco), tryptic soy broth (TSB; Difco), Mueller Hinton broth (MH; Difco) or BHI (BHI-A; Difco or BHI-B; Oxoid Ltd, Basingstoke, Hampshire, UK) with 5 μg/mL NAD and incubated at 37 °C overnight with agitation.

    Techniques: Staining

    Escherichia coli growth curve in the presence of chitosan polymers and nanoparticles. The DH5α strain was grown in Luria–Bertani medium at 37 °C and 180 rpm. The black arrow indicates the addition of medium (M), and low molecular (L) polymers or MN LN nanoparticles in order to reach final concentration of 3 × minimum inhibitory concentration (MIC) values, of 1.41, 0.9, 1.2 and 0.9 mg/mL, of CS polymers/nanoparticles respectively.

    Journal: International Journal of Molecular Sciences

    Article Title: Proteomic Analyses Reveal New Insights on the Antimicrobial Mechanisms of Chitosan Biopolymers and Their Nanosized Particles against Escherichia coli

    doi: 10.3390/ijms21010225

    Figure Lengend Snippet: Escherichia coli growth curve in the presence of chitosan polymers and nanoparticles. The DH5α strain was grown in Luria–Bertani medium at 37 °C and 180 rpm. The black arrow indicates the addition of medium (M), and low molecular (L) polymers or MN LN nanoparticles in order to reach final concentration of 3 × minimum inhibitory concentration (MIC) values, of 1.41, 0.9, 1.2 and 0.9 mg/mL, of CS polymers/nanoparticles respectively.

    Article Snippet: Bacterial Strain and Culture The Escherichia coli DH5α strain was streaked on Luria-Bertani (LB) (Difco™ LB Broth, Miller BD, NJ, USA) agar plates and grown at 37 °C.

    Techniques: Concentration Assay