Structured Review

Phenomenex luna hilic column
( A ) Total ion chromatography of <t>HILIC-FTMS</t> profiling of fully digested KS domain structure. ( B ) Composition analysis of <t>KSO</t> calculated by GlycResoft. The analytical error for each GAG was
Luna Hilic Column, supplied by Phenomenex, used in various techniques. Bioz Stars score: 90/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/luna hilic column/product/Phenomenex
Average 90 stars, based on 62 article reviews
Price from $9.99 to $1999.99
luna hilic column - by Bioz Stars, 2020-07
90/100 stars

Images

1) Product Images from "Structural Characterization and Interaction with RCA120 of a Highly Sulfated Keratan Sulfate from Blue Shark (Prionace glauca) Cartilage"

Article Title: Structural Characterization and Interaction with RCA120 of a Highly Sulfated Keratan Sulfate from Blue Shark (Prionace glauca) Cartilage

Journal: Marine Drugs

doi: 10.3390/md16040128

( A ) Total ion chromatography of HILIC-FTMS profiling of fully digested KS domain structure. ( B ) Composition analysis of KSO calculated by GlycResoft. The analytical error for each GAG was
Figure Legend Snippet: ( A ) Total ion chromatography of HILIC-FTMS profiling of fully digested KS domain structure. ( B ) Composition analysis of KSO calculated by GlycResoft. The analytical error for each GAG was

Techniques Used: Ion Chromatography, Hydrophilic Interaction Liquid Chromatography

2) Product Images from "Bottom-up analysis using liquid chromatography–Fourier transform mass spectrometry to characterize fucosylated chondroitin sulfates from sea cucumbers"

Article Title: Bottom-up analysis using liquid chromatography–Fourier transform mass spectrometry to characterize fucosylated chondroitin sulfates from sea cucumbers

Journal: Glycobiology

doi: 10.1093/glycob/cwz057

TICs of depolymerized FCS by partial N -deacetylation–deaminative cleavage from four species of sea cucumbers profiled by HILIC-FTMS.
Figure Legend Snippet: TICs of depolymerized FCS by partial N -deacetylation–deaminative cleavage from four species of sea cucumbers profiled by HILIC-FTMS.

Techniques Used: Hydrophilic Interaction Liquid Chromatography

3) Product Images from "Preparation of low molecular weight heparin using an ultrasound-assisted Fenton-system"

Article Title: Preparation of low molecular weight heparin using an ultrasound-assisted Fenton-system

Journal: Ultrasonics sonochemistry

doi: 10.1016/j.ultsonch.2018.11.016

Top-down approach using HILIC LC–FT–ESI–MS to characterize LMWH-1 and LMWH-4. (a) Detected LMWH-1 oligosaccharides. (b) Detected LMWH-4 oligosaccharides. The oligosaccharides are labeled with the number of [HexA, HexA, GlcN, Ac, SO 3 ] moieties present in each structure.
Figure Legend Snippet: Top-down approach using HILIC LC–FT–ESI–MS to characterize LMWH-1 and LMWH-4. (a) Detected LMWH-1 oligosaccharides. (b) Detected LMWH-4 oligosaccharides. The oligosaccharides are labeled with the number of [HexA, HexA, GlcN, Ac, SO 3 ] moieties present in each structure.

Techniques Used: Hydrophilic Interaction Liquid Chromatography, Labeling

4) Product Images from "Structural Characterization and Interaction with RCA120 of a Highly Sulfated Keratan Sulfate from Blue Shark (Prionace glauca) Cartilage"

Article Title: Structural Characterization and Interaction with RCA120 of a Highly Sulfated Keratan Sulfate from Blue Shark (Prionace glauca) Cartilage

Journal: Marine Drugs

doi: 10.3390/md16040128

( A ) Total ion chromatography of HILIC-FTMS profiling of fully digested KS domain structure. ( B ) Composition analysis of KSO calculated by GlycResoft. The analytical error for each GAG was
Figure Legend Snippet: ( A ) Total ion chromatography of HILIC-FTMS profiling of fully digested KS domain structure. ( B ) Composition analysis of KSO calculated by GlycResoft. The analytical error for each GAG was

Techniques Used: Ion Chromatography, Hydrophilic Interaction Liquid Chromatography

5) Product Images from "Bottom-Up Low Molecular Weight Heparin Analysis Using Liquid Chromatography-Fourier Transform Mass Spectrometry for Extensive Characterization"

Article Title: Bottom-Up Low Molecular Weight Heparin Analysis Using Liquid Chromatography-Fourier Transform Mass Spectrometry for Extensive Characterization

Journal: Analytical Chemistry

doi: 10.1021/ac501301v

HILIC LC-FTMS total ion chromatogram (TIC) and major heparin lyase II-derived oligosaccharides quantified from the LMWHs of three different manufacturers. (A) TIC of digested LMWH, reproducibility of the HILIC LC-FTMS method followed by enzyme digestion. Triplicated sample preparations were analyzed on the same LC-MS batch. (B) Typical unsaturated oligosaccharide products quantification comparison after digestion of LMWHs from three different manufacturers. Figure inset is the magnified profile of low abundance oligosaccharides.
Figure Legend Snippet: HILIC LC-FTMS total ion chromatogram (TIC) and major heparin lyase II-derived oligosaccharides quantified from the LMWHs of three different manufacturers. (A) TIC of digested LMWH, reproducibility of the HILIC LC-FTMS method followed by enzyme digestion. Triplicated sample preparations were analyzed on the same LC-MS batch. (B) Typical unsaturated oligosaccharide products quantification comparison after digestion of LMWHs from three different manufacturers. Figure inset is the magnified profile of low abundance oligosaccharides.

Techniques Used: Hydrophilic Interaction Liquid Chromatography, Derivative Assay, Liquid Chromatography with Mass Spectroscopy

Related Articles

High Performance Liquid Chromatography:

Article Title: Fatty acid composition and phospholipid types used in infant formulas modifies the establishment of human gut bacteria in germ-free mice
Article Snippet: .. Characterization of emulsifiers SL and purified MPL were characterized by HPLC essentially as described by Silversand and Haux , using a Luna HILIC column (100 × 3 mm) (Phenomenex) on an Agilent 1100 HPLC system with a Polymer Labs evaporative light scattering detector PL-ELS 2100 operated with an N2 flow of 1.90 ml/min, and an evaporator temperature of 90 °C and a nebulizer temperature of 75 °C. ..

Article Title: The botanical drug substance crofelemer as a model system for comparative characterization of complex mixture drugs
Article Snippet: .. HILIC HPLC was performed with a Luna HILIC column (2 × 150 mm, Phenomenex, Torrance, CA) on a Shimadzu LC 2010C HT HPLC system with a Shimadzu SPD-M20A diode array detector. ..

Hydrophilic Interaction Liquid Chromatography:

Article Title: Fatty acid composition and phospholipid types used in infant formulas modifies the establishment of human gut bacteria in germ-free mice
Article Snippet: .. Characterization of emulsifiers SL and purified MPL were characterized by HPLC essentially as described by Silversand and Haux , using a Luna HILIC column (100 × 3 mm) (Phenomenex) on an Agilent 1100 HPLC system with a Polymer Labs evaporative light scattering detector PL-ELS 2100 operated with an N2 flow of 1.90 ml/min, and an evaporator temperature of 90 °C and a nebulizer temperature of 75 °C. ..

Article Title: [18F]fluorocholine PET/CT imaging of liver cancer: radiopathologic correlation with tissue phospholipid profiling
Article Snippet: .. Tissues were analyzed using liquid chromatography-mass spectrometry (LCMS) by injecting 10 μL of tissue extract via a PAL autosampler onto a Luna HILIC column (150mm × 3mm; 3 μm; Phenomenex) containing a pre-column filter (0.2 μm; Thermo). .. A linear gradient elution was performed using MeCN (solvent A) and 40mM aqueous ammonium acetate at pH 3.5 (solvent B) at 0.5 mL/minute as follows: 0–10 minutes from 5% to 30% B, then hold at 30% B for 16 minutes before equilibrating with 5% B for 7 minutes.

Article Title: Structural Characterization and Interaction with RCA120 of a Highly Sulfated Keratan Sulfate from Blue Shark (Prionace glauca) Cartilage
Article Snippet: .. The KSO were separated by a Luna HILIC column (150 × 2.00 mm, 3 μm, Phenomenex) at 25 °C. .. The mobile phase was a mixture of 5 mM NH4 OAc/98% acetonitrile (solvent A) and 5 mM NH4 OAc/H2 O (solvent B) at a flow rate of 150 μL/min.

Article Title: Preparation of low molecular weight heparin using an ultrasound-assisted Fenton-system
Article Snippet: .. LMWH-1 and LMWH-4 were separated using a Luna HILIC column (2.0 × 150 mm2 , 200 Å, Phenomenex, Torrance, CA). ..

Article Title: The botanical drug substance crofelemer as a model system for comparative characterization of complex mixture drugs
Article Snippet: .. HILIC HPLC was performed with a Luna HILIC column (2 × 150 mm, Phenomenex, Torrance, CA) on a Shimadzu LC 2010C HT HPLC system with a Shimadzu SPD-M20A diode array detector. ..

Article Title: Bottom-Up Low Molecular Weight Heparin Analysis Using Liquid Chromatography-Fourier Transform Mass Spectrometry for Extensive Characterization
Article Snippet: .. HILIC LC ESI-LTQ-Orbitrap-FTMS Analysis of Digested LMWHs A Luna HILIC column (2.0 × 50 mm, 200 Å, Phenomenex, Torrance, CA) was used to separate the LMWHs. .. Mobile phase A was 5 mM ammonium acetate prepared with HPLC grade water.

Article Title: Bottom-up analysis using liquid chromatography–Fourier transform mass spectrometry to characterize fucosylated chondroitin sulfates from sea cucumbers
Article Snippet: .. The FCS oligosaccharides were separated by a Luna HILIC column (150 × 2.00 mm, 3 μm, Phenomenex) at 25°C. .. The mobile phase was a mixture of 5 mM NH4 OAc/98% acetonitrile (solvent A) and 5 mM NH4 OAc/H2 O (solvent B) at a flow rate of 150 μL/min.

Flow Cytometry:

Article Title: Fatty acid composition and phospholipid types used in infant formulas modifies the establishment of human gut bacteria in germ-free mice
Article Snippet: .. Characterization of emulsifiers SL and purified MPL were characterized by HPLC essentially as described by Silversand and Haux , using a Luna HILIC column (100 × 3 mm) (Phenomenex) on an Agilent 1100 HPLC system with a Polymer Labs evaporative light scattering detector PL-ELS 2100 operated with an N2 flow of 1.90 ml/min, and an evaporator temperature of 90 °C and a nebulizer temperature of 75 °C. ..

Chromatography:

Article Title: [18F]fluorocholine PET/CT imaging of liver cancer: radiopathologic correlation with tissue phospholipid profiling
Article Snippet: .. Tissues were analyzed using liquid chromatography-mass spectrometry (LCMS) by injecting 10 μL of tissue extract via a PAL autosampler onto a Luna HILIC column (150mm × 3mm; 3 μm; Phenomenex) containing a pre-column filter (0.2 μm; Thermo). .. A linear gradient elution was performed using MeCN (solvent A) and 40mM aqueous ammonium acetate at pH 3.5 (solvent B) at 0.5 mL/minute as follows: 0–10 minutes from 5% to 30% B, then hold at 30% B for 16 minutes before equilibrating with 5% B for 7 minutes.

Purification:

Article Title: Fatty acid composition and phospholipid types used in infant formulas modifies the establishment of human gut bacteria in germ-free mice
Article Snippet: .. Characterization of emulsifiers SL and purified MPL were characterized by HPLC essentially as described by Silversand and Haux , using a Luna HILIC column (100 × 3 mm) (Phenomenex) on an Agilent 1100 HPLC system with a Polymer Labs evaporative light scattering detector PL-ELS 2100 operated with an N2 flow of 1.90 ml/min, and an evaporator temperature of 90 °C and a nebulizer temperature of 75 °C. ..

Liquid Chromatography with Mass Spectroscopy:

Article Title: [18F]fluorocholine PET/CT imaging of liver cancer: radiopathologic correlation with tissue phospholipid profiling
Article Snippet: .. Tissues were analyzed using liquid chromatography-mass spectrometry (LCMS) by injecting 10 μL of tissue extract via a PAL autosampler onto a Luna HILIC column (150mm × 3mm; 3 μm; Phenomenex) containing a pre-column filter (0.2 μm; Thermo). .. A linear gradient elution was performed using MeCN (solvent A) and 40mM aqueous ammonium acetate at pH 3.5 (solvent B) at 0.5 mL/minute as follows: 0–10 minutes from 5% to 30% B, then hold at 30% B for 16 minutes before equilibrating with 5% B for 7 minutes.

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  • 90
    Phenomenex luna hilic column
    ( A ) Total ion chromatography of <t>HILIC-FTMS</t> profiling of fully digested KS domain structure. ( B ) Composition analysis of <t>KSO</t> calculated by GlycResoft. The analytical error for each GAG was
    Luna Hilic Column, supplied by Phenomenex, used in various techniques. Bioz Stars score: 90/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luna hilic column/product/Phenomenex
    Average 90 stars, based on 62 article reviews
    Price from $9.99 to $1999.99
    luna hilic column - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    91
    Phenomenex hilic column
    Extracted ion chromatogram (XIC) of acylPG in corn roots obtained from ( a ) <t>HILIC</t> and ( b ) <t>C30-RPLC,</t> ( c ) HILIC-MS of total acylPG. Corresponding HRMS/MS spectrum of m/z 1029.76 obtained from ( d ) HILIC and ( e , f ) C30-RPLC. Structures of acylPG are inserted in the MS/MS spectra.
    Hilic Column, supplied by Phenomenex, used in various techniques. Bioz Stars score: 91/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hilic column/product/Phenomenex
    Average 91 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    hilic column - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    Image Search Results


    ( A ) Total ion chromatography of HILIC-FTMS profiling of fully digested KS domain structure. ( B ) Composition analysis of KSO calculated by GlycResoft. The analytical error for each GAG was

    Journal: Marine Drugs

    Article Title: Structural Characterization and Interaction with RCA120 of a Highly Sulfated Keratan Sulfate from Blue Shark (Prionace glauca) Cartilage

    doi: 10.3390/md16040128

    Figure Lengend Snippet: ( A ) Total ion chromatography of HILIC-FTMS profiling of fully digested KS domain structure. ( B ) Composition analysis of KSO calculated by GlycResoft. The analytical error for each GAG was

    Article Snippet: The KSO were separated by a Luna HILIC column (150 × 2.00 mm, 3 μm, Phenomenex) at 25 °C.

    Techniques: Ion Chromatography, Hydrophilic Interaction Liquid Chromatography

    TICs of depolymerized FCS by partial N -deacetylation–deaminative cleavage from four species of sea cucumbers profiled by HILIC-FTMS.

    Journal: Glycobiology

    Article Title: Bottom-up analysis using liquid chromatography–Fourier transform mass spectrometry to characterize fucosylated chondroitin sulfates from sea cucumbers

    doi: 10.1093/glycob/cwz057

    Figure Lengend Snippet: TICs of depolymerized FCS by partial N -deacetylation–deaminative cleavage from four species of sea cucumbers profiled by HILIC-FTMS.

    Article Snippet: The FCS oligosaccharides were separated by a Luna HILIC column (150 × 2.00 mm, 3 μm, Phenomenex) at 25°C.

    Techniques: Hydrophilic Interaction Liquid Chromatography

    Top-down approach using HILIC LC–FT–ESI–MS to characterize LMWH-1 and LMWH-4. (a) Detected LMWH-1 oligosaccharides. (b) Detected LMWH-4 oligosaccharides. The oligosaccharides are labeled with the number of [HexA, HexA, GlcN, Ac, SO 3 ] moieties present in each structure.

    Journal: Ultrasonics sonochemistry

    Article Title: Preparation of low molecular weight heparin using an ultrasound-assisted Fenton-system

    doi: 10.1016/j.ultsonch.2018.11.016

    Figure Lengend Snippet: Top-down approach using HILIC LC–FT–ESI–MS to characterize LMWH-1 and LMWH-4. (a) Detected LMWH-1 oligosaccharides. (b) Detected LMWH-4 oligosaccharides. The oligosaccharides are labeled with the number of [HexA, HexA, GlcN, Ac, SO 3 ] moieties present in each structure.

    Article Snippet: LMWH-1 and LMWH-4 were separated using a Luna HILIC column (2.0 × 150 mm2 , 200 Å, Phenomenex, Torrance, CA).

    Techniques: Hydrophilic Interaction Liquid Chromatography, Labeling

    Extracted ion chromatogram (XIC) of acylPG in corn roots obtained from ( a ) HILIC and ( b ) C30-RPLC, ( c ) HILIC-MS of total acylPG. Corresponding HRMS/MS spectrum of m/z 1029.76 obtained from ( d ) HILIC and ( e , f ) C30-RPLC. Structures of acylPG are inserted in the MS/MS spectra.

    Journal: Scientific Reports

    Article Title: Targeting Modified Lipids during Routine Lipidomics Analysis using HILIC and C30 Reverse Phase Liquid Chromatography coupled to Mass Spectrometry

    doi: 10.1038/s41598-019-41556-9

    Figure Lengend Snippet: Extracted ion chromatogram (XIC) of acylPG in corn roots obtained from ( a ) HILIC and ( b ) C30-RPLC, ( c ) HILIC-MS of total acylPG. Corresponding HRMS/MS spectrum of m/z 1029.76 obtained from ( d ) HILIC and ( e , f ) C30-RPLC. Structures of acylPG are inserted in the MS/MS spectra.

    Article Snippet: Analysis of the lipid mixture using HILIC or C30-RPLC The HILIC column (Luna 3 µm, 100 × 2 mm I.D., particle size: 3 µm, pore diameter: 200 Å) was purchased from Phenomenex (Torrance, CA, USA).

    Techniques: Hydrophilic Interaction Liquid Chromatography, Mass Spectrometry

    UHPLC-MS chromatogram in negative ion mode of the standard lipid mixture obtained after ( a ) HILIC and ( b ) C30-RPLC. ( c ) HILIC-MS spectrum of PI eluted at 22.54 min, ( d ) C30-RPLC-MS spectrum showing PI, PG and DMPE eluted at 19.85 min. ( e , f ) MS/MS spectra of PI 18:0/20:4 from HILIC and C30RPLC, respectively.

    Journal: Scientific Reports

    Article Title: Targeting Modified Lipids during Routine Lipidomics Analysis using HILIC and C30 Reverse Phase Liquid Chromatography coupled to Mass Spectrometry

    doi: 10.1038/s41598-019-41556-9

    Figure Lengend Snippet: UHPLC-MS chromatogram in negative ion mode of the standard lipid mixture obtained after ( a ) HILIC and ( b ) C30-RPLC. ( c ) HILIC-MS spectrum of PI eluted at 22.54 min, ( d ) C30-RPLC-MS spectrum showing PI, PG and DMPE eluted at 19.85 min. ( e , f ) MS/MS spectra of PI 18:0/20:4 from HILIC and C30RPLC, respectively.

    Article Snippet: Analysis of the lipid mixture using HILIC or C30-RPLC The HILIC column (Luna 3 µm, 100 × 2 mm I.D., particle size: 3 µm, pore diameter: 200 Å) was purchased from Phenomenex (Torrance, CA, USA).

    Techniques: Mass Spectrometry, Hydrophilic Interaction Liquid Chromatography