Structured Review

Phenomenex luna c18
HPLC overlay chromatogram of Bruguiera gymnorrhiza (BRG), n-butanol fraction of BRG and compound G. The HPLC fingerprint indicated the presence of compound G at retention time of 15.12 min. The analysis was carried out using Shimadzu high-performance liquid chromatography system attached with an ultraviolet detector and prepacked <t>C18</t> column (4.6 mm × 250 mm, 5 μm; Agilent Technologies). The separation was carried out using a gradient conc. of Solvent A (Water with 0.1% TFA) and Solvent B (Acetonitrile with 0.1% TFA). The total run time for the separation was 30 min (flow rate of 1 ml/min) at λ = 220 nm
Luna C18, supplied by Phenomenex, used in various techniques. Bioz Stars score: 86/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/luna c18/product/Phenomenex
Average 86 stars, based on 40 article reviews
Price from $9.99 to $1999.99
luna c18 - by Bioz Stars, 2022-08
86/100 stars

Images

1) Product Images from "5,7-dihydroxy-2-(3-hydroxy-4, 5-dimethoxy-phenyl)-chromen-4-one-a flavone from Bruguiera gymnorrhiza displaying anti-inflammatory properties"

Article Title: 5,7-dihydroxy-2-(3-hydroxy-4, 5-dimethoxy-phenyl)-chromen-4-one-a flavone from Bruguiera gymnorrhiza displaying anti-inflammatory properties

Journal: Indian Journal of Pharmacology

doi: 10.4103/0253-7613.182890

HPLC overlay chromatogram of Bruguiera gymnorrhiza (BRG), n-butanol fraction of BRG and compound G. The HPLC fingerprint indicated the presence of compound G at retention time of 15.12 min. The analysis was carried out using Shimadzu high-performance liquid chromatography system attached with an ultraviolet detector and prepacked C18 column (4.6 mm × 250 mm, 5 μm; Agilent Technologies). The separation was carried out using a gradient conc. of Solvent A (Water with 0.1% TFA) and Solvent B (Acetonitrile with 0.1% TFA). The total run time for the separation was 30 min (flow rate of 1 ml/min) at λ = 220 nm
Figure Legend Snippet: HPLC overlay chromatogram of Bruguiera gymnorrhiza (BRG), n-butanol fraction of BRG and compound G. The HPLC fingerprint indicated the presence of compound G at retention time of 15.12 min. The analysis was carried out using Shimadzu high-performance liquid chromatography system attached with an ultraviolet detector and prepacked C18 column (4.6 mm × 250 mm, 5 μm; Agilent Technologies). The separation was carried out using a gradient conc. of Solvent A (Water with 0.1% TFA) and Solvent B (Acetonitrile with 0.1% TFA). The total run time for the separation was 30 min (flow rate of 1 ml/min) at λ = 220 nm

Techniques Used: High Performance Liquid Chromatography, Flow Cytometry

2) Product Images from "Biosynthesis and Characterization of Zearalenone-14-Sulfate, Zearalenone-14-Glucoside and Zearalenone-16-Glucoside Using Common Fungal Strains"

Article Title: Biosynthesis and Characterization of Zearalenone-14-Sulfate, Zearalenone-14-Glucoside and Zearalenone-16-Glucoside Using Common Fungal Strains

Journal: Toxins

doi: 10.3390/toxins10030104

UV-chromatograms (λ = 265 nm) of the preparative separation (conducted on a Luna C18 reversed phase column; 250 mm × 21.2 mm; particle size 10 µm; pore size 100 Å) of extracted media from ( a ) Aspergillus oryzae NBRC 100959: Biosynthesis of zearalenone-14-sulfate (ZEN-14-S); ( b ) Rhizopus oryzae DSM 908: Biosynthesis of ZEN-14-glucoside (ZEN-14-G); ( c ) Rhizopus oligosporus CD: Biosynthesis of ZEN-16-glucoside (ZEN-16-G) with the byproducts ZEN, α-ZEL (α-zearalenol), α-ZEL-S (α-ZEL-sulfate), ZEL-G (ZEL glucoside).
Figure Legend Snippet: UV-chromatograms (λ = 265 nm) of the preparative separation (conducted on a Luna C18 reversed phase column; 250 mm × 21.2 mm; particle size 10 µm; pore size 100 Å) of extracted media from ( a ) Aspergillus oryzae NBRC 100959: Biosynthesis of zearalenone-14-sulfate (ZEN-14-S); ( b ) Rhizopus oryzae DSM 908: Biosynthesis of ZEN-14-glucoside (ZEN-14-G); ( c ) Rhizopus oligosporus CD: Biosynthesis of ZEN-16-glucoside (ZEN-16-G) with the byproducts ZEN, α-ZEL (α-zearalenol), α-ZEL-S (α-ZEL-sulfate), ZEL-G (ZEL glucoside).

Techniques Used:

3) Product Images from "High-Throughput Metabolic Profiling of Diverse Green Coffea arabica Beans Identified Tryptophan as a Universal Discrimination Factor for Immature Beans"

Article Title: High-Throughput Metabolic Profiling of Diverse Green Coffea arabica Beans Identified Tryptophan as a Universal Discrimination Factor for Immature Beans

Journal: PLoS ONE

doi: 10.1371/journal.pone.0070098

Development of a high-throughput analytical method for sample preparation and LC-MS measurement. First, green coffee beans were subjected to pulverization using a MultiBeads Shocker (Yasui Kikai, Japan), allowing us to process 18 samples in 20 seconds at once, while a conventional method using a mortar takes a few minutes for a single sample. Second, the extracted metabolites were subjected to LC-MS using a KINETEX C18 column, which allows a rapid (10 minute) separation for a single sample, which requires one-third less time than the conventional separation method.
Figure Legend Snippet: Development of a high-throughput analytical method for sample preparation and LC-MS measurement. First, green coffee beans were subjected to pulverization using a MultiBeads Shocker (Yasui Kikai, Japan), allowing us to process 18 samples in 20 seconds at once, while a conventional method using a mortar takes a few minutes for a single sample. Second, the extracted metabolites were subjected to LC-MS using a KINETEX C18 column, which allows a rapid (10 minute) separation for a single sample, which requires one-third less time than the conventional separation method.

Techniques Used: High Throughput Screening Assay, Sample Prep, Liquid Chromatography with Mass Spectroscopy

4) Product Images from "Nasal commensal Staphylococcus epidermidis counteracts influenza virus"

Article Title: Nasal commensal Staphylococcus epidermidis counteracts influenza virus

Journal: Scientific Reports

doi: 10.1038/srep27870

HPLC separation in combination with mass spectrometry for Embp identification. The proteins in the culture supernatants of S. epidermidis (ATCC12228) were separated by reversed-phase HPLC using a LUNA C18 5 μm column. ( a ) Twelve fractions were collected after HPLC separation. The HI activity of the eluted proteins (100 μl) in each fraction was tested using A/San Diego/1/09(H1N1 pdm09) as the virus antigen. ( b ) Tryptic digests of proteins in fraction 12 of the HLPC separation, which showed the greatest HI activity, were subjected to Nano-LC-LTQ MS/MS. A sequenced peptide (SINAYNKAIQSLETQITSAKDN) is shown and was identified as an internal peptide of Embp (Q8CP76). The m/z value of each “y” and “b” ion in collision-induced dissociation (CID) spectra is indicated.
Figure Legend Snippet: HPLC separation in combination with mass spectrometry for Embp identification. The proteins in the culture supernatants of S. epidermidis (ATCC12228) were separated by reversed-phase HPLC using a LUNA C18 5 μm column. ( a ) Twelve fractions were collected after HPLC separation. The HI activity of the eluted proteins (100 μl) in each fraction was tested using A/San Diego/1/09(H1N1 pdm09) as the virus antigen. ( b ) Tryptic digests of proteins in fraction 12 of the HLPC separation, which showed the greatest HI activity, were subjected to Nano-LC-LTQ MS/MS. A sequenced peptide (SINAYNKAIQSLETQITSAKDN) is shown and was identified as an internal peptide of Embp (Q8CP76). The m/z value of each “y” and “b” ion in collision-induced dissociation (CID) spectra is indicated.

Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry, Activity Assay

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Phenomenex c18 column luna
    Effect of iPLA 2 γ inhibitor, (R)-BEL, on SWCNT-induced pulmonary inflammation. ( A ) Production of pro- and anti-inflammatory cytokines in BAL of mice. Measurements were performed using Bio-Plex Pro Mouse Cytokine 23-Plex Immunoassay kit, composed of a combination of pro- and anti-inflammatory cytokines. ( B ) Representative images of lung sections from mice on day 1 post exposure to SWCNT or R-BEL alone, SWCNT/R-BEL together and control group (neutrophils indicated by arrows). ( C ) Average neutrophil counts per whole lung sections from 3 mice per group. ( D ) Tissue damage and fibrogenic response in BAL of mice. Pulmonary tissue damage was measured as protein in BAL of mice on day 1 after exposure to SWCNT (40 μ g/mouse; a). Fibrogenic response was assessed on day 7 post exposure to SWCNT (40 μ g/mouse) by the levels of collagen measured in the lung of mice (b). ( E ) Production of pro-inflammatory lipid mediators, LTB 4 (a), and HXB 3 (b) in lung of C57B6J mice. Structural formulas of LTB 4 and HXB 3 (inserts). ( F ) (R)-BEL treatment reversed the increases in PNLP8 expression (green) and cell death (TUNEL(magenta) positive nuclei, arrows) induced by SWCNT exposure in mouse lungs (a), % PNLP8 positive cells relative to total cell number (b), % TUNEL positive cells relative to total cell number (c). ( G ) MS spectrum of CL obtained from control mouse lung (a). Base peak chromatograms of CL molecular species with m / z 1475.992 from lung of control mouse and mouse exposed to SWCNT in the absence and in the presence of (R)-BEL on day 1 after treatment (b). MS 2 spectrum of CL molecular species with m / z 1475.992 containing arachidonic acid (C20:4), <t>CL-C18:1/C18:2/C18:1/C20:4,</t> obtained from control mouse lung (c). All data are mean ± SD ( n = 4–5 mice per group). * P
    C18 Column Luna, supplied by Phenomenex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c18 column luna/product/Phenomenex
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    c18 column luna - by Bioz Stars, 2022-08
    86/100 stars
      Buy from Supplier

    86
    Phenomenex luna c18
    HPLC overlay chromatogram of Bruguiera gymnorrhiza (BRG), n-butanol fraction of BRG and compound G. The HPLC fingerprint indicated the presence of compound G at retention time of 15.12 min. The analysis was carried out using Shimadzu high-performance liquid chromatography system attached with an ultraviolet detector and prepacked <t>C18</t> column (4.6 mm × 250 mm, 5 μm; Agilent Technologies). The separation was carried out using a gradient conc. of Solvent A (Water with 0.1% TFA) and Solvent B (Acetonitrile with 0.1% TFA). The total run time for the separation was 30 min (flow rate of 1 ml/min) at λ = 220 nm
    Luna C18, supplied by Phenomenex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luna c18/product/Phenomenex
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    luna c18 - by Bioz Stars, 2022-08
    86/100 stars
      Buy from Supplier

    86
    Phenomenex c18
    Chromatogram of concentrated methanol extract of ( a ) purified beeswax, ( b ) raw beeswax, and ( c ) green propolis. Chromatographic conditions: <t>C18</t> column with a mobile phase composed of (A) water containing 0.4% formic acid, 5% methanol, and 2% isopropanol, and (B) acetonitrile and 2% isopropanol. The flow was 1 mL/min in the concentration gradient, using 20% B for 3 min, 20–25% B for 3–4 min, 25% B for 4–15 min, 25–45% B for 15–20 min, 45% B for 20–40 min, 45–60% B for 40–45 min, 60–80% B for 45–68.83 min, 80–20% B for 68.86–70 min, and 20% B for 70–80 min. An injection volume of 15 µL, oven temperature of 30 °C, and wavelength of 300 nm were used.
    C18, supplied by Phenomenex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c18/product/Phenomenex
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    c18 - by Bioz Stars, 2022-08
    86/100 stars
      Buy from Supplier

    86
    Phenomenex ps c18
    TIC of 6 analytes under four columns ( a ) Phenomenex Luna Omega 3 µm <t>PS</t> <t>C18</t> 100 Å; ( b ) Agilent Eclipse Plus C18; ( c ) Agilent Poroshell 120 EC-C18; ( d ) Agilent ZORBOX SB-C18. (FK-506 and PIM at 100 ng·mL −1 , RAPA and EVER at 500 ng·mL −1 , MMF at 10 ng·mL −1 and MPA at 1000 ng·mL −1 ).
    Ps C18, supplied by Phenomenex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ps c18/product/Phenomenex
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ps c18 - by Bioz Stars, 2022-08
    86/100 stars
      Buy from Supplier

    Image Search Results


    Effect of iPLA 2 γ inhibitor, (R)-BEL, on SWCNT-induced pulmonary inflammation. ( A ) Production of pro- and anti-inflammatory cytokines in BAL of mice. Measurements were performed using Bio-Plex Pro Mouse Cytokine 23-Plex Immunoassay kit, composed of a combination of pro- and anti-inflammatory cytokines. ( B ) Representative images of lung sections from mice on day 1 post exposure to SWCNT or R-BEL alone, SWCNT/R-BEL together and control group (neutrophils indicated by arrows). ( C ) Average neutrophil counts per whole lung sections from 3 mice per group. ( D ) Tissue damage and fibrogenic response in BAL of mice. Pulmonary tissue damage was measured as protein in BAL of mice on day 1 after exposure to SWCNT (40 μ g/mouse; a). Fibrogenic response was assessed on day 7 post exposure to SWCNT (40 μ g/mouse) by the levels of collagen measured in the lung of mice (b). ( E ) Production of pro-inflammatory lipid mediators, LTB 4 (a), and HXB 3 (b) in lung of C57B6J mice. Structural formulas of LTB 4 and HXB 3 (inserts). ( F ) (R)-BEL treatment reversed the increases in PNLP8 expression (green) and cell death (TUNEL(magenta) positive nuclei, arrows) induced by SWCNT exposure in mouse lungs (a), % PNLP8 positive cells relative to total cell number (b), % TUNEL positive cells relative to total cell number (c). ( G ) MS spectrum of CL obtained from control mouse lung (a). Base peak chromatograms of CL molecular species with m / z 1475.992 from lung of control mouse and mouse exposed to SWCNT in the absence and in the presence of (R)-BEL on day 1 after treatment (b). MS 2 spectrum of CL molecular species with m / z 1475.992 containing arachidonic acid (C20:4), CL-C18:1/C18:2/C18:1/C20:4, obtained from control mouse lung (c). All data are mean ± SD ( n = 4–5 mice per group). * P

    Journal: Journal of leukocyte biology

    Article Title: Redox (phospho)lipidomics of signaling in inflammation and programmed cell death

    doi: 10.1002/JLB.3MIR0119-004RR

    Figure Lengend Snippet: Effect of iPLA 2 γ inhibitor, (R)-BEL, on SWCNT-induced pulmonary inflammation. ( A ) Production of pro- and anti-inflammatory cytokines in BAL of mice. Measurements were performed using Bio-Plex Pro Mouse Cytokine 23-Plex Immunoassay kit, composed of a combination of pro- and anti-inflammatory cytokines. ( B ) Representative images of lung sections from mice on day 1 post exposure to SWCNT or R-BEL alone, SWCNT/R-BEL together and control group (neutrophils indicated by arrows). ( C ) Average neutrophil counts per whole lung sections from 3 mice per group. ( D ) Tissue damage and fibrogenic response in BAL of mice. Pulmonary tissue damage was measured as protein in BAL of mice on day 1 after exposure to SWCNT (40 μ g/mouse; a). Fibrogenic response was assessed on day 7 post exposure to SWCNT (40 μ g/mouse) by the levels of collagen measured in the lung of mice (b). ( E ) Production of pro-inflammatory lipid mediators, LTB 4 (a), and HXB 3 (b) in lung of C57B6J mice. Structural formulas of LTB 4 and HXB 3 (inserts). ( F ) (R)-BEL treatment reversed the increases in PNLP8 expression (green) and cell death (TUNEL(magenta) positive nuclei, arrows) induced by SWCNT exposure in mouse lungs (a), % PNLP8 positive cells relative to total cell number (b), % TUNEL positive cells relative to total cell number (c). ( G ) MS spectrum of CL obtained from control mouse lung (a). Base peak chromatograms of CL molecular species with m / z 1475.992 from lung of control mouse and mouse exposed to SWCNT in the absence and in the presence of (R)-BEL on day 1 after treatment (b). MS 2 spectrum of CL molecular species with m / z 1475.992 containing arachidonic acid (C20:4), CL-C18:1/C18:2/C18:1/C20:4, obtained from control mouse lung (c). All data are mean ± SD ( n = 4–5 mice per group). * P

    Article Snippet: Cardiolipins were separated on a C18 column Luna (2) 3 μ m, 100 Å, 150 × 1 mm (Phenomenex) at a flow rate of 0.050 mL/min on a Dionex Ultimate 3000 HPLC system.

    Techniques: Mouse Assay, Expressing, TUNEL Assay, Mass Spectrometry

    HPLC overlay chromatogram of Bruguiera gymnorrhiza (BRG), n-butanol fraction of BRG and compound G. The HPLC fingerprint indicated the presence of compound G at retention time of 15.12 min. The analysis was carried out using Shimadzu high-performance liquid chromatography system attached with an ultraviolet detector and prepacked C18 column (4.6 mm × 250 mm, 5 μm; Agilent Technologies). The separation was carried out using a gradient conc. of Solvent A (Water with 0.1% TFA) and Solvent B (Acetonitrile with 0.1% TFA). The total run time for the separation was 30 min (flow rate of 1 ml/min) at λ = 220 nm

    Journal: Indian Journal of Pharmacology

    Article Title: 5,7-dihydroxy-2-(3-hydroxy-4, 5-dimethoxy-phenyl)-chromen-4-one-a flavone from Bruguiera gymnorrhiza displaying anti-inflammatory properties

    doi: 10.4103/0253-7613.182890

    Figure Lengend Snippet: HPLC overlay chromatogram of Bruguiera gymnorrhiza (BRG), n-butanol fraction of BRG and compound G. The HPLC fingerprint indicated the presence of compound G at retention time of 15.12 min. The analysis was carried out using Shimadzu high-performance liquid chromatography system attached with an ultraviolet detector and prepacked C18 column (4.6 mm × 250 mm, 5 μm; Agilent Technologies). The separation was carried out using a gradient conc. of Solvent A (Water with 0.1% TFA) and Solvent B (Acetonitrile with 0.1% TFA). The total run time for the separation was 30 min (flow rate of 1 ml/min) at λ = 220 nm

    Article Snippet: A Luna C18 (30 mm × 2.0 mm and 5 µm particle size, Phenomenex, USA) column was used in liquid chromatography–mass spectrometry (LC/MS and LC/MS/MSs) analysis.

    Techniques: High Performance Liquid Chromatography, Flow Cytometry

    Chromatogram of concentrated methanol extract of ( a ) purified beeswax, ( b ) raw beeswax, and ( c ) green propolis. Chromatographic conditions: C18 column with a mobile phase composed of (A) water containing 0.4% formic acid, 5% methanol, and 2% isopropanol, and (B) acetonitrile and 2% isopropanol. The flow was 1 mL/min in the concentration gradient, using 20% B for 3 min, 20–25% B for 3–4 min, 25% B for 4–15 min, 25–45% B for 15–20 min, 45% B for 20–40 min, 45–60% B for 40–45 min, 60–80% B for 45–68.83 min, 80–20% B for 68.86–70 min, and 20% B for 70–80 min. An injection volume of 15 µL, oven temperature of 30 °C, and wavelength of 300 nm were used.

    Journal: Pharmaceutics

    Article Title: A New Approach to Atopic Dermatitis Control with Low-Concentration Propolis-Loaded Cold Cream

    doi: 10.3390/pharmaceutics13091346

    Figure Lengend Snippet: Chromatogram of concentrated methanol extract of ( a ) purified beeswax, ( b ) raw beeswax, and ( c ) green propolis. Chromatographic conditions: C18 column with a mobile phase composed of (A) water containing 0.4% formic acid, 5% methanol, and 2% isopropanol, and (B) acetonitrile and 2% isopropanol. The flow was 1 mL/min in the concentration gradient, using 20% B for 3 min, 20–25% B for 3–4 min, 25% B for 4–15 min, 25–45% B for 15–20 min, 45% B for 20–40 min, 45–60% B for 40–45 min, 60–80% B for 45–68.83 min, 80–20% B for 68.86–70 min, and 20% B for 70–80 min. An injection volume of 15 µL, oven temperature of 30 °C, and wavelength of 300 nm were used.

    Article Snippet: The column used was a C18 (Phenomenex LUNA, Torrance, CA, USA) of 250 mm × 4.6 × 5 μm with a precolumn C18 of 4 mm × 3.0 mm.

    Techniques: Purification, Concentration Assay, Injection

    TIC of 6 analytes under four columns ( a ) Phenomenex Luna Omega 3 µm PS C18 100 Å; ( b ) Agilent Eclipse Plus C18; ( c ) Agilent Poroshell 120 EC-C18; ( d ) Agilent ZORBOX SB-C18. (FK-506 and PIM at 100 ng·mL −1 , RAPA and EVER at 500 ng·mL −1 , MMF at 10 ng·mL −1 and MPA at 1000 ng·mL −1 ).

    Journal: Molecules

    Article Title: Simultaneous Determination of Six Immunosuppressants in Human Whole Blood by HPLC-MS/MS Using a Modified QuEChERS Method

    doi: 10.3390/molecules27134087

    Figure Lengend Snippet: TIC of 6 analytes under four columns ( a ) Phenomenex Luna Omega 3 µm PS C18 100 Å; ( b ) Agilent Eclipse Plus C18; ( c ) Agilent Poroshell 120 EC-C18; ( d ) Agilent ZORBOX SB-C18. (FK-506 and PIM at 100 ng·mL −1 , RAPA and EVER at 500 ng·mL −1 , MMF at 10 ng·mL −1 and MPA at 1000 ng·mL −1 ).

    Article Snippet: The separation performances of four chromatographic columns were also assessed in this study. (1) Phenomenex Luna Omega 3 µm PS C18 100 Å (2.1 mm × 150 mm); (2) Agilent Eclipse Plus C18 (3.0 × 100 mm, 1.8 μm); (3) Agilent Poroshell 120 EC-C18 (3.0 × 50 mm, 2.7 μm), and (4) Agilent ZORBOX SB-C18 (4.6 × 150 mm, 5 μm).

    Techniques: