Journal: International Journal of Molecular Sciences

Article Title: Dual Modulator of ASIC Channels and GABA A Receptors from Thyme Alters Fear-Related Hippocampal Activity

doi: 10.3390/ijms241713148

Figure Lengend Snippet: ( A ) Chemical structure of sevanol. The asterisk designates the chiral center in which sevanol and isosevanol differ in configuration. ( B , C ) Separation of diastereomers, 1—isosevanol, 2—sevanol, on a reversed-phase column Luna C 18 (2) (250 × 10 mm) by elution in a linear gradient of acetonitrile with 0.1% TFA (50 µg of the mixture, panel B) and by isocratic elution in 10% acetonitrile with 0.1% TFA (300 µg of the mixture, panel C).

Article Snippet: The purification was performedby high-performance liquid chromatography (HPLC) using a reversed-phase column Luna C 18 (2) (250 × 10 mm) (Phenomenex, Torrance, CA, USA) with isocratic elution by 10% acetonitrile with 0.1% trifluoroacetic acid (TFA) or in a slow linear gradient of acetonitrile concentration with 0.1% TFA.

Techniques: