Structured Review

Phenomenex luna c18 column
Effects of Guiera senegalensis hydroethanolic extract (GS: 1 µg/L, 4 µg/L and 8 µg/L) on Sco-induced increasing in the activity of the acetylcholinesterase (AChE). Each <t>column</t> represents mean ± S.E.M. of 10 zebrafish. For Tukey’s post hoc analyses: *** p
Luna C18 Column, supplied by Phenomenex, used in various techniques. Bioz Stars score: 86/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Neuroprotective Potential of Guiera senegalensis (Combretaceae) Leaf Hydroethanolic Extract against Cholinergic System Dysfunctions and Oxidative Stress in Scopolamine-Induced Cognitive Impairment in Zebrafish (Danio rerio)"

Article Title: Neuroprotective Potential of Guiera senegalensis (Combretaceae) Leaf Hydroethanolic Extract against Cholinergic System Dysfunctions and Oxidative Stress in Scopolamine-Induced Cognitive Impairment in Zebrafish (Danio rerio)

Journal: Plants

doi: 10.3390/plants11091149

Effects of Guiera senegalensis hydroethanolic extract (GS: 1 µg/L, 4 µg/L and 8 µg/L) on Sco-induced increasing in the activity of the acetylcholinesterase (AChE). Each column represents mean ± S.E.M. of 10 zebrafish. For Tukey’s post hoc analyses: *** p
Figure Legend Snippet: Effects of Guiera senegalensis hydroethanolic extract (GS: 1 µg/L, 4 µg/L and 8 µg/L) on Sco-induced increasing in the activity of the acetylcholinesterase (AChE). Each column represents mean ± S.E.M. of 10 zebrafish. For Tukey’s post hoc analyses: *** p

Techniques Used: Activity Assay

2) Product Images from "Plasticity, dynamics, and inhibition of emerging tetracycline-resistance enzymes"

Article Title: Plasticity, dynamics, and inhibition of emerging tetracycline-resistance enzymes

Journal: Nature chemical biology

doi: 10.1038/nchembio.2376

Anhydrotetracycline prevents enzymatic tetracycline degradation, functionally rescuing tetracycline antibiotic activity (a) Tetracycline (TC) is degraded by Tet(56) in vitro HPLC chromatograms show in vitro reactions with UV detection at 363 nm and separation on a C18 column. (b) TC degradation is attenuated by the addition of an excess of aTC. (c) Dose-dependent inhibition of Tet(50,51,56) activity by anhydrotetracycline. Velocity is determined by measuring tetracycline consumption via change in absorbance at 400 nm. Data are represented as mean ± s.d. of three technical replicates. (d) Dose-response curve showing effect of aTC on sensitivity of Tet(56)-expressing E. coli to TC in liquid culture. Data are represented as mean ± s.e.m. of three technical replicates. (e) TC and aTC synergistically inhibit growth of E. coli expressing Tet(56), FICI = 0.1875. Points show minimum inhibitory concentrations of two drugs in combination. Dashed line indicates the theoretical concentration of additive drug interaction. Data represented as mean ± s.e.m. of three technical replicates.
Figure Legend Snippet: Anhydrotetracycline prevents enzymatic tetracycline degradation, functionally rescuing tetracycline antibiotic activity (a) Tetracycline (TC) is degraded by Tet(56) in vitro HPLC chromatograms show in vitro reactions with UV detection at 363 nm and separation on a C18 column. (b) TC degradation is attenuated by the addition of an excess of aTC. (c) Dose-dependent inhibition of Tet(50,51,56) activity by anhydrotetracycline. Velocity is determined by measuring tetracycline consumption via change in absorbance at 400 nm. Data are represented as mean ± s.d. of three technical replicates. (d) Dose-response curve showing effect of aTC on sensitivity of Tet(56)-expressing E. coli to TC in liquid culture. Data are represented as mean ± s.e.m. of three technical replicates. (e) TC and aTC synergistically inhibit growth of E. coli expressing Tet(56), FICI = 0.1875. Points show minimum inhibitory concentrations of two drugs in combination. Dashed line indicates the theoretical concentration of additive drug interaction. Data represented as mean ± s.e.m. of three technical replicates.

Techniques Used: Activity Assay, In Vitro, High Performance Liquid Chromatography, Inhibition, Expressing, Concentration Assay

3) Product Images from "Flavones from Erythrina falcata are modulators of fear memory"

Article Title: Flavones from Erythrina falcata are modulators of fear memory

Journal: BMC Complementary and Alternative Medicine

doi: 10.1186/1472-6882-14-288

MS 2 spectra of deprotonated molecules of crude extracts (CEs) from the roots of Erythrina falcata using a C18 Luna column as follows: (A) vicenin-2 [M-H] − at m/z 593 (1), (B) vicenin-1 [M-H] − at m/z 563 (2), (C) vitexin [M-H] − at m/z 431 (3), (D) isovitexin [M-H] − at m/z 431 (4), (E) 6-C-glycoside diosmetin [M-H] − at m/z 461 (5) and (F) apigenin [M-H] − at m/z 269 (6). Fragment ions used as structural markers are indicated by arrows.
Figure Legend Snippet: MS 2 spectra of deprotonated molecules of crude extracts (CEs) from the roots of Erythrina falcata using a C18 Luna column as follows: (A) vicenin-2 [M-H] − at m/z 593 (1), (B) vicenin-1 [M-H] − at m/z 563 (2), (C) vitexin [M-H] − at m/z 431 (3), (D) isovitexin [M-H] − at m/z 431 (4), (E) 6-C-glycoside diosmetin [M-H] − at m/z 461 (5) and (F) apigenin [M-H] − at m/z 269 (6). Fragment ions used as structural markers are indicated by arrows.

Techniques Used: Mass Spectrometry

HPLC-DAD-ESI/MS n. analysis of crude extracts (CEs) from the roots of Erythrina falcata using a C18 Luna column (A, B). The chromatogram was recorded at 254 nm (A) and the TIC was in negative mode (B) . The UV spectra of compounds 1–6 (C) .
Figure Legend Snippet: HPLC-DAD-ESI/MS n. analysis of crude extracts (CEs) from the roots of Erythrina falcata using a C18 Luna column (A, B). The chromatogram was recorded at 254 nm (A) and the TIC was in negative mode (B) . The UV spectra of compounds 1–6 (C) .

Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry

4) Product Images from "Metabolic engineering of folate and its precursors in Mexican common bean (Phaseolus vulgaris L.)"

Article Title: Metabolic engineering of folate and its precursors in Mexican common bean (Phaseolus vulgaris L.)

Journal: Plant Biotechnology Journal

doi: 10.1111/pbi.12561

Pteridine profiles in Pinto bean seeds. Oxidized pteridines from Pinto Café wild‐type seeds (Panel a). Profile of pteridine standards added to a Pinto Café extract (Panel b). Pteridine profile of C18 engineered line (Panel c). CPt, 6‐carboxypterin (1); NPt, neopterin (2); MPt, monapterin (3); NPt‐G, neopterin glycoside (4); Pt, pterin (5); PtAl, pterin 6‐aldehyde (6); HMPt, 6‐hydroxymethylpterin (7); XPt, xanthopterin (not detected in seed extract) (8); unknown peak (?).
Figure Legend Snippet: Pteridine profiles in Pinto bean seeds. Oxidized pteridines from Pinto Café wild‐type seeds (Panel a). Profile of pteridine standards added to a Pinto Café extract (Panel b). Pteridine profile of C18 engineered line (Panel c). CPt, 6‐carboxypterin (1); NPt, neopterin (2); MPt, monapterin (3); NPt‐G, neopterin glycoside (4); Pt, pterin (5); PtAl, pterin 6‐aldehyde (6); HMPt, 6‐hydroxymethylpterin (7); XPt, xanthopterin (not detected in seed extract) (8); unknown peak (?).

Techniques Used: Cycling Probe Technology

5) Product Images from "A Drug Delivery Strategy: Binding Enkephalin to Asialoglycoprotein Receptor by Enzymatic Galactosylation"

Article Title: A Drug Delivery Strategy: Binding Enkephalin to Asialoglycoprotein Receptor by Enzymatic Galactosylation

Journal: PLoS ONE

doi: 10.1371/journal.pone.0095024

Enzymatic transformation of Lac-Enk to Gal-Lac-Enk. A) RP-HPLC analysis of one-pot reaction for Lac-Enk with UDP-Glc and galactose epimerase. From top to bottom: substrate (Lac-Enk) only; positive control – reaction mixture containing UDP-Gal and LgtC; negative control – reaction mixture containing UDP-Glc and LgtC; reaction mixture containing UDP-Glc, LgtC and galactose epimerase. All reactions were conducted in 50 mM HEPES (pH 7), 10 mM MnCl 2 buffer at 37°C. RP-HPLC was conducted on an Alltima C18 column using a gradient of 0–90% solvent B, t R = 17.2 min (substrate, Lac-Enk) and t R = 16.8 min (product, Gal-Lac-Enk). B) ES-MS analysis of the acceptor, Lac-Enk m/z (C 44 H 63 N 7 O 18 , 977.4) m/z 978.5 [M+H] + (calcd. 978.4). C) ES-MS analysis of the product, Gal-Lac-Enk (C 52 H 76 N 8 O 23 , 1139.5) m/z 1140.5 [M+H] + (calcd. 1140.5).
Figure Legend Snippet: Enzymatic transformation of Lac-Enk to Gal-Lac-Enk. A) RP-HPLC analysis of one-pot reaction for Lac-Enk with UDP-Glc and galactose epimerase. From top to bottom: substrate (Lac-Enk) only; positive control – reaction mixture containing UDP-Gal and LgtC; negative control – reaction mixture containing UDP-Glc and LgtC; reaction mixture containing UDP-Glc, LgtC and galactose epimerase. All reactions were conducted in 50 mM HEPES (pH 7), 10 mM MnCl 2 buffer at 37°C. RP-HPLC was conducted on an Alltima C18 column using a gradient of 0–90% solvent B, t R = 17.2 min (substrate, Lac-Enk) and t R = 16.8 min (product, Gal-Lac-Enk). B) ES-MS analysis of the acceptor, Lac-Enk m/z (C 44 H 63 N 7 O 18 , 977.4) m/z 978.5 [M+H] + (calcd. 978.4). C) ES-MS analysis of the product, Gal-Lac-Enk (C 52 H 76 N 8 O 23 , 1139.5) m/z 1140.5 [M+H] + (calcd. 1140.5).

Techniques Used: Transformation Assay, High Performance Liquid Chromatography, Positive Control, Negative Control

Stability profiles of enkephalin and carbohydrate-derived enkephalins. A) Plasma stability assay: the concentration of compounds in plasma was determined at various time points using RP-HPLC on a C18 Vydac column, gradient of 20–35% (enkephalin) or 0–70% solvent B (glycosylated enkephalins) over 30 min. B) Caco-2 cell stability assay: The concentration of compound in Caco-2 cell homogenates at various time points was quantified using LC-MS. Data presented is the mean ± S.D. (n = 3).
Figure Legend Snippet: Stability profiles of enkephalin and carbohydrate-derived enkephalins. A) Plasma stability assay: the concentration of compounds in plasma was determined at various time points using RP-HPLC on a C18 Vydac column, gradient of 20–35% (enkephalin) or 0–70% solvent B (glycosylated enkephalins) over 30 min. B) Caco-2 cell stability assay: The concentration of compound in Caco-2 cell homogenates at various time points was quantified using LC-MS. Data presented is the mean ± S.D. (n = 3).

Techniques Used: Derivative Assay, Stability Assay, Concentration Assay, High Performance Liquid Chromatography, Liquid Chromatography with Mass Spectroscopy

6) Product Images from "A Drug Delivery Strategy: Binding Enkephalin to Asialoglycoprotein Receptor by Enzymatic Galactosylation"

Article Title: A Drug Delivery Strategy: Binding Enkephalin to Asialoglycoprotein Receptor by Enzymatic Galactosylation

Journal: PLoS ONE

doi: 10.1371/journal.pone.0095024

Enzymatic transformation of Lac-Enk to Gal-Lac-Enk. A) RP-HPLC analysis of one-pot reaction for Lac-Enk with UDP-Glc and galactose epimerase. From top to bottom: substrate (Lac-Enk) only; positive control – reaction mixture containing UDP-Gal and LgtC; negative control – reaction mixture containing UDP-Glc and LgtC; reaction mixture containing UDP-Glc, LgtC and galactose epimerase. All reactions were conducted in 50 mM HEPES (pH 7), 10 mM MnCl 2 buffer at 37°C. RP-HPLC was conducted on an Alltima C18 column using a gradient of 0–90% solvent B, t R = 17.2 min (substrate, Lac-Enk) and t R = 16.8 min (product, Gal-Lac-Enk). B) ES-MS analysis of the acceptor, Lac-Enk m/z (C 44 H 63 N 7 O 18 , 977.4) m/z 978.5 [M+H] + (calcd. 978.4). C) ES-MS analysis of the product, Gal-Lac-Enk (C 52 H 76 N 8 O 23 , 1139.5) m/z 1140.5 [M+H] + (calcd. 1140.5).
Figure Legend Snippet: Enzymatic transformation of Lac-Enk to Gal-Lac-Enk. A) RP-HPLC analysis of one-pot reaction for Lac-Enk with UDP-Glc and galactose epimerase. From top to bottom: substrate (Lac-Enk) only; positive control – reaction mixture containing UDP-Gal and LgtC; negative control – reaction mixture containing UDP-Glc and LgtC; reaction mixture containing UDP-Glc, LgtC and galactose epimerase. All reactions were conducted in 50 mM HEPES (pH 7), 10 mM MnCl 2 buffer at 37°C. RP-HPLC was conducted on an Alltima C18 column using a gradient of 0–90% solvent B, t R = 17.2 min (substrate, Lac-Enk) and t R = 16.8 min (product, Gal-Lac-Enk). B) ES-MS analysis of the acceptor, Lac-Enk m/z (C 44 H 63 N 7 O 18 , 977.4) m/z 978.5 [M+H] + (calcd. 978.4). C) ES-MS analysis of the product, Gal-Lac-Enk (C 52 H 76 N 8 O 23 , 1139.5) m/z 1140.5 [M+H] + (calcd. 1140.5).

Techniques Used: Transformation Assay, High Performance Liquid Chromatography, Positive Control, Negative Control

Stability profiles of enkephalin and carbohydrate-derived enkephalins. A) Plasma stability assay: the concentration of compounds in plasma was determined at various time points using RP-HPLC on a C18 Vydac column, gradient of 20–35% (enkephalin) or 0–70% solvent B (glycosylated enkephalins) over 30 min. B) Caco-2 cell stability assay: The concentration of compound in Caco-2 cell homogenates at various time points was quantified using LC-MS. Data presented is the mean ± S.D. (n = 3).
Figure Legend Snippet: Stability profiles of enkephalin and carbohydrate-derived enkephalins. A) Plasma stability assay: the concentration of compounds in plasma was determined at various time points using RP-HPLC on a C18 Vydac column, gradient of 20–35% (enkephalin) or 0–70% solvent B (glycosylated enkephalins) over 30 min. B) Caco-2 cell stability assay: The concentration of compound in Caco-2 cell homogenates at various time points was quantified using LC-MS. Data presented is the mean ± S.D. (n = 3).

Techniques Used: Derivative Assay, Stability Assay, Concentration Assay, High Performance Liquid Chromatography, Liquid Chromatography with Mass Spectroscopy

7) Product Images from "Aqueous Leaf Extract of Jatropha mollissima (Pohl) Bail Decreases Local Effects Induced by Bothropic Venom"

Article Title: Aqueous Leaf Extract of Jatropha mollissima (Pohl) Bail Decreases Local Effects Induced by Bothropic Venom

Journal: BioMed Research International

doi: 10.1155/2016/6101742

High Performance Liquid Chromatography of the aqueous extract of J. mollissima. SP: Phenomenex Luna C18 column (250 × 4.6 mm, 5 μ m); MP: ACN gradient: acetic acid 0.3%; flow rate: 0.7 mL/min; detection: 340 nm. Four compounds were identified as isoorientin (peak 3), orientin (peak 4), vitexin (peak 5), and isovitexin (peak 6).
Figure Legend Snippet: High Performance Liquid Chromatography of the aqueous extract of J. mollissima. SP: Phenomenex Luna C18 column (250 × 4.6 mm, 5 μ m); MP: ACN gradient: acetic acid 0.3%; flow rate: 0.7 mL/min; detection: 340 nm. Four compounds were identified as isoorientin (peak 3), orientin (peak 4), vitexin (peak 5), and isovitexin (peak 6).

Techniques Used: High Performance Liquid Chromatography, Flow Cytometry

8) Product Images from "Flavones from Erythrina falcata are modulators of fear memory"

Article Title: Flavones from Erythrina falcata are modulators of fear memory

Journal: BMC Complementary and Alternative Medicine

doi: 10.1186/1472-6882-14-288

MS 2 spectra of deprotonated molecules of crude extracts (CEs) from the roots of Erythrina falcata using a C18 Luna column as follows: (A) vicenin-2 [M-H] − at m/z 593 (1), (B) vicenin-1 [M-H] − at m/z 563 (2), (C) vitexin [M-H] − at m/z 431 (3), (D) isovitexin [M-H] − at m/z 431 (4), (E) 6-C-glycoside diosmetin [M-H] − at m/z 461 (5) and (F) apigenin [M-H] − at m/z 269 (6). Fragment ions used as structural markers are indicated by arrows.
Figure Legend Snippet: MS 2 spectra of deprotonated molecules of crude extracts (CEs) from the roots of Erythrina falcata using a C18 Luna column as follows: (A) vicenin-2 [M-H] − at m/z 593 (1), (B) vicenin-1 [M-H] − at m/z 563 (2), (C) vitexin [M-H] − at m/z 431 (3), (D) isovitexin [M-H] − at m/z 431 (4), (E) 6-C-glycoside diosmetin [M-H] − at m/z 461 (5) and (F) apigenin [M-H] − at m/z 269 (6). Fragment ions used as structural markers are indicated by arrows.

Techniques Used: Mass Spectrometry

HPLC-DAD-ESI/MS n. analysis of crude extracts (CEs) from the roots of Erythrina falcata using a C18 Luna column (A, B). The chromatogram was recorded at 254 nm (A) and the TIC was in negative mode (B) . The UV spectra of compounds 1–6 (C) .
Figure Legend Snippet: HPLC-DAD-ESI/MS n. analysis of crude extracts (CEs) from the roots of Erythrina falcata using a C18 Luna column (A, B). The chromatogram was recorded at 254 nm (A) and the TIC was in negative mode (B) . The UV spectra of compounds 1–6 (C) .

Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry

9) Product Images from "Synthesis and Chemical and Biological Comparison of Nitroxyl and Nitric Oxide Releasing Diazeniumdiolate-based Aspirin Derivatives"

Article Title: Synthesis and Chemical and Biological Comparison of Nitroxyl and Nitric Oxide Releasing Diazeniumdiolate-based Aspirin Derivatives

Journal: Journal of medicinal chemistry

doi: 10.1021/jm400196q

HPLC analysis of guinea pig serum induced hydrolysis of ( A ) DEA/NO-aspirin or ( B ) IPA/NO-aspirin (100 μ M) in 100 mM phosphate buffer containing 50 μ M DTPA at pH 7.4 and 37°C. Authentic standards eluted from a Phenomenex Luna C18
Figure Legend Snippet: HPLC analysis of guinea pig serum induced hydrolysis of ( A ) DEA/NO-aspirin or ( B ) IPA/NO-aspirin (100 μ M) in 100 mM phosphate buffer containing 50 μ M DTPA at pH 7.4 and 37°C. Authentic standards eluted from a Phenomenex Luna C18

Techniques Used: High Performance Liquid Chromatography, Indirect Immunoperoxidase Assay

10) Product Images from "Production of 22-Hydroxy Metabolites of Vitamin D3 by Cytochrome P450scc (CYP11A1) and Analysis of Their Biological Activities on Skin Cells S⃞"

Article Title: Production of 22-Hydroxy Metabolites of Vitamin D3 by Cytochrome P450scc (CYP11A1) and Analysis of Their Biological Activities on Skin Cells S⃞

Journal: Drug Metabolism and Disposition

doi: 10.1124/dmd.111.040071

HPLC analysis of the products of vitamin D 3 metabolism by cytochrome P450scc. Incubations were carried out as described in the legend to . A, HPLC was performed on a C18 GraceSmart column (15 cm × 4.6 mm, particle size 5 μm). The
Figure Legend Snippet: HPLC analysis of the products of vitamin D 3 metabolism by cytochrome P450scc. Incubations were carried out as described in the legend to . A, HPLC was performed on a C18 GraceSmart column (15 cm × 4.6 mm, particle size 5 μm). The

Techniques Used: High Performance Liquid Chromatography

11) Product Images from "Serum Sphingolipid Variations Associate with Hepatic Decompensation and Survival in Patients with Cirrhosis"

Article Title: Serum Sphingolipid Variations Associate with Hepatic Decompensation and Survival in Patients with Cirrhosis

Journal: PLoS ONE

doi: 10.1371/journal.pone.0138130

Severity of cirrhosis associates with a decrease of serum Cer levels. C18, C20, C24 and C24:1Cer are significantly decreased with progression of cirrhosis, defined by the Child-Pugh score (p
Figure Legend Snippet: Severity of cirrhosis associates with a decrease of serum Cer levels. C18, C20, C24 and C24:1Cer are significantly decreased with progression of cirrhosis, defined by the Child-Pugh score (p

Techniques Used:

12) Product Images from "An efficient new method for the synthesis of 3-[18F]fluoro-4-aminopyridine via Yamada-Curtius rearrangement"

Article Title: An efficient new method for the synthesis of 3-[18F]fluoro-4-aminopyridine via Yamada-Curtius rearrangement

Journal: Journal of labelled compounds & radiopharmaceuticals

doi: 10.1002/jlcr.3560

HPLC analysis of crude A, methyl 3-[ 18 F] fluoroisonicotinate, B, methyl 3-[ 18 F]fluoroisonicotinate coinjected with nonradioactive cold standard, and C, [ 18 F]fluoroisonicotinic acid ( 2 ). HPLC conditions: Agilent Eclipse XDB C18 column (5 µm, 4.6 × 150 mm), mobile phase: 1 to 5% B in 8 minutes, 5 to 90% B in 15 minutes. A = water (0.1% TFA), B = acetonitrile, with a flow rate of 1 mL/min. Red line, in-line radiodetection; black line, UV detection at 254 nm
Figure Legend Snippet: HPLC analysis of crude A, methyl 3-[ 18 F] fluoroisonicotinate, B, methyl 3-[ 18 F]fluoroisonicotinate coinjected with nonradioactive cold standard, and C, [ 18 F]fluoroisonicotinic acid ( 2 ). HPLC conditions: Agilent Eclipse XDB C18 column (5 µm, 4.6 × 150 mm), mobile phase: 1 to 5% B in 8 minutes, 5 to 90% B in 15 minutes. A = water (0.1% TFA), B = acetonitrile, with a flow rate of 1 mL/min. Red line, in-line radiodetection; black line, UV detection at 254 nm

Techniques Used: High Performance Liquid Chromatography, Flow Cytometry

HPLC analysis of A, 3-[ 18 F]fluoro-4-aminopyridine ( 3 ) and B, 3-[ 18 F]fluoro-4-aminopyridine ( 3 ) coinjected with the nonradioactive standard. HPLC conditions: Phenomenex Luna C18 column (5 µm, 4.6 × 100 mm), mobile phase: 5% acetonitrile in water (0.1% triethylamine), with a flow rate of 1 mL/min. Red line, in-line radiodetection; black line, UV detection at 254 nm
Figure Legend Snippet: HPLC analysis of A, 3-[ 18 F]fluoro-4-aminopyridine ( 3 ) and B, 3-[ 18 F]fluoro-4-aminopyridine ( 3 ) coinjected with the nonradioactive standard. HPLC conditions: Phenomenex Luna C18 column (5 µm, 4.6 × 100 mm), mobile phase: 5% acetonitrile in water (0.1% triethylamine), with a flow rate of 1 mL/min. Red line, in-line radiodetection; black line, UV detection at 254 nm

Techniques Used: High Performance Liquid Chromatography, Flow Cytometry

13) Product Images from "A Cross-chiral RNA Polymerase Ribozyme"

Article Title: A Cross-chiral RNA Polymerase Ribozyme

Journal: Nature

doi: 10.1038/nature13900

HPLC analysis of synthetic L-NTPs. a, Elution of the four L-NTPs. b, Elution of the four authentic D-NTPs. HPLC conditions: C18 column, linear gradient of 0– 10% acetonitrile in 20 mM triethylammonium acetate (pH 7.0), UV detection at 254 nm.
Figure Legend Snippet: HPLC analysis of synthetic L-NTPs. a, Elution of the four L-NTPs. b, Elution of the four authentic D-NTPs. HPLC conditions: C18 column, linear gradient of 0– 10% acetonitrile in 20 mM triethylammonium acetate (pH 7.0), UV detection at 254 nm.

Techniques Used: High Performance Liquid Chromatography

14) Product Images from "Detection of 7-(2?-Carboxyethyl)guanine but not 7-Carboxymethylguanine in Human Liver DNA"

Article Title: Detection of 7-(2?-Carboxyethyl)guanine but not 7-Carboxymethylguanine in Human Liver DNA

Journal: Chemical research in toxicology

doi: 10.1021/tx100062v

Chromatograms obtained upon LC-ESI-MS/MS-SRM analysis of a hydrolysate of human liver DNA for the methyl esters of 7-CMGua and 7-CEGua. A Zorbax SB C18 column was used for the analysis. Shaded peaks correspond to the methyl esters of [ 15 N 5 ]7-CMGua internal standard (panel B); 7-CEGua (panel C); and [ 15 N 5 ]7-CEGua internal standard (panel D). No peak was observed corresponding to the methyl ester of 7-CMGua (panel A).
Figure Legend Snippet: Chromatograms obtained upon LC-ESI-MS/MS-SRM analysis of a hydrolysate of human liver DNA for the methyl esters of 7-CMGua and 7-CEGua. A Zorbax SB C18 column was used for the analysis. Shaded peaks correspond to the methyl esters of [ 15 N 5 ]7-CMGua internal standard (panel B); 7-CEGua (panel C); and [ 15 N 5 ]7-CEGua internal standard (panel D). No peak was observed corresponding to the methyl ester of 7-CMGua (panel A).

Techniques Used: Mass Spectrometry

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    Phenomenex c18 column luna
    Effect of iPLA 2 γ inhibitor, (R)-BEL, on SWCNT-induced pulmonary inflammation. ( A ) Production of pro- and anti-inflammatory cytokines in BAL of mice. Measurements were performed using Bio-Plex Pro Mouse Cytokine 23-Plex Immunoassay kit, composed of a combination of pro- and anti-inflammatory cytokines. ( B ) Representative images of lung sections from mice on day 1 post exposure to SWCNT or R-BEL alone, SWCNT/R-BEL together and control group (neutrophils indicated by arrows). ( C ) Average neutrophil counts per whole lung sections from 3 mice per group. ( D ) Tissue damage and fibrogenic response in BAL of mice. Pulmonary tissue damage was measured as protein in BAL of mice on day 1 after exposure to SWCNT (40 μ g/mouse; a). Fibrogenic response was assessed on day 7 post exposure to SWCNT (40 μ g/mouse) by the levels of collagen measured in the lung of mice (b). ( E ) Production of pro-inflammatory lipid mediators, LTB 4 (a), and HXB 3 (b) in lung of C57B6J mice. Structural formulas of LTB 4 and HXB 3 (inserts). ( F ) (R)-BEL treatment reversed the increases in PNLP8 expression (green) and cell death (TUNEL(magenta) positive nuclei, arrows) induced by SWCNT exposure in mouse lungs (a), % PNLP8 positive cells relative to total cell number (b), % TUNEL positive cells relative to total cell number (c). ( G ) MS spectrum of CL obtained from control mouse lung (a). Base peak chromatograms of CL molecular species with m / z 1475.992 from lung of control mouse and mouse exposed to SWCNT in the absence and in the presence of (R)-BEL on day 1 after treatment (b). MS 2 spectrum of CL molecular species with m / z 1475.992 containing arachidonic acid (C20:4), <t>CL-C18:1/C18:2/C18:1/C20:4,</t> obtained from control mouse lung (c). All data are mean ± SD ( n = 4–5 mice per group). * P
    C18 Column Luna, supplied by Phenomenex, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c18 column luna/product/Phenomenex
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Phenomenex c18
    Chromatogram of concentrated methanol extract of ( a ) purified beeswax, ( b ) raw beeswax, and ( c ) green propolis. Chromatographic conditions: <t>C18</t> column with a mobile phase composed of (A) water containing 0.4% formic acid, 5% methanol, and 2% isopropanol, and (B) acetonitrile and 2% isopropanol. The flow was 1 mL/min in the concentration gradient, using 20% B for 3 min, 20–25% B for 3–4 min, 25% B for 4–15 min, 25–45% B for 15–20 min, 45% B for 20–40 min, 45–60% B for 40–45 min, 60–80% B for 45–68.83 min, 80–20% B for 68.86–70 min, and 20% B for 70–80 min. An injection volume of 15 µL, oven temperature of 30 °C, and wavelength of 300 nm were used.
    C18, supplied by Phenomenex, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c18/product/Phenomenex
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    c18 - by Bioz Stars, 2022-09
    88/100 stars
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    90
    Phenomenex luna c18 column
    Effects of Guiera senegalensis hydroethanolic extract (GS: 1 µg/L, 4 µg/L and 8 µg/L) on Sco-induced increasing in the activity of the acetylcholinesterase (AChE). Each <t>column</t> represents mean ± S.E.M. of 10 zebrafish. For Tukey’s post hoc analyses: *** p
    Luna C18 Column, supplied by Phenomenex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luna c18 column/product/Phenomenex
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    luna c18 column - by Bioz Stars, 2022-09
    90/100 stars
      Buy from Supplier

    88
    Phenomenex luna c18 analytical column
    Representative mass spectra of trans -4-hydroxytamoxifen quaternary ammonium glucuronide formed by human liver microsomes. The predicted trans -4-hydroxytamoxifen quaternary ammonium glucuronide ( trans -4-OH-TAM- N + -glucuronide) was collected after separation by HPLC and identified by liquid chromatography–mass spectrometry using a Shimadzu LC-MS 2010 EV system. trans -4-OH-TAM- N + -glucuronide was loaded onto a <t>C18</t> Shimadzu reverse-phase column and analyzed at a flow rate of 0.2 ml/min by applying a linear mobile phase gradient from 10% to 80% (v/v) methanol/H 2 O over 30 min. An electrospray voltage of 1.5 kV was applied using a positive mode.
    Luna C18 Analytical Column, supplied by Phenomenex, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luna c18 analytical column/product/Phenomenex
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    luna c18 analytical column - by Bioz Stars, 2022-09
    88/100 stars
      Buy from Supplier

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    Effect of iPLA 2 γ inhibitor, (R)-BEL, on SWCNT-induced pulmonary inflammation. ( A ) Production of pro- and anti-inflammatory cytokines in BAL of mice. Measurements were performed using Bio-Plex Pro Mouse Cytokine 23-Plex Immunoassay kit, composed of a combination of pro- and anti-inflammatory cytokines. ( B ) Representative images of lung sections from mice on day 1 post exposure to SWCNT or R-BEL alone, SWCNT/R-BEL together and control group (neutrophils indicated by arrows). ( C ) Average neutrophil counts per whole lung sections from 3 mice per group. ( D ) Tissue damage and fibrogenic response in BAL of mice. Pulmonary tissue damage was measured as protein in BAL of mice on day 1 after exposure to SWCNT (40 μ g/mouse; a). Fibrogenic response was assessed on day 7 post exposure to SWCNT (40 μ g/mouse) by the levels of collagen measured in the lung of mice (b). ( E ) Production of pro-inflammatory lipid mediators, LTB 4 (a), and HXB 3 (b) in lung of C57B6J mice. Structural formulas of LTB 4 and HXB 3 (inserts). ( F ) (R)-BEL treatment reversed the increases in PNLP8 expression (green) and cell death (TUNEL(magenta) positive nuclei, arrows) induced by SWCNT exposure in mouse lungs (a), % PNLP8 positive cells relative to total cell number (b), % TUNEL positive cells relative to total cell number (c). ( G ) MS spectrum of CL obtained from control mouse lung (a). Base peak chromatograms of CL molecular species with m / z 1475.992 from lung of control mouse and mouse exposed to SWCNT in the absence and in the presence of (R)-BEL on day 1 after treatment (b). MS 2 spectrum of CL molecular species with m / z 1475.992 containing arachidonic acid (C20:4), CL-C18:1/C18:2/C18:1/C20:4, obtained from control mouse lung (c). All data are mean ± SD ( n = 4–5 mice per group). * P

    Journal: Journal of leukocyte biology

    Article Title: Redox (phospho)lipidomics of signaling in inflammation and programmed cell death

    doi: 10.1002/JLB.3MIR0119-004RR

    Figure Lengend Snippet: Effect of iPLA 2 γ inhibitor, (R)-BEL, on SWCNT-induced pulmonary inflammation. ( A ) Production of pro- and anti-inflammatory cytokines in BAL of mice. Measurements were performed using Bio-Plex Pro Mouse Cytokine 23-Plex Immunoassay kit, composed of a combination of pro- and anti-inflammatory cytokines. ( B ) Representative images of lung sections from mice on day 1 post exposure to SWCNT or R-BEL alone, SWCNT/R-BEL together and control group (neutrophils indicated by arrows). ( C ) Average neutrophil counts per whole lung sections from 3 mice per group. ( D ) Tissue damage and fibrogenic response in BAL of mice. Pulmonary tissue damage was measured as protein in BAL of mice on day 1 after exposure to SWCNT (40 μ g/mouse; a). Fibrogenic response was assessed on day 7 post exposure to SWCNT (40 μ g/mouse) by the levels of collagen measured in the lung of mice (b). ( E ) Production of pro-inflammatory lipid mediators, LTB 4 (a), and HXB 3 (b) in lung of C57B6J mice. Structural formulas of LTB 4 and HXB 3 (inserts). ( F ) (R)-BEL treatment reversed the increases in PNLP8 expression (green) and cell death (TUNEL(magenta) positive nuclei, arrows) induced by SWCNT exposure in mouse lungs (a), % PNLP8 positive cells relative to total cell number (b), % TUNEL positive cells relative to total cell number (c). ( G ) MS spectrum of CL obtained from control mouse lung (a). Base peak chromatograms of CL molecular species with m / z 1475.992 from lung of control mouse and mouse exposed to SWCNT in the absence and in the presence of (R)-BEL on day 1 after treatment (b). MS 2 spectrum of CL molecular species with m / z 1475.992 containing arachidonic acid (C20:4), CL-C18:1/C18:2/C18:1/C20:4, obtained from control mouse lung (c). All data are mean ± SD ( n = 4–5 mice per group). * P

    Article Snippet: Cardiolipins were separated on a C18 column Luna (2) 3 μ m, 100 Å, 150 × 1 mm (Phenomenex) at a flow rate of 0.050 mL/min on a Dionex Ultimate 3000 HPLC system.

    Techniques: Mouse Assay, Expressing, TUNEL Assay, Mass Spectrometry

    Chromatogram of concentrated methanol extract of ( a ) purified beeswax, ( b ) raw beeswax, and ( c ) green propolis. Chromatographic conditions: C18 column with a mobile phase composed of (A) water containing 0.4% formic acid, 5% methanol, and 2% isopropanol, and (B) acetonitrile and 2% isopropanol. The flow was 1 mL/min in the concentration gradient, using 20% B for 3 min, 20–25% B for 3–4 min, 25% B for 4–15 min, 25–45% B for 15–20 min, 45% B for 20–40 min, 45–60% B for 40–45 min, 60–80% B for 45–68.83 min, 80–20% B for 68.86–70 min, and 20% B for 70–80 min. An injection volume of 15 µL, oven temperature of 30 °C, and wavelength of 300 nm were used.

    Journal: Pharmaceutics

    Article Title: A New Approach to Atopic Dermatitis Control with Low-Concentration Propolis-Loaded Cold Cream

    doi: 10.3390/pharmaceutics13091346

    Figure Lengend Snippet: Chromatogram of concentrated methanol extract of ( a ) purified beeswax, ( b ) raw beeswax, and ( c ) green propolis. Chromatographic conditions: C18 column with a mobile phase composed of (A) water containing 0.4% formic acid, 5% methanol, and 2% isopropanol, and (B) acetonitrile and 2% isopropanol. The flow was 1 mL/min in the concentration gradient, using 20% B for 3 min, 20–25% B for 3–4 min, 25% B for 4–15 min, 25–45% B for 15–20 min, 45% B for 20–40 min, 45–60% B for 40–45 min, 60–80% B for 45–68.83 min, 80–20% B for 68.86–70 min, and 20% B for 70–80 min. An injection volume of 15 µL, oven temperature of 30 °C, and wavelength of 300 nm were used.

    Article Snippet: The column used was a C18 (Phenomenex LUNA, Torrance, CA, USA) of 250 mm × 4.6 × 5 μm with a precolumn C18 of 4 mm × 3.0 mm.

    Techniques: Purification, Concentration Assay, Injection

    Effects of Guiera senegalensis hydroethanolic extract (GS: 1 µg/L, 4 µg/L and 8 µg/L) on Sco-induced increasing in the activity of the acetylcholinesterase (AChE). Each column represents mean ± S.E.M. of 10 zebrafish. For Tukey’s post hoc analyses: *** p

    Journal: Plants

    Article Title: Neuroprotective Potential of Guiera senegalensis (Combretaceae) Leaf Hydroethanolic Extract against Cholinergic System Dysfunctions and Oxidative Stress in Scopolamine-Induced Cognitive Impairment in Zebrafish (Danio rerio)

    doi: 10.3390/plants11091149

    Figure Lengend Snippet: Effects of Guiera senegalensis hydroethanolic extract (GS: 1 µg/L, 4 µg/L and 8 µg/L) on Sco-induced increasing in the activity of the acetylcholinesterase (AChE). Each column represents mean ± S.E.M. of 10 zebrafish. For Tukey’s post hoc analyses: *** p

    Article Snippet: Volumes of 5 µL were injected in the LC-PDA Thermo UltiMate 3000 (Pro Analysis Systems, Bucharest, Romania) system equipped with a Luna C18 column (Phenomenex, CA, USA) (150 × 4.6 mm, 100 Å).

    Techniques: Activity Assay

    Representative mass spectra of trans -4-hydroxytamoxifen quaternary ammonium glucuronide formed by human liver microsomes. The predicted trans -4-hydroxytamoxifen quaternary ammonium glucuronide ( trans -4-OH-TAM- N + -glucuronide) was collected after separation by HPLC and identified by liquid chromatography–mass spectrometry using a Shimadzu LC-MS 2010 EV system. trans -4-OH-TAM- N + -glucuronide was loaded onto a C18 Shimadzu reverse-phase column and analyzed at a flow rate of 0.2 ml/min by applying a linear mobile phase gradient from 10% to 80% (v/v) methanol/H 2 O over 30 min. An electrospray voltage of 1.5 kV was applied using a positive mode.

    Journal: Breast Cancer Research

    Article Title: Characterization of tamoxifen and 4-hydroxytamoxifen glucuronidation by human UGT1A4 variants

    doi: 10.1186/bcr1539

    Figure Lengend Snippet: Representative mass spectra of trans -4-hydroxytamoxifen quaternary ammonium glucuronide formed by human liver microsomes. The predicted trans -4-hydroxytamoxifen quaternary ammonium glucuronide ( trans -4-OH-TAM- N + -glucuronide) was collected after separation by HPLC and identified by liquid chromatography–mass spectrometry using a Shimadzu LC-MS 2010 EV system. trans -4-OH-TAM- N + -glucuronide was loaded onto a C18 Shimadzu reverse-phase column and analyzed at a flow rate of 0.2 ml/min by applying a linear mobile phase gradient from 10% to 80% (v/v) methanol/H 2 O over 30 min. An electrospray voltage of 1.5 kV was applied using a positive mode.

    Article Snippet: HPLC was performed using a 3 μ Luna C18 analytical column (4.6 mm × 150 mm; Phenomenex, Torrance, CA, USA) in series with a 5 μ Aquasil C18 guard column (10 mm × 4 mm; Thermo Hypersil-Keystone, Bellefonte, PA, USA).

    Techniques: High Performance Liquid Chromatography, Liquid Chromatography, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy, Flow Cytometry