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Genechem luciferase open reading frame
Luciferase Open Reading Frame, supplied by Genechem, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/luciferase open reading frame/product/Genechem
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
luciferase open reading frame - by Bioz Stars, 2020-09
91/100 stars

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Luciferase:

Article Title: Combination epidermal growth factor receptor variant III peptide-pulsed dendritic cell vaccine with miR-326 results in enhanced killing on EGFRvIII-positive cells
Article Snippet: .. Luciferase reporter assay Wild-type luciferase reporter plasmids GV272-TGF-β1-3′-UTR and GV272-SMO-3′-UTR were created that contained putative miR-326 binding sites for TGF-β1 and the SMO 3′-UTR respectively, downstream of the luciferase open reading frame (Genechem, China). .. Next, we transiently expressed these constructs in U87-EGFRvIII cells in the presence or absence of miR-326 mimics using Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer's protocol.

Reporter Assay:

Article Title: Combination epidermal growth factor receptor variant III peptide-pulsed dendritic cell vaccine with miR-326 results in enhanced killing on EGFRvIII-positive cells
Article Snippet: .. Luciferase reporter assay Wild-type luciferase reporter plasmids GV272-TGF-β1-3′-UTR and GV272-SMO-3′-UTR were created that contained putative miR-326 binding sites for TGF-β1 and the SMO 3′-UTR respectively, downstream of the luciferase open reading frame (Genechem, China). .. Next, we transiently expressed these constructs in U87-EGFRvIII cells in the presence or absence of miR-326 mimics using Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer's protocol.

Binding Assay:

Article Title: Combination epidermal growth factor receptor variant III peptide-pulsed dendritic cell vaccine with miR-326 results in enhanced killing on EGFRvIII-positive cells
Article Snippet: .. Luciferase reporter assay Wild-type luciferase reporter plasmids GV272-TGF-β1-3′-UTR and GV272-SMO-3′-UTR were created that contained putative miR-326 binding sites for TGF-β1 and the SMO 3′-UTR respectively, downstream of the luciferase open reading frame (Genechem, China). .. Next, we transiently expressed these constructs in U87-EGFRvIII cells in the presence or absence of miR-326 mimics using Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer's protocol.

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    Genechem pparα pparα
    Active <t>PPARα</t> feedback represses E2F1 activation ( A ) PPARα activation increases p16 and p21 protein levels and decreases CDKI-mediated pRB phosphorylation. ( B , C ) The PPARα agonist fenofibrate promoted E2F1/RB complex formation in glioma cells.
    Pparα Pparα, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pparα pparα/product/Genechem
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pparα pparα - by Bioz Stars, 2020-09
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      Buy from Supplier

    86
    Genechem pdgfr α β
    miR-34a can directly target <t>PDGFR-α/β</t> (A) The wild-type and mutated sequences of the two predicted miR-34a binding sites within the PDGFR-α3′ UTR and one predicted miR-34a binding site within the PDGFR-β3′ UTR are shown. (B) Co-transfection of miR-34a and the wild-type PDGFR-α/β 3′ UTR caused a significant decrease in luciferase activity compared with the controls. (C) Co-transfection of miR-34a and the mutant PDGFR-α/β3′ UTR plasmids (including all mutants) did not cause a decrease in luciferase activity. The data represent the mean±S.D.;* P
    Pdgfr α β, supplied by Genechem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pdgfr α β/product/Genechem
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    pdgfr α β - by Bioz Stars, 2020-09
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    91
    Genechem luciferase open reading frame
    miR-34a can directly target <t>PDGFR-α/β</t> (A) The wild-type and mutated sequences of the two predicted miR-34a binding sites within the PDGFR-α3′ UTR and one predicted miR-34a binding site within the PDGFR-β3′ UTR are shown. (B) Co-transfection of miR-34a and the wild-type PDGFR-α/β 3′ UTR caused a significant decrease in luciferase activity compared with the controls. (C) Co-transfection of miR-34a and the mutant PDGFR-α/β3′ UTR plasmids (including all mutants) did not cause a decrease in luciferase activity. The data represent the mean±S.D.;* P
    Luciferase Open Reading Frame, supplied by Genechem, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luciferase open reading frame/product/Genechem
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    luciferase open reading frame - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

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    Active PPARα feedback represses E2F1 activation ( A ) PPARα activation increases p16 and p21 protein levels and decreases CDKI-mediated pRB phosphorylation. ( B , C ) The PPARα agonist fenofibrate promoted E2F1/RB complex formation in glioma cells.

    Journal: Oncotarget

    Article Title: PPARα, a predictor of patient survival in glioma, inhibits cell growth through the E2F1/miR-19a feedback loop

    doi: 10.18632/oncotarget.13170

    Figure Lengend Snippet: Active PPARα feedback represses E2F1 activation ( A ) PPARα activation increases p16 and p21 protein levels and decreases CDKI-mediated pRB phosphorylation. ( B , C ) The PPARα agonist fenofibrate promoted E2F1/RB complex formation in glioma cells.

    Article Snippet: The lentiviral vector Ubi-MCS-3FLAG-SV40-EGFP, containing the ORF of PPARα (PPARα), was generated by GeneChem Inc.

    Techniques: Activation Assay

    Effects of PPARα on glioma cell biology as evaluated with in vitro assays ( A ) Overexpression of PPARα decreased the number of colonies formed in plates. ( B ) Invasiveness of U87 and LN229 cells was attenuated by increased expression of PPARα. ( C ) Over-expression of PPARα reduced the levels of aerobic glycolysis in U87 and LN229 cells. ( D ) Representative images of mice implanted with intracranial tumors on days 10, 20, and 30. ( E ) Plot of Fluc activity by bioluminescence imaging for intracranial tumors. ( F ) Kaplan-Meier survival curves for PPARα expression in the two groups of mice. ( G ) The PPARα agonist fenofibrate interferes with the Akt and Erk1/2 signaling pathways by decreasing Akt and Erk1/2 phosphorylation. The effect of fenofibrate on Akt and Erk1/2 phosphorylation in a PPARα-dependent manner.

    Journal: Oncotarget

    Article Title: PPARα, a predictor of patient survival in glioma, inhibits cell growth through the E2F1/miR-19a feedback loop

    doi: 10.18632/oncotarget.13170

    Figure Lengend Snippet: Effects of PPARα on glioma cell biology as evaluated with in vitro assays ( A ) Overexpression of PPARα decreased the number of colonies formed in plates. ( B ) Invasiveness of U87 and LN229 cells was attenuated by increased expression of PPARα. ( C ) Over-expression of PPARα reduced the levels of aerobic glycolysis in U87 and LN229 cells. ( D ) Representative images of mice implanted with intracranial tumors on days 10, 20, and 30. ( E ) Plot of Fluc activity by bioluminescence imaging for intracranial tumors. ( F ) Kaplan-Meier survival curves for PPARα expression in the two groups of mice. ( G ) The PPARα agonist fenofibrate interferes with the Akt and Erk1/2 signaling pathways by decreasing Akt and Erk1/2 phosphorylation. The effect of fenofibrate on Akt and Erk1/2 phosphorylation in a PPARα-dependent manner.

    Article Snippet: The lentiviral vector Ubi-MCS-3FLAG-SV40-EGFP, containing the ORF of PPARα (PPARα), was generated by GeneChem Inc.

    Techniques: In Vitro, Over Expression, Expressing, Mouse Assay, Activity Assay, Imaging

    PPARα is negatively regulated by E2F1/miR-19a signaling in glioma cells ( A ) Schematic of the PPARα 3′UTR including the putative binding sites for miR-19a, as predicted by TargetScan and Pictar algorithms. ( B ) PPARα protein levels in U87 and LN229 cells at 48 h post-transfection. ( C ) MiR-19a down-regulated luciferase activity of wild-type PPARα 3′UTR expression vector, but did not reduce expression of a mutant 3′UTR. ( D ) Heatmap showing the miR-17-92 cluster (including miR-19a) positively correlated with E2F1 in 158 glioma samples. ( E ) si-E2F1 decreases the expression of miR-19a in U87 and LN229 cells. ( F ) si-E2F1 inhibits miPPR-19a activity in a luciferase assay. ( G ) Increased E2F1 expression enhances miPPR-19a activity.

    Journal: Oncotarget

    Article Title: PPARα, a predictor of patient survival in glioma, inhibits cell growth through the E2F1/miR-19a feedback loop

    doi: 10.18632/oncotarget.13170

    Figure Lengend Snippet: PPARα is negatively regulated by E2F1/miR-19a signaling in glioma cells ( A ) Schematic of the PPARα 3′UTR including the putative binding sites for miR-19a, as predicted by TargetScan and Pictar algorithms. ( B ) PPARα protein levels in U87 and LN229 cells at 48 h post-transfection. ( C ) MiR-19a down-regulated luciferase activity of wild-type PPARα 3′UTR expression vector, but did not reduce expression of a mutant 3′UTR. ( D ) Heatmap showing the miR-17-92 cluster (including miR-19a) positively correlated with E2F1 in 158 glioma samples. ( E ) si-E2F1 decreases the expression of miR-19a in U87 and LN229 cells. ( F ) si-E2F1 inhibits miPPR-19a activity in a luciferase assay. ( G ) Increased E2F1 expression enhances miPPR-19a activity.

    Article Snippet: The lentiviral vector Ubi-MCS-3FLAG-SV40-EGFP, containing the ORF of PPARα (PPARα), was generated by GeneChem Inc.

    Techniques: Binding Assay, Transfection, Luciferase, Activity Assay, Expressing, Plasmid Preparation, Mutagenesis

    E2F1 affects the biological behavior of glioma cells, modulating PPARα in a miR-19a-dependent manner ( A ) The levels of E2F1 were analyzed in glioma tissues of the CGGA glioma datasets (61cases of LGG, 97 cases of HGG). ( B ) qRT-PCR confirmation of decreased E2F1 levels in HGG tissues compared with LGG tissues and normal brain tissues. ( C ) Kaplan-Meier survival curves for E2F1 expression in glioma tissues of the CGGA dataset. High expression of E2F1 confers a poor prognosis in glioma patients. ( D ) Decreased E2F1 suppressed the proliferation of glioma cells and its effects were blockedby miR-19a. ( E ) Decreased E2F1 suppressed the proliferation of glioma cells and its effects were blockedby miR-19a. ( F ) The invasiveness of U87 and LN229 cells was attenuated with the increased expression of E2F1. The effect of E2F1 on glioma cells is abolished by miR-19a. ( G ) Western blots identified that miR-19a could restore siE2F1 inducing PPARα expression decreased.

    Journal: Oncotarget

    Article Title: PPARα, a predictor of patient survival in glioma, inhibits cell growth through the E2F1/miR-19a feedback loop

    doi: 10.18632/oncotarget.13170

    Figure Lengend Snippet: E2F1 affects the biological behavior of glioma cells, modulating PPARα in a miR-19a-dependent manner ( A ) The levels of E2F1 were analyzed in glioma tissues of the CGGA glioma datasets (61cases of LGG, 97 cases of HGG). ( B ) qRT-PCR confirmation of decreased E2F1 levels in HGG tissues compared with LGG tissues and normal brain tissues. ( C ) Kaplan-Meier survival curves for E2F1 expression in glioma tissues of the CGGA dataset. High expression of E2F1 confers a poor prognosis in glioma patients. ( D ) Decreased E2F1 suppressed the proliferation of glioma cells and its effects were blockedby miR-19a. ( E ) Decreased E2F1 suppressed the proliferation of glioma cells and its effects were blockedby miR-19a. ( F ) The invasiveness of U87 and LN229 cells was attenuated with the increased expression of E2F1. The effect of E2F1 on glioma cells is abolished by miR-19a. ( G ) Western blots identified that miR-19a could restore siE2F1 inducing PPARα expression decreased.

    Article Snippet: The lentiviral vector Ubi-MCS-3FLAG-SV40-EGFP, containing the ORF of PPARα (PPARα), was generated by GeneChem Inc.

    Techniques: Quantitative RT-PCR, Expressing, Western Blot

    E2F1/ miR-19a/ PPARα signaling was confirmed in human glioma tissues and in nude mice orthotopic glioma model ( A ) Expression of E2F1, miR-19a and PPARα in human glioma tissues by IHC and ISH. ( B ) E2F1/ miR-19a/ PPARα feedback loop was confirmed in nude mouse orthotopic glioma model.

    Journal: Oncotarget

    Article Title: PPARα, a predictor of patient survival in glioma, inhibits cell growth through the E2F1/miR-19a feedback loop

    doi: 10.18632/oncotarget.13170

    Figure Lengend Snippet: E2F1/ miR-19a/ PPARα signaling was confirmed in human glioma tissues and in nude mice orthotopic glioma model ( A ) Expression of E2F1, miR-19a and PPARα in human glioma tissues by IHC and ISH. ( B ) E2F1/ miR-19a/ PPARα feedback loop was confirmed in nude mouse orthotopic glioma model.

    Article Snippet: The lentiviral vector Ubi-MCS-3FLAG-SV40-EGFP, containing the ORF of PPARα (PPARα), was generated by GeneChem Inc.

    Techniques: Mouse Assay, Expressing, Immunohistochemistry, In Situ Hybridization

    PPARα expression in glioma tissues of the CGGA glioma dataset (61 cases of low grade glioma [LGG], 97 cases of high grade glioma [HGG]) ( A ) PPARα expression was significantly lower in HGGtissues than in LGG tissues. ( B ) qRT-PCR confirmation of reduced PPARα levels in HGG tissues compared with LGG tissues and normal brain tissues. ( C ) Kaplan-Meier survival curves for PPARα expression in glioma tissues of the CGGA dataset. Low expression of PPARα confers a poor prognosis in glioma patients.

    Journal: Oncotarget

    Article Title: PPARα, a predictor of patient survival in glioma, inhibits cell growth through the E2F1/miR-19a feedback loop

    doi: 10.18632/oncotarget.13170

    Figure Lengend Snippet: PPARα expression in glioma tissues of the CGGA glioma dataset (61 cases of low grade glioma [LGG], 97 cases of high grade glioma [HGG]) ( A ) PPARα expression was significantly lower in HGGtissues than in LGG tissues. ( B ) qRT-PCR confirmation of reduced PPARα levels in HGG tissues compared with LGG tissues and normal brain tissues. ( C ) Kaplan-Meier survival curves for PPARα expression in glioma tissues of the CGGA dataset. Low expression of PPARα confers a poor prognosis in glioma patients.

    Article Snippet: The lentiviral vector Ubi-MCS-3FLAG-SV40-EGFP, containing the ORF of PPARα (PPARα), was generated by GeneChem Inc.

    Techniques: Expressing, Quantitative RT-PCR

    miR-34a can directly target PDGFR-α/β (A) The wild-type and mutated sequences of the two predicted miR-34a binding sites within the PDGFR-α3′ UTR and one predicted miR-34a binding site within the PDGFR-β3′ UTR are shown. (B) Co-transfection of miR-34a and the wild-type PDGFR-α/β 3′ UTR caused a significant decrease in luciferase activity compared with the controls. (C) Co-transfection of miR-34a and the mutant PDGFR-α/β3′ UTR plasmids (including all mutants) did not cause a decrease in luciferase activity. The data represent the mean±S.D.;* P

    Journal: Bioscience Reports

    Article Title: MicroRNA-34A inhibits the growth, invasion and metastasis of gastric cancer by targeting PDGFR and MET expression

    doi: 10.1042/BSR20140020

    Figure Lengend Snippet: miR-34a can directly target PDGFR-α/β (A) The wild-type and mutated sequences of the two predicted miR-34a binding sites within the PDGFR-α3′ UTR and one predicted miR-34a binding site within the PDGFR-β3′ UTR are shown. (B) Co-transfection of miR-34a and the wild-type PDGFR-α/β 3′ UTR caused a significant decrease in luciferase activity compared with the controls. (C) Co-transfection of miR-34a and the mutant PDGFR-α/β3′ UTR plasmids (including all mutants) did not cause a decrease in luciferase activity. The data represent the mean±S.D.;* P

    Article Snippet: pcDNA expression plasmids and plasmid transfection The ORF sequences of PDGFR-α/β and MET were amplified from genomic DNA isolated from the AGS cell line and were then subcloned into the GV230 vector (GeneChem Corporation, Shanghai, China).

    Techniques: Binding Assay, Cotransfection, Luciferase, Activity Assay, Mutagenesis

    miR-34a inhibits gastric cancer cell migration, invasion and proliferation, in part, by targeting PDGFR (A) Transient transfection of miR-34a mimics significantly increased miR-34a expression in the AGS cell line. (B) The proliferation of AGS cells was significantly reduced after miR-34a transfection compared with cont-miR transfection.(C) miR-34a significantly reduced the migration and invasion of the AGS cells compared with the controls. (D) Immunoblot analysis of PDGFR expression in AGS cells transfected with miR-34a mimics or cont-miR with or without PDGFR restoration. PDGFR-α/β expression was significantly decreased after transfection with miR-34a mimics, but PDGFR-α/β expression was restored by the overexpression of PDGFR-α/β. (E,F) Gastric cancer cell migration, invasion and proliferation were partially restored after PDGFR restoration. The data represent the mean±S.D.; * P

    Journal: Bioscience Reports

    Article Title: MicroRNA-34A inhibits the growth, invasion and metastasis of gastric cancer by targeting PDGFR and MET expression

    doi: 10.1042/BSR20140020

    Figure Lengend Snippet: miR-34a inhibits gastric cancer cell migration, invasion and proliferation, in part, by targeting PDGFR (A) Transient transfection of miR-34a mimics significantly increased miR-34a expression in the AGS cell line. (B) The proliferation of AGS cells was significantly reduced after miR-34a transfection compared with cont-miR transfection.(C) miR-34a significantly reduced the migration and invasion of the AGS cells compared with the controls. (D) Immunoblot analysis of PDGFR expression in AGS cells transfected with miR-34a mimics or cont-miR with or without PDGFR restoration. PDGFR-α/β expression was significantly decreased after transfection with miR-34a mimics, but PDGFR-α/β expression was restored by the overexpression of PDGFR-α/β. (E,F) Gastric cancer cell migration, invasion and proliferation were partially restored after PDGFR restoration. The data represent the mean±S.D.; * P

    Article Snippet: pcDNA expression plasmids and plasmid transfection The ORF sequences of PDGFR-α/β and MET were amplified from genomic DNA isolated from the AGS cell line and were then subcloned into the GV230 vector (GeneChem Corporation, Shanghai, China).

    Techniques: Migration, Transfection, Expressing, Over Expression

    miRNA selection using qRT-PCR and Western blot analyses (A) The expression levels of 16 miRNAs in gastric cancer cell lines. The expression of these 16 miRNAs was detected using qRT–PCR analysis, and the expression levels of the mature miRNAs were normalized to the levels of the U6 small nuclear RNA. (B) The expression of PDGFR-α/β in gastric cancer cell lines. The expression of PDGFR-α/β was examined using Western blot analysis after the forced expression of miR-29a/b/c, miR-449a/b and miR-34a/c. The data represent the mean±S.D.;* P

    Journal: Bioscience Reports

    Article Title: MicroRNA-34A inhibits the growth, invasion and metastasis of gastric cancer by targeting PDGFR and MET expression

    doi: 10.1042/BSR20140020

    Figure Lengend Snippet: miRNA selection using qRT-PCR and Western blot analyses (A) The expression levels of 16 miRNAs in gastric cancer cell lines. The expression of these 16 miRNAs was detected using qRT–PCR analysis, and the expression levels of the mature miRNAs were normalized to the levels of the U6 small nuclear RNA. (B) The expression of PDGFR-α/β in gastric cancer cell lines. The expression of PDGFR-α/β was examined using Western blot analysis after the forced expression of miR-29a/b/c, miR-449a/b and miR-34a/c. The data represent the mean±S.D.;* P

    Article Snippet: pcDNA expression plasmids and plasmid transfection The ORF sequences of PDGFR-α/β and MET were amplified from genomic DNA isolated from the AGS cell line and were then subcloned into the GV230 vector (GeneChem Corporation, Shanghai, China).

    Techniques: Selection, Quantitative RT-PCR, Western Blot, Expressing