luc activity  (Millipore)


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    Name:
    Luciferase
    Description:
    Luciferase is a class of enzymes naturally found in organisms like protists fungi insects bacteria which belong to phylogenic kingdom It is used as a molecular marker for biological imaging as it specifically cleaves luciferin substrate which results in emitting light
    Catalog Number:
    10411523001
    Price:
    None
    Applications:
    Determination of ATP in ATP-generating or ATP-consuming reactions. Determination of the activity of enzymes such as creatine kinase and ATPases. Under optimal conditions, 1 photon is released per molecule ATP in the bioluminescence assay.One mg is sufficient for approximately 5,000 ATP determinations in the concentration range of 0.1 nM to 1 muM.
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    Structured Review

    Millipore luc activity
    Regulation of 4E-BP1 promoter activity by co-Smads. Firefly <t>LUC</t> activity normalized as in was assayed in different cell lines <t>co-transfected</t> with the −628/+219 4E-BP1 promoter fragment and various combinations of Smad2, 3, 4
    Luciferase is a class of enzymes naturally found in organisms like protists fungi insects bacteria which belong to phylogenic kingdom It is used as a molecular marker for biological imaging as it specifically cleaves luciferin substrate which results in emitting light
    https://www.bioz.com/result/luc activity/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    luc activity - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "4E-BP1 is a target of Smad4 essential for TGF?-mediated inhibition of cell proliferation"

    Article Title: 4E-BP1 is a target of Smad4 essential for TGF?-mediated inhibition of cell proliferation

    Journal: The EMBO Journal

    doi: 10.1038/emboj.2009.291

    Regulation of 4E-BP1 promoter activity by co-Smads. Firefly LUC activity normalized as in was assayed in different cell lines co-transfected with the −628/+219 4E-BP1 promoter fragment and various combinations of Smad2, 3, 4
    Figure Legend Snippet: Regulation of 4E-BP1 promoter activity by co-Smads. Firefly LUC activity normalized as in was assayed in different cell lines co-transfected with the −628/+219 4E-BP1 promoter fragment and various combinations of Smad2, 3, 4

    Techniques Used: Activity Assay, Transfection

    Related Articles

    Clone Assay:

    Article Title: Cloning and characterization of rabbit Rgs4 promoter in gut smooth muscle
    Article Snippet: .. This fragment (designed as Rgs4-P1) was inserted by T-A cloning into the lineated promoter-less pMlu3 AccepTor vector at EcoRV of multiple cloning sties upstream of the secreted renilla luciferase reporter (EMD-Bioscience/Novagen). .. The direction and sequence of the insert were validated by sequencing with upstream and downstream vector primers ( ).

    Transfection:

    Article Title: Viral Source-Independent High Susceptibility of Dendritic Cells to Human T-Cell Leukemia Virus Type 1 Infection Compared to That of T Lymphocytes
    Article Snippet: .. Jurkat-LTR-Luc cells, stably transfected with a plasmid encoding luciferase under the control of the HTLV-1 long terminal repeat (LTR) promoter, were maintained under hygromycin (450 μg/ml, Sigma) selection. .. Jurkat-LTR-GFP cells were maintained under G418 (750 μg/ml; Gibco Life Technologies) selection.

    Article Title: NF-kappaB Mediated Transcriptional Repression of Acid Modifying Hormone Gastrin
    Article Snippet: .. AGS cells transfected with 240 Gas-Luc were treated with Trichostatin A (Calbiochem-Novabiochem, SanDiego, CA), a histone deactylase inhibitor, at 100 nM for 48 hr and harvested for further experiments. .. The siRNA directed against TAK1 (Santa Cruz Biotechnology, CA), p300 (Ambion, Life Technologies, Foster City, USA) and control scrambled siRNA (Ambion, Life Technologies, Foster City, USA) were used at a final concentration of 80 nM for 48 hr.

    Article Title: miR-376a inhibits breast cancer cell progression by targeting neuropilin-1 NR
    Article Snippet: .. Briefly, MCF-7 and MDA-MB-231 cells transfected with Luc-NRP-1-wt or Luc-NRP-1-mut were lysed with 25 mM Tris-HCl buffer (pH 7.5) and 100 U/mL RNase inhibitor (Sigma-Aldrich Co.), and then incubated with protein-A sepharose beads pre-coated with 3 µg anti-Ago2 antibody or control rabbit IgG for 1.5 hours at 4°C. .. The RNA–protein complexes were pulled down by protein A/G agarose beads, and RNA was extracted with TransZol Up, and miR-376a level was measured by qRT-PCR.

    Luciferase:

    Article Title: FEZF1-AS1 is a key regulator of cell cycle, epithelial–mesenchymal transition and Wnt/β-catenin signaling in nasopharyngeal carcinoma cells
    Article Snippet: .. Dual luciferase reporter assay Cells were plated in 24-well plates and cotransfected with pcDNA3.1 vector, TOP Flash or FOP Flash reporter (Millipore, Temecula, CA, U.S.A.) and pRL-TK plasmids (Millipore) using Lipofectamine 2000. .. The luciferase signals were measured 48 h after transfection by using the Dual Luciferase Reporter Assay Kit (Promega).

    Article Title: Viral Source-Independent High Susceptibility of Dendritic Cells to Human T-Cell Leukemia Virus Type 1 Infection Compared to That of T Lymphocytes
    Article Snippet: .. Jurkat-LTR-Luc cells, stably transfected with a plasmid encoding luciferase under the control of the HTLV-1 long terminal repeat (LTR) promoter, were maintained under hygromycin (450 μg/ml, Sigma) selection. .. Jurkat-LTR-GFP cells were maintained under G418 (750 μg/ml; Gibco Life Technologies) selection.

    Article Title: dREAM co-operates with insulator-binding proteins and regulates expression at divergently paired genes
    Article Snippet: .. Drosophila tissue culture and Luciferase reporter assay S2 cells were maintained in Shields and Sang M3 medium (Sigma) with 10% fetal bovine serum, BPYE and 1% Penicillin/Streptomycin. .. For the enhancer-blocking assay, S2 cells were transfected with 100 ng of the enhancer-blocking reporter and 250 ng of the Renilla control plasmid, using the X-tremeGENE HP transfection reagent (Roche).

    Article Title: Cloning and characterization of rabbit Rgs4 promoter in gut smooth muscle
    Article Snippet: .. This fragment (designed as Rgs4-P1) was inserted by T-A cloning into the lineated promoter-less pMlu3 AccepTor vector at EcoRV of multiple cloning sties upstream of the secreted renilla luciferase reporter (EMD-Bioscience/Novagen). .. The direction and sequence of the insert were validated by sequencing with upstream and downstream vector primers ( ).

    Reporter Assay:

    Article Title: FEZF1-AS1 is a key regulator of cell cycle, epithelial–mesenchymal transition and Wnt/β-catenin signaling in nasopharyngeal carcinoma cells
    Article Snippet: .. Dual luciferase reporter assay Cells were plated in 24-well plates and cotransfected with pcDNA3.1 vector, TOP Flash or FOP Flash reporter (Millipore, Temecula, CA, U.S.A.) and pRL-TK plasmids (Millipore) using Lipofectamine 2000. .. The luciferase signals were measured 48 h after transfection by using the Dual Luciferase Reporter Assay Kit (Promega).

    Article Title: dREAM co-operates with insulator-binding proteins and regulates expression at divergently paired genes
    Article Snippet: .. Drosophila tissue culture and Luciferase reporter assay S2 cells were maintained in Shields and Sang M3 medium (Sigma) with 10% fetal bovine serum, BPYE and 1% Penicillin/Streptomycin. .. For the enhancer-blocking assay, S2 cells were transfected with 100 ng of the enhancer-blocking reporter and 250 ng of the Renilla control plasmid, using the X-tremeGENE HP transfection reagent (Roche).

    Stable Transfection:

    Article Title: Viral Source-Independent High Susceptibility of Dendritic Cells to Human T-Cell Leukemia Virus Type 1 Infection Compared to That of T Lymphocytes
    Article Snippet: .. Jurkat-LTR-Luc cells, stably transfected with a plasmid encoding luciferase under the control of the HTLV-1 long terminal repeat (LTR) promoter, were maintained under hygromycin (450 μg/ml, Sigma) selection. .. Jurkat-LTR-GFP cells were maintained under G418 (750 μg/ml; Gibco Life Technologies) selection.

    Multiple Displacement Amplification:

    Article Title: miR-376a inhibits breast cancer cell progression by targeting neuropilin-1 NR
    Article Snippet: .. Briefly, MCF-7 and MDA-MB-231 cells transfected with Luc-NRP-1-wt or Luc-NRP-1-mut were lysed with 25 mM Tris-HCl buffer (pH 7.5) and 100 U/mL RNase inhibitor (Sigma-Aldrich Co.), and then incubated with protein-A sepharose beads pre-coated with 3 µg anti-Ago2 antibody or control rabbit IgG for 1.5 hours at 4°C. .. The RNA–protein complexes were pulled down by protein A/G agarose beads, and RNA was extracted with TransZol Up, and miR-376a level was measured by qRT-PCR.

    Blocking Assay:

    Article Title: A novel bioassay for anti-thyrotrophin receptor autoantibodies detects both thyroid-blocking and stimulating activity
    Article Snippet: .. Chimeric CHO-Luc or wild-type CHO-Luc cells were induced with bovine TSH (bTSH) (Sigma Aldrich) mixed with blocking monoclonal antibody (mAb) K1-70, stimulating mAb, M22 (RSR, Cardiff, UK) or human sera. ..

    Incubation:

    Article Title: miR-376a inhibits breast cancer cell progression by targeting neuropilin-1 NR
    Article Snippet: .. Briefly, MCF-7 and MDA-MB-231 cells transfected with Luc-NRP-1-wt or Luc-NRP-1-mut were lysed with 25 mM Tris-HCl buffer (pH 7.5) and 100 U/mL RNase inhibitor (Sigma-Aldrich Co.), and then incubated with protein-A sepharose beads pre-coated with 3 µg anti-Ago2 antibody or control rabbit IgG for 1.5 hours at 4°C. .. The RNA–protein complexes were pulled down by protein A/G agarose beads, and RNA was extracted with TransZol Up, and miR-376a level was measured by qRT-PCR.

    Selection:

    Article Title: Viral Source-Independent High Susceptibility of Dendritic Cells to Human T-Cell Leukemia Virus Type 1 Infection Compared to That of T Lymphocytes
    Article Snippet: .. Jurkat-LTR-Luc cells, stably transfected with a plasmid encoding luciferase under the control of the HTLV-1 long terminal repeat (LTR) promoter, were maintained under hygromycin (450 μg/ml, Sigma) selection. .. Jurkat-LTR-GFP cells were maintained under G418 (750 μg/ml; Gibco Life Technologies) selection.

    Plasmid Preparation:

    Article Title: FEZF1-AS1 is a key regulator of cell cycle, epithelial–mesenchymal transition and Wnt/β-catenin signaling in nasopharyngeal carcinoma cells
    Article Snippet: .. Dual luciferase reporter assay Cells were plated in 24-well plates and cotransfected with pcDNA3.1 vector, TOP Flash or FOP Flash reporter (Millipore, Temecula, CA, U.S.A.) and pRL-TK plasmids (Millipore) using Lipofectamine 2000. .. The luciferase signals were measured 48 h after transfection by using the Dual Luciferase Reporter Assay Kit (Promega).

    Article Title: Viral Source-Independent High Susceptibility of Dendritic Cells to Human T-Cell Leukemia Virus Type 1 Infection Compared to That of T Lymphocytes
    Article Snippet: .. Jurkat-LTR-Luc cells, stably transfected with a plasmid encoding luciferase under the control of the HTLV-1 long terminal repeat (LTR) promoter, were maintained under hygromycin (450 μg/ml, Sigma) selection. .. Jurkat-LTR-GFP cells were maintained under G418 (750 μg/ml; Gibco Life Technologies) selection.

    Article Title: Cloning and characterization of rabbit Rgs4 promoter in gut smooth muscle
    Article Snippet: .. This fragment (designed as Rgs4-P1) was inserted by T-A cloning into the lineated promoter-less pMlu3 AccepTor vector at EcoRV of multiple cloning sties upstream of the secreted renilla luciferase reporter (EMD-Bioscience/Novagen). .. The direction and sequence of the insert were validated by sequencing with upstream and downstream vector primers ( ).

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  • 99
    Millipore renilla luciferase reporter
    Promoter activity of rabbit Rgs4 5′-flanked region (1.4 kb) in various cells. Cultured cells were cotransfected with promoter-less pMlu3 vector or Rgs4 promoter vector carrying secreted <t>renilla</t> luciferase and pGL4-CMV vector carrying firefly luciferase.
    Renilla Luciferase Reporter, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/renilla luciferase reporter/product/Millipore
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    renilla luciferase reporter - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Promoter activity of rabbit Rgs4 5′-flanked region (1.4 kb) in various cells. Cultured cells were cotransfected with promoter-less pMlu3 vector or Rgs4 promoter vector carrying secreted renilla luciferase and pGL4-CMV vector carrying firefly luciferase.

    Journal: Gene

    Article Title: Cloning and characterization of rabbit Rgs4 promoter in gut smooth muscle

    doi: 10.1016/j.gene.2009.11.010

    Figure Lengend Snippet: Promoter activity of rabbit Rgs4 5′-flanked region (1.4 kb) in various cells. Cultured cells were cotransfected with promoter-less pMlu3 vector or Rgs4 promoter vector carrying secreted renilla luciferase and pGL4-CMV vector carrying firefly luciferase.

    Article Snippet: This fragment (designed as Rgs4-P1) was inserted by T-A cloning into the lineated promoter-less pMlu3 AccepTor vector at EcoRV of multiple cloning sties upstream of the secreted renilla luciferase reporter (EMD-Bioscience/Novagen).

    Techniques: Activity Assay, Cell Culture, Plasmid Preparation, Luciferase

    The activity of WNT/β-catenin pathway detected by western blot analysis and TOP/FOP flash experiments. The western blot results showed that overexpression of MEG3 resulted in decreased expression of β-catenin protein compared with the control group, so as to decrease the levels of downstream TCF-4 and cyclin D1 protein, while opposite results were found in cells with MEG3 silencing. The results of TOP/FOP flash experiments showed that the activity of luciferase was decreased by the transfection of MEG3 overexpression vector compared with the control group, while the activity of luciferase was increased after transfecting with MEG3 interference vector (*p

    Journal: Oncology Letters

    Article Title: Long non-coding RNA MEG3 inhibits the proliferation and metastasis of oral squamous cell carcinoma by regulating the WNT/β-catenin signaling pathway

    doi: 10.3892/ol.2017.6682

    Figure Lengend Snippet: The activity of WNT/β-catenin pathway detected by western blot analysis and TOP/FOP flash experiments. The western blot results showed that overexpression of MEG3 resulted in decreased expression of β-catenin protein compared with the control group, so as to decrease the levels of downstream TCF-4 and cyclin D1 protein, while opposite results were found in cells with MEG3 silencing. The results of TOP/FOP flash experiments showed that the activity of luciferase was decreased by the transfection of MEG3 overexpression vector compared with the control group, while the activity of luciferase was increased after transfecting with MEG3 interference vector (*p

    Article Snippet: Cell Counting Kit-8 (CCK-8) (Dojindo, Kumamoto, Japan); Lipofectamine 3000 (Invitrogen Life Technologies, New York, NY, USA); Annexin V-FITC cell apoptosis detection kit (eBioscience, San Diego, CA, USA); 5-aza-2′-deoxycytidine (5-aza-CdR; Sigma, New York, NY, USA); TOP/FOP-Flash luciferase reporter vector (Millipore, Billerica, MA, USA); Dual-Luciferase assay kit (Promega, Madison, WI, USA); primers (Sangon, Shanghai, China).

    Techniques: Activity Assay, Western Blot, Over Expression, Expressing, Luciferase, Transfection, Plasmid Preparation

    The anti-miR-200a effects are partially attenuated by silencing of CTNNB1 in WB-anti-miR-200a cells. (A) The protein levels of β-catenin, c-myc and cyclin D1 measured by western blot analysis after siCTNNB1 transfection in WB-anti-miR-200a cells. (B) The β-catenin-mediated transcription activity determined by the Top/Fop ratio after siCTNNB1 transfection in WB-anti-miR-200a cells. Data are represented as the mean ± SD; n = 3; **, p

    Journal: PLoS ONE

    Article Title: Downregulation of miR-200a Induces EMT Phenotypes and CSC-like Signatures through Targeting the ?-catenin Pathway in Hepatic Oval Cells

    doi: 10.1371/journal.pone.0079409

    Figure Lengend Snippet: The anti-miR-200a effects are partially attenuated by silencing of CTNNB1 in WB-anti-miR-200a cells. (A) The protein levels of β-catenin, c-myc and cyclin D1 measured by western blot analysis after siCTNNB1 transfection in WB-anti-miR-200a cells. (B) The β-catenin-mediated transcription activity determined by the Top/Fop ratio after siCTNNB1 transfection in WB-anti-miR-200a cells. Data are represented as the mean ± SD; n = 3; **, p

    Article Snippet: Briefly, one day before transfection, cells were plated in a 24-well plate and were then transiently transfected with 100 ng of β-catenin-responsive firefly luciferase reporter plasmid TopFlash (Millipore) or negative control FopFlash (Millipore), as well as 10 ng of internal control pRL-TK Renilla luciferase vector (Promega) using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions.

    Techniques: Western Blot, Transfection, Activity Assay

    miR-200a directly targets CTNNB1 and associates with Wnt/β-catenin pathway activity in WB cells. (A) Predicted alignment of miR-200a and a potential binding site at the 3′-UTR of rat CTNNB1 mRNA (624–630 nt) by TargetScan. (B) Effect of miR-200a on CTNNB1 expression determined by a luciferase reporter assay. WB cells were co-transfected with anti-miR-200a (or anti-miR-control) and the pGL3-CTNNB1-wt (or pGL3-CTNNB1-mut) vector. Data are normalized by the ratio of Firefly and Renilla luciferase activities measured at 48 h post-transfection and are shown as the mean ± SD; n = 3; **, p

    Journal: PLoS ONE

    Article Title: Downregulation of miR-200a Induces EMT Phenotypes and CSC-like Signatures through Targeting the ?-catenin Pathway in Hepatic Oval Cells

    doi: 10.1371/journal.pone.0079409

    Figure Lengend Snippet: miR-200a directly targets CTNNB1 and associates with Wnt/β-catenin pathway activity in WB cells. (A) Predicted alignment of miR-200a and a potential binding site at the 3′-UTR of rat CTNNB1 mRNA (624–630 nt) by TargetScan. (B) Effect of miR-200a on CTNNB1 expression determined by a luciferase reporter assay. WB cells were co-transfected with anti-miR-200a (or anti-miR-control) and the pGL3-CTNNB1-wt (or pGL3-CTNNB1-mut) vector. Data are normalized by the ratio of Firefly and Renilla luciferase activities measured at 48 h post-transfection and are shown as the mean ± SD; n = 3; **, p

    Article Snippet: Briefly, one day before transfection, cells were plated in a 24-well plate and were then transiently transfected with 100 ng of β-catenin-responsive firefly luciferase reporter plasmid TopFlash (Millipore) or negative control FopFlash (Millipore), as well as 10 ng of internal control pRL-TK Renilla luciferase vector (Promega) using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions.

    Techniques: Activity Assay, Western Blot, Binding Assay, Expressing, Luciferase, Reporter Assay, Transfection, Plasmid Preparation

    FEZF1-AS1 activates Wnt/β-catenin signaling in NPC cells ( A ) Dual luciferase reporter assay using TOP/FOP flash vectors was performed to determine the activity of Wnt/β-catenin signaling in 5-8F and HNE1 cells after transfection. ( B ) The expression levels of β-catenin in the nuclear and cytosolic fractions of 5-8F and HNE1 cells after transfection were detected by Western blot analysis. Data are presented as the mean ± SD from three independent experiments in vitro . * P

    Journal: Bioscience Reports

    Article Title: FEZF1-AS1 is a key regulator of cell cycle, epithelial–mesenchymal transition and Wnt/β-catenin signaling in nasopharyngeal carcinoma cells

    doi: 10.1042/BSR20180906

    Figure Lengend Snippet: FEZF1-AS1 activates Wnt/β-catenin signaling in NPC cells ( A ) Dual luciferase reporter assay using TOP/FOP flash vectors was performed to determine the activity of Wnt/β-catenin signaling in 5-8F and HNE1 cells after transfection. ( B ) The expression levels of β-catenin in the nuclear and cytosolic fractions of 5-8F and HNE1 cells after transfection were detected by Western blot analysis. Data are presented as the mean ± SD from three independent experiments in vitro . * P

    Article Snippet: Dual luciferase reporter assay Cells were plated in 24-well plates and cotransfected with pcDNA3.1 vector, TOP Flash or FOP Flash reporter (Millipore, Temecula, CA, U.S.A.) and pRL-TK plasmids (Millipore) using Lipofectamine 2000.

    Techniques: Luciferase, Reporter Assay, Activity Assay, Transfection, Expressing, Western Blot, In Vitro