ltq tandem mass spectrometer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ltq tandem mass spectrometer
    Ltq Tandem Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltq tandem mass spectrometer/product/Thermo Fisher
    Average 88 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    ltq tandem mass spectrometer - by Bioz Stars, 2020-05
    88/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Phosphorylation-Specific MS/MS Scoring for Rapid and Accurate Phosphoproteome Analysis
    Article Snippet: .. Mass spectrometry experiments were performed using the 1100 QuadPump HPLC system (Agilent, Santa Clara, CA), the Ultimate 3000 autosampler (Dionex, Sunnyvale, CA), and the LTQ tandem mass spectrometer (Thermo Fischer Scientific, San Jose, CA). .. Four microliters of each eluted fraction was loaded using the autosampler via a 5 μ L sample loop directly to an in-house packed 125 μ m (inner diameter) × 20 cm microcapillary RP-HPLC column, packed with 3 μ m C18 resin (Magic beads; Michrom Bioresources, Auburn, CA).

    Article Title: Proteomic analysis of aqueous humor from patients with myopia
    Article Snippet: .. Liquid chromatography/mass spectrometry/mass spectrometry (LC MS/MS) analysis was performed in an LTQ tandem mass spectrometer (ThermoFinnigan, San Jose, CA) coupled online with an HPLC system. .. Protein digests obtained above were loaded on the HPLC, which was connected with an in-house made fused silica capillary column (10-cm-length; 100 µm inner diameter) and packed with sunchrom 5 μm C18 resin.

    Injection:

    Article Title: LC-MS/MS analysis of differential centrifugation fractions from native inner medullary collecting duct of rat
    Article Snippet: .. Peptides recovered from gel slices were injected into a reverse-phase liquid chromatographic (LC) column (PicoFritTM, Biobasic C18, New Objective, Woodburn, MA) for further separation, and then delivered into an LTQ tandem mass spectrometer (Thermo Electron, San Jose, CA) via electrospray. ..

    Article Title: Large-scale quantitative LC-MS/MS analysis of detergent-resistant membrane proteins from rat renal collecting duct
    Article Snippet: .. Tryptic peptides extracted from each gel slice were injected into a reverse-phase LC column (PicoFrit, Biobasic C18, New Objective, Woodburn, MA) to stratify sample proteins before delivery to an LTQ tandem mass spectrometer (MS/MS, Thermo Electron, San Jose, CA) via a nanoelectrospray ion source. .. The spectra with a total ion current > 10,000 were used to search for matches to peptides in a concatenated RefSeq database using Bioworks software (version 3.1, Thermo Electron) based on the Sequest algorithm.

    Mass Spectrometry:

    Article Title: Phosphorylation-Specific MS/MS Scoring for Rapid and Accurate Phosphoproteome Analysis
    Article Snippet: .. Mass spectrometry experiments were performed using the 1100 QuadPump HPLC system (Agilent, Santa Clara, CA), the Ultimate 3000 autosampler (Dionex, Sunnyvale, CA), and the LTQ tandem mass spectrometer (Thermo Fischer Scientific, San Jose, CA). .. Four microliters of each eluted fraction was loaded using the autosampler via a 5 μ L sample loop directly to an in-house packed 125 μ m (inner diameter) × 20 cm microcapillary RP-HPLC column, packed with 3 μ m C18 resin (Magic beads; Michrom Bioresources, Auburn, CA).

    Article Title: LC-MS/MS analysis of differential centrifugation fractions from native inner medullary collecting duct of rat
    Article Snippet: .. Peptides recovered from gel slices were injected into a reverse-phase liquid chromatographic (LC) column (PicoFritTM, Biobasic C18, New Objective, Woodburn, MA) for further separation, and then delivered into an LTQ tandem mass spectrometer (Thermo Electron, San Jose, CA) via electrospray. ..

    Article Title: Response and Adaptation of Escherichia coli to Suppression of the Amber Stop Codon
    Article Snippet: .. Automated 2D nanoflow LC-MS/MS analysis was performed using LTQ tandem mass spectrometer (Thermo Electron Corporation) employing automated data-dependent acquisition. .. An Agilent 1100 HPLC system was used to deliver a flow rate of 300 nL min−1 to the mass spectrometer through a splitter.

    Article Title: Proteomic analysis of aqueous humor from patients with myopia
    Article Snippet: .. Liquid chromatography/mass spectrometry/mass spectrometry (LC MS/MS) analysis was performed in an LTQ tandem mass spectrometer (ThermoFinnigan, San Jose, CA) coupled online with an HPLC system. .. Protein digests obtained above were loaded on the HPLC, which was connected with an in-house made fused silica capillary column (10-cm-length; 100 µm inner diameter) and packed with sunchrom 5 μm C18 resin.

    Article Title: RNA Helicase p68 (DDX5) Regulates tau Exon 10 Splicing by Modulating a Stem-Loop Structure at the 5? Splice Site ▿
    Article Snippet: .. The eluted peptides were ionized and directly sprayed into an LTQ tandem mass spectrometer (ThermoFisher Scientific). ..

    Article Title: UV Irradiation Accelerates Amyloid Precursor Protein (APP) Processing and Disrupts APP Axonal Transport
    Article Snippet: .. Automated 2D nanoflow LC-MS/MS analysis was performed using LTQ tandem mass spectrometer (Thermo Electron) using automated data-dependent acquisition. ..

    Article Title: Proteomic analysis of V-ATPase-rich cells harvested from the kidney and epididymis by fluorescence-activated cell sorting
    Article Snippet: .. Tryptic peptides were stratified over time by reverse-phase LC (PicoFrit, BioBasic C18 column, New Objective, Woburn, MA) before delivery to an LTQ tandem mass spectrometer (MS/MS, Thermo Fisher Scientific) equipped with a nanoelectrospray ion source. .. The mass-to-charge ( m / z ) ratios of peptides and their fragmented ions were recorded as spectra by the mass spectrometer.

    Article Title: Large-scale quantitative LC-MS/MS analysis of detergent-resistant membrane proteins from rat renal collecting duct
    Article Snippet: .. Tryptic peptides extracted from each gel slice were injected into a reverse-phase LC column (PicoFrit, Biobasic C18, New Objective, Woodburn, MA) to stratify sample proteins before delivery to an LTQ tandem mass spectrometer (MS/MS, Thermo Electron, San Jose, CA) via a nanoelectrospray ion source. .. The spectra with a total ion current > 10,000 were used to search for matches to peptides in a concatenated RefSeq database using Bioworks software (version 3.1, Thermo Electron) based on the Sequest algorithm.

    Chromatography:

    Article Title: Proteomic analysis of aqueous humor from patients with myopia
    Article Snippet: .. Liquid chromatography/mass spectrometry/mass spectrometry (LC MS/MS) analysis was performed in an LTQ tandem mass spectrometer (ThermoFinnigan, San Jose, CA) coupled online with an HPLC system. .. Protein digests obtained above were loaded on the HPLC, which was connected with an in-house made fused silica capillary column (10-cm-length; 100 µm inner diameter) and packed with sunchrom 5 μm C18 resin.

    Tandem Mass Spectroscopy:

    Article Title: Proteomic analysis of aqueous humor from patients with myopia
    Article Snippet: .. Liquid chromatography/mass spectrometry/mass spectrometry (LC MS/MS) analysis was performed in an LTQ tandem mass spectrometer (ThermoFinnigan, San Jose, CA) coupled online with an HPLC system. .. Protein digests obtained above were loaded on the HPLC, which was connected with an in-house made fused silica capillary column (10-cm-length; 100 µm inner diameter) and packed with sunchrom 5 μm C18 resin.

    Article Title: Proteomic analysis of V-ATPase-rich cells harvested from the kidney and epididymis by fluorescence-activated cell sorting
    Article Snippet: .. Tryptic peptides were stratified over time by reverse-phase LC (PicoFrit, BioBasic C18 column, New Objective, Woburn, MA) before delivery to an LTQ tandem mass spectrometer (MS/MS, Thermo Fisher Scientific) equipped with a nanoelectrospray ion source. .. The mass-to-charge ( m / z ) ratios of peptides and their fragmented ions were recorded as spectra by the mass spectrometer.

    Article Title: Large-scale quantitative LC-MS/MS analysis of detergent-resistant membrane proteins from rat renal collecting duct
    Article Snippet: .. Tryptic peptides extracted from each gel slice were injected into a reverse-phase LC column (PicoFrit, Biobasic C18, New Objective, Woodburn, MA) to stratify sample proteins before delivery to an LTQ tandem mass spectrometer (MS/MS, Thermo Electron, San Jose, CA) via a nanoelectrospray ion source. .. The spectra with a total ion current > 10,000 were used to search for matches to peptides in a concatenated RefSeq database using Bioworks software (version 3.1, Thermo Electron) based on the Sequest algorithm.

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    Thermo Fisher ltq orbitrap xl mass spectrometer
    Flowchart of pseudo triplex labeling approach Tryptic digests of NS control sample were loaded onto the SPE column and then flushed with the light labeling reagent. After washed, tryptic digest of NgBR knockdown sample were loaded onto the same SPE column and then were labeled with the intermediate labeling reagent. After washed, the same amount of tryptic digests of NS control sample were loaded onto the column again and labeled with the heavy labeling reagent. The labeled sample mixture was eluted with 500 μL × 2 of 80% ACN containing 5% ammonia solution and dried by the vacuum centrifugation. The lyophilized samples were first resuspended and was injected automated onto the SCX trap column. Then, a series stepwise elution with salt concentrations of 50, 100, 150, 200, 250, 300, 350, 400, 500 and 1000 mM NH 4 AC (pH 2.7) was utilized to gradually elute peptides from SCX trap column to the C18 separation column. Finally, a 90 min binary RP gradient nano-RPLC MS/MS analysis was applied to separate peptides prior to mass spectrometry detection. The <t>LTQ-Orbitrap</t> XL mass spectrometer (Thermo Scientific, San Jose, CA, USA) was operated in a data dependent MS/MS acquisition mode. System controlling and data collection were carried out by Xcalibur software version 2.0.7 (Thermo Scientific).
    Ltq Orbitrap Xl Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 436 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltq orbitrap xl mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 436 article reviews
    Price from $9.99 to $1999.99
    ltq orbitrap xl mass spectrometer - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    88
    Thermo Fisher esi ltq orbitrap
    (A) Expansion of the m/z 750-1,000 region in the <t>Orbitrap-ESI</t> mass spectra for T. suecica with MS/MS verification. All species are lithiated adducts. (B) Inset represents tandem MS used to elucidate fatty acid composition of each TAG. Fatty Acids: P =
    Esi Ltq Orbitrap, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/esi ltq orbitrap/product/Thermo Fisher
    Average 88 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    esi ltq orbitrap - by Bioz Stars, 2020-05
    88/100 stars
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    93
    Thermo Fisher ltq orbitrap velos mass spectrometer
    Overview of phosphoproteome upon palmella formation of Dunaliella salina . (A) Overlap of phosphorylation sites among three biological replicates. All phosphorylated sites having a reported localization probability of at least 0.75 were considered to be assigned to a specific residue, and we refer to these as phosphorylated sites. 137 phosphorylated sites were identified. (B) Among the 137 identified sites, 54 phosphorylated sites were quantified. Three biological replicates for each sample were performed by <t>LTQ-Orbitrap</t> <t>Velos</t> MS/MS analysis, and the obtained results were given in Supplemental Table S3 . We compared the overlap of identified phosphorylated sites and quantified phosphorylated sites among the three biological replicates. Biol.1/2/3 represent the first/second/third biological replicate. (C) Functional category of 100 identified phosphoproteins (containing 137 identified phosphorylated sites) in D. salina . The numbers inside the parentheses shows how many phosphorylated sites identified in phosphoproteins. (D) Functional category of 40 quantified phosphoproteins (containing 54 quantified phosphorylated sites). The numbers inside the parentheses show how many phosphorylated sites quantified in phosphoproteins. (E) A representative MS/MS spectra of 35 salinity-responsive phosphoproteins. The peptide GYLTDLK from PRP1 splicing factor (gi|307110542).
    Ltq Orbitrap Velos Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltq orbitrap velos mass spectrometer/product/Thermo Fisher
    Average 93 stars, based on 81 article reviews
    Price from $9.99 to $1999.99
    ltq orbitrap velos mass spectrometer - by Bioz Stars, 2020-05
    93/100 stars
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    Flowchart of pseudo triplex labeling approach Tryptic digests of NS control sample were loaded onto the SPE column and then flushed with the light labeling reagent. After washed, tryptic digest of NgBR knockdown sample were loaded onto the same SPE column and then were labeled with the intermediate labeling reagent. After washed, the same amount of tryptic digests of NS control sample were loaded onto the column again and labeled with the heavy labeling reagent. The labeled sample mixture was eluted with 500 μL × 2 of 80% ACN containing 5% ammonia solution and dried by the vacuum centrifugation. The lyophilized samples were first resuspended and was injected automated onto the SCX trap column. Then, a series stepwise elution with salt concentrations of 50, 100, 150, 200, 250, 300, 350, 400, 500 and 1000 mM NH 4 AC (pH 2.7) was utilized to gradually elute peptides from SCX trap column to the C18 separation column. Finally, a 90 min binary RP gradient nano-RPLC MS/MS analysis was applied to separate peptides prior to mass spectrometry detection. The LTQ-Orbitrap XL mass spectrometer (Thermo Scientific, San Jose, CA, USA) was operated in a data dependent MS/MS acquisition mode. System controlling and data collection were carried out by Xcalibur software version 2.0.7 (Thermo Scientific).

    Journal: Journal of proteomics

    Article Title: Comprehensive proteome quantification reveals NgBR as a new regulator for Epithelial-Mesenchymal Transition of breast tumor cells

    doi: 10.1016/j.jprot.2014.08.007

    Figure Lengend Snippet: Flowchart of pseudo triplex labeling approach Tryptic digests of NS control sample were loaded onto the SPE column and then flushed with the light labeling reagent. After washed, tryptic digest of NgBR knockdown sample were loaded onto the same SPE column and then were labeled with the intermediate labeling reagent. After washed, the same amount of tryptic digests of NS control sample were loaded onto the column again and labeled with the heavy labeling reagent. The labeled sample mixture was eluted with 500 μL × 2 of 80% ACN containing 5% ammonia solution and dried by the vacuum centrifugation. The lyophilized samples were first resuspended and was injected automated onto the SCX trap column. Then, a series stepwise elution with salt concentrations of 50, 100, 150, 200, 250, 300, 350, 400, 500 and 1000 mM NH 4 AC (pH 2.7) was utilized to gradually elute peptides from SCX trap column to the C18 separation column. Finally, a 90 min binary RP gradient nano-RPLC MS/MS analysis was applied to separate peptides prior to mass spectrometry detection. The LTQ-Orbitrap XL mass spectrometer (Thermo Scientific, San Jose, CA, USA) was operated in a data dependent MS/MS acquisition mode. System controlling and data collection were carried out by Xcalibur software version 2.0.7 (Thermo Scientific).

    Article Snippet: The LTQ-Orbitrap XL mass spectrometer (Thermo Scientific, San Jose, CA, USA) was operated in a data dependent MS/MS acquisition mode.

    Techniques: Labeling, Centrifugation, Injection, Mass Spectrometry, Software

    Chromatogram of LC–MS/MS analysis of tryptic peptides from normal pancreatic duct and PANC-1 cells. Twenty microgram tryptic peptides and 100 fmol standard peptide Ang I were loaded to C 18 capillary column, and the eluted peptides were analyzed by LTQ-Orbitrap. For the first iteration of normal duct sample, the retention time of exogenous control Ang I was 78 min, and the retention times of 4 internal control peptides were 26 min, 42 min, 56 min, and 91 min respectively. Labeled peak A is the peptide IWHHTFYNELR (2+ ion, m / z 758.3766) from actin, gamma 1; peak B is the peptide VAPEEHPVLLTEAPLNPK (2+ ion, m / z 977.5364) from actin, gamma 1; peak C is the peptide LGEHNIDVLEGNEQFINAAK (2+ ion, m / z 1106.0545) from trypsin autolysis; peak D is the peptide LCYVALDFEQEMATAASSSSLEK (2+ ion, m / z 1275.5953) from actin, gamma 1. The mass accuracy of these peptides was within 5 ppm. These peptides were also identified in the second and third iterations of normal duct sample, and in 3 iterations of PANC-1 sample with similar retention times (±2.0 min), indicating that the experimental procedure was reproducible and the MS results could be compared.

    Journal: Journal of proteome research

    Article Title: Proteomic Analysis Reveals Warburg Effect and Anomalous Metabolism of Glutamine in Pancreatic Cancer Cells

    doi: 10.1021/pr2009274

    Figure Lengend Snippet: Chromatogram of LC–MS/MS analysis of tryptic peptides from normal pancreatic duct and PANC-1 cells. Twenty microgram tryptic peptides and 100 fmol standard peptide Ang I were loaded to C 18 capillary column, and the eluted peptides were analyzed by LTQ-Orbitrap. For the first iteration of normal duct sample, the retention time of exogenous control Ang I was 78 min, and the retention times of 4 internal control peptides were 26 min, 42 min, 56 min, and 91 min respectively. Labeled peak A is the peptide IWHHTFYNELR (2+ ion, m / z 758.3766) from actin, gamma 1; peak B is the peptide VAPEEHPVLLTEAPLNPK (2+ ion, m / z 977.5364) from actin, gamma 1; peak C is the peptide LGEHNIDVLEGNEQFINAAK (2+ ion, m / z 1106.0545) from trypsin autolysis; peak D is the peptide LCYVALDFEQEMATAASSSSLEK (2+ ion, m / z 1275.5953) from actin, gamma 1. The mass accuracy of these peptides was within 5 ppm. These peptides were also identified in the second and third iterations of normal duct sample, and in 3 iterations of PANC-1 sample with similar retention times (±2.0 min), indicating that the experimental procedure was reproducible and the MS results could be compared.

    Article Snippet: The LTQ-Orbitrap mass spectrometer was operated in a data-dependent mode in which each full MS scan (60000 resolving power) was followed by eight MS/MS scans where the eight most abundant molecular ions were dynamically selected and fragmented by collision-induced dissociation (CID) using a normalized collision energy of 35%.

    Techniques: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Labeling

    (A) Expansion of the m/z 750-1,000 region in the Orbitrap-ESI mass spectra for T. suecica with MS/MS verification. All species are lithiated adducts. (B) Inset represents tandem MS used to elucidate fatty acid composition of each TAG. Fatty Acids: P =

    Journal: Journal of Lipid Research

    Article Title: Triacylglycerol profiling of marine microalgae by mass spectrometry [S]

    doi: 10.1194/jlr.D018408

    Figure Lengend Snippet: (A) Expansion of the m/z 750-1,000 region in the Orbitrap-ESI mass spectra for T. suecica with MS/MS verification. All species are lithiated adducts. (B) Inset represents tandem MS used to elucidate fatty acid composition of each TAG. Fatty Acids: P =

    Article Snippet: Tandem MS using both ESI-LTQ-Orbitrap and MALDI-TOF allowed us to correctly identify the specific fatty acid composition in TAG peaks (supplementary Table I).

    Techniques: Mass Spectrometry

    Overview of phosphoproteome upon palmella formation of Dunaliella salina . (A) Overlap of phosphorylation sites among three biological replicates. All phosphorylated sites having a reported localization probability of at least 0.75 were considered to be assigned to a specific residue, and we refer to these as phosphorylated sites. 137 phosphorylated sites were identified. (B) Among the 137 identified sites, 54 phosphorylated sites were quantified. Three biological replicates for each sample were performed by LTQ-Orbitrap Velos MS/MS analysis, and the obtained results were given in Supplemental Table S3 . We compared the overlap of identified phosphorylated sites and quantified phosphorylated sites among the three biological replicates. Biol.1/2/3 represent the first/second/third biological replicate. (C) Functional category of 100 identified phosphoproteins (containing 137 identified phosphorylated sites) in D. salina . The numbers inside the parentheses shows how many phosphorylated sites identified in phosphoproteins. (D) Functional category of 40 quantified phosphoproteins (containing 54 quantified phosphorylated sites). The numbers inside the parentheses show how many phosphorylated sites quantified in phosphoproteins. (E) A representative MS/MS spectra of 35 salinity-responsive phosphoproteins. The peptide GYLTDLK from PRP1 splicing factor (gi|307110542).

    Journal: Frontiers in Plant Science

    Article Title: Salinity-Induced Palmella Formation Mechanism in Halotolerant Algae Dunaliella salina Revealed by Quantitative Proteomics and Phosphoproteomics

    doi: 10.3389/fpls.2017.00810

    Figure Lengend Snippet: Overview of phosphoproteome upon palmella formation of Dunaliella salina . (A) Overlap of phosphorylation sites among three biological replicates. All phosphorylated sites having a reported localization probability of at least 0.75 were considered to be assigned to a specific residue, and we refer to these as phosphorylated sites. 137 phosphorylated sites were identified. (B) Among the 137 identified sites, 54 phosphorylated sites were quantified. Three biological replicates for each sample were performed by LTQ-Orbitrap Velos MS/MS analysis, and the obtained results were given in Supplemental Table S3 . We compared the overlap of identified phosphorylated sites and quantified phosphorylated sites among the three biological replicates. Biol.1/2/3 represent the first/second/third biological replicate. (C) Functional category of 100 identified phosphoproteins (containing 137 identified phosphorylated sites) in D. salina . The numbers inside the parentheses shows how many phosphorylated sites identified in phosphoproteins. (D) Functional category of 40 quantified phosphoproteins (containing 54 quantified phosphorylated sites). The numbers inside the parentheses show how many phosphorylated sites quantified in phosphoproteins. (E) A representative MS/MS spectra of 35 salinity-responsive phosphoproteins. The peptide GYLTDLK from PRP1 splicing factor (gi|307110542).

    Article Snippet: The LTQ-Orbitrap Velos mass spectrometer (Thermo Fisher Scientific) was operated in data-dependent MS/MS acquisition mode.

    Techniques: Mass Spectrometry, Functional Assay