ltq orbitrapxl hybrid ion trap orbitrap mass spectrometer  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    LTQ Orbitrap XL Hybrid Ion Trap Orbitrap Mass Spectrometer
    Description:
    Perform a range of applications from protein identification to biomarker discovery de novo sequencing and metabolite profiling with confidence The Thermo Scientific LTQ Orbitrap XL Hybrid Ion Trap Orbitrap Mass Spectrometer is a Fourier Transform Mass Spectrometer FTMS based on Thermo Scientific LTQ XL linear ion trap and Orbitrap mass spectrometer technologies To enhance the flexibility of fragmentation experiments for advanced proteomics and small molecule research the instrument features an HCD collision cell
    Catalog Number:
    iqlaaegaapfadbmaok
    Price:
    None
    Applications:
    Industrial & Applied Science|Industrial Mass Spectrometry
    Category:
    Instruments and Equipment
    Buy from Supplier


    Structured Review

    Thermo Fisher ltq orbitrapxl hybrid ion trap orbitrap mass spectrometer
    Perform a range of applications from protein identification to biomarker discovery de novo sequencing and metabolite profiling with confidence The Thermo Scientific LTQ Orbitrap XL Hybrid Ion Trap Orbitrap Mass Spectrometer is a Fourier Transform Mass Spectrometer FTMS based on Thermo Scientific LTQ XL linear ion trap and Orbitrap mass spectrometer technologies To enhance the flexibility of fragmentation experiments for advanced proteomics and small molecule research the instrument features an HCD collision cell
    https://www.bioz.com/result/ltq orbitrapxl hybrid ion trap orbitrap mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ltq orbitrapxl hybrid ion trap orbitrap mass spectrometer - by Bioz Stars, 2020-07
    99/100 stars

    Images

    Related Articles

    High Performance Liquid Chromatography:

    Article Title: 14-3-3 binding to LRRK2 is disrupted by multiple Parkinson's disease-associated mutations and regulates cytoplasmic localization
    Article Snippet: .. The HPLC system was coupled to a linear ion-trap–orbitrap hybrid mass spectrometer (LTQ-Orbitrap XL, Thermo Fisher Scientific) via a nanoelectrospray ion source (Proxeon Biosystems) fitted with a 5 cm Picotip FS360-20-10 emitter. ..

    Article Title: Quantitative Proteomic Discovery of Dynamic Epigenome Changes that Control Human Cytomegalovirus (HCMV) Infection *
    Article Snippet: .. The HPLC effluent was directly coupled to an LTQ-Orbitrap XL hybrid mass spectrometer (ThermoFisher Scientific, San Jose, CA) with a resolution of 30,000 for full MS followed by seven data-dependent and targeted MS/MS acquisitions. .. To quantify RNA levels of DOT1L, total cellular RNA was isolated using Tri-Reagent (Sigma). cDNA was generated using 1 μg of RNA with TaqMan Reverse Transcription Reagents (Roche), and equal volumes of cDNA was utilized for quantitative PCR (qPCR).

    Quantitation Assay:

    Article Title: Investigation of Age-Specific Behavioral and Proteomic Changes in an Animal Model of Chronic Ethanol Exposure
    Article Snippet: .. Mass spectrometric analysis for iTRAQ-based quantitation is carried out on a hybrid linear ion trap-Orbitrap mass spectrometer (LTQ Orbitrap XL, Thermo Fisher Scientific) ( see Note ). .. Several data analysis software packages can be used for processing of mass spectrometric data for relative protein quantitation by either spectral counting or iTRAQ.

    Mass Spectrometry:

    Article Title: O-Glycosylation Modulates Proprotein Convertase Activation of Angiopoietin-like Protein 3
    Article Snippet: .. The product of ANGPTL3 dodecapeptide with GalNAc-T2 was characterized by electrospray ionization-linear ion trap-Fourier transform mass spectrometry in an LTQ-Orbitrap XL hybrid spectrometer (Thermo-Scientific, Bremen, Germany), equipped with electron transfer dissociation (ETD) for peptide sequence analysis by MS/MS (MS2 ) with retention of glycan site-specific fragments. .. The sample was dissolved in methanol/water (1:1) containing 1% formic acid and introduced by direct infusion via a TriVersa NanoMate ESI-Chip interface (Advion BioSystems, Ithaca, NY), at a flow rate of ∼100 nl/min and 1.4 kV spray voltage.

    Article Title: Investigation of Age-Specific Behavioral and Proteomic Changes in an Animal Model of Chronic Ethanol Exposure
    Article Snippet: .. Mass spectrometric analysis for iTRAQ-based quantitation is carried out on a hybrid linear ion trap-Orbitrap mass spectrometer (LTQ Orbitrap XL, Thermo Fisher Scientific) ( see Note ). .. Several data analysis software packages can be used for processing of mass spectrometric data for relative protein quantitation by either spectral counting or iTRAQ.

    Article Title: Lectin Domains of Polypeptide GalNAc Transferases Exhibit Glycopeptide Binding Specificity *
    Article Snippet: .. Characterization of O-Glycosylation Sites Products of O -glycosylation reactions were characterized by electrospray ionization-linear ion trap-Fourier transform mass spectrometry (ESI-LIT-FT-MS) in an LTQ-Orbitrap XL hybrid spectrometer (Thermo-Scientific) equipped for both high energy collision-induced dissociation ( ) in an external collision cell , and electron transfer dissociation (ETD) ( ) for peptide sequence analysis by MS/MS (MS2 ) with retention of glycan site-specific fragments. .. Samples were dissolved in methanol/water (1:1) containing 1% formic acid and introduced by direct infusion via a TriVersa NanoMate ESI-Chip interface (Advion BioSystems) at a flow rate of ∼100 nl/min and 1.4 kV spray voltage.

    Article Title: 14-3-3 binding to LRRK2 is disrupted by multiple Parkinson's disease-associated mutations and regulates cytoplasmic localization
    Article Snippet: .. The HPLC system was coupled to a linear ion-trap–orbitrap hybrid mass spectrometer (LTQ-Orbitrap XL, Thermo Fisher Scientific) via a nanoelectrospray ion source (Proxeon Biosystems) fitted with a 5 cm Picotip FS360-20-10 emitter. ..

    Article Title: An Internal Standard-Assisted Synthesis and Degradation Proteomic Approach Reveals the Potential Linkage between VPS4B Depletion and Activation of Fatty Acid β-Oxidation in Breast Cancer Cells
    Article Snippet: .. LC-MS/MS Analysis Peptides were dissolved in 0.5% acetic acid (solvent A) and separated on a 10 cm reverse-phase PicoFrit spray tip (New Objective, Woburn, MA) packed in house with sub-2 μ m C18 resin (Prospereon Life Science, IL), using a nanoflow Xtreme simple liquid chromatography system (Microtech/CVC) coupled to a hybrid linear ion trap-Orbitrap mass spectrometer (LTQ Orbitrap, Thermo Scientific). .. Peptides were loaded onto the column with solvent A at a flow rate of 0.6 μ L/min and eluted with a 180 min linear gradient at a flow rate of 0.2 μ L/min.

    Article Title: Quantitative HPLC-MS analysis of nucleotide sugars in plant cells following off-line SPE sample preparation
    Article Snippet: .. Fragmentation experiments were performed on a linear ion trap–Orbitrap mass spectrometer (Model LTQ Orbitrap XL, Thermo Fisher Scientific). .. Establishing stable retention times for UDP-sugars on PGC Figure illustrates the effect of the electrical potential applied to the ESI source on chromatographic retention of UDP-sugars on PGC.

    Article Title: Quantitative Proteomic Discovery of Dynamic Epigenome Changes that Control Human Cytomegalovirus (HCMV) Infection *
    Article Snippet: .. The HPLC effluent was directly coupled to an LTQ-Orbitrap XL hybrid mass spectrometer (ThermoFisher Scientific, San Jose, CA) with a resolution of 30,000 for full MS followed by seven data-dependent and targeted MS/MS acquisitions. .. To quantify RNA levels of DOT1L, total cellular RNA was isolated using Tri-Reagent (Sigma). cDNA was generated using 1 μg of RNA with TaqMan Reverse Transcription Reagents (Roche), and equal volumes of cDNA was utilized for quantitative PCR (qPCR).

    Tandem Mass Spectroscopy:

    Article Title: O-Glycosylation Modulates Proprotein Convertase Activation of Angiopoietin-like Protein 3
    Article Snippet: .. The product of ANGPTL3 dodecapeptide with GalNAc-T2 was characterized by electrospray ionization-linear ion trap-Fourier transform mass spectrometry in an LTQ-Orbitrap XL hybrid spectrometer (Thermo-Scientific, Bremen, Germany), equipped with electron transfer dissociation (ETD) for peptide sequence analysis by MS/MS (MS2 ) with retention of glycan site-specific fragments. .. The sample was dissolved in methanol/water (1:1) containing 1% formic acid and introduced by direct infusion via a TriVersa NanoMate ESI-Chip interface (Advion BioSystems, Ithaca, NY), at a flow rate of ∼100 nl/min and 1.4 kV spray voltage.

    Article Title: Lectin Domains of Polypeptide GalNAc Transferases Exhibit Glycopeptide Binding Specificity *
    Article Snippet: .. Characterization of O-Glycosylation Sites Products of O -glycosylation reactions were characterized by electrospray ionization-linear ion trap-Fourier transform mass spectrometry (ESI-LIT-FT-MS) in an LTQ-Orbitrap XL hybrid spectrometer (Thermo-Scientific) equipped for both high energy collision-induced dissociation ( ) in an external collision cell , and electron transfer dissociation (ETD) ( ) for peptide sequence analysis by MS/MS (MS2 ) with retention of glycan site-specific fragments. .. Samples were dissolved in methanol/water (1:1) containing 1% formic acid and introduced by direct infusion via a TriVersa NanoMate ESI-Chip interface (Advion BioSystems) at a flow rate of ∼100 nl/min and 1.4 kV spray voltage.

    Article Title: Quantitative Proteomic Discovery of Dynamic Epigenome Changes that Control Human Cytomegalovirus (HCMV) Infection *
    Article Snippet: .. The HPLC effluent was directly coupled to an LTQ-Orbitrap XL hybrid mass spectrometer (ThermoFisher Scientific, San Jose, CA) with a resolution of 30,000 for full MS followed by seven data-dependent and targeted MS/MS acquisitions. .. To quantify RNA levels of DOT1L, total cellular RNA was isolated using Tri-Reagent (Sigma). cDNA was generated using 1 μg of RNA with TaqMan Reverse Transcription Reagents (Roche), and equal volumes of cDNA was utilized for quantitative PCR (qPCR).

    Sequencing:

    Article Title: O-Glycosylation Modulates Proprotein Convertase Activation of Angiopoietin-like Protein 3
    Article Snippet: .. The product of ANGPTL3 dodecapeptide with GalNAc-T2 was characterized by electrospray ionization-linear ion trap-Fourier transform mass spectrometry in an LTQ-Orbitrap XL hybrid spectrometer (Thermo-Scientific, Bremen, Germany), equipped with electron transfer dissociation (ETD) for peptide sequence analysis by MS/MS (MS2 ) with retention of glycan site-specific fragments. .. The sample was dissolved in methanol/water (1:1) containing 1% formic acid and introduced by direct infusion via a TriVersa NanoMate ESI-Chip interface (Advion BioSystems, Ithaca, NY), at a flow rate of ∼100 nl/min and 1.4 kV spray voltage.

    Article Title: Lectin Domains of Polypeptide GalNAc Transferases Exhibit Glycopeptide Binding Specificity *
    Article Snippet: .. Characterization of O-Glycosylation Sites Products of O -glycosylation reactions were characterized by electrospray ionization-linear ion trap-Fourier transform mass spectrometry (ESI-LIT-FT-MS) in an LTQ-Orbitrap XL hybrid spectrometer (Thermo-Scientific) equipped for both high energy collision-induced dissociation ( ) in an external collision cell , and electron transfer dissociation (ETD) ( ) for peptide sequence analysis by MS/MS (MS2 ) with retention of glycan site-specific fragments. .. Samples were dissolved in methanol/water (1:1) containing 1% formic acid and introduced by direct infusion via a TriVersa NanoMate ESI-Chip interface (Advion BioSystems) at a flow rate of ∼100 nl/min and 1.4 kV spray voltage.

    Liquid Chromatography:

    Article Title: An Internal Standard-Assisted Synthesis and Degradation Proteomic Approach Reveals the Potential Linkage between VPS4B Depletion and Activation of Fatty Acid β-Oxidation in Breast Cancer Cells
    Article Snippet: .. LC-MS/MS Analysis Peptides were dissolved in 0.5% acetic acid (solvent A) and separated on a 10 cm reverse-phase PicoFrit spray tip (New Objective, Woburn, MA) packed in house with sub-2 μ m C18 resin (Prospereon Life Science, IL), using a nanoflow Xtreme simple liquid chromatography system (Microtech/CVC) coupled to a hybrid linear ion trap-Orbitrap mass spectrometer (LTQ Orbitrap, Thermo Scientific). .. Peptides were loaded onto the column with solvent A at a flow rate of 0.6 μ L/min and eluted with a 180 min linear gradient at a flow rate of 0.2 μ L/min.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher maldi linear ion trap orbitrap hybrid mass spectrometer
    Accumulation of lipid droplets and spatial distribution of different triacylglycerol (TAG) molecular species within transgenic leaf tissue. (a) 3D reconstructed z-stacks of confocal images from wild-type (left) and transgenic (right) leaf tissue. Neutral lipids including TAG, stained with BODIPY, appear as green droplets within the leaf mesophyll cells that contain visible chloroplasts (red autofluorescence). Note that non-TAG features also fluorescing green include the vascular bundle and leaf epidermis. (b) Bright-field microscopy images of wild-type (upper) and transgenic (lower) leaf cross-sections subsequently used for <t>MALDI-Orbitrap</t> imaging. Scale bars correspond to 1000 microns. (c) Ratio of TAG total ion counts (TIC; all TAG molecular species summed at each analysed position) to PC-TIC as detected by MALDI-Orbitrap imaging in wild-type (upper) and transgenic (lower) leaf cross-sections. (d) Spatial distribution of selected dominant TAG and PC species across a transgenic leaf cross-section as observed by MALDI-Orbitrap.
    Maldi Linear Ion Trap Orbitrap Hybrid Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/maldi linear ion trap orbitrap hybrid mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 144 article reviews
    Price from $9.99 to $1999.99
    maldi linear ion trap orbitrap hybrid mass spectrometer - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    Accumulation of lipid droplets and spatial distribution of different triacylglycerol (TAG) molecular species within transgenic leaf tissue. (a) 3D reconstructed z-stacks of confocal images from wild-type (left) and transgenic (right) leaf tissue. Neutral lipids including TAG, stained with BODIPY, appear as green droplets within the leaf mesophyll cells that contain visible chloroplasts (red autofluorescence). Note that non-TAG features also fluorescing green include the vascular bundle and leaf epidermis. (b) Bright-field microscopy images of wild-type (upper) and transgenic (lower) leaf cross-sections subsequently used for MALDI-Orbitrap imaging. Scale bars correspond to 1000 microns. (c) Ratio of TAG total ion counts (TIC; all TAG molecular species summed at each analysed position) to PC-TIC as detected by MALDI-Orbitrap imaging in wild-type (upper) and transgenic (lower) leaf cross-sections. (d) Spatial distribution of selected dominant TAG and PC species across a transgenic leaf cross-section as observed by MALDI-Orbitrap.

    Journal: Plant Biotechnology Journal

    Article Title: Metabolic engineering of biomass for high energy density: oilseed-like triacylglycerol yields from plant leaves

    doi: 10.1111/pbi.12131

    Figure Lengend Snippet: Accumulation of lipid droplets and spatial distribution of different triacylglycerol (TAG) molecular species within transgenic leaf tissue. (a) 3D reconstructed z-stacks of confocal images from wild-type (left) and transgenic (right) leaf tissue. Neutral lipids including TAG, stained with BODIPY, appear as green droplets within the leaf mesophyll cells that contain visible chloroplasts (red autofluorescence). Note that non-TAG features also fluorescing green include the vascular bundle and leaf epidermis. (b) Bright-field microscopy images of wild-type (upper) and transgenic (lower) leaf cross-sections subsequently used for MALDI-Orbitrap imaging. Scale bars correspond to 1000 microns. (c) Ratio of TAG total ion counts (TIC; all TAG molecular species summed at each analysed position) to PC-TIC as detected by MALDI-Orbitrap imaging in wild-type (upper) and transgenic (lower) leaf cross-sections. (d) Spatial distribution of selected dominant TAG and PC species across a transgenic leaf cross-section as observed by MALDI-Orbitrap.

    Article Snippet: Sections were scanned at 25 micron step-size in a MALDI linear ion-trap-Orbitrap hybrid mass spectrometer (MALDI LTQ Orbitrap-XL; Thermo Fisher Scientific, Bremen, Germany).

    Techniques: Transgenic Assay, Staining, Microscopy, Imaging

    Influence of the potential applied to the ESI source on retention of UDP-sugars. Chromatogram obtained with a newly installed or regenerated PGC column ( a ); chromatogram obtained after 20-min utilization of the column connected to the ESI source of an Orbitrap mass spectrometer ( b ). Conditions: column regeneration was performed with 80 % acetonitrile in water containing 0.10 % trifluoroacetic acid for 20 min, the sample measurements were done with the gradient program described in the materials and methods section for HPLC-MS; detection, UV, 262 nm; sample. UDP-Gal (100 μmol L −1 ) and UDP-Glc (100 μmol L −1 )

    Journal: Analytical and Bioanalytical Chemistry

    Article Title: Quantitative HPLC-MS analysis of nucleotide sugars in plant cells following off-line SPE sample preparation

    doi: 10.1007/s00216-014-7746-3

    Figure Lengend Snippet: Influence of the potential applied to the ESI source on retention of UDP-sugars. Chromatogram obtained with a newly installed or regenerated PGC column ( a ); chromatogram obtained after 20-min utilization of the column connected to the ESI source of an Orbitrap mass spectrometer ( b ). Conditions: column regeneration was performed with 80 % acetonitrile in water containing 0.10 % trifluoroacetic acid for 20 min, the sample measurements were done with the gradient program described in the materials and methods section for HPLC-MS; detection, UV, 262 nm; sample. UDP-Gal (100 μmol L −1 ) and UDP-Glc (100 μmol L −1 )

    Article Snippet: Fragmentation experiments were performed on a linear ion trap–Orbitrap mass spectrometer (Model LTQ Orbitrap XL, Thermo Fisher Scientific).

    Techniques: Pyrolysis Gas Chromatography, Mass Spectrometry, High Performance Liquid Chromatography, Gas Chromatography

    Quality control after cross-link identification at a 5% link FDR. (a) Results from cross-linking HSA with sulfo-SDA acquired on an Q Exactive and an LTQ Orbitrap Velos mass spectrometer. The line at 25 Å indicates the distance cutoff for links classified as long distance. The inlet shows the fraction of long-distance links (LDL) in each data set. (b) Score comparison between within-distance linkzs and LDL. LDL showed no significant (ns) deviation from the within-distance links (two-sided Mann–Whitney-U-test at α = 0.05).

    Journal: Analytical Chemistry

    Article Title: Noncovalently Associated Peptides Observed during Liquid Chromatography-Mass Spectrometry and Their Effect on Cross-Link Analyses

    doi: 10.1021/acs.analchem.8b04037

    Figure Lengend Snippet: Quality control after cross-link identification at a 5% link FDR. (a) Results from cross-linking HSA with sulfo-SDA acquired on an Q Exactive and an LTQ Orbitrap Velos mass spectrometer. The line at 25 Å indicates the distance cutoff for links classified as long distance. The inlet shows the fraction of long-distance links (LDL) in each data set. (b) Score comparison between within-distance linkzs and LDL. LDL showed no significant (ns) deviation from the within-distance links (two-sided Mann–Whitney-U-test at α = 0.05).

    Article Snippet: We observed surprising differences when comparing the identified cross-links using data acquired on two different mass spectrometers: a hybrid linear ion trap-Orbitrap mass spectrometer (LTQ Orbitrap Velos, Thermo Fisher Scientific) and a hybrid quadrupole-Orbitrap mass spectrometer (Q Exactive, Thermo Fisher Scientific).

    Techniques: Mass Spectrometry, MANN-WHITNEY

    Using internal standard-assisted synthesis and degradation mass spectrometry (iSDMS) to study the roles of EGF on global protein synthesis and degradation. SKBR3_shVPS4B and the parental SKBR3 cells were first labeled with 13 C 6 -arginine (Arg6) and D 4 -lysine (Lys4) medium (labeled in red). After overnight serum starvation, Arg6/Lys4-labeled cells were stimulated with 100 ng/mL EGF in medium supplemented with regular arginine (Arg0) and lysine (Lys0) for 0, 2, 6, 12, and 24 hr (labeled in blue). Protein isolated from SKBR3_shVPS4B cells labeled with 13 C 6 15 N 4 -arginine (Arg10) and 13 C 6 15 N 2 -lysine (Lys8)—internal standard (labeled in green)—was spiked into each sample at a ratio of 1 : 3 (wt/wt). The mixtures were digested by the Filter Aided Sample Preparation (FASP) procedure, followed by strong anion exchange (SAX) peptide fractionation. Peptides were analyzed by online LC-MS/MS using an LTQ-Orbitrap mass spectrometer. The relative abundance of the newly synthesized ( A l ) or preexisting peptides ( A m ) was defined as the ratio of mass spectrometric peak intensities of the unlabeled peptides ( I l ) or Arg6/Lys4-labeled peptides ( I m ) to the intensities of the Arg10/Lys8-labeled peptides ( I h ), respectively.

    Journal: International Journal of Proteomics

    Article Title: An Internal Standard-Assisted Synthesis and Degradation Proteomic Approach Reveals the Potential Linkage between VPS4B Depletion and Activation of Fatty Acid β-Oxidation in Breast Cancer Cells

    doi: 10.1155/2013/291415

    Figure Lengend Snippet: Using internal standard-assisted synthesis and degradation mass spectrometry (iSDMS) to study the roles of EGF on global protein synthesis and degradation. SKBR3_shVPS4B and the parental SKBR3 cells were first labeled with 13 C 6 -arginine (Arg6) and D 4 -lysine (Lys4) medium (labeled in red). After overnight serum starvation, Arg6/Lys4-labeled cells were stimulated with 100 ng/mL EGF in medium supplemented with regular arginine (Arg0) and lysine (Lys0) for 0, 2, 6, 12, and 24 hr (labeled in blue). Protein isolated from SKBR3_shVPS4B cells labeled with 13 C 6 15 N 4 -arginine (Arg10) and 13 C 6 15 N 2 -lysine (Lys8)—internal standard (labeled in green)—was spiked into each sample at a ratio of 1 : 3 (wt/wt). The mixtures were digested by the Filter Aided Sample Preparation (FASP) procedure, followed by strong anion exchange (SAX) peptide fractionation. Peptides were analyzed by online LC-MS/MS using an LTQ-Orbitrap mass spectrometer. The relative abundance of the newly synthesized ( A l ) or preexisting peptides ( A m ) was defined as the ratio of mass spectrometric peak intensities of the unlabeled peptides ( I l ) or Arg6/Lys4-labeled peptides ( I m ) to the intensities of the Arg10/Lys8-labeled peptides ( I h ), respectively.

    Article Snippet: LC-MS/MS Analysis Peptides were dissolved in 0.5% acetic acid (solvent A) and separated on a 10 cm reverse-phase PicoFrit spray tip (New Objective, Woburn, MA) packed in house with sub-2 μ m C18 resin (Prospereon Life Science, IL), using a nanoflow Xtreme simple liquid chromatography system (Microtech/CVC) coupled to a hybrid linear ion trap-Orbitrap mass spectrometer (LTQ Orbitrap, Thermo Scientific).

    Techniques: Mass Spectrometry, Labeling, Isolation, Sample Prep, Peptide Fractionation, Liquid Chromatography with Mass Spectroscopy, Synthesized