ltq orbitrapvelos mass spectrometer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ltq orbitrapvelos mass spectrometer
    The workflow illustrating the steps involved in proteomic analysis of midgut of female An. stephensi. Proteins were extracted from the midgut tissues and then subjected to SDS-PAGE, OFFGEL and bRPLC fractionation. Fractions collected were analyzed on analyzed on <t>LTQ-OrbitrapVelos</t> and LTQ-Orbitrap Elite mass spectrometer. Mascot and SEQUEST algorithms were used to perform database searches.
    Ltq Orbitrapvelos Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltq orbitrapvelos mass spectrometer/product/Thermo Fisher
    Average 89 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    ltq orbitrapvelos mass spectrometer - by Bioz Stars, 2020-05
    89/100 stars

    Images

    1) Product Images from "Mapping Anopheles stephensi midgut proteome using high-resolution mass spectrometry"

    Article Title: Mapping Anopheles stephensi midgut proteome using high-resolution mass spectrometry

    Journal: Data in Brief

    doi: 10.1016/j.dib.2018.02.028

    The workflow illustrating the steps involved in proteomic analysis of midgut of female An. stephensi. Proteins were extracted from the midgut tissues and then subjected to SDS-PAGE, OFFGEL and bRPLC fractionation. Fractions collected were analyzed on analyzed on LTQ-OrbitrapVelos and LTQ-Orbitrap Elite mass spectrometer. Mascot and SEQUEST algorithms were used to perform database searches.
    Figure Legend Snippet: The workflow illustrating the steps involved in proteomic analysis of midgut of female An. stephensi. Proteins were extracted from the midgut tissues and then subjected to SDS-PAGE, OFFGEL and bRPLC fractionation. Fractions collected were analyzed on analyzed on LTQ-OrbitrapVelos and LTQ-Orbitrap Elite mass spectrometer. Mascot and SEQUEST algorithms were used to perform database searches.

    Techniques Used: SDS Page, Fractionation, Mass Spectrometry

    2) Product Images from "Mapping Anopheles stephensi midgut proteome using high-resolution mass spectrometry"

    Article Title: Mapping Anopheles stephensi midgut proteome using high-resolution mass spectrometry

    Journal: Data in Brief

    doi: 10.1016/j.dib.2018.02.028

    The workflow illustrating the steps involved in proteomic analysis of midgut of female An. stephensi. Proteins were extracted from the midgut tissues and then subjected to SDS-PAGE, OFFGEL and bRPLC fractionation. Fractions collected were analyzed on analyzed on LTQ-OrbitrapVelos and LTQ-Orbitrap Elite mass spectrometer. Mascot and SEQUEST algorithms were used to perform database searches.
    Figure Legend Snippet: The workflow illustrating the steps involved in proteomic analysis of midgut of female An. stephensi. Proteins were extracted from the midgut tissues and then subjected to SDS-PAGE, OFFGEL and bRPLC fractionation. Fractions collected were analyzed on analyzed on LTQ-OrbitrapVelos and LTQ-Orbitrap Elite mass spectrometer. Mascot and SEQUEST algorithms were used to perform database searches.

    Techniques Used: SDS Page, Fractionation, Mass Spectrometry

    Related Articles

    High Performance Liquid Chromatography:

    Article Title: The extracellular proteome of two Bifidobacterium species reveals different adaptation strategies to low iron conditions
    Article Snippet: .. Acquisition of proteome data All data were acquired on an LTQ OrbitrapVelos mass spectrometer connected with an Easy-nLC 1000 HPLC system (Thermo Fisher Scientific, Germany). .. Peptide samples (4 μL) were loaded onto a frit column (75 μm inner diameter) packed with reverse phase C18 material AQ with 3 μm particle size and 200 Å pore size (Bischoff GmbH, Leonberg, Germany), and eluted with a flow rate of 300 nL per min. Solvent composition of buffer A was 0.1% formic acid in water, and buffer B contained 0.1% formic acid in acetonitrile.

    Flow Cytometry:

    Article Title: Mapping Anopheles stephensi midgut proteome using high-resolution mass spectrometry
    Article Snippet: .. In this study, a total of 58 LC–MS/MS runs, of which, 24 bRPLC fractions were performed on LTQ-Orbitrap Elite (Thermo Scientific, USA) mass spectrometer interfaced with Easy- nano LC II nano flow liquid chromatography system (Thermo Scientific), while the remaining 34 fractions (including in-gel and off-gel fractions) were analyzed on LTQ-OrbitrapVelos mass spectrometer interfaced with Proxeon Easy nLC system (Thermo Scientific, Bremen, Germany). .. The peptides from each fraction were reconstituted in 0.1% formic-acid and loaded on pre-column (75 µ × 2 cm) packed with magic C18 AQ (MichromBio-resources, USA) 5 µ particle and 100 Å pore size at flow rate of 5 µl per minute.

    Article Title: Mapping Anopheles stephensi midgut proteome using high-resolution mass spectrometry
    Article Snippet: .. 2.4 Mass spectrometry analysis In this study, a total of 58 LC–MS/MS runs, of which, 24 bRPLC fractions were performed on LTQ-Orbitrap Elite (Thermo Scientific, USA) mass spectrometer interfaced with Easy- nano LC II nano flow liquid chromatography system (Thermo Scientific), while the remaining 34 fractions (including in-gel and off-gel fractions) were analyzed on LTQ-OrbitrapVelos mass spectrometer interfaced with Proxeon Easy nLC system (Thermo Scientific, Bremen, Germany). .. The peptides from each fraction were reconstituted in 0.1% formic-acid and loaded on pre-column (75 µ × 2 cm) packed with magic C18 AQ (MichromBio-resources, USA) 5 µ particle and 100 Å pore size at flow rate of 5 µl per minute.

    Chromatography:

    Article Title: Killing persister cells and eradicating a biofilm infection by activating the ClpP protease
    Article Snippet: .. The peptide mix was then fractionated into 96 fractions by high pH reverse phase chromatography and concatenated into 24 fractions as previously described , and submitted to liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis in a LTQ OrbitrapVelos mass spectrometer (Thermo Fisher Scientific). .. Peptides were loaded into capillary columns (75 µm × 65 cm, Polymicro) packed with C18 beads (3 µm particles, Phenomenex) connected to a custom-made 4-column LC system .

    Generated:

    Article Title: Broad spectrum proteomics analysis of the inferior colliculus following acute hydrogen sulfide exposure
    Article Snippet: .. Following elution from the column, ions were generated using 2.6 kV on a taper tip in a New Objective Nanosource and entered into an LTQ-OrbitrapVelos mass spectrometer (Thermo Fisher, San Jose, CA). .. A full scan was taken in the LTQ, followed by data-dependent MS/MS analysis of the top 6 peaks.

    Mass Spectrometry:

    Article Title: Mapping Anopheles stephensi midgut proteome using high-resolution mass spectrometry
    Article Snippet: .. In this study, a total of 58 LC–MS/MS runs, of which, 24 bRPLC fractions were performed on LTQ-Orbitrap Elite (Thermo Scientific, USA) mass spectrometer interfaced with Easy- nano LC II nano flow liquid chromatography system (Thermo Scientific), while the remaining 34 fractions (including in-gel and off-gel fractions) were analyzed on LTQ-OrbitrapVelos mass spectrometer interfaced with Proxeon Easy nLC system (Thermo Scientific, Bremen, Germany). .. The peptides from each fraction were reconstituted in 0.1% formic-acid and loaded on pre-column (75 µ × 2 cm) packed with magic C18 AQ (MichromBio-resources, USA) 5 µ particle and 100 Å pore size at flow rate of 5 µl per minute.

    Article Title: The extracellular proteome of two Bifidobacterium species reveals different adaptation strategies to low iron conditions
    Article Snippet: .. Acquisition of proteome data All data were acquired on an LTQ OrbitrapVelos mass spectrometer connected with an Easy-nLC 1000 HPLC system (Thermo Fisher Scientific, Germany). .. Peptide samples (4 μL) were loaded onto a frit column (75 μm inner diameter) packed with reverse phase C18 material AQ with 3 μm particle size and 200 Å pore size (Bischoff GmbH, Leonberg, Germany), and eluted with a flow rate of 300 nL per min. Solvent composition of buffer A was 0.1% formic acid in water, and buffer B contained 0.1% formic acid in acetonitrile.

    Article Title: Variable Characteristics of Bacteriocin-Producing Streptococcus salivarius Strains Isolated from Malaysian Subjects
    Article Snippet: .. The system was coupled to an LTQ OrbitrapVelos mass spectrometer equipped with a Nanospray II source (Thermo Fisher Scientific). ..

    Article Title: Broad spectrum proteomics analysis of the inferior colliculus following acute hydrogen sulfide exposure
    Article Snippet: .. Following elution from the column, ions were generated using 2.6 kV on a taper tip in a New Objective Nanosource and entered into an LTQ-OrbitrapVelos mass spectrometer (Thermo Fisher, San Jose, CA). .. A full scan was taken in the LTQ, followed by data-dependent MS/MS analysis of the top 6 peaks.

    Article Title: Mapping Anopheles stephensi midgut proteome using high-resolution mass spectrometry
    Article Snippet: .. 2.4 Mass spectrometry analysis In this study, a total of 58 LC–MS/MS runs, of which, 24 bRPLC fractions were performed on LTQ-Orbitrap Elite (Thermo Scientific, USA) mass spectrometer interfaced with Easy- nano LC II nano flow liquid chromatography system (Thermo Scientific), while the remaining 34 fractions (including in-gel and off-gel fractions) were analyzed on LTQ-OrbitrapVelos mass spectrometer interfaced with Proxeon Easy nLC system (Thermo Scientific, Bremen, Germany). .. The peptides from each fraction were reconstituted in 0.1% formic-acid and loaded on pre-column (75 µ × 2 cm) packed with magic C18 AQ (MichromBio-resources, USA) 5 µ particle and 100 Å pore size at flow rate of 5 µl per minute.

    Article Title: Killing persister cells and eradicating a biofilm infection by activating the ClpP protease
    Article Snippet: .. The peptide mix was then fractionated into 96 fractions by high pH reverse phase chromatography and concatenated into 24 fractions as previously described , and submitted to liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis in a LTQ OrbitrapVelos mass spectrometer (Thermo Fisher Scientific). .. Peptides were loaded into capillary columns (75 µm × 65 cm, Polymicro) packed with C18 beads (3 µm particles, Phenomenex) connected to a custom-made 4-column LC system .

    Article Title:
    Article Snippet: .. LC-tandem mass spectrometry (MS/MS) analysis was carried out on an LTQ OrbitrapVelos mass spectrometer (Thermo Scientific) equipped with a Thermo nanospray source (Thermo, San Jose, CA) and coupled with an Eksigent NanoLC Ultra 2D system (AB SCIEX). .. Separation of peptide mixtures was achieved on a 75-μm-inner diameter microcapillary trap column packed with 18 cm of Magic C18 resin (3 μm, 200 Å; Michrom Bioresources) with a gradient of acetonitrile (3–35%) in 0.1% formic acid for 145 min at a flow rate of 300 nl/min.

    Article Title: Transcriptomic and proteomic analysis of pre-diapause and non-diapause eggs of migratory locust, Locusta migratoria L. (Orthoptera: Acridoidea)
    Article Snippet: .. Nano LC-MS/MS analysis of each of these fractions was performed using a LTQ-OrbitrapVelos mass spectrometer (Thermo Fisher Scientific Inc. Rockford, IL, USA) equipped with nano electrospray ionization . .. Peptides were identified by searching against a specified database containing 41,407 mRNA sequences using a MS/MS data interpretation algorithm within Mascot.

    Tandem Mass Spectroscopy:

    Article Title:
    Article Snippet: .. LC-tandem mass spectrometry (MS/MS) analysis was carried out on an LTQ OrbitrapVelos mass spectrometer (Thermo Scientific) equipped with a Thermo nanospray source (Thermo, San Jose, CA) and coupled with an Eksigent NanoLC Ultra 2D system (AB SCIEX). .. Separation of peptide mixtures was achieved on a 75-μm-inner diameter microcapillary trap column packed with 18 cm of Magic C18 resin (3 μm, 200 Å; Michrom Bioresources) with a gradient of acetonitrile (3–35%) in 0.1% formic acid for 145 min at a flow rate of 300 nl/min.

    Reversed-phase Chromatography:

    Article Title: Killing persister cells and eradicating a biofilm infection by activating the ClpP protease
    Article Snippet: .. The peptide mix was then fractionated into 96 fractions by high pH reverse phase chromatography and concatenated into 24 fractions as previously described , and submitted to liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis in a LTQ OrbitrapVelos mass spectrometer (Thermo Fisher Scientific). .. Peptides were loaded into capillary columns (75 µm × 65 cm, Polymicro) packed with C18 beads (3 µm particles, Phenomenex) connected to a custom-made 4-column LC system .

    Liquid Chromatography:

    Article Title: Mapping Anopheles stephensi midgut proteome using high-resolution mass spectrometry
    Article Snippet: .. In this study, a total of 58 LC–MS/MS runs, of which, 24 bRPLC fractions were performed on LTQ-Orbitrap Elite (Thermo Scientific, USA) mass spectrometer interfaced with Easy- nano LC II nano flow liquid chromatography system (Thermo Scientific), while the remaining 34 fractions (including in-gel and off-gel fractions) were analyzed on LTQ-OrbitrapVelos mass spectrometer interfaced with Proxeon Easy nLC system (Thermo Scientific, Bremen, Germany). .. The peptides from each fraction were reconstituted in 0.1% formic-acid and loaded on pre-column (75 µ × 2 cm) packed with magic C18 AQ (MichromBio-resources, USA) 5 µ particle and 100 Å pore size at flow rate of 5 µl per minute.

    Article Title: Mapping Anopheles stephensi midgut proteome using high-resolution mass spectrometry
    Article Snippet: .. 2.4 Mass spectrometry analysis In this study, a total of 58 LC–MS/MS runs, of which, 24 bRPLC fractions were performed on LTQ-Orbitrap Elite (Thermo Scientific, USA) mass spectrometer interfaced with Easy- nano LC II nano flow liquid chromatography system (Thermo Scientific), while the remaining 34 fractions (including in-gel and off-gel fractions) were analyzed on LTQ-OrbitrapVelos mass spectrometer interfaced with Proxeon Easy nLC system (Thermo Scientific, Bremen, Germany). .. The peptides from each fraction were reconstituted in 0.1% formic-acid and loaded on pre-column (75 µ × 2 cm) packed with magic C18 AQ (MichromBio-resources, USA) 5 µ particle and 100 Å pore size at flow rate of 5 µl per minute.

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  • News
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  • 89
    Thermo Fisher ltq orbitrapvelos mass spectrometer
    The workflow illustrating the steps involved in proteomic analysis of midgut of female An. stephensi. Proteins were extracted from the midgut tissues and then subjected to SDS-PAGE, OFFGEL and bRPLC fractionation. Fractions collected were analyzed on analyzed on <t>LTQ-OrbitrapVelos</t> and LTQ-Orbitrap Elite mass spectrometer. Mascot and SEQUEST algorithms were used to perform database searches.
    Ltq Orbitrapvelos Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltq orbitrapvelos mass spectrometer/product/Thermo Fisher
    Average 89 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    ltq orbitrapvelos mass spectrometer - by Bioz Stars, 2020-05
    89/100 stars
      Buy from Supplier

    88
    Thermo Fisher ltq orbitrapvelos etd
    The workflow illustrating the steps involved in proteomic analysis of midgut of female An. stephensi. Proteins were extracted from the midgut tissues and then subjected to SDS-PAGE, OFFGEL and bRPLC fractionation. Fractions collected were analyzed on analyzed on <t>LTQ-OrbitrapVelos</t> and LTQ-Orbitrap Elite mass spectrometer. Mascot and SEQUEST algorithms were used to perform database searches.
    Ltq Orbitrapvelos Etd, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltq orbitrapvelos etd/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ltq orbitrapvelos etd - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

    89
    Thermo Fisher ltq orbitrapvelos
    The workflow illustrating the steps involved in proteomic analysis of midgut of female An. stephensi. Proteins were extracted from the midgut tissues and then subjected to SDS-PAGE, OFFGEL and bRPLC fractionation. Fractions collected were analyzed on analyzed on <t>LTQ-OrbitrapVelos</t> and LTQ-Orbitrap Elite mass spectrometer. Mascot and SEQUEST algorithms were used to perform database searches.
    Ltq Orbitrapvelos, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltq orbitrapvelos/product/Thermo Fisher
    Average 89 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    ltq orbitrapvelos - by Bioz Stars, 2020-05
    89/100 stars
      Buy from Supplier

    Image Search Results


    The workflow illustrating the steps involved in proteomic analysis of midgut of female An. stephensi. Proteins were extracted from the midgut tissues and then subjected to SDS-PAGE, OFFGEL and bRPLC fractionation. Fractions collected were analyzed on analyzed on LTQ-OrbitrapVelos and LTQ-Orbitrap Elite mass spectrometer. Mascot and SEQUEST algorithms were used to perform database searches.

    Journal: Data in Brief

    Article Title: Mapping Anopheles stephensi midgut proteome using high-resolution mass spectrometry

    doi: 10.1016/j.dib.2018.02.028

    Figure Lengend Snippet: The workflow illustrating the steps involved in proteomic analysis of midgut of female An. stephensi. Proteins were extracted from the midgut tissues and then subjected to SDS-PAGE, OFFGEL and bRPLC fractionation. Fractions collected were analyzed on analyzed on LTQ-OrbitrapVelos and LTQ-Orbitrap Elite mass spectrometer. Mascot and SEQUEST algorithms were used to perform database searches.

    Article Snippet: 2.4 Mass spectrometry analysis In this study, a total of 58 LC–MS/MS runs, of which, 24 bRPLC fractions were performed on LTQ-Orbitrap Elite (Thermo Scientific, USA) mass spectrometer interfaced with Easy- nano LC II nano flow liquid chromatography system (Thermo Scientific), while the remaining 34 fractions (including in-gel and off-gel fractions) were analyzed on LTQ-OrbitrapVelos mass spectrometer interfaced with Proxeon Easy nLC system (Thermo Scientific, Bremen, Germany).

    Techniques: SDS Page, Fractionation, Mass Spectrometry

    The workflow illustrating the steps involved in proteomic analysis of midgut of female An. stephensi. Proteins were extracted from the midgut tissues and then subjected to SDS-PAGE, OFFGEL and bRPLC fractionation. Fractions collected were analyzed on analyzed on LTQ-OrbitrapVelos and LTQ-Orbitrap Elite mass spectrometer. Mascot and SEQUEST algorithms were used to perform database searches.

    Journal: Data in Brief

    Article Title: Mapping Anopheles stephensi midgut proteome using high-resolution mass spectrometry

    doi: 10.1016/j.dib.2018.02.028

    Figure Lengend Snippet: The workflow illustrating the steps involved in proteomic analysis of midgut of female An. stephensi. Proteins were extracted from the midgut tissues and then subjected to SDS-PAGE, OFFGEL and bRPLC fractionation. Fractions collected were analyzed on analyzed on LTQ-OrbitrapVelos and LTQ-Orbitrap Elite mass spectrometer. Mascot and SEQUEST algorithms were used to perform database searches.

    Article Snippet: In this study, a total of 58 LC–MS/MS runs, of which, 24 bRPLC fractions were performed on LTQ-Orbitrap Elite (Thermo Scientific, USA) mass spectrometer interfaced with Easy- nano LC II nano flow liquid chromatography system (Thermo Scientific), while the remaining 34 fractions (including in-gel and off-gel fractions) were analyzed on LTQ-OrbitrapVelos mass spectrometer interfaced with Proxeon Easy nLC system (Thermo Scientific, Bremen, Germany).

    Techniques: SDS Page, Fractionation, Mass Spectrometry