ltq orbitrap lc ms  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ltq orbitrap lc ms
    Ltq Orbitrap Lc Ms, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltq orbitrap lc ms/product/Thermo Fisher
    Average 88 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    ltq orbitrap lc ms - by Bioz Stars, 2020-07
    88/100 stars

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    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Study on the Polar Extracts of Dendrobium nobile, D. officinale, D. loddigesii, and Flickingeria fimbriata: Metabolite Identification, Content Evaluation, and Bioactivity Assay
    Article Snippet: .. The ESI (electrospray ionization) mass spectra and HRESIMS were obtained using an LTQ-Orbitrap LC-MS (Thermo Fisher, Frankfurt, German). ..

    Article Title: New Dimeric Members of the Phomoxanthone Family: Phomolactonexanthones A, B and Deacetylphomoxanthone C Isolated from the Fungus Phomopsis sp.
    Article Snippet: .. HRMS data were measured on LTQ Orbitrap LC-MS (Thermo, Rockford, IL, USA). .. Column chromatography was performed using silica gel (200–300 mesh, Qingdao Marine Chemcials, Qingdao, China).

    Article Title: Six New Polyketide Decalin Compounds from Mangrove Endophytic Fungus Penicillium aurantiogriseum 328#
    Article Snippet: .. The EI mass spectra and high-resolution mass spectra were obtained using MAT95XP (ThermoFinnigan, Bremen, Germany) high resolution mass spectrometer and a LTQ-Orbitrap LC-MS (Thermo Fisher, Frankfurt, German). .. HPLC was performed using a 515 pump with a UV 2487 detector (Waters, Milford, MA, USA) and an Ultimate XB-C-18 column (250 mm × 10 mm, 5 μm; Welch, MD, USA).

    Article Title: Phomopsichin A–D; Four New Chromone Derivatives from Mangrove Endophytic Fungus Phomopsis sp. 33#
    Article Snippet: .. The HRESIMS were obtained using a LTQ-Orbitrap LC-MS (Thermo Fisher, Frankfurt, Germany). .. Single-crystal data were carried out on an Agilent Technologies Gemini A Ultra system (Agilent Tech, Santa Clara, CA, USA).

    Article Title: Mechanistic studies of DepR in regulating FK228 biosynthesis in Chromobacterium violaceum no. 968
    Article Snippet: .. The residue was resuspended in 350 μL methanol and 15 μL of such organic extract was analyzed with a Thermo Scientific LTQ Orbitrap LC-MS for the detection and quantification of FK228 production. .. The depR gene was excised from pBMTL-3- depR and cloned into pET28a for overexpression in E . coli BL21(DE3).

    Article Title: Loddigesiinols G–J: α-Glucosidase Inhibitors from Dendrobium loddigesii
    Article Snippet: .. High-resolution mass spectra were measured on a LTQ-Orbitrap LC-MS (Thermo Fisher, Frankfurt, German). .. HPLC was performed using a 515 pump with a UV 2487 detector (Waters, Milford, USA) and an Ultimate XB-C-18 column (250 × 10 mm, 5 μL; Welch, MD, USA).

    Mass Spectrometry:

    Article Title: Six New Polyketide Decalin Compounds from Mangrove Endophytic Fungus Penicillium aurantiogriseum 328#
    Article Snippet: .. The EI mass spectra and high-resolution mass spectra were obtained using MAT95XP (ThermoFinnigan, Bremen, Germany) high resolution mass spectrometer and a LTQ-Orbitrap LC-MS (Thermo Fisher, Frankfurt, German). .. HPLC was performed using a 515 pump with a UV 2487 detector (Waters, Milford, MA, USA) and an Ultimate XB-C-18 column (250 mm × 10 mm, 5 μm; Welch, MD, USA).

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    Thermo Fisher ltq orbitrap mass spectrometer
    Human Parkin Ser 65 is a substrate of human PINK1 upon CCCP stimulation. ( a ) Confirmation by mass spectrometry that Ser 65 of human Parkin is phosphorylated by CCCP-induced activation of human wild-type PINK1-FLAG. Flp-In T-Rex HEK293 cells expressing FLAG-empty, wild-type PINK1-FLAG, and kinase-inactive PINK1-FLAG (D384A) were co-transfected with HA-Parkin, induced with doxycycline and stimulated with 10 μM of CCCP for 3 h. Whole-cell extracts were obtained following lysis with 1% Triton and approximately 30 mg of whole-cell extract were subjected to immunoprecipitation with anti-HA-agarose and run on 10% SDS-PAGE and stained with colloidal Coomassie blue. Coomassie-stained bands migrating with the expected molecular mass of HA-Parkin were excised from the gel, digested with trypsin, and subjected to high performance liquid chromatography with tandem mass spectrometry (LC-MS-MS) on an <t>LTQ-Orbitrap</t> mass spectrometer. Extracted ion chromatogram analysis of Ser 131 and Ser 65 phosphopeptide (3 + R.NDWTVQNCDLDQQ S IVHIVQRPWR.K+P). The total signal intensity of the phosphopeptide is plotted on the y -axis and retention time is plotted on the x -axis. The m / z value corresponding to the Ser 131 phosphopeptide was detected in all conditions whilst that of the Ser 65 phosphopeptide was only detected in samples from wild-type PINK1-FLAG-expressing cells following CCCP treatment. ( b ) Characterization of Parkin phospho-Ser 65 antibody. Flp-In T-Rex HEK293 cells expressing FLAG-empty, wild-type PINK1-FLAG, and kinase-inactive PINK1-FLAG were co-transfected with untagged wild-type (WT) or Ser 65 Ala (S65A) mutant Parkin, induced with doxycycline and stimulated with 10 μM of CCCP for 3 h. 0.25 mg of 1% Triton whole-cell lysate were subjected to immunoprecipitation with anti-Parkin antibody (S966C) covalently coupled to protein G Sepharose and then immunoblotted with anti-phospho-Ser 65 antibody in the presence of dephosphorylated peptide. Ten per cent of the immunoprecipitate (IP) was immunoblotted with total anti-Parkin antibody. Twenty five micrograms of whole cell lysate was immunoblotted with total PINK1 antibody.
    Ltq Orbitrap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1665 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltq orbitrap mass spectrometer/product/Thermo Fisher
    Average 95 stars, based on 1665 article reviews
    Price from $9.99 to $1999.99
    ltq orbitrap mass spectrometer - by Bioz Stars, 2020-07
    95/100 stars
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    94
    Thermo Fisher ltq orbitrap xl
    Analysis of MASL protein subunits. ( a ) MASL from Sigma (lane 1) or Sentrimed (lane 2) was analyzed by reducing and nonreducing SDS-PAGE. ( b ) Coomassie stained bands were excised, extracted and digested with trypsin, reduced and alkylated with iodoacetamide, and sequenced using data dependent acquisition by LC-MS/MS on a Thermo Scientific <t>LTQ</t> <t>Orbitrap</t> XL. All peptides were identical in primary sequence and contained a single cysteine at residue 243 (bold in figure).
    Ltq Orbitrap Xl, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 567 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltq orbitrap xl/product/Thermo Fisher
    Average 94 stars, based on 567 article reviews
    Price from $9.99 to $1999.99
    ltq orbitrap xl - by Bioz Stars, 2020-07
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    89
    Thermo Fisher ltq orbitrap classic mass spectrometer
    SILAC workflow. A combined strategy involving RNAi against Cdc7, SDS-PAGE protein fractionation, LC–MS/MS data acquisition with a <t>LTQ-Orbitrap</t> (Thermo Fisher Scientific), and downstream bioinformatics for identification, quantification, and functional annotation was applied in this study.
    Ltq Orbitrap Classic Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltq orbitrap classic mass spectrometer/product/Thermo Fisher
    Average 89 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    ltq orbitrap classic mass spectrometer - by Bioz Stars, 2020-07
    89/100 stars
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    92
    Thermo Fisher ltq orbitrap velos pro mass spectrometer
    Identification of linoleic acid in the free fatty acid fraction purified from canine stratum corneum by APCI LC-MS. RSLC Dionex-U3000 coupled to <t>LTQ-Orbitrap</t> <t>Velos</t> Pro, Thermofisher Scientific with an APCI source. a Free fatty acids spectra. b Linoleic acid spectra- standard use
    Ltq Orbitrap Velos Pro Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 205 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltq orbitrap velos pro mass spectrometer/product/Thermo Fisher
    Average 92 stars, based on 205 article reviews
    Price from $9.99 to $1999.99
    ltq orbitrap velos pro mass spectrometer - by Bioz Stars, 2020-07
    92/100 stars
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    Human Parkin Ser 65 is a substrate of human PINK1 upon CCCP stimulation. ( a ) Confirmation by mass spectrometry that Ser 65 of human Parkin is phosphorylated by CCCP-induced activation of human wild-type PINK1-FLAG. Flp-In T-Rex HEK293 cells expressing FLAG-empty, wild-type PINK1-FLAG, and kinase-inactive PINK1-FLAG (D384A) were co-transfected with HA-Parkin, induced with doxycycline and stimulated with 10 μM of CCCP for 3 h. Whole-cell extracts were obtained following lysis with 1% Triton and approximately 30 mg of whole-cell extract were subjected to immunoprecipitation with anti-HA-agarose and run on 10% SDS-PAGE and stained with colloidal Coomassie blue. Coomassie-stained bands migrating with the expected molecular mass of HA-Parkin were excised from the gel, digested with trypsin, and subjected to high performance liquid chromatography with tandem mass spectrometry (LC-MS-MS) on an LTQ-Orbitrap mass spectrometer. Extracted ion chromatogram analysis of Ser 131 and Ser 65 phosphopeptide (3 + R.NDWTVQNCDLDQQ S IVHIVQRPWR.K+P). The total signal intensity of the phosphopeptide is plotted on the y -axis and retention time is plotted on the x -axis. The m / z value corresponding to the Ser 131 phosphopeptide was detected in all conditions whilst that of the Ser 65 phosphopeptide was only detected in samples from wild-type PINK1-FLAG-expressing cells following CCCP treatment. ( b ) Characterization of Parkin phospho-Ser 65 antibody. Flp-In T-Rex HEK293 cells expressing FLAG-empty, wild-type PINK1-FLAG, and kinase-inactive PINK1-FLAG were co-transfected with untagged wild-type (WT) or Ser 65 Ala (S65A) mutant Parkin, induced with doxycycline and stimulated with 10 μM of CCCP for 3 h. 0.25 mg of 1% Triton whole-cell lysate were subjected to immunoprecipitation with anti-Parkin antibody (S966C) covalently coupled to protein G Sepharose and then immunoblotted with anti-phospho-Ser 65 antibody in the presence of dephosphorylated peptide. Ten per cent of the immunoprecipitate (IP) was immunoblotted with total anti-Parkin antibody. Twenty five micrograms of whole cell lysate was immunoblotted with total PINK1 antibody.

    Journal: Open Biology

    Article Title: PINK1 is activated by mitochondrial membrane potential depolarization and stimulates Parkin E3 ligase activity by phosphorylating Serine 65

    doi: 10.1098/rsob.120080

    Figure Lengend Snippet: Human Parkin Ser 65 is a substrate of human PINK1 upon CCCP stimulation. ( a ) Confirmation by mass spectrometry that Ser 65 of human Parkin is phosphorylated by CCCP-induced activation of human wild-type PINK1-FLAG. Flp-In T-Rex HEK293 cells expressing FLAG-empty, wild-type PINK1-FLAG, and kinase-inactive PINK1-FLAG (D384A) were co-transfected with HA-Parkin, induced with doxycycline and stimulated with 10 μM of CCCP for 3 h. Whole-cell extracts were obtained following lysis with 1% Triton and approximately 30 mg of whole-cell extract were subjected to immunoprecipitation with anti-HA-agarose and run on 10% SDS-PAGE and stained with colloidal Coomassie blue. Coomassie-stained bands migrating with the expected molecular mass of HA-Parkin were excised from the gel, digested with trypsin, and subjected to high performance liquid chromatography with tandem mass spectrometry (LC-MS-MS) on an LTQ-Orbitrap mass spectrometer. Extracted ion chromatogram analysis of Ser 131 and Ser 65 phosphopeptide (3 + R.NDWTVQNCDLDQQ S IVHIVQRPWR.K+P). The total signal intensity of the phosphopeptide is plotted on the y -axis and retention time is plotted on the x -axis. The m / z value corresponding to the Ser 131 phosphopeptide was detected in all conditions whilst that of the Ser 65 phosphopeptide was only detected in samples from wild-type PINK1-FLAG-expressing cells following CCCP treatment. ( b ) Characterization of Parkin phospho-Ser 65 antibody. Flp-In T-Rex HEK293 cells expressing FLAG-empty, wild-type PINK1-FLAG, and kinase-inactive PINK1-FLAG were co-transfected with untagged wild-type (WT) or Ser 65 Ala (S65A) mutant Parkin, induced with doxycycline and stimulated with 10 μM of CCCP for 3 h. 0.25 mg of 1% Triton whole-cell lysate were subjected to immunoprecipitation with anti-Parkin antibody (S966C) covalently coupled to protein G Sepharose and then immunoblotted with anti-phospho-Ser 65 antibody in the presence of dephosphorylated peptide. Ten per cent of the immunoprecipitate (IP) was immunoblotted with total anti-Parkin antibody. Twenty five micrograms of whole cell lysate was immunoblotted with total PINK1 antibody.

    Article Snippet: Isolated phosphopeptides were analysed by LC-MS-MS on a proxeon Easy-nLC nano liquid chromatography system coupled to a Thermo LTQ-orbitrap mass spectrometer.

    Techniques: Mass Spectrometry, Activation Assay, Expressing, Transfection, Lysis, Immunoprecipitation, SDS Page, Staining, High Performance Liquid Chromatography, Liquid Chromatography with Mass Spectroscopy, Mutagenesis

    Analysis of MASL protein subunits. ( a ) MASL from Sigma (lane 1) or Sentrimed (lane 2) was analyzed by reducing and nonreducing SDS-PAGE. ( b ) Coomassie stained bands were excised, extracted and digested with trypsin, reduced and alkylated with iodoacetamide, and sequenced using data dependent acquisition by LC-MS/MS on a Thermo Scientific LTQ Orbitrap XL. All peptides were identical in primary sequence and contained a single cysteine at residue 243 (bold in figure).

    Journal: PLoS ONE

    Article Title: Plant Lectin Can Target Receptors Containing Sialic Acid, Exemplified by Podoplanin, to Inhibit Transformed Cell Growth and Migration

    doi: 10.1371/journal.pone.0041845

    Figure Lengend Snippet: Analysis of MASL protein subunits. ( a ) MASL from Sigma (lane 1) or Sentrimed (lane 2) was analyzed by reducing and nonreducing SDS-PAGE. ( b ) Coomassie stained bands were excised, extracted and digested with trypsin, reduced and alkylated with iodoacetamide, and sequenced using data dependent acquisition by LC-MS/MS on a Thermo Scientific LTQ Orbitrap XL. All peptides were identical in primary sequence and contained a single cysteine at residue 243 (bold in figure).

    Article Snippet: Extracted protein was reduced and alkylated with iodoacetamide, and sequenced using data dependent acquisition by LC-MS/MS on a Thermo Scientific LTQ Orbitrap XL.

    Techniques: SDS Page, Staining, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Sequencing

    SILAC workflow. A combined strategy involving RNAi against Cdc7, SDS-PAGE protein fractionation, LC–MS/MS data acquisition with a LTQ-Orbitrap (Thermo Fisher Scientific), and downstream bioinformatics for identification, quantification, and functional annotation was applied in this study.

    Journal: Journal of proteome research

    Article Title: Quantitative Proteomics Reveals a “Poised Quiescence” Cellular State after Triggering the DNA Replication Origin Activation Checkpoint

    doi: 10.1021/pr100678k

    Figure Lengend Snippet: SILAC workflow. A combined strategy involving RNAi against Cdc7, SDS-PAGE protein fractionation, LC–MS/MS data acquisition with a LTQ-Orbitrap (Thermo Fisher Scientific), and downstream bioinformatics for identification, quantification, and functional annotation was applied in this study.

    Article Snippet: LC–MS/MS was performed with an LTQ-Orbitrap Classic mass spectrometer (Thermo Fisher Scientific, U.K.).

    Techniques: SDS Page, Fractionation, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Functional Assay

    Identification of linoleic acid in the free fatty acid fraction purified from canine stratum corneum by APCI LC-MS. RSLC Dionex-U3000 coupled to LTQ-Orbitrap Velos Pro, Thermofisher Scientific with an APCI source. a Free fatty acids spectra. b Linoleic acid spectra- standard use

    Journal: Archives of Dermatological Research

    Article Title: Linoleate-enriched diet increases both linoleic acid esterified to omega hydroxy very long chain fatty acids and free ceramides of canine stratum corneum without effect on protein-bound ceramides and skin barrier function

    doi: 10.1007/s00403-018-1845-5

    Figure Lengend Snippet: Identification of linoleic acid in the free fatty acid fraction purified from canine stratum corneum by APCI LC-MS. RSLC Dionex-U3000 coupled to LTQ-Orbitrap Velos Pro, Thermofisher Scientific with an APCI source. a Free fatty acids spectra. b Linoleic acid spectra- standard use

    Article Snippet: A 5 µl sample was injected in a RSLC Dionex-U300 column coupled to a LTQ-Orbitrap Velos Pro mass spectrometer (Thermofisher Scientific, Villebon-sur-Yvette, France) equipped with an atmospheric-pressure chemical ionization (APCI) interface.

    Techniques: Purification, Liquid Chromatography with Mass Spectroscopy