ltq orbitrap discovery mass spectrometer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ltq orbitrap discovery mass spectrometer
    Partial sequence (162–174) is present in unprocessed ABCA3 and missing in processed protein form. A : Intensity profiles of an m/z range corresponding to the monoisotopic peak of the doubly-charged ion at m/z 819.885 ( m/z window ±5 ppm) for 190 kDa and 170 kDa gel bands acquired in <t>Orbitrap.</t> B : Identification of the ABCA3 tryptic peptide comprising amino acid residues 162–174 of the precursor protein and containing methionine sulfoxide at position 164. MS/MS spectra after CID fragmentation measured in the <t>LTQ</t> ion trap are shown. Major b and y ions are labelled on the spectra and all fragment ions obtained are marked on the identified sequence. The ion at m/z 811 corresponds to the loss of water from the parent ion.
    Ltq Orbitrap Discovery Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltq orbitrap discovery mass spectrometer/product/Thermo Fisher
    Average 95 stars, based on 90 article reviews
    Price from $9.99 to $1999.99
    ltq orbitrap discovery mass spectrometer - by Bioz Stars, 2020-05
    95/100 stars

    Images

    1) Product Images from "Analysis of the Proteolytic Processing of ABCA3: Identification of Cleavage Site and Involved Proteases"

    Article Title: Analysis of the Proteolytic Processing of ABCA3: Identification of Cleavage Site and Involved Proteases

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0152594

    Partial sequence (162–174) is present in unprocessed ABCA3 and missing in processed protein form. A : Intensity profiles of an m/z range corresponding to the monoisotopic peak of the doubly-charged ion at m/z 819.885 ( m/z window ±5 ppm) for 190 kDa and 170 kDa gel bands acquired in Orbitrap. B : Identification of the ABCA3 tryptic peptide comprising amino acid residues 162–174 of the precursor protein and containing methionine sulfoxide at position 164. MS/MS spectra after CID fragmentation measured in the LTQ ion trap are shown. Major b and y ions are labelled on the spectra and all fragment ions obtained are marked on the identified sequence. The ion at m/z 811 corresponds to the loss of water from the parent ion.
    Figure Legend Snippet: Partial sequence (162–174) is present in unprocessed ABCA3 and missing in processed protein form. A : Intensity profiles of an m/z range corresponding to the monoisotopic peak of the doubly-charged ion at m/z 819.885 ( m/z window ±5 ppm) for 190 kDa and 170 kDa gel bands acquired in Orbitrap. B : Identification of the ABCA3 tryptic peptide comprising amino acid residues 162–174 of the precursor protein and containing methionine sulfoxide at position 164. MS/MS spectra after CID fragmentation measured in the LTQ ion trap are shown. Major b and y ions are labelled on the spectra and all fragment ions obtained are marked on the identified sequence. The ion at m/z 811 corresponds to the loss of water from the parent ion.

    Techniques Used: Sequencing, Mass Spectrometry

    2) Product Images from "Peptides Presented by HLA-DR Molecules in Synovia of Patients with Rheumatoid Arthritis or Antibiotic-Refractory Lyme Arthritis *"

    Article Title: Peptides Presented by HLA-DR Molecules in Synovia of Patients with Rheumatoid Arthritis or Antibiotic-Refractory Lyme Arthritis *

    Journal: Molecular & Cellular Proteomics : MCP

    doi: 10.1074/mcp.M110.002477

    LTQ-Orbitrap CID tandem mass spectra of two overlapping peptides derived from cathepsin S and post-translationally modified with disulfide-linked cysteine. A , KTGKLVSLSAQNLVDC-Cys [M + 3H] 3+ m/z 598.98, neutral mass 1793.90 Da. B , LKTGKLVSLSAQNLVDC-Cys [M + 3H] 3+ m/z 636.67, neutral mass 1906.98 Da. Because the difference between the two peptides is at the amino terminus, the two b-series of fragments exhibit a mass shift consistent with the presence of the additional amino-terminal Leu residue that corresponds to the difference between the two molecular masses, whereas the y-series show common fragments.
    Figure Legend Snippet: LTQ-Orbitrap CID tandem mass spectra of two overlapping peptides derived from cathepsin S and post-translationally modified with disulfide-linked cysteine. A , KTGKLVSLSAQNLVDC-Cys [M + 3H] 3+ m/z 598.98, neutral mass 1793.90 Da. B , LKTGKLVSLSAQNLVDC-Cys [M + 3H] 3+ m/z 636.67, neutral mass 1906.98 Da. Because the difference between the two peptides is at the amino terminus, the two b-series of fragments exhibit a mass shift consistent with the presence of the additional amino-terminal Leu residue that corresponds to the difference between the two molecular masses, whereas the y-series show common fragments.

    Techniques Used: Derivative Assay, Modification

    3) Product Images from "Estrogenic and cytotoxic potentials of compounds isolated from Millettia macrophylla Benth (Fabaceae): towards a better understanding of its underlying mechanisms"

    Article Title: Estrogenic and cytotoxic potentials of compounds isolated from Millettia macrophylla Benth (Fabaceae): towards a better understanding of its underlying mechanisms

    Journal: BMC Complementary and Alternative Medicine

    doi: 10.1186/s12906-016-1385-5

    Base peak chromatogram of Millettia macrophylla phenolic fraction recorded in UHPLC-ESI (+)-LTQ-Orbitrap
    Figure Legend Snippet: Base peak chromatogram of Millettia macrophylla phenolic fraction recorded in UHPLC-ESI (+)-LTQ-Orbitrap

    Techniques Used:

    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Chemical Proteomic Platform To Identify Citrullinated Proteins
    Article Snippet: .. LC-MS/MS analysis was performed on an LTQ Orbitrap Discovery mass spectrometer (ThermoFisher) coupled to an Agilent 1200 series HPLC. .. Digests were pressure loaded onto a 250 μm fused silica desalting column packed with 4 cm of Aqua C18 reverse-phase resin (Phenomenex).

    Article Title: Identification of deubiquitinase targets of isothiocyanates using SILAC-assisted quantitative mass spectrometry
    Article Snippet: .. LC/LC-MS/MS analysis was performed on an LTQ Orbitrap Discovery mass spectrometer (ThermoFisher) coupled to an Agilent 1200 series HPLC. .. Digests were pressure loaded onto a 250 μm fused silica desalting column packed with 4 cm of Aqua C18 reverse phase resin (Phenomenex).

    Flow Cytometry:

    Article Title: Ultrasound-promoted synthesis of 2-organoselanyl-naphthalenes using Oxone® in aqueous medium as an oxidizing agent
    Article Snippet: .. A Shimadzu GC-MS-QP2010 was used to obtain the low-resolution mass spectra (MS), while a LTQ Orbitrap Discovery mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) was employed to obtain the high-resolution mass spectra (HRMS), the experiments were performed via direct infusion of sample (flow: 10 μL/min) in the positive-ion mode using electrospray ionization. .. A (Cole Parmer CPX 130; Cole-Parmer Instrument Company, Chicago, IL, USA) operating with an amplitude of 60%, maxim power of 130 W at 20 KHz, was used to generate the ultrasonic waves.

    Liquid Chromatography:

    Article Title: Herbivore-induced chemical and molecular responses of the kelps Laminaria digitata and Lessonia spicata
    Article Snippet: .. After extraction, aliquots of 50 μL were separated by ultrahigh-pressure liquid chromatography (UPLC) and analyzed by mass spectrometry (MS) on a Thermo Scientific LTQ-Orbitrap Discovery™ mass spectrometer (Thermo Scientific) equipped with an Electro Spray Ionization (ESI) source running on the negative mode, as described [ ]. .. Samples were separated using an Acclaim RSLC 120 C18 1.9 μm (2.1 x 100 mm) column 545 (Dionex; Thermo Fisher Scientific, Courtaboeuf, France) maintained at 20°C using 5 μL injection volume and a flow-rate of 250 μL.min-1 and the mobile phase (0.2% acetic acid in water–acetonitrile) was programmed from 95: 5 to 5: 95 acetonitrile–water (v: v).

    Mass Spectrometry:

    Article Title: Comparison of Proteomics Profiles of Campylobacter jejuni Strain Bf under Microaerobic and Aerobic Conditions
    Article Snippet: .. LC-MS/MS analysis was achieved on an Ultimate 3000 LC system (Dionex, Voisins-le-Bretonneux, France) linked to an LTQ-Orbitrap Discovery mass spectrometer (Thermo, USA) via a nanoelectrospray ion source. .. Samples (4 μL) were loaded at 20 μL/min onto a pre-column cartridge (stationary phase: C18 PepMap 100, 5 μm; column: 100 μm inner diameter, 1 cm in length; Thermo, France) and desalted with 2% acetonitrile −0.1% formic acid (buffer A).

    Article Title: Analysis of the Proteolytic Processing of ABCA3: Identification of Cleavage Site and Involved Proteases
    Article Snippet: .. Mass spectrometric analysis was performed on an LTQ-Orbitrap Discovery mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) operated in a data dependent mode in which each full MS scan (m/z 200–1,450) acquired in Orbitrap with resolution of R = 30,000 (m/z 400) was followed by five MS/MS scans where the five most abundant molecular ions were dynamically selected and fragmented by collision-induced dissociation (CID) using a normalized collision energy of 35% in the LTQ ion trap. ..

    Article Title: Estrogenic and cytotoxic potentials of compounds isolated from Millettia macrophylla Benth (Fabaceae): towards a better understanding of its underlying mechanisms
    Article Snippet: .. ESI-HRMS were run on a Thermo Scientific LTQ Orbitrap Discovery mass spectrometer. .. GC-MS analyses were carried out using a Hewlett-Packard 5973–6890 GC-MS system operating in the EI mode at 70 eV, equipped with an HP-5 MS capillary silica column (30 m × 0.25 mm i.d.

    Article Title: Chemical Proteomic Platform To Identify Citrullinated Proteins
    Article Snippet: .. LC-MS/MS analysis was performed on an LTQ Orbitrap Discovery mass spectrometer (ThermoFisher) coupled to an Agilent 1200 series HPLC. .. Digests were pressure loaded onto a 250 μm fused silica desalting column packed with 4 cm of Aqua C18 reverse-phase resin (Phenomenex).

    Article Title: Herbivore-induced chemical and molecular responses of the kelps Laminaria digitata and Lessonia spicata
    Article Snippet: .. After extraction, aliquots of 50 μL were separated by ultrahigh-pressure liquid chromatography (UPLC) and analyzed by mass spectrometry (MS) on a Thermo Scientific LTQ-Orbitrap Discovery™ mass spectrometer (Thermo Scientific) equipped with an Electro Spray Ionization (ESI) source running on the negative mode, as described [ ]. .. Samples were separated using an Acclaim RSLC 120 C18 1.9 μm (2.1 x 100 mm) column 545 (Dionex; Thermo Fisher Scientific, Courtaboeuf, France) maintained at 20°C using 5 μL injection volume and a flow-rate of 250 μL.min-1 and the mobile phase (0.2% acetic acid in water–acetonitrile) was programmed from 95: 5 to 5: 95 acetonitrile–water (v: v).

    Article Title: Ultrasound-promoted synthesis of 2-organoselanyl-naphthalenes using Oxone® in aqueous medium as an oxidizing agent
    Article Snippet: .. A Shimadzu GC-MS-QP2010 was used to obtain the low-resolution mass spectra (MS), while a LTQ Orbitrap Discovery mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) was employed to obtain the high-resolution mass spectra (HRMS), the experiments were performed via direct infusion of sample (flow: 10 μL/min) in the positive-ion mode using electrospray ionization. .. A (Cole Parmer CPX 130; Cole-Parmer Instrument Company, Chicago, IL, USA) operating with an amplitude of 60%, maxim power of 130 W at 20 KHz, was used to generate the ultrasonic waves.

    Article Title: Identification of deubiquitinase targets of isothiocyanates using SILAC-assisted quantitative mass spectrometry
    Article Snippet: .. LC/LC-MS/MS analysis was performed on an LTQ Orbitrap Discovery mass spectrometer (ThermoFisher) coupled to an Agilent 1200 series HPLC. .. Digests were pressure loaded onto a 250 μm fused silica desalting column packed with 4 cm of Aqua C18 reverse phase resin (Phenomenex).

    Article Title: Peptides Presented by HLA-DR Molecules in Synovia of Patients with Rheumatoid Arthritis or Antibiotic-Refractory Lyme Arthritis *
    Article Snippet: .. Analysis of post-translational modifications was conducted using accurate precursor mass measurements and collision-induced dissociation on an LTQ-Orbitrap Discovery mass spectrometer (Thermo-Fisher Scientific, Waltham, MA) that was fitted with a TriVersa NanoMate nanoelectrospray source (Advion, Ithaca, NY) and used the nanoACQUITY UPLC system with the columns and solvent gradient described above. .. Mass resolution was 60,000, and mass accuracy was within 2 ppm using external calibration against a mixture of peptide standards (Bruker Daltonics, Billerica, MA).

    Tandem Mass Spectroscopy:

    Article Title: Analysis of the Proteolytic Processing of ABCA3: Identification of Cleavage Site and Involved Proteases
    Article Snippet: .. Mass spectrometric analysis was performed on an LTQ-Orbitrap Discovery mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) operated in a data dependent mode in which each full MS scan (m/z 200–1,450) acquired in Orbitrap with resolution of R = 30,000 (m/z 400) was followed by five MS/MS scans where the five most abundant molecular ions were dynamically selected and fragmented by collision-induced dissociation (CID) using a normalized collision energy of 35% in the LTQ ion trap. ..

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    Thermo Fisher linear ion trap mass spectrometer
    MS spectra of permethylated N -linked glycans in the serum of patients with CCA compared with healthy controls, as detected using NSI-MS. Glycans released from the serum of patients with CCA and healthy controls were permethylated and analyzed. MS spectra present the predominance of the complex type and high-mannose type oligosaccharides in (A) healthy sera vs. (B) CCA sera. The glycan profiles (A vs. B) demonstrate similar glycan patterns, but they differ in their relative quantities. Glycans were detected as doubly [2+] and triply charged species [3+]. The graphical representation of monosaccharide residues are defined in the figure and are consistent with the suggested nomenclature of the Consortium for Functional Glycomics ( http://glycomics.scripps.edu/CFGnomenclature.pdf ). MS, <t>mass</t> spectrometry; CCA, cholangiocarcinoma; NSI-MS, nanospray <t>ionization-linear</t> <t>ion</t> <t>trap</t> mass spectrometry; m / z , mass/charge ratio; Glc, N-acetylglucosamine.
    Linear Ion Trap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 443 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/linear ion trap mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 443 article reviews
    Price from $9.99 to $1999.99
    linear ion trap mass spectrometer - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    90
    Thermo Fisher hybrid ltq orbitrap discovery mass spectrometer
    Base peak chromatogram of Millettia macrophylla phenolic fraction recorded in UHPLC-ESI <t>(+)-LTQ-Orbitrap</t>
    Hybrid Ltq Orbitrap Discovery Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hybrid ltq orbitrap discovery mass spectrometer/product/Thermo Fisher
    Average 90 stars, based on 91 article reviews
    Price from $9.99 to $1999.99
    hybrid ltq orbitrap discovery mass spectrometer - by Bioz Stars, 2020-05
    90/100 stars
      Buy from Supplier

    Image Search Results


    MS spectra of permethylated N -linked glycans in the serum of patients with CCA compared with healthy controls, as detected using NSI-MS. Glycans released from the serum of patients with CCA and healthy controls were permethylated and analyzed. MS spectra present the predominance of the complex type and high-mannose type oligosaccharides in (A) healthy sera vs. (B) CCA sera. The glycan profiles (A vs. B) demonstrate similar glycan patterns, but they differ in their relative quantities. Glycans were detected as doubly [2+] and triply charged species [3+]. The graphical representation of monosaccharide residues are defined in the figure and are consistent with the suggested nomenclature of the Consortium for Functional Glycomics ( http://glycomics.scripps.edu/CFGnomenclature.pdf ). MS, mass spectrometry; CCA, cholangiocarcinoma; NSI-MS, nanospray ionization-linear ion trap mass spectrometry; m / z , mass/charge ratio; Glc, N-acetylglucosamine.

    Journal: Oncology Letters

    Article Title: Increased expression of the high-mannose M6N2 and NeuAc3H3N3M3N2F tri-antennary N-glycans in cholangiocarcinoma

    doi: 10.3892/ol.2017.7384

    Figure Lengend Snippet: MS spectra of permethylated N -linked glycans in the serum of patients with CCA compared with healthy controls, as detected using NSI-MS. Glycans released from the serum of patients with CCA and healthy controls were permethylated and analyzed. MS spectra present the predominance of the complex type and high-mannose type oligosaccharides in (A) healthy sera vs. (B) CCA sera. The glycan profiles (A vs. B) demonstrate similar glycan patterns, but they differ in their relative quantities. Glycans were detected as doubly [2+] and triply charged species [3+]. The graphical representation of monosaccharide residues are defined in the figure and are consistent with the suggested nomenclature of the Consortium for Functional Glycomics ( http://glycomics.scripps.edu/CFGnomenclature.pdf ). MS, mass spectrometry; CCA, cholangiocarcinoma; NSI-MS, nanospray ionization-linear ion trap mass spectrometry; m / z , mass/charge ratio; Glc, N-acetylglucosamine.

    Article Snippet: Briefly, permethylated glycans were dissolved in 1 mM NaOH in 50% methanol and infused directly into a linear ion trap mass spectrometer (LTQ Orbitrap Discovery; Thermo Fisher Scientific, Inc., Waltham, MA, USA) using a Thermo Fisher Scientific™ nanospray ion source (Thermo Fisher Scientific, Inc.).

    Techniques: Mass Spectrometry, Functional Assay, Gas Chromatography

    Solid phase extraction-based strong cation exchange (A) Shown is the solution charge state distribution of 38,487 phosphorylated and unphosphorylated peptides from lysC digested yeast whole-cell extract subjected to the described SPE/SCX/IMAC enrichment protocol analyzed by LC-MS/MS on a LTQ Orbitrap Velos. (B) The peptide overlap between SCX fractions is shown as the fraction of all peptides that were identified in 1, 2, 3, or more different fractions. (C) A comparison of the solution charge states of the identified phosphopeptides from trypsin and lysC digested yeast whole cell extracts.

    Journal: Methods (San Diego, Calif.)

    Article Title: A solid phase extraction-based platform for rapid phosphoproteomic analysis

    doi: 10.1016/j.ymeth.2011.03.008

    Figure Lengend Snippet: Solid phase extraction-based strong cation exchange (A) Shown is the solution charge state distribution of 38,487 phosphorylated and unphosphorylated peptides from lysC digested yeast whole-cell extract subjected to the described SPE/SCX/IMAC enrichment protocol analyzed by LC-MS/MS on a LTQ Orbitrap Velos. (B) The peptide overlap between SCX fractions is shown as the fraction of all peptides that were identified in 1, 2, 3, or more different fractions. (C) A comparison of the solution charge states of the identified phosphopeptides from trypsin and lysC digested yeast whole cell extracts.

    Article Snippet: Peptides were detected in a hybrid dual-cell quadrupole linear ion trap – orbitrap mass spectrometer (LTQ Orbitrap Velos, ThermoFisher) using a data-dependent Top20 method, or the single-cell linear ion trap – orbitrap mass spectrometer (LTQ Orbitrap Discovery, ThermoFisher) using a Top10 method.

    Techniques: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry

    Base peak chromatogram of Millettia macrophylla phenolic fraction recorded in UHPLC-ESI (+)-LTQ-Orbitrap

    Journal: BMC Complementary and Alternative Medicine

    Article Title: Estrogenic and cytotoxic potentials of compounds isolated from Millettia macrophylla Benth (Fabaceae): towards a better understanding of its underlying mechanisms

    doi: 10.1186/s12906-016-1385-5

    Figure Lengend Snippet: Base peak chromatogram of Millettia macrophylla phenolic fraction recorded in UHPLC-ESI (+)-LTQ-Orbitrap

    Article Snippet: UHPLC-LTQ-Orbitrap Analysis of Millettia macrophylla phenolic fraction The analysis of the phenolic fraction of Millettia macrophylla was performed using an Accela Ultra High-Performance Liquid Chromatography (UHPLC) system equipped with a mixing pump, an autosampler, and hyphenated to a hybrid LTQ-Orbitrap Discovery Mass Spectrometer (Thermo Scientific, Bremen, Germany).

    Techniques:

    Partial sequence (162–174) is present in unprocessed ABCA3 and missing in processed protein form. A : Intensity profiles of an m/z range corresponding to the monoisotopic peak of the doubly-charged ion at m/z 819.885 ( m/z window ±5 ppm) for 190 kDa and 170 kDa gel bands acquired in Orbitrap. B : Identification of the ABCA3 tryptic peptide comprising amino acid residues 162–174 of the precursor protein and containing methionine sulfoxide at position 164. MS/MS spectra after CID fragmentation measured in the LTQ ion trap are shown. Major b and y ions are labelled on the spectra and all fragment ions obtained are marked on the identified sequence. The ion at m/z 811 corresponds to the loss of water from the parent ion.

    Journal: PLoS ONE

    Article Title: Analysis of the Proteolytic Processing of ABCA3: Identification of Cleavage Site and Involved Proteases

    doi: 10.1371/journal.pone.0152594

    Figure Lengend Snippet: Partial sequence (162–174) is present in unprocessed ABCA3 and missing in processed protein form. A : Intensity profiles of an m/z range corresponding to the monoisotopic peak of the doubly-charged ion at m/z 819.885 ( m/z window ±5 ppm) for 190 kDa and 170 kDa gel bands acquired in Orbitrap. B : Identification of the ABCA3 tryptic peptide comprising amino acid residues 162–174 of the precursor protein and containing methionine sulfoxide at position 164. MS/MS spectra after CID fragmentation measured in the LTQ ion trap are shown. Major b and y ions are labelled on the spectra and all fragment ions obtained are marked on the identified sequence. The ion at m/z 811 corresponds to the loss of water from the parent ion.

    Article Snippet: Mass spectrometric analysis was performed on an LTQ-Orbitrap Discovery mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) operated in a data dependent mode in which each full MS scan (m/z 200–1,450) acquired in Orbitrap with resolution of R = 30,000 (m/z 400) was followed by five MS/MS scans where the five most abundant molecular ions were dynamically selected and fragmented by collision-induced dissociation (CID) using a normalized collision energy of 35% in the LTQ ion trap.

    Techniques: Sequencing, Mass Spectrometry