Structured Review

New Objective Inc ltq mass spectrometer
Ltq Mass Spectrometer, supplied by New Objective Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ltq mass spectrometer/product/New Objective Inc
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
ltq mass spectrometer - by Bioz Stars, 2020-08
92/100 stars

Images

Related Articles

Chromatography:

Article Title: Proteomic analysis of the mosquito Aedes aegypti midgut brush border membrane vesicles
Article Snippet: .. Capillary-liquid chromatography-nanospray tandem mass spectrometry (Nano-LC/MS/MS) was performed on a Thermo Finnigan LTQ mass spectrometer (New Objective, Inc. Woburn, MA). .. Protein digests from each spot were applied on 5 cm, 73 μm ID ProteoPrep C-18 column (Sigma–Aldrich).

Mass Spectrometry:

Article Title: Proteomic analysis of the mosquito Aedes aegypti midgut brush border membrane vesicles
Article Snippet: .. Capillary-liquid chromatography-nanospray tandem mass spectrometry (Nano-LC/MS/MS) was performed on a Thermo Finnigan LTQ mass spectrometer (New Objective, Inc. Woburn, MA). .. Protein digests from each spot were applied on 5 cm, 73 μm ID ProteoPrep C-18 column (Sigma–Aldrich).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    New Objective Inc ltq orbitrap ms instrument
    PG-M48 Is Localized in PGs and Has Zn-Metallo-Dependent Endopeptidase Activity Cleaving Upstream of Hydrophobic Residues. (A) core proteins PGM48, ABC1K1, ABC1K3, and VTE1, as well as LHCB1 (marker for thylakoid membrane) and CPN60 (marker for stroma). Protein (15 µg) was loaded for chloroplast, stroma, and thylakoid fractions and ∼5 µg protein was loaded for PGs; due to the extremely high lipid/protein content, protein concentrations are difficult to measure accurately. The Ponceau-stained membrane (lower panel) serves as loaded control. RBCL, Rubisco large subunit (also marker for stromal proteome); LHCII, family of major LHCII proteins. (B) Recombinant PGM48 (1 µg) was incubated for 3 h at 37°C with β-casein (4 µg) to determine the peptidase activity and the effects of peptidase inhibitors E64 (1 mM), bestatin (1 mM), chymostatin (1 mM), and the metal chelator EDTA (5 mM). EDTA inhibited PGM48 peptidase activity completely. (C) ). Color coding: green: Thr, Gly, Ser, Tyr, and Cys, uncharged, polar; pink: Asn and Gln, uncharged polar amine; red: Asp and Glu, acidic; blue: Lys, Arg, and His, basic; black: Ala, Val, Leu, Ile, Pro, Phe, Trp, and Met, apolar. (D) PGM48 cleavage sites of β-casein as determined by mass spectrometry. The lower panel shows a high-resolution MS spectrum (from the <t>Orbitrap</t> portion of the <t>LTQ-Orbitrap)</t> of a casein digest by PGM48. Positively charged peptides matching to casein peptide fragments are marked; m / z values and charge states are indicated. The upper portion shows the C-terminal portion of β-casein and the identified peptides as indicated by horizontal lines with the respective m / z value and associated charge states. The inset shows the inferred cleavage sites with the β-casein sequence. Collectively, this shows that PGM48 prefers cleavage upstream of hydrophobic residues: methionine (M), phenylalanine (F), leucine (L), isoleucine (I) (in black), or tyrosine (Y) (in gray). Other colors: green: Q,S – uncharged, polar; D,E – acidic in red; K,R,H – basic in blue; remaining residues in black.
    Ltq Orbitrap Ms Instrument, supplied by New Objective Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltq orbitrap ms instrument/product/New Objective Inc
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ltq orbitrap ms instrument - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    85
    New Objective Inc ltq ft hybrid linear ion trap fourier transform ion cyclotron resonance mass spectrometer
    PG-M48 Is Localized in PGs and Has Zn-Metallo-Dependent Endopeptidase Activity Cleaving Upstream of Hydrophobic Residues. (A) core proteins PGM48, ABC1K1, ABC1K3, and VTE1, as well as LHCB1 (marker for thylakoid membrane) and CPN60 (marker for stroma). Protein (15 µg) was loaded for chloroplast, stroma, and thylakoid fractions and ∼5 µg protein was loaded for PGs; due to the extremely high lipid/protein content, protein concentrations are difficult to measure accurately. The Ponceau-stained membrane (lower panel) serves as loaded control. RBCL, Rubisco large subunit (also marker for stromal proteome); LHCII, family of major LHCII proteins. (B) Recombinant PGM48 (1 µg) was incubated for 3 h at 37°C with β-casein (4 µg) to determine the peptidase activity and the effects of peptidase inhibitors E64 (1 mM), bestatin (1 mM), chymostatin (1 mM), and the metal chelator EDTA (5 mM). EDTA inhibited PGM48 peptidase activity completely. (C) ). Color coding: green: Thr, Gly, Ser, Tyr, and Cys, uncharged, polar; pink: Asn and Gln, uncharged polar amine; red: Asp and Glu, acidic; blue: Lys, Arg, and His, basic; black: Ala, Val, Leu, Ile, Pro, Phe, Trp, and Met, apolar. (D) PGM48 cleavage sites of β-casein as determined by mass spectrometry. The lower panel shows a high-resolution MS spectrum (from the <t>Orbitrap</t> portion of the <t>LTQ-Orbitrap)</t> of a casein digest by PGM48. Positively charged peptides matching to casein peptide fragments are marked; m / z values and charge states are indicated. The upper portion shows the C-terminal portion of β-casein and the identified peptides as indicated by horizontal lines with the respective m / z value and associated charge states. The inset shows the inferred cleavage sites with the β-casein sequence. Collectively, this shows that PGM48 prefers cleavage upstream of hydrophobic residues: methionine (M), phenylalanine (F), leucine (L), isoleucine (I) (in black), or tyrosine (Y) (in gray). Other colors: green: Q,S – uncharged, polar; D,E – acidic in red; K,R,H – basic in blue; remaining residues in black.
    Ltq Ft Hybrid Linear Ion Trap Fourier Transform Ion Cyclotron Resonance Mass Spectrometer, supplied by New Objective Inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltq ft hybrid linear ion trap fourier transform ion cyclotron resonance mass spectrometer/product/New Objective Inc
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ltq ft hybrid linear ion trap fourier transform ion cyclotron resonance mass spectrometer - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    85
    New Objective Inc ltq orbitrap xl nano lc system
    PG-M48 Is Localized in PGs and Has Zn-Metallo-Dependent Endopeptidase Activity Cleaving Upstream of Hydrophobic Residues. (A) core proteins PGM48, ABC1K1, ABC1K3, and VTE1, as well as LHCB1 (marker for thylakoid membrane) and CPN60 (marker for stroma). Protein (15 µg) was loaded for chloroplast, stroma, and thylakoid fractions and ∼5 µg protein was loaded for PGs; due to the extremely high lipid/protein content, protein concentrations are difficult to measure accurately. The Ponceau-stained membrane (lower panel) serves as loaded control. RBCL, Rubisco large subunit (also marker for stromal proteome); LHCII, family of major LHCII proteins. (B) Recombinant PGM48 (1 µg) was incubated for 3 h at 37°C with β-casein (4 µg) to determine the peptidase activity and the effects of peptidase inhibitors E64 (1 mM), bestatin (1 mM), chymostatin (1 mM), and the metal chelator EDTA (5 mM). EDTA inhibited PGM48 peptidase activity completely. (C) ). Color coding: green: Thr, Gly, Ser, Tyr, and Cys, uncharged, polar; pink: Asn and Gln, uncharged polar amine; red: Asp and Glu, acidic; blue: Lys, Arg, and His, basic; black: Ala, Val, Leu, Ile, Pro, Phe, Trp, and Met, apolar. (D) PGM48 cleavage sites of β-casein as determined by mass spectrometry. The lower panel shows a high-resolution MS spectrum (from the <t>Orbitrap</t> portion of the <t>LTQ-Orbitrap)</t> of a casein digest by PGM48. Positively charged peptides matching to casein peptide fragments are marked; m / z values and charge states are indicated. The upper portion shows the C-terminal portion of β-casein and the identified peptides as indicated by horizontal lines with the respective m / z value and associated charge states. The inset shows the inferred cleavage sites with the β-casein sequence. Collectively, this shows that PGM48 prefers cleavage upstream of hydrophobic residues: methionine (M), phenylalanine (F), leucine (L), isoleucine (I) (in black), or tyrosine (Y) (in gray). Other colors: green: Q,S – uncharged, polar; D,E – acidic in red; K,R,H – basic in blue; remaining residues in black.
    Ltq Orbitrap Xl Nano Lc System, supplied by New Objective Inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltq orbitrap xl nano lc system/product/New Objective Inc
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ltq orbitrap xl nano lc system - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    88
    New Objective Inc hybrid linear ion trap mass spectrometer ltq orbitrap
    PG-M48 Is Localized in PGs and Has Zn-Metallo-Dependent Endopeptidase Activity Cleaving Upstream of Hydrophobic Residues. (A) core proteins PGM48, ABC1K1, ABC1K3, and VTE1, as well as LHCB1 (marker for thylakoid membrane) and CPN60 (marker for stroma). Protein (15 µg) was loaded for chloroplast, stroma, and thylakoid fractions and ∼5 µg protein was loaded for PGs; due to the extremely high lipid/protein content, protein concentrations are difficult to measure accurately. The Ponceau-stained membrane (lower panel) serves as loaded control. RBCL, Rubisco large subunit (also marker for stromal proteome); LHCII, family of major LHCII proteins. (B) Recombinant PGM48 (1 µg) was incubated for 3 h at 37°C with β-casein (4 µg) to determine the peptidase activity and the effects of peptidase inhibitors E64 (1 mM), bestatin (1 mM), chymostatin (1 mM), and the metal chelator EDTA (5 mM). EDTA inhibited PGM48 peptidase activity completely. (C) ). Color coding: green: Thr, Gly, Ser, Tyr, and Cys, uncharged, polar; pink: Asn and Gln, uncharged polar amine; red: Asp and Glu, acidic; blue: Lys, Arg, and His, basic; black: Ala, Val, Leu, Ile, Pro, Phe, Trp, and Met, apolar. (D) PGM48 cleavage sites of β-casein as determined by mass spectrometry. The lower panel shows a high-resolution MS spectrum (from the <t>Orbitrap</t> portion of the <t>LTQ-Orbitrap)</t> of a casein digest by PGM48. Positively charged peptides matching to casein peptide fragments are marked; m / z values and charge states are indicated. The upper portion shows the C-terminal portion of β-casein and the identified peptides as indicated by horizontal lines with the respective m / z value and associated charge states. The inset shows the inferred cleavage sites with the β-casein sequence. Collectively, this shows that PGM48 prefers cleavage upstream of hydrophobic residues: methionine (M), phenylalanine (F), leucine (L), isoleucine (I) (in black), or tyrosine (Y) (in gray). Other colors: green: Q,S – uncharged, polar; D,E – acidic in red; K,R,H – basic in blue; remaining residues in black.
    Hybrid Linear Ion Trap Mass Spectrometer Ltq Orbitrap, supplied by New Objective Inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hybrid linear ion trap mass spectrometer ltq orbitrap/product/New Objective Inc
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hybrid linear ion trap mass spectrometer ltq orbitrap - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    Image Search Results


    PG-M48 Is Localized in PGs and Has Zn-Metallo-Dependent Endopeptidase Activity Cleaving Upstream of Hydrophobic Residues. (A) core proteins PGM48, ABC1K1, ABC1K3, and VTE1, as well as LHCB1 (marker for thylakoid membrane) and CPN60 (marker for stroma). Protein (15 µg) was loaded for chloroplast, stroma, and thylakoid fractions and ∼5 µg protein was loaded for PGs; due to the extremely high lipid/protein content, protein concentrations are difficult to measure accurately. The Ponceau-stained membrane (lower panel) serves as loaded control. RBCL, Rubisco large subunit (also marker for stromal proteome); LHCII, family of major LHCII proteins. (B) Recombinant PGM48 (1 µg) was incubated for 3 h at 37°C with β-casein (4 µg) to determine the peptidase activity and the effects of peptidase inhibitors E64 (1 mM), bestatin (1 mM), chymostatin (1 mM), and the metal chelator EDTA (5 mM). EDTA inhibited PGM48 peptidase activity completely. (C) ). Color coding: green: Thr, Gly, Ser, Tyr, and Cys, uncharged, polar; pink: Asn and Gln, uncharged polar amine; red: Asp and Glu, acidic; blue: Lys, Arg, and His, basic; black: Ala, Val, Leu, Ile, Pro, Phe, Trp, and Met, apolar. (D) PGM48 cleavage sites of β-casein as determined by mass spectrometry. The lower panel shows a high-resolution MS spectrum (from the Orbitrap portion of the LTQ-Orbitrap) of a casein digest by PGM48. Positively charged peptides matching to casein peptide fragments are marked; m / z values and charge states are indicated. The upper portion shows the C-terminal portion of β-casein and the identified peptides as indicated by horizontal lines with the respective m / z value and associated charge states. The inset shows the inferred cleavage sites with the β-casein sequence. Collectively, this shows that PGM48 prefers cleavage upstream of hydrophobic residues: methionine (M), phenylalanine (F), leucine (L), isoleucine (I) (in black), or tyrosine (Y) (in gray). Other colors: green: Q,S – uncharged, polar; D,E – acidic in red; K,R,H – basic in blue; remaining residues in black.

    Journal: The Plant Cell

    Article Title: The Plastoglobule-Localized Metallopeptidase PGM48 Is a Positive Regulator of Senescence in Arabidopsis thaliana

    doi: 10.1105/tpc.16.00745

    Figure Lengend Snippet: PG-M48 Is Localized in PGs and Has Zn-Metallo-Dependent Endopeptidase Activity Cleaving Upstream of Hydrophobic Residues. (A) core proteins PGM48, ABC1K1, ABC1K3, and VTE1, as well as LHCB1 (marker for thylakoid membrane) and CPN60 (marker for stroma). Protein (15 µg) was loaded for chloroplast, stroma, and thylakoid fractions and ∼5 µg protein was loaded for PGs; due to the extremely high lipid/protein content, protein concentrations are difficult to measure accurately. The Ponceau-stained membrane (lower panel) serves as loaded control. RBCL, Rubisco large subunit (also marker for stromal proteome); LHCII, family of major LHCII proteins. (B) Recombinant PGM48 (1 µg) was incubated for 3 h at 37°C with β-casein (4 µg) to determine the peptidase activity and the effects of peptidase inhibitors E64 (1 mM), bestatin (1 mM), chymostatin (1 mM), and the metal chelator EDTA (5 mM). EDTA inhibited PGM48 peptidase activity completely. (C) ). Color coding: green: Thr, Gly, Ser, Tyr, and Cys, uncharged, polar; pink: Asn and Gln, uncharged polar amine; red: Asp and Glu, acidic; blue: Lys, Arg, and His, basic; black: Ala, Val, Leu, Ile, Pro, Phe, Trp, and Met, apolar. (D) PGM48 cleavage sites of β-casein as determined by mass spectrometry. The lower panel shows a high-resolution MS spectrum (from the Orbitrap portion of the LTQ-Orbitrap) of a casein digest by PGM48. Positively charged peptides matching to casein peptide fragments are marked; m / z values and charge states are indicated. The upper portion shows the C-terminal portion of β-casein and the identified peptides as indicated by horizontal lines with the respective m / z value and associated charge states. The inset shows the inferred cleavage sites with the β-casein sequence. Collectively, this shows that PGM48 prefers cleavage upstream of hydrophobic residues: methionine (M), phenylalanine (F), leucine (L), isoleucine (I) (in black), or tyrosine (Y) (in gray). Other colors: green: Q,S – uncharged, polar; D,E – acidic in red; K,R,H – basic in blue; remaining residues in black.

    Article Snippet: The peptide mixture was directly infused into a LTQ-Orbitrap MS instrument at a flow rate of 0.3 μL per minute through a 15-µm fused silica emitter tip (New Objective).

    Techniques: Activity Assay, Marker, Staining, Recombinant, Incubation, Mass Spectrometry, Sequencing