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Agilent technologies ltq mass spectrometer
Ltq Mass Spectrometer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ltq mass spectrometer/product/Agilent technologies
Average 92 stars, based on 6 article reviews
Price from $9.99 to $1999.99
ltq mass spectrometer - by Bioz Stars, 2020-05
92/100 stars

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High Performance Liquid Chromatography:

Article Title: A Shotgun Proteomic Method for the Identification of Membrane-Embedded Proteins and Peptides
Article Snippet: .. After desalting with Buffer A, the 2-phase separation column with attached desalting column was placed in-line with an LTQ mass spectrometer and interfaced with an Agilent 1100 binary HPLC and autosampler system. ..

Article Title: A Comprehensive Defensin Assay for Saliva
Article Snippet: .. LC-MS/MS experiments were performed using a Thermo-Electron (San Jose, CA) LTQ mass spectrometer with an Agilent 1200 HPLC system. .. Chromatographic separation was conducted using a Zorbax SB-C3, 2.1×150mm, 5 mm particle column (Agilent Technologies, Santa Clara, CA, USA).

Article Title: On protein abundance distributions in complex mixtures
Article Snippet: .. The loaded samples were directly introduced into the LTQ mass spectrometer equipped with ESI nanospray ion source by eluting the bound peptides with a 2D-LC-MS/MS scheme controlled by Agilent 1100 HPLC quaternary pump [ ]. ..

Flow Cytometry:

Article Title: Regulation of Protein Interactions by Mps One Binder (MOB1) Phosphorylation *
Article Snippet: .. The loaded column was then placed in-line with a LTQ mass spectrometer equipped with an Agilent 1100 pump with split flow and a Proxeon source. ..

Mass Spectrometry:

Article Title: A Shotgun Proteomic Method for the Identification of Membrane-Embedded Proteins and Peptides
Article Snippet: .. After desalting with Buffer A, the 2-phase separation column with attached desalting column was placed in-line with an LTQ mass spectrometer and interfaced with an Agilent 1100 binary HPLC and autosampler system. ..

Article Title: Analysis of Myosin Heavy Chain Isoforms from Longissimus Thoracis Muscle of Hanwoo Steer by Electrophoresis and LC-MS/MS
Article Snippet: .. LC-MS/MS and data searching LC-MS/MS analysis was performed with a nano-LC and LTQ mass spectrometer (Agilent 1100, Thermo Electon, USA). .. The capillary column was obtained from Proxeon (150 mm × 0.075 mm, Odense M, Denmark) and slurry packed in-house with 5 μm, 100 Å pore size Magic C18 stationary phase (Michrom Bioresources, USA).

Article Title: A Comprehensive Defensin Assay for Saliva
Article Snippet: .. LC-MS/MS experiments were performed using a Thermo-Electron (San Jose, CA) LTQ mass spectrometer with an Agilent 1200 HPLC system. .. Chromatographic separation was conducted using a Zorbax SB-C3, 2.1×150mm, 5 mm particle column (Agilent Technologies, Santa Clara, CA, USA).

Article Title: On protein abundance distributions in complex mixtures
Article Snippet: .. The loaded samples were directly introduced into the LTQ mass spectrometer equipped with ESI nanospray ion source by eluting the bound peptides with a 2D-LC-MS/MS scheme controlled by Agilent 1100 HPLC quaternary pump [ ]. ..

Article Title: Mesenchymal stem cells infected with Mycoplasma arginini secrete complement C3 to regulate immunoglobulin production in b lymphocytes
Article Snippet: .. Nano-LC-tandem mass spectroscopy (MS/MS) analysis was performed on an Agilent 1100 series nano-LC and LTQ-mass spectrometer (Agilent, Santa Clara, CA, USA). ..

Tandem Mass Spectroscopy:

Article Title: Mesenchymal stem cells infected with Mycoplasma arginini secrete complement C3 to regulate immunoglobulin production in b lymphocytes
Article Snippet: .. Nano-LC-tandem mass spectroscopy (MS/MS) analysis was performed on an Agilent 1100 series nano-LC and LTQ-mass spectrometer (Agilent, Santa Clara, CA, USA). ..

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    Agilent technologies nano lc ltq ms ms setup
    HPLC separation in combination with mass spectrometry for Embp identification. The proteins in the culture supernatants of S. epidermidis (ATCC12228) were separated by reversed-phase HPLC using a LUNA C18 5 μm column. ( a ) Twelve fractions were collected after HPLC separation. The HI activity of the eluted proteins (100 μl) in each fraction was tested using A/San Diego/1/09(H1N1 pdm09) as the virus antigen. ( b ) Tryptic digests of proteins in fraction 12 of the HLPC separation, which showed the greatest HI activity, were subjected to <t>Nano-LC-LTQ</t> MS/MS. A sequenced peptide (SINAYNKAIQSLETQITSAKDN) is shown and was identified as an internal peptide of Embp (Q8CP76). The m/z value of each “y” and “b” ion in collision-induced dissociation (CID) spectra is indicated.
    Nano Lc Ltq Ms Ms Setup, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nano lc ltq ms ms setup/product/Agilent technologies
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nano lc ltq ms ms setup - by Bioz Stars, 2020-05
    90/100 stars
      Buy from Supplier

    92
    Agilent technologies ltq orbitrap velos mass spectrometer
    Solid phase extraction-based strong cation exchange (A) Shown is the solution charge state distribution of 38,487 phosphorylated and unphosphorylated peptides from lysC digested yeast whole-cell extract subjected to the described SPE/SCX/IMAC enrichment protocol analyzed by LC-MS/MS on a <t>LTQ</t> <t>Orbitrap</t> <t>Velos.</t> (B) The peptide overlap between SCX fractions is shown as the fraction of all peptides that were identified in 1, 2, 3, or more different fractions. (C) A comparison of the solution charge states of the identified phosphopeptides from trypsin and lysC digested yeast whole cell extracts.
    Ltq Orbitrap Velos Mass Spectrometer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltq orbitrap velos mass spectrometer/product/Agilent technologies
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ltq orbitrap velos mass spectrometer - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    93
    Agilent technologies hplc ltq orbitrap ms analysis
    The total ion chromatograms (TIC) of the total flavonoids of P. pulchellum by high-performance liquid chromatography-linear ion trap <t>quadrupole-Orbitrap-mass</t> spectrometry <t>(HPLC-LTQ-Orbitrap-MS)</t> in negative ion mode ( A ) and positive ion mode ( B ). The compounds that are confirmed with the isolated reference compounds are marked in red.
    Hplc Ltq Orbitrap Ms Analysis, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc ltq orbitrap ms analysis/product/Agilent technologies
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    hplc ltq orbitrap ms analysis - by Bioz Stars, 2020-05
    93/100 stars
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    92
    Agilent technologies ltq orbitrap xl mass spectrometer
    Proteomics approach used for phosphotyrosine profiling of E. coli . EHEC O157:H7 strain TUV93-0 and E. coli K12 strain MG1655 were grown to stationary phase in DMEM. Total protein was trypsin-digested, peptides purified using reversed-phased chromatography and subjected to immunoaffinity (IP)-based phosphotyrosine enrichment. Tyrosine phosphorylated peptides were identified by high resolution LC-MS/MS analysis using <t>LTQ</t> <t>Orbitrap</t> XL and Velos instruments.
    Ltq Orbitrap Xl Mass Spectrometer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltq orbitrap xl mass spectrometer/product/Agilent technologies
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    ltq orbitrap xl mass spectrometer - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

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    HPLC separation in combination with mass spectrometry for Embp identification. The proteins in the culture supernatants of S. epidermidis (ATCC12228) were separated by reversed-phase HPLC using a LUNA C18 5 μm column. ( a ) Twelve fractions were collected after HPLC separation. The HI activity of the eluted proteins (100 μl) in each fraction was tested using A/San Diego/1/09(H1N1 pdm09) as the virus antigen. ( b ) Tryptic digests of proteins in fraction 12 of the HLPC separation, which showed the greatest HI activity, were subjected to Nano-LC-LTQ MS/MS. A sequenced peptide (SINAYNKAIQSLETQITSAKDN) is shown and was identified as an internal peptide of Embp (Q8CP76). The m/z value of each “y” and “b” ion in collision-induced dissociation (CID) spectra is indicated.

    Journal: Scientific Reports

    Article Title: Nasal commensal Staphylococcus epidermidis counteracts influenza virus

    doi: 10.1038/srep27870

    Figure Lengend Snippet: HPLC separation in combination with mass spectrometry for Embp identification. The proteins in the culture supernatants of S. epidermidis (ATCC12228) were separated by reversed-phase HPLC using a LUNA C18 5 μm column. ( a ) Twelve fractions were collected after HPLC separation. The HI activity of the eluted proteins (100 μl) in each fraction was tested using A/San Diego/1/09(H1N1 pdm09) as the virus antigen. ( b ) Tryptic digests of proteins in fraction 12 of the HLPC separation, which showed the greatest HI activity, were subjected to Nano-LC-LTQ MS/MS. A sequenced peptide (SINAYNKAIQSLETQITSAKDN) is shown and was identified as an internal peptide of Embp (Q8CP76). The m/z value of each “y” and “b” ion in collision-induced dissociation (CID) spectra is indicated.

    Article Snippet: The automated Nano-LC-LTQ MS/MS setup consisted of an Eksigent Nano 2D LC system, a switch valve, a C18 trap column (Agilent, Santa Clara, CA), and a capillary reversed-phase column (10 cm in length, 75 mm internal diameter) packed with 5 mm, C18 AQUASIL resin with an integral spray tip (Picofrit, 15 mm tip, New Objective, Woburn, MA).

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry, Activity Assay

    Solid phase extraction-based strong cation exchange (A) Shown is the solution charge state distribution of 38,487 phosphorylated and unphosphorylated peptides from lysC digested yeast whole-cell extract subjected to the described SPE/SCX/IMAC enrichment protocol analyzed by LC-MS/MS on a LTQ Orbitrap Velos. (B) The peptide overlap between SCX fractions is shown as the fraction of all peptides that were identified in 1, 2, 3, or more different fractions. (C) A comparison of the solution charge states of the identified phosphopeptides from trypsin and lysC digested yeast whole cell extracts.

    Journal: Methods (San Diego, Calif.)

    Article Title: A solid phase extraction-based platform for rapid phosphoproteomic analysis

    doi: 10.1016/j.ymeth.2011.03.008

    Figure Lengend Snippet: Solid phase extraction-based strong cation exchange (A) Shown is the solution charge state distribution of 38,487 phosphorylated and unphosphorylated peptides from lysC digested yeast whole-cell extract subjected to the described SPE/SCX/IMAC enrichment protocol analyzed by LC-MS/MS on a LTQ Orbitrap Velos. (B) The peptide overlap between SCX fractions is shown as the fraction of all peptides that were identified in 1, 2, 3, or more different fractions. (C) A comparison of the solution charge states of the identified phosphopeptides from trypsin and lysC digested yeast whole cell extracts.

    Article Snippet: For all experiments, a single replicate of seven samples was analyzed on a LTQ Orbitrap Velos mass spectrometer equipped with an Agilent 1100 binary pump (#G1312A) or a LTQ Orbitrap Discovery equipped with a Thermo Surveyor MS Pump (ThermoFisher).

    Techniques: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry

    The total ion chromatograms (TIC) of the total flavonoids of P. pulchellum by high-performance liquid chromatography-linear ion trap quadrupole-Orbitrap-mass spectrometry (HPLC-LTQ-Orbitrap-MS) in negative ion mode ( A ) and positive ion mode ( B ). The compounds that are confirmed with the isolated reference compounds are marked in red.

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: Phytochemical Composition, Hepatoprotective, and Antioxidant Activities of Phyllodium pulchellum (L.) Desv

    doi: 10.3390/molecules23061361

    Figure Lengend Snippet: The total ion chromatograms (TIC) of the total flavonoids of P. pulchellum by high-performance liquid chromatography-linear ion trap quadrupole-Orbitrap-mass spectrometry (HPLC-LTQ-Orbitrap-MS) in negative ion mode ( A ) and positive ion mode ( B ). The compounds that are confirmed with the isolated reference compounds are marked in red.

    Article Snippet: The HPLC-LTQ-Orbitrap-MS analysis was performed on an Agilent series 1290 Infinity HPLC instrument (Agilent, Technologies, Santa Clara, CA, USA), coupled with an LTQ/Orbitrap mass spectrometer (Thermo Scientific, Bremen, Germany) that was equipped with an electrospray (ESI) interface.

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry, Isolation

    Proteomics approach used for phosphotyrosine profiling of E. coli . EHEC O157:H7 strain TUV93-0 and E. coli K12 strain MG1655 were grown to stationary phase in DMEM. Total protein was trypsin-digested, peptides purified using reversed-phased chromatography and subjected to immunoaffinity (IP)-based phosphotyrosine enrichment. Tyrosine phosphorylated peptides were identified by high resolution LC-MS/MS analysis using LTQ Orbitrap XL and Velos instruments.

    Journal: PLoS Pathogens

    Article Title: The Escherichia coli Phosphotyrosine Proteome Relates to Core Pathways and Virulence

    doi: 10.1371/journal.ppat.1003403

    Figure Lengend Snippet: Proteomics approach used for phosphotyrosine profiling of E. coli . EHEC O157:H7 strain TUV93-0 and E. coli K12 strain MG1655 were grown to stationary phase in DMEM. Total protein was trypsin-digested, peptides purified using reversed-phased chromatography and subjected to immunoaffinity (IP)-based phosphotyrosine enrichment. Tyrosine phosphorylated peptides were identified by high resolution LC-MS/MS analysis using LTQ Orbitrap XL and Velos instruments.

    Article Snippet: The LTQ Orbitrap XL mass spectrometer was equipped with a nanoflow electrospray system connected to Eksigent nanoliquid chromatography and an Agilent 1100 microwell plate autosampler.

    Techniques: Purification, Chromatography, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry