ltq lc ms instrument  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ltq lc ms instrument
    Production of IAA in ipdC -dependent manner by Salmonella enterica sv. Typhimuirum 14028. (A) Detection of IAA by the Salkowski reagent. Results are from replicates from three independent experiments, averages are shown. Error bars are standard deviations. (B) HPLC detection of IAA and tryptophol in spent cultures of the wild type and the ipdC mutant. Hydrophobic fractions of Salmonella culture filtrates were subjected to reverse phase (C 18 ) liquid chromatography, eluted isocratically with acidified water/methanol and eluting substances were detected with a UV/VIS detector set to 230 and 280 nm. (C) Low Resolution <t>Electrospray</t> Ionization LC-MS of the hydrophobic fraction of Salmonella culture filtrates. <t>LTQ</t> LC-MS was carried out using a Grace Vydac 218TP C18 and acetonitrile/water gradient. (D) Expression of the ipdC RIVET reporter in the wild type and Δ ipdC backgrounds was measured in Minimal A medium with and without synthetic IAA or tryptophan in cultures incubated at 22°C for 72 h. Samples were streaked to xylose lysine deoxycholate (XLD) agar with kanamycin at 24 and 72 h. Plates were incubated at 37°C overnight and colonies were patched to LB agar with tetracycline to quantify resolution. Experiments were repeated five times (without technical replications), averages from the five experiments are shown. Error bars are standard deviations.
    Ltq Lc Ms Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltq lc ms instrument/product/Thermo Fisher
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ltq lc ms instrument - by Bioz Stars, 2020-09
    92/100 stars

    Images

    1) Product Images from "Production of the Plant Hormone Auxin by Salmonella and Its Role in the Interactions with Plants and Animals"

    Article Title: Production of the Plant Hormone Auxin by Salmonella and Its Role in the Interactions with Plants and Animals

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2017.02668

    Production of IAA in ipdC -dependent manner by Salmonella enterica sv. Typhimuirum 14028. (A) Detection of IAA by the Salkowski reagent. Results are from replicates from three independent experiments, averages are shown. Error bars are standard deviations. (B) HPLC detection of IAA and tryptophol in spent cultures of the wild type and the ipdC mutant. Hydrophobic fractions of Salmonella culture filtrates were subjected to reverse phase (C 18 ) liquid chromatography, eluted isocratically with acidified water/methanol and eluting substances were detected with a UV/VIS detector set to 230 and 280 nm. (C) Low Resolution Electrospray Ionization LC-MS of the hydrophobic fraction of Salmonella culture filtrates. LTQ LC-MS was carried out using a Grace Vydac 218TP C18 and acetonitrile/water gradient. (D) Expression of the ipdC RIVET reporter in the wild type and Δ ipdC backgrounds was measured in Minimal A medium with and without synthetic IAA or tryptophan in cultures incubated at 22°C for 72 h. Samples were streaked to xylose lysine deoxycholate (XLD) agar with kanamycin at 24 and 72 h. Plates were incubated at 37°C overnight and colonies were patched to LB agar with tetracycline to quantify resolution. Experiments were repeated five times (without technical replications), averages from the five experiments are shown. Error bars are standard deviations.
    Figure Legend Snippet: Production of IAA in ipdC -dependent manner by Salmonella enterica sv. Typhimuirum 14028. (A) Detection of IAA by the Salkowski reagent. Results are from replicates from three independent experiments, averages are shown. Error bars are standard deviations. (B) HPLC detection of IAA and tryptophol in spent cultures of the wild type and the ipdC mutant. Hydrophobic fractions of Salmonella culture filtrates were subjected to reverse phase (C 18 ) liquid chromatography, eluted isocratically with acidified water/methanol and eluting substances were detected with a UV/VIS detector set to 230 and 280 nm. (C) Low Resolution Electrospray Ionization LC-MS of the hydrophobic fraction of Salmonella culture filtrates. LTQ LC-MS was carried out using a Grace Vydac 218TP C18 and acetonitrile/water gradient. (D) Expression of the ipdC RIVET reporter in the wild type and Δ ipdC backgrounds was measured in Minimal A medium with and without synthetic IAA or tryptophan in cultures incubated at 22°C for 72 h. Samples were streaked to xylose lysine deoxycholate (XLD) agar with kanamycin at 24 and 72 h. Plates were incubated at 37°C overnight and colonies were patched to LB agar with tetracycline to quantify resolution. Experiments were repeated five times (without technical replications), averages from the five experiments are shown. Error bars are standard deviations.

    Techniques Used: High Performance Liquid Chromatography, Mutagenesis, Liquid Chromatography, Liquid Chromatography with Mass Spectroscopy, Expressing, Incubation

    2) Product Images from "Production of the Plant Hormone Auxin by Salmonella and Its Role in the Interactions with Plants and Animals"

    Article Title: Production of the Plant Hormone Auxin by Salmonella and Its Role in the Interactions with Plants and Animals

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2017.02668

    Production of IAA in ipdC -dependent manner by Salmonella enterica sv. Typhimuirum 14028. (A) Detection of IAA by the Salkowski reagent. Results are from replicates from three independent experiments, averages are shown. Error bars are standard deviations. (B) HPLC detection of IAA and tryptophol in spent cultures of the wild type and the ipdC mutant. Hydrophobic fractions of Salmonella culture filtrates were subjected to reverse phase (C 18 ) liquid chromatography, eluted isocratically with acidified water/methanol and eluting substances were detected with a UV/VIS detector set to 230 and 280 nm. (C) Low Resolution Electrospray Ionization LC-MS of the hydrophobic fraction of Salmonella culture filtrates. LTQ LC-MS was carried out using a Grace Vydac 218TP C18 and acetonitrile/water gradient. (D) Expression of the ipdC RIVET reporter in the wild type and Δ ipdC backgrounds was measured in Minimal A medium with and without synthetic IAA or tryptophan in cultures incubated at 22°C for 72 h. Samples were streaked to xylose lysine deoxycholate (XLD) agar with kanamycin at 24 and 72 h. Plates were incubated at 37°C overnight and colonies were patched to LB agar with tetracycline to quantify resolution. Experiments were repeated five times (without technical replications), averages from the five experiments are shown. Error bars are standard deviations.
    Figure Legend Snippet: Production of IAA in ipdC -dependent manner by Salmonella enterica sv. Typhimuirum 14028. (A) Detection of IAA by the Salkowski reagent. Results are from replicates from three independent experiments, averages are shown. Error bars are standard deviations. (B) HPLC detection of IAA and tryptophol in spent cultures of the wild type and the ipdC mutant. Hydrophobic fractions of Salmonella culture filtrates were subjected to reverse phase (C 18 ) liquid chromatography, eluted isocratically with acidified water/methanol and eluting substances were detected with a UV/VIS detector set to 230 and 280 nm. (C) Low Resolution Electrospray Ionization LC-MS of the hydrophobic fraction of Salmonella culture filtrates. LTQ LC-MS was carried out using a Grace Vydac 218TP C18 and acetonitrile/water gradient. (D) Expression of the ipdC RIVET reporter in the wild type and Δ ipdC backgrounds was measured in Minimal A medium with and without synthetic IAA or tryptophan in cultures incubated at 22°C for 72 h. Samples were streaked to xylose lysine deoxycholate (XLD) agar with kanamycin at 24 and 72 h. Plates were incubated at 37°C overnight and colonies were patched to LB agar with tetracycline to quantify resolution. Experiments were repeated five times (without technical replications), averages from the five experiments are shown. Error bars are standard deviations.

    Techniques Used: High Performance Liquid Chromatography, Mutagenesis, Liquid Chromatography, Liquid Chromatography with Mass Spectroscopy, Expressing, Incubation

    Related Articles

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Production of the Plant Hormone Auxin by Salmonella and Its Role in the Interactions with Plants and Animals
    Article Snippet: .. Low Resolution Electrospray Ionization LCMS was carried in a Thermo Scientific LTQ LC-MS instrument using a Grace Vydac 218TP C18 (5 μ, 7.5 cm, 2.1 mm ID) column. .. LCMS was run using a gradient of 10% acetonitrile (with 0.1% formic acid) and 90% water (with 0.1% formic acid) to 100% acetonitrile (with 0.1% formic acid) from 0 to 15 min and then 100% acetonitrile with formic acid was continued till 21 min.

    Article Title: A Maldiisotopic Approach to Discover Natural Products: Cryptomaldamide, a Hybrid Tripeptide from the Marine Cyanobacterium Moorea producens
    Article Snippet: .. LC-MS data was obtained on a Thermo-Electron LTQ LC/MS instrument with a Phenomenex Luna 5 μm C18(2) 100 Å column (4.6 × 250 mm) and a gradient starting at 60% MeCN/40% H2 O and immediately ramping to 100% MeCN over 20 min, then holding at 100% MeCN for 5 min. MS fragmentation experiments were obtained using a Biversa Nanomate (Advion Biosystems) electrospray source for a Finnigan LTQ-FTICR-MS instrument (Thermo-Electron Corporation) running Tune Plus software version 1.0. .. HPLC purification was carried out with a Waters 515 HPLC pump with a Waters 996 photodiode array detector using Empower Pro software and a Phenomenex Synergi 4 μm C18 column (10 × 250 mm) and a gradient starting at 50:50 MeCN/H2 O for 10 min, ramping to 100% MeCN over 12 min and holding for 3 min. All solvents were HPLC grade.

    Mass Spectrometry:

    Article Title: A Maldiisotopic Approach to Discover Natural Products: Cryptomaldamide, a Hybrid Tripeptide from the Marine Cyanobacterium Moorea producens
    Article Snippet: .. LC-MS data was obtained on a Thermo-Electron LTQ LC/MS instrument with a Phenomenex Luna 5 μm C18(2) 100 Å column (4.6 × 250 mm) and a gradient starting at 60% MeCN/40% H2 O and immediately ramping to 100% MeCN over 20 min, then holding at 100% MeCN for 5 min. MS fragmentation experiments were obtained using a Biversa Nanomate (Advion Biosystems) electrospray source for a Finnigan LTQ-FTICR-MS instrument (Thermo-Electron Corporation) running Tune Plus software version 1.0. .. HPLC purification was carried out with a Waters 515 HPLC pump with a Waters 996 photodiode array detector using Empower Pro software and a Phenomenex Synergi 4 μm C18 column (10 × 250 mm) and a gradient starting at 50:50 MeCN/H2 O for 10 min, ramping to 100% MeCN over 12 min and holding for 3 min. All solvents were HPLC grade.

    Software:

    Article Title: A Maldiisotopic Approach to Discover Natural Products: Cryptomaldamide, a Hybrid Tripeptide from the Marine Cyanobacterium Moorea producens
    Article Snippet: .. LC-MS data was obtained on a Thermo-Electron LTQ LC/MS instrument with a Phenomenex Luna 5 μm C18(2) 100 Å column (4.6 × 250 mm) and a gradient starting at 60% MeCN/40% H2 O and immediately ramping to 100% MeCN over 20 min, then holding at 100% MeCN for 5 min. MS fragmentation experiments were obtained using a Biversa Nanomate (Advion Biosystems) electrospray source for a Finnigan LTQ-FTICR-MS instrument (Thermo-Electron Corporation) running Tune Plus software version 1.0. .. HPLC purification was carried out with a Waters 515 HPLC pump with a Waters 996 photodiode array detector using Empower Pro software and a Phenomenex Synergi 4 μm C18 column (10 × 250 mm) and a gradient starting at 50:50 MeCN/H2 O for 10 min, ramping to 100% MeCN over 12 min and holding for 3 min. All solvents were HPLC grade.

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    Thermo Fisher ltq velos pro linear ion trap mass spectrometer
    PGC-MS analysis of bi-functionalized cello-oligosaccharides. The oxime bi-functionalized oligosaccharides were separated by porous graphitized carbon (PGC) chromatography using an <t>LTQ</t> <t>Velos</t> Pro mass spectrometer (Thermo) for detection. ( A ) Overlay of three extracted ion chromatograms for m/z -ratios 619.25, 781.28 and 943.35, showing separation of the bi-functionalized products with DP 3, 4 and 5, respectively (note that these are proton adducts). The dashed line shows the time of recording of the MS/MS spectra. ( B ) MS/MS spectra of the three products show a dominant cleavage of the glycosidic bonds followed by multiple water losses (annotated as B-(H 2 O) 2 ). A cleavage of the reducing-end amine linker bond can also be seen resulting in the following fragments; 543.17, 705.23 and 867.29 for the mother ions 619.25, 781.28 and 943.35, respectively. ( C ) Structures of the three bi-functionalized products, with theoretical masses and the observed fragmentation patterns (using the nomenclature of Domon and Costello) 49 .
    Ltq Velos Pro Linear Ion Trap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltq velos pro linear ion trap mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ltq velos pro linear ion trap mass spectrometer - by Bioz Stars, 2020-09
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    92
    Thermo Fisher ltq lc ms instrument
    Production of IAA in ipdC -dependent manner by Salmonella enterica sv. Typhimuirum 14028. (A) Detection of IAA by the Salkowski reagent. Results are from replicates from three independent experiments, averages are shown. Error bars are standard deviations. (B) HPLC detection of IAA and tryptophol in spent cultures of the wild type and the ipdC mutant. Hydrophobic fractions of Salmonella culture filtrates were subjected to reverse phase (C 18 ) liquid chromatography, eluted isocratically with acidified water/methanol and eluting substances were detected with a UV/VIS detector set to 230 and 280 nm. (C) Low Resolution <t>Electrospray</t> Ionization LC-MS of the hydrophobic fraction of Salmonella culture filtrates. <t>LTQ</t> LC-MS was carried out using a Grace Vydac 218TP C18 and acetonitrile/water gradient. (D) Expression of the ipdC RIVET reporter in the wild type and Δ ipdC backgrounds was measured in Minimal A medium with and without synthetic IAA or tryptophan in cultures incubated at 22°C for 72 h. Samples were streaked to xylose lysine deoxycholate (XLD) agar with kanamycin at 24 and 72 h. Plates were incubated at 37°C overnight and colonies were patched to LB agar with tetracycline to quantify resolution. Experiments were repeated five times (without technical replications), averages from the five experiments are shown. Error bars are standard deviations.
    Ltq Lc Ms Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltq lc ms instrument/product/Thermo Fisher
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ltq lc ms instrument - by Bioz Stars, 2020-09
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    99
    Thermo Fisher linear ion trap orbitrap mass spectrometer
    Influence of the potential applied to the ESI source on retention of UDP-sugars. Chromatogram obtained with a newly installed or regenerated PGC column ( a ); chromatogram obtained after 20-min utilization of the column connected to the ESI source of an <t>Orbitrap</t> mass spectrometer ( b ). Conditions: column regeneration was performed with 80 % acetonitrile in water containing 0.10 % trifluoroacetic acid for 20 min, the sample measurements were done with the gradient program described in the materials and methods section for HPLC-MS; detection, UV, 262 nm; sample. UDP-Gal (100 μmol L −1 ) and UDP-Glc (100 μmol L −1 )
    Linear Ion Trap Orbitrap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/linear ion trap orbitrap mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 55 article reviews
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    Image Search Results


    PGC-MS analysis of bi-functionalized cello-oligosaccharides. The oxime bi-functionalized oligosaccharides were separated by porous graphitized carbon (PGC) chromatography using an LTQ Velos Pro mass spectrometer (Thermo) for detection. ( A ) Overlay of three extracted ion chromatograms for m/z -ratios 619.25, 781.28 and 943.35, showing separation of the bi-functionalized products with DP 3, 4 and 5, respectively (note that these are proton adducts). The dashed line shows the time of recording of the MS/MS spectra. ( B ) MS/MS spectra of the three products show a dominant cleavage of the glycosidic bonds followed by multiple water losses (annotated as B-(H 2 O) 2 ). A cleavage of the reducing-end amine linker bond can also be seen resulting in the following fragments; 543.17, 705.23 and 867.29 for the mother ions 619.25, 781.28 and 943.35, respectively. ( C ) Structures of the three bi-functionalized products, with theoretical masses and the observed fragmentation patterns (using the nomenclature of Domon and Costello) 49 .

    Journal: Scientific Reports

    Article Title: Synthesis of glycoconjugates utilizing the regioselectivity of a lytic polysaccharide monooxygenase

    doi: 10.1038/s41598-020-69951-7

    Figure Lengend Snippet: PGC-MS analysis of bi-functionalized cello-oligosaccharides. The oxime bi-functionalized oligosaccharides were separated by porous graphitized carbon (PGC) chromatography using an LTQ Velos Pro mass spectrometer (Thermo) for detection. ( A ) Overlay of three extracted ion chromatograms for m/z -ratios 619.25, 781.28 and 943.35, showing separation of the bi-functionalized products with DP 3, 4 and 5, respectively (note that these are proton adducts). The dashed line shows the time of recording of the MS/MS spectra. ( B ) MS/MS spectra of the three products show a dominant cleavage of the glycosidic bonds followed by multiple water losses (annotated as B-(H 2 O) 2 ). A cleavage of the reducing-end amine linker bond can also be seen resulting in the following fragments; 543.17, 705.23 and 867.29 for the mother ions 619.25, 781.28 and 943.35, respectively. ( C ) Structures of the three bi-functionalized products, with theoretical masses and the observed fragmentation patterns (using the nomenclature of Domon and Costello) 49 .

    Article Snippet: Mass spectrometry of oligosaccharides, direct injection MS, PGC-MS and HILIC-FLD-MS For direct injection, oligosaccharides were analyzed using an LTQ-Velos Pro linear ion trap mass spectrometer (Thermo Scientific, San Jose, CA, USA) connected to an Ultimate 3000RS HPLC (Dionex, Sunnyvale, CA, USA).

    Techniques: Pyrolysis Gas Chromatography, Chromatography, Mass Spectrometry, Tandem Mass Spectroscopy

    PGC-MS analysis of bi-functionalized cello-oligosaccharides. The oxime bi-functionalized oligosaccharides were separated by porous graphitized carbon (PGC) chromatography using an LTQ Velos Pro mass spectrometer (Thermo) for detection. A) Overlay of three extracted ion chromatograms for m/z -ratios 619.25, 781.28 and 943.35, showing separation of the bi-functionalized products with DP 3, 4 and 5, respectively (note that these are proton adducts). The dashed line shows the time of recording of the MS/MS spectra. B) MS/MS spectra of the three products show a dominant cleavage of the glycosidic bonds followed by multiple water losses (annotated as B-(H2O)2). A cleavage of the reducing-end amine linker bond can also be seen resulting in the following fragments; 543.17, 705.23 and 867.29 for the mother ions 619.25, 781.28 and 943.35, respectively. C) Structures of the three bi-functionalized products, with theoretical masses and the observed fragmentation patterns (using the nomenclature of Domon and Costello) 46 .

    Journal: bioRxiv

    Article Title: Synthesis of Glycoconjugates Utilizing the Regioselectivity of a Lytic Polysaccharide Monooxygenase

    doi: 10.1101/2020.03.13.990838

    Figure Lengend Snippet: PGC-MS analysis of bi-functionalized cello-oligosaccharides. The oxime bi-functionalized oligosaccharides were separated by porous graphitized carbon (PGC) chromatography using an LTQ Velos Pro mass spectrometer (Thermo) for detection. A) Overlay of three extracted ion chromatograms for m/z -ratios 619.25, 781.28 and 943.35, showing separation of the bi-functionalized products with DP 3, 4 and 5, respectively (note that these are proton adducts). The dashed line shows the time of recording of the MS/MS spectra. B) MS/MS spectra of the three products show a dominant cleavage of the glycosidic bonds followed by multiple water losses (annotated as B-(H2O)2). A cleavage of the reducing-end amine linker bond can also be seen resulting in the following fragments; 543.17, 705.23 and 867.29 for the mother ions 619.25, 781.28 and 943.35, respectively. C) Structures of the three bi-functionalized products, with theoretical masses and the observed fragmentation patterns (using the nomenclature of Domon and Costello) 46 .

    Article Snippet: Mass spectrometry of oligosaccharides, direct injection MS, PGC-MS and HILIC-FLD-MS For direct injection, oligosaccharides were analyzed using an LTQ-Velos Pro linear ion trap mass spectrometer (Thermo Scientific, San Jose, CA USA) connected to an Ultimate 3000RS HPLC (Dionex, Sunnyvale, California U.S).

    Techniques: Pyrolysis Gas Chromatography, Chromatography, Mass Spectrometry, Tandem Mass Spectroscopy

    Production of IAA in ipdC -dependent manner by Salmonella enterica sv. Typhimuirum 14028. (A) Detection of IAA by the Salkowski reagent. Results are from replicates from three independent experiments, averages are shown. Error bars are standard deviations. (B) HPLC detection of IAA and tryptophol in spent cultures of the wild type and the ipdC mutant. Hydrophobic fractions of Salmonella culture filtrates were subjected to reverse phase (C 18 ) liquid chromatography, eluted isocratically with acidified water/methanol and eluting substances were detected with a UV/VIS detector set to 230 and 280 nm. (C) Low Resolution Electrospray Ionization LC-MS of the hydrophobic fraction of Salmonella culture filtrates. LTQ LC-MS was carried out using a Grace Vydac 218TP C18 and acetonitrile/water gradient. (D) Expression of the ipdC RIVET reporter in the wild type and Δ ipdC backgrounds was measured in Minimal A medium with and without synthetic IAA or tryptophan in cultures incubated at 22°C for 72 h. Samples were streaked to xylose lysine deoxycholate (XLD) agar with kanamycin at 24 and 72 h. Plates were incubated at 37°C overnight and colonies were patched to LB agar with tetracycline to quantify resolution. Experiments were repeated five times (without technical replications), averages from the five experiments are shown. Error bars are standard deviations.

    Journal: Frontiers in Microbiology

    Article Title: Production of the Plant Hormone Auxin by Salmonella and Its Role in the Interactions with Plants and Animals

    doi: 10.3389/fmicb.2017.02668

    Figure Lengend Snippet: Production of IAA in ipdC -dependent manner by Salmonella enterica sv. Typhimuirum 14028. (A) Detection of IAA by the Salkowski reagent. Results are from replicates from three independent experiments, averages are shown. Error bars are standard deviations. (B) HPLC detection of IAA and tryptophol in spent cultures of the wild type and the ipdC mutant. Hydrophobic fractions of Salmonella culture filtrates were subjected to reverse phase (C 18 ) liquid chromatography, eluted isocratically with acidified water/methanol and eluting substances were detected with a UV/VIS detector set to 230 and 280 nm. (C) Low Resolution Electrospray Ionization LC-MS of the hydrophobic fraction of Salmonella culture filtrates. LTQ LC-MS was carried out using a Grace Vydac 218TP C18 and acetonitrile/water gradient. (D) Expression of the ipdC RIVET reporter in the wild type and Δ ipdC backgrounds was measured in Minimal A medium with and without synthetic IAA or tryptophan in cultures incubated at 22°C for 72 h. Samples were streaked to xylose lysine deoxycholate (XLD) agar with kanamycin at 24 and 72 h. Plates were incubated at 37°C overnight and colonies were patched to LB agar with tetracycline to quantify resolution. Experiments were repeated five times (without technical replications), averages from the five experiments are shown. Error bars are standard deviations.

    Article Snippet: Low Resolution Electrospray Ionization LCMS was carried in a Thermo Scientific LTQ LC-MS instrument using a Grace Vydac 218TP C18 (5 μ, 7.5 cm, 2.1 mm ID) column.

    Techniques: High Performance Liquid Chromatography, Mutagenesis, Liquid Chromatography, Liquid Chromatography with Mass Spectroscopy, Expressing, Incubation

    Influence of the potential applied to the ESI source on retention of UDP-sugars. Chromatogram obtained with a newly installed or regenerated PGC column ( a ); chromatogram obtained after 20-min utilization of the column connected to the ESI source of an Orbitrap mass spectrometer ( b ). Conditions: column regeneration was performed with 80 % acetonitrile in water containing 0.10 % trifluoroacetic acid for 20 min, the sample measurements were done with the gradient program described in the materials and methods section for HPLC-MS; detection, UV, 262 nm; sample. UDP-Gal (100 μmol L −1 ) and UDP-Glc (100 μmol L −1 )

    Journal: Analytical and Bioanalytical Chemistry

    Article Title: Quantitative HPLC-MS analysis of nucleotide sugars in plant cells following off-line SPE sample preparation

    doi: 10.1007/s00216-014-7746-3

    Figure Lengend Snippet: Influence of the potential applied to the ESI source on retention of UDP-sugars. Chromatogram obtained with a newly installed or regenerated PGC column ( a ); chromatogram obtained after 20-min utilization of the column connected to the ESI source of an Orbitrap mass spectrometer ( b ). Conditions: column regeneration was performed with 80 % acetonitrile in water containing 0.10 % trifluoroacetic acid for 20 min, the sample measurements were done with the gradient program described in the materials and methods section for HPLC-MS; detection, UV, 262 nm; sample. UDP-Gal (100 μmol L −1 ) and UDP-Glc (100 μmol L −1 )

    Article Snippet: Fragmentation experiments were performed on a linear ion trap–Orbitrap mass spectrometer (Model LTQ Orbitrap XL, Thermo Fisher Scientific).

    Techniques: Pyrolysis Gas Chromatography, Mass Spectrometry, High Performance Liquid Chromatography, Gas Chromatography