ltq ft ultra mass spectrometer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ltq ft ultra mass spectrometer
    Ltq Ft Ultra Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 151 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltq ft ultra mass spectrometer/product/Thermo Fisher
    Average 92 stars, based on 151 article reviews
    Price from $9.99 to $1999.99
    ltq ft ultra mass spectrometer - by Bioz Stars, 2020-08
    92/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Investigation of the Enteric Pathogenic Potential of Oral Campylobacter concisus Strains Isolated from Patients with Inflammatory Bowel Disease
    Article Snippet: .. Digested peptides were separated by nano-liquid chromatography (LC) using an Ultimate 3000 HPLC and autosampler system (Dionex, Amsterdam, The Netherlands) and then subjected to analysis using a LTQ-FT Ultra mass spectrometer (Thermo Electron, Bremen, Germany). .. All MS/MS spectra were searched against NCBI database using MASCOT (version 2.3).

    Article Title: Mechanism of Intersubunit Ketosynthase-Dehydratase Interaction in Polyketide Synthases
    Article Snippet: .. The HPLC system was coupled to a Thermo Scientific LTQ FT Ultra mass spectrometer equipped with a nanoelectrospray ionization source. .. A 1.7 kV voltage was applied to a coated PicoTip emitter (New Objective).

    Isolation:

    Article Title: Gatekeeping versus Promiscuity in the Early Stages of the Andrimid Biosynthetic Assembly Line
    Article Snippet: .. This mixture was then infused using a Triversa NanoMate (Advion Bio-Sciences) into an LTQ-FT-Ultra mass spectrometer (Thermo Fisher Scientific) operating at 12 T. The 35+ charge state was isolated in the ion trap and detected at 340,000 nominal resolving power at m / z 400. .. AdmF-catalyzed reactions were prepared as above and separated on a 1 mm × 150 mm Jupiter C4 HPLC column (Phenomenex) before infusion into a 12 T LTQ-FT Ultra mass spectrometer.

    Chromatography:

    Article Title: Investigation of the Enteric Pathogenic Potential of Oral Campylobacter concisus Strains Isolated from Patients with Inflammatory Bowel Disease
    Article Snippet: .. Digested peptides were separated by nano-liquid chromatography (LC) using an Ultimate 3000 HPLC and autosampler system (Dionex, Amsterdam, The Netherlands) and then subjected to analysis using a LTQ-FT Ultra mass spectrometer (Thermo Electron, Bremen, Germany). .. All MS/MS spectra were searched against NCBI database using MASCOT (version 2.3).

    Article Title: Surface Proteome of " Mycobacterium avium subsp. hominissuis" during the Early Stages of Macrophage Infection
    Article Snippet: .. Data-dependent liquid chromatography-tandem MS (LC-MS/MS) analyses were performed on an LTQ-FT Ultra mass spectrometer with an IonMax ion source (Thermo, West Palm Beach, FL) coupled to a nanoAcquity Ultra performance LC system (Waters, Milford, MA) equipped with a Michrom peptide CapTrap column and a C18 column (Zorbax 300SB-C18; 250 by 0.3 mm; 5-μm volume; Agilent). ..

    Mass Spectrometry:

    Article Title: Investigation of the Enteric Pathogenic Potential of Oral Campylobacter concisus Strains Isolated from Patients with Inflammatory Bowel Disease
    Article Snippet: .. Digested peptides were separated by nano-liquid chromatography (LC) using an Ultimate 3000 HPLC and autosampler system (Dionex, Amsterdam, The Netherlands) and then subjected to analysis using a LTQ-FT Ultra mass spectrometer (Thermo Electron, Bremen, Germany). .. All MS/MS spectra were searched against NCBI database using MASCOT (version 2.3).

    Article Title: Enduracidin Analogues with Altered Halogenation Patterns Produced by Genetically Engineered Strains of Streptomyces fungicidicus
    Article Snippet: .. The second system is a ThermoFinnigan LTQ-FT Ultra mass spectrometer, equipped with an electrospray ionization source run in positive mode combined with a Waters CapLC system. .. LC-ESI-MS analyses with this instrument were performed with a reversed phase C18 50 × 0.2 mm 5 μm Michrom Magic column, using the mobile phase CH3 CN/H2 O with 0.1% formic acid and a linear gradient from 5% to 60% in 20 min, then to 90% in 10 min, at a flow rate of 4 μL/min.

    Article Title: Mechanism of Intersubunit Ketosynthase-Dehydratase Interaction in Polyketide Synthases
    Article Snippet: .. The HPLC system was coupled to a Thermo Scientific LTQ FT Ultra mass spectrometer equipped with a nanoelectrospray ionization source. .. A 1.7 kV voltage was applied to a coated PicoTip emitter (New Objective).

    Article Title: Proteomic characterization of primary cultured myocytes in a fish model at different myogenesis stages
    Article Snippet: .. The Nanomate was attached to an LTQ-FT Ultra mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) and operated at a spray voltage of 1.7 kV and a delivery pressure of 0.5 psi in positive mode. .. The mass spectrometer was operated in a survey data-dependent acquisition mode.

    Article Title: Gatekeeping versus Promiscuity in the Early Stages of the Andrimid Biosynthetic Assembly Line
    Article Snippet: .. This mixture was then infused using a Triversa NanoMate (Advion Bio-Sciences) into an LTQ-FT-Ultra mass spectrometer (Thermo Fisher Scientific) operating at 12 T. The 35+ charge state was isolated in the ion trap and detected at 340,000 nominal resolving power at m / z 400. .. AdmF-catalyzed reactions were prepared as above and separated on a 1 mm × 150 mm Jupiter C4 HPLC column (Phenomenex) before infusion into a 12 T LTQ-FT Ultra mass spectrometer.

    Article Title: A Strand-Specific RNA-Seq Analysis of the Transcriptome of the Typhoid Bacillus Salmonella Typhi
    Article Snippet: .. The extracted peptides were analyzed with on-line nano LC-MS/MS on an Ultimate 3000 Nano/Capillary LC System (Dionex) coupled to a LTQ FT Ultra mass spectrometer (ThermoElectron) equipped with a nanoelectrospray ion source (NSI). .. Samples were first loaded and desalted on a PepMap C18 trap (0.3 mm id×5 mm, Dionex) then separated on a BEH C18 analytical column (75 µm id×10 cm) over a 30 or 45 or 60 min linear gradient of 4–32% CH3 CN/0.1% FA based on the gel band's size and intensity.

    Article Title: Preliminary identification of differentially expressed tear proteins in keratoconus
    Article Snippet: .. Liquid chromatography and mass spectrometry using linear ion trap quadrupole Fourier transform The digested MF10 fractions were evaluated using a LTQ-FT Ultra mass spectrometer (Thermo Electron, Bremen, Germany). .. Peptides were separated with nano-LC using an Ultimate 3000 HPLC and auto-sampler system (Dionex, Amsterdam, Netherlands).

    Article Title: Surface Proteome of " Mycobacterium avium subsp. hominissuis" during the Early Stages of Macrophage Infection
    Article Snippet: .. Data-dependent liquid chromatography-tandem MS (LC-MS/MS) analyses were performed on an LTQ-FT Ultra mass spectrometer with an IonMax ion source (Thermo, West Palm Beach, FL) coupled to a nanoAcquity Ultra performance LC system (Waters, Milford, MA) equipped with a Michrom peptide CapTrap column and a C18 column (Zorbax 300SB-C18; 250 by 0.3 mm; 5-μm volume; Agilent). ..

    Liquid Chromatography:

    Article Title: Preliminary identification of differentially expressed tear proteins in keratoconus
    Article Snippet: .. Liquid chromatography and mass spectrometry using linear ion trap quadrupole Fourier transform The digested MF10 fractions were evaluated using a LTQ-FT Ultra mass spectrometer (Thermo Electron, Bremen, Germany). .. Peptides were separated with nano-LC using an Ultimate 3000 HPLC and auto-sampler system (Dionex, Amsterdam, Netherlands).

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    Thermo Fisher hybrid linear ion trap ft icr mass spectrometer
    Mass spectrometry characterization of rhamnosylated EF-P. (A) A mass spectrum of His6–EF-P protein, recorded on a <t>7T</t> <t>FT-ICR</t> instrument, from which protein molecular masses were calculated. (B) Lys-C-digested peptide fragmented by ETD maps the additional mass of 146.057 Da on Arg32. The precursor ion, m / z 349.865, is indicated by a dashed line. (C) A proposed fragmentation pattern based on ETD-HCD MS 3 data from the c3 + ion. The neutral losses are colored uniquely to associate the fragment ion with the hypothetical structure. The asterisk indicates a background ion. The precursor ion, m / z 464.246, is indicated by a dashed line.
    Hybrid Linear Ion Trap Ft Icr Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hybrid linear ion trap ft icr mass spectrometer/product/Thermo Fisher
    Average 89 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    hybrid linear ion trap ft icr mass spectrometer - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    89
    Thermo Fisher ltq ft ultra hybrid mass spectrometer
    ESI <t>FT-ICR</t> mass spectra of putative Cancer borealis CHH and its reduction-alkylation product. (a) The accurate mass measurement of putative CHH by 14.5 tesla <t>LTQ</t> FT-ICR MS. The mass difference between calculated and experimental masses indicates the presence
    Ltq Ft Ultra Hybrid Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltq ft ultra hybrid mass spectrometer/product/Thermo Fisher
    Average 89 stars, based on 33 article reviews
    Price from $9.99 to $1999.99
    ltq ft ultra hybrid mass spectrometer - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    85
    Thermo Fisher hybrid linear ion trap ion cyclotron resonance ft ms
    N-terminally acetylated full-length human α-synuclein was the major isoform detected in a ASOTg cortex mitochondrial fraction and in human brain by top-down high-resolution mass spectrometry. A. Total <t>ion</t> chromatogram is shown for the primary separation and the ion-isolation mass spectrum (m/z 967.76) of a candidate full-length α-synuclein of mass 14503.1 Da found in the 37-minute fraction for a ASOTg cortex mitochondrial fraction (500 µg). B. Static nano-electrospray and MSMS displays peaks of various intensities for the average mass spectrum in the same 37-minute fraction (arrow, unmodified human α-synuclein sequenced in panel C). No masses corresponded to known PTMs for human α-synuclein, e,g, nitration, phosphorylation or ubiquitination. C. <t>Hybrid</t> <t>linear</t> <t>ion-trap</t> <t>FT-MS</t> (LTQ-FT) generates high resolution map of product ions formed upon collisionally activated dissociation (CAD) of the m/z 967 precursor, matched at 10 ppm tolerance for confident assignment of the primary amino acid sequence of full-length human α-synuclein from ASOTg mouse (upper panel). Monoisotopic mass of human α-synuclein from ASOTg mouse was 14493.2591 Da (mean of 4 measurements on 4 different ions). A protein of similar mass was recovered from resected human brain and CAD of the m/z 1209 precursor gave a broadly similar product-ion map (lower panel). Monoisotopic mass of human α-synuclein from human brain was 14493.2592 Da (mean of 2 measurements on 2 different ions). The probability that either species analyzed was incorrectly assigned was calculated to be 9.9×10 −31 (ASOTg) and 4.4×10 −43 (human brain) using the ProsightPC algorithm at a tolerance of 10 ppm. Note that the top-down approach yields several product ions in the C-terminal region that was poorly covered in the bottom-up experiment (Fig. 6). The species analyzed is shown with N-terminal acetylation of starting methionine (in red) yielding agreement of measured and calculated masses within 10 ppm.
    Hybrid Linear Ion Trap Ion Cyclotron Resonance Ft Ms, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hybrid linear ion trap ion cyclotron resonance ft ms/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hybrid linear ion trap ion cyclotron resonance ft ms - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    Image Search Results


    Mass spectrometry characterization of rhamnosylated EF-P. (A) A mass spectrum of His6–EF-P protein, recorded on a 7T FT-ICR instrument, from which protein molecular masses were calculated. (B) Lys-C-digested peptide fragmented by ETD maps the additional mass of 146.057 Da on Arg32. The precursor ion, m / z 349.865, is indicated by a dashed line. (C) A proposed fragmentation pattern based on ETD-HCD MS 3 data from the c3 + ion. The neutral losses are colored uniquely to associate the fragment ion with the hypothetical structure. The asterisk indicates a background ion. The precursor ion, m / z 464.246, is indicated by a dashed line.

    Journal: mBio

    Article Title: Cyclic Rhamnosylated Elongation Factor P Establishes Antibiotic Resistance in Pseudomonas aeruginosa

    doi: 10.1128/mBio.00823-15

    Figure Lengend Snippet: Mass spectrometry characterization of rhamnosylated EF-P. (A) A mass spectrum of His6–EF-P protein, recorded on a 7T FT-ICR instrument, from which protein molecular masses were calculated. (B) Lys-C-digested peptide fragmented by ETD maps the additional mass of 146.057 Da on Arg32. The precursor ion, m / z 349.865, is indicated by a dashed line. (C) A proposed fragmentation pattern based on ETD-HCD MS 3 data from the c3 + ion. The neutral losses are colored uniquely to associate the fragment ion with the hypothetical structure. The asterisk indicates a background ion. The precursor ion, m / z 464.246, is indicated by a dashed line.

    Article Snippet: All samples were analyzed using a hybrid linear ion-trap/FT-ICR mass spectrometer (7T, LTQ FT Ultra; Thermo Scientific) operated with a standard (up to m /z 2,000) or extended (up to m /z 4,000) mass range.

    Techniques: Mass Spectrometry

    ESI FT-ICR mass spectra of putative Cancer borealis CHH and its reduction-alkylation product. (a) The accurate mass measurement of putative CHH by 14.5 tesla LTQ FT-ICR MS. The mass difference between calculated and experimental masses indicates the presence

    Journal: Analytical chemistry

    Article Title: Combining Bottom-Up and Top-Down Mass Spectrometric Strategies for De Novo Sequencing of the Crustacean Hyperglycemic Hormone (CHH) from Cancer borealis

    doi: 10.1021/ac801910g

    Figure Lengend Snippet: ESI FT-ICR mass spectra of putative Cancer borealis CHH and its reduction-alkylation product. (a) The accurate mass measurement of putative CHH by 14.5 tesla LTQ FT-ICR MS. The mass difference between calculated and experimental masses indicates the presence

    Article Snippet: Intact protein molecular ions were analyzed with a linear trap/FT-ICR MS (LTQ FT Ultra) hybrid mass spectrometer (Thermo Fisher, Bremen, Germany).

    Techniques: Mass Measurement, Mass Spectrometry

    N-terminally acetylated full-length human α-synuclein was the major isoform detected in a ASOTg cortex mitochondrial fraction and in human brain by top-down high-resolution mass spectrometry. A. Total ion chromatogram is shown for the primary separation and the ion-isolation mass spectrum (m/z 967.76) of a candidate full-length α-synuclein of mass 14503.1 Da found in the 37-minute fraction for a ASOTg cortex mitochondrial fraction (500 µg). B. Static nano-electrospray and MSMS displays peaks of various intensities for the average mass spectrum in the same 37-minute fraction (arrow, unmodified human α-synuclein sequenced in panel C). No masses corresponded to known PTMs for human α-synuclein, e,g, nitration, phosphorylation or ubiquitination. C. Hybrid linear ion-trap FT-MS (LTQ-FT) generates high resolution map of product ions formed upon collisionally activated dissociation (CAD) of the m/z 967 precursor, matched at 10 ppm tolerance for confident assignment of the primary amino acid sequence of full-length human α-synuclein from ASOTg mouse (upper panel). Monoisotopic mass of human α-synuclein from ASOTg mouse was 14493.2591 Da (mean of 4 measurements on 4 different ions). A protein of similar mass was recovered from resected human brain and CAD of the m/z 1209 precursor gave a broadly similar product-ion map (lower panel). Monoisotopic mass of human α-synuclein from human brain was 14493.2592 Da (mean of 2 measurements on 2 different ions). The probability that either species analyzed was incorrectly assigned was calculated to be 9.9×10 −31 (ASOTg) and 4.4×10 −43 (human brain) using the ProsightPC algorithm at a tolerance of 10 ppm. Note that the top-down approach yields several product ions in the C-terminal region that was poorly covered in the bottom-up experiment (Fig. 6). The species analyzed is shown with N-terminal acetylation of starting methionine (in red) yielding agreement of measured and calculated masses within 10 ppm.

    Journal: PLoS ONE

    Article Title: Impairment of Mitochondria in Adult Mouse Brain Overexpressing Predominantly Full-Length, N-Terminally Acetylated Human ?-Synuclein

    doi: 10.1371/journal.pone.0063557

    Figure Lengend Snippet: N-terminally acetylated full-length human α-synuclein was the major isoform detected in a ASOTg cortex mitochondrial fraction and in human brain by top-down high-resolution mass spectrometry. A. Total ion chromatogram is shown for the primary separation and the ion-isolation mass spectrum (m/z 967.76) of a candidate full-length α-synuclein of mass 14503.1 Da found in the 37-minute fraction for a ASOTg cortex mitochondrial fraction (500 µg). B. Static nano-electrospray and MSMS displays peaks of various intensities for the average mass spectrum in the same 37-minute fraction (arrow, unmodified human α-synuclein sequenced in panel C). No masses corresponded to known PTMs for human α-synuclein, e,g, nitration, phosphorylation or ubiquitination. C. Hybrid linear ion-trap FT-MS (LTQ-FT) generates high resolution map of product ions formed upon collisionally activated dissociation (CAD) of the m/z 967 precursor, matched at 10 ppm tolerance for confident assignment of the primary amino acid sequence of full-length human α-synuclein from ASOTg mouse (upper panel). Monoisotopic mass of human α-synuclein from ASOTg mouse was 14493.2591 Da (mean of 4 measurements on 4 different ions). A protein of similar mass was recovered from resected human brain and CAD of the m/z 1209 precursor gave a broadly similar product-ion map (lower panel). Monoisotopic mass of human α-synuclein from human brain was 14493.2592 Da (mean of 2 measurements on 2 different ions). The probability that either species analyzed was incorrectly assigned was calculated to be 9.9×10 −31 (ASOTg) and 4.4×10 −43 (human brain) using the ProsightPC algorithm at a tolerance of 10 ppm. Note that the top-down approach yields several product ions in the C-terminal region that was poorly covered in the bottom-up experiment (Fig. 6). The species analyzed is shown with N-terminal acetylation of starting methionine (in red) yielding agreement of measured and calculated masses within 10 ppm.

    Article Snippet: A hybrid linear ion-trap ion cyclotron resonance FT-MS (7 Tesla LTQ-FT Ultra; Thermo) was used to generate a high-resolution measurement of precursor-ion mass and a map of product ions formed upon collisionally activated dissociation (CAD) of candidate precursor ions corresponding to the full-length α-synuclein of mass 14503.1 Da found in the 37-minute LC-MS+ fraction.

    Techniques: Mass Spectrometry, Isolation, Nitration, Sequencing