ltcc  (Alomone Labs)


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    Structured Review

    Alomone Labs ltcc
    Exercise-induced MnSOD up-regulation attenuates the IR-induced degradation of myocardial Ca 2+ -handling proteins. Western blotting for intact Ca 2+ -handling proteins: <t>LTCC,</t> <t>SERCA2a,</t> PLB, and NCX. Values are mean ± se . * P
    Ltcc, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 80/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltcc/product/Alomone Labs
    Average 80 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    ltcc - by Bioz Stars, 2022-08
    80/100 stars

    Images

    1) Product Images from "Exercise-induced protection against myocardial apoptosis and necrosis: MnSOD, calcium-handling proteins, and calpain"

    Article Title: Exercise-induced protection against myocardial apoptosis and necrosis: MnSOD, calcium-handling proteins, and calpain

    Journal:

    doi: 10.1096/fj.07-102541

    Exercise-induced MnSOD up-regulation attenuates the IR-induced degradation of myocardial Ca 2+ -handling proteins. Western blotting for intact Ca 2+ -handling proteins: LTCC, SERCA2a, PLB, and NCX. Values are mean ± se . * P
    Figure Legend Snippet: Exercise-induced MnSOD up-regulation attenuates the IR-induced degradation of myocardial Ca 2+ -handling proteins. Western blotting for intact Ca 2+ -handling proteins: LTCC, SERCA2a, PLB, and NCX. Values are mean ± se . * P

    Techniques Used: Western Blot

    2) Product Images from "Exercise-induced protection against myocardial apoptosis and necrosis: MnSOD, calcium-handling proteins, and calpain"

    Article Title: Exercise-induced protection against myocardial apoptosis and necrosis: MnSOD, calcium-handling proteins, and calpain

    Journal:

    doi: 10.1096/fj.07-102541

    Exercise-induced MnSOD up-regulation attenuates the IR-induced degradation of myocardial Ca 2+ -handling proteins. Western blotting for intact Ca 2+ -handling proteins: LTCC, SERCA2a, PLB, and NCX. Values are mean ± se . * P
    Figure Legend Snippet: Exercise-induced MnSOD up-regulation attenuates the IR-induced degradation of myocardial Ca 2+ -handling proteins. Western blotting for intact Ca 2+ -handling proteins: LTCC, SERCA2a, PLB, and NCX. Values are mean ± se . * P

    Techniques Used: Western Blot

    3) Product Images from "Severe T-System Remodeling in Pediatric Viral Myocarditis"

    Article Title: Severe T-System Remodeling in Pediatric Viral Myocarditis

    Journal: Frontiers in Cardiovascular Medicine

    doi: 10.3389/fcvm.2020.624776

    Structural integrity of excitation-contraction (EC) coupling junctions before and after VAD therapy of a myocarditis patient. (A,B) Raw confocal images of fixed isolated cardiomyocytes from pre- and post-VAD of the patient presented in Figures 6 , 7 , co-stained for LTCC (red), RyR (green), and JPH2 (blue) and with DAPI (not shown). (C,D) Overlay of binary images for the EC coupling proteins shown in (A,B) , with magnifications of boxed regions. The cell surface, obtained from autofluorescence, is shown white, nuclei are shown white with black asterisk. Co-localizations of LTCC, JPH2, and RyR appear cyan, magenta, yellow, or white (see color legend). (E) Cardiomyocyte JPH2 cluster density (JPH2 density) of AVSD as reference and the Pre- and Post-VAD sample. (F) Fraction of LTCC clusters that were co-localized with both RyR and JPH2, as a measure of intact EC coupling junctions ( n = 10/9 cells for Pre/Post-VAD). * p
    Figure Legend Snippet: Structural integrity of excitation-contraction (EC) coupling junctions before and after VAD therapy of a myocarditis patient. (A,B) Raw confocal images of fixed isolated cardiomyocytes from pre- and post-VAD of the patient presented in Figures 6 , 7 , co-stained for LTCC (red), RyR (green), and JPH2 (blue) and with DAPI (not shown). (C,D) Overlay of binary images for the EC coupling proteins shown in (A,B) , with magnifications of boxed regions. The cell surface, obtained from autofluorescence, is shown white, nuclei are shown white with black asterisk. Co-localizations of LTCC, JPH2, and RyR appear cyan, magenta, yellow, or white (see color legend). (E) Cardiomyocyte JPH2 cluster density (JPH2 density) of AVSD as reference and the Pre- and Post-VAD sample. (F) Fraction of LTCC clusters that were co-localized with both RyR and JPH2, as a measure of intact EC coupling junctions ( n = 10/9 cells for Pre/Post-VAD). * p

    Techniques Used: Isolation, Staining

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    Alomone Labs ltcc
    Exercise-induced MnSOD up-regulation attenuates the IR-induced degradation of myocardial Ca 2+ -handling proteins. Western blotting for intact Ca 2+ -handling proteins: <t>LTCC,</t> <t>SERCA2a,</t> PLB, and NCX. Values are mean ± se . * P
    Ltcc, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltcc/product/Alomone Labs
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ltcc - by Bioz Stars, 2022-08
    80/100 stars
      Buy from Supplier

    95
    Alomone Labs anti ltcc antibody
    Effect of CAL on I Ca , <t>LTCC</t> expression and the systolic Ca transient. A: Representative L-type Ca currents (I Ca ) recorded at 0 mV from Sham (left) and CAL (right) myocytes. B: Mean (± SEM) I Ca density-voltage relations from Sham (circles, n = 50/15) and CAL (squares, n = 41/15) myocytes. C: Representative Western blots of Sham (S) and CAL lysates probed with antibodies against LTCC (top) or <t>GAPDH</t> (bottom). D: Representative whole-cell Ca transients from Sham (grey line) and CAL (black line) myocytes during field stimulation (left); the right panel shows the early rise on an expanded time scale. E: Mean (± SEM) peak ΔF/F 0 , time to peak (TTP), and rate of rise (ΔF/F 0 .ms − 1 ) of the Ca transient in Sham (open bars, n = 10) and CAL (filled bars, n = 16) myocytes. ⁎⁎⁎ p
    Anti Ltcc Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ltcc antibody/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ltcc antibody - by Bioz Stars, 2022-08
    95/100 stars
      Buy from Supplier

    Image Search Results


    Exercise-induced MnSOD up-regulation attenuates the IR-induced degradation of myocardial Ca 2+ -handling proteins. Western blotting for intact Ca 2+ -handling proteins: LTCC, SERCA2a, PLB, and NCX. Values are mean ± se . * P

    Journal:

    Article Title: Exercise-induced protection against myocardial apoptosis and necrosis: MnSOD, calcium-handling proteins, and calpain

    doi: 10.1096/fj.07-102541

    Figure Lengend Snippet: Exercise-induced MnSOD up-regulation attenuates the IR-induced degradation of myocardial Ca 2+ -handling proteins. Western blotting for intact Ca 2+ -handling proteins: LTCC, SERCA2a, PLB, and NCX. Values are mean ± se . * P

    Article Snippet: Sarcoplasmic/endoplasmic reticulum calcium ATPase (SERCA2a), phospholamban (PLB), L-type Ca2+ channels (LTCC), and the Na+ /Ca2+ exchanger (NCX) were isolated by immunoprecipitation using the following primary antibodies: SERCA2a (Affinity Bioreagents, Golden, CO, USA), PLB (Upstate, Lake Placid, NY, USA), LTCC (Alomone Laboratories, Jerusalem, Israel), and NCX (Swant, Bellinzona, Switzerland).

    Techniques: Western Blot

    Structural integrity of excitation-contraction (EC) coupling junctions before and after VAD therapy of a myocarditis patient. (A,B) Raw confocal images of fixed isolated cardiomyocytes from pre- and post-VAD of the patient presented in Figures 6 , 7 , co-stained for LTCC (red), RyR (green), and JPH2 (blue) and with DAPI (not shown). (C,D) Overlay of binary images for the EC coupling proteins shown in (A,B) , with magnifications of boxed regions. The cell surface, obtained from autofluorescence, is shown white, nuclei are shown white with black asterisk. Co-localizations of LTCC, JPH2, and RyR appear cyan, magenta, yellow, or white (see color legend). (E) Cardiomyocyte JPH2 cluster density (JPH2 density) of AVSD as reference and the Pre- and Post-VAD sample. (F) Fraction of LTCC clusters that were co-localized with both RyR and JPH2, as a measure of intact EC coupling junctions ( n = 10/9 cells for Pre/Post-VAD). * p

    Journal: Frontiers in Cardiovascular Medicine

    Article Title: Severe T-System Remodeling in Pediatric Viral Myocarditis

    doi: 10.3389/fcvm.2020.624776

    Figure Lengend Snippet: Structural integrity of excitation-contraction (EC) coupling junctions before and after VAD therapy of a myocarditis patient. (A,B) Raw confocal images of fixed isolated cardiomyocytes from pre- and post-VAD of the patient presented in Figures 6 , 7 , co-stained for LTCC (red), RyR (green), and JPH2 (blue) and with DAPI (not shown). (C,D) Overlay of binary images for the EC coupling proteins shown in (A,B) , with magnifications of boxed regions. The cell surface, obtained from autofluorescence, is shown white, nuclei are shown white with black asterisk. Co-localizations of LTCC, JPH2, and RyR appear cyan, magenta, yellow, or white (see color legend). (E) Cardiomyocyte JPH2 cluster density (JPH2 density) of AVSD as reference and the Pre- and Post-VAD sample. (F) Fraction of LTCC clusters that were co-localized with both RyR and JPH2, as a measure of intact EC coupling junctions ( n = 10/9 cells for Pre/Post-VAD). * p

    Article Snippet: Primary antibodies against JPH2 (Thermo Fisher, 40-5300), LTCC (Alomone, AGP-001), and RyR (Thermo Fisher, MA3 916) were diluted 1:200 in staining solution (phosphate buffered saline, PBS, supplemented with 1% BSA, 5% normal goat serum, 0.25% TritonX-100) and incubated for a minimum of 16h in the dark at 4°C.

    Techniques: Isolation, Staining

    Effect of CAL on I Ca , LTCC expression and the systolic Ca transient. A: Representative L-type Ca currents (I Ca ) recorded at 0 mV from Sham (left) and CAL (right) myocytes. B: Mean (± SEM) I Ca density-voltage relations from Sham (circles, n = 50/15) and CAL (squares, n = 41/15) myocytes. C: Representative Western blots of Sham (S) and CAL lysates probed with antibodies against LTCC (top) or GAPDH (bottom). D: Representative whole-cell Ca transients from Sham (grey line) and CAL (black line) myocytes during field stimulation (left); the right panel shows the early rise on an expanded time scale. E: Mean (± SEM) peak ΔF/F 0 , time to peak (TTP), and rate of rise (ΔF/F 0 .ms − 1 ) of the Ca transient in Sham (open bars, n = 10) and CAL (filled bars, n = 16) myocytes. ⁎⁎⁎ p

    Journal: Journal of Molecular and Cellular Cardiology

    Article Title: Altered distribution of ICa impairs Ca release at the t-tubules of ventricular myocytes from failing hearts

    doi: 10.1016/j.yjmcc.2015.06.012

    Figure Lengend Snippet: Effect of CAL on I Ca , LTCC expression and the systolic Ca transient. A: Representative L-type Ca currents (I Ca ) recorded at 0 mV from Sham (left) and CAL (right) myocytes. B: Mean (± SEM) I Ca density-voltage relations from Sham (circles, n = 50/15) and CAL (squares, n = 41/15) myocytes. C: Representative Western blots of Sham (S) and CAL lysates probed with antibodies against LTCC (top) or GAPDH (bottom). D: Representative whole-cell Ca transients from Sham (grey line) and CAL (black line) myocytes during field stimulation (left); the right panel shows the early rise on an expanded time scale. E: Mean (± SEM) peak ΔF/F 0 , time to peak (TTP), and rate of rise (ΔF/F 0 .ms − 1 ) of the Ca transient in Sham (open bars, n = 10) and CAL (filled bars, n = 16) myocytes. ⁎⁎⁎ p

    Article Snippet: The blot was probed with anti-LTCC antibody (ACC-003; Alomone, Israel) or anti-GAPDH (G9545; Sigma) and protein bands visualized using relevant peroxidase-conjugated secondary antibodies, chemiluminescence, and autoradiography.

    Techniques: Expressing, Western Blot