Journal: Disease Markers

Article Title: Fluvastatin Upregulates the α 1C Subunit of CaV1.2 Channel Expression in Vascular Smooth Muscle Cells via RhoA and ERK/p38 MAPK Pathways

doi: 10.1155/2014/237067

Figure Lengend Snippet: Effects of Flu on the levels of LTCC α 1C protein determined by western blot analysis. PDGF time-dependently inhibited LTCC α 1C expression, which could be prevented by fluvastatin. (a) Dose-effect response of PDGF stimulation on the LTCC α 1C expression in VSMCs. (b) Time-effect response of Flu on the LTCC α 1C expression incubated by PDGF. β-actin served as the loading control. Data was represented as means ± SE of 3 separate experiments conducted in triplicate. * P < 0.05 versus blank control and # P < 0.05 versus cells incubation with PDGF. LTCC α 1C : L-type calcium channel α 1C subunit; Flu: fluvastatin; and PDGF: platelet derived growth factor.

Article Snippet: Primary antibodies were used at the indicated dilutions as follows: LTCC α 1C , 1 : 200 (Alomone); β -actin, 1 : 1000; anti-p-p38 MAPK, 1 : 1000; anti-total-p38 MAPK, 1 : 1000; anti-p-JNK, 1 : 1000; anti-total-JNK 1 : 1000; anti-p-ERK1/2 (1 : 1000); and anti-total-ERK1/2 (1 : 1000); all were from Cell Signalling Technology (Danvers, MA).

Techniques: Western Blot, Expressing, Incubation, Derivative Assay

Journal: Disease Markers

Article Title: Fluvastatin Upregulates the α 1C Subunit of CaV1.2 Channel Expression in Vascular Smooth Muscle Cells via RhoA and ERK/p38 MAPK Pathways

doi: 10.1155/2014/237067

Figure Lengend Snippet: Effects of MAPK or ROCK inhibition on LTCC α 1C expression after PDGF stimulation in VSMCs. LTCC α 1C protein expression was evaluated by western blot analysis. Data was described as means ± SEM from three experiments performed in triplicate. * P < 0.05 versus blank control and # P < 0.05 versus PDGF stimulated cells. MAPK: mitogen activated protein kinase; ROCK: Rho associated protein kinase; LTCC α 1C : L-type calcium channel α 1C subunit; Flu: fluvastatin; and PDGF: platelet derived growth factor.

Article Snippet: Primary antibodies were used at the indicated dilutions as follows: LTCC α 1C , 1 : 200 (Alomone); β -actin, 1 : 1000; anti-p-p38 MAPK, 1 : 1000; anti-total-p38 MAPK, 1 : 1000; anti-p-JNK, 1 : 1000; anti-total-JNK 1 : 1000; anti-p-ERK1/2 (1 : 1000); and anti-total-ERK1/2 (1 : 1000); all were from Cell Signalling Technology (Danvers, MA).

Techniques: Inhibition, Expressing, Western Blot, Derivative Assay