Journal: Annals of Translational Medicine
Article Title: Inhibition of laser induced rats choroidal neovascularization by intravitreous injection of sEphB4-HSA
Figure Lengend Snippet: Effects of the sEphB4 injection on fluorescein leakage and CNV (choroidal neovascularization) lesion in rat. Fluorescein angiogram (FA) was performed 14 days after laser photocoagulation and intravitreal injection of the sEphB4. (A) Representative FA images in laser-induced rat (B) FA score: sEphB4 injection resulted in a dose dependent inhibition of fluorescein leakage CNV (20 FA images were pooled in each group). (C) Representative CNV lesions from PBS or different concentrations of sEphB4-HSA injection measured in FITC-labeled isolectin-B4 stained flat retinal mounts on day 14. (D) After being stained with isolectin B4, retinal flatmounts were visualized with a laser scanning confocal microscope Z stack images of CNV lesion were taken. The image stacks were rendered in 3D using velocity imaging software (Improvision Inc., Waltham, USA) and processed to digitally extract the fluorescent lesion volume. CNV volume was measured in micrometers cubed (14 images/each group), A dose dependent significant reduction of CNV volume was seen in EphB4-HSA treated rats compared with PBS (phosphate-buffered saline) injection.
Article Snippet: The sections were cover-slipped (Vectashield medium; Vector Laboratories), and the lectin labeling was examined with the 20× objective of a laser scanning confocal microscope (LSM; model 510; Carl Zeiss Meditec, Dublin, CA).
Techniques: Injection, Inhibition, Labeling, Staining, Microscopy, Imaging, Software