human ls174t cell line  (ATCC)


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    ATCC human ls174t cell line
    Human Ls174t Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human ls174t cell line  (ATCC)


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    ATCC human ls174t cell line
    Human Ls174t Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    colorectal adenocarcinoma cell line ls174t  (ATCC)


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    ATCC colorectal adenocarcinoma cell line ls174t
    Interaction between mHsp70‐expressing cancer cells and IL‐2/TKD‐St CD94 high and CD94 Low NK cells via ligand‐receptor binding processes. A) Representative fluorescence images of <t>LS174T</t> and UD‐5 cells expressing high densities of membrane Hsp70 (mHsp70) using FITC‐cmHsp70.1 mAb (Green). Nuclei and F‐actin skeleton were stained with DAPI (Blue) and phalloidin (Red), respectively. The scale bars are 15 and 25 µm for LS174T and UD‐5, respectively. B) Representative flow cytometric measurement of mHsp70 expression using FITC‐cmHsp70.1 mAb (Green histograms). Isotype‐matched (Mouse FITC‐IgG1) mAb was used as negative control (Gray histograms). Data are shown as positivity percentage and MFI fold change. The fold change was assessed by dividing the expression value of the FITC‐cmHsp70.1‐treated cells by the values generated for isotype‐matched cells. C) Cancer cell apoptosis detected by Annexin V/PI assay. Prior to measurement, target cells (2 × 10 5 cells well −1 ) were incubated with IL‐2/TKD‐St NK cells at a ratio of 1:1 for 8 h at 37 °C ( n ≥ 3 independent donors, triplicate mean ± SD). D) The positivity ratio of IL‐2/TKD‐St to Unst NK cells for intracellular perforin and GrB contents after an 8‐h co‐incubation with cancer cells. E) Multiplex cytokine/chemokine levels quantified by flow cytometry in the supernatant media. For this, the cancer cells were co‐cultured with IL‐2/TKD‐St NK cells for 8 h. Levels of significance are shown as * p ≤ 0.05, ** p ≤ 0.01.
    Colorectal Adenocarcinoma Cell Line Ls174t, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Crosstalk Between NK Cell Receptors and Tumor Membrane Hsp70‐Derived Peptide: A Combined Computational and Experimental Study"

    Article Title: Crosstalk Between NK Cell Receptors and Tumor Membrane Hsp70‐Derived Peptide: A Combined Computational and Experimental Study

    Journal: Advanced Science

    doi: 10.1002/advs.202305998

    Interaction between mHsp70‐expressing cancer cells and IL‐2/TKD‐St CD94 high and CD94 Low NK cells via ligand‐receptor binding processes. A) Representative fluorescence images of LS174T and UD‐5 cells expressing high densities of membrane Hsp70 (mHsp70) using FITC‐cmHsp70.1 mAb (Green). Nuclei and F‐actin skeleton were stained with DAPI (Blue) and phalloidin (Red), respectively. The scale bars are 15 and 25 µm for LS174T and UD‐5, respectively. B) Representative flow cytometric measurement of mHsp70 expression using FITC‐cmHsp70.1 mAb (Green histograms). Isotype‐matched (Mouse FITC‐IgG1) mAb was used as negative control (Gray histograms). Data are shown as positivity percentage and MFI fold change. The fold change was assessed by dividing the expression value of the FITC‐cmHsp70.1‐treated cells by the values generated for isotype‐matched cells. C) Cancer cell apoptosis detected by Annexin V/PI assay. Prior to measurement, target cells (2 × 10 5 cells well −1 ) were incubated with IL‐2/TKD‐St NK cells at a ratio of 1:1 for 8 h at 37 °C ( n ≥ 3 independent donors, triplicate mean ± SD). D) The positivity ratio of IL‐2/TKD‐St to Unst NK cells for intracellular perforin and GrB contents after an 8‐h co‐incubation with cancer cells. E) Multiplex cytokine/chemokine levels quantified by flow cytometry in the supernatant media. For this, the cancer cells were co‐cultured with IL‐2/TKD‐St NK cells for 8 h. Levels of significance are shown as * p ≤ 0.05, ** p ≤ 0.01.
    Figure Legend Snippet: Interaction between mHsp70‐expressing cancer cells and IL‐2/TKD‐St CD94 high and CD94 Low NK cells via ligand‐receptor binding processes. A) Representative fluorescence images of LS174T and UD‐5 cells expressing high densities of membrane Hsp70 (mHsp70) using FITC‐cmHsp70.1 mAb (Green). Nuclei and F‐actin skeleton were stained with DAPI (Blue) and phalloidin (Red), respectively. The scale bars are 15 and 25 µm for LS174T and UD‐5, respectively. B) Representative flow cytometric measurement of mHsp70 expression using FITC‐cmHsp70.1 mAb (Green histograms). Isotype‐matched (Mouse FITC‐IgG1) mAb was used as negative control (Gray histograms). Data are shown as positivity percentage and MFI fold change. The fold change was assessed by dividing the expression value of the FITC‐cmHsp70.1‐treated cells by the values generated for isotype‐matched cells. C) Cancer cell apoptosis detected by Annexin V/PI assay. Prior to measurement, target cells (2 × 10 5 cells well −1 ) were incubated with IL‐2/TKD‐St NK cells at a ratio of 1:1 for 8 h at 37 °C ( n ≥ 3 independent donors, triplicate mean ± SD). D) The positivity ratio of IL‐2/TKD‐St to Unst NK cells for intracellular perforin and GrB contents after an 8‐h co‐incubation with cancer cells. E) Multiplex cytokine/chemokine levels quantified by flow cytometry in the supernatant media. For this, the cancer cells were co‐cultured with IL‐2/TKD‐St NK cells for 8 h. Levels of significance are shown as * p ≤ 0.05, ** p ≤ 0.01.

    Techniques Used: Expressing, Binding Assay, Fluorescence, Membrane, Staining, Negative Control, Generated, Incubation, Multiplex Assay, Flow Cytometry, Cell Culture

    ls 174t cell lines  (ATCC)


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    ATCC ls 174t cell lines
    Ls 174t Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ls 174t cell lines  (ATCC)


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    ATCC ls 174t cell lines
    Ls 174t Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ls174t colon cancer cells  (ATCC)


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    ATCC ls174t colon cancer cells
    ( A ) Representative immunofluorescence of <t>LS174T</t> cells treated with DMSO, 10 μM XAV939 or 10 μM Erlotinib as indicated for 24 h. Cells were stained with β-Catenin antibody followed by Alexa488 secondary antibody for visualization and counterstained with DAPI Scale bar = 10 μm. Staining controls are showing in . ( B ) ImageJ quantification of the integrated density of the β-Catenin signal in the nucleus. * ** p = 0.0003; * ** * p ≤ 0.0001, compared to DMSO treatment. ( C ) Representative western blot of LS174T colorectal cancer cell lysates treated with DMSO or 10 μM Erlotinib for 24 h. Blots were probed with the indicated antibodies and β actin was used as a loading control. Uncropped blots and replicates are shown in .
    Ls174t Colon Cancer Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Zebrafish drug screening identifies Erlotinib as an inhibitor of Wnt/β-catenin signaling and self-renewal in T-cell acute lymphoblastic leukemia"

    Article Title: Zebrafish drug screening identifies Erlotinib as an inhibitor of Wnt/β-catenin signaling and self-renewal in T-cell acute lymphoblastic leukemia

    Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

    doi: 10.1016/j.biopha.2023.116013

    ( A ) Representative immunofluorescence of LS174T cells treated with DMSO, 10 μM XAV939 or 10 μM Erlotinib as indicated for 24 h. Cells were stained with β-Catenin antibody followed by Alexa488 secondary antibody for visualization and counterstained with DAPI Scale bar = 10 μm. Staining controls are showing in . ( B ) ImageJ quantification of the integrated density of the β-Catenin signal in the nucleus. * ** p = 0.0003; * ** * p ≤ 0.0001, compared to DMSO treatment. ( C ) Representative western blot of LS174T colorectal cancer cell lysates treated with DMSO or 10 μM Erlotinib for 24 h. Blots were probed with the indicated antibodies and β actin was used as a loading control. Uncropped blots and replicates are shown in .
    Figure Legend Snippet: ( A ) Representative immunofluorescence of LS174T cells treated with DMSO, 10 μM XAV939 or 10 μM Erlotinib as indicated for 24 h. Cells were stained with β-Catenin antibody followed by Alexa488 secondary antibody for visualization and counterstained with DAPI Scale bar = 10 μm. Staining controls are showing in . ( B ) ImageJ quantification of the integrated density of the β-Catenin signal in the nucleus. * ** p = 0.0003; * ** * p ≤ 0.0001, compared to DMSO treatment. ( C ) Representative western blot of LS174T colorectal cancer cell lysates treated with DMSO or 10 μM Erlotinib for 24 h. Blots were probed with the indicated antibodies and β actin was used as a loading control. Uncropped blots and replicates are shown in .

    Techniques Used: Immunofluorescence, Staining, Western Blot

    ls174t cells  (ATCC)


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    ATCC ls174t cells
    Ls174t Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ls174t human colorectal adenocarcinoma cell line  (ATCC)


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    ATCC ls174t human colorectal adenocarcinoma cell line
    Ls174t Human Colorectal Adenocarcinoma Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ls174t human colorectal adenocarcinoma cell line  (ATCC)


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    ATCC ls174t human colorectal adenocarcinoma cell line
    Diclofenac inhibits cell viability and c-MYC expression in <t>LS174T,</t> LoVo, A549 and MDA-MB-231 cancer cells. ( A-D ) Toxicity assay of LS174T (A), LoVo (B), A549 (C) and MDA-MB-231 (D) cancers cells treated with different diclofenac concentrations (0, 0.1, 0.2 and 0.4 mM) for 24 and 48 h. Two way ANOVA was used to evaluate significant differences (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001). ( E-G ) Representative immunoblot showing the expression of c-MYC 48 h after diclofenac treatment in LS174T (E), LoVo (F) and A549 (G) cancer cells. Quantification of the c-MYC signals of at least 3 independent experiments are shown in the bar charts above (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001)
    Ls174t Human Colorectal Adenocarcinoma Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "The radiation- and chemo-sensitizing capacity of diclofenac can be predicted by a decreased lactate metabolism and stress response"

    Article Title: The radiation- and chemo-sensitizing capacity of diclofenac can be predicted by a decreased lactate metabolism and stress response

    Journal: Radiation Oncology (London, England)

    doi: 10.1186/s13014-024-02399-5

    Diclofenac inhibits cell viability and c-MYC expression in LS174T, LoVo, A549 and MDA-MB-231 cancer cells. ( A-D ) Toxicity assay of LS174T (A), LoVo (B), A549 (C) and MDA-MB-231 (D) cancers cells treated with different diclofenac concentrations (0, 0.1, 0.2 and 0.4 mM) for 24 and 48 h. Two way ANOVA was used to evaluate significant differences (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001). ( E-G ) Representative immunoblot showing the expression of c-MYC 48 h after diclofenac treatment in LS174T (E), LoVo (F) and A549 (G) cancer cells. Quantification of the c-MYC signals of at least 3 independent experiments are shown in the bar charts above (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001)
    Figure Legend Snippet: Diclofenac inhibits cell viability and c-MYC expression in LS174T, LoVo, A549 and MDA-MB-231 cancer cells. ( A-D ) Toxicity assay of LS174T (A), LoVo (B), A549 (C) and MDA-MB-231 (D) cancers cells treated with different diclofenac concentrations (0, 0.1, 0.2 and 0.4 mM) for 24 and 48 h. Two way ANOVA was used to evaluate significant differences (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001). ( E-G ) Representative immunoblot showing the expression of c-MYC 48 h after diclofenac treatment in LS174T (E), LoVo (F) and A549 (G) cancer cells. Quantification of the c-MYC signals of at least 3 independent experiments are shown in the bar charts above (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001)

    Techniques Used: Expressing, Western Blot

    Effects of diclofenac on membrane Hsp70 expression. Plasma membrane Hsp70 expression on untreated and diclofenac treated (0.1, 0.2 mM for 48 h) LS174T ( A ), LoVo ( B ), A549 ( C ) and MDA-MB-231 ( D ) cancer cells. Data present the proportion of positively stained cells of at least 3 independent experiments. ( E ) Representative gating strategy to determine membrane Hsp70 expression on viable tumor cells. Left, side scatter (SSC)/forward scatter (FSC) dot plot histogram to identify the tumor cell population based on size (FSC) and granularity (SSC); middle, gating of Propidium Iodide (PI) negative, viable tumor cells (96.0%); right, overlay of two histograms representing membrane Hsp70 positive tumor cells (43.1%) using cmHsp70.1-FITC monoclonal antibody (mAb, gray) and the negative control (white histogram) using an isotype-matched mAb (anti-mouse IgG1-FITC). The one way ANOVA test was used to evaluate significant differences (* p ≤ 0.05, ** p ≤ 0.01)
    Figure Legend Snippet: Effects of diclofenac on membrane Hsp70 expression. Plasma membrane Hsp70 expression on untreated and diclofenac treated (0.1, 0.2 mM for 48 h) LS174T ( A ), LoVo ( B ), A549 ( C ) and MDA-MB-231 ( D ) cancer cells. Data present the proportion of positively stained cells of at least 3 independent experiments. ( E ) Representative gating strategy to determine membrane Hsp70 expression on viable tumor cells. Left, side scatter (SSC)/forward scatter (FSC) dot plot histogram to identify the tumor cell population based on size (FSC) and granularity (SSC); middle, gating of Propidium Iodide (PI) negative, viable tumor cells (96.0%); right, overlay of two histograms representing membrane Hsp70 positive tumor cells (43.1%) using cmHsp70.1-FITC monoclonal antibody (mAb, gray) and the negative control (white histogram) using an isotype-matched mAb (anti-mouse IgG1-FITC). The one way ANOVA test was used to evaluate significant differences (* p ≤ 0.05, ** p ≤ 0.01)

    Techniques Used: Membrane, Expressing, Staining, Negative Control

    Colony forming assay for LS174T, LoVo, A549 and MDA-MB-231 cells. LS174T ( A ), LoVo ( B ), A549 ( C ) and MDA-MB-231 ( D ) cancer cells were treated with 0.1 mM diclofenac (Dic) for 48 h and then were irradiated (0–6 Gy). The cells were allowed to form colonies in drug-free medium. Two way ANOVA was used to evaluate significant differences (* p ≤ 0.05***, p ≤ 0.001). LS174T ( E ), LoVo ( F ), A549 ( G ) and MDA-MB-231 ( H ) were kept untreated or were treated with 0.1 mM diclofenac, 1 or 5 µM 5-fluorouracil (5-FU) or with diclofenac and 5-FU. After 48 h the medium was changed and cells were allowed to form colonies. The colony forming assay represents the results of at least 3 independent experiments. The one way ANOVA test was used to evaluate significant differences (** p ≤ 0.01, *** p ≤ 0.001)
    Figure Legend Snippet: Colony forming assay for LS174T, LoVo, A549 and MDA-MB-231 cells. LS174T ( A ), LoVo ( B ), A549 ( C ) and MDA-MB-231 ( D ) cancer cells were treated with 0.1 mM diclofenac (Dic) for 48 h and then were irradiated (0–6 Gy). The cells were allowed to form colonies in drug-free medium. Two way ANOVA was used to evaluate significant differences (* p ≤ 0.05***, p ≤ 0.001). LS174T ( E ), LoVo ( F ), A549 ( G ) and MDA-MB-231 ( H ) were kept untreated or were treated with 0.1 mM diclofenac, 1 or 5 µM 5-fluorouracil (5-FU) or with diclofenac and 5-FU. After 48 h the medium was changed and cells were allowed to form colonies. The colony forming assay represents the results of at least 3 independent experiments. The one way ANOVA test was used to evaluate significant differences (** p ≤ 0.01, *** p ≤ 0.001)

    Techniques Used: Irradiation

    Diclofenac enhances the effect of radiotherapy in a LS174T xenograft mouse model. ( A ) Treatment schedule. C57/BL6J mice were exposed to a whole-body irradiation (3 Gy) to immunocompromise the animals. 24 h after irradiation, LS174T cells were injected subcutaneously (s.c.) into the right shoulder region. On day 7 after the tumor cell injection mice with identical tumor sizes were randomly divided into the following four groups each with 4 animals: control (ctrl, n = 4), diclofenac (Dic, n = 4), radiation (IR, n = 4) and diclofenac-radiation (Dic + IR, n = 4). In the diclofenac and diclofenac-radiation groups, mice received intraperitoneal injections of diclofenac (40 mg/kg) on days 7, 9 and 11. Then the diclofenac-radiation and radiation groups were locally irradiated with a single dose of 6 Gy on day 11. On day 16 all animals were euthanized and the tumors and organs were excised for analysis. ( B ) Tumor volume was measured regularly every 3 days with a digital caliper. Kruskal Wallis Test was used to evaluate significant differences (* p ≤ 0.05). ( C ) After euthanization the weights of the mice and the excised tumors were determined. Kruskal Wallis Test was used to evaluate significant differences (* p ≤ 0.05)
    Figure Legend Snippet: Diclofenac enhances the effect of radiotherapy in a LS174T xenograft mouse model. ( A ) Treatment schedule. C57/BL6J mice were exposed to a whole-body irradiation (3 Gy) to immunocompromise the animals. 24 h after irradiation, LS174T cells were injected subcutaneously (s.c.) into the right shoulder region. On day 7 after the tumor cell injection mice with identical tumor sizes were randomly divided into the following four groups each with 4 animals: control (ctrl, n = 4), diclofenac (Dic, n = 4), radiation (IR, n = 4) and diclofenac-radiation (Dic + IR, n = 4). In the diclofenac and diclofenac-radiation groups, mice received intraperitoneal injections of diclofenac (40 mg/kg) on days 7, 9 and 11. Then the diclofenac-radiation and radiation groups were locally irradiated with a single dose of 6 Gy on day 11. On day 16 all animals were euthanized and the tumors and organs were excised for analysis. ( B ) Tumor volume was measured regularly every 3 days with a digital caliper. Kruskal Wallis Test was used to evaluate significant differences (* p ≤ 0.05). ( C ) After euthanization the weights of the mice and the excised tumors were determined. Kruskal Wallis Test was used to evaluate significant differences (* p ≤ 0.05)

    Techniques Used: Irradiation, Injection

    ls 174t cell lines  (ATCC)


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    ATCC ls 174t cell lines
    In vitro study of the expression of GR in colorectal cancer cell lines that represent a different type of colorectal cancer. The analysis revealed that the LS <t>174T</t> cell line had the highest level of GR protein expression. A similar level of GR expression was observed in the SW1116 cell line, HCA-2 cell line and CCD 841 CoN ( A ). Significant differences in the levels of GR protein expression were observed between the HCA-2 cells and the LS 174T, between CCD 841 CoN and <t>LS174T,</t> and between SW 1116 and LS 174T. Statistical significance was determined using an independent-sample t -test. Data are shown as mean ± SD of values of three measurements in each group. In all figures, p -value of statistical significance is ** p < 0.01 ( B ).
    Ls 174t Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Glutathione Reductase Expression and Its Prognostic Significance in Colon Cancer"

    Article Title: Glutathione Reductase Expression and Its Prognostic Significance in Colon Cancer

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms25021097

    In vitro study of the expression of GR in colorectal cancer cell lines that represent a different type of colorectal cancer. The analysis revealed that the LS 174T cell line had the highest level of GR protein expression. A similar level of GR expression was observed in the SW1116 cell line, HCA-2 cell line and CCD 841 CoN ( A ). Significant differences in the levels of GR protein expression were observed between the HCA-2 cells and the LS 174T, between CCD 841 CoN and LS174T, and between SW 1116 and LS 174T. Statistical significance was determined using an independent-sample t -test. Data are shown as mean ± SD of values of three measurements in each group. In all figures, p -value of statistical significance is ** p < 0.01 ( B ).
    Figure Legend Snippet: In vitro study of the expression of GR in colorectal cancer cell lines that represent a different type of colorectal cancer. The analysis revealed that the LS 174T cell line had the highest level of GR protein expression. A similar level of GR expression was observed in the SW1116 cell line, HCA-2 cell line and CCD 841 CoN ( A ). Significant differences in the levels of GR protein expression were observed between the HCA-2 cells and the LS 174T, between CCD 841 CoN and LS174T, and between SW 1116 and LS 174T. Statistical significance was determined using an independent-sample t -test. Data are shown as mean ± SD of values of three measurements in each group. In all figures, p -value of statistical significance is ** p < 0.01 ( B ).

    Techniques Used: In Vitro, Expressing

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    ATCC human ls174t cell line
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    ATCC colorectal adenocarcinoma cell line ls174t
    Interaction between mHsp70‐expressing cancer cells and IL‐2/TKD‐St CD94 high and CD94 Low NK cells via ligand‐receptor binding processes. A) Representative fluorescence images of <t>LS174T</t> and UD‐5 cells expressing high densities of membrane Hsp70 (mHsp70) using FITC‐cmHsp70.1 mAb (Green). Nuclei and F‐actin skeleton were stained with DAPI (Blue) and phalloidin (Red), respectively. The scale bars are 15 and 25 µm for LS174T and UD‐5, respectively. B) Representative flow cytometric measurement of mHsp70 expression using FITC‐cmHsp70.1 mAb (Green histograms). Isotype‐matched (Mouse FITC‐IgG1) mAb was used as negative control (Gray histograms). Data are shown as positivity percentage and MFI fold change. The fold change was assessed by dividing the expression value of the FITC‐cmHsp70.1‐treated cells by the values generated for isotype‐matched cells. C) Cancer cell apoptosis detected by Annexin V/PI assay. Prior to measurement, target cells (2 × 10 5 cells well −1 ) were incubated with IL‐2/TKD‐St NK cells at a ratio of 1:1 for 8 h at 37 °C ( n ≥ 3 independent donors, triplicate mean ± SD). D) The positivity ratio of IL‐2/TKD‐St to Unst NK cells for intracellular perforin and GrB contents after an 8‐h co‐incubation with cancer cells. E) Multiplex cytokine/chemokine levels quantified by flow cytometry in the supernatant media. For this, the cancer cells were co‐cultured with IL‐2/TKD‐St NK cells for 8 h. Levels of significance are shown as * p ≤ 0.05, ** p ≤ 0.01.
    Colorectal Adenocarcinoma Cell Line Ls174t, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC ls 174t cell lines
    Interaction between mHsp70‐expressing cancer cells and IL‐2/TKD‐St CD94 high and CD94 Low NK cells via ligand‐receptor binding processes. A) Representative fluorescence images of <t>LS174T</t> and UD‐5 cells expressing high densities of membrane Hsp70 (mHsp70) using FITC‐cmHsp70.1 mAb (Green). Nuclei and F‐actin skeleton were stained with DAPI (Blue) and phalloidin (Red), respectively. The scale bars are 15 and 25 µm for LS174T and UD‐5, respectively. B) Representative flow cytometric measurement of mHsp70 expression using FITC‐cmHsp70.1 mAb (Green histograms). Isotype‐matched (Mouse FITC‐IgG1) mAb was used as negative control (Gray histograms). Data are shown as positivity percentage and MFI fold change. The fold change was assessed by dividing the expression value of the FITC‐cmHsp70.1‐treated cells by the values generated for isotype‐matched cells. C) Cancer cell apoptosis detected by Annexin V/PI assay. Prior to measurement, target cells (2 × 10 5 cells well −1 ) were incubated with IL‐2/TKD‐St NK cells at a ratio of 1:1 for 8 h at 37 °C ( n ≥ 3 independent donors, triplicate mean ± SD). D) The positivity ratio of IL‐2/TKD‐St to Unst NK cells for intracellular perforin and GrB contents after an 8‐h co‐incubation with cancer cells. E) Multiplex cytokine/chemokine levels quantified by flow cytometry in the supernatant media. For this, the cancer cells were co‐cultured with IL‐2/TKD‐St NK cells for 8 h. Levels of significance are shown as * p ≤ 0.05, ** p ≤ 0.01.
    Ls 174t Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ls 174t cell lines/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    ATCC ls174t colon cancer cells
    ( A ) Representative immunofluorescence of <t>LS174T</t> cells treated with DMSO, 10 μM XAV939 or 10 μM Erlotinib as indicated for 24 h. Cells were stained with β-Catenin antibody followed by Alexa488 secondary antibody for visualization and counterstained with DAPI Scale bar = 10 μm. Staining controls are showing in . ( B ) ImageJ quantification of the integrated density of the β-Catenin signal in the nucleus. * ** p = 0.0003; * ** * p ≤ 0.0001, compared to DMSO treatment. ( C ) Representative western blot of LS174T colorectal cancer cell lysates treated with DMSO or 10 μM Erlotinib for 24 h. Blots were probed with the indicated antibodies and β actin was used as a loading control. Uncropped blots and replicates are shown in .
    Ls174t Colon Cancer Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ls174t colon cancer cells/product/ATCC
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    ATCC ls174t cells
    ( A ) Representative immunofluorescence of <t>LS174T</t> cells treated with DMSO, 10 μM XAV939 or 10 μM Erlotinib as indicated for 24 h. Cells were stained with β-Catenin antibody followed by Alexa488 secondary antibody for visualization and counterstained with DAPI Scale bar = 10 μm. Staining controls are showing in . ( B ) ImageJ quantification of the integrated density of the β-Catenin signal in the nucleus. * ** p = 0.0003; * ** * p ≤ 0.0001, compared to DMSO treatment. ( C ) Representative western blot of LS174T colorectal cancer cell lysates treated with DMSO or 10 μM Erlotinib for 24 h. Blots were probed with the indicated antibodies and β actin was used as a loading control. Uncropped blots and replicates are shown in .
    Ls174t Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ls174t cells/product/ATCC
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    ls174t cells - by Bioz Stars, 2024-04
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    ATCC ls174t human colorectal adenocarcinoma cell line
    ( A ) Representative immunofluorescence of <t>LS174T</t> cells treated with DMSO, 10 μM XAV939 or 10 μM Erlotinib as indicated for 24 h. Cells were stained with β-Catenin antibody followed by Alexa488 secondary antibody for visualization and counterstained with DAPI Scale bar = 10 μm. Staining controls are showing in . ( B ) ImageJ quantification of the integrated density of the β-Catenin signal in the nucleus. * ** p = 0.0003; * ** * p ≤ 0.0001, compared to DMSO treatment. ( C ) Representative western blot of LS174T colorectal cancer cell lysates treated with DMSO or 10 μM Erlotinib for 24 h. Blots were probed with the indicated antibodies and β actin was used as a loading control. Uncropped blots and replicates are shown in .
    Ls174t Human Colorectal Adenocarcinoma Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ls174t human colorectal adenocarcinoma cell line/product/ATCC
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    Image Search Results


    Interaction between mHsp70‐expressing cancer cells and IL‐2/TKD‐St CD94 high and CD94 Low NK cells via ligand‐receptor binding processes. A) Representative fluorescence images of LS174T and UD‐5 cells expressing high densities of membrane Hsp70 (mHsp70) using FITC‐cmHsp70.1 mAb (Green). Nuclei and F‐actin skeleton were stained with DAPI (Blue) and phalloidin (Red), respectively. The scale bars are 15 and 25 µm for LS174T and UD‐5, respectively. B) Representative flow cytometric measurement of mHsp70 expression using FITC‐cmHsp70.1 mAb (Green histograms). Isotype‐matched (Mouse FITC‐IgG1) mAb was used as negative control (Gray histograms). Data are shown as positivity percentage and MFI fold change. The fold change was assessed by dividing the expression value of the FITC‐cmHsp70.1‐treated cells by the values generated for isotype‐matched cells. C) Cancer cell apoptosis detected by Annexin V/PI assay. Prior to measurement, target cells (2 × 10 5 cells well −1 ) were incubated with IL‐2/TKD‐St NK cells at a ratio of 1:1 for 8 h at 37 °C ( n ≥ 3 independent donors, triplicate mean ± SD). D) The positivity ratio of IL‐2/TKD‐St to Unst NK cells for intracellular perforin and GrB contents after an 8‐h co‐incubation with cancer cells. E) Multiplex cytokine/chemokine levels quantified by flow cytometry in the supernatant media. For this, the cancer cells were co‐cultured with IL‐2/TKD‐St NK cells for 8 h. Levels of significance are shown as * p ≤ 0.05, ** p ≤ 0.01.

    Journal: Advanced Science

    Article Title: Crosstalk Between NK Cell Receptors and Tumor Membrane Hsp70‐Derived Peptide: A Combined Computational and Experimental Study

    doi: 10.1002/advs.202305998

    Figure Lengend Snippet: Interaction between mHsp70‐expressing cancer cells and IL‐2/TKD‐St CD94 high and CD94 Low NK cells via ligand‐receptor binding processes. A) Representative fluorescence images of LS174T and UD‐5 cells expressing high densities of membrane Hsp70 (mHsp70) using FITC‐cmHsp70.1 mAb (Green). Nuclei and F‐actin skeleton were stained with DAPI (Blue) and phalloidin (Red), respectively. The scale bars are 15 and 25 µm for LS174T and UD‐5, respectively. B) Representative flow cytometric measurement of mHsp70 expression using FITC‐cmHsp70.1 mAb (Green histograms). Isotype‐matched (Mouse FITC‐IgG1) mAb was used as negative control (Gray histograms). Data are shown as positivity percentage and MFI fold change. The fold change was assessed by dividing the expression value of the FITC‐cmHsp70.1‐treated cells by the values generated for isotype‐matched cells. C) Cancer cell apoptosis detected by Annexin V/PI assay. Prior to measurement, target cells (2 × 10 5 cells well −1 ) were incubated with IL‐2/TKD‐St NK cells at a ratio of 1:1 for 8 h at 37 °C ( n ≥ 3 independent donors, triplicate mean ± SD). D) The positivity ratio of IL‐2/TKD‐St to Unst NK cells for intracellular perforin and GrB contents after an 8‐h co‐incubation with cancer cells. E) Multiplex cytokine/chemokine levels quantified by flow cytometry in the supernatant media. For this, the cancer cells were co‐cultured with IL‐2/TKD‐St NK cells for 8 h. Levels of significance are shown as * p ≤ 0.05, ** p ≤ 0.01.

    Article Snippet: The human colorectal adenocarcinoma cell line LS174T (ATCC CL‐188; ATCC, Manassas, VA, USA) and human head and neck squamous cell carcinoma UD‐SSC 5, (UD‐5; Clinic of Otolaryngology, Düsseldorf, Germany) were cultured in supplemented high glucose (4 g L −1 glucose, Sigma‐Aldrich) Dulbecco's Eagle's Minimum Essential Medium (DMEM).

    Techniques: Expressing, Binding Assay, Fluorescence, Membrane, Staining, Negative Control, Generated, Incubation, Multiplex Assay, Flow Cytometry, Cell Culture

    ( A ) Representative immunofluorescence of LS174T cells treated with DMSO, 10 μM XAV939 or 10 μM Erlotinib as indicated for 24 h. Cells were stained with β-Catenin antibody followed by Alexa488 secondary antibody for visualization and counterstained with DAPI Scale bar = 10 μm. Staining controls are showing in . ( B ) ImageJ quantification of the integrated density of the β-Catenin signal in the nucleus. * ** p = 0.0003; * ** * p ≤ 0.0001, compared to DMSO treatment. ( C ) Representative western blot of LS174T colorectal cancer cell lysates treated with DMSO or 10 μM Erlotinib for 24 h. Blots were probed with the indicated antibodies and β actin was used as a loading control. Uncropped blots and replicates are shown in .

    Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

    Article Title: Zebrafish drug screening identifies Erlotinib as an inhibitor of Wnt/β-catenin signaling and self-renewal in T-cell acute lymphoblastic leukemia

    doi: 10.1016/j.biopha.2023.116013

    Figure Lengend Snippet: ( A ) Representative immunofluorescence of LS174T cells treated with DMSO, 10 μM XAV939 or 10 μM Erlotinib as indicated for 24 h. Cells were stained with β-Catenin antibody followed by Alexa488 secondary antibody for visualization and counterstained with DAPI Scale bar = 10 μm. Staining controls are showing in . ( B ) ImageJ quantification of the integrated density of the β-Catenin signal in the nucleus. * ** p = 0.0003; * ** * p ≤ 0.0001, compared to DMSO treatment. ( C ) Representative western blot of LS174T colorectal cancer cell lysates treated with DMSO or 10 μM Erlotinib for 24 h. Blots were probed with the indicated antibodies and β actin was used as a loading control. Uncropped blots and replicates are shown in .

    Article Snippet: Human cell lines, including Jurkat T-ALL cells (ATCC, VA, USA, TIB-152) and LS174T colon cancer cells (a gift from Professor Hans Clevers and Marc van de Wetering) were cultured at 37 °C in a humidified atmosphere with 5% CO 2 .

    Techniques: Immunofluorescence, Staining, Western Blot