lpd specific raph1 small interfering rna sirna  (Thermo Fisher)


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    siRNA and miRNA Transfection Reagents
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    MIRNA
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    Thermo Fisher lpd specific raph1 small interfering rna sirna
    Depletion of <t>Lpd</t> in HeLa cells using <t>RAPH1</t> <t>siRNA</t> interference significantly reduced the number of plaques formed by L. monocytogenes . The results of Western blot analysis using anti-Lpd antibody against the cell lysate of HeLa cells either transformed with Lpd-expressing plasmid or transfected with 1 nM nonspecific Luc siRNA as a control with RAPH1 individual or mixed siRNAs for 3 days are shown. Tubulin was used as the control. The relative intensity of the band of each sample against HeLa cell lysate was measured using ImageJ. The number of plaques was significantly lower in HeLa cell knockdown of Lpd expression using a RAPH1 siRNA mixture and three of the individual siRNAs. Error bars indicate standard errors. Stars indicate statistically significant differences. *, P

    https://www.bioz.com/result/lpd specific raph1 small interfering rna sirna/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lpd specific raph1 small interfering rna sirna - by Bioz Stars, 2021-06
    97/100 stars

    Images

    1) Product Images from "Lamellipodin Is Important for Cell-to-Cell Spread and Actin-Based Motility in Listeria monocytogenes"

    Article Title: Lamellipodin Is Important for Cell-to-Cell Spread and Actin-Based Motility in Listeria monocytogenes

    Journal: Infection and Immunity

    doi: 10.1128/IAI.00193-15

    Depletion of Lpd in HeLa cells using RAPH1 siRNA interference significantly reduced the number of plaques formed by L. monocytogenes . The results of Western blot analysis using anti-Lpd antibody against the cell lysate of HeLa cells either transformed with Lpd-expressing plasmid or transfected with 1 nM nonspecific Luc siRNA as a control with RAPH1 individual or mixed siRNAs for 3 days are shown. Tubulin was used as the control. The relative intensity of the band of each sample against HeLa cell lysate was measured using ImageJ. The number of plaques was significantly lower in HeLa cell knockdown of Lpd expression using a RAPH1 siRNA mixture and three of the individual siRNAs. Error bars indicate standard errors. Stars indicate statistically significant differences. *, P
    Figure Legend Snippet: Depletion of Lpd in HeLa cells using RAPH1 siRNA interference significantly reduced the number of plaques formed by L. monocytogenes . The results of Western blot analysis using anti-Lpd antibody against the cell lysate of HeLa cells either transformed with Lpd-expressing plasmid or transfected with 1 nM nonspecific Luc siRNA as a control with RAPH1 individual or mixed siRNAs for 3 days are shown. Tubulin was used as the control. The relative intensity of the band of each sample against HeLa cell lysate was measured using ImageJ. The number of plaques was significantly lower in HeLa cell knockdown of Lpd expression using a RAPH1 siRNA mixture and three of the individual siRNAs. Error bars indicate standard errors. Stars indicate statistically significant differences. *, P

    Techniques Used: Western Blot, Transformation Assay, Expressing, Plasmid Preparation, Transfection

    Related Articles

    Plasmid Preparation:

    Article Title: Generation of Inducible CRISPRi and CRISPRa Human Stromal/Stem Cell Lines for Controlled Target Gene Transcription during Lineage Differentiation
    Article Snippet: Lipofectamine 2000 Cell Transfection and Electroporation Transfection To compare the efficiency of gRNAs, siRNA and plasmid inhibition or overexpression in hMSCs utilizing Lipofectamine® 2000 or electroporation were performed to compare with the sgRNA transfection in hMSC-CRISPRi or hMSC-CRISPRa. .. Alkaline phosphatase (ALP) Silencer® Select validated siRNA was purchased from Ambion (App.Bio) (#4390821); pcDNA3-Alkaline phosphatase (ALP) plasmid was purchased from PPL (Public Protein/Plasmid Library, Jiangsu, China, #BC009647). siRNA transfection was performed by Lipofectamine® 2000 (Thermo Fisher Scientific, Roskilde, Denmark) as the manufacturer's instructions suggested for siRNA transfection in cells ( https://assets.thermofisher.com/TFS-assets/LSG/manuals/Lipofectamine_2000_Reag_protocol.pdf ). .. The plasmid transfection in hMSCs was performed by electroporation by Nucleofector™ 2b Device (Lonza, BioNordika Denmark A/S).

    Article Title: Src promotes cutaneous wound healing by regulating MMP-2 through the ERK pathway
    Article Snippet: The DNA contents of the stained nuclei were analyzed, and the number of cells in each cycle phase was calculated. .. In vitro cell migration assays In the present study, the keratinocytes transfected with pcDNA3.1(+)-Src plasmid, Src-siRNA, pcDNA3.1(+), scrambled siRNA (mock group) and untransfected cells (as control), were grown to confluence in 12-well plates in KSFM containing 10 µ g/ml mitomycin C (Invitrogen) for 1 h to completely inhibit cell proliferation. .. A straight scratch was made on the keratinocytes using a P200 pipette tip.

    Article Title: The α-arrestin ARRDC3 mediates ALIX ubiquitination and G protein–coupled receptor lysosomal sorting
    Article Snippet: .. siRNA and cDNA transfections HeLa cells were transfected with plasmid DNA using 1 mg/ml polyethylenimine (PEI) at a 6:1 ratio (6 μl PEI:1 μg plasmid). siRNA transfection was performed using Oligofectamine (Invitrogen) per manufacturer’s instructions. .. All single siRNAs were purchased from Qiagen or Invitrogen: nonspecific, 5′-GGCTACGTCCAGGAGCGCACC-3′; ALIX #1, 5′-AAGTACCTCAGTCTATATTGA-3′; ALIX #3, 5′-AATCGAGACGCTCCTGAGATA-3′; ARRDC3, 5′-AAGGGAAAATGAA­GG­AAGTAA-3′; NEDD4.1, 5′-CCGGAGAATTATGGGTGTCAA-3′; NEDD4.2, 5′-AAGATCATAACACAAAGACTA-3′; WWP1, 5′-AAGAA­GTCATCTGTAACTAAA-3′; WWP2, 5′-TAGACACGTCCGTTGGG­CAGCTCTC-3′; SMURF1, 5′-GCATCGAAGTGTCCAGAGAAG-3′; SMURF2, 5′-CCGGATCTTCACAACAGTTTA-3′; NEDL1, 5′-AAGATGAGGTCTTGTCCGAAA-3′; and NEDL2, 5′-AAGTGGGTACCTCCAGTTTAA-3′.

    Transfection:

    Article Title: Generation of Inducible CRISPRi and CRISPRa Human Stromal/Stem Cell Lines for Controlled Target Gene Transcription during Lineage Differentiation
    Article Snippet: Lipofectamine 2000 Cell Transfection and Electroporation Transfection To compare the efficiency of gRNAs, siRNA and plasmid inhibition or overexpression in hMSCs utilizing Lipofectamine® 2000 or electroporation were performed to compare with the sgRNA transfection in hMSC-CRISPRi or hMSC-CRISPRa. .. Alkaline phosphatase (ALP) Silencer® Select validated siRNA was purchased from Ambion (App.Bio) (#4390821); pcDNA3-Alkaline phosphatase (ALP) plasmid was purchased from PPL (Public Protein/Plasmid Library, Jiangsu, China, #BC009647). siRNA transfection was performed by Lipofectamine® 2000 (Thermo Fisher Scientific, Roskilde, Denmark) as the manufacturer's instructions suggested for siRNA transfection in cells ( https://assets.thermofisher.com/TFS-assets/LSG/manuals/Lipofectamine_2000_Reag_protocol.pdf ). .. The plasmid transfection in hMSCs was performed by electroporation by Nucleofector™ 2b Device (Lonza, BioNordika Denmark A/S).

    Article Title: Src promotes cutaneous wound healing by regulating MMP-2 through the ERK pathway
    Article Snippet: The DNA contents of the stained nuclei were analyzed, and the number of cells in each cycle phase was calculated. .. In vitro cell migration assays In the present study, the keratinocytes transfected with pcDNA3.1(+)-Src plasmid, Src-siRNA, pcDNA3.1(+), scrambled siRNA (mock group) and untransfected cells (as control), were grown to confluence in 12-well plates in KSFM containing 10 µ g/ml mitomycin C (Invitrogen) for 1 h to completely inhibit cell proliferation. .. A straight scratch was made on the keratinocytes using a P200 pipette tip.

    Article Title: The α-arrestin ARRDC3 mediates ALIX ubiquitination and G protein–coupled receptor lysosomal sorting
    Article Snippet: .. siRNA and cDNA transfections HeLa cells were transfected with plasmid DNA using 1 mg/ml polyethylenimine (PEI) at a 6:1 ratio (6 μl PEI:1 μg plasmid). siRNA transfection was performed using Oligofectamine (Invitrogen) per manufacturer’s instructions. .. All single siRNAs were purchased from Qiagen or Invitrogen: nonspecific, 5′-GGCTACGTCCAGGAGCGCACC-3′; ALIX #1, 5′-AAGTACCTCAGTCTATATTGA-3′; ALIX #3, 5′-AATCGAGACGCTCCTGAGATA-3′; ARRDC3, 5′-AAGGGAAAATGAA­GG­AAGTAA-3′; NEDD4.1, 5′-CCGGAGAATTATGGGTGTCAA-3′; NEDD4.2, 5′-AAGATCATAACACAAAGACTA-3′; WWP1, 5′-AAGAA­GTCATCTGTAACTAAA-3′; WWP2, 5′-TAGACACGTCCGTTGGG­CAGCTCTC-3′; SMURF1, 5′-GCATCGAAGTGTCCAGAGAAG-3′; SMURF2, 5′-CCGGATCTTCACAACAGTTTA-3′; NEDL1, 5′-AAGATGAGGTCTTGTCCGAAA-3′; and NEDL2, 5′-AAGTGGGTACCTCCAGTTTAA-3′.

    In Vitro:

    Article Title: Src promotes cutaneous wound healing by regulating MMP-2 through the ERK pathway
    Article Snippet: The DNA contents of the stained nuclei were analyzed, and the number of cells in each cycle phase was calculated. .. In vitro cell migration assays In the present study, the keratinocytes transfected with pcDNA3.1(+)-Src plasmid, Src-siRNA, pcDNA3.1(+), scrambled siRNA (mock group) and untransfected cells (as control), were grown to confluence in 12-well plates in KSFM containing 10 µ g/ml mitomycin C (Invitrogen) for 1 h to completely inhibit cell proliferation. .. A straight scratch was made on the keratinocytes using a P200 pipette tip.

    Migration:

    Article Title: Src promotes cutaneous wound healing by regulating MMP-2 through the ERK pathway
    Article Snippet: The DNA contents of the stained nuclei were analyzed, and the number of cells in each cycle phase was calculated. .. In vitro cell migration assays In the present study, the keratinocytes transfected with pcDNA3.1(+)-Src plasmid, Src-siRNA, pcDNA3.1(+), scrambled siRNA (mock group) and untransfected cells (as control), were grown to confluence in 12-well plates in KSFM containing 10 µ g/ml mitomycin C (Invitrogen) for 1 h to completely inhibit cell proliferation. .. A straight scratch was made on the keratinocytes using a P200 pipette tip.

    Polymerase Chain Reaction:

    Article Title: MicroRNA signature of inflamed lymphatic endothelium and role of miR-9 in lymphangiogenesis and inflammation
    Article Snippet: The cDNA was mixed with RT2 SYBR Green/ROX qPCR Mastermix, and the mixture was added to a 384-well RT2 miRNA PCR array (SABiosciences, Valencia, CA), including predefined primer pairs for a set of 88 human miRNAs that are either documented or predicted to have targets involved in the regulation of immunity and inflammatory responses, in addition to a panel of 8 housekeeping genes and controls. .. All the real-time PCR-based experiments using the RT2 miRNA PCR array were performed in triplicate using a sequence detection system (ABI Prism 7900 HT, Applied Biosystems, Foster City, CA) according to the manufacturer's instructions. ..

    Sequencing:

    Article Title: MicroRNA signature of inflamed lymphatic endothelium and role of miR-9 in lymphangiogenesis and inflammation
    Article Snippet: The cDNA was mixed with RT2 SYBR Green/ROX qPCR Mastermix, and the mixture was added to a 384-well RT2 miRNA PCR array (SABiosciences, Valencia, CA), including predefined primer pairs for a set of 88 human miRNAs that are either documented or predicted to have targets involved in the regulation of immunity and inflammatory responses, in addition to a panel of 8 housekeeping genes and controls. .. All the real-time PCR-based experiments using the RT2 miRNA PCR array were performed in triplicate using a sequence detection system (ABI Prism 7900 HT, Applied Biosystems, Foster City, CA) according to the manufacturer's instructions. ..

    Synthesized:

    Article Title: Ineffective delivery of diet-derived microRNAs to recipient animal organisms
    Article Snippet: .. To that mixture, 2 ficomoles were added of a chemically synthesized miRNA duplex mimic of hsa-microRNA-422b (hsa-miR-422b) (Ambion/Applied Biosystems) for quantitative normalization of miRNA plasma levels. ..

    Binding Assay:

    Article Title: Increased siRNA duplex stability correlates with reduced off-target and elevated on-target effects
    Article Snippet: His-Ago2 was then purified by Ni2+ -affinity chromatography followed by gel-filtration (Superdex200 prep grade, GE-Healthcare). .. For the Ago1-siRNA binding assay, siRNA#2 guide strand was radioactively labeled by PNK (Fermentas)-mediated 5′-phosphorylation using 32 P-γ-ATP (Hartmann Analytics). ..

    Labeling:

    Article Title: Increased siRNA duplex stability correlates with reduced off-target and elevated on-target effects
    Article Snippet: His-Ago2 was then purified by Ni2+ -affinity chromatography followed by gel-filtration (Superdex200 prep grade, GE-Healthcare). .. For the Ago1-siRNA binding assay, siRNA#2 guide strand was radioactively labeled by PNK (Fermentas)-mediated 5′-phosphorylation using 32 P-γ-ATP (Hartmann Analytics). ..

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    Thermo Fisher lpd specific raph1 small interfering rna sirna
    Depletion of <t>Lpd</t> in HeLa cells using <t>RAPH1</t> <t>siRNA</t> interference significantly reduced the number of plaques formed by L. monocytogenes . The results of Western blot analysis using anti-Lpd antibody against the cell lysate of HeLa cells either transformed with Lpd-expressing plasmid or transfected with 1 nM nonspecific Luc siRNA as a control with RAPH1 individual or mixed siRNAs for 3 days are shown. Tubulin was used as the control. The relative intensity of the band of each sample against HeLa cell lysate was measured using ImageJ. The number of plaques was significantly lower in HeLa cell knockdown of Lpd expression using a RAPH1 siRNA mixture and three of the individual siRNAs. Error bars indicate standard errors. Stars indicate statistically significant differences. *, P
    Lpd Specific Raph1 Small Interfering Rna Sirna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lpd specific raph1 small interfering rna sirna/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lpd specific raph1 small interfering rna sirna - by Bioz Stars, 2021-06
    97/100 stars
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    Depletion of Lpd in HeLa cells using RAPH1 siRNA interference significantly reduced the number of plaques formed by L. monocytogenes . The results of Western blot analysis using anti-Lpd antibody against the cell lysate of HeLa cells either transformed with Lpd-expressing plasmid or transfected with 1 nM nonspecific Luc siRNA as a control with RAPH1 individual or mixed siRNAs for 3 days are shown. Tubulin was used as the control. The relative intensity of the band of each sample against HeLa cell lysate was measured using ImageJ. The number of plaques was significantly lower in HeLa cell knockdown of Lpd expression using a RAPH1 siRNA mixture and three of the individual siRNAs. Error bars indicate standard errors. Stars indicate statistically significant differences. *, P

    Journal: Infection and Immunity

    Article Title: Lamellipodin Is Important for Cell-to-Cell Spread and Actin-Based Motility in Listeria monocytogenes

    doi: 10.1128/IAI.00193-15

    Figure Lengend Snippet: Depletion of Lpd in HeLa cells using RAPH1 siRNA interference significantly reduced the number of plaques formed by L. monocytogenes . The results of Western blot analysis using anti-Lpd antibody against the cell lysate of HeLa cells either transformed with Lpd-expressing plasmid or transfected with 1 nM nonspecific Luc siRNA as a control with RAPH1 individual or mixed siRNAs for 3 days are shown. Tubulin was used as the control. The relative intensity of the band of each sample against HeLa cell lysate was measured using ImageJ. The number of plaques was significantly lower in HeLa cell knockdown of Lpd expression using a RAPH1 siRNA mixture and three of the individual siRNAs. Error bars indicate standard errors. Stars indicate statistically significant differences. *, P

    Article Snippet: Lpd RNA interference (RNAi) was performed using Interferin (Polyplus)-mediated delivery of Lpd-specific RAPH1 small interfering RNA (siRNA) (Thermo Scientific).

    Techniques: Western Blot, Transformation Assay, Expressing, Plasmid Preparation, Transfection