lp6 c  (ATCC)


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    Name:
    Mortierella hyalina
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    Catalog Number:
    64258
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    Structured Review

    ATCC lp6 c
    Apolar and polar lipids after incubation of PC with CCCS of L. pneumophila . PC was incubated with the CCCS of different L. pneumophila strains for 16 h. Apolar (A) and polar (B) lipids were characterized by TLC. A representative experiment is shown. Abbreviations: St, standard (1A, 1,2-dipalmitoylglycerol, palmitic acid; 1B, LPC, dipalmitoylphosphatidylcholine); C, negative control (concentrated BYE broth or concentrated −P broth, respectively); P, positive control (PLC from C. perfringens ); 1, Lp1-MM6; 2, Lp1-MRC; 3, Lp2-ATCC; 4, <t>Lp6-c;</t> 5, Lp12-ATCC.

    https://www.bioz.com/result/lp6 c/product/ATCC
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    lp6 c - by Bioz Stars, 2021-07
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    Images

    1) Product Images from "Novel Phospholipase A Activity Secreted by Legionella Species"

    Article Title: Novel Phospholipase A Activity Secreted by Legionella Species

    Journal: Journal of Bacteriology

    doi:

    Apolar and polar lipids after incubation of PC with CCCS of L. pneumophila . PC was incubated with the CCCS of different L. pneumophila strains for 16 h. Apolar (A) and polar (B) lipids were characterized by TLC. A representative experiment is shown. Abbreviations: St, standard (1A, 1,2-dipalmitoylglycerol, palmitic acid; 1B, LPC, dipalmitoylphosphatidylcholine); C, negative control (concentrated BYE broth or concentrated −P broth, respectively); P, positive control (PLC from C. perfringens ); 1, Lp1-MM6; 2, Lp1-MRC; 3, Lp2-ATCC; 4, Lp6-c; 5, Lp12-ATCC.
    Figure Legend Snippet: Apolar and polar lipids after incubation of PC with CCCS of L. pneumophila . PC was incubated with the CCCS of different L. pneumophila strains for 16 h. Apolar (A) and polar (B) lipids were characterized by TLC. A representative experiment is shown. Abbreviations: St, standard (1A, 1,2-dipalmitoylglycerol, palmitic acid; 1B, LPC, dipalmitoylphosphatidylcholine); C, negative control (concentrated BYE broth or concentrated −P broth, respectively); P, positive control (PLC from C. perfringens ); 1, Lp1-MM6; 2, Lp1-MRC; 3, Lp2-ATCC; 4, Lp6-c; 5, Lp12-ATCC.

    Techniques Used: Incubation, Thin Layer Chromatography, Negative Control, Positive Control, Planar Chromatography

    Characterization of AEC fractions. CCCS of Lp6-c was applied onto an AEC column. Fractions eluted by 0.1 to 0.7 M sodium chloride were investigated for protein content, hydrolysis of p -NPPC (OD 410 ), and hydrolysis of phosphomonoesters (P i content). A representative experiment is shown.
    Figure Legend Snippet: Characterization of AEC fractions. CCCS of Lp6-c was applied onto an AEC column. Fractions eluted by 0.1 to 0.7 M sodium chloride were investigated for protein content, hydrolysis of p -NPPC (OD 410 ), and hydrolysis of phosphomonoesters (P i content). A representative experiment is shown.

    Techniques Used:

    ESI-MS. CCCS of Lp6-c was incubated with Alveofact for 16 h. Lipids were separated from the water-soluble substances by lipid extraction. Water-soluble substances were analyzed by ESI-MS. A representative experiment is shown.
    Figure Legend Snippet: ESI-MS. CCCS of Lp6-c was incubated with Alveofact for 16 h. Lipids were separated from the water-soluble substances by lipid extraction. Water-soluble substances were analyzed by ESI-MS. A representative experiment is shown.

    Techniques Used: Mass Spectrometry, Incubation

    2) Product Images from "Novel Phospholipase A Activity Secreted by Legionella Species"

    Article Title: Novel Phospholipase A Activity Secreted by Legionella Species

    Journal: Journal of Bacteriology

    doi:

    Apolar and polar lipids after incubation of PC with CCCS of L. pneumophila . PC was incubated with the CCCS of different L. pneumophila strains for 16 h. Apolar (A) and polar (B) lipids were characterized by TLC. A representative experiment is shown. Abbreviations: St, standard (1A, 1,2-dipalmitoylglycerol, palmitic acid; 1B, LPC, dipalmitoylphosphatidylcholine); C, negative control (concentrated BYE broth or concentrated −P broth, respectively); P, positive control (PLC from C. perfringens ); 1, Lp1-MM6; 2, Lp1-MRC; 3, Lp2-ATCC; 4, Lp6-c; 5, Lp12-ATCC.
    Figure Legend Snippet: Apolar and polar lipids after incubation of PC with CCCS of L. pneumophila . PC was incubated with the CCCS of different L. pneumophila strains for 16 h. Apolar (A) and polar (B) lipids were characterized by TLC. A representative experiment is shown. Abbreviations: St, standard (1A, 1,2-dipalmitoylglycerol, palmitic acid; 1B, LPC, dipalmitoylphosphatidylcholine); C, negative control (concentrated BYE broth or concentrated −P broth, respectively); P, positive control (PLC from C. perfringens ); 1, Lp1-MM6; 2, Lp1-MRC; 3, Lp2-ATCC; 4, Lp6-c; 5, Lp12-ATCC.

    Techniques Used: Incubation, Thin Layer Chromatography, Negative Control, Positive Control, Planar Chromatography

    Characterization of AEC fractions. CCCS of Lp6-c was applied onto an AEC column. Fractions eluted by 0.1 to 0.7 M sodium chloride were investigated for protein content, hydrolysis of p -NPPC (OD 410 ), and hydrolysis of phosphomonoesters (P i content). A representative experiment is shown.
    Figure Legend Snippet: Characterization of AEC fractions. CCCS of Lp6-c was applied onto an AEC column. Fractions eluted by 0.1 to 0.7 M sodium chloride were investigated for protein content, hydrolysis of p -NPPC (OD 410 ), and hydrolysis of phosphomonoesters (P i content). A representative experiment is shown.

    Techniques Used:

    ESI-MS. CCCS of Lp6-c was incubated with Alveofact for 16 h. Lipids were separated from the water-soluble substances by lipid extraction. Water-soluble substances were analyzed by ESI-MS. A representative experiment is shown.
    Figure Legend Snippet: ESI-MS. CCCS of Lp6-c was incubated with Alveofact for 16 h. Lipids were separated from the water-soluble substances by lipid extraction. Water-soluble substances were analyzed by ESI-MS. A representative experiment is shown.

    Techniques Used: Mass Spectrometry, Incubation

    3) Product Images from "Novel Phospholipase A Activity Secreted by Legionella Species"

    Article Title: Novel Phospholipase A Activity Secreted by Legionella Species

    Journal: Journal of Bacteriology

    doi:

    Apolar and polar lipids after incubation of PC with CCCS of L. pneumophila . PC was incubated with the CCCS of different L. pneumophila strains for 16 h. Apolar (A) and polar (B) lipids were characterized by TLC. A representative experiment is shown. Abbreviations: St, standard (1A, 1,2-dipalmitoylglycerol, palmitic acid; 1B, LPC, dipalmitoylphosphatidylcholine); C, negative control (concentrated BYE broth or concentrated −P broth, respectively); P, positive control (PLC from C. perfringens ); 1, Lp1-MM6; 2, Lp1-MRC; 3, Lp2-ATCC; 4, Lp6-c; 5, Lp12-ATCC.
    Figure Legend Snippet: Apolar and polar lipids after incubation of PC with CCCS of L. pneumophila . PC was incubated with the CCCS of different L. pneumophila strains for 16 h. Apolar (A) and polar (B) lipids were characterized by TLC. A representative experiment is shown. Abbreviations: St, standard (1A, 1,2-dipalmitoylglycerol, palmitic acid; 1B, LPC, dipalmitoylphosphatidylcholine); C, negative control (concentrated BYE broth or concentrated −P broth, respectively); P, positive control (PLC from C. perfringens ); 1, Lp1-MM6; 2, Lp1-MRC; 3, Lp2-ATCC; 4, Lp6-c; 5, Lp12-ATCC.

    Techniques Used: Incubation, Thin Layer Chromatography, Negative Control, Positive Control, Planar Chromatography

    Characterization of AEC fractions. CCCS of Lp6-c was applied onto an AEC column. Fractions eluted by 0.1 to 0.7 M sodium chloride were investigated for protein content, hydrolysis of p -NPPC (OD 410 ), and hydrolysis of phosphomonoesters (P i content). A representative experiment is shown.
    Figure Legend Snippet: Characterization of AEC fractions. CCCS of Lp6-c was applied onto an AEC column. Fractions eluted by 0.1 to 0.7 M sodium chloride were investigated for protein content, hydrolysis of p -NPPC (OD 410 ), and hydrolysis of phosphomonoesters (P i content). A representative experiment is shown.

    Techniques Used:

    ESI-MS. CCCS of Lp6-c was incubated with Alveofact for 16 h. Lipids were separated from the water-soluble substances by lipid extraction. Water-soluble substances were analyzed by ESI-MS. A representative experiment is shown.
    Figure Legend Snippet: ESI-MS. CCCS of Lp6-c was incubated with Alveofact for 16 h. Lipids were separated from the water-soluble substances by lipid extraction. Water-soluble substances were analyzed by ESI-MS. A representative experiment is shown.

    Techniques Used: Mass Spectrometry, Incubation

    4) Product Images from "Novel Phospholipase A Activity Secreted by Legionella Species"

    Article Title: Novel Phospholipase A Activity Secreted by Legionella Species

    Journal: Journal of Bacteriology

    doi:

    Apolar and polar lipids after incubation of PC with CCCS of L. pneumophila . PC was incubated with the CCCS of different L. pneumophila strains for 16 h. Apolar (A) and polar (B) lipids were characterized by TLC. A representative experiment is shown. Abbreviations: St, standard (1A, 1,2-dipalmitoylglycerol, palmitic acid; 1B, LPC, dipalmitoylphosphatidylcholine); C, negative control (concentrated BYE broth or concentrated −P broth, respectively); P, positive control (PLC from C. perfringens ); 1, Lp1-MM6; 2, Lp1-MRC; 3, Lp2-ATCC; 4, Lp6-c; 5, Lp12-ATCC.
    Figure Legend Snippet: Apolar and polar lipids after incubation of PC with CCCS of L. pneumophila . PC was incubated with the CCCS of different L. pneumophila strains for 16 h. Apolar (A) and polar (B) lipids were characterized by TLC. A representative experiment is shown. Abbreviations: St, standard (1A, 1,2-dipalmitoylglycerol, palmitic acid; 1B, LPC, dipalmitoylphosphatidylcholine); C, negative control (concentrated BYE broth or concentrated −P broth, respectively); P, positive control (PLC from C. perfringens ); 1, Lp1-MM6; 2, Lp1-MRC; 3, Lp2-ATCC; 4, Lp6-c; 5, Lp12-ATCC.

    Techniques Used: Incubation, Thin Layer Chromatography, Negative Control, Positive Control, Planar Chromatography

    Characterization of AEC fractions. CCCS of Lp6-c was applied onto an AEC column. Fractions eluted by 0.1 to 0.7 M sodium chloride were investigated for protein content, hydrolysis of p -NPPC (OD 410 ), and hydrolysis of phosphomonoesters (P i content). A representative experiment is shown.
    Figure Legend Snippet: Characterization of AEC fractions. CCCS of Lp6-c was applied onto an AEC column. Fractions eluted by 0.1 to 0.7 M sodium chloride were investigated for protein content, hydrolysis of p -NPPC (OD 410 ), and hydrolysis of phosphomonoesters (P i content). A representative experiment is shown.

    Techniques Used:

    ESI-MS. CCCS of Lp6-c was incubated with Alveofact for 16 h. Lipids were separated from the water-soluble substances by lipid extraction. Water-soluble substances were analyzed by ESI-MS. A representative experiment is shown.
    Figure Legend Snippet: ESI-MS. CCCS of Lp6-c was incubated with Alveofact for 16 h. Lipids were separated from the water-soluble substances by lipid extraction. Water-soluble substances were analyzed by ESI-MS. A representative experiment is shown.

    Techniques Used: Mass Spectrometry, Incubation

    5) Product Images from "Novel Phospholipase A Activity Secreted by Legionella Species"

    Article Title: Novel Phospholipase A Activity Secreted by Legionella Species

    Journal: Journal of Bacteriology

    doi:

    Apolar and polar lipids after incubation of PC with CCCS of L. pneumophila . PC was incubated with the CCCS of different L. pneumophila strains for 16 h. Apolar (A) and polar (B) lipids were characterized by TLC. A representative experiment is shown. Abbreviations: St, standard (1A, 1,2-dipalmitoylglycerol, palmitic acid; 1B, LPC, dipalmitoylphosphatidylcholine); C, negative control (concentrated BYE broth or concentrated −P broth, respectively); P, positive control (PLC from C. perfringens ); 1, Lp1-MM6; 2, Lp1-MRC; 3, Lp2-ATCC; 4, Lp6-c; 5, Lp12-ATCC.
    Figure Legend Snippet: Apolar and polar lipids after incubation of PC with CCCS of L. pneumophila . PC was incubated with the CCCS of different L. pneumophila strains for 16 h. Apolar (A) and polar (B) lipids were characterized by TLC. A representative experiment is shown. Abbreviations: St, standard (1A, 1,2-dipalmitoylglycerol, palmitic acid; 1B, LPC, dipalmitoylphosphatidylcholine); C, negative control (concentrated BYE broth or concentrated −P broth, respectively); P, positive control (PLC from C. perfringens ); 1, Lp1-MM6; 2, Lp1-MRC; 3, Lp2-ATCC; 4, Lp6-c; 5, Lp12-ATCC.

    Techniques Used: Incubation, Thin Layer Chromatography, Negative Control, Positive Control, Planar Chromatography

    Characterization of AEC fractions. CCCS of Lp6-c was applied onto an AEC column. Fractions eluted by 0.1 to 0.7 M sodium chloride were investigated for protein content, hydrolysis of p -NPPC (OD 410 ), and hydrolysis of phosphomonoesters (P i content). A representative experiment is shown.
    Figure Legend Snippet: Characterization of AEC fractions. CCCS of Lp6-c was applied onto an AEC column. Fractions eluted by 0.1 to 0.7 M sodium chloride were investigated for protein content, hydrolysis of p -NPPC (OD 410 ), and hydrolysis of phosphomonoesters (P i content). A representative experiment is shown.

    Techniques Used:

    ESI-MS. CCCS of Lp6-c was incubated with Alveofact for 16 h. Lipids were separated from the water-soluble substances by lipid extraction. Water-soluble substances were analyzed by ESI-MS. A representative experiment is shown.
    Figure Legend Snippet: ESI-MS. CCCS of Lp6-c was incubated with Alveofact for 16 h. Lipids were separated from the water-soluble substances by lipid extraction. Water-soluble substances were analyzed by ESI-MS. A representative experiment is shown.

    Techniques Used: Mass Spectrometry, Incubation

    6) Product Images from "Novel Phospholipase A Activity Secreted by Legionella Species"

    Article Title: Novel Phospholipase A Activity Secreted by Legionella Species

    Journal: Journal of Bacteriology

    doi:

    Apolar and polar lipids after incubation of PC with CCCS of L. pneumophila . PC was incubated with the CCCS of different L. pneumophila strains for 16 h. Apolar (A) and polar (B) lipids were characterized by TLC. A representative experiment is shown. Abbreviations: St, standard (1A, 1,2-dipalmitoylglycerol, palmitic acid; 1B, LPC, dipalmitoylphosphatidylcholine); C, negative control (concentrated BYE broth or concentrated −P broth, respectively); P, positive control (PLC from C. perfringens ); 1, Lp1-MM6; 2, Lp1-MRC; 3, Lp2-ATCC; 4, Lp6-c; 5, Lp12-ATCC.
    Figure Legend Snippet: Apolar and polar lipids after incubation of PC with CCCS of L. pneumophila . PC was incubated with the CCCS of different L. pneumophila strains for 16 h. Apolar (A) and polar (B) lipids were characterized by TLC. A representative experiment is shown. Abbreviations: St, standard (1A, 1,2-dipalmitoylglycerol, palmitic acid; 1B, LPC, dipalmitoylphosphatidylcholine); C, negative control (concentrated BYE broth or concentrated −P broth, respectively); P, positive control (PLC from C. perfringens ); 1, Lp1-MM6; 2, Lp1-MRC; 3, Lp2-ATCC; 4, Lp6-c; 5, Lp12-ATCC.

    Techniques Used: Incubation, Thin Layer Chromatography, Negative Control, Positive Control, Planar Chromatography

    Characterization of AEC fractions. CCCS of Lp6-c was applied onto an AEC column. Fractions eluted by 0.1 to 0.7 M sodium chloride were investigated for protein content, hydrolysis of p -NPPC (OD 410 ), and hydrolysis of phosphomonoesters (P i content). A representative experiment is shown.
    Figure Legend Snippet: Characterization of AEC fractions. CCCS of Lp6-c was applied onto an AEC column. Fractions eluted by 0.1 to 0.7 M sodium chloride were investigated for protein content, hydrolysis of p -NPPC (OD 410 ), and hydrolysis of phosphomonoesters (P i content). A representative experiment is shown.

    Techniques Used:

    ESI-MS. CCCS of Lp6-c was incubated with Alveofact for 16 h. Lipids were separated from the water-soluble substances by lipid extraction. Water-soluble substances were analyzed by ESI-MS. A representative experiment is shown.
    Figure Legend Snippet: ESI-MS. CCCS of Lp6-c was incubated with Alveofact for 16 h. Lipids were separated from the water-soluble substances by lipid extraction. Water-soluble substances were analyzed by ESI-MS. A representative experiment is shown.

    Techniques Used: Mass Spectrometry, Incubation

    7) Product Images from "Novel Phospholipase A Activity Secreted by Legionella Species"

    Article Title: Novel Phospholipase A Activity Secreted by Legionella Species

    Journal: Journal of Bacteriology

    doi:

    Apolar and polar lipids after incubation of PC with CCCS of L. pneumophila . PC was incubated with the CCCS of different L. pneumophila strains for 16 h. Apolar (A) and polar (B) lipids were characterized by TLC. A representative experiment is shown. Abbreviations: St, standard (1A, 1,2-dipalmitoylglycerol, palmitic acid; 1B, LPC, dipalmitoylphosphatidylcholine); C, negative control (concentrated BYE broth or concentrated −P broth, respectively); P, positive control (PLC from C. perfringens ); 1, Lp1-MM6; 2, Lp1-MRC; 3, Lp2-ATCC; 4, Lp6-c; 5, Lp12-ATCC.
    Figure Legend Snippet: Apolar and polar lipids after incubation of PC with CCCS of L. pneumophila . PC was incubated with the CCCS of different L. pneumophila strains for 16 h. Apolar (A) and polar (B) lipids were characterized by TLC. A representative experiment is shown. Abbreviations: St, standard (1A, 1,2-dipalmitoylglycerol, palmitic acid; 1B, LPC, dipalmitoylphosphatidylcholine); C, negative control (concentrated BYE broth or concentrated −P broth, respectively); P, positive control (PLC from C. perfringens ); 1, Lp1-MM6; 2, Lp1-MRC; 3, Lp2-ATCC; 4, Lp6-c; 5, Lp12-ATCC.

    Techniques Used: Incubation, Thin Layer Chromatography, Negative Control, Positive Control, Planar Chromatography

    Characterization of AEC fractions. CCCS of Lp6-c was applied onto an AEC column. Fractions eluted by 0.1 to 0.7 M sodium chloride were investigated for protein content, hydrolysis of p -NPPC (OD 410 ), and hydrolysis of phosphomonoesters (P i content). A representative experiment is shown.
    Figure Legend Snippet: Characterization of AEC fractions. CCCS of Lp6-c was applied onto an AEC column. Fractions eluted by 0.1 to 0.7 M sodium chloride were investigated for protein content, hydrolysis of p -NPPC (OD 410 ), and hydrolysis of phosphomonoesters (P i content). A representative experiment is shown.

    Techniques Used:

    ESI-MS. CCCS of Lp6-c was incubated with Alveofact for 16 h. Lipids were separated from the water-soluble substances by lipid extraction. Water-soluble substances were analyzed by ESI-MS. A representative experiment is shown.
    Figure Legend Snippet: ESI-MS. CCCS of Lp6-c was incubated with Alveofact for 16 h. Lipids were separated from the water-soluble substances by lipid extraction. Water-soluble substances were analyzed by ESI-MS. A representative experiment is shown.

    Techniques Used: Mass Spectrometry, Incubation

    8) Product Images from "Novel Phospholipase A Activity Secreted by Legionella Species"

    Article Title: Novel Phospholipase A Activity Secreted by Legionella Species

    Journal: Journal of Bacteriology

    doi:

    Apolar and polar lipids after incubation of PC with CCCS of L. pneumophila . PC was incubated with the CCCS of different L. pneumophila strains for 16 h. Apolar (A) and polar (B) lipids were characterized by TLC. A representative experiment is shown. Abbreviations: St, standard (1A, 1,2-dipalmitoylglycerol, palmitic acid; 1B, LPC, dipalmitoylphosphatidylcholine); C, negative control (concentrated BYE broth or concentrated −P broth, respectively); P, positive control (PLC from C. perfringens ); 1, Lp1-MM6; 2, Lp1-MRC; 3, Lp2-ATCC; 4, Lp6-c; 5, Lp12-ATCC.
    Figure Legend Snippet: Apolar and polar lipids after incubation of PC with CCCS of L. pneumophila . PC was incubated with the CCCS of different L. pneumophila strains for 16 h. Apolar (A) and polar (B) lipids were characterized by TLC. A representative experiment is shown. Abbreviations: St, standard (1A, 1,2-dipalmitoylglycerol, palmitic acid; 1B, LPC, dipalmitoylphosphatidylcholine); C, negative control (concentrated BYE broth or concentrated −P broth, respectively); P, positive control (PLC from C. perfringens ); 1, Lp1-MM6; 2, Lp1-MRC; 3, Lp2-ATCC; 4, Lp6-c; 5, Lp12-ATCC.

    Techniques Used: Incubation, Thin Layer Chromatography, Negative Control, Positive Control, Planar Chromatography

    Characterization of AEC fractions. CCCS of Lp6-c was applied onto an AEC column. Fractions eluted by 0.1 to 0.7 M sodium chloride were investigated for protein content, hydrolysis of p -NPPC (OD 410 ), and hydrolysis of phosphomonoesters (P i content). A representative experiment is shown.
    Figure Legend Snippet: Characterization of AEC fractions. CCCS of Lp6-c was applied onto an AEC column. Fractions eluted by 0.1 to 0.7 M sodium chloride were investigated for protein content, hydrolysis of p -NPPC (OD 410 ), and hydrolysis of phosphomonoesters (P i content). A representative experiment is shown.

    Techniques Used:

    ESI-MS. CCCS of Lp6-c was incubated with Alveofact for 16 h. Lipids were separated from the water-soluble substances by lipid extraction. Water-soluble substances were analyzed by ESI-MS. A representative experiment is shown.
    Figure Legend Snippet: ESI-MS. CCCS of Lp6-c was incubated with Alveofact for 16 h. Lipids were separated from the water-soluble substances by lipid extraction. Water-soluble substances were analyzed by ESI-MS. A representative experiment is shown.

    Techniques Used: Mass Spectrometry, Incubation

    9) Product Images from "Novel Phospholipase A Activity Secreted by Legionella Species"

    Article Title: Novel Phospholipase A Activity Secreted by Legionella Species

    Journal: Journal of Bacteriology

    doi:

    Apolar and polar lipids after incubation of PC with CCCS of L. pneumophila . PC was incubated with the CCCS of different L. pneumophila strains for 16 h. Apolar (A) and polar (B) lipids were characterized by TLC. A representative experiment is shown. Abbreviations: St, standard (1A, 1,2-dipalmitoylglycerol, palmitic acid; 1B, LPC, dipalmitoylphosphatidylcholine); C, negative control (concentrated BYE broth or concentrated −P broth, respectively); P, positive control (PLC from C. perfringens ); 1, Lp1-MM6; 2, Lp1-MRC; 3, Lp2-ATCC; 4, Lp6-c; 5, Lp12-ATCC.
    Figure Legend Snippet: Apolar and polar lipids after incubation of PC with CCCS of L. pneumophila . PC was incubated with the CCCS of different L. pneumophila strains for 16 h. Apolar (A) and polar (B) lipids were characterized by TLC. A representative experiment is shown. Abbreviations: St, standard (1A, 1,2-dipalmitoylglycerol, palmitic acid; 1B, LPC, dipalmitoylphosphatidylcholine); C, negative control (concentrated BYE broth or concentrated −P broth, respectively); P, positive control (PLC from C. perfringens ); 1, Lp1-MM6; 2, Lp1-MRC; 3, Lp2-ATCC; 4, Lp6-c; 5, Lp12-ATCC.

    Techniques Used: Incubation, Thin Layer Chromatography, Negative Control, Positive Control, Planar Chromatography

    Characterization of AEC fractions. CCCS of Lp6-c was applied onto an AEC column. Fractions eluted by 0.1 to 0.7 M sodium chloride were investigated for protein content, hydrolysis of p -NPPC (OD 410 ), and hydrolysis of phosphomonoesters (P i content). A representative experiment is shown.
    Figure Legend Snippet: Characterization of AEC fractions. CCCS of Lp6-c was applied onto an AEC column. Fractions eluted by 0.1 to 0.7 M sodium chloride were investigated for protein content, hydrolysis of p -NPPC (OD 410 ), and hydrolysis of phosphomonoesters (P i content). A representative experiment is shown.

    Techniques Used:

    ESI-MS. CCCS of Lp6-c was incubated with Alveofact for 16 h. Lipids were separated from the water-soluble substances by lipid extraction. Water-soluble substances were analyzed by ESI-MS. A representative experiment is shown.
    Figure Legend Snippet: ESI-MS. CCCS of Lp6-c was incubated with Alveofact for 16 h. Lipids were separated from the water-soluble substances by lipid extraction. Water-soluble substances were analyzed by ESI-MS. A representative experiment is shown.

    Techniques Used: Mass Spectrometry, Incubation

    10) Product Images from "Novel Phospholipase A Activity Secreted by Legionella Species"

    Article Title: Novel Phospholipase A Activity Secreted by Legionella Species

    Journal: Journal of Bacteriology

    doi:

    Apolar and polar lipids after incubation of PC with CCCS of L. pneumophila . PC was incubated with the CCCS of different L. pneumophila strains for 16 h. Apolar (A) and polar (B) lipids were characterized by TLC. A representative experiment is shown. Abbreviations: St, standard (1A, 1,2-dipalmitoylglycerol, palmitic acid; 1B, LPC, dipalmitoylphosphatidylcholine); C, negative control (concentrated BYE broth or concentrated −P broth, respectively); P, positive control (PLC from C. perfringens ); 1, Lp1-MM6; 2, Lp1-MRC; 3, Lp2-ATCC; 4, Lp6-c; 5, Lp12-ATCC.
    Figure Legend Snippet: Apolar and polar lipids after incubation of PC with CCCS of L. pneumophila . PC was incubated with the CCCS of different L. pneumophila strains for 16 h. Apolar (A) and polar (B) lipids were characterized by TLC. A representative experiment is shown. Abbreviations: St, standard (1A, 1,2-dipalmitoylglycerol, palmitic acid; 1B, LPC, dipalmitoylphosphatidylcholine); C, negative control (concentrated BYE broth or concentrated −P broth, respectively); P, positive control (PLC from C. perfringens ); 1, Lp1-MM6; 2, Lp1-MRC; 3, Lp2-ATCC; 4, Lp6-c; 5, Lp12-ATCC.

    Techniques Used: Incubation, Thin Layer Chromatography, Negative Control, Positive Control, Planar Chromatography

    Characterization of AEC fractions. CCCS of Lp6-c was applied onto an AEC column. Fractions eluted by 0.1 to 0.7 M sodium chloride were investigated for protein content, hydrolysis of p -NPPC (OD 410 ), and hydrolysis of phosphomonoesters (P i content). A representative experiment is shown.
    Figure Legend Snippet: Characterization of AEC fractions. CCCS of Lp6-c was applied onto an AEC column. Fractions eluted by 0.1 to 0.7 M sodium chloride were investigated for protein content, hydrolysis of p -NPPC (OD 410 ), and hydrolysis of phosphomonoesters (P i content). A representative experiment is shown.

    Techniques Used:

    ESI-MS. CCCS of Lp6-c was incubated with Alveofact for 16 h. Lipids were separated from the water-soluble substances by lipid extraction. Water-soluble substances were analyzed by ESI-MS. A representative experiment is shown.
    Figure Legend Snippet: ESI-MS. CCCS of Lp6-c was incubated with Alveofact for 16 h. Lipids were separated from the water-soluble substances by lipid extraction. Water-soluble substances were analyzed by ESI-MS. A representative experiment is shown.

    Techniques Used: Mass Spectrometry, Incubation

    11) Product Images from "Novel Phospholipase A Activity Secreted by Legionella Species"

    Article Title: Novel Phospholipase A Activity Secreted by Legionella Species

    Journal: Journal of Bacteriology

    doi:

    Apolar and polar lipids after incubation of PC with CCCS of L. pneumophila . PC was incubated with the CCCS of different L. pneumophila strains for 16 h. Apolar (A) and polar (B) lipids were characterized by TLC. A representative experiment is shown. Abbreviations: St, standard (1A, 1,2-dipalmitoylglycerol, palmitic acid; 1B, LPC, dipalmitoylphosphatidylcholine); C, negative control (concentrated BYE broth or concentrated −P broth, respectively); P, positive control (PLC from C. perfringens ); 1, Lp1-MM6; 2, Lp1-MRC; 3, Lp2-ATCC; 4, Lp6-c; 5, Lp12-ATCC.
    Figure Legend Snippet: Apolar and polar lipids after incubation of PC with CCCS of L. pneumophila . PC was incubated with the CCCS of different L. pneumophila strains for 16 h. Apolar (A) and polar (B) lipids were characterized by TLC. A representative experiment is shown. Abbreviations: St, standard (1A, 1,2-dipalmitoylglycerol, palmitic acid; 1B, LPC, dipalmitoylphosphatidylcholine); C, negative control (concentrated BYE broth or concentrated −P broth, respectively); P, positive control (PLC from C. perfringens ); 1, Lp1-MM6; 2, Lp1-MRC; 3, Lp2-ATCC; 4, Lp6-c; 5, Lp12-ATCC.

    Techniques Used: Incubation, Thin Layer Chromatography, Negative Control, Positive Control, Planar Chromatography

    Characterization of AEC fractions. CCCS of Lp6-c was applied onto an AEC column. Fractions eluted by 0.1 to 0.7 M sodium chloride were investigated for protein content, hydrolysis of p -NPPC (OD 410 ), and hydrolysis of phosphomonoesters (P i content). A representative experiment is shown.
    Figure Legend Snippet: Characterization of AEC fractions. CCCS of Lp6-c was applied onto an AEC column. Fractions eluted by 0.1 to 0.7 M sodium chloride were investigated for protein content, hydrolysis of p -NPPC (OD 410 ), and hydrolysis of phosphomonoesters (P i content). A representative experiment is shown.

    Techniques Used:

    ESI-MS. CCCS of Lp6-c was incubated with Alveofact for 16 h. Lipids were separated from the water-soluble substances by lipid extraction. Water-soluble substances were analyzed by ESI-MS. A representative experiment is shown.
    Figure Legend Snippet: ESI-MS. CCCS of Lp6-c was incubated with Alveofact for 16 h. Lipids were separated from the water-soluble substances by lipid extraction. Water-soluble substances were analyzed by ESI-MS. A representative experiment is shown.

    Techniques Used: Mass Spectrometry, Incubation

    12) Product Images from "Novel Phospholipase A Activity Secreted by Legionella Species"

    Article Title: Novel Phospholipase A Activity Secreted by Legionella Species

    Journal: Journal of Bacteriology

    doi:

    Apolar and polar lipids after incubation of PC with CCCS of L. pneumophila . PC was incubated with the CCCS of different L. pneumophila strains for 16 h. Apolar (A) and polar (B) lipids were characterized by TLC. A representative experiment is shown. Abbreviations: St, standard (1A, 1,2-dipalmitoylglycerol, palmitic acid; 1B, LPC, dipalmitoylphosphatidylcholine); C, negative control (concentrated BYE broth or concentrated −P broth, respectively); P, positive control (PLC from C. perfringens ); 1, Lp1-MM6; 2, Lp1-MRC; 3, Lp2-ATCC; 4, Lp6-c; 5, Lp12-ATCC.
    Figure Legend Snippet: Apolar and polar lipids after incubation of PC with CCCS of L. pneumophila . PC was incubated with the CCCS of different L. pneumophila strains for 16 h. Apolar (A) and polar (B) lipids were characterized by TLC. A representative experiment is shown. Abbreviations: St, standard (1A, 1,2-dipalmitoylglycerol, palmitic acid; 1B, LPC, dipalmitoylphosphatidylcholine); C, negative control (concentrated BYE broth or concentrated −P broth, respectively); P, positive control (PLC from C. perfringens ); 1, Lp1-MM6; 2, Lp1-MRC; 3, Lp2-ATCC; 4, Lp6-c; 5, Lp12-ATCC.

    Techniques Used: Incubation, Thin Layer Chromatography, Negative Control, Positive Control, Planar Chromatography

    Characterization of AEC fractions. CCCS of Lp6-c was applied onto an AEC column. Fractions eluted by 0.1 to 0.7 M sodium chloride were investigated for protein content, hydrolysis of p -NPPC (OD 410 ), and hydrolysis of phosphomonoesters (P i content). A representative experiment is shown.
    Figure Legend Snippet: Characterization of AEC fractions. CCCS of Lp6-c was applied onto an AEC column. Fractions eluted by 0.1 to 0.7 M sodium chloride were investigated for protein content, hydrolysis of p -NPPC (OD 410 ), and hydrolysis of phosphomonoesters (P i content). A representative experiment is shown.

    Techniques Used:

    ESI-MS. CCCS of Lp6-c was incubated with Alveofact for 16 h. Lipids were separated from the water-soluble substances by lipid extraction. Water-soluble substances were analyzed by ESI-MS. A representative experiment is shown.
    Figure Legend Snippet: ESI-MS. CCCS of Lp6-c was incubated with Alveofact for 16 h. Lipids were separated from the water-soluble substances by lipid extraction. Water-soluble substances were analyzed by ESI-MS. A representative experiment is shown.

    Techniques Used: Mass Spectrometry, Incubation

    Related Articles

    Incubation:

    Article Title: Novel Phospholipase A Activity Secreted by Legionella Species
    Article Snippet: Therefore, we determined the release of water-soluble products from Alveofact by CCCS of Lp6-c by using ESI-MS. PrC (signals at 184.0 [PrC-H], 205.9 [PrC-Na], and 221.9 [PrC-K]) could be found after incubation of PLC from C. perfringens with surfactant (data not shown). .. After incubation of CCCS of Lp6-c with Alveofact, only GPC (signals at 258.0 [GPC-H], 279.9 [GPC-Na], and 295.9 [GPC-K]) but not PrC could be detected (Fig. ). .. Since the concentration of inorganic phosphate within the aqueous phase of lipid extraction did not increase after incubation of PLs with CCCS of Lp6-c (data not shown), a possible cleavage of PrC by phosphatases could be excluded.

    Gel Permeation Chromatography:

    Article Title: Novel Phospholipase A Activity Secreted by Legionella Species
    Article Snippet: Therefore, we determined the release of water-soluble products from Alveofact by CCCS of Lp6-c by using ESI-MS. PrC (signals at 184.0 [PrC-H], 205.9 [PrC-Na], and 221.9 [PrC-K]) could be found after incubation of PLC from C. perfringens with surfactant (data not shown). .. After incubation of CCCS of Lp6-c with Alveofact, only GPC (signals at 258.0 [GPC-H], 279.9 [GPC-Na], and 295.9 [GPC-K]) but not PrC could be detected (Fig. ). .. Since the concentration of inorganic phosphate within the aqueous phase of lipid extraction did not increase after incubation of PLs with CCCS of Lp6-c (data not shown), a possible cleavage of PrC by phosphatases could be excluded.

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    Apolar and polar lipids after incubation of PC with CCCS of L. pneumophila . PC was incubated with the CCCS of different L. pneumophila strains for 16 h. Apolar (A) and polar (B) lipids were characterized by TLC. A representative experiment is shown. Abbreviations: St, standard (1A, 1,2-dipalmitoylglycerol, palmitic acid; 1B, LPC, dipalmitoylphosphatidylcholine); C, negative control (concentrated BYE broth or concentrated −P broth, respectively); P, positive control (PLC from C. perfringens ); 1, Lp1-MM6; 2, Lp1-MRC; 3, Lp2-ATCC; 4, <t>Lp6-c;</t> 5, Lp12-ATCC.
    Lp6 C, supplied by ATCC, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Apolar and polar lipids after incubation of PC with CCCS of L. pneumophila . PC was incubated with the CCCS of different L. pneumophila strains for 16 h. Apolar (A) and polar (B) lipids were characterized by TLC. A representative experiment is shown. Abbreviations: St, standard (1A, 1,2-dipalmitoylglycerol, palmitic acid; 1B, LPC, dipalmitoylphosphatidylcholine); C, negative control (concentrated BYE broth or concentrated −P broth, respectively); P, positive control (PLC from C. perfringens ); 1, Lp1-MM6; 2, Lp1-MRC; 3, Lp2-ATCC; 4, Lp6-c; 5, Lp12-ATCC.

    Journal: Journal of Bacteriology

    Article Title: Novel Phospholipase A Activity Secreted by Legionella Species

    doi:

    Figure Lengend Snippet: Apolar and polar lipids after incubation of PC with CCCS of L. pneumophila . PC was incubated with the CCCS of different L. pneumophila strains for 16 h. Apolar (A) and polar (B) lipids were characterized by TLC. A representative experiment is shown. Abbreviations: St, standard (1A, 1,2-dipalmitoylglycerol, palmitic acid; 1B, LPC, dipalmitoylphosphatidylcholine); C, negative control (concentrated BYE broth or concentrated −P broth, respectively); P, positive control (PLC from C. perfringens ); 1, Lp1-MM6; 2, Lp1-MRC; 3, Lp2-ATCC; 4, Lp6-c; 5, Lp12-ATCC.

    Article Snippet: After incubation of CCCS of Lp6-c with Alveofact, only GPC (signals at 258.0 [GPC-H], 279.9 [GPC-Na], and 295.9 [GPC-K]) but not PrC could be detected (Fig. ).

    Techniques: Incubation, Thin Layer Chromatography, Negative Control, Positive Control, Planar Chromatography

    Characterization of AEC fractions. CCCS of Lp6-c was applied onto an AEC column. Fractions eluted by 0.1 to 0.7 M sodium chloride were investigated for protein content, hydrolysis of p -NPPC (OD 410 ), and hydrolysis of phosphomonoesters (P i content). A representative experiment is shown.

    Journal: Journal of Bacteriology

    Article Title: Novel Phospholipase A Activity Secreted by Legionella Species

    doi:

    Figure Lengend Snippet: Characterization of AEC fractions. CCCS of Lp6-c was applied onto an AEC column. Fractions eluted by 0.1 to 0.7 M sodium chloride were investigated for protein content, hydrolysis of p -NPPC (OD 410 ), and hydrolysis of phosphomonoesters (P i content). A representative experiment is shown.

    Article Snippet: After incubation of CCCS of Lp6-c with Alveofact, only GPC (signals at 258.0 [GPC-H], 279.9 [GPC-Na], and 295.9 [GPC-K]) but not PrC could be detected (Fig. ).

    Techniques:

    ESI-MS. CCCS of Lp6-c was incubated with Alveofact for 16 h. Lipids were separated from the water-soluble substances by lipid extraction. Water-soluble substances were analyzed by ESI-MS. A representative experiment is shown.

    Journal: Journal of Bacteriology

    Article Title: Novel Phospholipase A Activity Secreted by Legionella Species

    doi:

    Figure Lengend Snippet: ESI-MS. CCCS of Lp6-c was incubated with Alveofact for 16 h. Lipids were separated from the water-soluble substances by lipid extraction. Water-soluble substances were analyzed by ESI-MS. A representative experiment is shown.

    Article Snippet: After incubation of CCCS of Lp6-c with Alveofact, only GPC (signals at 258.0 [GPC-H], 279.9 [GPC-Na], and 295.9 [GPC-K]) but not PrC could be detected (Fig. ).

    Techniques: Mass Spectrometry, Incubation