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Pacific Biosciences low input dna
Low Input Dna, supplied by Pacific Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/low input dna/product/Pacific Biosciences
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
low input dna - by Bioz Stars, 2021-07
86/100 stars

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Ligation:

Article Title: The central exons of the human MUC2 and MUC6 mucins are highly repetitive and variable in sequence between individuals
Article Snippet: SMRT sequencing was performed on the PacBio RS II according to standard protocol using MagBead loading with P4-C2 chemistry and a 120 min movie. .. SMRT bell DNA template library of the Hinf I MUC2 PTS-TR2 fragment (NG-7351) (3.2 µg) was performed without further fragmentation, through direct adaptor ligation using a standard protocol for 10 kb template preparation and sequencing with low-input DNA (Pacific Biosciences). .. SMRT sequencing was performed as described above.

Article Title: The central exons of the human MUC2 and MUC6 mucins are highly repetitive and variable in sequence between individuals
Article Snippet: SMRT sequencing was performed on the PacBio RS II according to standard protocol using MagBead loading with P4-C2 chemistry and a 120 min movie. .. SMRT bell DNA template library of the Hinf I MUC2 PTS-TR2 fragment (NG-7351) (3.2 µg) was performed without further fragmentation, through direct adaptor ligation using a standard protocol for 10 kb template preparation and sequencing with low-input DNA (Pacific Biosciences). .. SMRT sequencing was performed as described above.

Sequencing:

Article Title: The central exons of the human MUC2 and MUC6 mucins are highly repetitive and variable in sequence between individuals
Article Snippet: SMRT sequencing was performed on the PacBio RS II according to standard protocol using MagBead loading with P4-C2 chemistry and a 120 min movie. .. SMRT bell DNA template library of the Hinf I MUC2 PTS-TR2 fragment (NG-7351) (3.2 µg) was performed without further fragmentation, through direct adaptor ligation using a standard protocol for 10 kb template preparation and sequencing with low-input DNA (Pacific Biosciences). .. SMRT sequencing was performed as described above.

Article Title: The central exons of the human MUC2 and MUC6 mucins are highly repetitive and variable in sequence between individuals
Article Snippet: SMRT sequencing was performed on the PacBio RS II according to standard protocol using MagBead loading with P4-C2 chemistry and a 120 min movie. .. SMRT bell DNA template library of the Hinf I MUC2 PTS-TR2 fragment (NG-7351) (3.2 µg) was performed without further fragmentation, through direct adaptor ligation using a standard protocol for 10 kb template preparation and sequencing with low-input DNA (Pacific Biosciences). .. SMRT sequencing was performed as described above.

Article Title: Two high-quality de novo genomes from single ethanol-preserved specimens of tiny metazoans (Collembola)
Article Snippet: .. We extracted DNA from single specimens, preserved for 3–45 days in 96% ethanol, and used a recent whole-genome amplification-based ultra-low DNA input workflow for Single Molecule, Real-Time (SMRT) Sequencing (PacBio) [ ] to produce libraries from as little as 5 ng DNA input. ..

Article Title: The central exons of the human MUC2 and MUC6 mucins are highly repetitive and variable in sequence between individuals
Article Snippet: BAC DNA (NG-6867) (10 µg) was fragmented with G-tube (Covaris) and a SMRT bell DNA template library with 8–12 kb insert size was prepared according to standard protocol (Pacific Biosciences) followed by size selection using AMPure PB magnetic beads. .. SMRT sequencing was performed on the PacBio RS II according to standard protocol using MagBead loading with P4-C2 chemistry and a 120 min movie. .. SMRT bell DNA template library of the Hinf I MUC2 PTS-TR2 fragment (NG-7351) (3.2 µg) was performed without further fragmentation, through direct adaptor ligation using a standard protocol for 10 kb template preparation and sequencing with low-input DNA (Pacific Biosciences).

Article Title: Re-sequencing transgenic plants revealed rearrangements at T-DNA inserts, and integration of a short T-DNA fragment, but no increase of small mutations elsewhere
Article Snippet: .. In addition, DNA of transgenic plants At2 and At5 was used for PacBio SMRTbell library preparation according to the manufacture’s protocol (10 kb Template Preparation and Sequencing with Low-input DNA, Pacific Biosciences). .. Final SMRTbells were size selected on a 0.75% agarose gel using a Blue pippin device (Sage sciences) with 5 Kb as cutoff for minimal fragment size.

Article Title: The central exons of the human MUC2 and MUC6 mucins are highly repetitive and variable in sequence between individuals
Article Snippet: SMRT sequencing and Bioinformatics BAC DNA (NG-6867) (10 µg) was fragmented with G-tube (Covaris) and a SMRT bell DNA template library with 8–12 kb insert size was prepared according to standard protocol (Pacific Biosciences) followed by size selection using AMPure PB magnetic beads. .. SMRT sequencing was performed on the PacBio RS II according to standard protocol using MagBead loading with P4-C2 chemistry and a 120 min movie. .. SMRT bell DNA template library of the Hinf I MUC2 PTS-TR2 fragment (NG-7351) (3.2 µg) was performed without further fragmentation, through direct adaptor ligation using a standard protocol for 10 kb template preparation and sequencing with low-input DNA (Pacific Biosciences).

Article Title: Two high-quality de novo genomes from single ethanol-preserved specimens of tiny metazoans (Collembola)
Article Snippet: .. Libraries were prepared using an early access kit for the Ultra-Low DNA Input Workflow for SMRT Sequencing (PacBio) [ ]. .. Of the genomic hmw DNA extracts, 5 ng was fragmented with g-Tubes (Covaris) in an Eppendorf 5424 R centrifuge for 2 min at 1,902g.

Amplification:

Article Title: Two high-quality de novo genomes from single ethanol-preserved specimens of tiny metazoans (Collembola)
Article Snippet: .. We extracted DNA from single specimens, preserved for 3–45 days in 96% ethanol, and used a recent whole-genome amplification-based ultra-low DNA input workflow for Single Molecule, Real-Time (SMRT) Sequencing (PacBio) [ ] to produce libraries from as little as 5 ng DNA input. ..

Transgenic Assay:

Article Title: Re-sequencing transgenic plants revealed rearrangements at T-DNA inserts, and integration of a short T-DNA fragment, but no increase of small mutations elsewhere
Article Snippet: .. In addition, DNA of transgenic plants At2 and At5 was used for PacBio SMRTbell library preparation according to the manufacture’s protocol (10 kb Template Preparation and Sequencing with Low-input DNA, Pacific Biosciences). .. Final SMRTbells were size selected on a 0.75% agarose gel using a Blue pippin device (Sage sciences) with 5 Kb as cutoff for minimal fragment size.

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    Pacific Biosciences pacbio low input workflow
    Flow chart of DNA extraction and ultra-low input <t>workflow</t> for SMRTbell Express 2.0 library preparation, for a single ethanol-preserved specimen. gDNA: genomic DNA; QC: quality control.
    Pacbio Low Input Workflow, supplied by Pacific Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pacbio low input workflow/product/Pacific Biosciences
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pacbio low input workflow - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

    86
    Pacific Biosciences low input 10 kbp protocol
    Flow chart of DNA extraction and ultra-low input <t>workflow</t> for SMRTbell Express 2.0 library preparation, for a single ethanol-preserved specimen. gDNA: genomic DNA; QC: quality control.
    Low Input 10 Kbp Protocol, supplied by Pacific Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/low input 10 kbp protocol/product/Pacific Biosciences
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    low input 10 kbp protocol - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

    86
    Pacific Biosciences low input dna protocol
    Flow chart of DNA extraction and ultra-low input <t>workflow</t> for SMRTbell Express 2.0 library preparation, for a single ethanol-preserved specimen. gDNA: genomic DNA; QC: quality control.
    Low Input Dna Protocol, supplied by Pacific Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/low input dna protocol/product/Pacific Biosciences
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    low input dna protocol - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

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    Flow chart of DNA extraction and ultra-low input workflow for SMRTbell Express 2.0 library preparation, for a single ethanol-preserved specimen. gDNA: genomic DNA; QC: quality control.

    Journal: GigaScience

    Article Title: Two high-quality de novo genomes from single ethanol-preserved specimens of tiny metazoans (Collembola)

    doi: 10.1093/gigascience/giab035

    Figure Lengend Snippet: Flow chart of DNA extraction and ultra-low input workflow for SMRTbell Express 2.0 library preparation, for a single ethanol-preserved specimen. gDNA: genomic DNA; QC: quality control.

    Article Snippet: The 5 ng input of the PacBio ultra-low input workflow is a significant decrease from the 150 ng input required by the PacBio low-input workflow (whole-genome amplification [WGA]-free).

    Techniques: DNA Extraction