Structured Review

Eiken Chemical loopamp realtime turbidimeter
Comparison of the sensitivities of LAMP and conventional PCR assays for detecting sul2 in S. maltophilia . (A) Turbidity was monitored every 6 s using a <t>Loopamp</t> <t>Realtime</t> Turbidimeter with detection at 650 nm. The reaction was performed at 65°C. (B) The results were visualized by adding HNB to the reaction mixture before the reaction. (C) PCR products were separated by 1% (w/v) agarose gel electrophoresis and stained with ethidium bromide. (1) 26.0 ng; (2) 2.6 ng; (3) 0.26 ng; (4) 26.0 pg; (5) 2.6 pg; (6) 0.26 pg; (7) 26.0 fg; (8) blank control (double-distilled water).
Loopamp Realtime Turbidimeter, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 90/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/loopamp realtime turbidimeter/product/Eiken Chemical
Average 90 stars, based on 16 article reviews
Price from $9.99 to $1999.99
loopamp realtime turbidimeter - by Bioz Stars, 2020-08
90/100 stars

Images

1) Product Images from "Surveillance of Dihydropteroate Synthase Genes in Stenotrophomonas maltophilia by LAMP: Implications for Infection Control and Initial Therapy"

Article Title: Surveillance of Dihydropteroate Synthase Genes in Stenotrophomonas maltophilia by LAMP: Implications for Infection Control and Initial Therapy

Journal: Frontiers in Microbiology

doi: 10.3389/fmicb.2016.01723

Comparison of the sensitivities of LAMP and conventional PCR assays for detecting sul2 in S. maltophilia . (A) Turbidity was monitored every 6 s using a Loopamp Realtime Turbidimeter with detection at 650 nm. The reaction was performed at 65°C. (B) The results were visualized by adding HNB to the reaction mixture before the reaction. (C) PCR products were separated by 1% (w/v) agarose gel electrophoresis and stained with ethidium bromide. (1) 26.0 ng; (2) 2.6 ng; (3) 0.26 ng; (4) 26.0 pg; (5) 2.6 pg; (6) 0.26 pg; (7) 26.0 fg; (8) blank control (double-distilled water).
Figure Legend Snippet: Comparison of the sensitivities of LAMP and conventional PCR assays for detecting sul2 in S. maltophilia . (A) Turbidity was monitored every 6 s using a Loopamp Realtime Turbidimeter with detection at 650 nm. The reaction was performed at 65°C. (B) The results were visualized by adding HNB to the reaction mixture before the reaction. (C) PCR products were separated by 1% (w/v) agarose gel electrophoresis and stained with ethidium bromide. (1) 26.0 ng; (2) 2.6 ng; (3) 0.26 ng; (4) 26.0 pg; (5) 2.6 pg; (6) 0.26 pg; (7) 26.0 fg; (8) blank control (double-distilled water).

Techniques Used: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Staining

Comparison of the sensitivities of LAMP and conventional PCR assays for detecting sul1 in Stenotrophomonas maltophilia . (A) Turbidity was monitored every 6 s using a Loopamp Realtime Turbidimeter with detection at 650 nm. The reaction was performed at 65°C. (B) The results were visualized by adding hydroxy naphthol blue (HNB) to the reaction mixture before the reaction. (C) PCR products were separated by 1% (w/v) agarose gel electrophoresis and stained with ethidium bromide. (1) 74.0 ng; (2) 7.4 ng; (3) 0.74 ng; (4) 74.0 pg; (5) 7.4 pg; (6) 0.74 pg; (7) 74.0 fg; (8) 7.4 fg; (9) blank control (double-distilled water).
Figure Legend Snippet: Comparison of the sensitivities of LAMP and conventional PCR assays for detecting sul1 in Stenotrophomonas maltophilia . (A) Turbidity was monitored every 6 s using a Loopamp Realtime Turbidimeter with detection at 650 nm. The reaction was performed at 65°C. (B) The results were visualized by adding hydroxy naphthol blue (HNB) to the reaction mixture before the reaction. (C) PCR products were separated by 1% (w/v) agarose gel electrophoresis and stained with ethidium bromide. (1) 74.0 ng; (2) 7.4 ng; (3) 0.74 ng; (4) 74.0 pg; (5) 7.4 pg; (6) 0.74 pg; (7) 74.0 fg; (8) 7.4 fg; (9) blank control (double-distilled water).

Techniques Used: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Staining

Specificity of the LAMP reaction for detecting sul1 in S. maltophilia . (A) Turbidity was monitored every 6 s using a Loopamp Realtime Turbidimeter with detection at 650 nm. The reaction was performed at 65°C. (B) The results were visualized by adding HNB to the reaction mixture before the reaction. (1) X2 (positive control for sul1 from Peking Union Medical College Hospital); (2) P121 (positive control for sul1 from Chinese PLA General Hospital); (3) K106 (positive control for sul1 from Chinese PLA General Hospital); (4) S. maltophilia K279a; (5) S. maltophilia ATCC 13637; (6) P129 (negative control for sul from Chinese PLA General Hospital, confirmed by genome sequencing); (7) double-distilled water.
Figure Legend Snippet: Specificity of the LAMP reaction for detecting sul1 in S. maltophilia . (A) Turbidity was monitored every 6 s using a Loopamp Realtime Turbidimeter with detection at 650 nm. The reaction was performed at 65°C. (B) The results were visualized by adding HNB to the reaction mixture before the reaction. (1) X2 (positive control for sul1 from Peking Union Medical College Hospital); (2) P121 (positive control for sul1 from Chinese PLA General Hospital); (3) K106 (positive control for sul1 from Chinese PLA General Hospital); (4) S. maltophilia K279a; (5) S. maltophilia ATCC 13637; (6) P129 (negative control for sul from Chinese PLA General Hospital, confirmed by genome sequencing); (7) double-distilled water.

Techniques Used: Positive Control, Proximity Ligation Assay, Negative Control, Sequencing

Specificity of the LAMP reaction for detecting sul2 in S. maltophilia. (A) Turbidity was monitored every 6 s using a Loopamp Realtime Turbidimeter with detection at 650 nm. The reaction was performed at 65°C. (B) The results were visualized by adding HNB to the reaction mixture before the reaction. (1) X133 (positive control for sul2 from Peking Union Medical College Hospital); (2) P57 (positive control for sul2 from Chinese PLA General Hospital); (3) K14 (positive control for sul2 from Chinese PLA General Hospital); (4) S. maltophilia K279a; (5) S. maltophilia ATCC 13637; (6) P129 (negative control for sul from Chinese PLA General Hospital, confirmed by genome sequencing); (7) double-distilled water.
Figure Legend Snippet: Specificity of the LAMP reaction for detecting sul2 in S. maltophilia. (A) Turbidity was monitored every 6 s using a Loopamp Realtime Turbidimeter with detection at 650 nm. The reaction was performed at 65°C. (B) The results were visualized by adding HNB to the reaction mixture before the reaction. (1) X133 (positive control for sul2 from Peking Union Medical College Hospital); (2) P57 (positive control for sul2 from Chinese PLA General Hospital); (3) K14 (positive control for sul2 from Chinese PLA General Hospital); (4) S. maltophilia K279a; (5) S. maltophilia ATCC 13637; (6) P129 (negative control for sul from Chinese PLA General Hospital, confirmed by genome sequencing); (7) double-distilled water.

Techniques Used: Positive Control, Proximity Ligation Assay, Negative Control, Sequencing

2) Product Images from "Survey and rapid detection of Klebsiella pneumoniae in clinical samples targeting the rcsA gene in Beijing, China"

Article Title: Survey and rapid detection of Klebsiella pneumoniae in clinical samples targeting the rcsA gene in Beijing, China

Journal: Frontiers in Microbiology

doi: 10.3389/fmicb.2015.00519

Different temperatures at which the LAMP reaction detected K. pneumoniae . Turbidity was monitored every 6 s with a Loopamp Realtime Turbidimeter at 650 nm.
Figure Legend Snippet: Different temperatures at which the LAMP reaction detected K. pneumoniae . Turbidity was monitored every 6 s with a Loopamp Realtime Turbidimeter at 650 nm.

Techniques Used:

LAMP results for K. pneumoniae isolates from clinical samples. (A) Turbidity was monitored every 6 s using a Loopamp Realtime Turbidimeter at 650 nm. (B) The results were visualized by the addition of 1 μl of fluorescent detection reagent to the 25 μl LAMP reaction mixture before the LAMP reaction. 1, Negative control (double-distilled water); 2, positive control ( K. pneumoniae ATCC BAA-2146); 3, K. pneumoniae WJ-48; 4, K. pneumoniae WJ-50; 5, K. pneumoniae WJ-51; 6, K. pneumoniae WJ-52; 7, K. pneumoniae WJ-53; 8, K. pneumoniae WJ-57; 9, K. pneumoniae WJ-58; 10, K. pneumoniae WJ-60; 11, K. pneumoniae WJ-61; 12, K. pneumoniae WJ-64; 13, K. pneumoniae WJ-65; 14, K. pneumoniae WJ-66; 15, K. pneumoniae WJ-68; 16, K. pneumoniae 301-052; 17, K. pneumoniae 301-207; 18, K. pneumoniae 301-263; 19, K. pneumoniae 301-432; 20, K. pneumoniae 301-416; 21, K. pneumoniae 301-323; 22, K. pneumoniae 301-365; 23, K. pneumoniae 301-282; 24, K. pneumoniae 301-406; 25, K. pneumoniae 301-158; 26, K. pneumoniae 307-206; 27, K. pneumoniae 307-082; 28, K. pneumoniae 307-429; 29, K. pneumoniae 307-095; 30, K. pneumoniae 307-003; 31, K. pneumoniae 307-030; 32, K. pneumoniae 307-194; 33, K. pneumoniae 307-356; 34, K. pneumoniae 307-235.
Figure Legend Snippet: LAMP results for K. pneumoniae isolates from clinical samples. (A) Turbidity was monitored every 6 s using a Loopamp Realtime Turbidimeter at 650 nm. (B) The results were visualized by the addition of 1 μl of fluorescent detection reagent to the 25 μl LAMP reaction mixture before the LAMP reaction. 1, Negative control (double-distilled water); 2, positive control ( K. pneumoniae ATCC BAA-2146); 3, K. pneumoniae WJ-48; 4, K. pneumoniae WJ-50; 5, K. pneumoniae WJ-51; 6, K. pneumoniae WJ-52; 7, K. pneumoniae WJ-53; 8, K. pneumoniae WJ-57; 9, K. pneumoniae WJ-58; 10, K. pneumoniae WJ-60; 11, K. pneumoniae WJ-61; 12, K. pneumoniae WJ-64; 13, K. pneumoniae WJ-65; 14, K. pneumoniae WJ-66; 15, K. pneumoniae WJ-68; 16, K. pneumoniae 301-052; 17, K. pneumoniae 301-207; 18, K. pneumoniae 301-263; 19, K. pneumoniae 301-432; 20, K. pneumoniae 301-416; 21, K. pneumoniae 301-323; 22, K. pneumoniae 301-365; 23, K. pneumoniae 301-282; 24, K. pneumoniae 301-406; 25, K. pneumoniae 301-158; 26, K. pneumoniae 307-206; 27, K. pneumoniae 307-082; 28, K. pneumoniae 307-429; 29, K. pneumoniae 307-095; 30, K. pneumoniae 307-003; 31, K. pneumoniae 307-030; 32, K. pneumoniae 307-194; 33, K. pneumoniae 307-356; 34, K. pneumoniae 307-235.

Techniques Used: Negative Control, Positive Control

Five sets of primers were used to amplify the target gene under the same conditions . Turbidity was monitored every 6 s with a Loopamp Realtime Turbidimeter at 650 nm.
Figure Legend Snippet: Five sets of primers were used to amplify the target gene under the same conditions . Turbidity was monitored every 6 s with a Loopamp Realtime Turbidimeter at 650 nm.

Techniques Used:

Specificity of the LAMP reaction in detecting K. pneumoniae . (A) Turbidity was monitored every 6 s using a Loopamp Realtime Turbidimeter at 650 nm. (B) The results were visualized by the addition of 1 μl of fluorescent detection reagent to the 25 μl LAMP reaction mixture before the LAMP reaction. Amplification was performed at 61°C for 60 min. 1, positive control ( K. pneumoniae ATCC BAA-2146); 2, negative control (double-distilled water); 3, Klebsiella oxytoca ATCC 700324; 4, Klebsiella rhinoscleromatis CMCC 46111; 5, Citrobacter freundii CMCC 48001; 6, Enterobacter aerogenes ATCC 13048; 7, Enterobacter cloacae ATCC 13047; 8, Proteus mirabilis CMCC 49005; 9, Proteus vulgaris CMCC 49027; 10, Serratia marcescens ATCC 14756; 11, Morganella morganii ATCC 25830; 12, Streptococcus pneumoniae 112-07; 13, Mycobacterium tuberculosis 005; 14, Pseudomonas aeruginosa D104; 15, Haemophilus influenza ATCC 49247; 16, Yersinia enterocolitica 027; 17, Yersinia pestis 2638; 18, Bacillus tularense 3450; 19, Vibrio cholera 3802; 20, Salmonella aberdeen 9264; 21, Neisseria meningitides CMCC 29022; 22, Staphylococcus aureus 2740; 23, Pseudomonas pseudomallei 029; 24, Salmonella typhimurium 4030; 25, Corynebacterium diphtheriae CMCC 38001; 26, Bacillus megatherium 4623; 27, Stenotrophomonas maltophilia K279a; 28, Legionella pneumophila 9135; 29, Acinetobacter baumannii 12101; 30, enteroinvasive E. coli 44825; 31, enterotoxigenic E. coli 44824; 32, enteropathogenic E. coli 2348.
Figure Legend Snippet: Specificity of the LAMP reaction in detecting K. pneumoniae . (A) Turbidity was monitored every 6 s using a Loopamp Realtime Turbidimeter at 650 nm. (B) The results were visualized by the addition of 1 μl of fluorescent detection reagent to the 25 μl LAMP reaction mixture before the LAMP reaction. Amplification was performed at 61°C for 60 min. 1, positive control ( K. pneumoniae ATCC BAA-2146); 2, negative control (double-distilled water); 3, Klebsiella oxytoca ATCC 700324; 4, Klebsiella rhinoscleromatis CMCC 46111; 5, Citrobacter freundii CMCC 48001; 6, Enterobacter aerogenes ATCC 13048; 7, Enterobacter cloacae ATCC 13047; 8, Proteus mirabilis CMCC 49005; 9, Proteus vulgaris CMCC 49027; 10, Serratia marcescens ATCC 14756; 11, Morganella morganii ATCC 25830; 12, Streptococcus pneumoniae 112-07; 13, Mycobacterium tuberculosis 005; 14, Pseudomonas aeruginosa D104; 15, Haemophilus influenza ATCC 49247; 16, Yersinia enterocolitica 027; 17, Yersinia pestis 2638; 18, Bacillus tularense 3450; 19, Vibrio cholera 3802; 20, Salmonella aberdeen 9264; 21, Neisseria meningitides CMCC 29022; 22, Staphylococcus aureus 2740; 23, Pseudomonas pseudomallei 029; 24, Salmonella typhimurium 4030; 25, Corynebacterium diphtheriae CMCC 38001; 26, Bacillus megatherium 4623; 27, Stenotrophomonas maltophilia K279a; 28, Legionella pneumophila 9135; 29, Acinetobacter baumannii 12101; 30, enteroinvasive E. coli 44825; 31, enterotoxigenic E. coli 44824; 32, enteropathogenic E. coli 2348.

Techniques Used: Amplification, Positive Control, Negative Control

Comparison of the sensitivities of the LAMP reaction and conventional PCR in detecting the rcsA gene of K. pneumoniae . Pure genomic DNA extracted from K. pneumoniae ATCC BAA-2146 was serially diluted 10-fold. (A) Turbidity was monitored every 6 s with a Loopamp Realtime Turbidimeter at 650 nm. (B) The reaction result was detected visually by the addition of 1 μl of fluorescent detection reagent to the 25 μl LAMP reaction mixture before the LAMP reaction. (C) PCR products were separated by 2% agarose gel electrophoresis and stained with ethidium bromide. Amplification was performed at 61°C for 60 min. 1, negative control (double-distilled water); 2, 115.0 ng/μl; 3, 11.5 ng/μl; 4, 1.15 ng/μl; 5, 115.0 pg/μl; 6, 11.5 pg/μl; 7, 1.15 pg/μl; 8, 0.115 pg/μl; 9, 0.0115 pg/μl.
Figure Legend Snippet: Comparison of the sensitivities of the LAMP reaction and conventional PCR in detecting the rcsA gene of K. pneumoniae . Pure genomic DNA extracted from K. pneumoniae ATCC BAA-2146 was serially diluted 10-fold. (A) Turbidity was monitored every 6 s with a Loopamp Realtime Turbidimeter at 650 nm. (B) The reaction result was detected visually by the addition of 1 μl of fluorescent detection reagent to the 25 μl LAMP reaction mixture before the LAMP reaction. (C) PCR products were separated by 2% agarose gel electrophoresis and stained with ethidium bromide. Amplification was performed at 61°C for 60 min. 1, negative control (double-distilled water); 2, 115.0 ng/μl; 3, 11.5 ng/μl; 4, 1.15 ng/μl; 5, 115.0 pg/μl; 6, 11.5 pg/μl; 7, 1.15 pg/μl; 8, 0.115 pg/μl; 9, 0.0115 pg/μl.

Techniques Used: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Staining, Amplification, Negative Control

Related Articles

Incubation:

Article Title: Loop-mediated isothermal amplification applied to filarial parasites detection in the mosquito vectors: Dirofilaria immitis as a study model
Article Snippet: .. The reaction mixture was incubated at 63°C for 70 min using a Loopamp Realtime Turbidimeter (LA-200; Eiken Chemical Co., Ltd., Tokyo, Japan) and terminated by incubation at 95°C for 2 minutes. .. Analysis of LAMP products Amplified DNA in the LAMP reaction causes turbidity due to the accumulation of magnesium pyrophosphate, a by-product of the reaction.

Article Title: Diagnosis of Brugian Filariasis by Loop-Mediated Isothermal Amplification
Article Snippet: .. Reactions were incubated at 63°C for 60–90 minutes in a Loopamp Realtime Turbidimeter (LA-320c, Eiken Chemical Co.). ..

Article Title: Genome Filtering for New DNA Biomarkers of Loa loa Infection Suitable for Loop-Mediated Isothermal Amplification
Article Snippet: .. Reactions were incubated at 61°C for 60–90 minutes in a Loopamp Realtime Turbidimeter (LA-320c, Eiken Chemical Co.). .. The instrument measures the change in turbidity at 650 nm caused by the precipitation of magnesium pyrophosphate produced by polymerase activity.

Amplification:

Article Title: Multifuntional Gold Nanoparticles for the SERS Detection of Pathogens Combined with a LAMP–in–Microdroplets Approach
Article Snippet: .. The amplification was performed in a Loopamp Realtime Turbidimeter (LA-500, Eiken Chemical Co., Ltd., Tokyo, Japan) at 62 °C for 60 min. DNA extracted from L. monocytogenes WDCM 00021 was selected as positive control. .. Its concentration was determined with a NanoDrop 2000c (Thermo Fisher Scientific Inc., Waltham, MA, USA).

Article Title: Development and Application of Loop-Mediated Isothermal Amplification Assays for Rapid Visual Detection of cry2Ab and cry3A Genes in Genetically-Modified Crops
Article Snippet: .. Analysis of LAMP Products The white turbidity caused by the existence of magnesium pyrophosphate, the LAMP amplification by-product of each reaction mixture, was simultaneously and continuously monitored by using a Loopamp Realtime turbidimeter, LA-320C (Eiken Chemical Co., Ltd., Tochigi, Japan). .. The LAMP-positive reaction mixture became turbid, while the negative reaction without amplified products still remained clear.

other:

Article Title: Loop-mediated isothermal amplification applied to filarial parasites detection in the mosquito vectors: Dirofilaria immitis as a study model
Article Snippet: Turbidity was monitored using Loopamp Realtime Turbidimeter in addition to the naked eye.

Positive Control:

Article Title: Multifuntional Gold Nanoparticles for the SERS Detection of Pathogens Combined with a LAMP–in–Microdroplets Approach
Article Snippet: .. The amplification was performed in a Loopamp Realtime Turbidimeter (LA-500, Eiken Chemical Co., Ltd., Tokyo, Japan) at 62 °C for 60 min. DNA extracted from L. monocytogenes WDCM 00021 was selected as positive control. .. Its concentration was determined with a NanoDrop 2000c (Thermo Fisher Scientific Inc., Waltham, MA, USA).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Eiken Chemical loopamp realtime turbidimeter la 320c
    LAMP assay for A. baumannii amplification of the recA gene using a <t>Loopamp</t> <t>Realtime</t> Turbidimeter <t>LA-320c.</t> ( A ) A. baumannii , amplification curve observed within 36 min of amplification. ( B ) P. aeruginosa, S. maltophilia, S. aureus and P. mirabilis , no amplification curve observed with recA gene amplification.
    Loopamp Realtime Turbidimeter La 320c, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/loopamp realtime turbidimeter la 320c/product/Eiken Chemical
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    loopamp realtime turbidimeter la 320c - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    90
    Eiken Chemical loopamp realtime turbidimeter
    Comparison of the sensitivities of LAMP and conventional PCR assays for detecting sul2 in S. maltophilia . (A) Turbidity was monitored every 6 s using a <t>Loopamp</t> <t>Realtime</t> Turbidimeter with detection at 650 nm. The reaction was performed at 65°C. (B) The results were visualized by adding HNB to the reaction mixture before the reaction. (C) PCR products were separated by 1% (w/v) agarose gel electrophoresis and stained with ethidium bromide. (1) 26.0 ng; (2) 2.6 ng; (3) 0.26 ng; (4) 26.0 pg; (5) 2.6 pg; (6) 0.26 pg; (7) 26.0 fg; (8) blank control (double-distilled water).
    Loopamp Realtime Turbidimeter, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 90/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/loopamp realtime turbidimeter/product/Eiken Chemical
    Average 90 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    loopamp realtime turbidimeter - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    Image Search Results


    LAMP assay for A. baumannii amplification of the recA gene using a Loopamp Realtime Turbidimeter LA-320c. ( A ) A. baumannii , amplification curve observed within 36 min of amplification. ( B ) P. aeruginosa, S. maltophilia, S. aureus and P. mirabilis , no amplification curve observed with recA gene amplification.

    Journal: Bioscience Reports

    Article Title: A multiplex loop-mediated isothermal amplification assay for rapid screening of Acinetobacter baumannii and D carbapenemase OXA-23 gene

    doi: 10.1042/BSR20180425

    Figure Lengend Snippet: LAMP assay for A. baumannii amplification of the recA gene using a Loopamp Realtime Turbidimeter LA-320c. ( A ) A. baumannii , amplification curve observed within 36 min of amplification. ( B ) P. aeruginosa, S. maltophilia, S. aureus and P. mirabilis , no amplification curve observed with recA gene amplification.

    Article Snippet: Three different instruments that allow isothermal amplification of DNA and RNA, Genie II (OptiGene Ltd, United Kingdom) and ROCHE LightCycler 480 (Roche Diagnostics Ltd., Rotkreuz, Switzerland) with target detection using a fluorescent measurement, and Loopamp Realtime Turbidimeter LA-320c (Eiken Chemical Co. Ltd, Tokyo, Japan) with target detection using a turbidimetric assay, were used according to the manufacturer’s instructions.

    Techniques: Lamp Assay, Amplification

    LAMP assay for A. baumannii amplification of the recA gene using a Loopamp Realtime Turbidimeter LA-320c. ( A ) A. baumannii , amplification curve observed within 36 min of amplification. ( B ) P. aeruginosa, S. maltophilia, S. aureus and P. mirabilis , no amplification curve observed with recA gene amplification.

    Journal: Bioscience Reports

    Article Title: A multiplex loop-mediated isothermal amplification assay for rapid screening of Acinetobacter baumannii and D carbapenemase OXA-23 gene

    doi: 10.1042/BSR20180425

    Figure Lengend Snippet: LAMP assay for A. baumannii amplification of the recA gene using a Loopamp Realtime Turbidimeter LA-320c. ( A ) A. baumannii , amplification curve observed within 36 min of amplification. ( B ) P. aeruginosa, S. maltophilia, S. aureus and P. mirabilis , no amplification curve observed with recA gene amplification.

    Article Snippet: Amplifications were carried out using a Loopamp DNA Amplification Kit on Loopamp Realtime Turbidimeter LA-320c.

    Techniques: Lamp Assay, Amplification

    Comparison of the sensitivities of LAMP and conventional PCR assays for detecting sul2 in S. maltophilia . (A) Turbidity was monitored every 6 s using a Loopamp Realtime Turbidimeter with detection at 650 nm. The reaction was performed at 65°C. (B) The results were visualized by adding HNB to the reaction mixture before the reaction. (C) PCR products were separated by 1% (w/v) agarose gel electrophoresis and stained with ethidium bromide. (1) 26.0 ng; (2) 2.6 ng; (3) 0.26 ng; (4) 26.0 pg; (5) 2.6 pg; (6) 0.26 pg; (7) 26.0 fg; (8) blank control (double-distilled water).

    Journal: Frontiers in Microbiology

    Article Title: Surveillance of Dihydropteroate Synthase Genes in Stenotrophomonas maltophilia by LAMP: Implications for Infection Control and Initial Therapy

    doi: 10.3389/fmicb.2016.01723

    Figure Lengend Snippet: Comparison of the sensitivities of LAMP and conventional PCR assays for detecting sul2 in S. maltophilia . (A) Turbidity was monitored every 6 s using a Loopamp Realtime Turbidimeter with detection at 650 nm. The reaction was performed at 65°C. (B) The results were visualized by adding HNB to the reaction mixture before the reaction. (C) PCR products were separated by 1% (w/v) agarose gel electrophoresis and stained with ethidium bromide. (1) 26.0 ng; (2) 2.6 ng; (3) 0.26 ng; (4) 26.0 pg; (5) 2.6 pg; (6) 0.26 pg; (7) 26.0 fg; (8) blank control (double-distilled water).

    Article Snippet: Real-time changes in turbidity were monitored by spectrophotometric analysis by recording the optical density (650 nm) every 6 s with a Loopamp Realtime Turbidimeter (LA-320c; Eiken Chemical, Co., Ltd).

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Staining

    Comparison of the sensitivities of LAMP and conventional PCR assays for detecting sul1 in Stenotrophomonas maltophilia . (A) Turbidity was monitored every 6 s using a Loopamp Realtime Turbidimeter with detection at 650 nm. The reaction was performed at 65°C. (B) The results were visualized by adding hydroxy naphthol blue (HNB) to the reaction mixture before the reaction. (C) PCR products were separated by 1% (w/v) agarose gel electrophoresis and stained with ethidium bromide. (1) 74.0 ng; (2) 7.4 ng; (3) 0.74 ng; (4) 74.0 pg; (5) 7.4 pg; (6) 0.74 pg; (7) 74.0 fg; (8) 7.4 fg; (9) blank control (double-distilled water).

    Journal: Frontiers in Microbiology

    Article Title: Surveillance of Dihydropteroate Synthase Genes in Stenotrophomonas maltophilia by LAMP: Implications for Infection Control and Initial Therapy

    doi: 10.3389/fmicb.2016.01723

    Figure Lengend Snippet: Comparison of the sensitivities of LAMP and conventional PCR assays for detecting sul1 in Stenotrophomonas maltophilia . (A) Turbidity was monitored every 6 s using a Loopamp Realtime Turbidimeter with detection at 650 nm. The reaction was performed at 65°C. (B) The results were visualized by adding hydroxy naphthol blue (HNB) to the reaction mixture before the reaction. (C) PCR products were separated by 1% (w/v) agarose gel electrophoresis and stained with ethidium bromide. (1) 74.0 ng; (2) 7.4 ng; (3) 0.74 ng; (4) 74.0 pg; (5) 7.4 pg; (6) 0.74 pg; (7) 74.0 fg; (8) 7.4 fg; (9) blank control (double-distilled water).

    Article Snippet: Real-time changes in turbidity were monitored by spectrophotometric analysis by recording the optical density (650 nm) every 6 s with a Loopamp Realtime Turbidimeter (LA-320c; Eiken Chemical, Co., Ltd).

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Staining

    Specificity of the LAMP reaction for detecting sul1 in S. maltophilia . (A) Turbidity was monitored every 6 s using a Loopamp Realtime Turbidimeter with detection at 650 nm. The reaction was performed at 65°C. (B) The results were visualized by adding HNB to the reaction mixture before the reaction. (1) X2 (positive control for sul1 from Peking Union Medical College Hospital); (2) P121 (positive control for sul1 from Chinese PLA General Hospital); (3) K106 (positive control for sul1 from Chinese PLA General Hospital); (4) S. maltophilia K279a; (5) S. maltophilia ATCC 13637; (6) P129 (negative control for sul from Chinese PLA General Hospital, confirmed by genome sequencing); (7) double-distilled water.

    Journal: Frontiers in Microbiology

    Article Title: Surveillance of Dihydropteroate Synthase Genes in Stenotrophomonas maltophilia by LAMP: Implications for Infection Control and Initial Therapy

    doi: 10.3389/fmicb.2016.01723

    Figure Lengend Snippet: Specificity of the LAMP reaction for detecting sul1 in S. maltophilia . (A) Turbidity was monitored every 6 s using a Loopamp Realtime Turbidimeter with detection at 650 nm. The reaction was performed at 65°C. (B) The results were visualized by adding HNB to the reaction mixture before the reaction. (1) X2 (positive control for sul1 from Peking Union Medical College Hospital); (2) P121 (positive control for sul1 from Chinese PLA General Hospital); (3) K106 (positive control for sul1 from Chinese PLA General Hospital); (4) S. maltophilia K279a; (5) S. maltophilia ATCC 13637; (6) P129 (negative control for sul from Chinese PLA General Hospital, confirmed by genome sequencing); (7) double-distilled water.

    Article Snippet: Real-time changes in turbidity were monitored by spectrophotometric analysis by recording the optical density (650 nm) every 6 s with a Loopamp Realtime Turbidimeter (LA-320c; Eiken Chemical, Co., Ltd).

    Techniques: Positive Control, Proximity Ligation Assay, Negative Control, Sequencing

    Specificity of the LAMP reaction for detecting sul2 in S. maltophilia. (A) Turbidity was monitored every 6 s using a Loopamp Realtime Turbidimeter with detection at 650 nm. The reaction was performed at 65°C. (B) The results were visualized by adding HNB to the reaction mixture before the reaction. (1) X133 (positive control for sul2 from Peking Union Medical College Hospital); (2) P57 (positive control for sul2 from Chinese PLA General Hospital); (3) K14 (positive control for sul2 from Chinese PLA General Hospital); (4) S. maltophilia K279a; (5) S. maltophilia ATCC 13637; (6) P129 (negative control for sul from Chinese PLA General Hospital, confirmed by genome sequencing); (7) double-distilled water.

    Journal: Frontiers in Microbiology

    Article Title: Surveillance of Dihydropteroate Synthase Genes in Stenotrophomonas maltophilia by LAMP: Implications for Infection Control and Initial Therapy

    doi: 10.3389/fmicb.2016.01723

    Figure Lengend Snippet: Specificity of the LAMP reaction for detecting sul2 in S. maltophilia. (A) Turbidity was monitored every 6 s using a Loopamp Realtime Turbidimeter with detection at 650 nm. The reaction was performed at 65°C. (B) The results were visualized by adding HNB to the reaction mixture before the reaction. (1) X133 (positive control for sul2 from Peking Union Medical College Hospital); (2) P57 (positive control for sul2 from Chinese PLA General Hospital); (3) K14 (positive control for sul2 from Chinese PLA General Hospital); (4) S. maltophilia K279a; (5) S. maltophilia ATCC 13637; (6) P129 (negative control for sul from Chinese PLA General Hospital, confirmed by genome sequencing); (7) double-distilled water.

    Article Snippet: Real-time changes in turbidity were monitored by spectrophotometric analysis by recording the optical density (650 nm) every 6 s with a Loopamp Realtime Turbidimeter (LA-320c; Eiken Chemical, Co., Ltd).

    Techniques: Positive Control, Proximity Ligation Assay, Negative Control, Sequencing