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Eiken Chemical loopamp real time turbidimeter
Relationship of turbidity with copy number of the 2009 H1N1 influenza A virus. Real‐time turbidity was detected with a <t>Loopamp</t> real‐time turbidimeter. The time required to reach the threshold turbidity level (0.1) (threshold time) were plotted against the copy numbers of the samples determined by real‐time RT‐PCR.
Loopamp Real Time Turbidimeter, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Mobile and accurate detection system for infection by the 2009 pandemic influenza A (H1N1) virus with a pocket‐warmer reverse‐transcriptase loop‐mediated isothermal amplification"

Article Title: Mobile and accurate detection system for infection by the 2009 pandemic influenza A (H1N1) virus with a pocket‐warmer reverse‐transcriptase loop‐mediated isothermal amplification

Journal: Journal of Medical Virology

doi: 10.1002/jmv.22031

Relationship of turbidity with copy number of the 2009 H1N1 influenza A virus. Real‐time turbidity was detected with a Loopamp real‐time turbidimeter. The time required to reach the threshold turbidity level (0.1) (threshold time) were plotted against the copy numbers of the samples determined by real‐time RT‐PCR.
Figure Legend Snippet: Relationship of turbidity with copy number of the 2009 H1N1 influenza A virus. Real‐time turbidity was detected with a Loopamp real‐time turbidimeter. The time required to reach the threshold turbidity level (0.1) (threshold time) were plotted against the copy numbers of the samples determined by real‐time RT‐PCR.

Techniques Used: Quantitative RT-PCR

2) Product Images from "Development of Loop-Mediated Isothermal Amplification Targeting 18S Ribosomal DNA for Rapid Detection of Azumiobodo hoyamushi (Kinetoplastea)"

Article Title: Development of Loop-Mediated Isothermal Amplification Targeting 18S Ribosomal DNA for Rapid Detection of Azumiobodo hoyamushi (Kinetoplastea)

Journal: The Korean Journal of Parasitology

doi: 10.3347/kjp.2014.52.3.305

Detection limit of Azumiobodo hoyamushi 18S rDNA LAMP assays (A). LAMP assays were performed using serial dilutions of A. hoyamushi genomic DNA (1 ng to 1 fg per reaction). Distilled water was used as a negative control. LAMP products were visualized by gel electrophoresis (B) and using Loopamp® fluorescent detection reagent (FD) (C). (B, C) Lane M, 100-bp DNA marker; lane 1, 1 ng; lane 2, 100 pg; lane 3, 10 pg; lane 4, 1 pg; lane 5, 100 fg; lane 6, 10 fg; lane 7, 1 fg of A. hoyamushi genomic DNA; lane 8, distilled water; and lane 9, LAMP product after Mbo I digestion. (D-E) A. hoyamushi at a density of 1×10 3 parasites/µl was serially diluted and tested (D) using the LAMP assay (D) and by PCR (E) using F3 and B3 primers. Lane M, 100-bp DNA marker; lane 1, 1,000; lane 2, 100; lane 3, 10; lane 4, 1; lane 5, 0.1; lane 6, 0.01 of parasites per reaction; lane 7, distilled water. A. hoyamushi genomic DNA was prepared using DNeasy tissue kits (Qiagen) from in vitro cultured A. hoyamushi species [ 9 ] which were kindly provided by Dr. Kyung Il Park (Kunsan National University, Gunsan, Korea).
Figure Legend Snippet: Detection limit of Azumiobodo hoyamushi 18S rDNA LAMP assays (A). LAMP assays were performed using serial dilutions of A. hoyamushi genomic DNA (1 ng to 1 fg per reaction). Distilled water was used as a negative control. LAMP products were visualized by gel electrophoresis (B) and using Loopamp® fluorescent detection reagent (FD) (C). (B, C) Lane M, 100-bp DNA marker; lane 1, 1 ng; lane 2, 100 pg; lane 3, 10 pg; lane 4, 1 pg; lane 5, 100 fg; lane 6, 10 fg; lane 7, 1 fg of A. hoyamushi genomic DNA; lane 8, distilled water; and lane 9, LAMP product after Mbo I digestion. (D-E) A. hoyamushi at a density of 1×10 3 parasites/µl was serially diluted and tested (D) using the LAMP assay (D) and by PCR (E) using F3 and B3 primers. Lane M, 100-bp DNA marker; lane 1, 1,000; lane 2, 100; lane 3, 10; lane 4, 1; lane 5, 0.1; lane 6, 0.01 of parasites per reaction; lane 7, distilled water. A. hoyamushi genomic DNA was prepared using DNeasy tissue kits (Qiagen) from in vitro cultured A. hoyamushi species [ 9 ] which were kindly provided by Dr. Kyung Il Park (Kunsan National University, Gunsan, Korea).

Techniques Used: Negative Control, Nucleic Acid Electrophoresis, Marker, Lamp Assay, Polymerase Chain Reaction, In Vitro, Cell Culture

3) Product Images from "Rapid Identification of Officinal Akebiae Caulis and Its Toxic Adulterant Aristolochiae Manshuriensis Caulis (Aristolochia manshuriensis) by Loop-Mediated Isothermal Amplification"

Article Title: Rapid Identification of Officinal Akebiae Caulis and Its Toxic Adulterant Aristolochiae Manshuriensis Caulis (Aristolochia manshuriensis) by Loop-Mediated Isothermal Amplification

Journal: Frontiers in Plant Science

doi: 10.3389/fpls.2016.00887

Differentiation of Akebiae Caulis from Aristolochiae Manshuriensis Caulis using LAMP. Gmt-1 to Gmt-3, Aristolochia manshuriensis ; Mt-1 to Mt-3, Akebia trifoliata ; Mt-4 to Mt-6, Ak. trifoliata var. australis ; Mt-7 to Mt-9, Ak. quinata ; Neg, negative control (double-distilled water). (A) Turbidity was monitored by a Loopamp real-time turbidimeter at 400 nm every 6 s, amplification was performed at 65°C for 60 min; (B) A visual color change detection method was compared. 1 μl of calcein (fluorescent detection reagent) was added to 25 μl of LAMP reaction mixture before the LAMP reaction.
Figure Legend Snippet: Differentiation of Akebiae Caulis from Aristolochiae Manshuriensis Caulis using LAMP. Gmt-1 to Gmt-3, Aristolochia manshuriensis ; Mt-1 to Mt-3, Akebia trifoliata ; Mt-4 to Mt-6, Ak. trifoliata var. australis ; Mt-7 to Mt-9, Ak. quinata ; Neg, negative control (double-distilled water). (A) Turbidity was monitored by a Loopamp real-time turbidimeter at 400 nm every 6 s, amplification was performed at 65°C for 60 min; (B) A visual color change detection method was compared. 1 μl of calcein (fluorescent detection reagent) was added to 25 μl of LAMP reaction mixture before the LAMP reaction.

Techniques Used: Negative Control, Amplification

Comparison of sensitivity between the LAMP reaction and conventional PCR for detection of Aristolochiae Manshuriensis Caulis. The pure genomic DNA extracted from Aristolochia manshuriensis was diluted in a serial 10-fold dilution. Both LAMP reaction (A) and (B) and conventional PCR (C) were carried out in duplicate for each dilution point. Tubes and lanes: 1, 42.2 ng/μl; 2, 4.22 ng/μl; 3, 422 pg/μl; 4, 42.2 pg/μl; 5, 4.22 pg/μl; 6, 422 fg/μl; 7, 42.2 fg/μl; 8, Neg, negative control (double-distilled water). (A) Turbidity was monitored by a Loopamp real-time turbidimeter at 400 nm every 6 s; (B) A visual color change detection method was compared. 1μl of calcein (fluorescent detection reagent) was added to 25 μl of LAMP reaction mixture before the LAMP reaction; (C) The PCR products were detected by 1% agarose gel electrophoresis.
Figure Legend Snippet: Comparison of sensitivity between the LAMP reaction and conventional PCR for detection of Aristolochiae Manshuriensis Caulis. The pure genomic DNA extracted from Aristolochia manshuriensis was diluted in a serial 10-fold dilution. Both LAMP reaction (A) and (B) and conventional PCR (C) were carried out in duplicate for each dilution point. Tubes and lanes: 1, 42.2 ng/μl; 2, 4.22 ng/μl; 3, 422 pg/μl; 4, 42.2 pg/μl; 5, 4.22 pg/μl; 6, 422 fg/μl; 7, 42.2 fg/μl; 8, Neg, negative control (double-distilled water). (A) Turbidity was monitored by a Loopamp real-time turbidimeter at 400 nm every 6 s; (B) A visual color change detection method was compared. 1μl of calcein (fluorescent detection reagent) was added to 25 μl of LAMP reaction mixture before the LAMP reaction; (C) The PCR products were detected by 1% agarose gel electrophoresis.

Techniques Used: Polymerase Chain Reaction, Negative Control, Agarose Gel Electrophoresis

4) Product Images from "Sensitive and Rapid Detection of the Plasmid-Encoded Colistin-Resistance Gene mcr-1 in Enterobacteriaceae Isolates by Loop-Mediated Isothermal Amplification"

Article Title: Sensitive and Rapid Detection of the Plasmid-Encoded Colistin-Resistance Gene mcr-1 in Enterobacteriaceae Isolates by Loop-Mediated Isothermal Amplification

Journal: Frontiers in Microbiology

doi: 10.3389/fmicb.2017.02356

LAMP assay primer screening. Three sets of primers amplified the target gene under the same conditions. Turbidity was monitored by a Loopamp real-time turbidimeter at 650 nm every 6 s.
Figure Legend Snippet: LAMP assay primer screening. Three sets of primers amplified the target gene under the same conditions. Turbidity was monitored by a Loopamp real-time turbidimeter at 650 nm every 6 s.

Techniques Used: Lamp Assay, Amplification

5) Product Images from "Rapid detection of the common avian leukosis virus subgroups by real-time loop-mediated isothermal amplification"

Article Title: Rapid detection of the common avian leukosis virus subgroups by real-time loop-mediated isothermal amplification

Journal: Virology Journal

doi: 10.1186/s12985-015-0430-1

Sensitivity of the LAMP detections. Turbidity was monitored by the Loopamp real-time turbidimeter at 400 nm. The detection limits for normal exogenous ALV was 2 × 10 1 copies/μl of positive samples
Figure Legend Snippet: Sensitivity of the LAMP detections. Turbidity was monitored by the Loopamp real-time turbidimeter at 400 nm. The detection limits for normal exogenous ALV was 2 × 10 1 copies/μl of positive samples

Techniques Used:

6) Product Images from "Loop-Mediated Isothermal Amplification Targeting Actin DNA of Trichomonas vaginalis"

Article Title: Loop-Mediated Isothermal Amplification Targeting Actin DNA of Trichomonas vaginalis

Journal: The Korean Journal of Parasitology

doi: 10.3347/kjp.2016.54.3.329

Functionality of T. vaginalis actin LAMP assays. (A) LAMP on 10-fold serial dilutions of T. vaginalis genomic DNA (10 ng to 1 pg per reaction) monitored by measuring absorbance. Distilled water was used as a negative control. (B) LAMP products were visualized by gel electrophoresis. Lane 1, 1 ng; lane 2, 100 pg; lane 3, 10 pg; lane 4, 1 pg of T. vaginalis genomic DNA; lane 5, LAMP product after Hind III digestion; lane 6, distilled water; lane M, 100-bp DNA marker. (C) LAMP products were visualized under UV light using the Loopamp fluorescent detection reagent. Lane 1, 1 ng; lane 2, 100 pg; lane 3, 10 pg; lane 4, 1 pg; lane 5, 100 fg; lane 6, 10 fg of T. vaginalis genomic DNA; lane 7, distilled water. (D-E) T. vaginalis at a density of 1×10 2 parasites/μl was serially diluted and tested using the LAMP assay (D) and PCR (E) with F3 and B3 primers (Table 1). Lane M, 100-bp DNA marker; lane 1, 100; lane 2, 10; lane 3, 1; lane 4, 0.1; lane 5, 0.01 parasite(s) per reaction; lane 6, positive control, 100 pg of plasmid DNA containing the LAMP targeting regions of actin gene; lane 7, distilled water. (F) Specificity of LAMP primers for detection of T. vaginalis assessed using template DNA from other microbial species. Lane 1, T. vaginalis ; lane 2, Candida albicans ; lane 3, Chlamydia trachomatis ; lane 4, Neisseria gonorrhoeae ; lane 5, Cryptosporidium parvum ; lane 6, Entamoeba histolytica ; lane 7, Giardia lamblia ; lane 8, Escherichia coli ; lane 9, human genomic DNA. LAMP products were visualized by a color change that was also observable by the naked eye under normal visible light.
Figure Legend Snippet: Functionality of T. vaginalis actin LAMP assays. (A) LAMP on 10-fold serial dilutions of T. vaginalis genomic DNA (10 ng to 1 pg per reaction) monitored by measuring absorbance. Distilled water was used as a negative control. (B) LAMP products were visualized by gel electrophoresis. Lane 1, 1 ng; lane 2, 100 pg; lane 3, 10 pg; lane 4, 1 pg of T. vaginalis genomic DNA; lane 5, LAMP product after Hind III digestion; lane 6, distilled water; lane M, 100-bp DNA marker. (C) LAMP products were visualized under UV light using the Loopamp fluorescent detection reagent. Lane 1, 1 ng; lane 2, 100 pg; lane 3, 10 pg; lane 4, 1 pg; lane 5, 100 fg; lane 6, 10 fg of T. vaginalis genomic DNA; lane 7, distilled water. (D-E) T. vaginalis at a density of 1×10 2 parasites/μl was serially diluted and tested using the LAMP assay (D) and PCR (E) with F3 and B3 primers (Table 1). Lane M, 100-bp DNA marker; lane 1, 100; lane 2, 10; lane 3, 1; lane 4, 0.1; lane 5, 0.01 parasite(s) per reaction; lane 6, positive control, 100 pg of plasmid DNA containing the LAMP targeting regions of actin gene; lane 7, distilled water. (F) Specificity of LAMP primers for detection of T. vaginalis assessed using template DNA from other microbial species. Lane 1, T. vaginalis ; lane 2, Candida albicans ; lane 3, Chlamydia trachomatis ; lane 4, Neisseria gonorrhoeae ; lane 5, Cryptosporidium parvum ; lane 6, Entamoeba histolytica ; lane 7, Giardia lamblia ; lane 8, Escherichia coli ; lane 9, human genomic DNA. LAMP products were visualized by a color change that was also observable by the naked eye under normal visible light.

Techniques Used: Negative Control, Nucleic Acid Electrophoresis, Marker, Lamp Assay, Polymerase Chain Reaction, Positive Control, Plasmid Preparation

7) Product Images from "Rapid and visual detection of 2019 novel coronavirus (SARS-CoV-2) by a reverse transcription loop-mediated isothermal amplification assay"

Article Title: Rapid and visual detection of 2019 novel coronavirus (SARS-CoV-2) by a reverse transcription loop-mediated isothermal amplification assay

Journal: Clinical Microbiology and Infection

doi: 10.1016/j.cmi.2020.04.001

Specificity of the reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for SARS-CoV-2 detection. A, B: Specificity of the RT-LAMP assay with primer set orf1ab-4 targeted the orf1ab gene for SARS-CoV-2 detection; C, D: Specificity of the RT-LAMP assay with primer set S-123 targeted the S gene for SARS-CoV-2 detection. The detection was monitored by turbidity using a Loopamp real-time turbidimeter (A1, B1), and was judged by the naked eye depending on a color change from orange to green (A2, B2). S1: HCoV-229E-1; S2: HCoV-229E-2; S3: HCoV-NL63-1; S4: HCoV-NL63-2; S5: HCoV-NL63-3; S6: HCoV-OC43-1; S7: HCoV-OC43-2; S8: HCoV-HKU1-1; S9: HCoV-HKU1-2; S10: H3N2-1; S11: H3N2-2; S12: H3N2-3; S13: H3N2-4; S14: H1N1-1; S15: H1N1-2; S16: influenza B-1; S17: influenza B-2; S18: influenza B-3; S19: PIV-1-1; S20: PIV-1-2; S21: PIV-2-1; S22: PIV-2-2; S23: PIV-3-1; S24: PIV-3-2; S25: PIV-4-1; S26: PIV-4-2; S27: ADV-1-1; S28: ADV-1-2; S29: ADV-2-1; S30: ADV-2-2; S31: ADV-3-1; S32: ADV-3-2; S33: ADV-4-1; S34: ADV-5-1; S35: ADV-5-2; S36: ADV-6-1; S37: ADV-7-1; S38: ADV-7-2; S39: RSV A-1; S40: RSV A-2; S41: RSV B-1; S42: RSV B-2; S43: HMPV-1; S44: HMPV-2; S45: BoV-1; S46: BoV-2; S47: Rh A-1; S48: RhA-2; S49: Rh B-1; S50: RhB-2; S51: Rh C-1; S52: MP-FH; S53: MP-M129; S54: Haemophilus influenzae ; S55: Staphylococcus aureus ; S56: Klebsiella pneumoniae ; S57: Streptococcus pneumoniae ; S58: Pseudomonas aeruginosa ; S59: SARS-CoV; S60: MERS-CoV; PC, positive control (pseudo-virus); NC, negative control (distilled water).
Figure Legend Snippet: Specificity of the reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for SARS-CoV-2 detection. A, B: Specificity of the RT-LAMP assay with primer set orf1ab-4 targeted the orf1ab gene for SARS-CoV-2 detection; C, D: Specificity of the RT-LAMP assay with primer set S-123 targeted the S gene for SARS-CoV-2 detection. The detection was monitored by turbidity using a Loopamp real-time turbidimeter (A1, B1), and was judged by the naked eye depending on a color change from orange to green (A2, B2). S1: HCoV-229E-1; S2: HCoV-229E-2; S3: HCoV-NL63-1; S4: HCoV-NL63-2; S5: HCoV-NL63-3; S6: HCoV-OC43-1; S7: HCoV-OC43-2; S8: HCoV-HKU1-1; S9: HCoV-HKU1-2; S10: H3N2-1; S11: H3N2-2; S12: H3N2-3; S13: H3N2-4; S14: H1N1-1; S15: H1N1-2; S16: influenza B-1; S17: influenza B-2; S18: influenza B-3; S19: PIV-1-1; S20: PIV-1-2; S21: PIV-2-1; S22: PIV-2-2; S23: PIV-3-1; S24: PIV-3-2; S25: PIV-4-1; S26: PIV-4-2; S27: ADV-1-1; S28: ADV-1-2; S29: ADV-2-1; S30: ADV-2-2; S31: ADV-3-1; S32: ADV-3-2; S33: ADV-4-1; S34: ADV-5-1; S35: ADV-5-2; S36: ADV-6-1; S37: ADV-7-1; S38: ADV-7-2; S39: RSV A-1; S40: RSV A-2; S41: RSV B-1; S42: RSV B-2; S43: HMPV-1; S44: HMPV-2; S45: BoV-1; S46: BoV-2; S47: Rh A-1; S48: RhA-2; S49: Rh B-1; S50: RhB-2; S51: Rh C-1; S52: MP-FH; S53: MP-M129; S54: Haemophilus influenzae ; S55: Staphylococcus aureus ; S56: Klebsiella pneumoniae ; S57: Streptococcus pneumoniae ; S58: Pseudomonas aeruginosa ; S59: SARS-CoV; S60: MERS-CoV; PC, positive control (pseudo-virus); NC, negative control (distilled water).

Techniques Used: Amplification, RT Lamp Assay, Positive Control, Negative Control

Sensitivity of the reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay and conventional PCR for SARS-CoV-2 detection. A, B: Sensitivity of the RT-LAMP assay using primer set orf1ab-4 to target the orf1ab gene for SARS-CoV-2 detection; D, E:Sensitivity of the RT-LAMP assay using primer set S-123 to target the S gene for SARS-CoV-2 detection; C, F:Sensitivity of the conventional PCR assay targeting the orf1ab and S genes for SARS-CoV-2 detection; The detection was monitored by turbidity using a Loopamp real-time turbidimeter, and was judged by the naked eye depending on a color change from orange to green. In B, 1-9: 10 8 , 10 7 , 10 6 , 10 5 , 10 4 , 10 3 , 10 2 , 10 1 , and 10 0 copies/μL. In E, 1-8: 10 7 , 10 6 , 10 5 , 10 4 , 10 3 , 10 2 , 10 1 , and 10 0 copies/μL. In C and F, 1-8: 10 8 , 10 7 , 10 6 , 10 5 , 10 4 , 10 3 , 10 2 , 10 1 , and 10 0 copies/μL. The reaction volume of 25 μL contained 2 μL RNA template, and the template concentration was 1ng/μL. In the sensitivity test, 60 min can be used as the cut-off for the visual detection.
Figure Legend Snippet: Sensitivity of the reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay and conventional PCR for SARS-CoV-2 detection. A, B: Sensitivity of the RT-LAMP assay using primer set orf1ab-4 to target the orf1ab gene for SARS-CoV-2 detection; D, E:Sensitivity of the RT-LAMP assay using primer set S-123 to target the S gene for SARS-CoV-2 detection; C, F:Sensitivity of the conventional PCR assay targeting the orf1ab and S genes for SARS-CoV-2 detection; The detection was monitored by turbidity using a Loopamp real-time turbidimeter, and was judged by the naked eye depending on a color change from orange to green. In B, 1-9: 10 8 , 10 7 , 10 6 , 10 5 , 10 4 , 10 3 , 10 2 , 10 1 , and 10 0 copies/μL. In E, 1-8: 10 7 , 10 6 , 10 5 , 10 4 , 10 3 , 10 2 , 10 1 , and 10 0 copies/μL. In C and F, 1-8: 10 8 , 10 7 , 10 6 , 10 5 , 10 4 , 10 3 , 10 2 , 10 1 , and 10 0 copies/μL. The reaction volume of 25 μL contained 2 μL RNA template, and the template concentration was 1ng/μL. In the sensitivity test, 60 min can be used as the cut-off for the visual detection.

Techniques Used: Amplification, RT Lamp Assay, Polymerase Chain Reaction, Concentration Assay

8) Product Images from "Loop-Mediated Isothermal Amplification Targeting 18S Ribosomal DNA for Rapid Detection of Acanthamoeba"

Article Title: Loop-Mediated Isothermal Amplification Targeting 18S Ribosomal DNA for Rapid Detection of Acanthamoeba

Journal: The Korean Journal of Parasitology

doi: 10.3347/kjp.2013.51.3.269

Sensitivities and specificities of Acanthamoeba LAMP assays. LAMP assays were performed using serial dilutions of (A) plasmid DNA containing Acanthamoeba castellanii 18S rDNA (10, 10 2 , 10 3 , or 10 4 copies per reaction) and (B) genomic DNA (100, 10, or 1 pg). Plasmid containing no insert was used as a control. LAMP products were visualized by (C) gel electrophoresis and using (D) the Loopamp® fluorescent detection reagent (FD). Lanes M1 and M2, 1-kb and 100-bp molecular weight markers, respectively; lane 1, Acanthamoeba astronyxis ; lane 2, Acanthamoeba triangularis ; lane 3, Acanthamoeba rhysodes ; lane 4, Acanthamoeba castellanii ; lane 5, Acanthamoeba lugdunensis ; lane 6, Acanthamoeba polyphaga ; lane 7, Acanthamoeba quina ; lane 8, Acanthamoeba griffini ; lane 9, Acanthamoeba hatchetti ; lane 10, Acanthamoeba culbertsoni ; lane 11, Acanthamoeba healyi ; lane 12, Aspergillus fumigatus ; lane 13, Fusarium solani ; lane 14, Candida albicans ; lane 15, Entamoeba histolytica ; lane 16, Giardia lamblia ; lane 17, Escherichia coli ; lane 18, distilled water; and lane 18, Nco I digestion of the LAMP product of 18S rDNA.
Figure Legend Snippet: Sensitivities and specificities of Acanthamoeba LAMP assays. LAMP assays were performed using serial dilutions of (A) plasmid DNA containing Acanthamoeba castellanii 18S rDNA (10, 10 2 , 10 3 , or 10 4 copies per reaction) and (B) genomic DNA (100, 10, or 1 pg). Plasmid containing no insert was used as a control. LAMP products were visualized by (C) gel electrophoresis and using (D) the Loopamp® fluorescent detection reagent (FD). Lanes M1 and M2, 1-kb and 100-bp molecular weight markers, respectively; lane 1, Acanthamoeba astronyxis ; lane 2, Acanthamoeba triangularis ; lane 3, Acanthamoeba rhysodes ; lane 4, Acanthamoeba castellanii ; lane 5, Acanthamoeba lugdunensis ; lane 6, Acanthamoeba polyphaga ; lane 7, Acanthamoeba quina ; lane 8, Acanthamoeba griffini ; lane 9, Acanthamoeba hatchetti ; lane 10, Acanthamoeba culbertsoni ; lane 11, Acanthamoeba healyi ; lane 12, Aspergillus fumigatus ; lane 13, Fusarium solani ; lane 14, Candida albicans ; lane 15, Entamoeba histolytica ; lane 16, Giardia lamblia ; lane 17, Escherichia coli ; lane 18, distilled water; and lane 18, Nco I digestion of the LAMP product of 18S rDNA.

Techniques Used: Plasmid Preparation, Nucleic Acid Electrophoresis, Molecular Weight

9) Product Images from "Prevalence and detection of Stenotrophomonas maltophilia carrying metallo-β-lactamase blaL1 in Beijing, China"

Article Title: Prevalence and detection of Stenotrophomonas maltophilia carrying metallo-β-lactamase blaL1 in Beijing, China

Journal: Frontiers in Microbiology

doi: 10.3389/fmicb.2014.00692

Comparison of the sensitivities for bla L1 gene detection by LAMP and conventional PCR methods. Pure genomic DNA extracted from S. maltophilia- K279a was diluted tenfold (379.0 ng/μl to 0.00379 pg/μl) and the DNA assayed by LAMP (A,B) and PCR (C) . (A) Turbidity was monitored using the Loopamp real-time turbidimeter and the OD recorded at 650 nm, at 6 s intervals. (B) Visual inspection of the color change, post-LAMP assay, and in the presence of calcein/Mn 2+ complex. (C) PCR products were analyzed by 2% agarose gel electrophoresis and stained with ethidium bromide. The DNA marker is D2000 DNA Marker (Tiangen Biotech Co., Ltd.) The size is about 179 bp.
Figure Legend Snippet: Comparison of the sensitivities for bla L1 gene detection by LAMP and conventional PCR methods. Pure genomic DNA extracted from S. maltophilia- K279a was diluted tenfold (379.0 ng/μl to 0.00379 pg/μl) and the DNA assayed by LAMP (A,B) and PCR (C) . (A) Turbidity was monitored using the Loopamp real-time turbidimeter and the OD recorded at 650 nm, at 6 s intervals. (B) Visual inspection of the color change, post-LAMP assay, and in the presence of calcein/Mn 2+ complex. (C) PCR products were analyzed by 2% agarose gel electrophoresis and stained with ethidium bromide. The DNA marker is D2000 DNA Marker (Tiangen Biotech Co., Ltd.) The size is about 179 bp.

Techniques Used: Polymerase Chain Reaction, Lamp Assay, Agarose Gel Electrophoresis, Staining, Marker

Specificity of the LAMP method for bla L1 gene detection. It has two parts, (A) is the graphic and (B) is the photography of microtubes. The reaction proceeded at 65°C for 65 min. Turbidity was monitored in the Loopamp real-time turbidimeter and the OD (λ650nm) recorded at 6 s intervals. L1, Brucella suis 3572; L2, Bacillus megatherium 4623; L3, Vibrio carchariae 5732; L4, Acinetobacter baumannii B260; L5, Corynebacterium diphtheriae CMCC38001; L6, Acinetobacter baumannii H18; L7, Mycobacterium tuberculosis 8362; L8, Shigella sonnei 2531; L9, Shigella flexneri 4536; L10, Salmonella enteritidis 50326-1; L11, Yersinia enterocolitica 1836; L12, Vibrio parahaemolyticus 5474; L13, Salmonella paratyphi 86423; L14, Neisseria meningitidis group B CMCC29022; L15, Enterotoxigenic E. coli 44824; L16, Beta hemolytic Streptococcus group A CMCC32213; L17, Yersinia pestis 2638; L18, Salmonella aberdeen 9264; L19, Vibrio cholera 3802; L20, Staphylococcus aureus 2740; L21, Bordetella pertussis ATCC 18530 ; L22, positive control ( S. maltophilia - K279a); L23, negative control (distilled water).
Figure Legend Snippet: Specificity of the LAMP method for bla L1 gene detection. It has two parts, (A) is the graphic and (B) is the photography of microtubes. The reaction proceeded at 65°C for 65 min. Turbidity was monitored in the Loopamp real-time turbidimeter and the OD (λ650nm) recorded at 6 s intervals. L1, Brucella suis 3572; L2, Bacillus megatherium 4623; L3, Vibrio carchariae 5732; L4, Acinetobacter baumannii B260; L5, Corynebacterium diphtheriae CMCC38001; L6, Acinetobacter baumannii H18; L7, Mycobacterium tuberculosis 8362; L8, Shigella sonnei 2531; L9, Shigella flexneri 4536; L10, Salmonella enteritidis 50326-1; L11, Yersinia enterocolitica 1836; L12, Vibrio parahaemolyticus 5474; L13, Salmonella paratyphi 86423; L14, Neisseria meningitidis group B CMCC29022; L15, Enterotoxigenic E. coli 44824; L16, Beta hemolytic Streptococcus group A CMCC32213; L17, Yersinia pestis 2638; L18, Salmonella aberdeen 9264; L19, Vibrio cholera 3802; L20, Staphylococcus aureus 2740; L21, Bordetella pertussis ATCC 18530 ; L22, positive control ( S. maltophilia - K279a); L23, negative control (distilled water).

Techniques Used: Positive Control, Negative Control

Loop-mediated isothermal amplification results for 15 S. maltophilia strains positive for bla L1 isolated from 15 clinical samples. (A) Turbidity was monitored using Loopamp, and the OD measured at 650 nm every 6 s. (B) Visual inspection of calcein/Mn 2+ complex associated color changes post-LAMP assay. 1, S. maltophilia -2; 2, S. maltophilia -17; 3, S. maltophilia -24; 4, S. maltophilia -25; 5, S. maltophilia -36; 6, S. maltophilia -41; 7, S. maltophilia -51; 8, S. maltophilia -58; 9, S. maltophilia -63; 10, S. maltophilia -65; 11, S. maltophilia -66; 12, S. maltophilia -67; 13, S. maltophilia -3859; 14, S. maltophilia -4621; 15, S. maltophilia -WJ2; 16, positive control ( S. maltophilia- K279a); 17, negative control (distilled water).
Figure Legend Snippet: Loop-mediated isothermal amplification results for 15 S. maltophilia strains positive for bla L1 isolated from 15 clinical samples. (A) Turbidity was monitored using Loopamp, and the OD measured at 650 nm every 6 s. (B) Visual inspection of calcein/Mn 2+ complex associated color changes post-LAMP assay. 1, S. maltophilia -2; 2, S. maltophilia -17; 3, S. maltophilia -24; 4, S. maltophilia -25; 5, S. maltophilia -36; 6, S. maltophilia -41; 7, S. maltophilia -51; 8, S. maltophilia -58; 9, S. maltophilia -63; 10, S. maltophilia -65; 11, S. maltophilia -66; 12, S. maltophilia -67; 13, S. maltophilia -3859; 14, S. maltophilia -4621; 15, S. maltophilia -WJ2; 16, positive control ( S. maltophilia- K279a); 17, negative control (distilled water).

Techniques Used: Amplification, Isolation, Lamp Assay, Positive Control, Negative Control

10) Product Images from "Sensitive and Rapid Detection of the New Delhi Metallo-Beta-Lactamase Gene by Loop-Mediated Isothermal Amplification"

Article Title: Sensitive and Rapid Detection of the New Delhi Metallo-Beta-Lactamase Gene by Loop-Mediated Isothermal Amplification

Journal: Journal of Clinical Microbiology

doi: 10.1128/JCM.06647-11

Different temperatures of the LAMP reaction for detection of NDM-1. Turbidity was monitored by a Loopamp real-time turbidimeter at 400 nm every 6 s.
Figure Legend Snippet: Different temperatures of the LAMP reaction for detection of NDM-1. Turbidity was monitored by a Loopamp real-time turbidimeter at 400 nm every 6 s.

Techniques Used:

Detection of the bla NDM-1 gene in simulated sputum samples (A), simulated urine samples (B), and simulated fecal samples (C) by a Loopamp real-time turbidimeter at 400 nm every 6 s. The concentration of pure genomic DNA extracted from A. baumannii XM in each simulated sputum sample is shown.
Figure Legend Snippet: Detection of the bla NDM-1 gene in simulated sputum samples (A), simulated urine samples (B), and simulated fecal samples (C) by a Loopamp real-time turbidimeter at 400 nm every 6 s. The concentration of pure genomic DNA extracted from A. baumannii XM in each simulated sputum sample is shown.

Techniques Used: Concentration Assay

Specificity of the LAMP reaction for detection of bla NDM-1 . Turbidity was monitored by a Loopamp real-time turbidimeter at 400 nm every 6 s. Amplification was performed at 65°C for 65 min. Lines: 1, negative control (double-distilled water); 2, A. baumannii XM; 3, A. baumannii H949; 4, A. baumannii F398; 5, A. baumannii B260; 6, A. baumannii H18; 7, S. sonnei 2531; 8, S. flexneri 4536; 9, S. enterica serotype Enteritidis 50326-1; 10, V. carchariae 5732; 11, S. enterica serotype Paratyphi 86423; 12, enteroinvasive E. coli 44825; 13, enterotoxigenic E. coli 44824; 14, enteropathogenic E. coli 2348; and 15, V. parahaemolyticus 5474.
Figure Legend Snippet: Specificity of the LAMP reaction for detection of bla NDM-1 . Turbidity was monitored by a Loopamp real-time turbidimeter at 400 nm every 6 s. Amplification was performed at 65°C for 65 min. Lines: 1, negative control (double-distilled water); 2, A. baumannii XM; 3, A. baumannii H949; 4, A. baumannii F398; 5, A. baumannii B260; 6, A. baumannii H18; 7, S. sonnei 2531; 8, S. flexneri 4536; 9, S. enterica serotype Enteritidis 50326-1; 10, V. carchariae 5732; 11, S. enterica serotype Paratyphi 86423; 12, enteroinvasive E. coli 44825; 13, enterotoxigenic E. coli 44824; 14, enteropathogenic E. coli 2348; and 15, V. parahaemolyticus 5474.

Techniques Used: Amplification, Negative Control

Comparison of sensitivity between the LAMP reaction and PCR for detection of the bla NDM-1 gene. The pure genomic DNA extracted from A. baumannii XM was diluted in a serial 10-fold dilution. Both LAMP reactions (A and B) and PCRs (C) were carried out in duplicate for each dilution point. Tubes and lanes: 1, 1,070 ng/μl; 2, 107.0 ng/μl; 3, 10.70 ng/μl; 4, 1.070 ng/μl; 5, 107.0 pg/μl; 6, 10.70 pg/μl; 7, 1.070 pg/μl; 8, 0.107 pg/μl. (A) Turbidity was monitored by a Loopamp real-time turbidimeter at 400 nm every 6 s; (B) 1 μl of fluorescent detection reagent was added to 25 μl of LAMP reaction mixture before the LAMP reaction; (C) the PCR products were analyzed by 2% agarose gel electrophoresis and stained with ethidium bromide.
Figure Legend Snippet: Comparison of sensitivity between the LAMP reaction and PCR for detection of the bla NDM-1 gene. The pure genomic DNA extracted from A. baumannii XM was diluted in a serial 10-fold dilution. Both LAMP reactions (A and B) and PCRs (C) were carried out in duplicate for each dilution point. Tubes and lanes: 1, 1,070 ng/μl; 2, 107.0 ng/μl; 3, 10.70 ng/μl; 4, 1.070 ng/μl; 5, 107.0 pg/μl; 6, 10.70 pg/μl; 7, 1.070 pg/μl; 8, 0.107 pg/μl. (A) Turbidity was monitored by a Loopamp real-time turbidimeter at 400 nm every 6 s; (B) 1 μl of fluorescent detection reagent was added to 25 μl of LAMP reaction mixture before the LAMP reaction; (C) the PCR products were analyzed by 2% agarose gel electrophoresis and stained with ethidium bromide.

Techniques Used: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Staining

11) Product Images from "Survey and Visual Detection of Zaire ebolavirus in Clinical Samples Targeting the Nucleoprotein Gene in Sierra Leone"

Article Title: Survey and Visual Detection of Zaire ebolavirus in Clinical Samples Targeting the Nucleoprotein Gene in Sierra Leone

Journal: Frontiers in Microbiology

doi: 10.3389/fmicb.2015.01332

Specificity of EBOV NP detection by RT-LAMP. (A) Turbidity was monitored and recorded every 6 s by a Loopamp real-time turbidimeter at 650 nm. (B) Visual detection using a calcein fluorescent detection reagent. Lane 1, positive control (artificial EBOV RNA); lane 2, negative control (double-distilled water); lane 3, Sudan EBOV (artificial Sudan EBOV RNA); lane 4, MARV (artificial MARV RNA); lane 5, SARS coronavirus; lane 6, H7N9; lane 7, H1N1; lane 8, H2N3; lanes 9–12, human parainfluenza viruses (PIV) 1, 2, 3, and 4; lanes 13–15, adenoviruses (ADV; serotypes 3, 5, and 55); lanes 16 and 17, respiratory syncytial virus infection, RSVA, RSVB; lane 18, MERS RNA; lane 19, human metapneumovirus, HMPV; lane 20, bocavirus, BoV; lanes 21–24, human coronavirus, HCoV-229E, HCoV-OC43, HCoV-NL63, and HCoV-HKU1; lane 25, Legionella pneumophila 9135; lane 26, Mycobacterium tuberculosis 005; and lane 27, Haemophilus influenza ATCC 49247.
Figure Legend Snippet: Specificity of EBOV NP detection by RT-LAMP. (A) Turbidity was monitored and recorded every 6 s by a Loopamp real-time turbidimeter at 650 nm. (B) Visual detection using a calcein fluorescent detection reagent. Lane 1, positive control (artificial EBOV RNA); lane 2, negative control (double-distilled water); lane 3, Sudan EBOV (artificial Sudan EBOV RNA); lane 4, MARV (artificial MARV RNA); lane 5, SARS coronavirus; lane 6, H7N9; lane 7, H1N1; lane 8, H2N3; lanes 9–12, human parainfluenza viruses (PIV) 1, 2, 3, and 4; lanes 13–15, adenoviruses (ADV; serotypes 3, 5, and 55); lanes 16 and 17, respiratory syncytial virus infection, RSVA, RSVB; lane 18, MERS RNA; lane 19, human metapneumovirus, HMPV; lane 20, bocavirus, BoV; lanes 21–24, human coronavirus, HCoV-229E, HCoV-OC43, HCoV-NL63, and HCoV-HKU1; lane 25, Legionella pneumophila 9135; lane 26, Mycobacterium tuberculosis 005; and lane 27, Haemophilus influenza ATCC 49247.

Techniques Used: Positive Control, Negative Control, Infection

The most appropriate primers and reaction temperatures for the reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay. Turbidity was monitored and recorded every 6 s for five sets of primers used to amplify the target gene with a Loopamp real-time turbidimeter at 650 nm. (A) A total of five sets of primers including EBL-1, EBL-2, EBL-7, EBL-11, and EBL-16 were designed to detect artificial EBOV RNA. (B) Reaction temperatures ranged from 53 to 67°C with 2°C intervals.
Figure Legend Snippet: The most appropriate primers and reaction temperatures for the reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay. Turbidity was monitored and recorded every 6 s for five sets of primers used to amplify the target gene with a Loopamp real-time turbidimeter at 650 nm. (A) A total of five sets of primers including EBL-1, EBL-2, EBL-7, EBL-11, and EBL-16 were designed to detect artificial EBOV RNA. (B) Reaction temperatures ranged from 53 to 67°C with 2°C intervals.

Techniques Used: Amplification, RT Lamp Assay

Comparison of RT-LAMP sensitivities in detecting EBOV NP . Artificial EBOV RNA was serially diluted 10-fold from 4.56 × 10 4 copies/μL to 4.56 × 10 -2 copies/μL. (A) Turbidity was monitored with a Loopamp Realtime Turbidimeter at 650 nm every 6 s. (B) The reaction was detected visually using a calcein fluorescent detection reagent. Artificial EBOV RNA concentrations were: tube 1, 4.56 × 10 4 copies/μL; tube 2, 4.56 × 10 3 copies/μL; tube 3, 4.56 × 10 2 copies/μL; tube 4, 4.56 × 10 1 copies/μL; tube 5, 4.56 copies/μL; tube 6, 4.56 × 10 -1 copies/μL; tube 7, 4.56 × 10 -2 copies/μL; tube 8, ddH 2 O.
Figure Legend Snippet: Comparison of RT-LAMP sensitivities in detecting EBOV NP . Artificial EBOV RNA was serially diluted 10-fold from 4.56 × 10 4 copies/μL to 4.56 × 10 -2 copies/μL. (A) Turbidity was monitored with a Loopamp Realtime Turbidimeter at 650 nm every 6 s. (B) The reaction was detected visually using a calcein fluorescent detection reagent. Artificial EBOV RNA concentrations were: tube 1, 4.56 × 10 4 copies/μL; tube 2, 4.56 × 10 3 copies/μL; tube 3, 4.56 × 10 2 copies/μL; tube 4, 4.56 × 10 1 copies/μL; tube 5, 4.56 copies/μL; tube 6, 4.56 × 10 -1 copies/μL; tube 7, 4.56 × 10 -2 copies/μL; tube 8, ddH 2 O.

Techniques Used:

Related Articles

Incubation:

Article Title: Mobile and accurate detection system for infection by the 2009 pandemic influenza A (H1N1) virus with a pocket‐warmer reverse‐transcriptase loop‐mediated isothermal amplification
Article Snippet: .. For the conventional RT‐LAMP method, 50 µl of RT‐LAMP mixture including 9 µl of RNA sample was incubated in a Loopamp Real‐Time Turbidimeter (LA‐320C; Eiken Chemical) for 60 min at 60°C and then for 5 min at 80°C to terminate the reaction. .. Real‐Time RT‐PCR for the 2009 H1N1 Influenza A Virus TaqMan real‐time RT‐PCR was performed to measure the copy numbers of the 2009 H1N1 influenza A virus with a Mx3005P real‐time PCR system (Stratagene, La Jolla, CA) and a QuantiTect Probe RT‐PCR kit (Qiagen) as described previously [Nakajima et al., ], using 1 µl of extracted RNA as the template in each reaction mixture.

Amplification:

Article Title: Sensitive and Rapid Detection of the Plasmid-Encoded Colistin-Resistance Gene mcr-1 in Enterobacteriaceae Isolates by Loop-Mediated Isothermal Amplification
Article Snippet: .. For monitoring of turbidity, real-time amplification by the LAMP assay was monitored using a Loopamp real-time turbidimeter (LA320-C; Eiken Chemical Co. Ltd., Tokyo, Japan). ..

Article Title: Rapid Identification of Officinal Akebiae Caulis and Its Toxic Adulterant Aristolochiae Manshuriensis Caulis (Aristolochia manshuriensis) by Loop-Mediated Isothermal Amplification
Article Snippet: .. First, we monitored real-time amplification of LAMP assays using a Loopamp real-time turbidimeter (LA-230; Eiken Chemical Co., Ltd., Tochigi, Japan) to monitor turbidity by recording the optical density at 400 nm every 6 s. Second, we employed visual inspection where we observed color changes in the LAMP reaction mixtures, caused by the addition of 1 μl of calcein (Fluorescence Detection Reagent; Loopamp, Eiken China Co., Ltd., Shanghai, China) before the LAMP reaction. .. Third, we monitored fluorescence of the EvaGreen dye in a Rotor-Gene Q.

Lamp Assay:

Article Title: Sensitive and Rapid Detection of the Plasmid-Encoded Colistin-Resistance Gene mcr-1 in Enterobacteriaceae Isolates by Loop-Mediated Isothermal Amplification
Article Snippet: .. For monitoring of turbidity, real-time amplification by the LAMP assay was monitored using a Loopamp real-time turbidimeter (LA320-C; Eiken Chemical Co. Ltd., Tokyo, Japan). ..

Fluorescence:

Article Title: Rapid Identification of Officinal Akebiae Caulis and Its Toxic Adulterant Aristolochiae Manshuriensis Caulis (Aristolochia manshuriensis) by Loop-Mediated Isothermal Amplification
Article Snippet: .. First, we monitored real-time amplification of LAMP assays using a Loopamp real-time turbidimeter (LA-230; Eiken Chemical Co., Ltd., Tochigi, Japan) to monitor turbidity by recording the optical density at 400 nm every 6 s. Second, we employed visual inspection where we observed color changes in the LAMP reaction mixtures, caused by the addition of 1 μl of calcein (Fluorescence Detection Reagent; Loopamp, Eiken China Co., Ltd., Shanghai, China) before the LAMP reaction. .. Third, we monitored fluorescence of the EvaGreen dye in a Rotor-Gene Q.

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    Eiken Chemical loopamp real time turbidimeter
    Relationship of turbidity with copy number of the 2009 H1N1 influenza A virus. Real‐time turbidity was detected with a <t>Loopamp</t> real‐time turbidimeter. The time required to reach the threshold turbidity level (0.1) (threshold time) were plotted against the copy numbers of the samples determined by real‐time RT‐PCR.
    Loopamp Real Time Turbidimeter, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/loopamp real time turbidimeter/product/Eiken Chemical
    Average 91 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    loopamp real time turbidimeter - by Bioz Stars, 2020-08
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    Eiken Chemical loopamp real time la 320c turbidimeter
    Sensitivity of the hMPV genotype-specific RT-LAMP assay. Serial diluted RNAs ranging from 10 −6 to 10 −13 were used to determine the detection limits of the hMPV genotype-specific RT-LAMP. The results of the genotype-specific RT-LAMP were assessed by the <t>Loopamp</t> ® real-time <t>LA-320C</t> turbidimeter.
    Loopamp Real Time La 320c Turbidimeter, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/loopamp real time la 320c turbidimeter/product/Eiken Chemical
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    loopamp real time la 320c turbidimeter - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

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    Relationship of turbidity with copy number of the 2009 H1N1 influenza A virus. Real‐time turbidity was detected with a Loopamp real‐time turbidimeter. The time required to reach the threshold turbidity level (0.1) (threshold time) were plotted against the copy numbers of the samples determined by real‐time RT‐PCR.

    Journal: Journal of Medical Virology

    Article Title: Mobile and accurate detection system for infection by the 2009 pandemic influenza A (H1N1) virus with a pocket‐warmer reverse‐transcriptase loop‐mediated isothermal amplification

    doi: 10.1002/jmv.22031

    Figure Lengend Snippet: Relationship of turbidity with copy number of the 2009 H1N1 influenza A virus. Real‐time turbidity was detected with a Loopamp real‐time turbidimeter. The time required to reach the threshold turbidity level (0.1) (threshold time) were plotted against the copy numbers of the samples determined by real‐time RT‐PCR.

    Article Snippet: For the conventional RT‐LAMP method, 50 µl of RT‐LAMP mixture including 9 µl of RNA sample was incubated in a Loopamp Real‐Time Turbidimeter (LA‐320C; Eiken Chemical) for 60 min at 60°C and then for 5 min at 80°C to terminate the reaction.

    Techniques: Quantitative RT-PCR

    Detection limit of Azumiobodo hoyamushi 18S rDNA LAMP assays (A). LAMP assays were performed using serial dilutions of A. hoyamushi genomic DNA (1 ng to 1 fg per reaction). Distilled water was used as a negative control. LAMP products were visualized by gel electrophoresis (B) and using Loopamp® fluorescent detection reagent (FD) (C). (B, C) Lane M, 100-bp DNA marker; lane 1, 1 ng; lane 2, 100 pg; lane 3, 10 pg; lane 4, 1 pg; lane 5, 100 fg; lane 6, 10 fg; lane 7, 1 fg of A. hoyamushi genomic DNA; lane 8, distilled water; and lane 9, LAMP product after Mbo I digestion. (D-E) A. hoyamushi at a density of 1×10 3 parasites/µl was serially diluted and tested (D) using the LAMP assay (D) and by PCR (E) using F3 and B3 primers. Lane M, 100-bp DNA marker; lane 1, 1,000; lane 2, 100; lane 3, 10; lane 4, 1; lane 5, 0.1; lane 6, 0.01 of parasites per reaction; lane 7, distilled water. A. hoyamushi genomic DNA was prepared using DNeasy tissue kits (Qiagen) from in vitro cultured A. hoyamushi species [ 9 ] which were kindly provided by Dr. Kyung Il Park (Kunsan National University, Gunsan, Korea).

    Journal: The Korean Journal of Parasitology

    Article Title: Development of Loop-Mediated Isothermal Amplification Targeting 18S Ribosomal DNA for Rapid Detection of Azumiobodo hoyamushi (Kinetoplastea)

    doi: 10.3347/kjp.2014.52.3.305

    Figure Lengend Snippet: Detection limit of Azumiobodo hoyamushi 18S rDNA LAMP assays (A). LAMP assays were performed using serial dilutions of A. hoyamushi genomic DNA (1 ng to 1 fg per reaction). Distilled water was used as a negative control. LAMP products were visualized by gel electrophoresis (B) and using Loopamp® fluorescent detection reagent (FD) (C). (B, C) Lane M, 100-bp DNA marker; lane 1, 1 ng; lane 2, 100 pg; lane 3, 10 pg; lane 4, 1 pg; lane 5, 100 fg; lane 6, 10 fg; lane 7, 1 fg of A. hoyamushi genomic DNA; lane 8, distilled water; and lane 9, LAMP product after Mbo I digestion. (D-E) A. hoyamushi at a density of 1×10 3 parasites/µl was serially diluted and tested (D) using the LAMP assay (D) and by PCR (E) using F3 and B3 primers. Lane M, 100-bp DNA marker; lane 1, 1,000; lane 2, 100; lane 3, 10; lane 4, 1; lane 5, 0.1; lane 6, 0.01 of parasites per reaction; lane 7, distilled water. A. hoyamushi genomic DNA was prepared using DNeasy tissue kits (Qiagen) from in vitro cultured A. hoyamushi species [ 9 ] which were kindly provided by Dr. Kyung Il Park (Kunsan National University, Gunsan, Korea).

    Article Snippet: A. hoyamushi LAMP was performed for 90 min at 64℃ in a 25 µl mixture containing 40 pmol each of FIP and BIP, 5 pmol each of F3 and B3, 20 pmol each of LF and LB, 1.4 mM of deoxynucleoside triphosphates, 0.8 M betaine, and 1 µl of Bst DNA polymerase (NEB, Beverly, Massachusetts, USA) in 2.5 µl of buffer [20 mM Tris-HCl pH 8.8, 10 mM KCl, 10 mM (NH4 )2 SO4 , 8 mM MgSO4 , and 0.1% Tween 20], and 1 µl of template DNA in a Loopamp real-time turbidimeter (Realoop-30; Eiken Chemical Co., Tokyo, Japan).

    Techniques: Negative Control, Nucleic Acid Electrophoresis, Marker, Lamp Assay, Polymerase Chain Reaction, In Vitro, Cell Culture

    Differentiation of Akebiae Caulis from Aristolochiae Manshuriensis Caulis using LAMP. Gmt-1 to Gmt-3, Aristolochia manshuriensis ; Mt-1 to Mt-3, Akebia trifoliata ; Mt-4 to Mt-6, Ak. trifoliata var. australis ; Mt-7 to Mt-9, Ak. quinata ; Neg, negative control (double-distilled water). (A) Turbidity was monitored by a Loopamp real-time turbidimeter at 400 nm every 6 s, amplification was performed at 65°C for 60 min; (B) A visual color change detection method was compared. 1 μl of calcein (fluorescent detection reagent) was added to 25 μl of LAMP reaction mixture before the LAMP reaction.

    Journal: Frontiers in Plant Science

    Article Title: Rapid Identification of Officinal Akebiae Caulis and Its Toxic Adulterant Aristolochiae Manshuriensis Caulis (Aristolochia manshuriensis) by Loop-Mediated Isothermal Amplification

    doi: 10.3389/fpls.2016.00887

    Figure Lengend Snippet: Differentiation of Akebiae Caulis from Aristolochiae Manshuriensis Caulis using LAMP. Gmt-1 to Gmt-3, Aristolochia manshuriensis ; Mt-1 to Mt-3, Akebia trifoliata ; Mt-4 to Mt-6, Ak. trifoliata var. australis ; Mt-7 to Mt-9, Ak. quinata ; Neg, negative control (double-distilled water). (A) Turbidity was monitored by a Loopamp real-time turbidimeter at 400 nm every 6 s, amplification was performed at 65°C for 60 min; (B) A visual color change detection method was compared. 1 μl of calcein (fluorescent detection reagent) was added to 25 μl of LAMP reaction mixture before the LAMP reaction.

    Article Snippet: First, we monitored real-time amplification of LAMP assays using a Loopamp real-time turbidimeter (LA-230; Eiken Chemical Co., Ltd., Tochigi, Japan) to monitor turbidity by recording the optical density at 400 nm every 6 s. Second, we employed visual inspection where we observed color changes in the LAMP reaction mixtures, caused by the addition of 1 μl of calcein (Fluorescence Detection Reagent; Loopamp, Eiken China Co., Ltd., Shanghai, China) before the LAMP reaction.

    Techniques: Negative Control, Amplification

    Comparison of sensitivity between the LAMP reaction and conventional PCR for detection of Aristolochiae Manshuriensis Caulis. The pure genomic DNA extracted from Aristolochia manshuriensis was diluted in a serial 10-fold dilution. Both LAMP reaction (A) and (B) and conventional PCR (C) were carried out in duplicate for each dilution point. Tubes and lanes: 1, 42.2 ng/μl; 2, 4.22 ng/μl; 3, 422 pg/μl; 4, 42.2 pg/μl; 5, 4.22 pg/μl; 6, 422 fg/μl; 7, 42.2 fg/μl; 8, Neg, negative control (double-distilled water). (A) Turbidity was monitored by a Loopamp real-time turbidimeter at 400 nm every 6 s; (B) A visual color change detection method was compared. 1μl of calcein (fluorescent detection reagent) was added to 25 μl of LAMP reaction mixture before the LAMP reaction; (C) The PCR products were detected by 1% agarose gel electrophoresis.

    Journal: Frontiers in Plant Science

    Article Title: Rapid Identification of Officinal Akebiae Caulis and Its Toxic Adulterant Aristolochiae Manshuriensis Caulis (Aristolochia manshuriensis) by Loop-Mediated Isothermal Amplification

    doi: 10.3389/fpls.2016.00887

    Figure Lengend Snippet: Comparison of sensitivity between the LAMP reaction and conventional PCR for detection of Aristolochiae Manshuriensis Caulis. The pure genomic DNA extracted from Aristolochia manshuriensis was diluted in a serial 10-fold dilution. Both LAMP reaction (A) and (B) and conventional PCR (C) were carried out in duplicate for each dilution point. Tubes and lanes: 1, 42.2 ng/μl; 2, 4.22 ng/μl; 3, 422 pg/μl; 4, 42.2 pg/μl; 5, 4.22 pg/μl; 6, 422 fg/μl; 7, 42.2 fg/μl; 8, Neg, negative control (double-distilled water). (A) Turbidity was monitored by a Loopamp real-time turbidimeter at 400 nm every 6 s; (B) A visual color change detection method was compared. 1μl of calcein (fluorescent detection reagent) was added to 25 μl of LAMP reaction mixture before the LAMP reaction; (C) The PCR products were detected by 1% agarose gel electrophoresis.

    Article Snippet: First, we monitored real-time amplification of LAMP assays using a Loopamp real-time turbidimeter (LA-230; Eiken Chemical Co., Ltd., Tochigi, Japan) to monitor turbidity by recording the optical density at 400 nm every 6 s. Second, we employed visual inspection where we observed color changes in the LAMP reaction mixtures, caused by the addition of 1 μl of calcein (Fluorescence Detection Reagent; Loopamp, Eiken China Co., Ltd., Shanghai, China) before the LAMP reaction.

    Techniques: Polymerase Chain Reaction, Negative Control, Agarose Gel Electrophoresis

    Sensitivity of the hMPV genotype-specific RT-LAMP assay. Serial diluted RNAs ranging from 10 −6 to 10 −13 were used to determine the detection limits of the hMPV genotype-specific RT-LAMP. The results of the genotype-specific RT-LAMP were assessed by the Loopamp ® real-time LA-320C turbidimeter.

    Journal: Journal of Virological Methods

    Article Title: Identification of human metapneumovirus genotypes A and B from clinical specimens by reverse transcription loop-mediated isothermal amplification

    doi: 10.1016/j.jviromet.2013.10.037

    Figure Lengend Snippet: Sensitivity of the hMPV genotype-specific RT-LAMP assay. Serial diluted RNAs ranging from 10 −6 to 10 −13 were used to determine the detection limits of the hMPV genotype-specific RT-LAMP. The results of the genotype-specific RT-LAMP were assessed by the Loopamp ® real-time LA-320C turbidimeter.

    Article Snippet: A Loopamp® real-time LA-320C turbidimeter (Eiken Chemical, Tokyo, Japan) was used to monitor the accumulation of magnesium pyrophosphate spectrophotometrically at 650 nm.

    Techniques: RT Lamp Assay