Structured Review

Eiken Chemical loop mediated isothermal amplification lamp
Loop Mediated Isothermal Amplification Lamp, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 88/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/loop mediated isothermal amplification lamp/product/Eiken Chemical
Average 88 stars, based on 11 article reviews
Price from $9.99 to $1999.99
loop mediated isothermal amplification lamp - by Bioz Stars, 2020-12
88/100 stars

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Sequencing:

Article Title: Evaluation of point of care tests for the diagnosis of cutaneous leishmaniasis in Suriname
Article Snippet: .. The second test is a NAAT based on loop mediated isothermal amplification (LAMP) of a conserved region in the 18S rRNA gene of Leishmania species and a specific sequence in the kinetoplast DNA of L. donovani [ ], which is being marketed as Loopamp™ Leishmania Detection kit (Eiken Chemical Co., Japan). .. It is noted that the Loopamp™ is technically spoken not a true PoC test, as DNA extraction is required, but it ease of performance and the limited infrastructure needed, makes the implementation of this molecular diagnostic almost near patient feasible.

Article Title: Evaluation of point of care tests for the diagnosis of cutaneous leishmaniasis in Suriname
Article Snippet: .. The second test is a NAAT based on loop mediated isothermal amplification (LAMP) of a conserved region in the 18S rRNA gene of Leishmania species and a specific sequence in the kinetoplast DNA of L. donovani [ ], which is being marketed as Loopamp™ Leishmania Detection kit (Eiken Chemical Co., Japan). .. It is noted that the Loopamp™ is technically spoken not a true PoC test, as DNA extraction is required, but it ease of performance and the limited infrastructure needed, makes the implementation of this molecular diagnostic almost near patient feasible.

Amplification:

Article Title: Detecting amplicons of loop‐mediated isothermal amplification. Detecting amplicons of loop‐mediated isothermal amplification
Article Snippet: .. 1 INTRODUCTION The loop‐mediated isothermal amplification (LAMP) method was developed by Notomi et al. and was patented by Eiken Chemical Co., Ltd (patent no. JP2000283862, WO2002024902A1). .. In LAMP assays, DNA is amplified by four specific primers (F3, FIP, BIP, and B3) at 60–65°C within 1 hr, although the speed of amplification can be accelerated by adding two loop primers (LF and LB).

Article Title: Feasibility and Performance of Loop-Mediated Isothermal Amplification Assay in the Diagnosis of Pulmonary Tuberculosis in Decentralized Settings in Eastern China
Article Snippet: .. Recently, loop-mediated isothermal amplification (LAMP) has been developed by Eiken Chemical Company Ltd. (Tokyo, Japan) for detection of MTBC from clinical specimens [ ]. ..

Article Title: Evaluation of point of care tests for the diagnosis of cutaneous leishmaniasis in Suriname
Article Snippet: .. The second test is a NAAT based on loop mediated isothermal amplification (LAMP) of a conserved region in the 18S rRNA gene of Leishmania species and a specific sequence in the kinetoplast DNA of L. donovani [ ], which is being marketed as Loopamp™ Leishmania Detection kit (Eiken Chemical Co., Japan). .. It is noted that the Loopamp™ is technically spoken not a true PoC test, as DNA extraction is required, but it ease of performance and the limited infrastructure needed, makes the implementation of this molecular diagnostic almost near patient feasible.

Article Title: "Piggy-Backing" on Diagnostic Platforms Brings Hope to Neglected Diseases: The Case of Sleeping Sickness
Article Snippet: .. The second technology, loop-mediated isothermal amplification (LAMP) of DNA, is a simple molecular method developed by Eiken Chemical in Japan. .. The LAMP test is a novel strategy for DNA amplification that relies on the auto-cycling strand displacement synthesis of DNA by Bst DNA polymerase under isothermal conditions (60–65°C) .

Article Title: Evaluation of point of care tests for the diagnosis of cutaneous leishmaniasis in Suriname
Article Snippet: .. The second test is a NAAT based on loop mediated isothermal amplification (LAMP) of a conserved region in the 18S rRNA gene of Leishmania species and a specific sequence in the kinetoplast DNA of L. donovani [ ], which is being marketed as Loopamp™ Leishmania Detection kit (Eiken Chemical Co., Japan). .. It is noted that the Loopamp™ is technically spoken not a true PoC test, as DNA extraction is required, but it ease of performance and the limited infrastructure needed, makes the implementation of this molecular diagnostic almost near patient feasible.

Article Title: Foodborne Outbreak of Group G Streptococcal Pharyngitis in a School Dormitory in Osaka, Japan
Article Snippet: .. DNA from Legionella and B. pertussis was extracted using a QIAamp DNA minikit (Qiagen, Hilden, Germany), and loop-mediated isothermal amplification (LAMP) was conducted using a Loopamp Legionella detection kit C and Loopamp Bordetella pertussis detection kit D (Eiken Chemical Co., Ltd., Tokyo, Japan). .. Viral genes of respiratory infection viruses were detected in some specimens but were not common pathogens in patients; bacteria of the genus Legionella and of B. pertussis and the measles virus were not detected in patients.

Article Title: Recombinant nucleocapsid protein-based IgG enzyme-linked immunosorbent assay for the serological diagnosis of SARS.
Article Snippet: .. SARS-CoV RNA amplification by a loop-mediated isothermal amplification (LAMP) method for detection of SARS-CoV The SARS-CoV RNA genome was amplified using Loopamp SARS CoV-detection kit (Eiken Chemical, Ohtawara, Japan) as reported previously with some modifications . .. RNA was isolated from the serum samples using QIAamp viral RNA mini kit (Qiagen, Germany).

Article Title: Rapid molecular assays for detection of tuberculosis
Article Snippet: .. Loop-mediated isothermal amplification (LAMP) (Eiken Chemical Co. Ltd., Tokyo, Japan) assay is an isothermal molecular method developed by Notomi et al. [ ]. ..

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  • 89
    Eiken Chemical lamp assay loop mediated isothermal amplification assays
    Comparative sensitivities of both <t>LAMP</t> assay (A,B) and traditional PCR (C) . Lane M: DL2000 Marker; Tubes 1–9 and lanes 1–9: 10-fold serial dilution pure genomic <t>DNA</t> extracted from B. pertussis (130 ng/μl to 0.0013 pg/μl); Tube 10 and lane 10: ddH 2 O as negative control.
    Lamp Assay Loop Mediated Isothermal Amplification Assays, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 89/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lamp assay loop mediated isothermal amplification assays/product/Eiken Chemical
    Average 89 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    lamp assay loop mediated isothermal amplification assays - by Bioz Stars, 2020-12
    89/100 stars
      Buy from Supplier

    88
    Eiken Chemical transcription loop mediated isothermal amplification reactions
    The most appropriate primers and reaction temperatures for the reverse <t>transcription</t> <t>loop-mediated</t> <t>isothermal</t> <t>amplification</t> (RT-LAMP) assay. Turbidity was monitored and recorded every 6 s for five sets of primers used to amplify the target gene with a Loopamp real-time turbidimeter at 650 nm. (A) A total of five sets of primers including EBL-1, EBL-2, EBL-7, EBL-11, and EBL-16 were designed to detect artificial EBOV RNA. (B) Reaction temperatures ranged from 53 to 67°C with 2°C intervals.
    Transcription Loop Mediated Isothermal Amplification Reactions, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transcription loop mediated isothermal amplification reactions/product/Eiken Chemical
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    transcription loop mediated isothermal amplification reactions - by Bioz Stars, 2020-12
    88/100 stars
      Buy from Supplier

    90
    Eiken Chemical loop mediated isothermal amplification lamp kits
    Cord blood CD25 + FoxP3 − CD127 + CD4 T cell frequency is higher in placental Plasmodium <t>LAMP</t> infection. Frequencies of cord blood CD4 + CD25 + FoxP3 − CD127 + cells are higher in infants with positive placental blood LAMP test indicating presence of Plasmodium <t>DNA</t> in placental blood (Student’s t test; n = 166)
    Loop Mediated Isothermal Amplification Lamp Kits, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/loop mediated isothermal amplification lamp kits/product/Eiken Chemical
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    loop mediated isothermal amplification lamp kits - by Bioz Stars, 2020-12
    90/100 stars
      Buy from Supplier

    91
    Eiken Chemical naat protocol loop mediated isothermal amplification lamp
    Temporal analysis specifically for the <t>LAMP</t> <t>NAAT</t> protocol that required 60–65 °C reaction temperature. Experimental characterization ( a ) indicated time constants below the required 15 min as well as successful regulation within the set temperature range for both solutions. The thermal transient simulation indicated a more pronounced difference: the setting time was significantly lower for the thermostat-regulated setup than the self-regulating setup ( b ).
    Naat Protocol Loop Mediated Isothermal Amplification Lamp, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/naat protocol loop mediated isothermal amplification lamp/product/Eiken Chemical
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    naat protocol loop mediated isothermal amplification lamp - by Bioz Stars, 2020-12
    91/100 stars
      Buy from Supplier

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    Comparative sensitivities of both LAMP assay (A,B) and traditional PCR (C) . Lane M: DL2000 Marker; Tubes 1–9 and lanes 1–9: 10-fold serial dilution pure genomic DNA extracted from B. pertussis (130 ng/μl to 0.0013 pg/μl); Tube 10 and lane 10: ddH 2 O as negative control.

    Journal: Frontiers in Public Health

    Article Title: Survey and Rapid Detection of Bordetella pertussis in Clinical Samples Targeting the BP485 in China

    doi: 10.3389/fpubh.2015.00039

    Figure Lengend Snippet: Comparative sensitivities of both LAMP assay (A,B) and traditional PCR (C) . Lane M: DL2000 Marker; Tubes 1–9 and lanes 1–9: 10-fold serial dilution pure genomic DNA extracted from B. pertussis (130 ng/μl to 0.0013 pg/μl); Tube 10 and lane 10: ddH 2 O as negative control.

    Article Snippet: LAMP assay Loop-mediated isothermal amplification assays were carried out using the Loopamp DNA Amplification kit (Eiken Chemical Co., Ltd., Tochigi, Japan).

    Techniques: Lamp Assay, Polymerase Chain Reaction, Marker, Serial Dilution, Negative Control

    The most appropriate primers and reaction temperatures for the reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay. Turbidity was monitored and recorded every 6 s for five sets of primers used to amplify the target gene with a Loopamp real-time turbidimeter at 650 nm. (A) A total of five sets of primers including EBL-1, EBL-2, EBL-7, EBL-11, and EBL-16 were designed to detect artificial EBOV RNA. (B) Reaction temperatures ranged from 53 to 67°C with 2°C intervals.

    Journal: Frontiers in Microbiology

    Article Title: Survey and Visual Detection of Zaire ebolavirus in Clinical Samples Targeting the Nucleoprotein Gene in Sierra Leone

    doi: 10.3389/fmicb.2015.01332

    Figure Lengend Snippet: The most appropriate primers and reaction temperatures for the reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay. Turbidity was monitored and recorded every 6 s for five sets of primers used to amplify the target gene with a Loopamp real-time turbidimeter at 650 nm. (A) A total of five sets of primers including EBL-1, EBL-2, EBL-7, EBL-11, and EBL-16 were designed to detect artificial EBOV RNA. (B) Reaction temperatures ranged from 53 to 67°C with 2°C intervals.

    Article Snippet: RT-LAMP Assays Reverse transcription loop-mediated isothermal amplification reactions were performed using a Loopamp RNA amplification kit (Eiken Chemical Co., Ltd., Tokyo, Japan) in a volume of 25 μL according to the manufacturer’s protocol.

    Techniques: Amplification, RT Lamp Assay

    Cord blood CD25 + FoxP3 − CD127 + CD4 T cell frequency is higher in placental Plasmodium LAMP infection. Frequencies of cord blood CD4 + CD25 + FoxP3 − CD127 + cells are higher in infants with positive placental blood LAMP test indicating presence of Plasmodium DNA in placental blood (Student’s t test; n = 166)

    Journal: Malaria Journal

    Article Title: Timing of in utero malaria exposure influences fetal CD4 T cell regulatory versus effector differentiation

    doi: 10.1186/s12936-016-1545-6

    Figure Lengend Snippet: Cord blood CD25 + FoxP3 − CD127 + CD4 T cell frequency is higher in placental Plasmodium LAMP infection. Frequencies of cord blood CD4 + CD25 + FoxP3 − CD127 + cells are higher in infants with positive placental blood LAMP test indicating presence of Plasmodium DNA in placental blood (Student’s t test; n = 166)

    Article Snippet: Clinical outcomes Mothers were evaluated throughout pregnancy for Plasmodium parasitaemia beginning at enrolment (12–20 weeks of gestational age), and additionally with routine monthly surveillance testing peripheral blood via loop-mediated isothermal amplification (LAMP) kits (Eiken Chemical) which detect Plasmodium DNA [ , ].

    Techniques: Infection

    Temporal analysis specifically for the LAMP NAAT protocol that required 60–65 °C reaction temperature. Experimental characterization ( a ) indicated time constants below the required 15 min as well as successful regulation within the set temperature range for both solutions. The thermal transient simulation indicated a more pronounced difference: the setting time was significantly lower for the thermostat-regulated setup than the self-regulating setup ( b ).

    Journal: Micromachines

    Article Title: Development of Temperature Control Solutions for Non-Instrumented Nucleic Acid Amplification Tests (NINAAT)

    doi: 10.3390/mi8060180

    Figure Lengend Snippet: Temporal analysis specifically for the LAMP NAAT protocol that required 60–65 °C reaction temperature. Experimental characterization ( a ) indicated time constants below the required 15 min as well as successful regulation within the set temperature range for both solutions. The thermal transient simulation indicated a more pronounced difference: the setting time was significantly lower for the thermostat-regulated setup than the self-regulating setup ( b ).

    Article Snippet: Spatiotemporal Performance Analysis for Specific NAAT Protocol Loop-mediated isothermal amplification (LAMP) is a highly sensitive and specific DNA amplification method developed by Eiken Chemical Co. [ ], which requires incubation for 15–60 min at 60–65 °C temperature.

    Techniques: