loop mediated isothermal amplification lamp reaction bacteriophage λ dna  (Promega)

 
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    Name:
    Lambda DNA
    Description:
    Generates molecular weight size markers used in nucleic acid gel analysis
    Catalog Number:
    d1501
    Price:
    None
    Category:
    Instrumentation Labware Biochemicals Labware Nucleic Acids
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    Structured Review

    Promega loop mediated isothermal amplification lamp reaction bacteriophage λ dna
    Characterization and evaluation of the iLAMP system. Evaluation of <t>DNA</t> capture by the chitosan-modified filter paper in the iLAMP device using <t>λ-DNA</t> samples. ( A ) The average DNA capture efficiencies were above 96% when input was between 5–20 ng (mean ± SD, n = 3). ( B ) The average capture efficiencies were above 96% when 10,000, 1000, or even 100 copies of λ-DNA were diluted in 1 mL MES (mean ± SD, n = 3). ( C ) Validation of on-chip amplifications of 0, 10 2 , 10 3 , 10 4 , and 10 6 copies of templates. The experiments were repeated three times and only one set of results were shown here. ( D ) The fitted curve between the log of the template copy number and the threshold time (Tt) in the fluorescence graphs (mean ± SD, R 2 = 0.994, n = 3).
    Generates molecular weight size markers used in nucleic acid gel analysis
    https://www.bioz.com/result/loop mediated isothermal amplification lamp reaction bacteriophage λ dna/product/Promega
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    loop mediated isothermal amplification lamp reaction bacteriophage λ dna - by Bioz Stars, 2020-12
    99/100 stars

    Related Products / Commonly Used Together

    dna extraction

    Images

    1) Product Images from "A Fully Integrated In Vitro Diagnostic Microsystem for Pathogen Detection Developed Using a “3D Extensible” Microfluidic Design Paradigm"

    Article Title: A Fully Integrated In Vitro Diagnostic Microsystem for Pathogen Detection Developed Using a “3D Extensible” Microfluidic Design Paradigm

    Journal: Micromachines

    doi: 10.3390/mi10120873

    Characterization and evaluation of the iLAMP system. Evaluation of DNA capture by the chitosan-modified filter paper in the iLAMP device using λ-DNA samples. ( A ) The average DNA capture efficiencies were above 96% when input was between 5–20 ng (mean ± SD, n = 3). ( B ) The average capture efficiencies were above 96% when 10,000, 1000, or even 100 copies of λ-DNA were diluted in 1 mL MES (mean ± SD, n = 3). ( C ) Validation of on-chip amplifications of 0, 10 2 , 10 3 , 10 4 , and 10 6 copies of templates. The experiments were repeated three times and only one set of results were shown here. ( D ) The fitted curve between the log of the template copy number and the threshold time (Tt) in the fluorescence graphs (mean ± SD, R 2 = 0.994, n = 3).
    Figure Legend Snippet: Characterization and evaluation of the iLAMP system. Evaluation of DNA capture by the chitosan-modified filter paper in the iLAMP device using λ-DNA samples. ( A ) The average DNA capture efficiencies were above 96% when input was between 5–20 ng (mean ± SD, n = 3). ( B ) The average capture efficiencies were above 96% when 10,000, 1000, or even 100 copies of λ-DNA were diluted in 1 mL MES (mean ± SD, n = 3). ( C ) Validation of on-chip amplifications of 0, 10 2 , 10 3 , 10 4 , and 10 6 copies of templates. The experiments were repeated three times and only one set of results were shown here. ( D ) The fitted curve between the log of the template copy number and the threshold time (Tt) in the fluorescence graphs (mean ± SD, R 2 = 0.994, n = 3).

    Techniques Used: Modification, Chromatin Immunoprecipitation, Fluorescence

    Design of the iLAMP system. ( A ) The schematic diagram of the iLAMP microdevice. v1–v9 represent the valve structures from the basic elements. c1 is the chamber on the chip. Volumes of each valve compartment and chamber are listed above corresponding structures. ( B ) The iLAMP device is comprised of a block, a piece of DS tape, and a chip covered with adhesive PCR foil. Reagents are prestored in valve compartments. ( C ) Photographs of the iLAMP device and the reaction chamber. A piece of chitosan-modified filter paper is embedded in the chamber for DNA capture and “in situ” LAMP. ( D ) Photograph of the control and detection instrument of the iLAMP device.
    Figure Legend Snippet: Design of the iLAMP system. ( A ) The schematic diagram of the iLAMP microdevice. v1–v9 represent the valve structures from the basic elements. c1 is the chamber on the chip. Volumes of each valve compartment and chamber are listed above corresponding structures. ( B ) The iLAMP device is comprised of a block, a piece of DS tape, and a chip covered with adhesive PCR foil. Reagents are prestored in valve compartments. ( C ) Photographs of the iLAMP device and the reaction chamber. A piece of chitosan-modified filter paper is embedded in the chamber for DNA capture and “in situ” LAMP. ( D ) Photograph of the control and detection instrument of the iLAMP device.

    Techniques Used: Chromatin Immunoprecipitation, Blocking Assay, Polymerase Chain Reaction, Modification

    Operation of the iLAMP microsystem. The total analytical time was about 82 min, including 22 min for DNA extraction and 60 min for LAMP.
    Figure Legend Snippet: Operation of the iLAMP microsystem. The total analytical time was about 82 min, including 22 min for DNA extraction and 60 min for LAMP.

    Techniques Used: DNA Extraction

    Related Articles

    DNA Extraction:

    Article Title: A Fully Integrated In Vitro Diagnostic Microsystem for Pathogen Detection Developed Using a “3D Extensible” Microfluidic Design Paradigm
    Article Snippet: .. DNA Extraction and Loop-Mediated Isothermal Amplification (LAMP) Reaction Bacteriophage λ-DNA (Promega, Madison, WI, USA) was employed to examine the DNA capture efficiency of the iLAMP microdevice. .. After on-chip capture of λ-DNA, the chitosan-modified filter paper was taken out and placed into tubes for real-time PCR on a Bio-Rad iQ5 system (Bio-Rad, Hercules, CA, USA).

    Amplification:

    Article Title: A Fully Integrated In Vitro Diagnostic Microsystem for Pathogen Detection Developed Using a “3D Extensible” Microfluidic Design Paradigm
    Article Snippet: .. DNA Extraction and Loop-Mediated Isothermal Amplification (LAMP) Reaction Bacteriophage λ-DNA (Promega, Madison, WI, USA) was employed to examine the DNA capture efficiency of the iLAMP microdevice. .. After on-chip capture of λ-DNA, the chitosan-modified filter paper was taken out and placed into tubes for real-time PCR on a Bio-Rad iQ5 system (Bio-Rad, Hercules, CA, USA).

    In Vitro:

    Article Title: Direct Fluorescent Imaging of Translocation and Unwinding by Individual DNA Helicases
    Article Snippet: .. The ligated recombinant λ DNA product is packaged into a λ capsid in vitro using the Packagene® Lambda DNA Packaging System (Promega, Madison, WI). ..

    Positive Control:

    Article Title: A DNase from a Fungal Phytopathogen Is a Virulence Factor Likely Deployed as Counter Defense against Host-Secreted Extracellular DNA
    Article Snippet: .. Reactions were run on 1% agarose gels at 100 V for 15 min. As a positive control for λ DNA degradation, 1 unit of RQ1 DNase (catalog no. M610A; Promega) was used. .. To test DNase activity of recombinant Nuc1 and Nuc2 proteins (purification described below), reaction mixtures contained 2.5 µg of λ DNA and 0.84 to 2.6 µg of the purified Nuc1 or 0.28 to 0.87 µg of Nuc2 MBP-fusion proteins.

    Lambda DNA Preparation:

    Article Title: Comprehensive DNA methylation analysis of the Aedes aegypti genome
    Article Snippet: .. As a spike-in control, unmethylated bacteriophage lambda DNA (Promega) and genomic DNA extracted from human blood (Blood & Cell Culture DNA Kit, Qiagen) were used. ..

    Article Title: Direct Fluorescent Imaging of Translocation and Unwinding by Individual DNA Helicases
    Article Snippet: .. The ligated recombinant λ DNA product is packaged into a λ capsid in vitro using the Packagene® Lambda DNA Packaging System (Promega, Madison, WI). ..

    Cell Culture:

    Article Title: Comprehensive DNA methylation analysis of the Aedes aegypti genome
    Article Snippet: .. As a spike-in control, unmethylated bacteriophage lambda DNA (Promega) and genomic DNA extracted from human blood (Blood & Cell Culture DNA Kit, Qiagen) were used. ..

    Significance Assay:

    Article Title: Bisulfite Conversion of DNA: Performance Comparison of Different Kits and Methylation Quantitation of Epigenetic Biomarkers that Have the Potential to Be Used in Non-Invasive Prenatal Testing
    Article Snippet: .. The λ-DNA control 1 was able to fit linear lines with R2 = 0.99 and 0.97 for the Promega and the Diagenode kit, respectively, indicating that both kits performed very well [P value for both samples < 0.05]. ..

    Labeling:

    Article Title: Binding Kinetics of Bisintercalator Triostin A with Optical Tweezers Force Mechanics
    Article Snippet: .. All experiments were conducted in our homebuilt fluid chamber, which is described in detail elsewhere ( ). λ -DNA (Promega, Madison, WI) was biochemically labeled with multiple biotin molecules at both ends. .. Streptavidin-coated microbeads (Spherotech, Lake Forest, IL; diameter 3.28 μ m), the biotin-labeled DNA, and Triostin A were dissolved in phosphate-buffered saline (136 mM NaCl, 2.7 mM KCl, 8.1 mM Na2 PO4 , 1.5 mM KH2 PO4 ) with pH 7.4 containing 1 μ M EDTA.

    Staining:

    Article Title: High Process Yield Rates of Thermoplastic Nanofluidic Devices using a Hybrid Thermal Assembly Technique
    Article Snippet: .. All images were analyzed using Fiji software. λ-DNA (Promega Corporation) and T4 DNA (Wako Chemicals) were stained with the bis-intercalating dye, YOYO-1 (Molecular Probes, Eugene, OR) at a base-pair/dye ratio of 5:1 in a buffer solution of 1× TBE (89 mM Tris, 89 mM Borate, 1 mM EDTA) with the addition of 4% v/v β-mercaptoethanol used as a radical scavenger to minimize photo-induced damage (photobleaching and/or photonicking). ..

    Article Title: Surface Charge, Electroosmotic Flow and DNA Extension in Chemically Modified Thermoplastic Nanoslits and Nanochannels
    Article Snippet: .. To study the electrokinetic parameters and extension length of λ-DNA, 100 × 100 nm nanochannels were used. λ-DNA (Promega Corporation) were stained with the bis-intercalating dye, YOYO-1 (Molecular Probes, Eugene, OR) at a base-pair/dye ratio of 5:1 in an electrolyte solution of 1X TBE (89 mM Tris, 89 mM Borate, 1 mM EDTA) with the addition of 4% v/v β-mercaptoethanol as a radical scavenger to minimize photo-induced damage (photobleaching and/or photonicking). ..

    Recombinant:

    Article Title: Direct Fluorescent Imaging of Translocation and Unwinding by Individual DNA Helicases
    Article Snippet: .. The ligated recombinant λ DNA product is packaged into a λ capsid in vitro using the Packagene® Lambda DNA Packaging System (Promega, Madison, WI). ..

    Software:

    Article Title: High Process Yield Rates of Thermoplastic Nanofluidic Devices using a Hybrid Thermal Assembly Technique
    Article Snippet: .. All images were analyzed using Fiji software. λ-DNA (Promega Corporation) and T4 DNA (Wako Chemicals) were stained with the bis-intercalating dye, YOYO-1 (Molecular Probes, Eugene, OR) at a base-pair/dye ratio of 5:1 in a buffer solution of 1× TBE (89 mM Tris, 89 mM Borate, 1 mM EDTA) with the addition of 4% v/v β-mercaptoethanol used as a radical scavenger to minimize photo-induced damage (photobleaching and/or photonicking). ..

    Similar Products

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  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Promega loop mediated isothermal amplification lamp reaction bacteriophage λ dna
    Characterization and evaluation of the iLAMP system. Evaluation of <t>DNA</t> capture by the chitosan-modified filter paper in the iLAMP device using <t>λ-DNA</t> samples. ( A ) The average DNA capture efficiencies were above 96% when input was between 5–20 ng (mean ± SD, n = 3). ( B ) The average capture efficiencies were above 96% when 10,000, 1000, or even 100 copies of λ-DNA were diluted in 1 mL MES (mean ± SD, n = 3). ( C ) Validation of on-chip amplifications of 0, 10 2 , 10 3 , 10 4 , and 10 6 copies of templates. The experiments were repeated three times and only one set of results were shown here. ( D ) The fitted curve between the log of the template copy number and the threshold time (Tt) in the fluorescence graphs (mean ± SD, R 2 = 0.994, n = 3).
    Loop Mediated Isothermal Amplification Lamp Reaction Bacteriophage λ Dna, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/loop mediated isothermal amplification lamp reaction bacteriophage λ dna/product/Promega
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    loop mediated isothermal amplification lamp reaction bacteriophage λ dna - by Bioz Stars, 2020-12
    99/100 stars
      Buy from Supplier

    Image Search Results


    Characterization and evaluation of the iLAMP system. Evaluation of DNA capture by the chitosan-modified filter paper in the iLAMP device using λ-DNA samples. ( A ) The average DNA capture efficiencies were above 96% when input was between 5–20 ng (mean ± SD, n = 3). ( B ) The average capture efficiencies were above 96% when 10,000, 1000, or even 100 copies of λ-DNA were diluted in 1 mL MES (mean ± SD, n = 3). ( C ) Validation of on-chip amplifications of 0, 10 2 , 10 3 , 10 4 , and 10 6 copies of templates. The experiments were repeated three times and only one set of results were shown here. ( D ) The fitted curve between the log of the template copy number and the threshold time (Tt) in the fluorescence graphs (mean ± SD, R 2 = 0.994, n = 3).

    Journal: Micromachines

    Article Title: A Fully Integrated In Vitro Diagnostic Microsystem for Pathogen Detection Developed Using a “3D Extensible” Microfluidic Design Paradigm

    doi: 10.3390/mi10120873

    Figure Lengend Snippet: Characterization and evaluation of the iLAMP system. Evaluation of DNA capture by the chitosan-modified filter paper in the iLAMP device using λ-DNA samples. ( A ) The average DNA capture efficiencies were above 96% when input was between 5–20 ng (mean ± SD, n = 3). ( B ) The average capture efficiencies were above 96% when 10,000, 1000, or even 100 copies of λ-DNA were diluted in 1 mL MES (mean ± SD, n = 3). ( C ) Validation of on-chip amplifications of 0, 10 2 , 10 3 , 10 4 , and 10 6 copies of templates. The experiments were repeated three times and only one set of results were shown here. ( D ) The fitted curve between the log of the template copy number and the threshold time (Tt) in the fluorescence graphs (mean ± SD, R 2 = 0.994, n = 3).

    Article Snippet: DNA Extraction and Loop-Mediated Isothermal Amplification (LAMP) Reaction Bacteriophage λ-DNA (Promega, Madison, WI, USA) was employed to examine the DNA capture efficiency of the iLAMP microdevice.

    Techniques: Modification, Chromatin Immunoprecipitation, Fluorescence

    Design of the iLAMP system. ( A ) The schematic diagram of the iLAMP microdevice. v1–v9 represent the valve structures from the basic elements. c1 is the chamber on the chip. Volumes of each valve compartment and chamber are listed above corresponding structures. ( B ) The iLAMP device is comprised of a block, a piece of DS tape, and a chip covered with adhesive PCR foil. Reagents are prestored in valve compartments. ( C ) Photographs of the iLAMP device and the reaction chamber. A piece of chitosan-modified filter paper is embedded in the chamber for DNA capture and “in situ” LAMP. ( D ) Photograph of the control and detection instrument of the iLAMP device.

    Journal: Micromachines

    Article Title: A Fully Integrated In Vitro Diagnostic Microsystem for Pathogen Detection Developed Using a “3D Extensible” Microfluidic Design Paradigm

    doi: 10.3390/mi10120873

    Figure Lengend Snippet: Design of the iLAMP system. ( A ) The schematic diagram of the iLAMP microdevice. v1–v9 represent the valve structures from the basic elements. c1 is the chamber on the chip. Volumes of each valve compartment and chamber are listed above corresponding structures. ( B ) The iLAMP device is comprised of a block, a piece of DS tape, and a chip covered with adhesive PCR foil. Reagents are prestored in valve compartments. ( C ) Photographs of the iLAMP device and the reaction chamber. A piece of chitosan-modified filter paper is embedded in the chamber for DNA capture and “in situ” LAMP. ( D ) Photograph of the control and detection instrument of the iLAMP device.

    Article Snippet: DNA Extraction and Loop-Mediated Isothermal Amplification (LAMP) Reaction Bacteriophage λ-DNA (Promega, Madison, WI, USA) was employed to examine the DNA capture efficiency of the iLAMP microdevice.

    Techniques: Chromatin Immunoprecipitation, Blocking Assay, Polymerase Chain Reaction, Modification

    Operation of the iLAMP microsystem. The total analytical time was about 82 min, including 22 min for DNA extraction and 60 min for LAMP.

    Journal: Micromachines

    Article Title: A Fully Integrated In Vitro Diagnostic Microsystem for Pathogen Detection Developed Using a “3D Extensible” Microfluidic Design Paradigm

    doi: 10.3390/mi10120873

    Figure Lengend Snippet: Operation of the iLAMP microsystem. The total analytical time was about 82 min, including 22 min for DNA extraction and 60 min for LAMP.

    Article Snippet: DNA Extraction and Loop-Mediated Isothermal Amplification (LAMP) Reaction Bacteriophage λ-DNA (Promega, Madison, WI, USA) was employed to examine the DNA capture efficiency of the iLAMP microdevice.

    Techniques: DNA Extraction