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Eiken Chemical loop mediated isothermal amplification assay
Loop Mediated Isothermal Amplification Assay, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/loop mediated isothermal amplification assay/product/Eiken Chemical
Average 93 stars, based on 3 article reviews
Price from $9.99 to $1999.99
loop mediated isothermal amplification assay - by Bioz Stars, 2020-12
93/100 stars

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Article Title: Prevalence of Vibrio parahaemolyticus in seafood and water environment in the Mekong Delta, Vietnam
Article Snippet: .. tdh gene: Loop-mediated isothermal amplification assay was applied to detect tdh gene using a Loopamp DNA amplification kit (Eiken Chemical Co., Ltd., Tokyo, Japan) as described by Yamazaki et al . .. The incubation was carried out in a Loop realtime tubidimeter (Realoop-30, Eiken Chemical) at 65°C for 60 min, following by 80°C for 2 min. A reaction was considered positive as the turbidity reached 0.1 within 60 min. trh gene: PCR assay for trh gene examination was performed using the protocol described previously [ ].

Article Title: Prevalence of Vibrio parahaemolyticus in seafood and water environment in the Mekong Delta, Vietnam
Article Snippet: .. Pathogenic gene detection tdh gene: Loop-mediated isothermal amplification assay was applied to detect tdh gene using a Loopamp DNA amplification kit (Eiken Chemical Co., Ltd., Tokyo, Japan) as described by Yamazaki et al . .. The incubation was carried out in a Loop realtime tubidimeter (Realoop-30, Eiken Chemical) at 65°C for 60 min, following by 80°C for 2 min. A reaction was considered positive as the turbidity reached 0.1 within 60 min. trh gene: PCR assay for trh gene examination was performed using the protocol described previously [ ].

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  • 89
    Eiken Chemical lamp assay loop mediated isothermal amplification assays
    Comparative sensitivities of both <t>LAMP</t> assay (A,B) and traditional PCR (C) . Lane M: DL2000 Marker; Tubes 1–9 and lanes 1–9: 10-fold serial dilution pure genomic <t>DNA</t> extracted from B. pertussis (130 ng/μl to 0.0013 pg/μl); Tube 10 and lane 10: ddH 2 O as negative control.
    Lamp Assay Loop Mediated Isothermal Amplification Assays, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 89/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lamp assay loop mediated isothermal amplification assays/product/Eiken Chemical
    Average 89 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    lamp assay loop mediated isothermal amplification assays - by Bioz Stars, 2020-12
    89/100 stars
      Buy from Supplier

    88
    Eiken Chemical transcription loop mediated isothermal amplification reactions
    The most appropriate primers and reaction temperatures for the reverse <t>transcription</t> <t>loop-mediated</t> <t>isothermal</t> <t>amplification</t> (RT-LAMP) assay. Turbidity was monitored and recorded every 6 s for five sets of primers used to amplify the target gene with a Loopamp real-time turbidimeter at 650 nm. (A) A total of five sets of primers including EBL-1, EBL-2, EBL-7, EBL-11, and EBL-16 were designed to detect artificial EBOV RNA. (B) Reaction temperatures ranged from 53 to 67°C with 2°C intervals.
    Transcription Loop Mediated Isothermal Amplification Reactions, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transcription loop mediated isothermal amplification reactions/product/Eiken Chemical
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    transcription loop mediated isothermal amplification reactions - by Bioz Stars, 2020-12
    88/100 stars
      Buy from Supplier

    90
    Eiken Chemical loop mediated isothermal amplification lamp kits
    Cord blood CD25 + FoxP3 − CD127 + CD4 T cell frequency is higher in placental Plasmodium <t>LAMP</t> infection. Frequencies of cord blood CD4 + CD25 + FoxP3 − CD127 + cells are higher in infants with positive placental blood LAMP test indicating presence of Plasmodium <t>DNA</t> in placental blood (Student’s t test; n = 166)
    Loop Mediated Isothermal Amplification Lamp Kits, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/loop mediated isothermal amplification lamp kits/product/Eiken Chemical
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    loop mediated isothermal amplification lamp kits - by Bioz Stars, 2020-12
    90/100 stars
      Buy from Supplier

    91
    Eiken Chemical naat protocol loop mediated isothermal amplification lamp
    Temporal analysis specifically for the <t>LAMP</t> <t>NAAT</t> protocol that required 60–65 °C reaction temperature. Experimental characterization ( a ) indicated time constants below the required 15 min as well as successful regulation within the set temperature range for both solutions. The thermal transient simulation indicated a more pronounced difference: the setting time was significantly lower for the thermostat-regulated setup than the self-regulating setup ( b ).
    Naat Protocol Loop Mediated Isothermal Amplification Lamp, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/naat protocol loop mediated isothermal amplification lamp/product/Eiken Chemical
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    naat protocol loop mediated isothermal amplification lamp - by Bioz Stars, 2020-12
    91/100 stars
      Buy from Supplier

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    Comparative sensitivities of both LAMP assay (A,B) and traditional PCR (C) . Lane M: DL2000 Marker; Tubes 1–9 and lanes 1–9: 10-fold serial dilution pure genomic DNA extracted from B. pertussis (130 ng/μl to 0.0013 pg/μl); Tube 10 and lane 10: ddH 2 O as negative control.

    Journal: Frontiers in Public Health

    Article Title: Survey and Rapid Detection of Bordetella pertussis in Clinical Samples Targeting the BP485 in China

    doi: 10.3389/fpubh.2015.00039

    Figure Lengend Snippet: Comparative sensitivities of both LAMP assay (A,B) and traditional PCR (C) . Lane M: DL2000 Marker; Tubes 1–9 and lanes 1–9: 10-fold serial dilution pure genomic DNA extracted from B. pertussis (130 ng/μl to 0.0013 pg/μl); Tube 10 and lane 10: ddH 2 O as negative control.

    Article Snippet: LAMP assay Loop-mediated isothermal amplification assays were carried out using the Loopamp DNA Amplification kit (Eiken Chemical Co., Ltd., Tochigi, Japan).

    Techniques: Lamp Assay, Polymerase Chain Reaction, Marker, Serial Dilution, Negative Control

    The most appropriate primers and reaction temperatures for the reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay. Turbidity was monitored and recorded every 6 s for five sets of primers used to amplify the target gene with a Loopamp real-time turbidimeter at 650 nm. (A) A total of five sets of primers including EBL-1, EBL-2, EBL-7, EBL-11, and EBL-16 were designed to detect artificial EBOV RNA. (B) Reaction temperatures ranged from 53 to 67°C with 2°C intervals.

    Journal: Frontiers in Microbiology

    Article Title: Survey and Visual Detection of Zaire ebolavirus in Clinical Samples Targeting the Nucleoprotein Gene in Sierra Leone

    doi: 10.3389/fmicb.2015.01332

    Figure Lengend Snippet: The most appropriate primers and reaction temperatures for the reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay. Turbidity was monitored and recorded every 6 s for five sets of primers used to amplify the target gene with a Loopamp real-time turbidimeter at 650 nm. (A) A total of five sets of primers including EBL-1, EBL-2, EBL-7, EBL-11, and EBL-16 were designed to detect artificial EBOV RNA. (B) Reaction temperatures ranged from 53 to 67°C with 2°C intervals.

    Article Snippet: RT-LAMP Assays Reverse transcription loop-mediated isothermal amplification reactions were performed using a Loopamp RNA amplification kit (Eiken Chemical Co., Ltd., Tokyo, Japan) in a volume of 25 μL according to the manufacturer’s protocol.

    Techniques: Amplification, RT Lamp Assay

    Cord blood CD25 + FoxP3 − CD127 + CD4 T cell frequency is higher in placental Plasmodium LAMP infection. Frequencies of cord blood CD4 + CD25 + FoxP3 − CD127 + cells are higher in infants with positive placental blood LAMP test indicating presence of Plasmodium DNA in placental blood (Student’s t test; n = 166)

    Journal: Malaria Journal

    Article Title: Timing of in utero malaria exposure influences fetal CD4 T cell regulatory versus effector differentiation

    doi: 10.1186/s12936-016-1545-6

    Figure Lengend Snippet: Cord blood CD25 + FoxP3 − CD127 + CD4 T cell frequency is higher in placental Plasmodium LAMP infection. Frequencies of cord blood CD4 + CD25 + FoxP3 − CD127 + cells are higher in infants with positive placental blood LAMP test indicating presence of Plasmodium DNA in placental blood (Student’s t test; n = 166)

    Article Snippet: Clinical outcomes Mothers were evaluated throughout pregnancy for Plasmodium parasitaemia beginning at enrolment (12–20 weeks of gestational age), and additionally with routine monthly surveillance testing peripheral blood via loop-mediated isothermal amplification (LAMP) kits (Eiken Chemical) which detect Plasmodium DNA [ , ].

    Techniques: Infection

    Temporal analysis specifically for the LAMP NAAT protocol that required 60–65 °C reaction temperature. Experimental characterization ( a ) indicated time constants below the required 15 min as well as successful regulation within the set temperature range for both solutions. The thermal transient simulation indicated a more pronounced difference: the setting time was significantly lower for the thermostat-regulated setup than the self-regulating setup ( b ).

    Journal: Micromachines

    Article Title: Development of Temperature Control Solutions for Non-Instrumented Nucleic Acid Amplification Tests (NINAAT)

    doi: 10.3390/mi8060180

    Figure Lengend Snippet: Temporal analysis specifically for the LAMP NAAT protocol that required 60–65 °C reaction temperature. Experimental characterization ( a ) indicated time constants below the required 15 min as well as successful regulation within the set temperature range for both solutions. The thermal transient simulation indicated a more pronounced difference: the setting time was significantly lower for the thermostat-regulated setup than the self-regulating setup ( b ).

    Article Snippet: Spatiotemporal Performance Analysis for Specific NAAT Protocol Loop-mediated isothermal amplification (LAMP) is a highly sensitive and specific DNA amplification method developed by Eiken Chemical Co. [ ], which requires incubation for 15–60 min at 60–65 °C temperature.

    Techniques: