long template genomic pcr  (Millipore)


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    Structured Review

    Millipore long template genomic pcr
    Long Template Genomic Pcr, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/long template genomic pcr/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    long template genomic pcr - by Bioz Stars, 2020-05
    94/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Haploinsufficiency for NR3C1, the gene encoding the glucocorticoid receptor, in blastic plasmacytoid dendritic cell neoplasms
    Article Snippet: .. Long-distance PCR (LD-PCR) cloning of the t(3;5)(q21;q31) genomic breakpoint was performed using the Expand Long Template PCR system (Sigma) and LD-PCR primers, TA cloning of PCR products, and sequencing (supplemental Table 6). .. For RNA analysis, total RNA was prepared by TRIzol reagent, according to the manufacturer’s instructions.

    Amplification:

    Article Title: Clock-dependent chromatin topology modulates circadian transcription and behavior
    Article Snippet: .. Six-hundred nanograms of 4C template was used for PCR amplification using Sigma-Aldrich long-template PCR system with bait-specific inverse primers conjugated to Illumina sequencing adaptors (primer sequences are in ) in a final volume of 50 µL in the following PCR program: 2 min at 94°C followed by 30 cycles of 15 sec at 94°C, 1 min at 55°C, and 3 min at 68°C and a final extension of 7 min at 68°C. .. PCR were performed in parallel reactions with 6 × 100 ng of template for each sample.

    Article Title: 53BP1 Supports Immunoglobulin Class Switch Recombination Independently of Its DNA Double-Strand Break End Protection Function
    Article Snippet: .. Genomic DNA was extracted from the sorted population, and Sμ-Sα junctions were amplified by Expand long template PCR system (Sigma Aldrich). ..

    Article Title: Developing a tetO/TetR system in Neurospora crassa.
    Article Snippet: .. The development of a tetO/TetR system in the fungus Neurospora crassa is described. .. The development of a tetO/TetR system in the fungus Neurospora crassa is described.

    Article Title: NanoSatellite: accurate characterization of expanded tandem repeat length and sequence through whole genome long-read sequencing on PromethION
    Article Snippet: .. PCR optimizations for all TRs were performed with the Expand Long Template PCR System (Sigma-Aldrich, St. Louis, USA) and LongAmp Taq (NEB), with and without the addition of betain, at annealing temperatures ranging from 45 to 65 °C and with sufficiently long elongation times to support efficient amplification of long DNA sequences. .. To confirm specificity, the PCR amplicons were Sanger sequenced using BigDye reagents (Thermo Fisher Scientific) on a 3730 DNA analyzer (Thermo Fisher Scientific).

    TA Cloning:

    Article Title: Haploinsufficiency for NR3C1, the gene encoding the glucocorticoid receptor, in blastic plasmacytoid dendritic cell neoplasms
    Article Snippet: .. Long-distance PCR (LD-PCR) cloning of the t(3;5)(q21;q31) genomic breakpoint was performed using the Expand Long Template PCR system (Sigma) and LD-PCR primers, TA cloning of PCR products, and sequencing (supplemental Table 6). .. For RNA analysis, total RNA was prepared by TRIzol reagent, according to the manufacturer’s instructions.

    Size-exclusion Chromatography:

    Article Title: Clock-dependent chromatin topology modulates circadian transcription and behavior
    Article Snippet: .. Six-hundred nanograms of 4C template was used for PCR amplification using Sigma-Aldrich long-template PCR system with bait-specific inverse primers conjugated to Illumina sequencing adaptors (primer sequences are in ) in a final volume of 50 µL in the following PCR program: 2 min at 94°C followed by 30 cycles of 15 sec at 94°C, 1 min at 55°C, and 3 min at 68°C and a final extension of 7 min at 68°C. .. PCR were performed in parallel reactions with 6 × 100 ng of template for each sample.

    Polymerase Chain Reaction:

    Article Title: Haploinsufficiency for NR3C1, the gene encoding the glucocorticoid receptor, in blastic plasmacytoid dendritic cell neoplasms
    Article Snippet: .. Long-distance PCR (LD-PCR) cloning of the t(3;5)(q21;q31) genomic breakpoint was performed using the Expand Long Template PCR system (Sigma) and LD-PCR primers, TA cloning of PCR products, and sequencing (supplemental Table 6). .. For RNA analysis, total RNA was prepared by TRIzol reagent, according to the manufacturer’s instructions.

    Article Title: Clock-dependent chromatin topology modulates circadian transcription and behavior
    Article Snippet: .. Six-hundred nanograms of 4C template was used for PCR amplification using Sigma-Aldrich long-template PCR system with bait-specific inverse primers conjugated to Illumina sequencing adaptors (primer sequences are in ) in a final volume of 50 µL in the following PCR program: 2 min at 94°C followed by 30 cycles of 15 sec at 94°C, 1 min at 55°C, and 3 min at 68°C and a final extension of 7 min at 68°C. .. PCR were performed in parallel reactions with 6 × 100 ng of template for each sample.

    Article Title: A CRISPR-Cas9 screen identifies essential CTCF anchor sites for estrogen receptor-driven breast cancer cell proliferation
    Article Snippet: .. 4C PCR was done using Expand long template polymerase (Sigma-Aldrich) in five separate PCR reactions using 100 ng per PCR. .. After Illumina sequencing, reads were demultiplexed based on the primer sequence of the viewpoint (first 20 nt of the sequence read).

    Article Title: 53BP1 Supports Immunoglobulin Class Switch Recombination Independently of Its DNA Double-Strand Break End Protection Function
    Article Snippet: .. Genomic DNA was extracted from the sorted population, and Sμ-Sα junctions were amplified by Expand long template PCR system (Sigma Aldrich). ..

    Article Title: NSD2 overexpression drives clustered chromatin and transcriptional changes in a subset of insulated domains
    Article Snippet: .. PCR was performed using Expand™ Long Template PCR System (Sigma) with the following thermocycler program: 94 °C for 2 min; 94 °C for 15 s; 53–55 °C for 1 min; 68 °C for 2.30 min; repeat for 29 cycles; 68 °C for 7 min; hold at 4 °C. .. 4C-seq libraries were size-selected on gel to remove any potential primer dimers and fragments above 700 bp, then quantified using RT-PCR (KAPA Biosystems) and sequenced using 50bp single-end on Illumina HiSeq 2500.

    Article Title: HSP90 inhibition targets autophagy and induces a CASP9-dependent resistance mechanism in NSCLC
    Article Snippet: .. PCR was performed using the Expand Long Template PCR System (Sigma, 11681834001) with variable PCR cycles depending on the primers utilized: ATG7 (27 cycles), CASP9 (23 cycles), SQSTM1 (25 cycles) and B2M (21 cycles). ..

    Article Title: Developing a tetO/TetR system in Neurospora crassa.
    Article Snippet: .. The development of a tetO/TetR system in the fungus Neurospora crassa is described. .. The development of a tetO/TetR system in the fungus Neurospora crassa is described.

    Article Title: NanoSatellite: accurate characterization of expanded tandem repeat length and sequence through whole genome long-read sequencing on PromethION
    Article Snippet: .. PCR optimizations for all TRs were performed with the Expand Long Template PCR System (Sigma-Aldrich, St. Louis, USA) and LongAmp Taq (NEB), with and without the addition of betain, at annealing temperatures ranging from 45 to 65 °C and with sufficiently long elongation times to support efficient amplification of long DNA sequences. .. To confirm specificity, the PCR amplicons were Sanger sequenced using BigDye reagents (Thermo Fisher Scientific) on a 3730 DNA analyzer (Thermo Fisher Scientific).

    Sequencing:

    Article Title: Haploinsufficiency for NR3C1, the gene encoding the glucocorticoid receptor, in blastic plasmacytoid dendritic cell neoplasms
    Article Snippet: .. Long-distance PCR (LD-PCR) cloning of the t(3;5)(q21;q31) genomic breakpoint was performed using the Expand Long Template PCR system (Sigma) and LD-PCR primers, TA cloning of PCR products, and sequencing (supplemental Table 6). .. For RNA analysis, total RNA was prepared by TRIzol reagent, according to the manufacturer’s instructions.

    Article Title: Clock-dependent chromatin topology modulates circadian transcription and behavior
    Article Snippet: .. Six-hundred nanograms of 4C template was used for PCR amplification using Sigma-Aldrich long-template PCR system with bait-specific inverse primers conjugated to Illumina sequencing adaptors (primer sequences are in ) in a final volume of 50 µL in the following PCR program: 2 min at 94°C followed by 30 cycles of 15 sec at 94°C, 1 min at 55°C, and 3 min at 68°C and a final extension of 7 min at 68°C. .. PCR were performed in parallel reactions with 6 × 100 ng of template for each sample.

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  • 95
    Millipore human male genomic dna
    Methylation status of the rRNA promoter in the parental colorectal carcinoma cells HCT116 and its derivative knockout cell lines. The methylation status of each CpG dinucleotide spanning −158 to +29 bp of human rRNA promoter in HCT116 colorectal carcinoma cells and derivative lines with targeted disruption of DNMT1 , DNMT3b or both genes. The rRNA promoter region was amplified from bisulfite-treated genomic <t>DNA</t> and cloned. The 16–19 randomly selected clones from each sample were subjected to automated sequencing. Each row represents the sequence of an individual clone, whereas each column depicts the position of the CpG. The filled and open circles denote methylated and unmethylated CpGs, respectively. The top row shows a heat map of methylated CpGs representing the frequency of methylation in each of the individual clones shown below. The degree of hypermethylation (NIM) at three CpG islands was assessed using quantitative real-time methylation-specific <t>PCR</t> and was normalized to the amount of bisulfite-converted input as described in ‘Materials and methods.'
    Human Male Genomic Dna, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human male genomic dna/product/Millipore
    Average 95 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    human male genomic dna - by Bioz Stars, 2020-05
    95/100 stars
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    Methylation status of the rRNA promoter in the parental colorectal carcinoma cells HCT116 and its derivative knockout cell lines. The methylation status of each CpG dinucleotide spanning −158 to +29 bp of human rRNA promoter in HCT116 colorectal carcinoma cells and derivative lines with targeted disruption of DNMT1 , DNMT3b or both genes. The rRNA promoter region was amplified from bisulfite-treated genomic DNA and cloned. The 16–19 randomly selected clones from each sample were subjected to automated sequencing. Each row represents the sequence of an individual clone, whereas each column depicts the position of the CpG. The filled and open circles denote methylated and unmethylated CpGs, respectively. The top row shows a heat map of methylated CpGs representing the frequency of methylation in each of the individual clones shown below. The degree of hypermethylation (NIM) at three CpG islands was assessed using quantitative real-time methylation-specific PCR and was normalized to the amount of bisulfite-converted input as described in ‘Materials and methods.'

    Journal: Oncogene

    Article Title: Overexpression of ribosomal RNA in prostate cancer is common but not linked to rDNA promoter hypomethylation

    doi: 10.1038/onc.2011.319

    Figure Lengend Snippet: Methylation status of the rRNA promoter in the parental colorectal carcinoma cells HCT116 and its derivative knockout cell lines. The methylation status of each CpG dinucleotide spanning −158 to +29 bp of human rRNA promoter in HCT116 colorectal carcinoma cells and derivative lines with targeted disruption of DNMT1 , DNMT3b or both genes. The rRNA promoter region was amplified from bisulfite-treated genomic DNA and cloned. The 16–19 randomly selected clones from each sample were subjected to automated sequencing. Each row represents the sequence of an individual clone, whereas each column depicts the position of the CpG. The filled and open circles denote methylated and unmethylated CpGs, respectively. The top row shows a heat map of methylated CpGs representing the frequency of methylation in each of the individual clones shown below. The degree of hypermethylation (NIM) at three CpG islands was assessed using quantitative real-time methylation-specific PCR and was normalized to the amount of bisulfite-converted input as described in ‘Materials and methods.'

    Article Snippet: PCR, genotyping and bisulfite genomic sequencing For initial PCR genotyping of the rDNA locus, 1 μl of human male genomic DNA (EMD Chemicals, Gibbstown, NJ, USA) was amplified by PCR using platinum Taq DNA polymerase (Invitrogen).

    Techniques: Methylation, Knock-Out, Amplification, Clone Assay, Sequencing, Polymerase Chain Reaction

    The human rDNA promoter region. ( a ) Genotyping of rDNA promoter region. The sequence of the rDNA promoter as shown was identical for all prostate cancer tissues and normal tissues, as well as all the cell lines. In this map, primer sequences for genotyping are indicated by the underlined regions and the primer sequences for bisulfite sequencing are indicated as dotted underlines. CpG dinucleotides are boxed with the location marked relative to the transcription start site (arrow). Discrepancies with the published DNA sequence (U13369) are marked. Nucleotides that show base substitutions as compared with the reference U13369 seqeunce are illustrated with dots above the DNA sequence. Deletions (generally a single base), relative to the reference sequence, are marked by a ‘v ‘between nucleotides. ( b ) Schematic representation of a single human rDNA repeat. The approximate positions relative to the transcription start site are indicated (DNA base number in kb). ( c ) Vertical lines indicate locations of CpG dinucleotides on the 45S rRNA promoter relative to the transcription start site, indicated with the dashed curved arrow, with primer pairs used for bisulfite mapping marked by inward pointing arrows.

    Journal: Oncogene

    Article Title: Overexpression of ribosomal RNA in prostate cancer is common but not linked to rDNA promoter hypomethylation

    doi: 10.1038/onc.2011.319

    Figure Lengend Snippet: The human rDNA promoter region. ( a ) Genotyping of rDNA promoter region. The sequence of the rDNA promoter as shown was identical for all prostate cancer tissues and normal tissues, as well as all the cell lines. In this map, primer sequences for genotyping are indicated by the underlined regions and the primer sequences for bisulfite sequencing are indicated as dotted underlines. CpG dinucleotides are boxed with the location marked relative to the transcription start site (arrow). Discrepancies with the published DNA sequence (U13369) are marked. Nucleotides that show base substitutions as compared with the reference U13369 seqeunce are illustrated with dots above the DNA sequence. Deletions (generally a single base), relative to the reference sequence, are marked by a ‘v ‘between nucleotides. ( b ) Schematic representation of a single human rDNA repeat. The approximate positions relative to the transcription start site are indicated (DNA base number in kb). ( c ) Vertical lines indicate locations of CpG dinucleotides on the 45S rRNA promoter relative to the transcription start site, indicated with the dashed curved arrow, with primer pairs used for bisulfite mapping marked by inward pointing arrows.

    Article Snippet: PCR, genotyping and bisulfite genomic sequencing For initial PCR genotyping of the rDNA locus, 1 μl of human male genomic DNA (EMD Chemicals, Gibbstown, NJ, USA) was amplified by PCR using platinum Taq DNA polymerase (Invitrogen).

    Techniques: Sequencing, Methylation Sequencing