long range pcr  (Qiagen)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    QIAGEN LongRange PCR Kit
    Description:
    For sensitive and accurate long range PCR Kit contents Qiagen LongRange PCR Kit 20 x 50L rxns Amplification of Extremely Long PCR Products up to 40 kb DNA Low Error Rates Ensured by High fidelity Enzyme Minimal PCR Optimization due to Unique Buffer System Amplification of Low copy Targets and GC rich Templates For Sensitive and Accurate Long range PCR Includes LongRange PCR Enzyme Mix 40U LongRange PCR Buffer 5x Q Solution RNase free Water 10mM dNTPs Benefits Amplification of extremely long PCR products up to 40 kb DNA Low error rates ensured by high fidelity enzyme Minimal PCR optimization due to unique buffer system Amplification of low copy targets and GC rich templates
    Catalog Number:
    206401
    Price:
    66.9
    Category:
    QIAGEN LongRange PCR Kit
    Buy from Supplier


    Structured Review

    Qiagen long range pcr
    QIAGEN LongRange PCR Kit
    For sensitive and accurate long range PCR Kit contents Qiagen LongRange PCR Kit 20 x 50L rxns Amplification of Extremely Long PCR Products up to 40 kb DNA Low Error Rates Ensured by High fidelity Enzyme Minimal PCR Optimization due to Unique Buffer System Amplification of Low copy Targets and GC rich Templates For Sensitive and Accurate Long range PCR Includes LongRange PCR Enzyme Mix 40U LongRange PCR Buffer 5x Q Solution RNase free Water 10mM dNTPs Benefits Amplification of extremely long PCR products up to 40 kb DNA Low error rates ensured by high fidelity enzyme Minimal PCR optimization due to unique buffer system Amplification of low copy targets and GC rich templates
    https://www.bioz.com/result/long range pcr/product/Qiagen
    Average 99 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    long range pcr - by Bioz Stars, 2020-07
    99/100 stars

    Images

    1) Product Images from "An entire exon 3 germ-line rearrangement in the BRCA2 gene: pathogenic relevance of exon 3 deletion in breast cancer predisposition"

    Article Title: An entire exon 3 germ-line rearrangement in the BRCA2 gene: pathogenic relevance of exon 3 deletion in breast cancer predisposition

    Journal: BMC Medical Genetics

    doi: 10.1186/1471-2350-12-121

    Genomic analysis of the Δ3 BRCA2 large rearrangement . a: Dedicated BRCA2 CGH array . The gene is represented at the top, with vertical boxes that indicate exon positions and sizes. Black plots are considered to be within the diploidy range (the y axis gives the log2 intensity ratios). The green dots indicate signals that were below the threshold for deletion (-0.4 log2 ratio). b: Sequence analysis of the smaller PCR product obtained by long-range PCR of proband DNA with the exon 3 large rearrangement in BRCA2 (Hg18/build36, 2006). The sequence crosses the breakpoint that begins in intron 2 and ends in intron 3.
    Figure Legend Snippet: Genomic analysis of the Δ3 BRCA2 large rearrangement . a: Dedicated BRCA2 CGH array . The gene is represented at the top, with vertical boxes that indicate exon positions and sizes. Black plots are considered to be within the diploidy range (the y axis gives the log2 intensity ratios). The green dots indicate signals that were below the threshold for deletion (-0.4 log2 ratio). b: Sequence analysis of the smaller PCR product obtained by long-range PCR of proband DNA with the exon 3 large rearrangement in BRCA2 (Hg18/build36, 2006). The sequence crosses the breakpoint that begins in intron 2 and ends in intron 3.

    Techniques Used: Sequencing, Polymerase Chain Reaction

    2) Product Images from "Exome sequencing resolves apparent incidental findings and reveals further complexity of SH3TC2 variant alleles causing Charcot-Marie-Tooth neuropathy"

    Article Title: Exome sequencing resolves apparent incidental findings and reveals further complexity of SH3TC2 variant alleles causing Charcot-Marie-Tooth neuropathy

    Journal: Genome Medicine

    doi: 10.1186/gm461

    ( a ) Genomic landscape of the region containing the ABCD1 gene and the repeated 9 .7 kb segment reported as a partial pseudogene. This segment comprises exons 7 to 10 of the ABCD1 gene and is repeated in four other locations in the reference genome with approximately 95% identity. ( b ) Comparison of Sanger sequencing of the reported mutation (p.Gly608Asp) through direct PCR of the segment containing exon 8 and 9 of ABCD1 using genomic DNA as a template (upper) and nested PCR of the same segment after long-range PCR amplification of an approximately 10 kb segment using primers specific to the ABCD1 locus outside of the repeated segment (lower).
    Figure Legend Snippet: ( a ) Genomic landscape of the region containing the ABCD1 gene and the repeated 9 .7 kb segment reported as a partial pseudogene. This segment comprises exons 7 to 10 of the ABCD1 gene and is repeated in four other locations in the reference genome with approximately 95% identity. ( b ) Comparison of Sanger sequencing of the reported mutation (p.Gly608Asp) through direct PCR of the segment containing exon 8 and 9 of ABCD1 using genomic DNA as a template (upper) and nested PCR of the same segment after long-range PCR amplification of an approximately 10 kb segment using primers specific to the ABCD1 locus outside of the repeated segment (lower).

    Techniques Used: Sequencing, Mutagenesis, Polymerase Chain Reaction, Nested PCR, Amplification

    3) Product Images from "Paradoxical effects of repeat interruptions on spinocerebellar ataxia type 10 expansions and repeat instability"

    Article Title: Paradoxical effects of repeat interruptions on spinocerebellar ataxia type 10 expansions and repeat instability

    Journal: European Journal of Human Genetics

    doi: 10.1038/ejhg.2013.32

    Configuration of ATCCT repeat interruptions within the SCA10 pentanucleotide expansion. ( a ) Schematic of the SCA10 repeat expansion (red rectangle) in intron 9 of the Ataxin 10 gene, depicting the 5′- and 3′-ends of the repeat. Primers used to amplify full-length (LR-F and LR-R) and ATCCT-PCR (LP-L and L2RT) products are indicated by arrows relative to the repeat. ( b ) Schematic of the motif structure of the 5′-end of the expansion obtained from SCA10 hybrid cell lines. Sequence obtained is as follows: 5′-ATTCT 40–41 ATTTTCT ATTCT (ATATTCT ATTCT) 2 ATTTTCT ATTCT 11 ATATTCT ATTCT 5 ATATTCT ATTCT 10 ATATTCT ATTCT 2 ATATTCT ATTCT 12 (ATATTCT ATTCT) 2 ATTCT 11 (ATATTCT ATTCT) 4 ATTCT 2 ATATTCT ATTCT 8–10 (ATATTCT ATTCT) 5 ATTCT 7–8 ATATTCT ATTCT n ( c ) Schematic of the motif structure of the 3′-end of the expansion obtained from SCA10 hybrid lines. Sequence obtained is as follows: 5′-(ATCCT) 34+ (ATCCC) 4–28 (ATCCT) 2 ATTCT 1 -3′. ( d ) Typical ATCCT-PCR results from affected (+) or unaffected (−) samples of Mexican (M) or Brazilian (B) ancestry. Lanes containing no template control (ntc) or DNA size standard (L) are indicated. The arrow indicates the 1.2-kb ATCCT-PCR product size. The faint large (∼2.2 kb) band may be a product that resulted from annealing of the reverse primer to interrupted sequence further downstream, whereas the faint middle band is a constant background band found even in unaffected individuals. ( e ) An example of sequence obtained by direct sequencing of 1.2-kb band of the ATCCT-positive PCR product (from lane 1 above; 5′-ATTCT 30+ ATTTTCT ATTCT (ATATTCT ATTCT) 2 ATTTTCT ATTCT 11 ATATTCT ATTCT 5 ATATTCT ATTCT 10 ATATTCT ATTCT 2 ATATTCT ATTCT 12 ATATTCT ATTCT ATATTCT ATTCT 12 (ATATTCT ATTCT) 4 ATTCT 2 ATATTCT ATTCT 10 (ATATTCT ATTCT) 5 ATTCT 7 ATATTCT ATTCT 14 ATATTCT ATTCT 3 ATATTCT ATTCT 16 (ATCCT) 2+ -3′). An exact count of ATTCT repeats at the 5′-end cannot be made as the sequencing primer anneals near the beginning of the repeat and sequence data began with ATTCT sequences. However, at least 30–35 repeats were observed in various samples. In panels b , c and e : white rectangle, ATTCT motif; orange rectangle, ATTTTCT interruption motif; blue rectangle, ATATTCT interruption motif; gray rectangle, ATCCT interruption motif; green rectangle, ATCCC interruption motif as indicated.
    Figure Legend Snippet: Configuration of ATCCT repeat interruptions within the SCA10 pentanucleotide expansion. ( a ) Schematic of the SCA10 repeat expansion (red rectangle) in intron 9 of the Ataxin 10 gene, depicting the 5′- and 3′-ends of the repeat. Primers used to amplify full-length (LR-F and LR-R) and ATCCT-PCR (LP-L and L2RT) products are indicated by arrows relative to the repeat. ( b ) Schematic of the motif structure of the 5′-end of the expansion obtained from SCA10 hybrid cell lines. Sequence obtained is as follows: 5′-ATTCT 40–41 ATTTTCT ATTCT (ATATTCT ATTCT) 2 ATTTTCT ATTCT 11 ATATTCT ATTCT 5 ATATTCT ATTCT 10 ATATTCT ATTCT 2 ATATTCT ATTCT 12 (ATATTCT ATTCT) 2 ATTCT 11 (ATATTCT ATTCT) 4 ATTCT 2 ATATTCT ATTCT 8–10 (ATATTCT ATTCT) 5 ATTCT 7–8 ATATTCT ATTCT n ( c ) Schematic of the motif structure of the 3′-end of the expansion obtained from SCA10 hybrid lines. Sequence obtained is as follows: 5′-(ATCCT) 34+ (ATCCC) 4–28 (ATCCT) 2 ATTCT 1 -3′. ( d ) Typical ATCCT-PCR results from affected (+) or unaffected (−) samples of Mexican (M) or Brazilian (B) ancestry. Lanes containing no template control (ntc) or DNA size standard (L) are indicated. The arrow indicates the 1.2-kb ATCCT-PCR product size. The faint large (∼2.2 kb) band may be a product that resulted from annealing of the reverse primer to interrupted sequence further downstream, whereas the faint middle band is a constant background band found even in unaffected individuals. ( e ) An example of sequence obtained by direct sequencing of 1.2-kb band of the ATCCT-positive PCR product (from lane 1 above; 5′-ATTCT 30+ ATTTTCT ATTCT (ATATTCT ATTCT) 2 ATTTTCT ATTCT 11 ATATTCT ATTCT 5 ATATTCT ATTCT 10 ATATTCT ATTCT 2 ATATTCT ATTCT 12 ATATTCT ATTCT ATATTCT ATTCT 12 (ATATTCT ATTCT) 4 ATTCT 2 ATATTCT ATTCT 10 (ATATTCT ATTCT) 5 ATTCT 7 ATATTCT ATTCT 14 ATATTCT ATTCT 3 ATATTCT ATTCT 16 (ATCCT) 2+ -3′). An exact count of ATTCT repeats at the 5′-end cannot be made as the sequencing primer anneals near the beginning of the repeat and sequence data began with ATTCT sequences. However, at least 30–35 repeats were observed in various samples. In panels b , c and e : white rectangle, ATTCT motif; orange rectangle, ATTTTCT interruption motif; blue rectangle, ATATTCT interruption motif; gray rectangle, ATCCT interruption motif; green rectangle, ATCCC interruption motif as indicated.

    Techniques Used: Polymerase Chain Reaction, Sequencing

    4) Product Images from "New Fusion Transcripts Identified in Normal Karyotype Acute Myeloid Leukemia"

    Article Title: New Fusion Transcripts Identified in Normal Karyotype Acute Myeloid Leukemia

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0051203

    Validation of sense and antisense fusion transcripts by strand-specific RT-PCR. A. Summary for RNA samples from 8 myeloid cell lines. B. CIITA-DEXI sense and antisense fusion transcripts detected in myeloid cell lines and AML samples. +: positive control with beta-actin; -: netative control without RNA templates.
    Figure Legend Snippet: Validation of sense and antisense fusion transcripts by strand-specific RT-PCR. A. Summary for RNA samples from 8 myeloid cell lines. B. CIITA-DEXI sense and antisense fusion transcripts detected in myeloid cell lines and AML samples. +: positive control with beta-actin; -: netative control without RNA templates.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Positive Control

    5) Product Images from "The complexity of Rhipicephalus (Boophilus) microplus genome characterised through detailed analysis of two BAC clones"

    Article Title: The complexity of Rhipicephalus (Boophilus) microplus genome characterised through detailed analysis of two BAC clones

    Journal: BMC Research Notes

    doi: 10.1186/1756-0500-4-254

    BAC BM-012-E08 50 Kb sequence, with a repetitive 8 Kb intergenic spacer (brown), and rRNA 18S, ITS1, 5.8S, ITS2 and 28S (blue) together make the repeat unit structure (light blue) . The BES start position are indicated by a green arrow and *copies. Below is BAC BM-012-E08 sequence dot matrix that displays the highly repetitive regions and rRNA (blue) fragments. On the horizontal axis regions confirmed (red) by PCR and not confirmed (yellow) are shown. Displayed below the dot matrix are the mapped Cot696 reads.
    Figure Legend Snippet: BAC BM-012-E08 50 Kb sequence, with a repetitive 8 Kb intergenic spacer (brown), and rRNA 18S, ITS1, 5.8S, ITS2 and 28S (blue) together make the repeat unit structure (light blue) . The BES start position are indicated by a green arrow and *copies. Below is BAC BM-012-E08 sequence dot matrix that displays the highly repetitive regions and rRNA (blue) fragments. On the horizontal axis regions confirmed (red) by PCR and not confirmed (yellow) are shown. Displayed below the dot matrix are the mapped Cot696 reads.

    Techniques Used: BAC Assay, Sequencing, Polymerase Chain Reaction

    6) Product Images from "The neuronal K+Cl− co-transporter 2 (Slc12a5) modulates insulin secretion"

    Article Title: The neuronal K+Cl− co-transporter 2 (Slc12a5) modulates insulin secretion

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-01814-0

    KCC2 is expressed in rodent β-cell lines and pancreatic islets. ( A – D ) Original ethidium bromide stained gels (inverted) showing KCC2 mRNA expression in the mouse β-cell line MIN6 ( A ), human ( B ) and mouse islets ( C ) and the rat INS1-E β-cell line ( D ). Shown are PCR products of expected sizes representing KCC2a and KCC2b. As the positive control of RT-PCRs, transcripts of GAPDH were amplified (555 bp). As the negative control, water was used instead of total cDNA. ( E ) Partial chromatograms obtained from representative DNA sequencing reactions using purified KCC2a-671 and KCC2b-602 PCR products from MIN6 ( black asterisks ). The DNA sequences obtained are 100% identical to the spliced version of KCC2a and KCC2b, respectively. ( F ) Representative original 1% agarose gel digital image cropped to include bands of relevant sizes. RT coupled to long-range PCR using total RNA from MIN6 and primer sets KCC2a-3591 or KCC2b-4024 (Supplementary Table 1 ). The PCR products of 3591 bp (KCC2a) and 4024 bp (KCC2b) were purified from this gel, directly cloned in pCR-Blunt II-TOPO vectors and fully sequenced in both directions. ( G ) MIN6 and mouse brain KCC2a and KCC2b mRNA quantification ( n = 5). Results are expressed relative to MIN6 KCC2a. ( H ) Cropped digitised immunoblots of the indicated protein extracts (µg) obtained from MIN6, mouse (m) and human (h) islets and mouse brain. ( I ) Digitized immunoblot cropped to show KCC2 protein expression in purified plasma membranes of COS7 cells and MIN6 β-cells. ( J,K ) Confocal images of MIN6 β-cells immunolabeled using validated KCC2 antibodies. The square in ( J ) is shown at higher magnification in K to indicate with white arrows immunoreactive KCC2 towards the edges of the cells. Nuclei have been counterstained with DAPI. Scale bar represents 10 µm.
    Figure Legend Snippet: KCC2 is expressed in rodent β-cell lines and pancreatic islets. ( A – D ) Original ethidium bromide stained gels (inverted) showing KCC2 mRNA expression in the mouse β-cell line MIN6 ( A ), human ( B ) and mouse islets ( C ) and the rat INS1-E β-cell line ( D ). Shown are PCR products of expected sizes representing KCC2a and KCC2b. As the positive control of RT-PCRs, transcripts of GAPDH were amplified (555 bp). As the negative control, water was used instead of total cDNA. ( E ) Partial chromatograms obtained from representative DNA sequencing reactions using purified KCC2a-671 and KCC2b-602 PCR products from MIN6 ( black asterisks ). The DNA sequences obtained are 100% identical to the spliced version of KCC2a and KCC2b, respectively. ( F ) Representative original 1% agarose gel digital image cropped to include bands of relevant sizes. RT coupled to long-range PCR using total RNA from MIN6 and primer sets KCC2a-3591 or KCC2b-4024 (Supplementary Table 1 ). The PCR products of 3591 bp (KCC2a) and 4024 bp (KCC2b) were purified from this gel, directly cloned in pCR-Blunt II-TOPO vectors and fully sequenced in both directions. ( G ) MIN6 and mouse brain KCC2a and KCC2b mRNA quantification ( n = 5). Results are expressed relative to MIN6 KCC2a. ( H ) Cropped digitised immunoblots of the indicated protein extracts (µg) obtained from MIN6, mouse (m) and human (h) islets and mouse brain. ( I ) Digitized immunoblot cropped to show KCC2 protein expression in purified plasma membranes of COS7 cells and MIN6 β-cells. ( J,K ) Confocal images of MIN6 β-cells immunolabeled using validated KCC2 antibodies. The square in ( J ) is shown at higher magnification in K to indicate with white arrows immunoreactive KCC2 towards the edges of the cells. Nuclei have been counterstained with DAPI. Scale bar represents 10 µm.

    Techniques Used: Staining, Expressing, Polymerase Chain Reaction, Positive Control, Amplification, Negative Control, DNA Sequencing, Purification, Agarose Gel Electrophoresis, Clone Assay, Western Blot, Immunolabeling

    KCC2-S25 is expressed in MIN6 β-cells, human islets and mouse pancreas. ( A ) Representation of KCC2a/b amplicons obtained by using the KCC2-565 primer set. Indicated are the MspI restriction sites and the predicted length of the digestion products in bp. Exon 25 is highlighted in red. Its splicing eliminates an MspI site in the amplicon. ( B ) Ethidium bormide stained gel, inverted from its original gray-scale digital picture, showing RT-PCR products of expected size (565 bp) obtained by using the primer set KCC2-565 and total RNA from mouse spinal cord, brain and MIN6 β-cells. As negative control, water was used instead of total cDNA. ( C ) Representative ethidium bromide stained 2% agarose gel inverted from original where MspI -digested PCR products were separated. Also shown, representative densitometry analysis of the MspI banding pattern to estimate the relative contribution of KCC2-S25 (~54%) to the total KCC2 pool. ( D ) Representative ethidium bromide stained gel inverted from original showing an RT-PCR experiment performed using mouse islet RNA and the KCC2-565 primer set. Note the product of expected size and MspI digestion analysis of restriction fragments. ( E ) Representative ethidium bormide stained gel inverted from original showing RT-PCR experiment using total RNA from human islets and the KCC2-657 primer to obtain amplicons of expected size (657 bp) and a posteriori MspI digestion analysis. ( F ) Expression levels of total KCC2 in adult mouse brain using qPCR primers that do not distinguish among known KCC2 variants (total KCC2) or specific to exon 25 (KCC2a/b). ( G ) Representation of human KCC2a/b amplicons obtained using KCC2-657 primer set and predicted MspI restriction fragments for KCC2a/b-S25 (176 bp) and KCC2a/b (102 bp + 89 bp).
    Figure Legend Snippet: KCC2-S25 is expressed in MIN6 β-cells, human islets and mouse pancreas. ( A ) Representation of KCC2a/b amplicons obtained by using the KCC2-565 primer set. Indicated are the MspI restriction sites and the predicted length of the digestion products in bp. Exon 25 is highlighted in red. Its splicing eliminates an MspI site in the amplicon. ( B ) Ethidium bormide stained gel, inverted from its original gray-scale digital picture, showing RT-PCR products of expected size (565 bp) obtained by using the primer set KCC2-565 and total RNA from mouse spinal cord, brain and MIN6 β-cells. As negative control, water was used instead of total cDNA. ( C ) Representative ethidium bromide stained 2% agarose gel inverted from original where MspI -digested PCR products were separated. Also shown, representative densitometry analysis of the MspI banding pattern to estimate the relative contribution of KCC2-S25 (~54%) to the total KCC2 pool. ( D ) Representative ethidium bromide stained gel inverted from original showing an RT-PCR experiment performed using mouse islet RNA and the KCC2-565 primer set. Note the product of expected size and MspI digestion analysis of restriction fragments. ( E ) Representative ethidium bormide stained gel inverted from original showing RT-PCR experiment using total RNA from human islets and the KCC2-657 primer to obtain amplicons of expected size (657 bp) and a posteriori MspI digestion analysis. ( F ) Expression levels of total KCC2 in adult mouse brain using qPCR primers that do not distinguish among known KCC2 variants (total KCC2) or specific to exon 25 (KCC2a/b). ( G ) Representation of human KCC2a/b amplicons obtained using KCC2-657 primer set and predicted MspI restriction fragments for KCC2a/b-S25 (176 bp) and KCC2a/b (102 bp + 89 bp).

    Techniques Used: Amplification, Staining, Reverse Transcription Polymerase Chain Reaction, Negative Control, Agarose Gel Electrophoresis, Polymerase Chain Reaction, Expressing, Real-time Polymerase Chain Reaction

    7) Product Images from "Complete Mitochondrial Genome and Phylogeny of Pleistocene Mammoth Mammuthus primigeniusReading the Evolutionary History of the Woolly Mammoth in Its Mitochondrial GenomeThe Year of the Mammoth"

    Article Title: Complete Mitochondrial Genome and Phylogeny of Pleistocene Mammoth Mammuthus primigeniusReading the Evolutionary History of the Woolly Mammoth in Its Mitochondrial GenomeThe Year of the Mammoth

    Journal: PLoS Biology

    doi: 10.1371/journal.pbio.0040073

    The Unusually Well-Preserved Mammoth DNA (A) Nuclei with DNA clearly detectable by DAPI staining in muscle cells of ~33,000-y-old M. primigenius . (B) Total genomic DNA isolated from the mammoth muscle tissue (lane 1 is 1/10 dilution of the DNA on lane 2); control DNA from fresh human blood samples in lanes 3 and 4. (C) Examples of PCR products (~300–600 bp) for mammoth mitochondrial genome. (D) PCR amplification recovers long sequences for complete mitochondrial genes (1,317 bp CytB and 1,613 bp ATP6 genes), but PCR of larger fragments (3,054 bp ND5 ) is failed.
    Figure Legend Snippet: The Unusually Well-Preserved Mammoth DNA (A) Nuclei with DNA clearly detectable by DAPI staining in muscle cells of ~33,000-y-old M. primigenius . (B) Total genomic DNA isolated from the mammoth muscle tissue (lane 1 is 1/10 dilution of the DNA on lane 2); control DNA from fresh human blood samples in lanes 3 and 4. (C) Examples of PCR products (~300–600 bp) for mammoth mitochondrial genome. (D) PCR amplification recovers long sequences for complete mitochondrial genes (1,317 bp CytB and 1,613 bp ATP6 genes), but PCR of larger fragments (3,054 bp ND5 ) is failed.

    Techniques Used: Staining, Isolation, Polymerase Chain Reaction, Amplification

    Related Articles

    Polymerase Chain Reaction:

    Article Title: An entire exon 3 germ-line rearrangement in the BRCA2 gene: pathogenic relevance of exon 3 deletion in breast cancer predisposition
    Article Snippet: .. DNA breakpoints analysis The breakpoints were characterized by long-range PCR of genomic DNA using the Qiagen Long-Range PCR kit (Qiagen, Germany). .. 150 ng of DNA was amplified with a primer pair that tightly flanks the respective breakpoint regions identified by the CGH-array in a reaction volume of 25 μl (Table ).

    Article Title: Preimplantation High-Resolution HLA Sequencing Using Next Generation Sequencing.
    Article Snippet: .. The kit provides the LongRange PCR Kit (Qiagen, Hilden, Germany) and multiple primers for the 5 HLA loci including HLA-A, -B, -C, -DRB1, and -DQB1. ..

    Article Title: Identification of Variants of Hepatitis C Virus (HCV) Entry Factors in Patients Highly Exposed to HCV but Remaining Uninfected: An ANRS Case-Control Study
    Article Snippet: .. To avoid amplification of the pseudogene for exons 6 to 9 of the OCLN gene, long-range (LR) PCR (Qiagen LongRange PCR Kit) was used as an intermediate step before PCR amplification of the exon. .. LR PCR amplification consisted of 10 min at 94°C followed by 30 cycles of 20 s at 94°C, 1 min at 68°C and 5 min at 72°C, and a final 10-min elongation step at 72°C.

    Article Title: Elevated expression of a minor isoform of ANK3 is a risk factor for bipolar disorder
    Article Snippet: .. Target-specific products were generated directly from first-strand cDNA products, using the QIAGEN LongRange PCR Kit (QIAGEN, Hilden, Germany) and four different forward primers placed in each of the four first exons of ANK3 (Fig. ) and one common reverse primer in the 3’ UTR. .. Finally, peak detection was performed with the Agilent DNA 12000 Kit on the 2100 Bioanalyzer system (Agilent Technologies, Santa Clara, CA, USA).

    Article Title: ZFP36-FOSB Fusion Defines a Subset of Epithelioid Hemangioma with Atypical Features
    Article Snippet: .. Additionally for the WWTR1-FOSB intronic break Long-Range DNA was carried out by using QIAGEN LongRange PCR Kit (QIAGEN, Germantown, MD), from 1 μg of genomic DNA. ..

    Article Title: EWSR1 fusions with CREB family transcription factors define a novel myxoid mesenchymal tumor with predilection for intracranial location
    Article Snippet: .. Long range PCR was carried out using QIAGEN LongRange PCR Kit (QIAGEN, Germantown, MD). ..

    Article Title: A Single Nucleotide Polymorphism Associated with Hepatitis C Virus Infections Located in the Distal Region of the IL28B Promoter Influences NF-?B-Mediated Gene Transcription
    Article Snippet: .. The SNP rs28416813 was amplified with the Long-range PCR kit from Qiagen by using: Forward primer 1.9kbrs813KpnIFor2 (5′GATATCGGTACCTGCATTGTACGACCCTCCAAC-3′) and reverse primer: 1.9kbil28b12aaHIIIREV1 (5′GATATCAAGCTTCAGCACTGCGGCCATCAG-3′). .. Due to high sequence homology between IL28A and IL28B the primers gave rise to two amplicons of sizes 2673 bp corresponding to promoter of IL28A and 1975 bp corresponding to that of IL28B .

    Article Title: A deletion of the HBII-85 class of small nucleolar RNAs (snoRNAs) is associated with hyperphagia, obesity and hypogonadism
    Article Snippet: .. Long-range PCR was carried out on DNA from both the patient and the patient's father, using the Qiagen LongRange PCR Kit (see manufacturers protocol for details). ..

    Generated:

    Article Title: Elevated expression of a minor isoform of ANK3 is a risk factor for bipolar disorder
    Article Snippet: .. Target-specific products were generated directly from first-strand cDNA products, using the QIAGEN LongRange PCR Kit (QIAGEN, Hilden, Germany) and four different forward primers placed in each of the four first exons of ANK3 (Fig. ) and one common reverse primer in the 3’ UTR. .. Finally, peak detection was performed with the Agilent DNA 12000 Kit on the 2100 Bioanalyzer system (Agilent Technologies, Santa Clara, CA, USA).

    Amplification:

    Article Title: Identification of Variants of Hepatitis C Virus (HCV) Entry Factors in Patients Highly Exposed to HCV but Remaining Uninfected: An ANRS Case-Control Study
    Article Snippet: .. To avoid amplification of the pseudogene for exons 6 to 9 of the OCLN gene, long-range (LR) PCR (Qiagen LongRange PCR Kit) was used as an intermediate step before PCR amplification of the exon. .. LR PCR amplification consisted of 10 min at 94°C followed by 30 cycles of 20 s at 94°C, 1 min at 68°C and 5 min at 72°C, and a final 10-min elongation step at 72°C.

    Article Title: A Single Nucleotide Polymorphism Associated with Hepatitis C Virus Infections Located in the Distal Region of the IL28B Promoter Influences NF-?B-Mediated Gene Transcription
    Article Snippet: .. The SNP rs28416813 was amplified with the Long-range PCR kit from Qiagen by using: Forward primer 1.9kbrs813KpnIFor2 (5′GATATCGGTACCTGCATTGTACGACCCTCCAAC-3′) and reverse primer: 1.9kbil28b12aaHIIIREV1 (5′GATATCAAGCTTCAGCACTGCGGCCATCAG-3′). .. Due to high sequence homology between IL28A and IL28B the primers gave rise to two amplicons of sizes 2673 bp corresponding to promoter of IL28A and 1975 bp corresponding to that of IL28B .

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Qiagen long range pcr
    Genomic analysis of the Δ3 BRCA2 large rearrangement . a: Dedicated BRCA2 CGH array . The gene is represented at the top, with vertical boxes that indicate exon positions and sizes. Black plots are considered to be within the diploidy range (the y axis gives the log2 intensity ratios). The green dots indicate signals that were below the threshold for deletion (-0.4 log2 ratio). b: Sequence analysis of the smaller <t>PCR</t> product obtained by long-range PCR of proband <t>DNA</t> with the exon 3 large rearrangement in BRCA2 (Hg18/build36, 2006). The sequence crosses the breakpoint that begins in intron 2 and ends in intron 3.
    Long Range Pcr, supplied by Qiagen, used in various techniques. Bioz Stars score: 92/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/long range pcr/product/Qiagen
    Average 92 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    long range pcr - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    99
    Qiagen long range rt pcr kit
    Characterization of ectopically expressed RNAs by long range <t>RT-PCR.</t> MDA-MB-231 parental cell line (231-parental), its pcDNA3-ERα stable transfectant (ERα-2), and its ERα-IRES stable transfectants (ERα-IRES-5 and ERα-IRES-3) were analyzed for expression of ERα–harboring transcript (1.8 kb), Hygromycin B resistance gene-containing transcript (1.0 kb), and ERα-IRES-Hygro R fused transcript (3.2 kb), by RT followed by long range PCR amplification. pERα-IRES DNA served as a PCR positive control for the ERα <t>cDNA</t> primers (1.8 kb), the Hygromycin B resistance gene ORF primers (1.0 kb), and the 5′ sense ERα primer plus 3′ antisense Hygro R fused ORFs primers (3.5 kb). A First four lanes from left contain the ERα cDNA primers; lanes 5–8 the 5′ sense ERα primer together with the 3′ antisense Hygro R gene primer. B. The 5′ sense ERα primer together with the 3′ antisense Hygro R gene primer. C. Lanes 1 and 2 from left, the ERα primers. Lanes 3 and 4 the Hygro R gene primers. Primer sequences are detailed in the “ Methods ” section.
    Long Range Rt Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/long range rt pcr kit/product/Qiagen
    Average 99 stars, based on 49 article reviews
    Price from $9.99 to $1999.99
    long range rt pcr kit - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    Genomic analysis of the Δ3 BRCA2 large rearrangement . a: Dedicated BRCA2 CGH array . The gene is represented at the top, with vertical boxes that indicate exon positions and sizes. Black plots are considered to be within the diploidy range (the y axis gives the log2 intensity ratios). The green dots indicate signals that were below the threshold for deletion (-0.4 log2 ratio). b: Sequence analysis of the smaller PCR product obtained by long-range PCR of proband DNA with the exon 3 large rearrangement in BRCA2 (Hg18/build36, 2006). The sequence crosses the breakpoint that begins in intron 2 and ends in intron 3.

    Journal: BMC Medical Genetics

    Article Title: An entire exon 3 germ-line rearrangement in the BRCA2 gene: pathogenic relevance of exon 3 deletion in breast cancer predisposition

    doi: 10.1186/1471-2350-12-121

    Figure Lengend Snippet: Genomic analysis of the Δ3 BRCA2 large rearrangement . a: Dedicated BRCA2 CGH array . The gene is represented at the top, with vertical boxes that indicate exon positions and sizes. Black plots are considered to be within the diploidy range (the y axis gives the log2 intensity ratios). The green dots indicate signals that were below the threshold for deletion (-0.4 log2 ratio). b: Sequence analysis of the smaller PCR product obtained by long-range PCR of proband DNA with the exon 3 large rearrangement in BRCA2 (Hg18/build36, 2006). The sequence crosses the breakpoint that begins in intron 2 and ends in intron 3.

    Article Snippet: DNA breakpoints analysis The breakpoints were characterized by long-range PCR of genomic DNA using the Qiagen Long-Range PCR kit (Qiagen, Germany).

    Techniques: Sequencing, Polymerase Chain Reaction

    Characterization of ectopically expressed RNAs by long range RT-PCR. MDA-MB-231 parental cell line (231-parental), its pcDNA3-ERα stable transfectant (ERα-2), and its ERα-IRES stable transfectants (ERα-IRES-5 and ERα-IRES-3) were analyzed for expression of ERα–harboring transcript (1.8 kb), Hygromycin B resistance gene-containing transcript (1.0 kb), and ERα-IRES-Hygro R fused transcript (3.2 kb), by RT followed by long range PCR amplification. pERα-IRES DNA served as a PCR positive control for the ERα cDNA primers (1.8 kb), the Hygromycin B resistance gene ORF primers (1.0 kb), and the 5′ sense ERα primer plus 3′ antisense Hygro R fused ORFs primers (3.5 kb). A First four lanes from left contain the ERα cDNA primers; lanes 5–8 the 5′ sense ERα primer together with the 3′ antisense Hygro R gene primer. B. The 5′ sense ERα primer together with the 3′ antisense Hygro R gene primer. C. Lanes 1 and 2 from left, the ERα primers. Lanes 3 and 4 the Hygro R gene primers. Primer sequences are detailed in the “ Methods ” section.

    Journal: PLoS ONE

    Article Title: ER-Alpha-cDNA As Part of a Bicistronic Transcript Gives Rise to High Frequency, Long Term, Receptor Expressing Cell Clones

    doi: 10.1371/journal.pone.0031977

    Figure Lengend Snippet: Characterization of ectopically expressed RNAs by long range RT-PCR. MDA-MB-231 parental cell line (231-parental), its pcDNA3-ERα stable transfectant (ERα-2), and its ERα-IRES stable transfectants (ERα-IRES-5 and ERα-IRES-3) were analyzed for expression of ERα–harboring transcript (1.8 kb), Hygromycin B resistance gene-containing transcript (1.0 kb), and ERα-IRES-Hygro R fused transcript (3.2 kb), by RT followed by long range PCR amplification. pERα-IRES DNA served as a PCR positive control for the ERα cDNA primers (1.8 kb), the Hygromycin B resistance gene ORF primers (1.0 kb), and the 5′ sense ERα primer plus 3′ antisense Hygro R fused ORFs primers (3.5 kb). A First four lanes from left contain the ERα cDNA primers; lanes 5–8 the 5′ sense ERα primer together with the 3′ antisense Hygro R gene primer. B. The 5′ sense ERα primer together with the 3′ antisense Hygro R gene primer. C. Lanes 1 and 2 from left, the ERα primers. Lanes 3 and 4 the Hygro R gene primers. Primer sequences are detailed in the “ Methods ” section.

    Article Snippet: Two µg of total RNA extracted using EZ-RNA isolation kit (Biological Industries, Israel) were transcribed into first strand cDNA by hexamer priming, followed by PCR reactions as specified in the Long range RT-PCR kit (Qiagen).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Multiple Displacement Amplification, Transfection, Expressing, Polymerase Chain Reaction, Amplification, Positive Control

    RT-PCR analysis of the postulated ABC transporter genes 730–760. The positions of the different amplicons are shown below the scheme of the MHO_710–770 gene region. The primers used ( Table 1 ) and the lengths of the amplicons (A-G) are indicated. PCR products (A–G) for genomic DNA (g), mRNA (m) and cDNA (c) were separated on a 0.6% agarose gel and stained with ethidium bromide. M, Gene Ruler 1 kb DNA ladder (Fermentas).

    Journal: PLoS ONE

    Article Title: Validation of a novel Mho microarray for a comprehensive characterisation of the Mycoplasma hominis action in HeLa cell infection

    doi: 10.1371/journal.pone.0181383

    Figure Lengend Snippet: RT-PCR analysis of the postulated ABC transporter genes 730–760. The positions of the different amplicons are shown below the scheme of the MHO_710–770 gene region. The primers used ( Table 1 ) and the lengths of the amplicons (A-G) are indicated. PCR products (A–G) for genomic DNA (g), mRNA (m) and cDNA (c) were separated on a 0.6% agarose gel and stained with ethidium bromide. M, Gene Ruler 1 kb DNA ladder (Fermentas).

    Article Snippet: Overlapping regions of the MHO genes 720–770 were amplified using the Long Range PCR Kit (Qiagen, Hilden, Germany) by standard PCR conditions (initial cycle of 3 min at 93°C; 35 cycles of 15 s at 93°C, 30 s at 50°C, 10 min at 68°C).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Staining

    Mbo II digest results. Agarose gel showing Mbo II digests of GAA PCR products of FRDA samples. The expected 170bp (5′) and 120bp (3′) undigested GAA-flanking fragments from normal pure GAA repeat expansion FRDA samples are shown in lanes 2, 3, and 4. These band sizes can be seen in between the 200 and 100bp fragments of the 1 Kb+ DNA ladder markers, which are loaded into lanes 1 and 11 of the gel. Lane 5 shows a large Mbo II band of approximately 600bp that was obtained from the positive interrupted GAA repeat sequence from the “NEP” BAC transgenic mouse that contains approximately 500 triplet repeats with the previously determined interrupted sequence of (GAA) 21 (GGAGAA) 5 (GGAGGAGAA) 70 (GAA) n ( Holloway et al., 2011 ). In addition for this positive sample, we also identified the expected 5′ flanking band of 170bp, together with a smaller band of less than 100bp that we sequenced and we showed to contain a 27bp deletion in the 3′ flanking region. Lane 6 shows an abnormal band of 200bp representing the 80bp duplication in the 3′ GAA flanking region. Lane 7 shows an abnormal band of approximately 100bp representing the 19bp deletion in the 3′ GAA flanking region. Lanes 8, 9, and 10 contain abnormal bands of approximately 300, 100, and 180bp, respectively, that are likely to contain a region of interrupted GAA repeat sequence within the body of one or other of the large FRDA GAA repeat expansions.

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Large Interruptions of GAA Repeat Expansion Mutations in Friedreich Ataxia Are Very Rare

    doi: 10.3389/fncel.2018.00443

    Figure Lengend Snippet: Mbo II digest results. Agarose gel showing Mbo II digests of GAA PCR products of FRDA samples. The expected 170bp (5′) and 120bp (3′) undigested GAA-flanking fragments from normal pure GAA repeat expansion FRDA samples are shown in lanes 2, 3, and 4. These band sizes can be seen in between the 200 and 100bp fragments of the 1 Kb+ DNA ladder markers, which are loaded into lanes 1 and 11 of the gel. Lane 5 shows a large Mbo II band of approximately 600bp that was obtained from the positive interrupted GAA repeat sequence from the “NEP” BAC transgenic mouse that contains approximately 500 triplet repeats with the previously determined interrupted sequence of (GAA) 21 (GGAGAA) 5 (GGAGGAGAA) 70 (GAA) n ( Holloway et al., 2011 ). In addition for this positive sample, we also identified the expected 5′ flanking band of 170bp, together with a smaller band of less than 100bp that we sequenced and we showed to contain a 27bp deletion in the 3′ flanking region. Lane 6 shows an abnormal band of 200bp representing the 80bp duplication in the 3′ GAA flanking region. Lane 7 shows an abnormal band of approximately 100bp representing the 19bp deletion in the 3′ GAA flanking region. Lanes 8, 9, and 10 contain abnormal bands of approximately 300, 100, and 180bp, respectively, that are likely to contain a region of interrupted GAA repeat sequence within the body of one or other of the large FRDA GAA repeat expansions.

    Article Snippet: We then performed long-range PCR of the samples (approximately 100 ng input DNA) using either the Expand High Fidelity PCR System, dNTPack (Roche), or the Long Range PCR Kit (Qiagen) together with GAA-B-F (5′-AATGGATTTCCTGGCAGGACGC-3′) and GAA-B-R (5′-GCATTGGGCGATCTTGGCTTAA-3′) primers as previously described ( ).

    Techniques: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Sequencing, BAC Assay, Transgenic Assay

    Mbo II digests of GAA repeat expansions from human FRDA somatic tissues and mouse FRDA intergenerational and somatic tissues. Agarose gels showing Mbo II digests of GAA PCR products of (A) FRDA patient cerebellum tissue samples, (B) YG8sR mouse ear biopsy samples and human FRDA blood samples, and (C) four tissues from one YG8sR mouse. In each case, the expected 170 and 120bp undigested GAA-flanking fragments can be identified in between the 200 and 100bp fragments of the 1 Kb+ DNA ladder marker, which is loaded into the first lane of each gel. (A) Lanes 1–3 show the results from cerebellum tissue samples from three FRDA patients. (B) Lanes 1 and 2 are from FRDA patient blood samples; lanes 3–6 are from ear biopsy samples from 4 GAA repeat expansion-based YG8sR mice of four different generations, and lane 7 is from an ear biopsy sample from the Y47R mouse which has nine GAA repeats. (C) Lanes 1–4 are from brain, cerebellum, heart, and liver tissues of the YG8sR mouse, respectively.

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Large Interruptions of GAA Repeat Expansion Mutations in Friedreich Ataxia Are Very Rare

    doi: 10.3389/fncel.2018.00443

    Figure Lengend Snippet: Mbo II digests of GAA repeat expansions from human FRDA somatic tissues and mouse FRDA intergenerational and somatic tissues. Agarose gels showing Mbo II digests of GAA PCR products of (A) FRDA patient cerebellum tissue samples, (B) YG8sR mouse ear biopsy samples and human FRDA blood samples, and (C) four tissues from one YG8sR mouse. In each case, the expected 170 and 120bp undigested GAA-flanking fragments can be identified in between the 200 and 100bp fragments of the 1 Kb+ DNA ladder marker, which is loaded into the first lane of each gel. (A) Lanes 1–3 show the results from cerebellum tissue samples from three FRDA patients. (B) Lanes 1 and 2 are from FRDA patient blood samples; lanes 3–6 are from ear biopsy samples from 4 GAA repeat expansion-based YG8sR mice of four different generations, and lane 7 is from an ear biopsy sample from the Y47R mouse which has nine GAA repeats. (C) Lanes 1–4 are from brain, cerebellum, heart, and liver tissues of the YG8sR mouse, respectively.

    Article Snippet: We then performed long-range PCR of the samples (approximately 100 ng input DNA) using either the Expand High Fidelity PCR System, dNTPack (Roche), or the Long Range PCR Kit (Qiagen) together with GAA-B-F (5′-AATGGATTTCCTGGCAGGACGC-3′) and GAA-B-R (5′-GCATTGGGCGATCTTGGCTTAA-3′) primers as previously described ( ).

    Techniques: Polymerase Chain Reaction, Marker, Mouse Assay