long range pcr mtdna  (TaKaRa)


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    TaKaRa long range pcr mtdna
    iPSC and iPSC‐derived neurons do not exhibit <t>mtDNA</t> deletions or copy number variations. (A) Left panel: MtDNA copy number (CN) per cell measurement showing the mean with standard error of the mean calculated from six independent assays using two clones per subject. Right panel: Correlation of ND1 and ND4 Ct values in neurons showing a strong correlation (Pearson r = 0.9887) and implying that no deletions in mtDNA were present. An asterisk represents a deletion control. Gels show representative images of long‐range <t>PCR</t> analysis on DNA isolated from iPSC‐derived neurons (B) and iPSC lines (C). bp = base pair; Ct = threshold cycle; iPSC = induced pluripotent stem cell; mtDNA = mitochondrial DNA; Opa1 = optic atrophy 1.
    Long Range Pcr Mtdna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/long range pcr mtdna/product/TaKaRa
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    long range pcr mtdna - by Bioz Stars, 2021-09
    86/100 stars

    Images

    1) Product Images from "Stem cell modeling of mitochondrial parkinsonism reveals key functions of OPA1"

    Article Title: Stem cell modeling of mitochondrial parkinsonism reveals key functions of OPA1

    Journal: Annals of Neurology

    doi: 10.1002/ana.25221

    iPSC and iPSC‐derived neurons do not exhibit mtDNA deletions or copy number variations. (A) Left panel: MtDNA copy number (CN) per cell measurement showing the mean with standard error of the mean calculated from six independent assays using two clones per subject. Right panel: Correlation of ND1 and ND4 Ct values in neurons showing a strong correlation (Pearson r = 0.9887) and implying that no deletions in mtDNA were present. An asterisk represents a deletion control. Gels show representative images of long‐range PCR analysis on DNA isolated from iPSC‐derived neurons (B) and iPSC lines (C). bp = base pair; Ct = threshold cycle; iPSC = induced pluripotent stem cell; mtDNA = mitochondrial DNA; Opa1 = optic atrophy 1.
    Figure Legend Snippet: iPSC and iPSC‐derived neurons do not exhibit mtDNA deletions or copy number variations. (A) Left panel: MtDNA copy number (CN) per cell measurement showing the mean with standard error of the mean calculated from six independent assays using two clones per subject. Right panel: Correlation of ND1 and ND4 Ct values in neurons showing a strong correlation (Pearson r = 0.9887) and implying that no deletions in mtDNA were present. An asterisk represents a deletion control. Gels show representative images of long‐range PCR analysis on DNA isolated from iPSC‐derived neurons (B) and iPSC lines (C). bp = base pair; Ct = threshold cycle; iPSC = induced pluripotent stem cell; mtDNA = mitochondrial DNA; Opa1 = optic atrophy 1.

    Techniques Used: Derivative Assay, Clone Assay, Polymerase Chain Reaction, Isolation

    Related Articles

    Amplification:

    Article Title: Lack of Parkin Anticipates the Phenotype and Affects Mitochondrial Morphology and mtDNA Levels in a Mouse Model of Parkinson's Disease
    Article Snippet: .. Fifteen nanograms of DNA was amplified to generate a 10 kb fragment using a Long-Range PCR Polymerase (Takara). ..

    Article Title: Functional changes in the neural retina occur in the absence of mitochondrial dysfunction in a rodent model of diabetic retinopathy
    Article Snippet: .. mtDNA was amplified using 1 ng total DNA and long-range proofreading PCR (TaKaRa LR DNA Polymerase) with primers specific for the mitochondrial genome ( ). ..

    Article Title: Preferent Diaphragmatic Involvement in TK2 Deficiency: An Autopsy Case Study
    Article Snippet: .. DNA was examined for the presence of multiple mtDNA deletions by long-range polymerase chain-reaction (LR-PCR) through amplification of the entire 16.5 kb mtDNA using TaKaRa LA Taq DNA Polymerase (Takara-Bio, Shiga, Japan) (primers and PCR conditions available on request). ..

    Article Title: Stem cell modeling of mitochondrial parkinsonism reveals key functions of OPA1
    Article Snippet: .. mtDNA Copy Number and Deletion Levels Determination and Long‐Range PCR mtDNA was amplified in two fragments of approximately 9.9 kilobases (kb) and 15.4 kb in length using Takara PrimeSTAR GXL DNA polymerase (Takara Bio Inc., Kusatsu, Japan). ..

    Article Title: Extraocular Muscle Reveals Selective Vulnerability of Type IIB Fibers to Respiratory Chain Defects Induced by Mitochondrial DNA Alterations
    Article Snippet: .. The deleted mtDNA molecules were then amplified using TAKARA LA PCR Kit (TAKARA Bio), as described previously. ..

    Polymerase Chain Reaction:

    Article Title: Lack of Parkin Anticipates the Phenotype and Affects Mitochondrial Morphology and mtDNA Levels in a Mouse Model of Parkinson's Disease
    Article Snippet: .. Fifteen nanograms of DNA was amplified to generate a 10 kb fragment using a Long-Range PCR Polymerase (Takara). ..

    Article Title: Functional changes in the neural retina occur in the absence of mitochondrial dysfunction in a rodent model of diabetic retinopathy
    Article Snippet: .. mtDNA was amplified using 1 ng total DNA and long-range proofreading PCR (TaKaRa LR DNA Polymerase) with primers specific for the mitochondrial genome ( ). ..

    Article Title: Preferent Diaphragmatic Involvement in TK2 Deficiency: An Autopsy Case Study
    Article Snippet: .. DNA was examined for the presence of multiple mtDNA deletions by long-range polymerase chain-reaction (LR-PCR) through amplification of the entire 16.5 kb mtDNA using TaKaRa LA Taq DNA Polymerase (Takara-Bio, Shiga, Japan) (primers and PCR conditions available on request). ..

    Article Title: Stem cell modeling of mitochondrial parkinsonism reveals key functions of OPA1
    Article Snippet: .. mtDNA Copy Number and Deletion Levels Determination and Long‐Range PCR mtDNA was amplified in two fragments of approximately 9.9 kilobases (kb) and 15.4 kb in length using Takara PrimeSTAR GXL DNA polymerase (Takara Bio Inc., Kusatsu, Japan). ..

    Article Title: Extraocular Muscle Reveals Selective Vulnerability of Type IIB Fibers to Respiratory Chain Defects Induced by Mitochondrial DNA Alterations
    Article Snippet: .. The deleted mtDNA molecules were then amplified using TAKARA LA PCR Kit (TAKARA Bio), as described previously. ..

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    TaKaRa long range pcr mtdna
    iPSC and iPSC‐derived neurons do not exhibit <t>mtDNA</t> deletions or copy number variations. (A) Left panel: MtDNA copy number (CN) per cell measurement showing the mean with standard error of the mean calculated from six independent assays using two clones per subject. Right panel: Correlation of ND1 and ND4 Ct values in neurons showing a strong correlation (Pearson r = 0.9887) and implying that no deletions in mtDNA were present. An asterisk represents a deletion control. Gels show representative images of long‐range <t>PCR</t> analysis on DNA isolated from iPSC‐derived neurons (B) and iPSC lines (C). bp = base pair; Ct = threshold cycle; iPSC = induced pluripotent stem cell; mtDNA = mitochondrial DNA; Opa1 = optic atrophy 1.
    Long Range Pcr Mtdna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/long range pcr mtdna/product/TaKaRa
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    long range pcr mtdna - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

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    TaKaRa mtdna deletions long range pcr
    iPSC and iPSC‐derived neurons do not exhibit <t>mtDNA</t> deletions or copy number variations. (A) Left panel: MtDNA copy number (CN) per cell measurement showing the mean with standard error of the mean calculated from six independent assays using two clones per subject. Right panel: Correlation of ND1 and ND4 Ct values in neurons showing a strong correlation (Pearson r = 0.9887) and implying that no deletions in mtDNA were present. An asterisk represents a deletion control. Gels show representative images of long‐range <t>PCR</t> analysis on DNA isolated from iPSC‐derived neurons (B) and iPSC lines (C). bp = base pair; Ct = threshold cycle; iPSC = induced pluripotent stem cell; mtDNA = mitochondrial DNA; Opa1 = optic atrophy 1.
    Mtdna Deletions Long Range Pcr, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mtdna deletions long range pcr/product/TaKaRa
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mtdna deletions long range pcr - by Bioz Stars, 2021-09
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    TaKaRa la taq polymerase mix
    iPSC and iPSC‐derived neurons do not exhibit <t>mtDNA</t> deletions or copy number variations. (A) Left panel: MtDNA copy number (CN) per cell measurement showing the mean with standard error of the mean calculated from six independent assays using two clones per subject. Right panel: Correlation of ND1 and ND4 Ct values in neurons showing a strong correlation (Pearson r = 0.9887) and implying that no deletions in mtDNA were present. An asterisk represents a deletion control. Gels show representative images of long‐range <t>PCR</t> analysis on DNA isolated from iPSC‐derived neurons (B) and iPSC lines (C). bp = base pair; Ct = threshold cycle; iPSC = induced pluripotent stem cell; mtDNA = mitochondrial DNA; Opa1 = optic atrophy 1.
    La Taq Polymerase Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa primestar gxl dna polymerase qiaqen
    iPSC and iPSC‐derived neurons do not exhibit <t>mtDNA</t> deletions or copy number variations. (A) Left panel: MtDNA copy number (CN) per cell measurement showing the mean with standard error of the mean calculated from six independent assays using two clones per subject. Right panel: Correlation of ND1 and ND4 Ct values in neurons showing a strong correlation (Pearson r = 0.9887) and implying that no deletions in mtDNA were present. An asterisk represents a deletion control. Gels show representative images of long‐range <t>PCR</t> analysis on DNA isolated from iPSC‐derived neurons (B) and iPSC lines (C). bp = base pair; Ct = threshold cycle; iPSC = induced pluripotent stem cell; mtDNA = mitochondrial DNA; Opa1 = optic atrophy 1.
    Primestar Gxl Dna Polymerase Qiaqen, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primestar gxl dna polymerase qiaqen/product/TaKaRa
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    Image Search Results


    iPSC and iPSC‐derived neurons do not exhibit mtDNA deletions or copy number variations. (A) Left panel: MtDNA copy number (CN) per cell measurement showing the mean with standard error of the mean calculated from six independent assays using two clones per subject. Right panel: Correlation of ND1 and ND4 Ct values in neurons showing a strong correlation (Pearson r = 0.9887) and implying that no deletions in mtDNA were present. An asterisk represents a deletion control. Gels show representative images of long‐range PCR analysis on DNA isolated from iPSC‐derived neurons (B) and iPSC lines (C). bp = base pair; Ct = threshold cycle; iPSC = induced pluripotent stem cell; mtDNA = mitochondrial DNA; Opa1 = optic atrophy 1.

    Journal: Annals of Neurology

    Article Title: Stem cell modeling of mitochondrial parkinsonism reveals key functions of OPA1

    doi: 10.1002/ana.25221

    Figure Lengend Snippet: iPSC and iPSC‐derived neurons do not exhibit mtDNA deletions or copy number variations. (A) Left panel: MtDNA copy number (CN) per cell measurement showing the mean with standard error of the mean calculated from six independent assays using two clones per subject. Right panel: Correlation of ND1 and ND4 Ct values in neurons showing a strong correlation (Pearson r = 0.9887) and implying that no deletions in mtDNA were present. An asterisk represents a deletion control. Gels show representative images of long‐range PCR analysis on DNA isolated from iPSC‐derived neurons (B) and iPSC lines (C). bp = base pair; Ct = threshold cycle; iPSC = induced pluripotent stem cell; mtDNA = mitochondrial DNA; Opa1 = optic atrophy 1.

    Article Snippet: mtDNA Copy Number and Deletion Levels Determination and Long‐Range PCR mtDNA was amplified in two fragments of approximately 9.9 kilobases (kb) and 15.4 kb in length using Takara PrimeSTAR GXL DNA polymerase (Takara Bio Inc., Kusatsu, Japan).

    Techniques: Derivative Assay, Clone Assay, Polymerase Chain Reaction, Isolation