long range dntpack kit  (Roche)


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    Structured Review

    Roche long range dntpack kit
    Long Range Dntpack Kit, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/long range dntpack kit/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    long range dntpack kit - by Bioz Stars, 2021-06
    86/100 stars

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    Related Articles

    Sequencing:

    Article Title: Mitochondrial genome evolution in species belonging to the Phialocephala fortinii s.l. - Acephala applanata species complex
    Article Snippet: Sequencing the complete mt genome of Phialocephala subalpina In the course of a genome sequencing project of P. subalpina strain UAMH 11012, an initial Roche/454 GS FLX (454) shotgun run was performed at the Functional Genomics Centre Zurich (FGCZ, Uni/ETH Zurich) and from that a draft of the circular mt genome of P. subalpina strain UAMH 11012 became available. .. The draft sequence was subdivided into 12 fragments (see Additional file ) and amplified from strain UAMH 11012 using long-range PCR in 20 μl volumes (Expand Long Range dNTPack kit, Roche, Rotkreuz, Switzerland). .. PCR conditions were optimized for 4 kb fragments with high AT contents by lowering the temperature during the elongation step from 68°C to 62°C and an initial elongation time of 4 min. PCR fragments were purified using the Wizard Plus SV kit (Promega, Wallisellen, Switzerland) and sequenced at Microsynth (Balgach, Switzerland).

    Amplification:

    Article Title: Mitochondrial genome evolution in species belonging to the Phialocephala fortinii s.l. - Acephala applanata species complex
    Article Snippet: Sequencing the complete mt genome of Phialocephala subalpina In the course of a genome sequencing project of P. subalpina strain UAMH 11012, an initial Roche/454 GS FLX (454) shotgun run was performed at the Functional Genomics Centre Zurich (FGCZ, Uni/ETH Zurich) and from that a draft of the circular mt genome of P. subalpina strain UAMH 11012 became available. .. The draft sequence was subdivided into 12 fragments (see Additional file ) and amplified from strain UAMH 11012 using long-range PCR in 20 μl volumes (Expand Long Range dNTPack kit, Roche, Rotkreuz, Switzerland). .. PCR conditions were optimized for 4 kb fragments with high AT contents by lowering the temperature during the elongation step from 68°C to 62°C and an initial elongation time of 4 min. PCR fragments were purified using the Wizard Plus SV kit (Promega, Wallisellen, Switzerland) and sequenced at Microsynth (Balgach, Switzerland).

    Article Title: Conserved Catalytic and C-Terminal Regulatory Domains of the C-Terminal Binding Protein Corepressor Fine-Tune the Transcriptional Response in Development ▿
    Article Snippet: The mutant phenotypes indicate that the putative dehydrogenase function, as well as the C-terminal regulatory domain, are in fact essential for the normal developmental activity of this corepressor. .. The 16-kb annotated Drosophila CtBP gene region was divided into three pieces, named CtBP- 1st, CtBP- 2nd, and CtBP- 3rd, and amplified from yw adult genomic DNA using an expand long range dNTPack kit (Roche). ..

    Polymerase Chain Reaction:

    Article Title: Mitochondrial genome evolution in species belonging to the Phialocephala fortinii s.l. - Acephala applanata species complex
    Article Snippet: Sequencing the complete mt genome of Phialocephala subalpina In the course of a genome sequencing project of P. subalpina strain UAMH 11012, an initial Roche/454 GS FLX (454) shotgun run was performed at the Functional Genomics Centre Zurich (FGCZ, Uni/ETH Zurich) and from that a draft of the circular mt genome of P. subalpina strain UAMH 11012 became available. .. The draft sequence was subdivided into 12 fragments (see Additional file ) and amplified from strain UAMH 11012 using long-range PCR in 20 μl volumes (Expand Long Range dNTPack kit, Roche, Rotkreuz, Switzerland). .. PCR conditions were optimized for 4 kb fragments with high AT contents by lowering the temperature during the elongation step from 68°C to 62°C and an initial elongation time of 4 min. PCR fragments were purified using the Wizard Plus SV kit (Promega, Wallisellen, Switzerland) and sequenced at Microsynth (Balgach, Switzerland).

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    Roche expand long template pcr system kit
    Agar gel electrophoretic analysis of the <t>PCR</t> <t>POLH</t> gDNA of exon 10 and its intronic boundaries showed difference in the size between affected individuals (XPV17B-1 and XPV91) compared to healthy parents (XPV(P)) and a healthy control. (Marker: 1 kb DNA ladder molecular size marker (GeneRuler).)
    Expand Long Template Pcr System Kit, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expand long template pcr system kit/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    expand long template pcr system kit - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    86
    Roche expand long range dntpack kit
    Agar gel electrophoretic analysis of the <t>PCR</t> <t>POLH</t> gDNA of exon 10 and its intronic boundaries showed difference in the size between affected individuals (XPV17B-1 and XPV91) compared to healthy parents (XPV(P)) and a healthy control. (Marker: 1 kb DNA ladder molecular size marker (GeneRuler).)
    Expand Long Range Dntpack Kit, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expand long range dntpack kit/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    expand long range dntpack kit - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    Image Search Results


    Agar gel electrophoretic analysis of the PCR POLH gDNA of exon 10 and its intronic boundaries showed difference in the size between affected individuals (XPV17B-1 and XPV91) compared to healthy parents (XPV(P)) and a healthy control. (Marker: 1 kb DNA ladder molecular size marker (GeneRuler).)

    Journal: BioMed Research International

    Article Title: A Founder Large Deletion Mutation in Xeroderma Pigmentosum-Variant Form in Tunisia: Implication for Molecular Diagnosis and Therapy

    doi: 10.1155/2014/256245

    Figure Lengend Snippet: Agar gel electrophoretic analysis of the PCR POLH gDNA of exon 10 and its intronic boundaries showed difference in the size between affected individuals (XPV17B-1 and XPV91) compared to healthy parents (XPV(P)) and a healthy control. (Marker: 1 kb DNA ladder molecular size marker (GeneRuler).)

    Article Snippet: PCR Long-Range On absence of amplification of POLH exon 10, long PCR was performed using the Expand Long Template PCR System Kit (Expand Long Range dNTPack 700 units/μ L Roche).

    Techniques: Polymerase Chain Reaction, Marker