long fragment polymerase chain reaction pcr  (Roche)

 
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    Structured Review

    Roche long fragment polymerase chain reaction pcr
    Long Fragment Polymerase Chain Reaction Pcr, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/long fragment polymerase chain reaction pcr/product/Roche
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    long fragment polymerase chain reaction pcr - by Bioz Stars, 2020-07
    85/100 stars

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    expanded long template pcr system kit

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    Polymerase Chain Reaction:

    Article Title: A mouse model of sitosterolemia: absence of Abcg8/sterolin-2 results in failure to secrete biliary cholesterol
    Article Snippet: .. Long-fragment polymerase chain reaction (PCR) was performed using Expanded Long Template PCR system kit (Roche Applied Science, Indianapolis, IA, USA). .. An approximately 4.5 kb 'long-arm' genomic fragment containing partial exon 1 to partial exon 3 was inserted into the Pml I restriction-cloning site A of OSDUPDEL vector.

    Article Title: Expression and function of Abcg4 in the mouse blood-brain barrier: role in restricting the brain entry of amyloid-β peptide
    Article Snippet: .. Long-fragment polymerase chain reaction (PCR) was performed using Expanded Long Template PCR system kit (Roche Applied Science, Indianapolis, IA, USA). .. An approximately 1.7 kb ‘long-arm’ genomic fragment containing exon 6 to partial exon 7 was inserted in-frame into the coding sequence for enhanced green fluorescent protein (pCRII, Invitrogen) and the exon 6–exon 7-eGFP fusion fragment (5’ arm, ‘short-arm’) was cloned into cloning site A of OSDUPDEL vector (supplied by Dr. Nobuyo Maeda, University of North Carolina) and verified by sequencing.

    IA:

    Article Title: A mouse model of sitosterolemia: absence of Abcg8/sterolin-2 results in failure to secrete biliary cholesterol
    Article Snippet: .. Long-fragment polymerase chain reaction (PCR) was performed using Expanded Long Template PCR system kit (Roche Applied Science, Indianapolis, IA, USA). .. An approximately 4.5 kb 'long-arm' genomic fragment containing partial exon 1 to partial exon 3 was inserted into the Pml I restriction-cloning site A of OSDUPDEL vector.

    Article Title: Expression and function of Abcg4 in the mouse blood-brain barrier: role in restricting the brain entry of amyloid-β peptide
    Article Snippet: .. Long-fragment polymerase chain reaction (PCR) was performed using Expanded Long Template PCR system kit (Roche Applied Science, Indianapolis, IA, USA). .. An approximately 1.7 kb ‘long-arm’ genomic fragment containing exon 6 to partial exon 7 was inserted in-frame into the coding sequence for enhanced green fluorescent protein (pCRII, Invitrogen) and the exon 6–exon 7-eGFP fusion fragment (5’ arm, ‘short-arm’) was cloned into cloning site A of OSDUPDEL vector (supplied by Dr. Nobuyo Maeda, University of North Carolina) and verified by sequencing.

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  • 88
    Roche long range pcr fragments
    Screening for mtDNA-deletions in <t>DNA</t> extracts from muscle and blood of the patient. ( a ) Two overlapping long-range <t>PCR</t> fragments were generated that span the entire mtDNA. The shorter elongation time of 4 min amplifies preferentially the shorter fragments as seen in the samples of two patients with Kearns-Sayre Syndrome (KSS). ( b ) Position of the primers and the mtDNA coverage of the long-range PCR products. ( c ) Southern blot autoradiography of Pvu II digested DNA labeled with a radioactive mtDNA probe. *Other diagnostic samples not related to the study
    Long Range Pcr Fragments, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/long range pcr fragments/product/Roche
    Average 88 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    long range pcr fragments - by Bioz Stars, 2020-07
    88/100 stars
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    88
    Roche orf3 gene
    (A) Conserved sequences in the intergenic regions upstream of ORF2, <t>ORF3</t> and ORF4 in the reference genotype 1 (THA-98-Ref) and genotype 2 (AUS-96-Ref) isolates and representatives of genotypes 3 (VNM-02-H93), 4 (IND-02-H9) and 5 (THA-03-SG21). The initiation and termination codons of flanking ORFs are indicated in bold face and underlined. The position in each sequence corresponding to the 5′-terminal adenosine of GAV sgmRNA1 and sgmRNA2 is shaded. The highly conserved 14 nt sequence surrounding the terminal adenosine is underlined, as is the region upstream of ORF4 in which the sequence is partially conserved. Nucleotide substitutions (uridine) in the conserved regions are shaded in black. Identical nucleotides in all aligned sequences are indicated (⁎). (B) WebLogo presentation of nucleotides conserved and less conserved between genotypes 1, 2, 3, 4 and 5 in the TRS elements in IGR1 and in IGR2 as well as between the TRS elements of both IGRs. The cDNA rather than RNA sequence was analysed. (C) Multiple alignment of the putative genotype 2 (GAV) ORF4 gene amino acid sequence with the comparable truncated sequences of genotypes 1, 3, 4 and 5. Amino acids shared in all 5 genotypes are indicated (⁎).
    Orf3 Gene, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/orf3 gene/product/Roche
    Average 88 stars, based on 2 article reviews
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    88
    Roche long range pcr amplified fragments
    (A) Conserved sequences in the intergenic regions upstream of ORF2, <t>ORF3</t> and ORF4 in the reference genotype 1 (THA-98-Ref) and genotype 2 (AUS-96-Ref) isolates and representatives of genotypes 3 (VNM-02-H93), 4 (IND-02-H9) and 5 (THA-03-SG21). The initiation and termination codons of flanking ORFs are indicated in bold face and underlined. The position in each sequence corresponding to the 5′-terminal adenosine of GAV sgmRNA1 and sgmRNA2 is shaded. The highly conserved 14 nt sequence surrounding the terminal adenosine is underlined, as is the region upstream of ORF4 in which the sequence is partially conserved. Nucleotide substitutions (uridine) in the conserved regions are shaded in black. Identical nucleotides in all aligned sequences are indicated (⁎). (B) WebLogo presentation of nucleotides conserved and less conserved between genotypes 1, 2, 3, 4 and 5 in the TRS elements in IGR1 and in IGR2 as well as between the TRS elements of both IGRs. The cDNA rather than RNA sequence was analysed. (C) Multiple alignment of the putative genotype 2 (GAV) ORF4 gene amino acid sequence with the comparable truncated sequences of genotypes 1, 3, 4 and 5. Amino acids shared in all 5 genotypes are indicated (⁎).
    Long Range Pcr Amplified Fragments, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/long range pcr amplified fragments/product/Roche
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    long range pcr amplified fragments - by Bioz Stars, 2020-07
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    Screening for mtDNA-deletions in DNA extracts from muscle and blood of the patient. ( a ) Two overlapping long-range PCR fragments were generated that span the entire mtDNA. The shorter elongation time of 4 min amplifies preferentially the shorter fragments as seen in the samples of two patients with Kearns-Sayre Syndrome (KSS). ( b ) Position of the primers and the mtDNA coverage of the long-range PCR products. ( c ) Southern blot autoradiography of Pvu II digested DNA labeled with a radioactive mtDNA probe. *Other diagnostic samples not related to the study

    Journal: JIMD Reports

    Article Title: Muscle Weakness, Cardiomyopathy, and L-2-Hydroxyglutaric Aciduria Associated with a Novel Recessive SLC25A4 Mutation

    doi: 10.1007/8904_2018_93

    Figure Lengend Snippet: Screening for mtDNA-deletions in DNA extracts from muscle and blood of the patient. ( a ) Two overlapping long-range PCR fragments were generated that span the entire mtDNA. The shorter elongation time of 4 min amplifies preferentially the shorter fragments as seen in the samples of two patients with Kearns-Sayre Syndrome (KSS). ( b ) Position of the primers and the mtDNA coverage of the long-range PCR products. ( c ) Southern blot autoradiography of Pvu II digested DNA labeled with a radioactive mtDNA probe. *Other diagnostic samples not related to the study

    Article Snippet: For long-range PCR, two overlapping long-range PCR fragments were generated from whole DNA extracts of muscle and blood using a proof-reading long-range polymerase (Roche) and two oligonucleotide primer sets FOR#1: 5′-CCCTCTCTCCTACTCCTG-3′, REV#1: 5′-CAGGTGGTCAAGTATTTATGG-3′ (PCR-product size 9,932 bp), and FOR#2: 5′-CTTTATCTGCCTCTTCCTACACATCG-3′, REV#2: 5′-GTATGTAGGAGTTGAAGATTAGTCCGCC-3′ (PCR-product size 9,506 bp), which were separated on an 0.8% agarose gel along with control samples and a high molecular size standard.

    Techniques: Polymerase Chain Reaction, Generated, Southern Blot, Autoradiography, Labeling, Diagnostic Assay

    (A) Conserved sequences in the intergenic regions upstream of ORF2, ORF3 and ORF4 in the reference genotype 1 (THA-98-Ref) and genotype 2 (AUS-96-Ref) isolates and representatives of genotypes 3 (VNM-02-H93), 4 (IND-02-H9) and 5 (THA-03-SG21). The initiation and termination codons of flanking ORFs are indicated in bold face and underlined. The position in each sequence corresponding to the 5′-terminal adenosine of GAV sgmRNA1 and sgmRNA2 is shaded. The highly conserved 14 nt sequence surrounding the terminal adenosine is underlined, as is the region upstream of ORF4 in which the sequence is partially conserved. Nucleotide substitutions (uridine) in the conserved regions are shaded in black. Identical nucleotides in all aligned sequences are indicated (⁎). (B) WebLogo presentation of nucleotides conserved and less conserved between genotypes 1, 2, 3, 4 and 5 in the TRS elements in IGR1 and in IGR2 as well as between the TRS elements of both IGRs. The cDNA rather than RNA sequence was analysed. (C) Multiple alignment of the putative genotype 2 (GAV) ORF4 gene amino acid sequence with the comparable truncated sequences of genotypes 1, 3, 4 and 5. Amino acids shared in all 5 genotypes are indicated (⁎).

    Journal: Virology

    Article Title: Genetic diversity in the yellow head nidovirus complex

    doi: 10.1016/j.virol.2008.07.005

    Figure Lengend Snippet: (A) Conserved sequences in the intergenic regions upstream of ORF2, ORF3 and ORF4 in the reference genotype 1 (THA-98-Ref) and genotype 2 (AUS-96-Ref) isolates and representatives of genotypes 3 (VNM-02-H93), 4 (IND-02-H9) and 5 (THA-03-SG21). The initiation and termination codons of flanking ORFs are indicated in bold face and underlined. The position in each sequence corresponding to the 5′-terminal adenosine of GAV sgmRNA1 and sgmRNA2 is shaded. The highly conserved 14 nt sequence surrounding the terminal adenosine is underlined, as is the region upstream of ORF4 in which the sequence is partially conserved. Nucleotide substitutions (uridine) in the conserved regions are shaded in black. Identical nucleotides in all aligned sequences are indicated (⁎). (B) WebLogo presentation of nucleotides conserved and less conserved between genotypes 1, 2, 3, 4 and 5 in the TRS elements in IGR1 and in IGR2 as well as between the TRS elements of both IGRs. The cDNA rather than RNA sequence was analysed. (C) Multiple alignment of the putative genotype 2 (GAV) ORF4 gene amino acid sequence with the comparable truncated sequences of genotypes 1, 3, 4 and 5. Amino acids shared in all 5 genotypes are indicated (⁎).

    Article Snippet: These and subsequent PCRs to generate long fragments of the ORF3 gene employed the Expand Long Template PCR system (Roche) with Buffer 3 adjusted to contain 3.0 mM MgCl2 according to the manufacturer's instructions.

    Techniques: Sequencing

    A ClustalX multiple alignment of amino acid sequences spanning the N-terminal gp116 region of the ORF3 gene of the reference genotype 1 (THA-98-Ref) and genotype 2 (AUS-96-Ref) isolates, and representatives of genotypes 3 (VNM-02-H93), 4 (IND-02-H9) and 5 (THA-03-SG21). The site of proteolytic cleavage of the YHV pp3 polyprotein is indicated. Absolutely conserved (⁎) and similar (: or .) amino acids are indicated according to the similarity groups defined in ClustalX. Conserved cysteine and residues are indicated (+). Potential N-linked glycosylation sites are indicated in bold face and underlined in the alignment and denoted ( ) on the illustration. Six predicted transmembrane-spanning domains (TM1–TM6) are numbered.

    Journal: Virology

    Article Title: Genetic diversity in the yellow head nidovirus complex

    doi: 10.1016/j.virol.2008.07.005

    Figure Lengend Snippet: A ClustalX multiple alignment of amino acid sequences spanning the N-terminal gp116 region of the ORF3 gene of the reference genotype 1 (THA-98-Ref) and genotype 2 (AUS-96-Ref) isolates, and representatives of genotypes 3 (VNM-02-H93), 4 (IND-02-H9) and 5 (THA-03-SG21). The site of proteolytic cleavage of the YHV pp3 polyprotein is indicated. Absolutely conserved (⁎) and similar (: or .) amino acids are indicated according to the similarity groups defined in ClustalX. Conserved cysteine and residues are indicated (+). Potential N-linked glycosylation sites are indicated in bold face and underlined in the alignment and denoted ( ) on the illustration. Six predicted transmembrane-spanning domains (TM1–TM6) are numbered.

    Article Snippet: These and subsequent PCRs to generate long fragments of the ORF3 gene employed the Expand Long Template PCR system (Roche) with Buffer 3 adjusted to contain 3.0 mM MgCl2 according to the manufacturer's instructions.

    Techniques: