Structured Review

Elicityl lnno
CLL subset 4 IGs do not bind i or I carbohydrate antigens. (A) Glycan sequences of the lipid-linked oligosaccharides <t>pLNH,</t> pLNnH, <t>LNnO</t> and I-octa that were among 76 additional (i- and Fc10.2- antigen-negative probes) in the microarray. Abbreviations: Gal, galactose; GlcNAc, N-acetylglucosamine; Glc, glucose; DH, L-1,2-dihexadecyl- sn -glycero-3-phosphoethanolamine; Cer, ceramide. (B), (C) Microarray analysis of CLL mAbs 141, 282, 183, 240, and 342, in either (B) native bivalent or (C) precomplexed condition. The human anti-i IgM PIA ELL, human anti-I Ma, and the murine IgM Fc10.2 were included as positive controls. The results are the means of fluorescence intensities of duplicate spots, printed at 5 fmol, with error bars representing half the difference between the two values.
Lnno, supplied by Elicityl, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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1) Product Images from "Binding of CLL Subset 4 B Cell Receptor Immunoglobulins to Viable Human Memory B Lymphocytes Requires a Distinctive IGKV Somatic Mutation"

Article Title: Binding of CLL Subset 4 B Cell Receptor Immunoglobulins to Viable Human Memory B Lymphocytes Requires a Distinctive IGKV Somatic Mutation

Journal: Molecular Medicine

doi: 10.2119/molmed.2017.00003

CLL subset 4 IGs do not bind i or I carbohydrate antigens. (A) Glycan sequences of the lipid-linked oligosaccharides pLNH, pLNnH, LNnO and I-octa that were among 76 additional (i- and Fc10.2- antigen-negative probes) in the microarray. Abbreviations: Gal, galactose; GlcNAc, N-acetylglucosamine; Glc, glucose; DH, L-1,2-dihexadecyl- sn -glycero-3-phosphoethanolamine; Cer, ceramide. (B), (C) Microarray analysis of CLL mAbs 141, 282, 183, 240, and 342, in either (B) native bivalent or (C) precomplexed condition. The human anti-i IgM PIA ELL, human anti-I Ma, and the murine IgM Fc10.2 were included as positive controls. The results are the means of fluorescence intensities of duplicate spots, printed at 5 fmol, with error bars representing half the difference between the two values.
Figure Legend Snippet: CLL subset 4 IGs do not bind i or I carbohydrate antigens. (A) Glycan sequences of the lipid-linked oligosaccharides pLNH, pLNnH, LNnO and I-octa that were among 76 additional (i- and Fc10.2- antigen-negative probes) in the microarray. Abbreviations: Gal, galactose; GlcNAc, N-acetylglucosamine; Glc, glucose; DH, L-1,2-dihexadecyl- sn -glycero-3-phosphoethanolamine; Cer, ceramide. (B), (C) Microarray analysis of CLL mAbs 141, 282, 183, 240, and 342, in either (B) native bivalent or (C) precomplexed condition. The human anti-i IgM PIA ELL, human anti-I Ma, and the murine IgM Fc10.2 were included as positive controls. The results are the means of fluorescence intensities of duplicate spots, printed at 5 fmol, with error bars representing half the difference between the two values.

Techniques Used: Microarray, Gas Chromatography, Fluorescence

Related Articles

Conjugation Assay:

Article Title: Binding of CLL Subset 4 B Cell Receptor Immunoglobulins to Viable Human Memory B Lymphocytes Requires a Distinctive IGKV Somatic Mutation
Article Snippet: .. Two hexasaccharides, pLNH and pLNnH, from Dextra and an octasaccharide, LNnO, from Elicityl were analyzed as lipid-linked glycan probes, neoglycolipids (NGLs) derived by conjugation to a phospholipid L-1,2-dihexadecyl- sn -glycero-3-phosphoethanolamine (DHPE) ( ). .. A ceramide-linked octasaccharide I-octa was isolated from human red cells (gift of Peter Hanfland).

Derivative Assay:

Article Title: Binding of CLL Subset 4 B Cell Receptor Immunoglobulins to Viable Human Memory B Lymphocytes Requires a Distinctive IGKV Somatic Mutation
Article Snippet: .. Two hexasaccharides, pLNH and pLNnH, from Dextra and an octasaccharide, LNnO, from Elicityl were analyzed as lipid-linked glycan probes, neoglycolipids (NGLs) derived by conjugation to a phospholipid L-1,2-dihexadecyl- sn -glycero-3-phosphoethanolamine (DHPE) ( ). .. A ceramide-linked octasaccharide I-octa was isolated from human red cells (gift of Peter Hanfland).

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    Elicityl lnno
    CLL subset 4 IGs do not bind i or I carbohydrate antigens. (A) Glycan sequences of the lipid-linked oligosaccharides <t>pLNH,</t> pLNnH, <t>LNnO</t> and I-octa that were among 76 additional (i- and Fc10.2- antigen-negative probes) in the microarray. Abbreviations: Gal, galactose; GlcNAc, N-acetylglucosamine; Glc, glucose; DH, L-1,2-dihexadecyl- sn -glycero-3-phosphoethanolamine; Cer, ceramide. (B), (C) Microarray analysis of CLL mAbs 141, 282, 183, 240, and 342, in either (B) native bivalent or (C) precomplexed condition. The human anti-i IgM PIA ELL, human anti-I Ma, and the murine IgM Fc10.2 were included as positive controls. The results are the means of fluorescence intensities of duplicate spots, printed at 5 fmol, with error bars representing half the difference between the two values.
    Lnno, supplied by Elicityl, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lnno/product/Elicityl
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lnno - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

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    CLL subset 4 IGs do not bind i or I carbohydrate antigens. (A) Glycan sequences of the lipid-linked oligosaccharides pLNH, pLNnH, LNnO and I-octa that were among 76 additional (i- and Fc10.2- antigen-negative probes) in the microarray. Abbreviations: Gal, galactose; GlcNAc, N-acetylglucosamine; Glc, glucose; DH, L-1,2-dihexadecyl- sn -glycero-3-phosphoethanolamine; Cer, ceramide. (B), (C) Microarray analysis of CLL mAbs 141, 282, 183, 240, and 342, in either (B) native bivalent or (C) precomplexed condition. The human anti-i IgM PIA ELL, human anti-I Ma, and the murine IgM Fc10.2 were included as positive controls. The results are the means of fluorescence intensities of duplicate spots, printed at 5 fmol, with error bars representing half the difference between the two values.

    Journal: Molecular Medicine

    Article Title: Binding of CLL Subset 4 B Cell Receptor Immunoglobulins to Viable Human Memory B Lymphocytes Requires a Distinctive IGKV Somatic Mutation

    doi: 10.2119/molmed.2017.00003

    Figure Lengend Snippet: CLL subset 4 IGs do not bind i or I carbohydrate antigens. (A) Glycan sequences of the lipid-linked oligosaccharides pLNH, pLNnH, LNnO and I-octa that were among 76 additional (i- and Fc10.2- antigen-negative probes) in the microarray. Abbreviations: Gal, galactose; GlcNAc, N-acetylglucosamine; Glc, glucose; DH, L-1,2-dihexadecyl- sn -glycero-3-phosphoethanolamine; Cer, ceramide. (B), (C) Microarray analysis of CLL mAbs 141, 282, 183, 240, and 342, in either (B) native bivalent or (C) precomplexed condition. The human anti-i IgM PIA ELL, human anti-I Ma, and the murine IgM Fc10.2 were included as positive controls. The results are the means of fluorescence intensities of duplicate spots, printed at 5 fmol, with error bars representing half the difference between the two values.

    Article Snippet: Two hexasaccharides, pLNH and pLNnH, from Dextra and an octasaccharide, LNnO, from Elicityl were analyzed as lipid-linked glycan probes, neoglycolipids (NGLs) derived by conjugation to a phospholipid L-1,2-dihexadecyl- sn -glycero-3-phosphoethanolamine (DHPE) ( ).

    Techniques: Microarray, Gas Chromatography, Fluorescence