lncap prostate cancer cells  (ATCC)

Journal: PLoS ONE

Article Title: Curcumin Attenuates β-catenin Signaling in Prostate Cancer Cells through Activation of Protein Kinase D1

doi: 10.1371/journal.pone.0035368

Figure Lengend Snippet: A). Chemical structure of curcumin. B). Effect of curcumin on proliferation of various prostate cancer cell lines. LNCaP, C4-2, DU145 and PC3 cell were treated with curcumin or vehicle control DMSO for 48 h and cell proliferation was determined using MTS assay. The percent cell proliferation was calculated by normalizing the proliferation of curcumin treated cells with proliferation of control treated cells. Concentration dependent inhibition in cell proliferation was observed with curcumin treatment. Mean ± SE; n = 3; *p<0.05.

Article Snippet: C4-2 (UroCor, Oklahoma City, OK), PC3, and LNCaP prostate cancer cells (ATCC, Manassas, Virginia) and C4-2 cells overexpressing PKD1 were maintained in RPMI 1640 media containing 1 mM sodium pyruvate, 2.5 mM glutamine, 10% heat-inactivate FBS (Hyclone) and 1× antibiotic and antimycotic solution.

Techniques: MTS Assay, Concentration Assay, Inhibition

Journal: International Journal of Endocrinology

Article Title: Sex Hormone Binding Globulin Modifies Testosterone Action and Metabolism in Prostate Cancer Cells

doi: 10.1155/2016/6437585

Figure Lengend Snippet: Effects of testosterone (Te) and Sex Hormone Binding Globulin (SHBG) on prostate specific antigen (PSA), androgen receptor (AR), and SHBG mRNA expression in LNCaP cells by quantitative Real-Time PCR. (a) PSA mRNA expression in cells treated with 0, 1, 10, and 25 nM Te ± 25 nM SHBG for 24 hours. (b) PSA mRNA expression in cells treated with 25 nM SHBG plus 0, 1, 5, 10, or 25 nM Te. Expression of PSA (c), AR (d), and SHBG (e) mRNA in LNCaP cells treated with 10 nM Te plus 0, 10, 25, or 50 nM SHBG for 24 hours. All experiments were performed at least three times in triplicate. The mRNA expression was normalised to the housekeeping gene, β 2-microglobulin (B2M). One-way ANOVA with Tukey's test was used for statistical analysis. Tukey's test indicated as “no significance” (ns) or asterisk ( ∗ P < 0.05; ∗∗∗ P < 0.001; and ∗∗∗∗ P < 0.0001). All results were represented as mean ± standard error (SEM).

Article Snippet: The androgen responsive human prostate cancer cell line LNCaP clone FGC (ATCC, VA, USA) was chosen for this study.

Techniques: Binding Assay, Expressing, Real-time Polymerase Chain Reaction

Journal: Molecular Cancer

Article Title: Pharmacologic disruption of Polycomb Repressive Complex 2 inhibits tumorigenicity and tumor progression in prostate cancer

doi: 10.1186/1476-4598-10-40

Figure Lengend Snippet: Effects of DZNeP on invasion and in vivo tumor growth . A, Cells were treated with DZNeP (1 μM, 3 days) and assayed for cell viability at the end of the treatment (trypan blue staining). Alive cells were used for Matrigel invasion assay, as described in "Materials and Methods". Columns, mean volume; bars, standard deviation. **p < 0.01 with respect to untreated cells (T test). B, mRNA levels in invading and non-invading cells (QT-PCR). C, effects of DZNeP on LNCaP cell tumorigenicity. **p < 0.01 (log rank test) with respect to untreated cells. D, effects of DZNeP on DU145 xenograft tumor volume. *p < 0.05 with respect to untreated cells (U test). Star colors refer to dose treatment (blue, 1 μM; red, 10 μM). Number of animals: untreated: 6; 1 and 10 μM: 8. Point, mean value; bar, standard deviation.

Article Snippet: The PC cell lines LNCaP and DU145 were obtained from American Type Culture Collection (Manassas, VA).

Techniques: In Vivo, Staining, Invasion Assay, Standard Deviation

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: miR-143 Induces the Apoptosis of Prostate Cancer LNCap Cells by Suppressing Bcl-2 Expression

doi: 10.12659/MSM.899719

Figure Lengend Snippet: Western blot analyses of Bcl-2 expression in LNCap cells in the miR-143 and miRNA groups.

Article Snippet: The prostate cancer LNCap cells were purchased from American Type Culture Collection (ATCC, Rockville, Maryland, USA).

Techniques: Western Blot, Expressing

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: miR-143 Induces the Apoptosis of Prostate Cancer LNCap Cells by Suppressing Bcl-2 Expression

doi: 10.12659/MSM.899719

Figure Lengend Snippet: Bcl-2 interference enhanced the miR-143-induced apoptosis of LNCap cells. (A) Western blot analyses of Bcl-2 expression in different groups. (B) Caspase-3 activity assay in different groups. * P<0.05 compared with miRNA group. # P<0.05 compared with miR-143 group.

Article Snippet: The prostate cancer LNCap cells were purchased from American Type Culture Collection (ATCC, Rockville, Maryland, USA).

Techniques: Western Blot, Expressing, Caspase-3 Activity Assay

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: miR-143 Induces the Apoptosis of Prostate Cancer LNCap Cells by Suppressing Bcl-2 Expression

doi: 10.12659/MSM.899719

Figure Lengend Snippet: Bcl-2 overexpression inhibited the miR-143-induced apoptosis of LNCap cells. (A) Western blot analyses of Bcl-2 expression in different groups. (B) Caspase-3 activity assay in different groups. * P<0.05 compared with miRNA group. # P<0.05 compared with miR-143 group.

Article Snippet: The prostate cancer LNCap cells were purchased from American Type Culture Collection (ATCC, Rockville, Maryland, USA).

Techniques: Over Expression, Western Blot, Expressing, Caspase-3 Activity Assay

Journal: BioMed Research International

Article Title: Simultaneous Quantification of Mitochondrial DNA Damage and Copy Number in Circulating Blood: A Sensitive Approach to Systemic Oxidative Stress

doi: 10.1155/2013/157547

Figure Lengend Snippet: Absolute quantification of total mtDNA and baseline mtDNA damage in lymphocytes and prostate cancer cells. C4-2 ( n = 2), LNCaP ( n = 2), and lymphocytes ( n = 4) were analyzed by ss-qPCR for total mtDNA content, damaged mtDNA number, and level of baseline damage. The cell number was calculated from the copy numbers of calicin, a single copy nuclear marker. (a) With mtDNA CO2 marker, the original (CO2 original) and preheated (CO2-heated) DNA templates were quantified for damaged copies and total mtDNA copies, respectively. (b) The baseline damage level was obtained by dividing damaged copies over total copies. (c) Comparison of CO2 versus D-loop markers in lymphocyte samples ( n = 4).

Article Snippet: Prostate cancer cell line LNCaP was purchased from ATCC (Manassas, VA).

Techniques: Marker