Structured Review

Waters Corporation liquid chromatography tandem mass spectrometry
Exhaled breath (EB) samples tested by reversed‐phase <t>liquid</t> <t>chromatography/tandem</t> <t>mass</t> <t>spectrometry</t> for 12 model compounds including anabolic agents, hormone and metabolic modulators, stimulants, and beta‐blockers: (A) blank EB specimen containing only the internal standards D 3 ‐testosterone, D 3 ‐meldonium, ( S )‐2‐aminooctane, and D 7 ‐propranolol. Y‐axes are normalized to the abundance of the corresponding spiked specimen shown under (B), which illustrates the results of an EB sample fortified with 500 pg of each target analyte plus ISTDs
Liquid Chromatography Tandem Mass Spectrometry, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 93/100, based on 92 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/liquid chromatography tandem mass spectrometry/product/Waters Corporation
Average 93 stars, based on 92 article reviews
Price from $9.99 to $1999.99
liquid chromatography tandem mass spectrometry - by Bioz Stars, 2020-07
93/100 stars

Images

1) Product Images from "Expanding analytical options in sports drug testing: Mass spectrometric detection of prohibited substances in exhaled breath. Expanding analytical options in sports drug testing: Mass spectrometric detection of prohibited substances in exhaled breath"

Article Title: Expanding analytical options in sports drug testing: Mass spectrometric detection of prohibited substances in exhaled breath. Expanding analytical options in sports drug testing: Mass spectrometric detection of prohibited substances in exhaled breath

Journal: Rapid Communications in Mass Spectrometry

doi: 10.1002/rcm.7903

Exhaled breath (EB) samples tested by reversed‐phase liquid chromatography/tandem mass spectrometry for 12 model compounds including anabolic agents, hormone and metabolic modulators, stimulants, and beta‐blockers: (A) blank EB specimen containing only the internal standards D 3 ‐testosterone, D 3 ‐meldonium, ( S )‐2‐aminooctane, and D 7 ‐propranolol. Y‐axes are normalized to the abundance of the corresponding spiked specimen shown under (B), which illustrates the results of an EB sample fortified with 500 pg of each target analyte plus ISTDs
Figure Legend Snippet: Exhaled breath (EB) samples tested by reversed‐phase liquid chromatography/tandem mass spectrometry for 12 model compounds including anabolic agents, hormone and metabolic modulators, stimulants, and beta‐blockers: (A) blank EB specimen containing only the internal standards D 3 ‐testosterone, D 3 ‐meldonium, ( S )‐2‐aminooctane, and D 7 ‐propranolol. Y‐axes are normalized to the abundance of the corresponding spiked specimen shown under (B), which illustrates the results of an EB sample fortified with 500 pg of each target analyte plus ISTDs

Techniques Used: Liquid Chromatography, Mass Spectrometry

2) Product Images from "2′-(2-bromohexadecanoyl)-paclitaxel conjugate nanoparticles for the treatment of non-small cell lung cancer in an orthotopic xenograft mouse model"

Article Title: 2′-(2-bromohexadecanoyl)-paclitaxel conjugate nanoparticles for the treatment of non-small cell lung cancer in an orthotopic xenograft mouse model

Journal: International Journal of Nanomedicine

doi: 10.2147/IJN.S66040

Pharmacokinetic and biodistribution studies in an orthotopic NSCLC model (3 mice per time point). Notes: ( A ) and ( B ), PK profile of Br-C16-PX NPs and Taxol in plasma ( A ) and tumor-bearing lungs ( B ). Drug concentrations of PX from Taxol (open circle), Br-C16-PX from NPs (triangle) and PX converted from Br-C16-PX NPs (square) were quantified by LC-MS/MS. ( C ) Biodistribution of Br-C16-PX in plasma, liver, spleen, lung, kidney, and pleural fluid at predetermined time points. ( D ) Ratio of converted PX to Br-C16-PX in different tissues. Ratio = (molar concentration of PX converted from Br-C16-PX)/(molar concentration of Br-C16-PX). Abbreviations: Conc, concentration; PK, pharmacokinetic; Br-C16-PX, 2′-(2-bromohexadecanoyl)-paclitaxel; NPs, nanoparticles; LC-MS/MS, liquid chromatography-tandem mass spectrometry (MS/MS); PX, paclitaxel; NSCLC, non-small cell lung cancer.
Figure Legend Snippet: Pharmacokinetic and biodistribution studies in an orthotopic NSCLC model (3 mice per time point). Notes: ( A ) and ( B ), PK profile of Br-C16-PX NPs and Taxol in plasma ( A ) and tumor-bearing lungs ( B ). Drug concentrations of PX from Taxol (open circle), Br-C16-PX from NPs (triangle) and PX converted from Br-C16-PX NPs (square) were quantified by LC-MS/MS. ( C ) Biodistribution of Br-C16-PX in plasma, liver, spleen, lung, kidney, and pleural fluid at predetermined time points. ( D ) Ratio of converted PX to Br-C16-PX in different tissues. Ratio = (molar concentration of PX converted from Br-C16-PX)/(molar concentration of Br-C16-PX). Abbreviations: Conc, concentration; PK, pharmacokinetic; Br-C16-PX, 2′-(2-bromohexadecanoyl)-paclitaxel; NPs, nanoparticles; LC-MS/MS, liquid chromatography-tandem mass spectrometry (MS/MS); PX, paclitaxel; NSCLC, non-small cell lung cancer.

Techniques Used: Mouse Assay, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Concentration Assay, Liquid Chromatography

3) Product Images from "Correlation between radioactivity and chemotherapeutics of the 111In-VNB-liposome in pharmacokinetics and biodistribution in rats"

Article Title: Correlation between radioactivity and chemotherapeutics of the 111In-VNB-liposome in pharmacokinetics and biodistribution in rats

Journal: International Journal of Nanomedicine

doi: 10.2147/IJN.S28279

Typical liquid chromatography tandem mass spectrometry images of vinorelbine. ( A ) Blank plasma sample, ( B ) blank plasma spiked vinorelbine sample (200 ng/mL), ( C ) real plasma sample (156 ng/mL, t = 24 hours), ( D ) blank liver sample, ( E ) blank liver spiked vinorelbine sample (200 ng/mL), ( F ) real liver sample (141 ng/mL, t = 4 hours), ( G ) blank spleen sample, ( H ) blank spleen spiked vinorelbine sample (500 ng/mL), ( I ) real spleen sample (424 ng/mL, t = 1 hour). Note: The maximal signal intensities for plasma, liver and spleen samples were 9.0 × e5, 1.3 × e6, and 8.5 × e5, respectively.
Figure Legend Snippet: Typical liquid chromatography tandem mass spectrometry images of vinorelbine. ( A ) Blank plasma sample, ( B ) blank plasma spiked vinorelbine sample (200 ng/mL), ( C ) real plasma sample (156 ng/mL, t = 24 hours), ( D ) blank liver sample, ( E ) blank liver spiked vinorelbine sample (200 ng/mL), ( F ) real liver sample (141 ng/mL, t = 4 hours), ( G ) blank spleen sample, ( H ) blank spleen spiked vinorelbine sample (500 ng/mL), ( I ) real spleen sample (424 ng/mL, t = 1 hour). Note: The maximal signal intensities for plasma, liver and spleen samples were 9.0 × e5, 1.3 × e6, and 8.5 × e5, respectively.

Techniques Used: Liquid Chromatography, Mass Spectrometry

4) Product Images from "Quantitative proteomics links the LRRC59 interactome to mRNA translation on the ER membrane"

Article Title: Quantitative proteomics links the LRRC59 interactome to mRNA translation on the ER membrane

Journal: bioRxiv

doi: 10.1101/2020.03.04.975474

Proximity proteomics reveals unique interactomes for each of the four tested baits. (A) Schematic of the experimental approach. BirA-reporters for known (SEC61β (purple), RPN1 (green)) and candidate (SEC62 (orange), LRRC59 (blue)) ER-resident ribosome interacting proteins were expressed with biotin labeling (3 hours) conducted in biological triplicate. An empty vector negative control (red) was included. Samples were digested, tandem mass tag (TMT) labeled, and combined for liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Enrichment analyses of biotin-labeled proteins will reveal protein-protein interactions and/or functional networks for each of the five baits. (B) Violin plots of the protein abundance distributions for all biotin-labeled proteins (n=1,263) for each bait. (C) Clustered heatmap showing the average log 2 FC (across biological replicates) for each of 1,263 identified proteins per bait (green represents enriched protein abundance; red indicates decreased protein abundance). Boxplots showing the enrichment of highly enriched proteins (prey) labeled in the (D) SEC61β, (E) RPN1, (F) SEC62, and (G) LRRC59 BioID reporter studies. Each dot represents the log 2 FC value per biological replicate.
Figure Legend Snippet: Proximity proteomics reveals unique interactomes for each of the four tested baits. (A) Schematic of the experimental approach. BirA-reporters for known (SEC61β (purple), RPN1 (green)) and candidate (SEC62 (orange), LRRC59 (blue)) ER-resident ribosome interacting proteins were expressed with biotin labeling (3 hours) conducted in biological triplicate. An empty vector negative control (red) was included. Samples were digested, tandem mass tag (TMT) labeled, and combined for liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Enrichment analyses of biotin-labeled proteins will reveal protein-protein interactions and/or functional networks for each of the five baits. (B) Violin plots of the protein abundance distributions for all biotin-labeled proteins (n=1,263) for each bait. (C) Clustered heatmap showing the average log 2 FC (across biological replicates) for each of 1,263 identified proteins per bait (green represents enriched protein abundance; red indicates decreased protein abundance). Boxplots showing the enrichment of highly enriched proteins (prey) labeled in the (D) SEC61β, (E) RPN1, (F) SEC62, and (G) LRRC59 BioID reporter studies. Each dot represents the log 2 FC value per biological replicate.

Techniques Used: Labeling, Plasmid Preparation, Negative Control, Liquid Chromatography, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy, Functional Assay

Related Articles

High Performance Liquid Chromatography:

Article Title: Toward personalized hemodialysis by low molecular weight amino-containing compounds: future perspective of patient metabolic fingerprint
Article Snippet: .. The DIMS analysis for the evaluation of metabolite profile, specifically carnitine species and amino acids, in plasma samples was performed using a Liquid Chromatography-tandem Mass Spectrometry (LC/MS/MS) system consisting of an Alliance HT 2795 HPLC Separation Module coupled to a Quattro Ultima Pt ESI tandem quadrupole mass spectrometer (Waters Corporation, Milford, MA, USA). .. The instrument operated in positive electrospray ionisation mode using MassLynx V4.0 Software (Waters) with auto data processing by NeoLynx (Waters Corporation, Milford, MA, USA).

Cell Surface Hydrophobicity:

Article Title: 2′-(2-bromohexadecanoyl)-paclitaxel conjugate nanoparticles for the treatment of non-small cell lung cancer in an orthotopic xenograft mouse model
Article Snippet: .. Quantification of PX and Br-C16-PX by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS) PX, Br-C16-PX and docetaxel (internal standard) were separated on a Waters XSelect CSH Phenyl-Hexyl column (2.1×50 mm, 130 Å pore size, 5 μm particle size; Waters Corporation, Milford, MA, USA) using a gradient mobile phase consisting of 0.1% formic acid in water (mobile phase A) and 0.1% formic acid and 10% isopropanol in acetonitrile (mobile phase B) on a Shimadzu LC-20AD (Shimadzu, Columbia, MD) liquid chromatography. ..

Liquid Chromatography:

Article Title: 2′-(2-bromohexadecanoyl)-paclitaxel conjugate nanoparticles for the treatment of non-small cell lung cancer in an orthotopic xenograft mouse model
Article Snippet: .. Quantification of PX and Br-C16-PX by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS) PX, Br-C16-PX and docetaxel (internal standard) were separated on a Waters XSelect CSH Phenyl-Hexyl column (2.1×50 mm, 130 Å pore size, 5 μm particle size; Waters Corporation, Milford, MA, USA) using a gradient mobile phase consisting of 0.1% formic acid in water (mobile phase A) and 0.1% formic acid and 10% isopropanol in acetonitrile (mobile phase B) on a Shimadzu LC-20AD (Shimadzu, Columbia, MD) liquid chromatography. ..

Article Title: Novel Serum Biomarkers to Differentiate Cholangiocarcinoma from Benign Biliary Tract Diseases Using a Proteomic Approach
Article Snippet: .. Liquid Chromatography-Tandem Mass Spectrometry (LC/MS-MS) The LC-MS/MS analysis was carried out using a Waters nanoACQUITY ultra performance liquid chromatography coupled with a SYNAPT HDMS mass spectrometer. .. A 5-μ L aliquot of peptide fractions was injected using a built-in nanoACQUITY auto sampler onto a Symmetry C18 trapping column (200 μ m × 180 mm, 5 μ m particle size; Waters) at 10 μ L/min flow rate for on-line desalting and then separated on a C-18 RP nano-BEH column (75 μ m id × 200 mm, 1.7 μ m particle size, Waters) and eluted in a 30 min gradient of 2% to 40% ACN in 0.1% formic acid (FA) at 350 nL/min, followed by a 10-min ramping to 80% ACN-0.1% FA and a 5-min holding at 80% ACN-0.1% FA.

Article Title: Quantitative proteomics links the LRRC59 interactome to mRNA translation on the ER membrane
Article Snippet: .. Liquid chromatography – tandem mass spectrometry Approximately 1 µg of TMT-labeled peptide from each fraction was analyzed by nanoscale liquid chromatography – tandem mass spectrometry (LC-MS/MS) on a nanoAquity UPLC (Waters) coupled to an Orbitrap Fusion Lumos Tribrid mass spectrometer (ThermoFisher Scientific). .. Peptides were first trapped on a column at 99.9% water and 5 µL/min, followed by separation at 0.4 µL/min on an analytical column (Waters Corporation) with a gradient from 3 to 30% MeCN (0.1% formic acid) over 90 minutes.

Article Title: Expanding analytical options in sports drug testing: Mass spectrometric detection of prohibited substances in exhaled breath. Expanding analytical options in sports drug testing: Mass spectrometric detection of prohibited substances in exhaled breath
Article Snippet: .. 2.2 Liquid chromatography/tandem mass spectrometry (LC/MS/MS) All analyses were conducted using a Aquity I‐Class ultra‐performance liquid chromatography (UPLC) system (Waters, Eschborn, Germany) interfaced via unispray (US) ionization to a Xevo TQ‐XS tandem mass spectrometer (Waters). .. The LC system was equipped with a Poroshell C‐8 analytical column (50 × 3.0 mm, 2.7 μm particle size; Agilent, Waldbronn, Germany).

Mass Spectrometry:

Article Title: 2′-(2-bromohexadecanoyl)-paclitaxel conjugate nanoparticles for the treatment of non-small cell lung cancer in an orthotopic xenograft mouse model
Article Snippet: .. Quantification of PX and Br-C16-PX by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS) PX, Br-C16-PX and docetaxel (internal standard) were separated on a Waters XSelect CSH Phenyl-Hexyl column (2.1×50 mm, 130 Å pore size, 5 μm particle size; Waters Corporation, Milford, MA, USA) using a gradient mobile phase consisting of 0.1% formic acid in water (mobile phase A) and 0.1% formic acid and 10% isopropanol in acetonitrile (mobile phase B) on a Shimadzu LC-20AD (Shimadzu, Columbia, MD) liquid chromatography. ..

Article Title: Novel Serum Biomarkers to Differentiate Cholangiocarcinoma from Benign Biliary Tract Diseases Using a Proteomic Approach
Article Snippet: .. Liquid Chromatography-Tandem Mass Spectrometry (LC/MS-MS) The LC-MS/MS analysis was carried out using a Waters nanoACQUITY ultra performance liquid chromatography coupled with a SYNAPT HDMS mass spectrometer. .. A 5-μ L aliquot of peptide fractions was injected using a built-in nanoACQUITY auto sampler onto a Symmetry C18 trapping column (200 μ m × 180 mm, 5 μ m particle size; Waters) at 10 μ L/min flow rate for on-line desalting and then separated on a C-18 RP nano-BEH column (75 μ m id × 200 mm, 1.7 μ m particle size, Waters) and eluted in a 30 min gradient of 2% to 40% ACN in 0.1% formic acid (FA) at 350 nL/min, followed by a 10-min ramping to 80% ACN-0.1% FA and a 5-min holding at 80% ACN-0.1% FA.

Article Title: Vitamin D and Cognitive Function and Dementia Risk in a Biracial Cohort: the ARIC Brain MRI Study
Article Snippet: .. 25(OH)D was measured in 2012 from serum samples that were stored at −70°C since collection during visit 3 (1993–1995) using liquid chromatography-tandem mass spectrometry (Waters Alliance e2795, Milford, Massachusetts). .. The inter-assay coefficient of variations (CVs) for 25(OH)D2 are 6.2% and 5.3% at concentrations of 7.9 and 12.9 ng/mL, respectively.

Article Title: Anti-Inflammatory and Antioxidant Properties of Peptides Released from β-Lactoglobulin by High Hydrostatic Pressure-Assisted Enzymatic Hydrolysis
Article Snippet: .. Liquid Chromatography-Tandem Mass Spectrometry (LC–MS/MS) The hydrolysate was subjected to LC-MS/MS analysis on a nano UPLC (Waters, Milford, MA, USA), coupled with a q-Tof premier mass spectrometer (Waters, Milford, MA, USA). .. Five µL of the peptide mixture was loaded onto a nanoAcquity UPLC system, with a C18 PepMap 100 Nano-Precolumn™ (300 µm × 1 mm, Dionex, Sunnyvale, CA, USA), and a nano analytical column (75 µm × 150 mm, C18 acclaim PepMap 100 column™, Dionex, Sunnyvale, CA, USA).

Article Title: Quantitative proteomics links the LRRC59 interactome to mRNA translation on the ER membrane
Article Snippet: .. Liquid chromatography – tandem mass spectrometry Approximately 1 µg of TMT-labeled peptide from each fraction was analyzed by nanoscale liquid chromatography – tandem mass spectrometry (LC-MS/MS) on a nanoAquity UPLC (Waters) coupled to an Orbitrap Fusion Lumos Tribrid mass spectrometer (ThermoFisher Scientific). .. Peptides were first trapped on a column at 99.9% water and 5 µL/min, followed by separation at 0.4 µL/min on an analytical column (Waters Corporation) with a gradient from 3 to 30% MeCN (0.1% formic acid) over 90 minutes.

Article Title: Expanding analytical options in sports drug testing: Mass spectrometric detection of prohibited substances in exhaled breath. Expanding analytical options in sports drug testing: Mass spectrometric detection of prohibited substances in exhaled breath
Article Snippet: .. 2.2 Liquid chromatography/tandem mass spectrometry (LC/MS/MS) All analyses were conducted using a Aquity I‐Class ultra‐performance liquid chromatography (UPLC) system (Waters, Eschborn, Germany) interfaced via unispray (US) ionization to a Xevo TQ‐XS tandem mass spectrometer (Waters). .. The LC system was equipped with a Poroshell C‐8 analytical column (50 × 3.0 mm, 2.7 μm particle size; Agilent, Waldbronn, Germany).

Article Title: Toward personalized hemodialysis by low molecular weight amino-containing compounds: future perspective of patient metabolic fingerprint
Article Snippet: .. The DIMS analysis for the evaluation of metabolite profile, specifically carnitine species and amino acids, in plasma samples was performed using a Liquid Chromatography-tandem Mass Spectrometry (LC/MS/MS) system consisting of an Alliance HT 2795 HPLC Separation Module coupled to a Quattro Ultima Pt ESI tandem quadrupole mass spectrometer (Waters Corporation, Milford, MA, USA). .. The instrument operated in positive electrospray ionisation mode using MassLynx V4.0 Software (Waters) with auto data processing by NeoLynx (Waters Corporation, Milford, MA, USA).

Article Title: Correlation between radioactivity and chemotherapeutics of the 111In-VNB-liposome in pharmacokinetics and biodistribution in rats
Article Snippet: .. Liquid chromatography-tandem mass spectrometry The system consisted of a Waters 2690 Alliance LC with an automatic liquid chromatographic sampler and injector and a Micromass Quattro Ultima tandem quadrupole mass spectrometer (Micromass, Manchester, UK) equipped with an electrospray ionization interface which was in acquired positive mode. ..

Chromatography:

Article Title: Novel Serum Biomarkers to Differentiate Cholangiocarcinoma from Benign Biliary Tract Diseases Using a Proteomic Approach
Article Snippet: .. Liquid Chromatography-Tandem Mass Spectrometry (LC/MS-MS) The LC-MS/MS analysis was carried out using a Waters nanoACQUITY ultra performance liquid chromatography coupled with a SYNAPT HDMS mass spectrometer. .. A 5-μ L aliquot of peptide fractions was injected using a built-in nanoACQUITY auto sampler onto a Symmetry C18 trapping column (200 μ m × 180 mm, 5 μ m particle size; Waters) at 10 μ L/min flow rate for on-line desalting and then separated on a C-18 RP nano-BEH column (75 μ m id × 200 mm, 1.7 μ m particle size, Waters) and eluted in a 30 min gradient of 2% to 40% ACN in 0.1% formic acid (FA) at 350 nL/min, followed by a 10-min ramping to 80% ACN-0.1% FA and a 5-min holding at 80% ACN-0.1% FA.

Article Title: Vitamin D and Cognitive Function and Dementia Risk in a Biracial Cohort: the ARIC Brain MRI Study
Article Snippet: .. 25(OH)D was measured in 2012 from serum samples that were stored at −70°C since collection during visit 3 (1993–1995) using liquid chromatography-tandem mass spectrometry (Waters Alliance e2795, Milford, Massachusetts). .. The inter-assay coefficient of variations (CVs) for 25(OH)D2 are 6.2% and 5.3% at concentrations of 7.9 and 12.9 ng/mL, respectively.

Article Title: Anti-Inflammatory and Antioxidant Properties of Peptides Released from β-Lactoglobulin by High Hydrostatic Pressure-Assisted Enzymatic Hydrolysis
Article Snippet: .. Liquid Chromatography-Tandem Mass Spectrometry (LC–MS/MS) The hydrolysate was subjected to LC-MS/MS analysis on a nano UPLC (Waters, Milford, MA, USA), coupled with a q-Tof premier mass spectrometer (Waters, Milford, MA, USA). .. Five µL of the peptide mixture was loaded onto a nanoAcquity UPLC system, with a C18 PepMap 100 Nano-Precolumn™ (300 µm × 1 mm, Dionex, Sunnyvale, CA, USA), and a nano analytical column (75 µm × 150 mm, C18 acclaim PepMap 100 column™, Dionex, Sunnyvale, CA, USA).

Article Title: Expanding analytical options in sports drug testing: Mass spectrometric detection of prohibited substances in exhaled breath. Expanding analytical options in sports drug testing: Mass spectrometric detection of prohibited substances in exhaled breath
Article Snippet: .. 2.2 Liquid chromatography/tandem mass spectrometry (LC/MS/MS) All analyses were conducted using a Aquity I‐Class ultra‐performance liquid chromatography (UPLC) system (Waters, Eschborn, Germany) interfaced via unispray (US) ionization to a Xevo TQ‐XS tandem mass spectrometer (Waters). .. The LC system was equipped with a Poroshell C‐8 analytical column (50 × 3.0 mm, 2.7 μm particle size; Agilent, Waldbronn, Germany).

Article Title: Toward personalized hemodialysis by low molecular weight amino-containing compounds: future perspective of patient metabolic fingerprint
Article Snippet: .. The DIMS analysis for the evaluation of metabolite profile, specifically carnitine species and amino acids, in plasma samples was performed using a Liquid Chromatography-tandem Mass Spectrometry (LC/MS/MS) system consisting of an Alliance HT 2795 HPLC Separation Module coupled to a Quattro Ultima Pt ESI tandem quadrupole mass spectrometer (Waters Corporation, Milford, MA, USA). .. The instrument operated in positive electrospray ionisation mode using MassLynx V4.0 Software (Waters) with auto data processing by NeoLynx (Waters Corporation, Milford, MA, USA).

Article Title: Correlation between radioactivity and chemotherapeutics of the 111In-VNB-liposome in pharmacokinetics and biodistribution in rats
Article Snippet: .. Liquid chromatography-tandem mass spectrometry The system consisted of a Waters 2690 Alliance LC with an automatic liquid chromatographic sampler and injector and a Micromass Quattro Ultima tandem quadrupole mass spectrometer (Micromass, Manchester, UK) equipped with an electrospray ionization interface which was in acquired positive mode. ..

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Waters Corporation sim lc ms ms
    Analysis of <t>HiPMO1</t> reaction products hydrolyzed by beta-glucuronidase and beta-glucosidase using <t>SIM</t> LC–MS/MS analysis. a SIM LC–MS showing extracted ion chromatograms and the corresponding mass spectra of glucuronic acid and saccharic acid (saccharic acid lactone). In positive mode: saccharic acid ( m / z 210 + H + ). In negative mode: glucuronic acid ( m / z 194 − H + ) and saccharic acid lactone ( m / z 192 − H + ). b The SIM MS/MS spectra showing fragmentation ions of the parent ions at m / z 192.8 for glucuronic acid ( m / z 194 − H + ) and at m / z 190.9 for saccharic acid lactone ( m / z 192 − H + ). Loss of [H] and [H 2 O] and addition of [H] is common in carbohydrate fragmentations using LC–MS/MS in the negative ion mode [ 38 ]. The loss of [H], [H 2 O], [CHO], and [COOH] and the addition of [H] from the parent ions of glucuronic acid ( m/z 194 − H + ) and saccharic acid lactone ( m / z 192 − H + ), generating various fragmentation ions. The MS/MS spectra of saccharic acid ( m / z 210 + H + ) were not shown, likely due to the low intensities
    Sim Lc Ms Ms, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sim lc ms ms/product/Waters Corporation
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sim lc ms ms - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    90
    Waters Corporation γ hbcdd
    Analytical scale LC-MS separation of ( A ) a technical <t>HBCDD</t> mixture and ( B ) the isolated α-, β-, and <t>γ-HBCDD</t> enantiomers as well as reference standards for δ- and ε-HBCDD on a Phenomenex Nucleodex β-PM (permethylated β-cyclodextrin) stationary phase at ambient temperature using an acetonitrile/water mobile phase.
    γ Hbcdd, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/γ hbcdd/product/Waters Corporation
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    γ hbcdd - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    92
    Waters Corporation waters xbridge c18
    Extracted ion chromatograms of 31 standard compounds separated by reverse-phase chromatography using a (A) <t>XBridge</t> <t>C18,</t> (B) Cogent Bidentate C18, and (C) Scherzo SM-C18 column.
    Waters Xbridge C18, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 92/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/waters xbridge c18/product/Waters Corporation
    Average 92 stars, based on 94 article reviews
    Price from $9.99 to $1999.99
    waters xbridge c18 - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    85
    Waters Corporation urinary vitamin e metabolites
    Product ion scans of <t>vitamin</t> E metabolites.
    Urinary Vitamin E Metabolites, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/urinary vitamin e metabolites/product/Waters Corporation
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    urinary vitamin e metabolites - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    Image Search Results


    Analysis of HiPMO1 reaction products hydrolyzed by beta-glucuronidase and beta-glucosidase using SIM LC–MS/MS analysis. a SIM LC–MS showing extracted ion chromatograms and the corresponding mass spectra of glucuronic acid and saccharic acid (saccharic acid lactone). In positive mode: saccharic acid ( m / z 210 + H + ). In negative mode: glucuronic acid ( m / z 194 − H + ) and saccharic acid lactone ( m / z 192 − H + ). b The SIM MS/MS spectra showing fragmentation ions of the parent ions at m / z 192.8 for glucuronic acid ( m / z 194 − H + ) and at m / z 190.9 for saccharic acid lactone ( m / z 192 − H + ). Loss of [H] and [H 2 O] and addition of [H] is common in carbohydrate fragmentations using LC–MS/MS in the negative ion mode [ 38 ]. The loss of [H], [H 2 O], [CHO], and [COOH] and the addition of [H] from the parent ions of glucuronic acid ( m/z 194 − H + ) and saccharic acid lactone ( m / z 192 − H + ), generating various fragmentation ions. The MS/MS spectra of saccharic acid ( m / z 210 + H + ) were not shown, likely due to the low intensities

    Journal: Biotechnology for Biofuels

    Article Title: Polysaccharide monooxygenase-catalyzed oxidation of cellulose to glucuronic acid-containing cello-oligosaccharides

    doi: 10.1186/s13068-019-1384-0

    Figure Lengend Snippet: Analysis of HiPMO1 reaction products hydrolyzed by beta-glucuronidase and beta-glucosidase using SIM LC–MS/MS analysis. a SIM LC–MS showing extracted ion chromatograms and the corresponding mass spectra of glucuronic acid and saccharic acid (saccharic acid lactone). In positive mode: saccharic acid ( m / z 210 + H + ). In negative mode: glucuronic acid ( m / z 194 − H + ) and saccharic acid lactone ( m / z 192 − H + ). b The SIM MS/MS spectra showing fragmentation ions of the parent ions at m / z 192.8 for glucuronic acid ( m / z 194 − H + ) and at m / z 190.9 for saccharic acid lactone ( m / z 192 − H + ). Loss of [H] and [H 2 O] and addition of [H] is common in carbohydrate fragmentations using LC–MS/MS in the negative ion mode [ 38 ]. The loss of [H], [H 2 O], [CHO], and [COOH] and the addition of [H] from the parent ions of glucuronic acid ( m/z 194 − H + ) and saccharic acid lactone ( m / z 192 − H + ), generating various fragmentation ions. The MS/MS spectra of saccharic acid ( m / z 210 + H + ) were not shown, likely due to the low intensities

    Article Snippet: The full scan m /z ranged from 100 to 300. (ii) SIM LC–MS/MS: We analyzed HiPMO1 reaction products using SIM LC–MS (ACQUITY UPLC and Q-TOF MS Premier, Waters) on a U3000-HPLC C18 column (Agilent Zorbax SB-C18, 150 × 4.6 mm).

    Techniques: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry

    Analytical scale LC-MS separation of ( A ) a technical HBCDD mixture and ( B ) the isolated α-, β-, and γ-HBCDD enantiomers as well as reference standards for δ- and ε-HBCDD on a Phenomenex Nucleodex β-PM (permethylated β-cyclodextrin) stationary phase at ambient temperature using an acetonitrile/water mobile phase.

    Journal: Molecules

    Article Title: Enantioselective Analytical- and Preparative-Scale Separation of Hexabromocyclododecane Stereoisomers Using Packed Column Supercritical Fluid Chromatography

    doi: 10.3390/molecules21111509

    Figure Lengend Snippet: Analytical scale LC-MS separation of ( A ) a technical HBCDD mixture and ( B ) the isolated α-, β-, and γ-HBCDD enantiomers as well as reference standards for δ- and ε-HBCDD on a Phenomenex Nucleodex β-PM (permethylated β-cyclodextrin) stationary phase at ambient temperature using an acetonitrile/water mobile phase.

    Article Snippet: Isolation of Enantiomerically Pure (+)- and (−)-α-, β-, and γ-HBCDD by Preparative-Scale pSFC Preparative-scale pSFC separations were carried out using a Waters Prep15 SFC system (Waters Corp., Milford, MA, USA) coupled with a Waters 3100 benchtop single quadrupole mass detector (Waters Corp., Milford, MA, USA) configured in negative ion atmospheric pressure chemical ionization (APCI) mode with the following parameters: corona needle (uA) = 5.00, cone voltage (V) = 30.00; desolvation gas flow (L/h) = 550; desolvation temperature (°C) = 350; source temperature (°C) = 125.

    Techniques: Liquid Chromatography with Mass Spectroscopy, Isolation

    Analytical scale pSFC-MS separation of ( A ) a technical HBCDD mixture and ( B ) the isolated α-, β-, and γ-HBCDD enantiomers as well as reference standards for δ- and ε-HBCDD on a Trefoil CEL2 [cellulose tris-(3-chloro-4-methylphenylcarbamate)] stationary phase at 50 °C using an isopropanol modified carbon dioxide mobile phase.

    Journal: Molecules

    Article Title: Enantioselective Analytical- and Preparative-Scale Separation of Hexabromocyclododecane Stereoisomers Using Packed Column Supercritical Fluid Chromatography

    doi: 10.3390/molecules21111509

    Figure Lengend Snippet: Analytical scale pSFC-MS separation of ( A ) a technical HBCDD mixture and ( B ) the isolated α-, β-, and γ-HBCDD enantiomers as well as reference standards for δ- and ε-HBCDD on a Trefoil CEL2 [cellulose tris-(3-chloro-4-methylphenylcarbamate)] stationary phase at 50 °C using an isopropanol modified carbon dioxide mobile phase.

    Article Snippet: Isolation of Enantiomerically Pure (+)- and (−)-α-, β-, and γ-HBCDD by Preparative-Scale pSFC Preparative-scale pSFC separations were carried out using a Waters Prep15 SFC system (Waters Corp., Milford, MA, USA) coupled with a Waters 3100 benchtop single quadrupole mass detector (Waters Corp., Milford, MA, USA) configured in negative ion atmospheric pressure chemical ionization (APCI) mode with the following parameters: corona needle (uA) = 5.00, cone voltage (V) = 30.00; desolvation gas flow (L/h) = 550; desolvation temperature (°C) = 350; source temperature (°C) = 125.

    Techniques: Mass Spectrometry, Isolation, Modification

    Stable conformations, schematic representations, and stereoviews of the crystal structures (when acquired) of enantiomerically pure (+)-α-HBCDD, (+)-γ-HBCDD, and (+)-β-HBCDD as well as the two minor isomers δ-HBCDD and ε-HBCDD.

    Journal: Molecules

    Article Title: Enantioselective Analytical- and Preparative-Scale Separation of Hexabromocyclododecane Stereoisomers Using Packed Column Supercritical Fluid Chromatography

    doi: 10.3390/molecules21111509

    Figure Lengend Snippet: Stable conformations, schematic representations, and stereoviews of the crystal structures (when acquired) of enantiomerically pure (+)-α-HBCDD, (+)-γ-HBCDD, and (+)-β-HBCDD as well as the two minor isomers δ-HBCDD and ε-HBCDD.

    Article Snippet: Isolation of Enantiomerically Pure (+)- and (−)-α-, β-, and γ-HBCDD by Preparative-Scale pSFC Preparative-scale pSFC separations were carried out using a Waters Prep15 SFC system (Waters Corp., Milford, MA, USA) coupled with a Waters 3100 benchtop single quadrupole mass detector (Waters Corp., Milford, MA, USA) configured in negative ion atmospheric pressure chemical ionization (APCI) mode with the following parameters: corona needle (uA) = 5.00, cone voltage (V) = 30.00; desolvation gas flow (L/h) = 550; desolvation temperature (°C) = 350; source temperature (°C) = 125.

    Techniques:

    Extracted ion chromatograms of 31 standard compounds separated by reverse-phase chromatography using a (A) XBridge C18, (B) Cogent Bidentate C18, and (C) Scherzo SM-C18 column.

    Journal: Analytical Chemistry

    Article Title: Expanding Coverage of the Metabolome for Global Metabolite Profiling

    doi: 10.1021/ac102981k

    Figure Lengend Snippet: Extracted ion chromatograms of 31 standard compounds separated by reverse-phase chromatography using a (A) XBridge C18, (B) Cogent Bidentate C18, and (C) Scherzo SM-C18 column.

    Article Snippet: E.coli extractions and standard mixtures were separated using a Cogent Bidentate C18: 4 μm, 100Å, 150mm × 2.1 mm ID (Cat No. 40018-15P-2), a Waters XBridge C18: 3.5 μm, 135Å, 150mm × 1.0 mm ID (Part No. 186003128), or an Imtakt Scherzo SM-C18: 3 μm, 13nm, 150mm × 2mm ID (Prod.

    Techniques: Reversed-phase Chromatography

    Quantification of 36 standard compounds analyzed in negative ionization mode (ESI−) using three different additives in the mobile phase: 1mM ammonium fluoride, 5mM ammonium acetate, or 0.1% formic acid. Compounds were separated by reverse-phase chromatography using a XBridge C18 column. Fold values indicate the difference in intensity between ammonium fluoride and the closest mobile phase. The intensity scale of the X axis is log 10.

    Journal: Analytical Chemistry

    Article Title: Expanding Coverage of the Metabolome for Global Metabolite Profiling

    doi: 10.1021/ac102981k

    Figure Lengend Snippet: Quantification of 36 standard compounds analyzed in negative ionization mode (ESI−) using three different additives in the mobile phase: 1mM ammonium fluoride, 5mM ammonium acetate, or 0.1% formic acid. Compounds were separated by reverse-phase chromatography using a XBridge C18 column. Fold values indicate the difference in intensity between ammonium fluoride and the closest mobile phase. The intensity scale of the X axis is log 10.

    Article Snippet: E.coli extractions and standard mixtures were separated using a Cogent Bidentate C18: 4 μm, 100Å, 150mm × 2.1 mm ID (Cat No. 40018-15P-2), a Waters XBridge C18: 3.5 μm, 135Å, 150mm × 1.0 mm ID (Part No. 186003128), or an Imtakt Scherzo SM-C18: 3 μm, 13nm, 150mm × 2mm ID (Prod.

    Techniques: Reversed-phase Chromatography

    (A) Venn diagram representing the total number of features from LC/MS data of E.coli samples extracted using the method “Boiling water”, and analyzed using ammonium acetate or ammonium fluoride enriched mobile phases. (B) Quantification of 39 metabolites from E.coli analyzed using ammonium acetate and ammonium fluoride enriched mobile phases. The intensity scale of the X axis is log 2. Metabolites were separated by reverse-phase chromatography using an XBridge C18 column and detected in negative ionization mode (ESI−). Identification is based on accurate mass and MS/MS data. Fold values indicate the difference in intensity between ammonium fluoride and ammonium acetate. Examples of unique metabolites detected with ammonium fluoride are also represented. Data points and error bars represent mean intensity values and s.d. for three replicates.

    Journal: Analytical Chemistry

    Article Title: Expanding Coverage of the Metabolome for Global Metabolite Profiling

    doi: 10.1021/ac102981k

    Figure Lengend Snippet: (A) Venn diagram representing the total number of features from LC/MS data of E.coli samples extracted using the method “Boiling water”, and analyzed using ammonium acetate or ammonium fluoride enriched mobile phases. (B) Quantification of 39 metabolites from E.coli analyzed using ammonium acetate and ammonium fluoride enriched mobile phases. The intensity scale of the X axis is log 2. Metabolites were separated by reverse-phase chromatography using an XBridge C18 column and detected in negative ionization mode (ESI−). Identification is based on accurate mass and MS/MS data. Fold values indicate the difference in intensity between ammonium fluoride and ammonium acetate. Examples of unique metabolites detected with ammonium fluoride are also represented. Data points and error bars represent mean intensity values and s.d. for three replicates.

    Article Snippet: E.coli extractions and standard mixtures were separated using a Cogent Bidentate C18: 4 μm, 100Å, 150mm × 2.1 mm ID (Cat No. 40018-15P-2), a Waters XBridge C18: 3.5 μm, 135Å, 150mm × 1.0 mm ID (Part No. 186003128), or an Imtakt Scherzo SM-C18: 3 μm, 13nm, 150mm × 2mm ID (Prod.

    Techniques: Liquid Chromatography with Mass Spectroscopy, Reversed-phase Chromatography, Mass Spectrometry

    Product ion scans of vitamin E metabolites.

    Journal: Free Radical Biology & Medicine

    Article Title: Urinary conjugated ?-tocopheronolactone--a biomarker of oxidative stress in children with type 1 diabetes

    doi: 10.1016/j.freeradbiomed.2012.09.012

    Figure Lengend Snippet: Product ion scans of vitamin E metabolites.

    Article Snippet: The urinary vitamin E metabolites were desalted and/or separated before mass spectrometry using a Waters 2795XE high-performance liquid chromatography unit fitted with a 100 × 2.1-mm (5 μ) HyPURITY C8 column plus a guard column containing the same stationary phase (Phenomenex UK).

    Techniques:

    The LC–MS/MS analysis of vitamin E metabolites and internal standards.

    Journal: Free Radical Biology & Medicine

    Article Title: Urinary conjugated ?-tocopheronolactone--a biomarker of oxidative stress in children with type 1 diabetes

    doi: 10.1016/j.freeradbiomed.2012.09.012

    Figure Lengend Snippet: The LC–MS/MS analysis of vitamin E metabolites and internal standards.

    Article Snippet: The urinary vitamin E metabolites were desalted and/or separated before mass spectrometry using a Waters 2795XE high-performance liquid chromatography unit fitted with a 100 × 2.1-mm (5 μ) HyPURITY C8 column plus a guard column containing the same stationary phase (Phenomenex UK).

    Techniques: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry

    Parent ion scans of vitamin E metabolites.

    Journal: Free Radical Biology & Medicine

    Article Title: Urinary conjugated ?-tocopheronolactone--a biomarker of oxidative stress in children with type 1 diabetes

    doi: 10.1016/j.freeradbiomed.2012.09.012

    Figure Lengend Snippet: Parent ion scans of vitamin E metabolites.

    Article Snippet: The urinary vitamin E metabolites were desalted and/or separated before mass spectrometry using a Waters 2795XE high-performance liquid chromatography unit fitted with a 100 × 2.1-mm (5 μ) HyPURITY C8 column plus a guard column containing the same stationary phase (Phenomenex UK).

    Techniques: