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Quotient Bioresearch liquid chromatography tandem mass spectrometry
Liquid Chromatography Tandem Mass Spectrometry, supplied by Quotient Bioresearch, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/liquid chromatography tandem mass spectrometry/product/Quotient Bioresearch
Average 92 stars, based on 1 article reviews
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liquid chromatography tandem mass spectrometry - by Bioz Stars, 2020-07
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Chromatography:

Article Title: Evaluation of regional gastrointestinal absorption of edoxaban using the enterion capsule
Article Snippet: .. In study 2, blood samples were collected at predose, 0.25, 0.5, 0.75, 1, 1.5, 2, 4, 6, 8, 12, 16, 24, 30, and 48 hours postdose for treatment E, and at preactivation, 3, 6, 9, 12, 15, 18, 21, 24, 30, 36, 42, and 48 hours postactivation for treatment F. Determination of edoxaban concentrations in plasma samples was carried out using liquid chromatography/tandem mass spectrometry (Quotient Bioresearch, Inc., Rushden, United Kingdom). .. The validated linear calibration curve (with 1/x weighting) ranged from 1 ng/mL (lower limit of quantitation) to 500 ng/mL (upper limit of quantitation), and included 7 levels of nonzero standards.

Mass Spectrometry:

Article Title: Evaluation of regional gastrointestinal absorption of edoxaban using the enterion capsule
Article Snippet: .. In study 2, blood samples were collected at predose, 0.25, 0.5, 0.75, 1, 1.5, 2, 4, 6, 8, 12, 16, 24, 30, and 48 hours postdose for treatment E, and at preactivation, 3, 6, 9, 12, 15, 18, 21, 24, 30, 36, 42, and 48 hours postactivation for treatment F. Determination of edoxaban concentrations in plasma samples was carried out using liquid chromatography/tandem mass spectrometry (Quotient Bioresearch, Inc., Rushden, United Kingdom). .. The validated linear calibration curve (with 1/x weighting) ranged from 1 ng/mL (lower limit of quantitation) to 500 ng/mL (upper limit of quantitation), and included 7 levels of nonzero standards.

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    Quotient Bioresearch c netarsudil
    Metabolism of <t>netarsudil</t> by ocular tissues. (A) Metabolism of netarsudil in the presence of corneal tissue isolated from Dutch Belted rabbit ( n = 3), beagle dog ( n = 3), pig ( n = 3), cynomolgus monkey ( n = 4), and human ( n = 3) corneas. Corneal metabolism assays were initiated by adding netarsudil in assay buffer to wells containing individual corneal punches in assay buffer followed by incubation at 37°C. Samples were removed at specified time intervals and analyzed by HPLC to determine the percentage of netarsudil remaining at each time point. (B) Levels of netarsudil versus netarsudil-M1 in AH following topical ocular application of netarsudil 0.02% to Dutch Belted rabbits. Netarsudil was administered to both eyes of 3 male New Zealand rabbits in each of 4 groups. In groups 1 and 2, test article was dosed only once, and samples of AH were taken from each eye by paracentesis at 4 or 6 h postdose, respectively. In groups 3 and 4, test article was dosed once daily for 3 or 4 days, respectively, and samples of AH were taken 4 h after dosing. Levels of netarsudil and its metabolite netarsudil-M1 were measured in the samples by HPLC/mass spectrometry. AH, aqueous humor; HPLC, high performance liquid chromatography.
    C Netarsudil, supplied by Quotient Bioresearch, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c netarsudil/product/Quotient Bioresearch
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    c netarsudil - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    Quotient Bioresearch liquid chromatography tandem mass spectrometry
    Metabolism of <t>netarsudil</t> by ocular tissues. (A) Metabolism of netarsudil in the presence of corneal tissue isolated from Dutch Belted rabbit ( n = 3), beagle dog ( n = 3), pig ( n = 3), cynomolgus monkey ( n = 4), and human ( n = 3) corneas. Corneal metabolism assays were initiated by adding netarsudil in assay buffer to wells containing individual corneal punches in assay buffer followed by incubation at 37°C. Samples were removed at specified time intervals and analyzed by HPLC to determine the percentage of netarsudil remaining at each time point. (B) Levels of netarsudil versus netarsudil-M1 in AH following topical ocular application of netarsudil 0.02% to Dutch Belted rabbits. Netarsudil was administered to both eyes of 3 male New Zealand rabbits in each of 4 groups. In groups 1 and 2, test article was dosed only once, and samples of AH were taken from each eye by paracentesis at 4 or 6 h postdose, respectively. In groups 3 and 4, test article was dosed once daily for 3 or 4 days, respectively, and samples of AH were taken 4 h after dosing. Levels of netarsudil and its metabolite netarsudil-M1 were measured in the samples by HPLC/mass spectrometry. AH, aqueous humor; HPLC, high performance liquid chromatography.
    Liquid Chromatography Tandem Mass Spectrometry, supplied by Quotient Bioresearch, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/liquid chromatography tandem mass spectrometry/product/Quotient Bioresearch
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    liquid chromatography tandem mass spectrometry - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    Image Search Results


    Metabolism of netarsudil by ocular tissues. (A) Metabolism of netarsudil in the presence of corneal tissue isolated from Dutch Belted rabbit ( n = 3), beagle dog ( n = 3), pig ( n = 3), cynomolgus monkey ( n = 4), and human ( n = 3) corneas. Corneal metabolism assays were initiated by adding netarsudil in assay buffer to wells containing individual corneal punches in assay buffer followed by incubation at 37°C. Samples were removed at specified time intervals and analyzed by HPLC to determine the percentage of netarsudil remaining at each time point. (B) Levels of netarsudil versus netarsudil-M1 in AH following topical ocular application of netarsudil 0.02% to Dutch Belted rabbits. Netarsudil was administered to both eyes of 3 male New Zealand rabbits in each of 4 groups. In groups 1 and 2, test article was dosed only once, and samples of AH were taken from each eye by paracentesis at 4 or 6 h postdose, respectively. In groups 3 and 4, test article was dosed once daily for 3 or 4 days, respectively, and samples of AH were taken 4 h after dosing. Levels of netarsudil and its metabolite netarsudil-M1 were measured in the samples by HPLC/mass spectrometry. AH, aqueous humor; HPLC, high performance liquid chromatography.

    Journal: Journal of Ocular Pharmacology and Therapeutics

    Article Title: Discovery and Preclinical Development of Netarsudil, a Novel Ocular Hypotensive Agent for the Treatment of Glaucoma

    doi: 10.1089/jop.2017.0023

    Figure Lengend Snippet: Metabolism of netarsudil by ocular tissues. (A) Metabolism of netarsudil in the presence of corneal tissue isolated from Dutch Belted rabbit ( n = 3), beagle dog ( n = 3), pig ( n = 3), cynomolgus monkey ( n = 4), and human ( n = 3) corneas. Corneal metabolism assays were initiated by adding netarsudil in assay buffer to wells containing individual corneal punches in assay buffer followed by incubation at 37°C. Samples were removed at specified time intervals and analyzed by HPLC to determine the percentage of netarsudil remaining at each time point. (B) Levels of netarsudil versus netarsudil-M1 in AH following topical ocular application of netarsudil 0.02% to Dutch Belted rabbits. Netarsudil was administered to both eyes of 3 male New Zealand rabbits in each of 4 groups. In groups 1 and 2, test article was dosed only once, and samples of AH were taken from each eye by paracentesis at 4 or 6 h postdose, respectively. In groups 3 and 4, test article was dosed once daily for 3 or 4 days, respectively, and samples of AH were taken 4 h after dosing. Levels of netarsudil and its metabolite netarsudil-M1 were measured in the samples by HPLC/mass spectrometry. AH, aqueous humor; HPLC, high performance liquid chromatography.

    Article Snippet: 14 C-netarsudil and 3 H-AR-12286 were prepared by Quotient Bioresearch (Cambridgeshire, United Kingdom) by incorporating a 14 C label into the β position of the amino acid portion of netarsudil and a 3 H label at the C-5 of the isoquinolone, respectively.

    Techniques: Isolation, Incubation, High Performance Liquid Chromatography, Mass Spectrometry

    IOP-lowering effect of netarsudil versus AR-12286 in rabbits and monkeys. Netarsudil 0.04% or AR-12286 0.5% was administered once daily (AM) to 1 eye of each animal for 3 days, with the fellow untreated eye serving as the control. IOP was determined for both eyes before test article administration (time 0) and at times 1, 2, 4, 8, and 24 h (rabbits) or 4, 8, and 24 h (monkeys) after each morning dose on Day 1 and 3. (A) Change in IOP in the treated eye relative to the untreated contralateral eye in Dutch Belted rabbits ( n = 12/group). For netarsudil 0.04% and AR-12286 0.5%, IOP reductions were statistically significant ( P

    Journal: Journal of Ocular Pharmacology and Therapeutics

    Article Title: Discovery and Preclinical Development of Netarsudil, a Novel Ocular Hypotensive Agent for the Treatment of Glaucoma

    doi: 10.1089/jop.2017.0023

    Figure Lengend Snippet: IOP-lowering effect of netarsudil versus AR-12286 in rabbits and monkeys. Netarsudil 0.04% or AR-12286 0.5% was administered once daily (AM) to 1 eye of each animal for 3 days, with the fellow untreated eye serving as the control. IOP was determined for both eyes before test article administration (time 0) and at times 1, 2, 4, 8, and 24 h (rabbits) or 4, 8, and 24 h (monkeys) after each morning dose on Day 1 and 3. (A) Change in IOP in the treated eye relative to the untreated contralateral eye in Dutch Belted rabbits ( n = 12/group). For netarsudil 0.04% and AR-12286 0.5%, IOP reductions were statistically significant ( P

    Article Snippet: 14 C-netarsudil and 3 H-AR-12286 were prepared by Quotient Bioresearch (Cambridgeshire, United Kingdom) by incorporating a 14 C label into the β position of the amino acid portion of netarsudil and a 3 H label at the C-5 of the isoquinolone, respectively.

    Techniques:

    Disruption of actin stress fibers and focal adhesions by netarsudil versus other ROCK inhibitors. (A) Netarsudil dose-response in actin stress fiber assay. Primary PTM cells were incubated for 6 h in the presence of 0, 0.015, 0.138, or 1.2 μM netarsudil then fixed and stained with Alexa Fluor-488 phalloidin and Hoechst 33342 to reveal actin fibers and nuclei, respectively. Top panel : fluorescence images of stained cells. Bottom panel : False color images created by an automated, custom algorithm to identify stress fibers and calculate mean stress fiber length. (B) Netarsudil dose-response in focal adhesion assay. Immortalized HTM cells (TM-1) were incubated for 6 h in the presence of 0, 0.015, 0.138, or 1.2 μM netarsudil then fixed and stained with mouse anti-paxillin antibody/Alexa Fluor ® 488 goat-anti-mouse IgG and Hoechst 33342 to reveal focal adhesions and nuclei, respectively. Top panel : fluorescence images of stained cells. Bottom panel : False color images created by an automated, custom algorithm to identify focal adhesions and calculate the mean number of focal adhesions per cell. (C) Dose–response curves ( n = 4) for netarsudil, netarsudil-M1, AR-12286, Y-27632, and fasudil in the PTM actin stress fiber length assay. Mean stress fiber length is presented as a percentage of the mean length of stress fibers measured in untreated control cells. (D) Dose–response curves ( n = 4) for netarsudil, netarsudil-M1, AR-12286, Y-27632, and fasudil in the HTM focal adhesion assay. Mean number of focal adhesions per cell is presented as a percentage of the number of focal adhesions per cell measured in untreated control cells. HTM, human trabecular meshwork; PTM, porcine trabecular meshwork; ROCK, Rho-associated protein kinase.

    Journal: Journal of Ocular Pharmacology and Therapeutics

    Article Title: Discovery and Preclinical Development of Netarsudil, a Novel Ocular Hypotensive Agent for the Treatment of Glaucoma

    doi: 10.1089/jop.2017.0023

    Figure Lengend Snippet: Disruption of actin stress fibers and focal adhesions by netarsudil versus other ROCK inhibitors. (A) Netarsudil dose-response in actin stress fiber assay. Primary PTM cells were incubated for 6 h in the presence of 0, 0.015, 0.138, or 1.2 μM netarsudil then fixed and stained with Alexa Fluor-488 phalloidin and Hoechst 33342 to reveal actin fibers and nuclei, respectively. Top panel : fluorescence images of stained cells. Bottom panel : False color images created by an automated, custom algorithm to identify stress fibers and calculate mean stress fiber length. (B) Netarsudil dose-response in focal adhesion assay. Immortalized HTM cells (TM-1) were incubated for 6 h in the presence of 0, 0.015, 0.138, or 1.2 μM netarsudil then fixed and stained with mouse anti-paxillin antibody/Alexa Fluor ® 488 goat-anti-mouse IgG and Hoechst 33342 to reveal focal adhesions and nuclei, respectively. Top panel : fluorescence images of stained cells. Bottom panel : False color images created by an automated, custom algorithm to identify focal adhesions and calculate the mean number of focal adhesions per cell. (C) Dose–response curves ( n = 4) for netarsudil, netarsudil-M1, AR-12286, Y-27632, and fasudil in the PTM actin stress fiber length assay. Mean stress fiber length is presented as a percentage of the mean length of stress fibers measured in untreated control cells. (D) Dose–response curves ( n = 4) for netarsudil, netarsudil-M1, AR-12286, Y-27632, and fasudil in the HTM focal adhesion assay. Mean number of focal adhesions per cell is presented as a percentage of the number of focal adhesions per cell measured in untreated control cells. HTM, human trabecular meshwork; PTM, porcine trabecular meshwork; ROCK, Rho-associated protein kinase.

    Article Snippet: 14 C-netarsudil and 3 H-AR-12286 were prepared by Quotient Bioresearch (Cambridgeshire, United Kingdom) by incorporating a 14 C label into the β position of the amino acid portion of netarsudil and a 3 H label at the C-5 of the isoquinolone, respectively.

    Techniques: Incubation, Staining, Fluorescence, Cell Adhesion Assay

    Netarsudil blocks the profibrotic effects of TGF-β on HTM cells. Serum-starved primary HTM cells incubated for 24 h in the presence of either vehicle, 8 ng/mL human TGF-β2, 500 nM netarsudil, or 8 ng/mL TGF-β2 plus 500 nM netarsudil were fixed and stained for the fibrogenic markers α-SMA, fibroblast-specific protein 1 (FSP1), and Collagen 1A. α-SMA, α-smooth muscle actin; HTM, human trabecular meshwork; TGF-β2, transforming growth factor-β2.

    Journal: Journal of Ocular Pharmacology and Therapeutics

    Article Title: Discovery and Preclinical Development of Netarsudil, a Novel Ocular Hypotensive Agent for the Treatment of Glaucoma

    doi: 10.1089/jop.2017.0023

    Figure Lengend Snippet: Netarsudil blocks the profibrotic effects of TGF-β on HTM cells. Serum-starved primary HTM cells incubated for 24 h in the presence of either vehicle, 8 ng/mL human TGF-β2, 500 nM netarsudil, or 8 ng/mL TGF-β2 plus 500 nM netarsudil were fixed and stained for the fibrogenic markers α-SMA, fibroblast-specific protein 1 (FSP1), and Collagen 1A. α-SMA, α-smooth muscle actin; HTM, human trabecular meshwork; TGF-β2, transforming growth factor-β2.

    Article Snippet: 14 C-netarsudil and 3 H-AR-12286 were prepared by Quotient Bioresearch (Cambridgeshire, United Kingdom) by incorporating a 14 C label into the β position of the amino acid portion of netarsudil and a 3 H label at the C-5 of the isoquinolone, respectively.

    Techniques: Incubation, Staining

    Netarsudil dose-dependent lowering of IOP in rabbits and monkeys. Formulations containing 0.005% (rabbit only), 0.01%, 0.02%, or 0.04% netarsudil were administered once daily (AM) to 1 eye of each animal for 3 days, with the fellow untreated eye serving as the control. IOP was determined for both eyes before test article administration (time 0) and at times 1, 2, 4, 8, and 24 h (rabbits) or 4, 8, and 24 h (monkeys) after each morning dose on Day 1 and 3. (A) Changes in IOP for the treated eye relative to the untreated contralateral eye in Dutch Belted rabbits ( n = 10/group). IOP reductions were statistically significant ( P

    Journal: Journal of Ocular Pharmacology and Therapeutics

    Article Title: Discovery and Preclinical Development of Netarsudil, a Novel Ocular Hypotensive Agent for the Treatment of Glaucoma

    doi: 10.1089/jop.2017.0023

    Figure Lengend Snippet: Netarsudil dose-dependent lowering of IOP in rabbits and monkeys. Formulations containing 0.005% (rabbit only), 0.01%, 0.02%, or 0.04% netarsudil were administered once daily (AM) to 1 eye of each animal for 3 days, with the fellow untreated eye serving as the control. IOP was determined for both eyes before test article administration (time 0) and at times 1, 2, 4, 8, and 24 h (rabbits) or 4, 8, and 24 h (monkeys) after each morning dose on Day 1 and 3. (A) Changes in IOP for the treated eye relative to the untreated contralateral eye in Dutch Belted rabbits ( n = 10/group). IOP reductions were statistically significant ( P

    Article Snippet: 14 C-netarsudil and 3 H-AR-12286 were prepared by Quotient Bioresearch (Cambridgeshire, United Kingdom) by incorporating a 14 C label into the β position of the amino acid portion of netarsudil and a 3 H label at the C-5 of the isoquinolone, respectively.

    Techniques: